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Effect of Salmonella enterica ser. Typhi on the gut population of Lactobacillus spp. among typhoid patients in Ondo State, Nigeria 肠道沙门氏菌的作用。伤寒对尼日利亚翁多州伤寒患者肠道乳酸杆菌种群的影响
IF 0.3 Q4 MICROBIOLOGY Pub Date : 2023-08-01 DOI: 10.21161/mjm.220165
Enabulele Onaiwu, Fadare Olalekan Shadrach
Aims: Aberrant gut microbiota has been linked to frequent exposure to enteric pathogens, a condition capable of causing various adverse effects on human health. In this study, we looked into how the typhoid fever condition might affect the lactobacillus population in the human gut. Methodology and results: A total of 424 faecal samples were collected from consented participants, who included 191 patients and 233 apparently healthy individuals. Lactobacilli and Salmonella enterica ser. Typhi ( S . Typhi) was isolated from samples cultured on de Man Rogosa and Sharpe agar (MRS) and xylose lysine deoxycholate agar (XLD), respectively. The overall prevalence of S. Typhi was 104(24.5%), of which 83(43.5%) were patients and 21(9.0%) were healthy controls. Lactobacillus spp. load in patients was significantly lower among the febrile, S . Typhi-positive patients with an average population of 5.5 ± 0.96 log 10 cfu/g compared with the febrile, but S . Typhi-negative patients with 6.41 ± log 10 cfu/g and the apparently healthy respondents with 7.34 ± 1.1 log 10 cfu/g. All the randomly selected S . Typhi strains obtained from both groups of respondents were sensitive to tetracycline and trimethoprim but resistant to chloramphenicol. Resistance to ciprofloxacin (18.2%) and ofloxacin (9.1%) was observed among the strains isolated from the febrile typhoid patients. Conclusion, significance and impact of study: This study has demonstrated an association between the population of Lactobacillus spp. and the presence of S . Typhi in the human gut. In order to ensure the recovery of beneficial bacteria during and after the treatment of infections, it is crucial to promote critical research into new treatment methods.
目的:异常的肠道微生物群与频繁接触肠道病原体有关,这是一种能够对人体健康造成各种不利影响的情况。在这项研究中,我们研究了伤寒状况如何影响人类肠道中的乳酸杆菌种群。方法和结果:从同意的参与者中共收集了424份粪便样本,其中包括191名患者和233名明显健康的个体。乳酸菌和肠道沙门氏菌。伤寒(S。分别从de Man Rogosa和Sharpe琼脂(MRS)和木糖赖氨酸脱氧胆酸琼脂(XLD)培养的样品中分离到伤寒杆菌。伤寒沙门氏菌总患病率104例(24.5%),其中患者83例(43.5%),健康对照21例(9.0%)。发热组患者乳酸杆菌载量明显降低。斑疹伤寒阳性人群与发热人群相比平均为5.5±0.96 log 10 cfu/g;typi阴性患者为6.41±log 10 cfu/g,而表面健康的应答者为7.34±1.1 log 10 cfu/g。所有随机选择的S。从两组应答者中获得的伤寒菌株对四环素和甲氧苄啶敏感,但对氯霉素耐药。对环丙沙星(18.2%)和氧氟沙星(9.1%)的耐药率最高。结论、意义和影响:本研究证实了乳酸菌种群与S。人类肠道中的伤寒。为了确保在感染治疗期间和之后有益菌的恢复,促进新的治疗方法的关键研究至关重要。
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引用次数: 0
The bactericidal effect of green macroalgae Cladophora sp. from freshwater toward Gram-negative and Gram-positive bacteria 淡水大绿藻Cladophora sp.对革兰氏阴性和革兰氏阳性细菌的杀菌作用
IF 0.3 Q4 MICROBIOLOGY Pub Date : 2023-08-01 DOI: 10.21161/mjm.220075
Amel Saoudi, M. Bouacha, S. Amri, H. Benbouzid, Khedidja Boufligha, S. Belhaoues, Karima Boutarfa, Meriem Abdelkrim, Iness Abdouni, Mourad Bensouilah
Aims: The development of new antimicrobial agents towards multidrug-resistant bacteria is one of the most significant challenges facing the healthcare system today. The continuous increase of antimicrobial resistance rates worldwide is a significant threat to public health. Therefore, this study aimed to investigate the antibacterial effect of filamentous macroalgae Cladophora sp. The sample was collected from an Algerian fountainhead of fresh water. A crude hydromethanolic extract (methanol-water) was tested against two standard Gram-negative bacteria: Escherichia coli ATCC25922 and Pseudomonas aeruginosa ATCC27853 , and two standard Gram-positive bacteria: Staphylococcus aureus ATCC25923 and Enterococcus faecalis ATCC 2921. Methodology and results: The antibacterial effect of the hydromethanolic extract of Cladophora sp. was investigated using the well diffusion method to determine the inhibitory diameters and the dilution methods to determine the minimum inhibitory concentration (MIC) and the bactericidal inhibitory concentration (MBC). The results indicated that the hydromethanolic extract of Cladophora sp. is more effective towards Gram-positive bacteria, with a significant effect on S. aureus ATCC 25923, where an inhibitory diameter of 35 mm was recorded. For the Gram-negative, E. coli ATCC25922 was more susceptible with inhibitory diameters of 46 mm, followed by P. aeruginosa ATCC 27853 with 28 mm. The MIC value of hydromethanolic extract of Cladophora sp. was 50 µg/mL for Gram-positive bacteria ( S. aureus ATCC25923 and E. faecalis ATCC29212). However, it was 100 µg/mL for Gram-negative bacteria ( E. coli ATCC25922 and P. aeruginosa ATCC 27853). The best bactericidal effect was observed with Gram-positive with an MBC of 100 µg/mL. The MBC for Gram-negative bacteria was 150 µg/mL. Conclusion, significance and impact of study: The Cladophora sp. macroalgae represent a potential source of bioactive compounds, which could be used in the management and treatment of various microbial infections.
目的:针对多药耐药细菌开发新的抗菌药物是当今卫生保健系统面临的最重大挑战之一。世界范围内抗菌素耐药率的持续上升是对公共卫生的重大威胁。因此,本研究旨在探讨丝状大藻Cladophora sp.的抗菌作用。样品采集自阿尔及利亚的一处淡水水源。采用甲醇-水粗提物对2种标准革兰氏阴性菌(大肠杆菌ATCC25922、铜绿假单胞菌ATCC27853)和2种标准革兰氏阳性菌(金黄色葡萄球菌ATCC25923、粪肠球菌ATCC 2921)进行抑菌试验。方法与结果:采用孔扩散法测定其抑菌直径,稀释法测定其最小抑菌浓度(MIC)和杀菌抑菌浓度(MBC),考察其抑菌效果。结果表明,Cladophora sp. hydrome甲醇提取物对革兰氏阳性菌的抑菌效果更好,对金黄色葡萄球菌ATCC 25923的抑菌效果显著,抑菌直径为35 mm。对于革兰氏阴性菌,大肠杆菌ATCC25922最敏感,抑制直径为46 mm,铜绿假单胞菌ATCC 27853次之,抑制直径为28 mm。革兰氏阳性菌金黄色葡萄球菌(S. aureus) ATCC25923和粪肠球菌(E. faecalis) ATCC29212的MIC值为50µg/mL。而革兰氏阴性菌(大肠杆菌ATCC25922和铜绿假单胞菌atcc27853)为100µg/mL。革兰氏阳性菌的杀菌效果最好,MBC为100µg/mL。革兰氏阴性菌的MBC为150µg/mL。结论、研究意义及影响:Cladophora sp. macroalgae是一种潜在的生物活性化合物来源,可用于各种微生物感染的管理和治疗。
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引用次数: 0
Colonization and antagonistic activity of endophytic fungi in seagrasses: Understanding endophyte interaction 海草内生真菌的定植和拮抗活性:了解内生真菌相互作用
IF 0.3 Q4 MICROBIOLOGY Pub Date : 2023-08-01 DOI: 10.21161/mjm.220097
V. Kinamot, Bantoto, Alvin Monotilla, Puspus
Endophytic fungal colonization in plants is governed by complex interactions with the defense mechanism of the host and antagonistic effects of other endophytes. In this study, endophytic fungal interaction was assessed by histological examination and co-culture methods. Results showed fungal colonization in the intercellular space of the epidermis and both intercellular and intracellular spaces of the cortical cells suggesting close interaction with their seagrass hosts. Dense colonization, hyphal branching, coiling and formation of networks were observed in the cortical cells. Less competition for space and reliable source of nutrition in the cortex may favor fungal growth. No fungal hyphae were detected in the vascular tissues of seagrasses. All the endophytic fungi isolated from seagrasses showed antagonistic activity. Aspergillus tamarii, A. ochraceopetaliformis, Penicillium citrinum, Beauveria bassiana, Eutypella sp. and Xylaria sp were the most active antagonists. Antagonistic interaction involved deadlock and replacement. Deadlock was associated with physical blocking of the antagonist’s colony by hyphal aggregation and production of inhibitory metabolites. Demarcation line and colony pigmentation in Xylaria sp. during co-culture assay indicated the production of high quantities of inhibitory molecules. Endophytic fungi in seagrasses also produced volatile organic compounds (VOC) which resulted to deadlock at mycelial distance. Thus, endophyte colonization and distribution in seagrass tissues are in�uenced by their interaction with the hosts and other endophytes. But interestingly, cyclical intransitivity of multispecies interaction manifested by these fungal species suggested possible co-existence in seagrass tissues.
植物内生真菌的定植受寄主防御机制和其他内生菌拮抗作用的复杂相互作用支配。本研究通过组织学检查和共培养方法评估内生真菌相互作用。结果表明,真菌在表皮的细胞间隙和皮层细胞的细胞间隙和细胞内都有定植,表明它们与海草宿主的相互作用密切。在皮层细胞中观察到密集的定植、菌丝分支、盘绕和网络的形成。较少的空间竞争和皮层中可靠的营养来源可能有利于真菌的生长。海草维管组织中未检出真菌菌丝。从海草中分离得到的内生真菌均表现出拮抗活性。柽柳曲霉、青霉、柑橘青霉、球孢白僵菌、真苔菌和木霉的拮抗作用最强。拮抗相互作用包括死锁和替代。死锁与拮抗菌落通过菌丝聚集和产生抑制性代谢物的物理阻断有关。在共培养实验中,木蝇的分界线和菌落色素沉着表明产生了大量的抑制分子。海草内生真菌也产生挥发性有机化合物(VOC),导致菌丝距离死锁。因此,内生菌在海草组织中的定植和分布受其与宿主和其他内生菌的相互作用的影响。但有趣的是,这些真菌物种所表现出的多物种相互作用的周期性非传递性表明可能在海草组织中共存。
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引用次数: 0
Characterization of bacterial communities in prebiotics and probiotics treated shrimp farms from Kuantan 关丹省益生元和益生菌处理对虾养殖场细菌群落特征
IF 0.3 Q4 MICROBIOLOGY Pub Date : 2023-08-01 DOI: 10.21161/mjm.220048
Shing Wei Siew, Ira Nadira Kosnin, Nurul Fadzlina Zulkifli, Hajar Fauzan, Normaiza Zamri, Muhammad Fitri Yusof, Ahmad M. Gazali
Aims: Prebiotics and probiotics profoundly enhance water quality and shrimp development to tackle infectious disease in shrimp farming. This study evaluated the impact of prebiotics and probiotics treatments in water by assessing the physicochemical properties and bacterial communities in local shrimp ponds. Methodology and results: Water was collected from shrimp pond 1 (SP1), treated with prebiotics and probiotics, and shrimp pond 2 (SP2), treated with only prebiotics. The physicochemical parameters of water from two shrimp ponds were measured, including pH, dissolved oxygen (DO), ammonia concentration and temperature. The total environmental DNA (eDNA) was extracted from the water samples and sequenced using amplicon sequencing targeting the full length of the 16S rRNA gene region via the Oxford Nanopore Technology Flongle. The water quality analysis indicated that SP1 had better water quality than SP2 for shrimp aquaculture. The dominant phyla in both shrimp ponds were Proteobacteria and Bacteroidota . SP1 samples had unique microbiota at the phylum level, including Bdellovibrionota , Firmicutes A , Patescibacteria and unclassified Rhizobiales , Saprospiraceae , Vulcanococcus and HIMB114 at the genus level. The alpha-and beta-diversity showed insignificant differences in microbiota composition between SP1 and SP2 (p-value>0.05). Conclusion, significance and impact of study: Research findings demonstrated that the probiotic-treated shrimp pond (SP1) had better water quality and more diverse microbial communities than the shrimp pond that was not treated with probiotics (SP2).
目的:益生元和益生菌能深刻改善水质和对虾发育,解决对虾养殖中的传染病问题。本研究通过对当地虾池的理化性质和细菌群落进行评估,评价了益生元和益生菌处理对水体的影响。方法与结果:从1号虾池(SP1)和2号虾池(SP2)收集水,分别用益生元和益生菌处理和单独用益生元处理。测定了两个对虾池水的理化参数,包括pH、溶解氧(DO)、氨浓度和温度。从水样中提取总环境DNA (eDNA),并通过牛津纳米孔技术Flongle对16S rRNA基因区域的全长进行扩增子测序。水质分析表明,SP1养殖对虾的水质优于SP2。两个虾池的优势门均为变形菌门和拟杆菌门。SP1样品在门水平上具有独特的微生物群,包括Bdellovibrionota、厚壁菌门A、Patescibacteria和属水平上未分类的Rhizobiales、Saprospiraceae、Vulcanococcus和HIMB114。SP1和SP2的α和β微生物群组成差异不显著(p值>0.05)。结论、研究意义及影响:研究结果表明,与未添加益生菌(SP2)的对虾池相比,添加了益生菌的对虾池(SP1)水质更好,微生物群落更多样化。
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引用次数: 0
Antimicrobial activity of novel Cobalt(II) complexes with Schiff base derived from L-cysteine and 2-substituted benzaldehyde 由l -半胱氨酸和2-取代苯甲醛衍生的新型希夫碱钴(II)配合物的抗菌活性
IF 0.3 Q4 MICROBIOLOGY Pub Date : 2023-08-01 DOI: 10.21161/mjm.220135
M. Pazalja, I. Mahmutović-Dizdarević, Sabina Begić, Alisa Smajović, A. Jerković-Mujkić, M. Avdic, M. Salihović
Aims: The aim of this study was to conduct antimicrobial analysis on novel Schiff base-derived cobalt(II) complexes (Co(L1) 2 and Co(L2) 2 )
目的:本研究旨在对新型希夫碱衍生钴(II)配合物(Co(L1) 2和Co(L2) 2)进行抗菌分析。
{"title":"Antimicrobial activity of novel Cobalt(II) complexes with Schiff base derived from L-cysteine and 2-substituted benzaldehyde","authors":"M. Pazalja, I. Mahmutović-Dizdarević, Sabina Begić, Alisa Smajović, A. Jerković-Mujkić, M. Avdic, M. Salihović","doi":"10.21161/mjm.220135","DOIUrl":"https://doi.org/10.21161/mjm.220135","url":null,"abstract":"Aims: The aim of this study was to conduct antimicrobial analysis on novel Schiff base-derived cobalt(II) complexes (Co(L1) 2 and Co(L2) 2 )","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"10 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74314613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oncolytic effect of Newcastle disease virus (NDV) strain AF2240 and V4-UPM on hypoxic and normoxic osteosarcoma cells 新城疫病毒(NDV) AF2240株和V4-UPM对缺氧和常氧骨肉瘤细胞的溶瘤作用
IF 0.3 Q4 MICROBIOLOGY Pub Date : 2023-08-01 DOI: 10.21161/mjm.230191
Nurneqman Nashreq Kosni, Noraini Abd-Aziz, Abdul Rahman Omar, Saila Ismail, Suet Lin
Aims: Hypoxia is believed to be one of the key components contributing to the clinical resistance of cancer therapies. Alternative strategies are under investigation to overcome this resistance and the oncolytic virus stands amongst the others. Newcastle disease virus (NDV) has been demonstrated to possess oncolytic activity against cancer cells. The present study investigated the effects of oncolytic NDV strain AF2240 and V4-UPM on osteosarcoma cells (Saos-2) under normoxic and hypoxic conditions
目的:缺氧被认为是癌症治疗临床耐药的关键因素之一。目前正在研究克服这种耐药性的替代策略,溶瘤病毒是其中之一。新城疫病毒(NDV)已被证实对癌细胞具有溶瘤活性。本研究研究了溶瘤性NDV菌株AF2240和V4-UPM在常氧和缺氧条件下对骨肉瘤细胞(Saos-2)的影响
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引用次数: 0
Detection of rpoB gene mutations in clinical isolates of Rifampicin-resistant Mycobacterium tuberculosis in Makassar City, South Sulawesi, Indonesia 印度尼西亚南苏拉威西望加锡市耐利福平结核分枝杆菌临床分离株rpoB基因突变检测
IF 0.3 Q4 MICROBIOLOGY Pub Date : 2023-08-01 DOI: 10.21161/mjm.220133
Muttaqin, Z., Massi, M. N., Sjahril, R., Halik, H., Junaedi, M. A., Rifqiani, N., Safitri, N. I., Pratika, M., Islam, I. C., Hamid, F., Natzir, R., Ahmad, A.
{"title":"Detection of rpoB gene mutations in clinical isolates of Rifampicin-resistant Mycobacterium tuberculosis in Makassar City, South Sulawesi, Indonesia","authors":"Muttaqin, Z., Massi, M. N., Sjahril, R., Halik, H., Junaedi, M. A., Rifqiani, N., Safitri, N. I., Pratika, M., Islam, I. C., Hamid, F., Natzir, R., Ahmad, A.","doi":"10.21161/mjm.220133","DOIUrl":"https://doi.org/10.21161/mjm.220133","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"88 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82380609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Viral disease control in strawberry plants: A potential impact of green nanotechnology 草莓植物的病毒病控制:绿色纳米技术的潜在影响
IF 0.3 Q4 MICROBIOLOGY Pub Date : 2023-06-01 DOI: 10.21161/mjm.220063
Shafie, R. M., Abdelkader, H. S.
{"title":"Viral disease control in strawberry plants: A potential impact of green nanotechnology","authors":"Shafie, R. M., Abdelkader, H. S.","doi":"10.21161/mjm.220063","DOIUrl":"https://doi.org/10.21161/mjm.220063","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"4 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74572105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A review of current trends of antibacterial Schiff base complexes: Lower and higher transition metal complexes 抗菌席夫碱配合物的研究进展:低过渡金属配合物和高过渡金属配合物
IF 0.3 Q4 MICROBIOLOGY Pub Date : 2023-06-01 DOI: 10.21161/mjm.220103
Aziza Sarwar, H. Bahron, Bibi Sherino, Anila Ali, Sajjad Bhangwar, Y. Alias
The development of metal complexes has inspired researchers to progress in this domain due to their extensive applications in the biological field. Regarding the application, binuclear metal complexes are less explored than their mononuclear counterparts. Recent development in transition metal Schiff base complexes was outlined and presented in detail with their respective vast applications, especially antibacterial. The relationship of their structure, functions, properties
金属配合物在生物领域的广泛应用激发了研究人员在这一领域的进步。在应用方面,双核金属配合物比单核金属配合物探索较少。介绍了近年来过渡金属席夫碱配合物的研究进展及其在抗菌方面的广泛应用。它们的结构,功能,性质之间的关系
{"title":"A review of current trends of antibacterial Schiff base complexes: Lower and higher transition metal complexes","authors":"Aziza Sarwar, H. Bahron, Bibi Sherino, Anila Ali, Sajjad Bhangwar, Y. Alias","doi":"10.21161/mjm.220103","DOIUrl":"https://doi.org/10.21161/mjm.220103","url":null,"abstract":"The development of metal complexes has inspired researchers to progress in this domain due to their extensive applications in the biological field. Regarding the application, binuclear metal complexes are less explored than their mononuclear counterparts. Recent development in transition metal Schiff base complexes was outlined and presented in detail with their respective vast applications, especially antibacterial. The relationship of their structure, functions, properties","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"32 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89077569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of culture conditions on pyocyanin production by recombinant pyocyanin-producing strain Pseudomonas aeruginosa PS39-phzMS 培养条件对重组铜绿假单胞菌PS39-phzMS产花青素的影响
IF 0.3 Q4 MICROBIOLOGY Pub Date : 2023-06-01 DOI: 10.21161/mjm.220077
Nguyen, V. Q., Nguyen, U. H., Nguyen, T. C., Dao, A. T. N., Nguyen, L. T. T.
{"title":"Effect of culture conditions on pyocyanin production by recombinant pyocyanin-producing strain Pseudomonas aeruginosa PS39-phzMS","authors":"Nguyen, V. Q., Nguyen, U. H., Nguyen, T. C., Dao, A. T. N., Nguyen, L. T. T.","doi":"10.21161/mjm.220077","DOIUrl":"https://doi.org/10.21161/mjm.220077","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"12 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79260072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Malaysian Journal of Microbiology
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