Mahalingam, S. R. R., Peremalo, T., Madhavan, P., Hamzah, S., Than, L. T. L., Chong, P. P., Cheah, Y. K., Santhanam, J., James, J. E.
{"title":"Analysis of Internal Transcribed Spacer (ITS) region and D1/D2 domain coupled with Random Amplified Polymorphic DNA (RAPD) reveal the inter- and intraspecific relationships of Diutina rugosa and Diutina mesorugosa isolated from Malaysian patients","authors":"Mahalingam, S. R. R., Peremalo, T., Madhavan, P., Hamzah, S., Than, L. T. L., Chong, P. P., Cheah, Y. K., Santhanam, J., James, J. E.","doi":"10.21161/mjm.221449","DOIUrl":"https://doi.org/10.21161/mjm.221449","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"70 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73823455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation, identification and anti-fungal susceptibility pattern of dermatophytes from clinical samples","authors":"Sehgal, P., Singh, K.","doi":"10.21161/mjm.220159","DOIUrl":"https://doi.org/10.21161/mjm.220159","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"42 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90652803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nur A’fina, Ahmad Mokhtar, Darah Ibrahim, Siti Shahara Zulfakar, L. Zakaria, Kamarul Zaman
Aims: The antibiofilm activity of endophytic fungus Nigrospora sphaerica CL-OP30 isolated from Swietenia macrophylla King was investigated. Methodology and results: The ability of the fungal endophytic crude extract to impede Streptococcus mutans biofilm formation was preliminarily screened with Congo red agar test. It was proven that S. mutans biofilm formation was hindered on the agar supplemented with the fungal endophytic crude extract. The antibiofilm activity of the fungal endophytic crude extract was evaluated using a microtiter plate method on both initially formed and preformed biofilm. Antibiofilm activity was recorded in a concentration-dependent pattern whereby higher concentrations reduced biofilm formation better than the lower concentrations of extract for both initially formed and preformed biofilm. The architecture of biofilm tested with fungal endophytic crude extract was also observed. Visualization under a light microscope and SEM revealed that the adherence of S. mutans biofilm treated with fungal endophytic crude extract was significantly reduced in both initially formed and preformed biofilm. In addition, observation under SEM showed that the matrices surrounding the bacterial cells were disintegrated and bacterial cells in biofilm completely lost their original shape. The overall data demonstrated that the ethyl acetate N. spaherica CL-OP30 crude extract showed good antibiofilm activity. Conclusion, significance and impact of study: The antibiofilm study suggested the potential of N. sphaerica CL-OP30 crude extract against S. mutans biofilm by disrupting the biofilm formation, the disintegration of matrices surrounding the biofilm and responsible for the formation of irregular cell shape. This extract may have a promising potential to be developed as an antibiofilm agent.
{"title":"Antibiofilm activity of Nigrospora sphaerica CL-OP30 endophytic extract against Streptococcus mutans - the causative agent of dental caries","authors":"Nur A’fina, Ahmad Mokhtar, Darah Ibrahim, Siti Shahara Zulfakar, L. Zakaria, Kamarul Zaman","doi":"10.21161/mjm.230203","DOIUrl":"https://doi.org/10.21161/mjm.230203","url":null,"abstract":"Aims: The antibiofilm activity of endophytic fungus Nigrospora sphaerica CL-OP30 isolated from Swietenia macrophylla King was investigated. Methodology and results: The ability of the fungal endophytic crude extract to impede Streptococcus mutans biofilm formation was preliminarily screened with Congo red agar test. It was proven that S. mutans biofilm formation was hindered on the agar supplemented with the fungal endophytic crude extract. The antibiofilm activity of the fungal endophytic crude extract was evaluated using a microtiter plate method on both initially formed and preformed biofilm. Antibiofilm activity was recorded in a concentration-dependent pattern whereby higher concentrations reduced biofilm formation better than the lower concentrations of extract for both initially formed and preformed biofilm. The architecture of biofilm tested with fungal endophytic crude extract was also observed. Visualization under a light microscope and SEM revealed that the adherence of S. mutans biofilm treated with fungal endophytic crude extract was significantly reduced in both initially formed and preformed biofilm. In addition, observation under SEM showed that the matrices surrounding the bacterial cells were disintegrated and bacterial cells in biofilm completely lost their original shape. The overall data demonstrated that the ethyl acetate N. spaherica CL-OP30 crude extract showed good antibiofilm activity. Conclusion, significance and impact of study: The antibiofilm study suggested the potential of N. sphaerica CL-OP30 crude extract against S. mutans biofilm by disrupting the biofilm formation, the disintegration of matrices surrounding the biofilm and responsible for the formation of irregular cell shape. This extract may have a promising potential to be developed as an antibiofilm agent.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"116 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88850539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Akpanke, J., Henry, I. I., Ugbadu, J. I., Abuh, S. A.
{"title":"In vitro antibiotics resistance patterns of selected genera of bacteria from waste collection sites in University of Calabar campus, Cross River State, Nigeria","authors":"Akpanke, J., Henry, I. I., Ugbadu, J. I., Abuh, S. A.","doi":"10.21161/mjm.220049","DOIUrl":"https://doi.org/10.21161/mjm.220049","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"70 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77910695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Musa, A. M. I., Fagir, N. E. A., Al-khadher, M. A., Alshahrani, M. A., Nahari, M. H., Motawa, M. E. S., Ahmed, I. A. M.
{"title":"Prevalence of methicillin-resistant Staphylococcus aureus on cellphones of healthcare workers in Najran University hospital, Najran City (Saudi Arabia)","authors":"Musa, A. M. I., Fagir, N. E. A., Al-khadher, M. A., Alshahrani, M. A., Nahari, M. H., Motawa, M. E. S., Ahmed, I. A. M.","doi":"10.21161/mjm.220047","DOIUrl":"https://doi.org/10.21161/mjm.220047","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"41 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84678220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
None Stanley, S., None Lihan, S., None Benet, F. B., None Ramih, S., None Hamdi, K. B. M.
{"title":"Isolation and molecular characterization of bacterial species from Sikog waterfall, Padawan, Sarawak","authors":"None Stanley, S., None Lihan, S., None Benet, F. B., None Ramih, S., None Hamdi, K. B. M.","doi":"10.21161/mjm.221552","DOIUrl":"https://doi.org/10.21161/mjm.221552","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135094947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bioconversion of Cotton Fibers Wastes to Ethanol by Fungal Cellulases","authors":"Karam, E. A. H., Elattal, N. A., Kansoh, A. L.","doi":"10.21161/mjm.220072","DOIUrl":"https://doi.org/10.21161/mjm.220072","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"88 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77472620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Okoro, C. U., Obeten, S., John, G. E., Lennox, J. A.
{"title":"Inhibitory effect of lactic acid bacteria on Helicobacter pylori in vitro","authors":"Okoro, C. U., Obeten, S., John, G. E., Lennox, J. A.","doi":"10.21161/mjm.220030","DOIUrl":"https://doi.org/10.21161/mjm.220030","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"35 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81588792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohd-Aspar, M. A. S., Edros, R., Mokhtar, S. U., Kamarudin, N.
{"title":"The roles of acidity, peroxide and non-peroxide compounds in antibacterial properties of Malaysian Kelulut, Tualang and Acacia honey","authors":"Mohd-Aspar, M. A. S., Edros, R., Mokhtar, S. U., Kamarudin, N.","doi":"10.21161/mjm.220084","DOIUrl":"https://doi.org/10.21161/mjm.220084","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"37 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83842776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Barata, D. M., Ruslan, N. A., Zawawi, N. A. A. M., Kub, T. N. T., Alojid, A. A. M.
{"title":"Comparative antibacterial activity and stability of Andrographis paniculata herbal mouthwash and commercial mouthwashes","authors":"Barata, D. M., Ruslan, N. A., Zawawi, N. A. A. M., Kub, T. N. T., Alojid, A. A. M.","doi":"10.21161/mjm.220052","DOIUrl":"https://doi.org/10.21161/mjm.220052","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"254 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76293151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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