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Gelatin hydrolysate in freeze–thawed shrimp model system: retardation of weight loss and muscle protein denaturation 冻融虾模型系统中的明胶水解物:延缓体重下降和肌肉蛋白质变性
IF 3.3 3区 农林科学 Q1 Engineering Pub Date : 2024-05-22 DOI: 10.1111/ijfs.17227
Phanat Kittiphattanabawon, Wattana Temdee, Sajid Maqsood, Wonnop Visessanguan, Soottawat Benjakul

This research explores the cryoprotective potential of gelatin hydrolysate by determining weight loss and physicochemical changes in the muscle protein of freeze–thawed shrimp. Shrimp treated with gelatin hydrolysate exhibited significantly higher and lower weight gain (9.20%) than those treated with distilled water (0.93%) and mixed phosphates (16.99%), respectively. Gelatin hydrolysate could lower the increasing rate of thawing and cooking loss and freezable water content in freeze–thawed shrimp (P < 0.05) and comparable to those of mixed phosphates treated shrimp (P > 0.05). As the shrimp were subjected to freeze–thawing, gelatin hydrolysate slowed the decline of protein solubility and Ca2+-ATPase activity and mitigated the rise of surface hydrophobicity and disulfide bond content. It also delayed the shear force increase. These findings suggest that gelatin hydrolysate could be an effective alternative to traditional cryoprotectants, retarding weight loss and protein denaturation to preserve shrimp quality during freezing and frozen storage.

本研究通过测定冻融对虾的体重减轻和肌肉蛋白质的理化变化,探索明胶水解物的低温保护潜力。与蒸馏水(0.93%)和混合磷酸盐(16.99%)相比,用明胶水解物处理的对虾的增重(9.20%)明显较高,增重(9.20%)较低。明胶水解物可以降低冻融虾解冻和蒸煮损失的增加率以及可冻结的含水量(P 0.05)。在对虾冻融过程中,明胶水解物减缓了蛋白质溶解度和 Ca2+-ATP 酶活性的下降,缓解了表面疏水性和二硫键含量的上升。它还能延缓剪切力的增加。这些研究结果表明,明胶水解物可以有效替代传统的冷冻保护剂,在冷冻和冷藏过程中延缓重量损失和蛋白质变性,从而保持虾的品质。
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引用次数: 0
Influence of flour extraction rate on static in vitro gastrointestinal digestion characteristics of wheat protein during conventional milling 面粉提取率对传统制粉过程中小麦蛋白质体外胃肠道静态消化特性的影响
IF 3.3 3区 农林科学 Q1 Engineering Pub Date : 2024-05-20 DOI: 10.1111/ijfs.17184
Lingfang Zhang, Shuzhen Yang, Binghua Sun, Xiaoxi Wang, Sen Ma

The flour extraction rate determines the components of wheat flour. However, the correlation between variations in components resulting from flour extraction rate and the protein digestion characteristics remains to be clarified. In this study, digestion characteristic of wheat flour protein influenced by flour extraction rates was investigated. The results indicated that the degree of protein hydrolysis, the number of peptides at the intestinal stage and the digestive rate constant (k) at the gastric stage exhibited a significant increase as the flour extraction rate increased (35%–85%). Moreover, wheat flour with 85% extraction rate presented the highest protein hydrolysis degree (25.85%), the most peptides (n = 79) and the maximum intestinal digestion rate constant (k = 0.0285). Therefore, increasing the flour extraction rate from 75% to 85% can improve the wheat flour protein digestibility in conventional milling, thereby establishing a fundamental basis for nutritional control of flour products.

面粉提取率决定了小麦粉的成分。然而,面粉提取率导致的成分变化与蛋白质消化特性之间的相关性仍有待明确。本研究调查了面粉提取率对小麦粉蛋白质消化特性的影响。结果表明,随着面粉萃取率的增加(35%-85%),蛋白质的水解程度、肠道阶段的肽数和胃肠阶段的消化率常数(k)均有显著增加。此外,提取率为 85% 的小麦粉蛋白质水解度最高(25.85%),肽数最多(n = 79),肠道消化率常数最大(k = 0.0285)。因此,将面粉提取率从 75% 提高到 85%,可以提高小麦粉蛋白质在传统制粉工艺中的消化率,从而为面粉产品的营养控制奠定基础。
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引用次数: 0
Quality enhancement of sweet potato puree oat mixed-grain noodles based on curdlan: recommended addition level and mechanism 基于可得然的甘薯泥燕麦杂粮面条的质量提升:建议添加量和机理
IF 3.3 3区 农林科学 Q1 Engineering Pub Date : 2024-05-17 DOI: 10.1111/ijfs.17218
Xinyu Wei, Guangyue Ren, Xu Duan, Linlin Li, Weiwei Cao, Junliang Chen, Xiaofei Sun, Linlin Zhao, Wenchao Liu

The inherent poor cooking quality and poor texture of mixed-grain pasta limit consumer acceptance. This study systematically investigated the effect of curdlan in the processing of sweet potato puree oat mixed-grain noodles, and comprehensively analysed a number of key indicators such as cooking characteristics, moisture state, texture properties, microstructure, starch crystallinity characteristics, short-range ordered structure and thermal properties. At the production practice level, the study clearly indicated that the 0.6% curdlan addition ratio was the best solution in sweet potato puree oat mixed-grain noodles processing. This finding not only significantly reduces the fracture rate, water absorption rate and cooking loss rate during the cooking process, but also significantly improves the quality and yield of the product, and brings significant improvement in the taste and texture of the noodles, which is of great significance for industrial production. In the microstructural and molecular studies, we revealed how curdlan could enhance the structural stability and mechanical strength of noodles by promoting the uniform distribution of starch granules in the gluten protein network. Further, through X-ray diffraction, ordered structure and thermal property analyses, we have found that colchicine can effectively regulate the interactions of hydrogen bonding between starch and the content of straight-chain and branched-chain starch, which significantly reduces the crystallinity, ordered short-chain structure and enthalpy of pasting of starch in the pasta. These in-depth research results not only help us to understand more comprehensively the formation mechanism of the modification of pasta quality by curdlan, but also provide a solid theoretical foundation and practical guidance for the improvement of pasta quality in mixed grains.

杂粮面条固有的烹饪质量差、口感差等问题限制了消费者的接受程度。本研究系统研究了可得然在红薯泥燕麦杂粮面加工中的应用效果,全面分析了红薯泥燕麦杂粮面的蒸煮特性、水分状态、质地特性、微观结构、淀粉结晶特性、短程有序结构和热性能等多项关键指标。在生产实践层面,研究清楚地表明,0.6% 的可得然添加比例是红薯泥燕麦杂粮面加工的最佳解决方案。这一发现不仅大大降低了蒸煮过程中的断裂率、吸水率和蒸煮损失率,还显著提高了产品的质量和产量,使面条的口感和质地得到明显改善,对工业化生产具有重要意义。在微观结构和分子研究中,我们揭示了可得然如何通过促进淀粉颗粒在面筋蛋白网络中的均匀分布来提高面条的结构稳定性和机械强度。此外,通过 X 射线衍射、有序结构和热性能分析,我们发现秋水仙碱能有效调节淀粉之间氢键的相互作用以及直链和支链淀粉的含量,从而显著降低面条中淀粉的结晶度、有序短链结构和糊化焓。这些深入的研究成果不仅有助于我们更全面地了解可得然对面食品质改性的形成机理,也为杂粮面食品质的改善提供了坚实的理论基础和实践指导。
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引用次数: 0
Elderberry pomace for food/nutraceutical product development: impact of drying methods and simulated in vitro gastrointestinal digestion on phenolic profile and bioaccessibility 用于食品/保健品开发的接骨木果渣:干燥方法和模拟体外胃肠道消化对酚类概况和生物可及性的影响
IF 3.3 3区 农林科学 Q1 Engineering Pub Date : 2024-05-17 DOI: 10.1111/ijfs.17221
Milagros Gomez Mattson, Rocío Corfield, Carolina Schebor, Daniela Salvatori

The aim of this work was to develop ingredients from elderberry pomace (EP) analysing the effect of different drying methods (conventional air-drying (C), freeze-drying (F), and fluidised-bed drying (FB)) on phenolic compounds retention and their bioaccessibility. The dry pomaces were chemically characterised before and after an in vitro gastrointestinal digestion (GID). Cianidin-3-glucoside and rutin were the main phenolic compounds determined by HPLC-DAD. After GID, phenolic content and antioxidant capacity were drastically reduced regardless of the drying process. Freeze-drying better preserved the total polyphenol and monomeric anthocyanin content, and the antioxidant capacity, however, EP-C and EP-FB presented more bioaccessible antioxidant compounds than EP-F. Fluidised-bed drying generated, in a shorter drying time, a more bioaccessible granular product (EP-FB), that could be used directly in ready-to-eat foods. EP-C and EP-F products could be used as functional foods or nutraceutical ingredients. Also, the obtained elderberry products were not cytotoxic against the Caco-2 cell line.

这项工作的目的是开发接骨木果渣(EP)配料,分析不同干燥方法(传统空气干燥法(C)、冷冻干燥法(F)和流化床干燥法(FB))对酚类化合物保留及其生物可及性的影响。在体外胃肠消化(GID)前后,对干果渣进行了化学鉴定。通过 HPLC-DAD 法测定的主要酚类化合物是翠菊素-3-葡萄糖苷和芦丁。胃肠消化后,无论干燥过程如何,酚含量和抗氧化能力都急剧下降。冷冻干燥能更好地保存总多酚和单体花青素含量以及抗氧化能力,但 EP-C 和 EP-FB 比 EP-F 能提供更多生物可利用的抗氧化化合物。流化床干燥法在较短的干燥时间内产生了生物可及性更高的颗粒状产品(EP-FB),可直接用于即食食品。EP-C 和 EP-F 产品可用作功能食品或营养保健成分。此外,获得的接骨木产品对 Caco-2 细胞系没有细胞毒性。
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引用次数: 0
Physicochemical and sensory characterisation of Salvator merianae meat as a sustainable alternative 作为可持续替代品的鼠尾草肉的理化和感官特征
IF 3.3 3区 农林科学 Q1 Engineering Pub Date : 2024-05-17 DOI: 10.1111/ijfs.17167
Micaela R. Mazaratti, Florencia E. Valli, Sofía E. Pierini, Marcela A. Gonzalez, Carlos I. Piña, Facundo Cuffia, Melina S. Simoncini, Pamela M.L. Leiva

Considering the escalating consumer preference for health-conscious commodities, reptile species' meat can be presented as an alternative animal protein source because of its notable nutritional characteristics. The aim of this study was to evaluate the physicochemical parameters (pH, tenderness, cooking loss, colour and fatty acid profile) and sensory profile of black-and-white Tegu meat. The black-and-white Tegu meat was assessed through to consumer-based sensory Analysis alongside the Yellow Anaconda, Broad-snouted Caiman and broilers through. The samples of reptile species' meat were obtained from sustainable use programs. In terms of physicochemical parameters, the results indicate that black-and-white Tegu meat exhibits moderate brightness, displaying a pale pink/yellowish tone. Oleic acid was the most abundant fatty acid in black-and-white Tegu meat, comprising approximately 22.5%, while the content of polyunsaturated fatty acids was approximately 34%. Regarding overall liking, all four types of meats scored higher than indifference. However, black-and-white Tegu meat presented a lower acceptability (P < 0.001), likely attributed to its dry texture and unpleasant colour, as perceived by consumers. Our findings underscore the need to explore preparation methods for enhancing the sensory characteristics of black-and-white Tegu meat. This will enable its promotion as a healthy food choice in the future. The utilisation of black-and-white Tegu meat as a food source will benefit families participating in the sustainable use and conservation program, the ‘Proyecto Tupinambis’.

考虑到消费者对注重健康的商品的偏好不断升级,爬行动物肉类因其显著的营养特性,可作为替代动物蛋白质来源。本研究的目的是评估黑白泰固犬肉的理化参数(pH 值、嫩度、烹饪损失、色泽和脂肪酸概况)和感官特征。黑白泰固犬肉与黄蟒、宽吻凯门鳄和肉鸡肉一起通过消费者感官分析进行评估。爬行动物肉类样品来自可持续利用项目。在理化参数方面,结果表明黑白泰谷肉的亮度适中,呈淡粉色/淡黄色。油酸是黑白泰固犬肉中含量最高的脂肪酸,约占 22.5%,而多不饱和脂肪酸的含量约为 34%。在总体喜好方面,所有四种肉类的得分都高于 "无所谓"。然而,黑白髭蟾肉的可接受性较低(P < 0.001),这可能是由于消费者认为其质地干燥、颜色难看。我们的研究结果表明,有必要探索提高黑白泰古肉感官特性的制备方法。这将有助于今后将其作为一种健康食品进行推广。将黑白泰格犬肉作为一种食物来源将使参与可持续利用和保护计划 "图皮南比斯计划 "的家庭受益。
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引用次数: 0
Assessing nutritional probing and storage stability of functional Aloe vera (Aloe barbadensis) based guava jam: a machine learning approach for predictive modelling 评估基于功能性芦荟(Aloe barbadensis)的番石榴果酱的营养探测和储存稳定性:预测建模的机器学习方法
IF 3.3 3区 农林科学 Q1 Engineering Pub Date : 2024-05-17 DOI: 10.1111/ijfs.17209
Hafiza Hina Ibraheem, Muhammad Rizwan Tariq, Shinawar Waseem Ali, Zujaja Umer, Zunaira Basharat, Azeem Intisar, Tariq Mahmood, Gulzar Ahmad Nayik, Seema Ramniwas, Saleh Alfarraj, Mohammad Javed Ansari

This research aimed to explore the influence of nutritional adjustments and storage stability on a functional, reduced-calorie guava jam incorporating Aloe vera. Over two months, comprehensive analysis assessed physicochemical properties, sensory traits, microbial stability, and shelf-life. The addition of Aloe vera gel resulted in significant improvements in pH (3.00 to 3.65), total soluble solids (40.10 to 42.20°Brix), antioxidant activity (36.85% to 81.09%), moisture content (29.48% to 38.82%), water activity (0.78 to 0.84), ash content (0.29% to 0.45%), fat content (0.14% to 0.19%), fibre content (1.05% to 1.86%), and the colour values. Moreover, b* scores for colour indication improved from 15.09 to 18.86. Texture attributes of cohesiveness and firmness improved significantly. Sensory evaluation favoured the T2 variant (20% Aloe vera gel), suggesting it as the optimal formulation. Furthermore, artificial neural networks (ANNs), a technique of machine learning, were utilised to predict guava jam behaviour, with 99% accuracy. The study discovered substantial changes in pH, total soluble solids, antioxidant activity, moisture content, and textural qualities, indicating that Aloe vera supplementation could improve guava jam quality and shelf-life. The results of ANN predictions about antioxidants and cohesiveness provide information about the product's performance during storage.

本研究旨在探索营养调整和储存稳定性对加入芦荟的功能性低热量番石榴果酱的影响。在两个月的时间里,对理化特性、感官特征、微生物稳定性和保质期进行了综合分析评估。添加芦荟胶后,pH 值(3.00 至 3.65)、总可溶性固形物(40.10 至 42.20°Brix)、抗氧化活性(36.85% 至 81.09%)、水分含量(29.48% 至 38.82%)、水分活性(0.78 至 0.84)、灰分含量(0.29% 至 0.45%)、脂肪含量(0.14% 至 0.19%)、纤维含量(1.05% 至 1.86%)和色值均有显著改善。此外,色泽指示的 b* 分数从 15.09 分提高到 18.86 分。质地属性中的粘稠度和紧实度也有明显改善。感官评价更倾向于 T2 变体(20% 的芦荟胶),这表明它是最佳配方。此外,利用机器学习技术--人工神经网络(ANN)来预测番石榴果酱的特性,准确率高达 99%。研究发现,番石榴果酱的 pH 值、总可溶性固形物、抗氧化活性、水分含量和质地都发生了很大变化,这表明添加芦荟可以改善番石榴果酱的质量和货架期。ANN 对抗氧化剂和粘稠度的预测结果提供了产品在贮藏期间的性能信息。
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引用次数: 0
Viewpoint: could cultivated meat's real route to mainstream markets be as hybrid food - why this makes sense? 观点:栽培肉进入主流市场的真正途径可能是混合食品--为什么这样做有意义?
IF 3.3 3区 农林科学 Q1 Engineering Pub Date : 2024-05-17 DOI: 10.1111/ijfs.17190
Jette F. Young, Niall W. G. Young

From beginnings as an esoteric research topic, cultivated meat (produced from cells originating from the meat producing animals) has developed with increasing pace from a research concept and is starting to break into restaurant markets in Singapore, USA, and Israel. However, there remains a significant journey in the global food ecosystem in order for it to reach mainstream retail. This viewpoint piece will probe how combining the best from plant-based meat alternatives and cultivated meat disciplines may end up addressing consumers desires, delivering on sustainability, palatability, nutritional balance and economic viability.

摘要培养肉(由产肉动物的细胞生产)从一开始只是一个深奥的研究课题,到现在已经从一个研究概念发展得越来越快,并开始打入新加坡、美国和以色列的餐饮市场。然而,要使其进入主流零售市场,在全球食品生态系统中还有很长的路要走。这篇观点文章将探讨如何将植物肉类替代品和栽培肉类学科的精华结合起来,最终满足消费者的愿望,实现可持续性、适口性、营养平衡和经济可行性。
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引用次数: 0
Characterisation of wholegrain composite for pretzels: a comprehensive nutritional and functional analysis 椒盐脆饼全麦复合材料的特性:营养和功能综合分析
IF 3.3 3区 农林科学 Q1 Engineering Pub Date : 2024-05-17 DOI: 10.1111/ijfs.17213
Sana Amin, Imran Pasha, Amina Amin, Farah Ahmed, Rana Muhammad Aadil

Wholegrain composite flours are used for bakery products to enhance their nutritional value and to decrease the reliance on wheat importation as well as to ensure food security in most developing countries. This work aimed to optimise oat, maize, barley and wheat flour levels in the development of nutritionally enriched wholegrain pretzels. Composite flour blends were prepared by mechanical mixing of oat flour (OF), barley flour (BF), maize flour (MF) and wheat flour (WF) in the following proportion: WF (100%) (T0 – control), W:B (90:10) (T1), W:M (90:10) (T2), W:O (90:10) (T3), W:B:M:O (85:5:5:5) (T4), W:B:M:O (76:8:8:8) (T5) and W:B:M:O (70:10:10:10) (T6). Wholegrain composite flour mixes were analysed for their proximate composition and antioxidant properties. The fat, protein, ash and moisture content significantly changed (P < 0.05) in all treatments, while the fibre content increased. DPPH radical scavenging activity and total phenolic and flavonoid content showed significant changes compared to the control. A rapid visco analyser determined multigrain flour's rheological and pasting properties, and the values showed significant (P < 0.05) differences for peak viscosity, final viscosity and pasting temperature. The final product was analysed for proximate analysis, texture, colour and sensory evaluation. The increase in moisture, protein, fibre and ash content of the product was observed. Moisture, fat and fibre content of pretzels exhibited significant correlations (P < 0.05) with the sensory parameters. A grouping of the samples in function of the chemical and sensory characteristics was depicted from the principal component analysis. Texture and colour analysis showed significant differences (P < 0.05). Sensory evaluation results showed that the overall score for acceptability found for composite flour mix which contains 10% barley, 10% oat and 10% maize with wheat in a 30:70 ratio was accepted by the consumer. Consumption of wholegrain pretzels as snack food can contribute to a nutritious and balanced diet.

在大多数发展中国家,全麦复合面粉被用于烘焙食品,以提高其营养价值,减少对小麦进口的依赖,并确保粮食安全。这项工作旨在优化燕麦、玉米、大麦和小麦粉的含量,以开发营养丰富的全麦椒盐脆饼。将燕麦粉(OF)、大麦粉(BF)、玉米粉(MF)和小麦粉(WF)按以下比例进行机械混合,制备复合面粉混合物:WF(100%)(T0 - 对照组)、W:B(90:10)(T1)、W:M(90:10)(T2)、W:O(90:10)(T3)、W:B:M:O(85:5:5:5)(T4)、W:B:M:O(76:8:8:8)(T5)和 W:B:M:O(70:10:10:10)(T6)。对全谷物复合面粉混合物的近似成分和抗氧化特性进行了分析。在所有处理中,脂肪、蛋白质、灰分和水分含量都发生了显著变化(P < 0.05),而纤维含量则有所增加。与对照组相比,DPPH 自由基清除活性、总酚和类黄酮含量均有明显变化。快速粘度分析仪测定了多谷物面粉的流变和糊化特性,结果表明,峰值粘度、最终粘度和糊化温度的数值差异显著(P < 0.05)。对最终产品进行了近似分析、质地、颜色和感官评价。观察到产品的水分、蛋白质、纤维和灰分含量都有所增加。椒盐脆饼的水分、脂肪和纤维含量与感官参数有显著的相关性(P < 0.05)。通过主成分分析,可以根据化学和感官特征对样品进行分组。质地和颜色分析表明差异显著(P < 0.05)。感官评价结果表明,消费者对含有 10%大麦、10%燕麦和 10%玉米与小麦的混合面粉(比例为 30:70)的可接受性总体得分较高。食用全麦椒盐脆饼作为小吃,有助于营养均衡的饮食。
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引用次数: 0
Effects of different extraction methods on the structure and function of blackberry seed polysaccharides 不同提取方法对黑莓种子多糖结构和功能的影响
IF 3.3 3区 农林科学 Q1 Engineering Pub Date : 2024-05-17 DOI: 10.1111/ijfs.17215
Mo Yang, Hao Gong, Cheng-gong Jiang, Yu He, Shuai Wang, Yi Zheng, Jin-hui Hou, Wei-dong Wang

The different extraction methods including hot-water extraction, ultrasound-assisted extraction, and microwave-assisted extraction, enzyme-assisted extraction, ultrasound and microwave-assisted extraction, and ultrasound and enzyme-assisted extraction (UEE) methods were carried out to extract blackberry seed polysaccharides (BSPs). The extraction yield, structure, antioxidant capacity, and glucometabolic enzyme inhibition activities of BSPs were determined in this work. Extraction methods have no influence on the monosaccharide composition of BSPs but affected the molar ratio of monosaccharides. Fourier transform infrared spectroscopy and X-ray diffraction results showed that the extraction method cannot effect on the functional groups and crystal forms of BSPs. The findings also revealed that UEE led to the highest BSP extraction yield (4.56%), highest antioxidant capacities, and highest α-amylase (48.49%) and α-glucosidase (67.33%) inhibitory rates, which may because of the dispersed molecular weight distribution and loose microstructure of BSPs. These results suggest that UEE may be used in the industry to obtain BSPs with high functional activities.

采用热水萃取、超声波辅助萃取、微波辅助萃取、酶辅助萃取、超声波和微波辅助萃取、超声波和酶辅助萃取(UEE)等不同的萃取方法提取黑莓种子多糖(BSPs)。该研究测定了黑莓籽多糖的提取率、结构、抗氧化能力和糖代谢酶抑制活性。提取方法对 BSP 的单糖组成没有影响,但对单糖的摩尔比有影响。傅立叶变换红外光谱和 X 射线衍射结果表明,提取方法不会影响 BSP 的官能团和晶体形态。研究结果还显示,UEE 的 BSP 提取率最高(4.56%),抗氧化能力最强,α-淀粉酶(48.49%)和α-葡萄糖苷酶(67.33%)抑制率最高,这可能与 BSP 分子量分布分散、微观结构疏松有关。这些结果表明,UEE 可用于工业领域,以获得具有高功能活性的 BSP。
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引用次数: 0
An alkali substrate feeding engineering conversion platform for hesperetin 7-O-glucoside biosynthesis 用于橙皮素 7-O-葡萄糖苷生物合成的碱底物喂养工程转化平台
IF 3.3 3区 农林科学 Q1 Engineering Pub Date : 2024-05-17 DOI: 10.1111/ijfs.17111
Chunqing Wang, Liang Chen, Zhendong Wang, Siming Zhu

The poor water solubility of flavonoids has always been a bottleneck restricting industrial production and application. The key problem of water-solubility of flavonoids was solved by establishing an alkali substrate-feeding engineering conversion platform using recombinant rhamnosidase. The results showed that the recombinant enzyme had α-L-rhamnosidase activity. Using this enzyme, four kinds of flavonoids such as hesperidin (HSD) were successfully hydrolyzed to products such as hesperetin 7-O-glucoside (HMG). Four key enzyme reaction conditions such as temperature, pH, feed rate and substrate specificity were investigated, and the optimum process parameters of the conversion platform were obtained. That was, under the conditions of temperature 50 °C, pH 6 and feeding speed 0.5 mL min−1, HSD conversion efficiency can reach 8.65 mg min−1. In addition, the changes in enzyme protein structure during basic feeding were elucidated by circular binary chromatography (CD) and fluorescence spectrum. The platform has the advantages of low cost, simple process, green environmental protection, high conversion rate and product purity. This method laid a solid foundation for the resource utilisation of flavonoid glycosides and industrial production.

黄酮类化合物的水溶性差一直是制约其工业化生产和应用的瓶颈。通过利用重组鼠李糖酶建立以碱为底物的工程转化平台,解决了黄酮类化合物水溶性的关键问题。结果表明,重组酶具有α-L-鼠李糖酶活性。利用这种酶,成功地将橙皮素(HSD)等四种黄酮类化合物水解为橙皮素 7-O-葡萄糖苷(HMG)等产物。研究了温度、pH值、进料速度和底物特异性等四个关键酶促反应条件,获得了转化平台的最佳工艺参数。即在温度 50 ℃、pH 6 和进料速度 0.5 mL min-1 的条件下,HSD 转化效率可达 8.65 mg min-1。此外,还通过循环二元色谱(CD)和荧光光谱阐明了基本进料过程中酶蛋白结构的变化。该平台具有成本低、工艺简单、绿色环保、转化率高、产品纯度高等优点。该方法为黄酮苷的资源化利用和工业化生产奠定了坚实的基础。
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引用次数: 0
期刊
International Journal of Food Science & Technology
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