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Molecular genetic diversity analysis using SSR markers of basmati rice (Oryza sativa L.) genotypes of northern hill region, India 利用SSR标记分析印度北部山区巴斯玛蒂稻基因型的分子遗传多样性
Pub Date : 2019-12-31 DOI: 10.25174/2249-4065/2019/95583
Aparajita Vatsa, R. Rathour, D. Basandrai, A. Sarial
© Society for Advancement of Wheat and Barley Research Abstract The genetic diversity of 30 rice accessions both of basmati and non-basmati including 2 red rices collected from Rice and Wheat Research Centre, Malan was performed on the basis of 36 genome wide SSR markers with the objectives to quantify the genetic divergence and to identify the potential accessions. Molecular characterization grouped them according to their varietal affiliations into five major clusters. Majority of basmati genotypes were clustered together. SSR markers detected a total of 83 alleles ranging from 2-4. The pairwise genetic similarity values among different genotypes ranged from 0.17 to 0.92 with an average of 0.48 indicating the presence of moderate genetic diversity among the genotypes analysed. The PIC values reflected the level of diversity and allelic frequency among the varieties which varied widely among SSR loci from 0.062 to 0.664. Two SSR markers namely, RM7173 and RM101 were identified as genotype specific, for genotype HPR 2761 and Vasumati, respectively. Two advanced breeding lines of basmati HPR 2852 and HPR 2855 were clustered together with their basmati parents in dendrogram indicating maximum recovery of basmati genotypes. The polymorphism detected among the genotypes will be helpful in selecting genetically diverse parents in the future breeding programme. The information generated will be used for advanced studies in germplasm, pureline selection and rice breeding in the future breeding programme.
摘要利用36个全基因组SSR标记,对来自马兰水稻和小麦研究中心的30份巴斯玛蒂和非巴斯玛蒂水稻材料(包括2份红水稻)的遗传多样性进行了分析,目的是量化遗传分化并鉴定潜在的材料。分子表征根据它们的品种隶属关系将它们分为五个主要集群。大多数巴斯马蒂基因型聚集在一起。SSR标记共检测到83个等位基因,范围为2 ~ 4。不同基因型间的遗传相似性值为0.17 ~ 0.92,平均为0.48,表明各基因型间存在中等程度的遗传多样性。PIC值反映了品种间的多样性水平和等位基因频率,在0.062 ~ 0.664之间差异较大。SSR标记RM7173和RM101分别对HPR 2761和Vasumati基因型具有基因型特异性。2个巴斯马蒂高级选育系HPR 2852和HPR 2855与其亲本在树状图上聚集在一起,表明巴斯马蒂基因型恢复最大。基因型间的多态性检测将有助于在今后的育种计划中选择遗传多样化的亲本。所产生的信息将用于未来育种计划中种质、纯种选择和水稻育种的高级研究。
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引用次数: 1
Effect of pre-mix pendimethalin + pyrazosulfuron on grain yield and yield attributes of rice (Oryza sativa L.) 戊二甲基灵+吡唑磺隆预混剂对水稻产量及产量性状的影响
Pub Date : 2019-12-31 DOI: 10.25174/2249-4065/2019/95896
T. Kaur, M. S. Bhullar, Fatehjeet Singh Sekhon
The experiment was carried out at Research Farm in Department of Agronomy, Punjab Agricultural University, Ludhiana to work out effective weed management strategy in transplanted rice. Nine treatments viz. pendimethalin + pyrazosulfuronat 675 + 15 g, 900 + 20 g, 1125 + 25 g, pendimethalin at 1500 g, pyrazosulfuronat 15 g, pyrazosulfuronat 20 g, metsulfuron + chlorimuronat 4 g ha -1 , hand weeding and weedy check replicated thrice laid out in randomized complete block design (RCBD). The results revealed that pendimethalin + pyrazosulfuron at 1125 + 25 g ha -1 , provided effective weed control (73.8-86.2% weed control efficiency) over other treatments. During 2015, pendimethalin + pyrazosulfuron at 1125 + 25 g ha -1 was found profitable with higher rice grain yield (6.65 t ha -1 ) and cost benefit ratio (1:2.25). Pendimethalin + pyrazosulfuronatat 900 + 20 g ha -1 , was found profitable with higher rice grain yield (7.35 t ha -1 ) and cost benefit ratio (1:2.50) and was statistically at par with pendimethalin + pyrazosulfuron at 1125 + 25 g ha -1 for weed control during 2016.
本试验在旁遮普省农业大学农学系研究农场进行,旨在制定插秧水稻有效的杂草管理策略。采用随机完全区组设计(RCBD)进行了手除草和杂草试验,9个处理分别为:二甲甲烷+吡唑磺隆675 + 15g、900 + 20g、1125 + 25g、二甲甲烷1500 g、吡唑磺隆15g、吡唑磺隆20g、甲磺隆+氯脲4g ha -1。结果表明,喷施1125 + 25 g ha -1的二甲甲烷+吡唑隆对杂草的防效为73.8% ~ 86.2%。2015年,使用1125 + 25 g - ha -1的二甲甲烷+吡唑隆可获得较高的水稻产量(6.65 t - ha -1)和成本效益比(1:2.25)。在2016年的杂草防治中,使用900 + 20 g - ha -1的二甲醚+吡唑磺隆具有较高的水稻产量(7.35 t - ha -1)和成本效益比(1:2.50),在统计上与使用1125 + 25 g - ha -1的二甲醚+吡唑磺隆相当。
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引用次数: 3
Comparative biology and population build-up of corn leaf aphid, R. maidis on barley genotypes 玉米叶蚜对大麦基因型的比较生物学及种群积累
Pub Date : 2019-09-03 DOI: 10.25174/2249-4065/2019/84867
Sandeep Singh, Beant Singh, J. Jindal
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引用次数: 0
Morpho-Physiological Diversity in Indian Spring Wheat cultivars and Identification of Promising Donor under Terminal Heat Stress 末热胁迫下印度春小麦品种形态生理多样性及潜在供体的鉴定
Pub Date : 2019-09-03 DOI: 10.25174/2249-4065/2019/89261
T. Singh, J. Kumari, R. Sharma, K. Shivani, Sunil Kumar, S. Jacob
© Society for Advancement of Wheat and Barley Research Abstract Assessment of genetic diversity and its application for wheat breeding resulted in enhanced and sustainable production and productivity. One hundred and two spring wheat cultivars were evaluated for 19 agro-morphological and physiological traits under late sown conditions for two years. The cluster analysis revealed significant genetic variability among wheat cultivars that shows the excellent opportunity to bring about improvement through hybridizing the selected genotypes present in distant clusters. The data was further subjected to PCA (principal component analysis) and genotype by trait biplot analysis. The first 2 principal components accounted for 33.8% of total variation. Phenological traits and growing degree days had major contribution towards heat tolerance as indicated by principal component analysis. Correlation analysis revealed that grain yield was positively associated with biological yield and harvest index. Promising cultivars were identified for terminal heat tolerance that can be adapted for growing under late sown condition and further used for breeding improved climate resilient wheat cultivars and genomics study.
©美国小麦和大麦研究进步学会摘要遗传多样性评估及其在小麦育种中的应用有助于提高和可持续的产量和生产力。对102个春小麦品种进行了2年晚播条件下19项农业形态和生理性状的评价。聚类分析显示,小麦品种间存在显著的遗传变异,表明通过杂交远缘聚类中所选基因型进行改良的绝佳机会。数据进一步进行主成分分析(PCA)和性状双图分析的基因型分析。前2主成分占总变异量的33.8%。主成分分析表明,物候性状和生长度数对耐热性的影响最大。相关分析表明,粮食产量与生物产量和收获指数呈正相关。鉴定出适合晚播条件下生长的终端耐热品种,并将其进一步用于小麦气候适应性改良品种选育和基因组学研究。
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引用次数: 1
Identification of yellow rust resistance sources in advanced breeding lines of barley (Hordeum vulgare L.) 大麦高级选育系抗黄锈源的鉴定
Pub Date : 2019-09-03 DOI: 10.25174/2249-4065/2019/85076
Jogendra Singh, O. P. Gangwar, P. S. Shekhawat, J. Kaur, Dinesh Kumar, V. Kumar, M. Pandey, R. Devlash, A. S. Kharub, G. Singh
1ICAR-Indian Institute of Wheat and Barley Research, Karnal-132 001, Haryana, India 2ICAR-Indian Institute of Wheat and Barley Research, Regional Station, Flowerdale, Shimla-171 002, Himachal Pradesh, India 3SKNAU-Rajasthan Agricultural Research Institute, Durgapura-302 018, Rajasthan, India 4Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141004, India 5SKUAST-J., Main Campus, Chatha, Jammu-180 009, J&K, India 6CSK HPKVV, Hill Agricultural Research and Extension Centre, Bajaura-175 125, Kulu, Himachal Pradesh, India
1 icar -印度小麦和大麦研究所,印度哈里亚纳邦卡纳-132 001;2 icar -印度小麦和大麦研究所,印度喜马偕尔邦希姆拉-171 002,花代尔区域站;3 sk瑙尔-拉贾斯坦邦农业研究所,杜尔加普拉-302 018,印度拉贾斯坦邦;4旁遮普农业大学植物育种与遗传学系,印度卢迪亚纳-141004;6CSK HPKVV,山地农业研究与推广中心,Bajaura-175 125,喜马偕尔邦库鲁,印度
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引用次数: 0
Assessment of heterosis and inbreeding depression for agro-morphological traits in bread wheat 面包小麦农业形态性状杂种优势及近交抑制评价
Pub Date : 2019-09-03 DOI: 10.25174/2249-4065/2019/89648
Pradeep Kumar, Satnam Singh Nagar, Charan Singh, Vikas Gupta, G. Singh, B. Tyagi
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引用次数: 0
Agro-morphological and Molecular Assessment of Advanced Wheat Breeding Lines for Grain Yield, Quality and Rust Resistance 小麦高级选品系产量、品质及抗锈性的农业形态与分子评价
Pub Date : 2019-09-03 DOI: 10.25174/2249-4065/2019/90162
Satish Kumar, G. Sandhu, S. Yadav, V. Pandey, O. Prakash, A. Verma, S. Bhardwaj, R. Chatrath, G. Singh
The genetic gain for yield in wheat and other cereals has been known to be stagnating in recent years. Breeding wheat cultivars with increased genetic potential can contribute to meet at least half of the desired production increases. In this context we have developed some advanced wheat lines in our breeding program, which aims to utilize the untapped variability. We assessed a set of 60 diverse lines along with four commercial check varieties for various agro-morphological traits, grain quality and yield based and also the adaptability using molecular markers. Significant genotypic differences were observed for most of the grain quality and agro-morphological traits recorded, including grain yield. We could identify lines which have significantly higher grain yield. The diversity analysis carried out using the DNA markers could explain the variability for different traits in this set. This set of genotypes is thus a very useful source as far as enhancing the yield levels. The lines developed through the conventional breeding program were based on crosses between elite breeding lines and specific donors and hence might resulted in higher yield potential.
众所周知,小麦和其他谷物产量的遗传增益近年来停滞不前。培育遗传潜力更高的小麦品种可以至少满足一半的预期产量增长。在这种情况下,我们在育种计划中开发了一些先进的小麦品系,目的是利用未开发的变异。我们利用分子标记对60个不同品系和4个商业对照品种的各种农业形态性状、籽粒品质和产量以及适应性进行了评估。籽粒品质和农业形态性状(包括产量)的基因型差异显著。我们可以识别出产量显著提高的品系。利用DNA标记进行的多样性分析可以解释该集合中不同性状的变异性。因此,就提高产量水平而言,这组基因型是非常有用的来源。通过传统育种计划开发的品系是基于精英育种品系与特定供体之间的杂交,因此可能产生更高的产量潜力。
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引用次数: 3
Hulless Barley: A new era of research for food purposes 无壳大麦:食品研究的新时代
Pub Date : 2019-09-03 DOI: 10.25174/2249-4065/2019/83719
H. Kaur, Shaveta Shaveta, S. Kaur, V. Sharma, K. Kaur
Barley (Hordeum vulgare L.) is included in the family of grasses and is the fourth highly significant crop after maize, wheat and rice. Barley is considered as an important food ingredient because of the presence of vital biochemical constituents viz. β-glucan, starch, amylose, protein etc. Barley is usually classified as hulless or hulled types (absence or presence of hull adhering to grain). Hulless barley needs negligible processing and preserves maximum of the endosperm and germ, which is once in a while lost during the time spent for dehulling. As a result, it is highly suitable for human utilization, as the entire grain can be specifically utilized to form a meal or processed into flour. Interest in the use of hulless barley as a food grain has expanded primarily due to its stated health benefits .The regular intake of hulless barley contributes to maintenance of normal blood cholesterol level, beneficial in preventing certain types of cancer like colon cancer and also provides better glucose and insulin responses. Hulless barley has high β-glucan content which helps in lowering of glycemic index and also causes the stimulation of bowel health. For this reason food industry is anxious about expanding the utilization of these cereals as nourishment fixings and henceforth more research is merited here.
大麦(Hordeum vulgare L.)属于禾本科植物,是继玉米、小麦和水稻之后的第四大重要作物。大麦被认为是一种重要的食品原料,因为它含有重要的生化成分,如β-葡聚糖、淀粉、直链淀粉、蛋白质等。大麦通常分为无壳型和有壳型(无壳或有壳附着在谷物上)。去壳大麦的加工过程可以忽略不计,它能最大限度地保存胚乳和胚芽,而胚乳和胚芽在去壳过程中偶尔会丢失。因此,它非常适合人类利用,因为整个谷物可以专门用来形成膳食或加工成面粉。人们对使用无壳大麦作为食物谷物的兴趣越来越大,主要是因为它对健康有好处。经常摄入无壳大麦有助于维持正常的血液胆固醇水平,有助于预防某些类型的癌症,如结肠癌,还能提供更好的葡萄糖和胰岛素反应。无壳大麦含有高β-葡聚糖,有助于降低血糖指数,也能促进肠道健康。因此,食品工业急于扩大这些谷物作为营养辅料的利用,因此值得在这方面进行更多的研究。
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引用次数: 17
Estimation of genetic parameters and heterosis in cytoplasmic male sterility based experimental hybrids for yield and component traits 细胞质雄性不育试验杂交种产量和组成性状的遗传参数和杂种优势估计
Pub Date : 2019-09-03 DOI: 10.25174/2249-4065/2019/82125
S. Singh, R. Singh, Snehanshu Singh, Amita Sharma
Development of hybrids in wheat is an innovative approach for enhancing present productivity levels. Presence of standard or commercial heterosis is essential for successful development of a hybrid cultivar. The discovery of heterosis and its exploitation in modern plant breeding programmes is one of the most important advances in plant improvement (Alghamdi, 2009). Although hybrid development in wheat could not achieve any success in the world but efforts are being made for development of CMS (Cytoplasmic Male Sterility) based hybrids in wheat for marginal land with half seed rate and in this direction, more than 50 diversified cytoplasmic male sterile (CMS) lines were developed in agronomic background of Indian wheat cultivars using backcross breeding at ICAR-IIWBR, Karnal (Singh et al., 2016). These new CMS lines were evaluated for male sterility and 12 CMS lines in agronomic background of DBW 17 (a promising cultivar of NWPZ were crossed with 3 fertility restorer lines (Res 5, Res 37 & Res 38) during 2016-17 crop season to develop 36 experimental hybrids. These CMS based 36 experimental hybrids Homepage: http://epubs.icar.org.in/ejournal/index.php/JWR
开发小麦杂交品种是提高当前生产力水平的一种创新途径。标准或商业杂种优势的存在对杂交品种的成功发展至关重要。杂种优势的发现及其在现代植物育种计划中的利用是植物改良中最重要的进展之一(Alghamdi, 2009)。尽管小麦的杂交开发在世界上没有取得任何成功,但人们正在努力开发基于CMS(细胞质雄性不育)的小麦杂交品种,用于边际土地,种子率为一半,在这个方向上,在印度小麦栽培品种的农艺背景下,利用ICAR-IIWBR, Karnal的回交育种,开发了50多个多样化的细胞质雄性不育(CMS)系(Singh等,2016)。在2016- 2017作物季,对这些新不育系进行了雄性不育性评价,并在新水稻的农艺背景下,与3个育性恢复系(瑞5号、瑞37号和瑞38号)杂交,获得了36个试验杂交种。这些基于CMS的36个实验性杂交品种主页:http://epubs.icar.org.in/ejournal/index.php/JWR
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引用次数: 1
Management of spot blotch of wheat using inducer chemicals under field conditions 田间条件下诱导剂对小麦斑病的治理
Pub Date : 2019-09-03 DOI: 10.25174/2249-4065/2019/86740
Meronbala Hijam, S. Mahapatra, Srikanta Das
An experiment was conducted for two consecutive years (2012-13 and 2013-14) to determine the effect of different inducer chemicals on spot blotch disease of wheat and its impact on grain yield. All the inducer chemicals reduced the spot blotch infection significantly at different concentration levelsand also increase the yield attributing characters like number of grains/panicle and 1000 seed weight. The accumulation of phenol increased up to 96 days after sowing (DAS), whereas, peroxidase accumulation increased up to 68 days after sowing in wheat plants in all the treatments.Among the different inducer chemicals salicyclic acid (10 -4 M) and CuSO4 (10 -4 M and 10 -5 M) showed good results even at low concentration levels. Therefore, the inducer chemicals can be used as an alternative method to manage the spot blotch of wheat.
本试验连续两年(2012-13和2013-14)研究了不同诱导剂对小麦斑病的防治效果及其对籽粒产量的影响。各诱导剂在不同浓度水平下均显著降低了斑病的侵染率,并提高了籽粒/穗数和千粒重等产量性状。各处理小麦植株中苯酚积累量增加至播后96 d,而过氧化物酶积累量增加至播后68 d。在不同的诱导剂中,水杨酸(10 -4 M)和CuSO4 (10 -4 M和10 -5 M)在低浓度下也表现出良好的诱导剂效果。因此,诱导剂可作为小麦斑病防治的一种替代方法。
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引用次数: 8
期刊
Wheat and Barley Research
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