Pub Date : 2024-10-01Epub Date: 2024-10-30DOI: 10.15386/mpr-2777
Raluca Andrada Munteanu, Cristian Silviu Moldovan, Adrian Bogdan Tigu, Rares Drula, Richard Feder, Lorand Magdo, Ancuta Jurj, Lajos Raduly, Liviuta Budisan, Radu Pirlog, Alin Moldovan, Alina-Andreea Zimta, Cornelia Braicu, Alexandra Preda, Vlad Munteanu, Mihai Romitan, Diana Gulei, Tudor Eliade Ciuleanu
Background and aims: Non-small cell lung cancer (NSCLC) treatment is challenged by late detection and limited therapeutic options. Aberrant DNA methylation, a common epigenetic alteration in NSCLC, offers new therapeutic avenues. This study aims to evaluate the combined effects of 5-Azacytidine (5-Aza), an epigenetic modifier, and ionizing radiation (IR) on NSCLC, exploring the underlying molecular mechanisms and therapeutic potential.
Methods: In this study, we examined the effects of 5-Aza combined with IR in both in vitro and in vivo models of NSCLC. Five human NSCLC cell lines were treated with 5-Aza and IR. Cell viability, colony formation, wound healing, and transwell migration assays were performed to assess treatment effects. Microarray and qPCR analyses were conducted to identify gene expression changes. Additionally, subcutaneous and orthotopic xenograft models were used to evaluate the treatment's efficacy in vivo.
Results: Treatment with 5-Aza and IR resulted in significant reductions in cell viability, colony formation, and migration in NSCLC cell lines. Microarray analysis revealed significant changes in gene expression, including the upregulation of apoptosis-related genes and the downregulation of cell proliferation-related genes. In vivo studies demonstrated a notable reduction in tumor growth and metastasis in both subcutaneous and orthotopic NSCLC models following 5-Aza and IR treatment. Histological and bioluminescent imaging confirmed the therapeutic effects of the combined treatment.
Conclusions: The combination of 5-Aza and IR shows promise as an effective treatment for NSCLC, enhancing apoptosis and reducing tumor growth through epigenetic modulation.
背景和目的：非小细胞肺癌（NSCLC）的治疗面临着发现较晚和治疗方案有限的挑战。DNA甲基化异常是非小细胞肺癌常见的表观遗传学改变，它提供了新的治疗途径。本研究旨在评估表观遗传修饰剂 5-氮杂胞苷（5-Aza）和电离辐射（IR）对 NSCLC 的联合作用，探索其潜在的分子机制和治疗潜力：在这项研究中，我们考察了 5-Aza 与 IR 在 NSCLC 体外和体内模型中的联合作用。五种人类 NSCLC 细胞系接受了 5-Aza 和 IR 的治疗。为了评估治疗效果，我们进行了细胞活力、集落形成、伤口愈合和跨孔迁移试验。进行芯片和 qPCR 分析以确定基因表达的变化。此外，还使用皮下和正位异种移植模型来评估治疗在体内的疗效：结果：5-Aza和IR可显著降低NSCLC细胞系的细胞活力、集落形成和迁移。芯片分析显示基因表达发生了重大变化，包括凋亡相关基因的上调和细胞增殖相关基因的下调。体内研究表明，5-Aza 和 IR 治疗后，皮下和正位 NSCLC 模型中的肿瘤生长和转移明显减少。组织学和生物发光成像证实了联合治疗的疗效：结论：5-Aza 和 IR 的联合疗法有望成为一种有效的 NSCLC 治疗方法，通过表观遗传调控增强细胞凋亡并减少肿瘤生长。
{"title":"5-Azacytidine treatment inhibits the development of lung cancer models via epigenetic reprogramming and activation of cellular pathways with anti-tumor activity.","authors":"Raluca Andrada Munteanu, Cristian Silviu Moldovan, Adrian Bogdan Tigu, Rares Drula, Richard Feder, Lorand Magdo, Ancuta Jurj, Lajos Raduly, Liviuta Budisan, Radu Pirlog, Alin Moldovan, Alina-Andreea Zimta, Cornelia Braicu, Alexandra Preda, Vlad Munteanu, Mihai Romitan, Diana Gulei, Tudor Eliade Ciuleanu","doi":"10.15386/mpr-2777","DOIUrl":"10.15386/mpr-2777","url":null,"abstract":"<p><strong>Background and aims: </strong>Non-small cell lung cancer (NSCLC) treatment is challenged by late detection and limited therapeutic options. Aberrant DNA methylation, a common epigenetic alteration in NSCLC, offers new therapeutic avenues. This study aims to evaluate the combined effects of 5-Azacytidine (5-Aza), an epigenetic modifier, and ionizing radiation (IR) on NSCLC, exploring the underlying molecular mechanisms and therapeutic potential.</p><p><strong>Methods: </strong>In this study, we examined the effects of 5-Aza combined with IR in both <i>in vitro</i> and <i>in vivo</i> models of NSCLC. Five human NSCLC cell lines were treated with 5-Aza and IR. Cell viability, colony formation, wound healing, and transwell migration assays were performed to assess treatment effects. Microarray and qPCR analyses were conducted to identify gene expression changes. Additionally, subcutaneous and orthotopic xenograft models were used to evaluate the treatment's efficacy <i>in vivo</i>.</p><p><strong>Results: </strong>Treatment with 5-Aza and IR resulted in significant reductions in cell viability, colony formation, and migration in NSCLC cell lines. Microarray analysis revealed significant changes in gene expression, including the upregulation of apoptosis-related genes and the downregulation of cell proliferation-related genes. <i>In vivo</i> studies demonstrated a notable reduction in tumor growth and metastasis in both subcutaneous and orthotopic NSCLC models following 5-Aza and IR treatment. Histological and bioluminescent imaging confirmed the therapeutic effects of the combined treatment.</p><p><strong>Conclusions: </strong>The combination of 5-Aza and IR shows promise as an effective treatment for NSCLC, enhancing apoptosis and reducing tumor growth through epigenetic modulation.</p>","PeriodicalId":18438,"journal":{"name":"Medicine and Pharmacy Reports","volume":"97 4","pages":"488-506"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and aims: The non-dipper pattern is present in about 50 percent of patients with type 2 diabetes (T2D) and hypertension, a status associated with more frequent cardiovascular complications and restrained prognosis. This study aimed to identify simple biomarkers that can be used for the classification of dipper and non-dipper individuals with type 2 diabetes and hypertension.
Method: 138 consecutive patients with type 2 diabetes mellitus (DM) and hypertension underwent 24-hour ambulatory blood pressure monitoring (ABPM), 54 (39.1%) dippers and 84 (60.9%) non-dippers; CBC and determinations of different biomarkers, as well as their ratio was also performed, for comparing the two dipper profiles. The different dipper profiles were established by ABPM, which highlights the mean arterial pressure (MAP), the mean heart rate (MHR), and the mean pulse pressure (MPP). The area under the receiver operating characteristic curve (AUC) was used to evaluate the ability of biomarkers to differentiate dippers from non-dippers.
Results: The study included 54 dipper and 84 non-dipper patients. The median age was 64 years (interquartile range 58-74), ranging from 24 to 85 years. The comparison between dipper and non-dipper in patients with type 2 diabetes and hypertension concerning different biomarkers found only two that were statistically significant: triglycerides to hemoglobin A1c (HbA1c) ratio and triglycerides to glucose ratio. For both biomarkers, the dippers had higher values than non-dippers. The best AUCs were found for triglycerides (Trig) to glucose ratio of 0.774 (95% confidence interval 0.601 - 0.92), statistically significant, followed at a distance by lymphocytes, platelets-lymphocytes ratio (PLR), platelet distribution width (PDW-SD), mean platelet volume (MPV) /Lymphocytes, and others, none of them being statistically significant.
Conclusions: This study offers valuable insights into the classification of dipper and non-dipper individuals with type 2 diabetes and hypertension using several biomarkers. Notably, the triglyceride-to-glucose ratio appeared as a significant marker with considerable discriminative capacity, indicating its potential therapeutic value in risk stratification and personalized treatment strategies.
{"title":"The biomarkers associated with non-dipper pattern in patients with type 2 diabetes with hypertension.","authors":"Viorel Manea, Daniel-Corneliu Leucuţa, Călin Pop, Mircea-Ioachim Popescu","doi":"10.15386/mpr-2796","DOIUrl":"10.15386/mpr-2796","url":null,"abstract":"<p><strong>Background and aims: </strong>The non-dipper pattern is present in about 50 percent of patients with type 2 diabetes (T2D) and hypertension, a status associated with more frequent cardiovascular complications and restrained prognosis. This study aimed to identify simple biomarkers that can be used for the classification of dipper and non-dipper individuals with type 2 diabetes and hypertension.</p><p><strong>Method: </strong>138 consecutive patients with type 2 diabetes mellitus (DM) and hypertension underwent 24-hour ambulatory blood pressure monitoring (ABPM), 54 (39.1%) dippers and 84 (60.9%) non-dippers; CBC and determinations of different biomarkers, as well as their ratio was also performed, for comparing the two dipper profiles. The different dipper profiles were established by ABPM, which highlights the mean arterial pressure (MAP), the mean heart rate (MHR), and the mean pulse pressure (MPP). The area under the receiver operating characteristic curve (AUC) was used to evaluate the ability of biomarkers to differentiate dippers from non-dippers.</p><p><strong>Results: </strong>The study included 54 dipper and 84 non-dipper patients. The median age was 64 years (interquartile range 58-74), ranging from 24 to 85 years. The comparison between dipper and non-dipper in patients with type 2 diabetes and hypertension concerning different biomarkers found only two that were statistically significant: triglycerides to hemoglobin A1c (HbA1c) ratio and triglycerides to glucose ratio. For both biomarkers, the dippers had higher values than non-dippers. The best AUCs were found for triglycerides (Trig) to glucose ratio of 0.774 (95% confidence interval 0.601 - 0.92), statistically significant, followed at a distance by lymphocytes, platelets-lymphocytes ratio (PLR), platelet distribution width (PDW-SD), mean platelet volume (MPV) /Lymphocytes, and others, none of them being statistically significant.</p><p><strong>Conclusions: </strong>This study offers valuable insights into the classification of dipper and non-dipper individuals with type 2 diabetes and hypertension using several biomarkers. Notably, the triglyceride-to-glucose ratio appeared as a significant marker with considerable discriminative capacity, indicating its potential therapeutic value in risk stratification and personalized treatment strategies.</p>","PeriodicalId":18438,"journal":{"name":"Medicine and Pharmacy Reports","volume":"97 4","pages":"446-455"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01Epub Date: 2024-10-30DOI: 10.15386/mpr-2747
Florina Borozan, Alexandra Denisa Semenescu, Ioan Sas, Elena Bernad, Andrada Iftode, Claudia Iftode, Monica Susan, Daniela Radu
Background and aims: Adenomyosis is a heterogeneous disease, which differs from patient to patient. The objective of our study was to evaluate the risk factors that influence the occurrence of adenomyosis, more precisely to highlight aspects that may be used in practice. In addition, the in vitro impact of levonorgestrel (a possible predisposing factor in the occurrence of adenomyosis) on MDA-MB-231 cells was evaluated, trying to obtain a link between adenomyosis and mammary cancer.
Methods: Clinical and demographic data of patients diagnosed with adenomyosis hospitalized between January and September 2023 in the Obstetrics-Gynecology Clinic were analyzed. For the in vitro assays, the MTT and LDH method was used to investigate the effect on cell viability and the potential cytotoxic effect of LG on MDA-MB-23 cells.
Results: Out of a total of 99 hysterectomies performed, the diagnosis of adenomyosis was confirmed by ultrasound in 28 cases. Among our patients, we could observe that most of cases of adenomyosis developed in women between 40 and 45 years old. Multiple pregnancies can influence the development of this uterine pathology, along with a history of uterine surgery and abortions. It was also found that treatment with sex hormones can increase the risk of adenomyosis. Our in vitro study has showed that LG stimulates the proliferation of MDA-MB-231 cells depending on the dose and time.
Conclusions: Personal history along with progestin treatment may influence myometrial lesions, leading to diffuse or focal adenomyosis. Moreover, in vitro, LG has been shown to stimulate the proliferation of breast cancer cells.
{"title":"Retrospective study of the factors involved in the development of adenomyosis and the <i>in vitro</i> link between adenomyosis and breast cancer.","authors":"Florina Borozan, Alexandra Denisa Semenescu, Ioan Sas, Elena Bernad, Andrada Iftode, Claudia Iftode, Monica Susan, Daniela Radu","doi":"10.15386/mpr-2747","DOIUrl":"10.15386/mpr-2747","url":null,"abstract":"<p><strong>Background and aims: </strong>Adenomyosis is a heterogeneous disease, which differs from patient to patient. The objective of our study was to evaluate the risk factors that influence the occurrence of adenomyosis, more precisely to highlight aspects that may be used in practice. In addition, the <i>in vitro</i> impact of levonorgestrel (a possible predisposing factor in the occurrence of adenomyosis) on MDA-MB-231 cells was evaluated, trying to obtain a link between adenomyosis and mammary cancer.</p><p><strong>Methods: </strong>Clinical and demographic data of patients diagnosed with adenomyosis hospitalized between January and September 2023 in the Obstetrics-Gynecology Clinic were analyzed. For the <i>in vitro</i> assays, the MTT and LDH method was used to investigate the effect on cell viability and the potential cytotoxic effect of LG on MDA-MB-23 cells.</p><p><strong>Results: </strong>Out of a total of 99 hysterectomies performed, the diagnosis of adenomyosis was confirmed by ultrasound in 28 cases. Among our patients, we could observe that most of cases of adenomyosis developed in women between 40 and 45 years old. Multiple pregnancies can influence the development of this uterine pathology, along with a history of uterine surgery and abortions. It was also found that treatment with sex hormones can increase the risk of adenomyosis. Our <i>in vitro</i> study has showed that LG stimulates the proliferation of MDA-MB-231 cells depending on the dose and time.</p><p><strong>Conclusions: </strong>Personal history along with progestin treatment may influence myometrial lesions, leading to diffuse or focal adenomyosis. Moreover, <i>in vitro</i>, LG has been shown to stimulate the proliferation of breast cancer cells.</p>","PeriodicalId":18438,"journal":{"name":"Medicine and Pharmacy Reports","volume":"97 4","pages":"477-487"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cornelia Liana Micula Cociuban, Teodor Traian Maghiar, I. Olariu, E. Vasca, Adelina Ramona Berari, Ciprian Pasca, Razvan Flueras, R. Popovici, A. Matichescu, Diana Marian
Background and aims. The development of dental implantology is based on a thorough examination of the interaction of implants with surrounding tissues, as well as methods of stimulating osteogenesis around implants. Consequently, the most common approach to restore lost function in terms of function and aesthetics is now treating partially edentulous individuals with implants. The study's objective was to assess how effectively implant prosthetic treatments performed only on implants reconstruction and on implants in conjunction with abutment teeth. Methods. A total of seven years (2016–2023) were dedicated to this study, with evaluation at one, two, three, and five years. For this analysis, MedCalc® version 12.5.0.0 (MedCalc® Software, Mariakerke, Belgium) was utilised as the medical statistics software. Results and conclusions. When using dental implants and natural teeth abutments for prosthodontic reconstruction, the failure rates rise approximately 43 times when compared to dental implant rehabilitation; similarly, osteoporosis and diabetes mellitus increase failure rates by 32 and 20 times, respectively. Gingival inflammation is a frequent event (almost 50% frequency) observed during follow-up in patients who had prosthetic restoration using dental implants alone as well as implants and abutment teeth. For both groups, difficulties usually arise two years later.
{"title":"Clinical-statistical study on oral rehabilitation methods using dental implants","authors":"Cornelia Liana Micula Cociuban, Teodor Traian Maghiar, I. Olariu, E. Vasca, Adelina Ramona Berari, Ciprian Pasca, Razvan Flueras, R. Popovici, A. Matichescu, Diana Marian","doi":"10.15386/mpr-2772","DOIUrl":"https://doi.org/10.15386/mpr-2772","url":null,"abstract":"Background and aims. The development of dental implantology is based on a thorough examination of the interaction of implants with surrounding tissues, as well as methods of stimulating osteogenesis around implants. Consequently, the most common approach to restore lost function in terms of function and aesthetics is now treating partially edentulous individuals with implants. The study's objective was to assess how effectively implant prosthetic treatments performed only on implants reconstruction and on implants in conjunction with abutment teeth. \u0000Methods. A total of seven years (2016–2023) were dedicated to this study, with evaluation at one, two, three, and five years. For this analysis, MedCalc® version 12.5.0.0 (MedCalc® Software, Mariakerke, Belgium) was utilised as the medical statistics software. \u0000Results and conclusions. When using dental implants and natural teeth abutments for prosthodontic reconstruction, the failure rates rise approximately 43 times when compared to dental implant rehabilitation; similarly, osteoporosis and diabetes mellitus increase failure rates by 32 and 20 times, respectively. Gingival inflammation is a frequent event (almost 50% frequency) observed during follow-up in patients who had prosthetic restoration using dental implants alone as well as implants and abutment teeth. For both groups, difficulties usually arise two years later.","PeriodicalId":18438,"journal":{"name":"Medicine and Pharmacy Reports","volume":"39 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141807699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Bîrsan, A. Cristofor, Cristina Tuchiluș, F. Crivoi, R. Vlad, Cezara Pintea, P. Antonoaea, A. Ciurba
Background and aims. The individualization of cosmetic products or personalized dermatology preparations are in great demand at the present time. Methods. 24 emulsifying cream bases were proposed which were prepared by the classical, automatic and semi-automatic methods, respectively, and the physical stability resulted from the three types of homogenization was taken into account. Texture parameters were also studied for the most stable cream bases in the preformulation stage and the t - statistical test was applied. In order to choose the most optimal preservative, the effectiveness of the NipaEster solution 0.1%, Cosgard and Euxyl® PE 9010 was tested on the strains of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. Results. 9 cream bases were stable through all the preparation methods used, and preservation was achieved with Euxyl® PE 9010. Following the texture parameters, significant differences were observed for the same formula in the case of choosing a different preparation method. Conclusions. Formulas F1, with methyl glucose sesquistearate as emulsifier, F8, with cetearyl glucosite as emulsifier, and F14, with Ceteareth-20 can be used as cream bases for customized products.
背景和目的。目前，个性化美容产品或个性化皮肤科制剂的需求量很大。研究方法提出了 24 种乳化膏基，分别采用传统、自动和半自动方法制备，并考虑了三种均质化方式所产生的物理稳定性。此外，还对预配制阶段最稳定的乳脂基质的质地参数进行了研究，并采用了 t 统计检验。为了选择最理想的防腐剂，测试了 NipaEster 溶液 0.1%、Cosgard 和 Euxyl® PE 9010 对金黄色葡萄球菌、绿脓杆菌和白色念珠菌的功效。结果显示在所有使用的制备方法中，9 种膏霜基质都很稳定，而 Euxyl® PE 9010 则达到了防腐效果。根据质地参数，在选择不同配制方法的情况下，相同配方的质地有显著差异。结论以甲基葡萄糖倍半硬脂酸酯为乳化剂的配方 F1、以鲸蜡硬脂醇葡糖酸酯为乳化剂的配方 F8 和以鲸蜡硬脂醇聚醚-20 为乳化剂的配方 F14 可用作定制产品的膏霜基。
{"title":"Development of cream bases suitable for personalized cosmetic products","authors":"M. Bîrsan, A. Cristofor, Cristina Tuchiluș, F. Crivoi, R. Vlad, Cezara Pintea, P. Antonoaea, A. Ciurba","doi":"10.15386/mpr-2765","DOIUrl":"https://doi.org/10.15386/mpr-2765","url":null,"abstract":"Background and aims. The individualization of cosmetic products or personalized dermatology preparations are in great demand at the present time. \u0000Methods. 24 emulsifying cream bases were proposed which were prepared by the classical, automatic and semi-automatic methods, respectively, and the physical stability resulted from the three types of homogenization was taken into account. Texture parameters were also studied for the most stable cream bases in the preformulation stage and the t - statistical test was applied. In order to choose the most optimal preservative, the effectiveness of the NipaEster solution 0.1%, Cosgard and Euxyl® PE 9010 was tested on the strains of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. \u0000Results. 9 cream bases were stable through all the preparation methods used, and preservation was achieved with Euxyl® PE 9010. Following the texture parameters, significant differences were observed for the same formula in the case of choosing a different preparation method. \u0000Conclusions. Formulas F1, with methyl glucose sesquistearate as emulsifier, F8, with cetearyl glucosite as emulsifier, and F14, with Ceteareth-20 can be used as cream bases for customized products.","PeriodicalId":18438,"journal":{"name":"Medicine and Pharmacy Reports","volume":"50 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141809689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and aims. Vitamin D deficiency is widespread across the globe. Numerous reports have linked vitamin D deficiency to certain non-skeletal diseases such as cardiovascular diseases. According to recent studies, there is evidence indicating a possible link between 25-hydroxyvitamin D deficiency and dyslipidemia. The main aim of this study is to investigate the relationship between vitamin D levels and lipid profile and to identify people who may benefit from vitamin D supplementation. Methods. In this observational study, a total of 154 patients were included, 98 women and 56 men, aged between 19 and 82 years, in which serum vitamin D levels, total cholesterol, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), triglycerides (TG) and blood sugar were analyzed. Results. The serum levels of vitamin D showed some differences, being lower in patients with dyslipidemia, with a positive correlation between vitamin D levels and total cholesterol (F ratio = 7.3247, p=0.008), and also with LDL cholesterol (F ratio = 5.0023, p=0.027). The HDL-C fraction and triglycerides showed no significant correlation with the serum levels of vitamin D. Further on, we divided the patients according to the fraction that had pathological values and compared the levels of vitamin D between these categories. We observed that the lowest levels of vitamin D were present in patients with all lipid parameters modified (HIGH-TC/ LOW-HDL/HIGH-LDL/HIGH-TG), and also the highest levels of low HDL-C and high LDL-C. Conclusion. Our research provides additional evidence to the unfavorable lipid profile found in people with vitamin D deficiency.
背景和目的。维生素 D 缺乏症在全球普遍存在。大量报告显示，维生素 D 缺乏与某些非骨骼疾病（如心血管疾病）有关。根据最近的研究，有证据表明 25- 羟基维生素 D 缺乏症与血脂异常之间可能存在联系。本研究的主要目的是调查维生素 D 水平与血脂状况之间的关系，并找出可能从补充维生素 D 中受益的人群。研究方法在这项观察性研究中，共纳入了 154 名患者，其中女性 98 人，男性 56 人，年龄在 19 岁至 82 岁之间，分析了血清维生素 D 水平、总胆固醇、高密度脂蛋白胆固醇 (HDL-C)、低密度脂蛋白胆固醇 (LDL-C)、甘油三酯 (TG) 和血糖。结果显示血清中的维生素 D 水平显示出一些差异，血脂异常患者的维生素 D 水平较低，维生素 D 水平与总胆固醇呈正相关（F ratio = 7.3247，p=0.008），与低密度脂蛋白胆固醇也呈正相关（F ratio = 5.0023，p=0.027）。高密度脂蛋白胆固醇部分和甘油三酯与血清中的维生素 D 水平没有明显的相关性。此外，我们还根据病理值的部分对患者进行了划分，并比较了这些类别之间的维生素 D 水平。我们发现，所有血脂参数都发生变化（高-TC/低-HDL/高-LDL/高-TG）的患者维生素 D 水平最低，低 HDL-C 和高 LDL-C 水平也最高。结论我们的研究为维生素 D 缺乏症患者的不良血脂状况提供了更多证据。
{"title":"Association between vitamin D deficiency and serum lipid levels in a group of Romanian patients","authors":"C. Frențușcă, K. Babes","doi":"10.15386/mpr-2714","DOIUrl":"https://doi.org/10.15386/mpr-2714","url":null,"abstract":"Background and aims. Vitamin D deficiency is widespread across the globe. Numerous reports have linked vitamin D deficiency to certain non-skeletal diseases such as cardiovascular diseases. According to recent studies, there is evidence indicating a possible link between 25-hydroxyvitamin D deficiency and dyslipidemia. The main aim of this study is to investigate the relationship between vitamin D levels and lipid profile and to identify people who may benefit from vitamin D supplementation. \u0000Methods. In this observational study, a total of 154 patients were included, 98 women and 56 men, aged between 19 and 82 years, in which serum vitamin D levels, total cholesterol, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), triglycerides (TG) and blood sugar were analyzed. \u0000Results. The serum levels of vitamin D showed some differences, being lower in patients with dyslipidemia, with a positive correlation between vitamin D levels and total cholesterol (F ratio = 7.3247, p=0.008), and also with LDL cholesterol (F ratio = 5.0023, p=0.027). The HDL-C fraction and triglycerides showed no significant correlation with the serum levels of vitamin D. Further on, we divided the patients according to the fraction that had pathological values and compared the levels of vitamin D between these categories. We observed that the lowest levels of vitamin D were present in patients with all lipid parameters modified (HIGH-TC/ LOW-HDL/HIGH-LDL/HIGH-TG), and also the highest levels of low HDL-C and high LDL-C. \u0000Conclusion. Our research provides additional evidence to the unfavorable lipid profile found in people with vitamin D deficiency.","PeriodicalId":18438,"journal":{"name":"Medicine and Pharmacy Reports","volume":"65 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141808210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cosmin Ionuț Lixandru, Ionela Maniu, Mihaela Cernuşcă-Miţariu, C. Domnariu
Background and aims. The present study was carried out in the Department of Oral and Maxillo-Facial Surgery of the Sibiu County Emergency Clinical Hospital and aimed to evaluate the oral health status of patients who refer to this medical unit, identifying the presence and frequency of dental infections among these patients. The study also aimed to identify and characterize the comorbidities present in patients with these oral conditions. Finally, the study aims to highlight the degree of concern regarding the oral health status of patients and provide relevant information for dental health services and for the development of appropriate prevention and intervention strategies. Methods. The study was conducted retrospectively and descriptively, using the hospital’s electronic medical records. The total sample consisted of 1246 people, the inclusion criteria were: (a) patients admitted to the OMF Surgery Department; (b) outpatients who required hospitalization; (c) patients hospitalized under continuous hospitalization regime; (d) patients over 16 years of age; (e) patients diagnosed with infectious pathologies. After data registration, information on sex, background, distribution of diagnoses, associated pathologies of the patients and the existence of combined pathologies of the patients included in the study were taken into account. The duration of hospitalization of patients, the need and duration of antibiotic therapy, the frequency of use of combinations of antibiotics, and the number of antibiotics used in correlation with the duration of hospitalization were also analyzed. The data are presented as frequency and percentages. The combination of the most frequent associated pathologies was analyzed using an algorithm based on association rules and chord diagram was used for their visual representation. Statistical analyses were performed using IBM SPSS® (Statistical Package for the Social Science) version 20 and R software. Results. The total sample consisted of a total of 1246 people. From the preliminary analysis, the majority of people in the sample, 68.5%, did not suffer from infectious conditions, while 31.5% were affected. Most of the patients came from the urban environment (61.73%) and were male (54.34%). Frequent comorbidities included cardiac pathologies (11.99%) and diabetes (4.08%). These data indicate the poor state of oral health among patients who call on the OMF Surgery services. It is not an isolated case, but it reflects a pattern observed globally. Based on the results of the study, the hypothesis is validated that there is a poor state of oral health among the population, manifested by the increased incidence of dental infections. International studies confirm that dental infections are often complicated by the presence of comorbidities. Conclusion. The study concludes that patients hospitalized in the department of Oral and Maxillofacial Surgery face a poor oral health state, characterized by a high prevalence of de
背景与目的。本研究在锡比乌县临床急诊医院口腔颌面外科进行，旨在评估该医疗单位转诊患者的口腔健康状况，确定这些患者中是否存在牙科感染以及感染的频率。该研究还旨在确定这些口腔疾病患者的并发症并描述其特征。最后，该研究旨在强调对患者口腔健康状况的关注程度，并为牙科保健服务和制定适当的预防和干预策略提供相关信息。研究方法本研究使用医院的电子病历，以回顾性和描述性的方式进行。样本总数为 1246 人，纳入标准为(a) 妇产科外科住院病人；(b) 需要住院的门诊病人；(c) 按连续住院制度住院的病人；(d) 16 岁以上的病人；(e) 诊断为感染性疾病的病人。数据登记后，考虑了性别、背景、诊断分布、患者的相关病症以及纳入研究的患者是否合并病症等信息。此外，还分析了患者的住院时间、抗生素治疗的需求和持续时间、抗生素组合的使用频率以及与住院时间相关的抗生素使用数量。数据以频率和百分比表示。使用基于关联规则的算法分析了最常见相关病症的组合，并使用和弦图直观地表示这些病症。统计分析使用 IBM SPSS®（社会科学统计软件包）20 版和 R 软件进行。结果样本总数为 1246 人。初步分析显示，样本中大多数人（68.5%）未患传染病，31.5%的人患有传染病。大多数患者来自城市（61.73%），男性（54.34%）。常见合并症包括心脏病（11.99%）和糖尿病（4.08%）。这些数据表明，求助于 OMF 外科服务的患者口腔健康状况很差。这并非个案，而是反映了全球观察到的一种模式。根据研究结果，假设得到了验证，即人口的口腔健康状况较差，表现为牙齿感染的发病率增加。国际研究证实，牙科感染往往因合并症的存在而变得复杂。结论研究得出结论，口腔颌面外科住院病人的口腔健康状况较差，其特点是牙科感染发病率较高。合并症的存在和季节性因素导致了这些感染的加重。这些结果表明，有必要采取预防和教育干预措施，以改善该地区人口的口腔健康状况，并制定个性化的治疗策略。
{"title":"Oral infections - a retrospective study of patients treated in the Oral and Maxillofacial Surgery clinic of the Emergency County Clinical Hospital in Sibiu","authors":"Cosmin Ionuț Lixandru, Ionela Maniu, Mihaela Cernuşcă-Miţariu, C. Domnariu","doi":"10.15386/mpr-2759","DOIUrl":"https://doi.org/10.15386/mpr-2759","url":null,"abstract":"Background and aims. The present study was carried out in the Department of Oral and Maxillo-Facial Surgery of the Sibiu County Emergency Clinical Hospital and aimed to evaluate the oral health status of patients who refer to this medical unit, identifying the presence and frequency of dental infections among these patients. The study also aimed to identify and characterize the comorbidities present in patients with these oral conditions. Finally, the study aims to highlight the degree of concern regarding the oral health status of patients and provide relevant information for dental health services and for the development of appropriate prevention and intervention strategies. \u0000Methods. The study was conducted retrospectively and descriptively, using the hospital’s electronic medical records. The total sample consisted of 1246 people, the inclusion criteria were: (a) patients admitted to the OMF Surgery Department; (b) outpatients who required hospitalization; (c) patients hospitalized under continuous hospitalization regime; (d) patients over 16 years of age; (e) patients diagnosed with infectious pathologies. \u0000After data registration, information on sex, background, distribution of diagnoses, associated pathologies of the patients and the existence of combined pathologies of the patients included in the study were taken into account. The duration of hospitalization of patients, the need and duration of antibiotic therapy, the frequency of use of combinations of antibiotics, and the number of antibiotics used in correlation with the duration of hospitalization were also analyzed. \u0000The data are presented as frequency and percentages. The combination of the most frequent associated pathologies was analyzed using an algorithm based on association rules and chord diagram was used for their visual representation. Statistical analyses were performed using IBM SPSS® (Statistical Package for the Social Science) version 20 and R software. \u0000Results. The total sample consisted of a total of 1246 people. From the preliminary analysis, the majority of people in the sample, 68.5%, did not suffer from infectious conditions, while 31.5% were affected. Most of the patients came from the urban environment (61.73%) and were male (54.34%). Frequent comorbidities included cardiac pathologies (11.99%) and diabetes (4.08%). \u0000These data indicate the poor state of oral health among patients who call on the OMF Surgery services. It is not an isolated case, but it reflects a pattern observed globally. Based on the results of the study, the hypothesis is validated that there is a poor state of oral health among the population, manifested by the increased incidence of dental infections. International studies confirm that dental infections are often complicated by the presence of comorbidities. \u0000Conclusion. The study concludes that patients hospitalized in the department of Oral and Maxillofacial Surgery face a poor oral health state, characterized by a high prevalence of de","PeriodicalId":18438,"journal":{"name":"Medicine and Pharmacy Reports","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141826609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ionut Juravle, E. I. Hirișcău, Tudor Coroi, B. Feciche, Nicolae Crisan, Ioan Coman, Cristian-Doru Pop
Background and aims. To evaluate the quality of life in patients treated for prostate cancer in detail, an accessible, extensive, and easy-to-administer questionnaire is needed. The self-administered 50-item “Expanded Prostate Cancer Index Composite” (EPIC) is an instrument used for this purpose, but it is not officially translated into Romanian. The aim of the study was to translate and validate the Romanian version of the EPIC. Methods. We translated and culturally adapted the EPIC into Romanian. For validation, we included a retrospective analysis of 112 patients who underwent robotic radical prostatectomy and a prospective study including 120 consecutive patients hospitalized before surgical treatment. Baseline and follow-up assessments took place before and at six months, two, and five years post-surgery, between January 2014 and December 2015. We performed cross-sectional correlations between the EPIC, AUASI, and SF-12 at baseline, factor analysis, and calculated the internal consistency. Results. For most EPIC domains and subscales, our a priori-defined criteria for reliability were fulfilled (Cronbach’s alpha 0.7–0.9). Cross-sectional correlations between EPIC scales and AUASI domain ranged from 0.23-0.69, and SF-12 domains ranged from 0.21–0.53 and 0.22–0.61, respectively. The retrospective analysis showed a medium acceptability and understanding of the EPIC questionnaire. In the prospective study, the revised EPIC draft showed an overall higher acceptability with a responding rate of 66% at a 5-year follow-up. All domains exhibited good internal consistency except for the hormonal section (Cronbach’s α = 0.67) at the 6-month follow-up. Conclusion. The Romanian version of the EPIC is reliable, responsive and valid for measuring HRQL in prostate cancer patients. The EPIC questionnaire proved to be an exhaustive and reproducible instrument for evaluating the quality of life in Romanian prostate cancer patients.
{"title":"The Romanian translation and validation of the Extended Prostate Cancer Index Composite in patients undergoing radical prostatectomy","authors":"Ionut Juravle, E. I. Hirișcău, Tudor Coroi, B. Feciche, Nicolae Crisan, Ioan Coman, Cristian-Doru Pop","doi":"10.15386/mpr-2757","DOIUrl":"https://doi.org/10.15386/mpr-2757","url":null,"abstract":"Background and aims. To evaluate the quality of life in patients treated for prostate cancer in detail, an accessible, extensive, and easy-to-administer questionnaire is needed. The self-administered 50-item “Expanded Prostate Cancer Index Composite” (EPIC) is an instrument used for this purpose, but it is not officially translated into Romanian. The aim of the study was to translate and validate the Romanian version of the EPIC. \u0000Methods. We translated and culturally adapted the EPIC into Romanian. For validation, we included a retrospective analysis of 112 patients who underwent robotic radical prostatectomy and a prospective study including 120 consecutive patients hospitalized before surgical treatment. Baseline and follow-up assessments took place before and at six months, two, and five years post-surgery, between January 2014 and December 2015. We performed cross-sectional correlations between the EPIC, AUASI, and SF-12 at baseline, factor analysis, and calculated the internal consistency. \u0000Results. For most EPIC domains and subscales, our a priori-defined criteria for reliability were fulfilled (Cronbach’s alpha 0.7–0.9). Cross-sectional correlations between EPIC scales and AUASI domain ranged from 0.23-0.69, and SF-12 domains ranged from 0.21–0.53 and 0.22–0.61, respectively. The retrospective analysis showed a medium acceptability and understanding of the EPIC questionnaire. In the prospective study, the revised EPIC draft showed an overall higher acceptability with a responding rate of 66% at a 5-year follow-up. All domains exhibited good internal consistency except for the hormonal section (Cronbach’s α = 0.67) at the 6-month follow-up. \u0000Conclusion. The Romanian version of the EPIC is reliable, responsive and valid for measuring HRQL in prostate cancer patients. The EPIC questionnaire proved to be an exhaustive and reproducible instrument for evaluating the quality of life in Romanian prostate cancer patients.","PeriodicalId":18438,"journal":{"name":"Medicine and Pharmacy Reports","volume":" 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141826621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ioana Iurca, Ecaterina Isakescu, L. Pop, Liviuţa Budişan, R. Pîrlog, A. Haranguș, T. Ciuleanu, C. Braicu, I. Berindan-Neagoe
Background and aim. Lung cancer remains one of the most threatening malignancies, ranking as the second most diagnosed cancer, and it continues to be the leading cause of cancer-related deaths worldwide. Challenges persist with late diagnosis and the high mutational burden characteristic of lung cancer. Methods. Our study focuses on identifying the mutational spectrum of a cohort of advanced-stage non-small cell lung cancer (NSCLC) patients using a minimally invasive method through blood collection. To analyze the mutational landscape of these patients, we employed plasma DNA for the next-generation sequencing (NGS) cancer panel Ion Torrent, which contains 50 of the most mutated genes in lung cancer. All protocols for extraction, quality and quantity control, and library preparation follow the manufacturer’s rules. Bioinformatics analysis was performed to select pathogenic mutations versus non-pathogenic-benign ones. Results. This approach is particularly valuable for patients in advanced stages (III and IV, n=10) of lung adenocarcinoma and lung squamous cell carcinoma, who lack surgical options and limited therapeutic avenues. The comprehensive sequencing analysis revealed that nine of the ten lung cancer patients carried a TP53 mutation. Also, several other mutations exist in various cases, showing heterogeneous profiling. Conclusions. Our findings demonstrate the potential of liquid biopsies in providing crucial genetic insights that can guide personalized treatment strategies, improving the management and outcomes for patients with advanced lung cancer.
背景和目的。肺癌仍然是最具威胁性的恶性肿瘤之一，在确诊的癌症中排名第二，并且仍然是全球癌症相关死亡的主要原因。晚期诊断和肺癌特有的高突变负荷一直是肺癌面临的挑战。研究方法我们的研究重点是通过采血这种微创方法确定一批晚期非小细胞肺癌（NSCLC）患者的突变谱。为了分析这些患者的基因突变情况，我们采用血浆 DNA 进行下一代测序（NGS）癌症面板 Ion Torrent 测序，该面板包含 50 个肺癌中突变最多的基因。提取、质量和数量控制以及文库制备的所有方案均遵循制造商的规定。通过生物信息学分析，筛选出致病突变与非致病良性突变。结果。对于缺乏手术选择和治疗途径有限的肺腺癌和肺鳞癌晚期（III 和 IV 期，n=10）患者来说，这种方法尤其有价值。综合测序分析显示，10 名肺癌患者中有 9 人携带 TP53 突变。此外，不同病例中还存在其他几种突变，呈现出异质性特征。结论我们的研究结果表明，液体活检具有提供重要基因信息的潜力，可指导个性化治疗策略，改善晚期肺癌患者的管理和预后。
{"title":"Next-generation sequencing as a valuable tool for mutational spectrum in advanced-stage NSCLC patients","authors":"Ioana Iurca, Ecaterina Isakescu, L. Pop, Liviuţa Budişan, R. Pîrlog, A. Haranguș, T. Ciuleanu, C. Braicu, I. Berindan-Neagoe","doi":"10.15386/mpr-2763","DOIUrl":"https://doi.org/10.15386/mpr-2763","url":null,"abstract":"Background and aim. Lung cancer remains one of the most threatening malignancies, ranking as the second most diagnosed cancer, and it continues to be the leading cause of cancer-related deaths worldwide. Challenges persist with late diagnosis and the high mutational burden characteristic of lung cancer. \u0000Methods. Our study focuses on identifying the mutational spectrum of a cohort of advanced-stage non-small cell lung cancer (NSCLC) patients using a minimally invasive method through blood collection. To analyze the mutational landscape of these patients, we employed plasma DNA for the next-generation sequencing (NGS) cancer panel Ion Torrent, which contains 50 of the most mutated genes in lung cancer. All protocols for extraction, quality and quantity control, and library preparation follow the manufacturer’s rules. Bioinformatics analysis was performed to select pathogenic mutations versus non-pathogenic-benign ones. \u0000Results. This approach is particularly valuable for patients in advanced stages (III and IV, n=10) of lung adenocarcinoma and lung squamous cell carcinoma, who lack surgical options and limited therapeutic avenues. The comprehensive sequencing analysis revealed that nine of the ten lung cancer patients carried a TP53 mutation. Also, several other mutations exist in various cases, showing heterogeneous profiling. \u0000Conclusions. Our findings demonstrate the potential of liquid biopsies in providing crucial genetic insights that can guide personalized treatment strategies, improving the management and outcomes for patients with advanced lung cancer.","PeriodicalId":18438,"journal":{"name":"Medicine and Pharmacy Reports","volume":"8 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141660329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Paun, S. Bolboacă, Radu Chifor, Constantin Radu, Ștefan Strilciuc, I. Badea, C. Borzan
Background and aims. “Severe acute respiratory syndrome coronavirus 2” (SARS-CoV-2) is the name of the etiological agent of the pandemic Corona Virus Disease (COVID-19) which was declared on March 11, 2020 by the WHO and which affected all countries of the world, including Romania. Our study aimed to evaluate the psycho-affective implications and economic consequences for dentists in Romania during the COVID-19 (Corona Virus Disease) pandemic state of emergency and their opinion on the patients’ oral health impact. Methods. A cross-sectional observational and analytical study based on a questionnaire was conducted. Dentists who work in Romania affiliated in 2020 to the Romanian College of Dental Practitioners was the target population. Results. Three hundred and seventy-three dentists participated in this survey. The age of the respondents ranged from 24 to 70. Men and women have the same expectation regarding the lockdown effect of worsening the oral health of the general population (worst, 79.5% of women and 81.3% of men, P=0.8842). Conclusions. The anxiety regarding the bank rates, supplementary investments, risk of COVID-19 infection and exposure to COVID-19 related mass-media information proved to be significantly higher among young dentists.
{"title":"Dentists’ stress level during the COVID-19 pandemic and their opinion on the oral health status of their patients","authors":"A. Paun, S. Bolboacă, Radu Chifor, Constantin Radu, Ștefan Strilciuc, I. Badea, C. Borzan","doi":"10.15386/mpr-2764","DOIUrl":"https://doi.org/10.15386/mpr-2764","url":null,"abstract":"Background and aims. “Severe acute respiratory syndrome coronavirus 2” (SARS-CoV-2) is the name of the etiological agent of the pandemic Corona Virus Disease (COVID-19) which was declared on March 11, 2020 by the WHO and which affected all countries of the world, including Romania. Our study aimed to evaluate the psycho-affective implications and economic consequences for dentists in Romania during the COVID-19 (Corona Virus Disease) pandemic state of emergency and their opinion on the patients’ oral health impact. \u0000Methods. A cross-sectional observational and analytical study based on a questionnaire was conducted. Dentists who work in Romania affiliated in 2020 to the Romanian College of Dental Practitioners was the target population. \u0000Results. Three hundred and seventy-three dentists participated in this survey. The age of the respondents ranged from 24 to 70. Men and women have the same expectation regarding the lockdown effect of worsening the oral health of the general population (worst, 79.5% of women and 81.3% of men, P=0.8842). \u0000Conclusions. The anxiety regarding the bank rates, supplementary investments, risk of COVID-19 infection and exposure to COVID-19 related mass-media information proved to be significantly higher among young dentists.","PeriodicalId":18438,"journal":{"name":"Medicine and Pharmacy Reports","volume":"3 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141662747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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