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Microscope image reconstruction 显微镜图像重建
Pub Date : 1900-01-01 DOI: 10.1364/srs.1998.stue.2
C. Sheppard
In brightfield, phase-contrast or polarization microscopy, the image can be modeled by using scattering theory. The object, consisting of spatial variations in complex refractive index, scatters components of an angular spectrum of plane waves, and the image calculated by integration over incident and scattered waves. This approach takes into account the high aperture effects, important in microscope imaging. Rigorous methods can be used to calculate the scattering by the object.1 However, these methods, in addition to being in general very computationally intensive, result in the disadvantges that it is difficult to see trends in the behaviour and usually impracticable to reconstruct the object from the image data.
在明场显微镜、相衬显微镜或偏振显微镜中,可以使用散射理论对图像进行建模。该物体由复折射率的空间变化组成,散射平面波的角谱分量,并通过对入射波和散射波的积分计算出图像。这种方法考虑到了显微镜成像中重要的大孔径效应。可以采用严格的方法来计算物体的散射然而,这些方法除了通常计算量非常大之外,还存在难以看到行为趋势和通常无法从图像数据中重建物体的缺点。
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引用次数: 0
Multiframe iterative blind deconvolution using only a positivity constraint 仅使用正约束的多帧迭代盲反卷积
Pub Date : 1900-01-01 DOI: 10.1364/srs.1998.stua.3
D. Biggs, M. Andrews
The problem of blind deconvolution, extracting both the original image and point spread function (PSF) from only the measurement of their convolution, may at first seem a futile task. However Lane and Bates [1] have shown via separation of zero sheets that blind deconvolution is theoretically possible for dimensions of two or greater when very little noise is present. In practice noise is a significant problem that limits the ability to perform successful deconvolution. Many researchers have also found that the only way of achieving blind deconvolution is to impose spatial and spectral constraints on the solution, derived from a priori information about the imaging system, and to eliminate the trivial solution of the image or PSF being a delta function. This becomes less useful in situations where very little else is known except the measured data.
盲反卷积问题,即仅通过测量原始图像和点扩散函数(PSF)的卷积来提取原始图像,乍一看似乎是一项徒劳的任务。然而,Lane和Bates[1]已经通过零片的分离表明,当存在很少的噪声时,对于两个或更大的维度,盲反卷积在理论上是可能的。在实际应用中,噪声是限制成功反褶积能力的一个重要问题。许多研究人员还发现,实现盲反褶积的唯一方法是对从成像系统的先验信息中导出的解施加空间和光谱约束,并消除图像或PSF作为delta函数的平凡解。在除了测量数据之外几乎一无所知的情况下,这就不那么有用了。
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引用次数: 2
High Resolution Imaging of Astronomical Objects Using Deconvolution from Wave-Front Sensing Experimental Demonstration 基于波前传感反褶积的天文目标高分辨率成像实验演示
Pub Date : 1900-01-01 DOI: 10.1364/srs.1995.rwa4
D. Dayton, S. Sandven, J. Gonglewski
It is well known that the image forming ability of an astronomical optic system is limited by distortions due to a turbulent atmosphere1. A large aperture telescope will have no more resolving power than one with a diameter equal to Fried’s rO parameter which is usually between 5 and 10 centimeters. Recently, technological development in the areas of wave-front sensing and deformable mirrors have lead to adaptive optic systems. These systems attempt to measure the atmosphere induced wave-front distortion and cancel it through the use of a deformable mirror. Due to the short correlation time of the atmosphere, they require a high bandwidth multi-input multi-output control system.
众所周知,天文光学系统的成像能力受到湍流大气造成的畸变的限制。大口径望远镜的分辨能力不会比直径等于弗里德rO参数(通常在5到10厘米之间)的望远镜高。近年来,在波前传感和变形镜领域的技术发展导致了自适应光学系统的出现。这些系统试图测量大气引起的波前畸变,并通过使用可变形镜来消除它。由于大气的相关时间短,需要高带宽的多输入多输出控制系统。
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引用次数: 0
Retrieval of Physical Properties of Atmospheric Particles by Inversion via Regularization in the Limit of a Small Optical Data Set 小光学数据集条件下正则化反演反演大气粒子物理性质
Pub Date : 1900-01-01 DOI: 10.1364/srs.1998.sthc.4
D. Müller, A. Ansmann, U. Wandinger, D. Althausen
Atmospheric aerosols, although only a minor constituent of the earth’s atmosphere, play an important role in many atmospheric processes. Due to their appreciable influence on the earth’s radiation budget, air quality, clouds and precipitation as well as the chemistry of the troposphere and stratosphere it is necessary to gather detailed information on their optical and physical properties. A multiple-wavelength lidar as well as a Raman lidar at the Institute provide optical particle information in terms of six backscatter coefficients and two extinction coefficients in the wavelength range from 0.355 to 1.064 μm on a vertical scale. A data-evaluation algorithm that uses the method of inversion via regularization has been specifically designed to retrieve physical properties from the given optical information. The physical parameters can be described by, e.g., the particle size distributions, the mean sizes derived from it, like the effective radius, the volume, surface-area, and number concentrations as well as the complex refractive index. Due to the low amount of available a priori information on the particle properties in combination with the small number of optical information that additionally include large measurement errors the main focus had been on the retrieval of the mean values. To control the quality of the regularization under these difficult conditions the method of generalized cross-validation is used as it does not require the knowledge of the underlying measurement errors nor the knowledge of the specific shape of the particle size distributions.
大气气溶胶虽然只占地球大气的一小部分,但在许多大气过程中起着重要作用。由于它们对地球的辐射收支、空气质量、云和降水以及对流层和平流层的化学有明显的影响,因此有必要收集关于它们的光学和物理性质的详细资料。该研究所的多波长激光雷达和拉曼激光雷达在0.355 ~ 1.064 μm的垂直波长范围内提供了6个后向散射系数和2个消光系数的光学粒子信息。为了从给定的光学信息中检索物理性质,专门设计了一种使用正则化反演方法的数据评估算法。物理参数可以用粒径分布、平均粒径、有效半径、体积、表面积、粒子数浓度以及复折射率等来描述。由于关于粒子性质的先验信息很少,加上光学信息数量少,另外还包括较大的测量误差,因此主要的重点是均值的检索。为了在这些困难的条件下控制正则化的质量,使用了广义交叉验证的方法,因为它不需要了解潜在的测量误差,也不需要了解粒度分布的特定形状。
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引用次数: 0
Bispectral Imaging on Adaptive Optics Compensated Data 自适应光学补偿数据的双光谱成像
Pub Date : 1900-01-01 DOI: 10.1364/srs.1995.rwa3
P. Negrete-Regagnon, J. Drummond, R. Fugate, J. Gonglewski, N. Wooder, J. Dainty
The release of military technology in the adaptive optics (AO) and laser beacons fields [1] motivated the astronomical community to become actively involved in its implementation in astronomical sites all around the world. Unfortunately, even with the impressive gain in resolution these systems provide, high-resolution imaging from ground-based telescopes is still far from ideal. Traditional speckle interferometry techniques and post-processing data reduction are probably the only viable way to obtain approximated diffraction-limited images. This work describes the application of a posteriori processing to both AO compensated and uncompensated data and shows the improvement that can be achieved.
军事技术在自适应光学(AO)和激光信标领域的发布[1]促使天文学界积极参与其在世界各地天文站点的实施。不幸的是,即使这些系统提供了令人印象深刻的分辨率增益,地面望远镜的高分辨率成像仍然远远不够理想。传统的散斑干涉技术和后处理数据简化可能是获得近似衍射限制图像的唯一可行方法。这项工作描述了对AO补偿和未补偿数据的后测处理的应用,并展示了可以实现的改进。
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引用次数: 0
Inverse problems in reflection seismology 反射地震学中的反演问题
Pub Date : 1900-01-01 DOI: 10.1364/srs.1998.stha.1
J. Claerbout
The international oil industry spends about $4 billion/year acquiring reflection seismic data. A large survey collects a terabyte (1012) of data which is computer processed to a pixel volume of (103)3 = 109 bytes. At sea the energy source is usually an air gun while on land, the source is mostly buried dynamite, and sometimes, multiple trucks carrying sweep- frequency ground vibrators. At sea, a shot is fired every 10 seconds; echos are recorded along 6 km cables at about a thousand locations, each channel recording a signal of about 2000 floating point values. A typical marine survey contract whose result is shown in Figure 1, lasts a month or more and costs upward of $10M.
国际石油行业每年花费约40亿美元来获取反射地震数据。一项大型调查收集了1tb(1012)的数据,这些数据被计算机处理成(103)3 = 109字节的像素体积。在海上,能量源通常是气枪,而在陆地上,能量源大多是埋在地下的炸药,有时,多辆卡车携带扫频地面振动器。在海上,每10秒发射一枪;回声沿着6公里长的电缆在大约1000个地点被记录下来,每个通道记录一个大约2000个浮点值的信号。一份典型的海上测量合同,其结果如图1所示,持续一个月或更长时间,成本超过1000万美元。
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引用次数: 0
Support-Constrained Motion-Artifact Correction for Magnetic Resonance Imaging 支持约束运动伪影校正的磁共振成像
Pub Date : 1900-01-01 DOI: 10.1364/srs.1995.rtua4
J. Fienup, J. E. Van Buhler
Motion during the collection of a magnetic resonance imaging (MRI) data set causes phase errors which result in a smearing or ghosting of the image. In this paper we present a new algorithm for correcting translational motion errors. It follows the same philosophy as the gradient search approaches that we invented to determine the aberrations of the Hubble Space Telescope [1] and to correct phase errors for synthetic-aperture radar [2].
在磁共振成像(MRI)数据集的收集过程中,运动会导致相位误差,从而导致图像的涂抹或重影。本文提出了一种修正平移运动误差的新算法。它遵循与梯度搜索方法相同的原理,我们发明了梯度搜索方法来确定哈勃太空望远镜的像差[1]和纠正合成孔径雷达的相位误差[2]。
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引用次数: 0
Coherence function as carrier of optical information 相干函数作为光信息的载体
Pub Date : 1900-01-01 DOI: 10.1364/srs.1998.swb.4
A. Lohmann, D. Mendlovic, G. Shabtay
A major characteristic of an optical wave is its coherence function function which is defined by: where V is the complex amplitude and the brackets stand for ensemble averaging.
光波的一个主要特征是它的相干函数,其定义为:其中V是复振幅,括号表示系综平均。
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引用次数: 0
Recovering Fourier phase data from amplitude data alone in macromolecular X-ray crystallography 大分子x射线晶体学中仅从振幅数据恢复傅立叶相位数据
Pub Date : 1900-01-01 DOI: 10.1364/srs.1995.rtub1
S. Subbiah
The ab initio phase problem is the rate-limiting step in X-ray crystallography and by extension is also a rate-limiting step in the determination of high-resolution 3-dimensional (3-D) structures of biological macromolecules. Thus it has dramatic repercussions for the emerging areas of rational drug-design and protein engineering. Simply stated, the problem is of recovering the real-space image of a biological macromolecule - represented in Cartesian 3-D coordinates-from partial Fourier information alone. Specifically, the available information in reciprocal space is that of the Fourier amplitudes; the complementary Fourier phase information is not available.
从头算相问题是x射线晶体学的限速步骤,推而广之,也是确定生物大分子高分辨率三维结构的限速步骤。因此,它对理性药物设计和蛋白质工程等新兴领域产生了巨大的影响。简单地说,问题是仅从部分傅里叶信息恢复生物大分子的实空间图像-以笛卡尔三维坐标表示。具体来说,互反空间中可用的信息是傅里叶振幅的信息;互补的傅里叶相位信息不可用。
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引用次数: 0
Material characterization via phaseless tomography : numerical results in phase retrieval 通过无相层析成像表征材料:相位检索的数值结果
Pub Date : 1900-01-01 DOI: 10.1364/srs.1998.sthc.3
R. Pierri, G. Leone, R. Bernini
The diffraction tomography is a new method in which the complex permittivity profile of an object is reconstructed using algorithms that require the knowledge of both the amplitude and the phase of the scattered field [1-2]. However the difficulty of obtaining the phase of the scattered field at optical frequencies has limited the practical applications of diffraction tomography.
衍射层析成像是一种新的方法,它使用需要同时知道散射场的振幅和相位的算法来重建物体的复介电常数曲线[1-2]。然而,衍射层析成像在光学频率下难以获得散射场的相位,这限制了衍射层析成像的实际应用。
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引用次数: 1
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Signal Recovery and Synthesis
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