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Canterbury Workshop and School in Optical Coherence Tomography and Adaptive Optics最新文献

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Challenges and possibilities for developing adaptive optics: ultra-high resolution optical coherence tomography for clinical in vivo retinal imaging 发展自适应光学的挑战和可能性:用于临床体内视网膜成像的超高分辨率光学相干断层扫描
R. Zawadzki, Stacey S. Choi, J. W. Evans, J. Werner
Recent developments in adaptive optics - optical coherence tomography (AO-OCT) allow for ultra-high isotropic resolution imaging of the in-vivo retina, offering unprecedented insight into its volumetric microscopic and cellular structures. In addition to this promising achievement, the clinical impact and application of this technology still needs to be explored. This includes assessment of limitations and challenges for existing as well as future AO-OCT systems, especially in the context of potential transfer of this technology from an optical bench to a portable imaging system. To address these issues we will describe our current AO-UHR-OCT focusing on its sub-components, as well as application for clinical imaging. Additionally, we describe some directions for future development of our AO-OCT instrument that would improve its clinical utility including: new compact AO-OCT design, new improved AO sub-system (extreme AO), and new generations of Fourier-domain-OCT.
自适应光学的最新发展-光学相干断层扫描(AO-OCT)允许对体内视网膜进行超高各向同性分辨率成像,为其体积微观和细胞结构提供前所未有的见解。除了这一有希望的成果外,该技术的临床影响和应用仍有待探索。这包括对现有和未来AO-OCT系统的局限性和挑战的评估,特别是在该技术从光学平台转移到便携式成像系统的潜在背景下。为了解决这些问题,我们将介绍我们目前的AO-UHR-OCT,重点是其子组件,以及临床成像的应用。此外,我们描述了我们的AO- oct仪器的未来发展方向,以提高其临床应用,包括:新的紧凑型AO- oct设计,新的改进AO子系统(极端AO),以及新一代的傅里叶域oct。
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引用次数: 0
Multiple delay lines full-field optical coherence tomography 多延迟线全场光学相干层析成像
Jingyu Wang, C. Dainty, A. Podoleanu
Full-field Optical Coherence Tomography (FF-OCT) is a parallel detection OCT technique using a 2D detector array. This technique avoids mechanical scanning in imaging optics. Therefore, it can speed up the imaging process and enhance the imaging quality. We present a FF-OCT instrument to be used in conjunction with the principle of multiple delays (MD) OCT to evaluate the topography of curved objects in a single-shot imaging. We evaluate the optimum combination of the MD principle with the FF-OCT method and measure the radius of a metal ball with this method. We managed to obtain 2n-1 contour lines using an MDE with n delays in a single en-face OCT image to evaluate the curvature of the object surface.
全文场光学相干层析成像(FF-OCT)是一种利用二维探测器阵列进行平行探测的OCT技术。该技术避免了成像光学中的机械扫描。因此,它可以加快成像过程,提高成像质量。我们提出了一种FF-OCT仪器,用于结合多重延迟(MD) OCT原理,在单次成像中评估弯曲物体的地形。我们评估了MD原理与FF-OCT方法的最佳组合,并用该方法测量了金属球的半径。我们设法在单个正面OCT图像中使用具有n个延迟的MDE获得2n-1等高线,以评估物体表面的曲率。
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引用次数: 5
Doppler optical coherence tomography in cardiovascular physiology 多普勒光学相干断层扫描在心血管生理学中的应用
M. Bonesi, I. Meglinski, S. Matcher
The study of flow dynamics in complex geometry vessels is highly important in many biomedical applications where the knowledge of the mechanic interactions between the moving fluid and the housing media plays a key role for the determination of the parameters of interest, including the effect of blood flow on the possible rupture of atherosclerotic plaques. Doppler Optical Coherence Tomography (DOCT), as a functional extension of Optical Coherence Tomography (OCT), is an optic, non-contact, non-invasive technique able to achieve detailed analysis of the flow/vessel interactions. It allows simultaneous high resolution imaging (10 μm typical) of the morphology and composition of the vessel and determination of the flow velocity distribution along the measured cross-section. We applied DOCT system to image high-resolution one-dimensional and multi-dimensional velocity distribution profiles of Newtonian and non-Newtonian fluids flowing in vessels with complex geometry, including Y-shaped and T-shaped vessels, vessels with aneurism, bifurcated vessels with deployed stent and scaffolds. The phantoms were built to mimic typical shapes of human blood vessels, enabling preliminary analysis of the interaction between flow dynamics and the (complex) geometry of the vessels and also to map the related velocity profiles at several inlet volume flow rates. Feasibility studies for quantitative observation of the turbulence of flows arising within the complex geometry vessels are discussed. In addition, DOCT technique was also applied for monitoring cerebral mouse blood flow in vivo. Two-dimensional DOCT images of complex flow velocity profiles in blood vessel phantoms and in vivo sub-cranial mouse blood flow velocities distributions are presented.
复杂几何血管的流动动力学研究在许多生物医学应用中非常重要,其中运动流体和外壳介质之间的力学相互作用的知识对于确定感兴趣的参数起着关键作用,包括血液流动对动脉粥样硬化斑块可能破裂的影响。多普勒光学相干断层扫描(DOCT)作为光学相干断层扫描(OCT)的功能延伸,是一种光学、非接触、非侵入性技术,能够实现对血流/血管相互作用的详细分析。它可以同时对容器的形态和组成进行高分辨率成像(典型10 μm),并确定沿测量截面的流速分布。我们应用DOCT系统对复杂几何形状的血管(包括y形血管和t形血管、动脉瘤血管、支分叉血管和支架)中牛顿流体和非牛顿流体的一维和多维速度分布进行了高分辨率成像。这些模型是为了模仿人类血管的典型形状而建造的,可以初步分析血流动力学和血管(复杂的)几何形状之间的相互作用,并绘制出不同入口体积流速下的相关速度分布图。讨论了对复杂几何容器内流动湍流进行定量观测的可行性研究。此外,还应用DOCT技术监测脑鼠体内血流量。本文给出了血管幻象中复杂血流速度分布的二维DOCT图像和活体小鼠颅下血流速度分布。
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引用次数: 0
High-speed high-resolution optical coherence tomography at 800 and 1060 nm 800和1060纳米的高速高分辨率光学相干层析成像
B. Povazay, B. Hofer, B. Hermann, C. Torti, Vedran Kajic, A. Unterhuber, W. Drexler
Two high speed systems for spectrometer based frequency domain optical coherence tomography are presented. A device operating at 800 nm, based on the Basler Sprint CMOS camera with linerates of up to 312,000 lps and a device based on the Goodrich SUI LHD 1024 px camera at 1060 nm with 47,000 lps are applied in a clinical environment to normal subjects. The feasibility of clinical high and ultra high-resolution optical coherence tomography (OCT) devices for retinal imaging at different wavelengths, capable of isotropic sampling with 70 to 600 frames per second at 512 depth scans/frame for widefield imaging and high density sampling at 1 Gvoxel are demostrated.
介绍了两种基于谱仪的高速频域光学相干层析成像系统。一个基于Basler Sprint CMOS相机的800 nm工作设备,其线性高达312,000 lp,一个基于Goodrich SUI LHD 1024像素相机的1060 nm设备,其线性高达47,000 lp,应用于临床环境中,用于正常受试者。研究了应用于不同波长视网膜成像的临床高分辨率和超高分辨率光学相干断层扫描(OCT)设备的可行性,这些设备能够以每秒70至600帧的速度进行各向同性采样,以512帧深度扫描/帧进行宽视场成像和1 Gvoxel的高密度采样。
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引用次数: 4
Theory and applications of multi-beam OCT 多束OCT的理论与应用
J. Holmes
The lateral resolution of Fourier domain optical coherence tomography (FD-OCT) systems is limited by the depth of focus that can be achieved over the desired imaging depth at the chosen wavelength. I present a solution in which multiple beams focused at different depths are scanned together, so that a mosaic image can be produced, with at least double the resolution possible from a single beam system. Side-benefits also arise from the ability to combine data from each beam to improve signal-to-noise. The theory of this approach is discussed, advantages, and also the practical realisation in a variety of ex-vivo and in-vivo OCT imaging probes, together with results from a number of applications.
傅里叶域光学相干层析成像(FD-OCT)系统的横向分辨率受到所选波长下可达到的期望成像深度的聚焦深度的限制。我提出了一种解决方案,其中聚焦在不同深度的多个光束一起扫描,这样就可以产生马赛克图像,其分辨率至少是单光束系统的两倍。附带的好处还包括能够将每个波束的数据组合起来,以改善信噪比。讨论了该方法的理论,优点,以及在各种离体和体内OCT成像探针中的实际实现,以及许多应用的结果。
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引用次数: 22
Nanoparticles for enhanced contrast optical coherence tomography 用于增强对比度光学相干断层扫描的纳米颗粒
C. Maule, P. Quaresma, P. Carvalho, P. Jorge, E. Pereira, C. Rosa
Recently the area of bioimaging has benefited from new types of image enhancing agents such as quantum dots, carbon nanotubes and other nanoparticles. Cellular or even molecular level resolution has been achieved with different techniques during these last years (i.a. Fluorescence microscopy, PET/CT scan, AFM). Optical Coherence Tomography (OCT) as an imaging technique should also profit from newly developed probes. In this work we explored the tunable properties of different types of nanoparticles as contrast enhancers in OCT applications. We mainly studied the development and characteristics of metallic nanoparticles with tunable properties: gold nanoshells made of a silica core coated with a gold shell. Nanoshell and nanoparticles processing techniques are discussed, as well as their optimization for designing particles with specific absorption and scattering characteristics, and its use in OCT imaging.
最近,生物成像领域受益于新型图像增强剂,如量子点、碳纳米管和其他纳米颗粒。在过去的几年里,细胞甚至分子水平的分辨率已经通过不同的技术(如荧光显微镜,PET/CT扫描,AFM)实现了。光学相干层析成像(OCT)作为一种成像技术也将受益于新开发的探针。在这项工作中,我们探索了不同类型的纳米颗粒作为OCT应用中的对比度增强剂的可调特性。我们主要研究了具有可调谐性能的金属纳米粒子的发展和特性:由二氧化硅核包覆金壳制成的金纳米壳。讨论了纳米壳和纳米颗粒的加工技术,以及它们在设计具有特定吸收和散射特性的颗粒方面的优化,以及它们在OCT成像中的应用。
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引用次数: 2
En-face OCT microleakage investigation after laser-assisted dental hard tissue treatment 激光辅助牙硬组织治疗后的正面OCT微渗漏研究
C. Todea, Cosmin Bălăbuc, C. Sinescu, M. Negruțiu, Laura Filip, A. Bradu, A. Podoleanu
Purpose: To investigate using en-face Optical Coherence Tomography (OCT) the microleakage after Er:YAG laser cavity preparation. Material and Methods: Thirty single- and multi-rooted freshly extracted human teeth divided into two study groups, group I (laser) and group II (control), were used in this study. In group I, Class V cavities were prepared using laser on the buccal surface of each tooth. The laser device used was an Er:YAG laser (2940 nm, VSP, 250-320 mJ, 10-20 Hz). In group II, cavities were prepared conventionally and acid etched on the oral surface of the same tooth. All cavities were filled with composite resin. The cavity microleakage was investigated using en-face Optical Coherence Tomography prototype, based on transverse scanning and operating at 1300 nm. Results: The investigation demonstrated qualitatively the reduction of microleakage in cavities prepared with Er:YAG laser as compared to the control group, in which cavities were prepared conventionally. Conclusion: The en-face OCT method provided a superior non-invasive and real time investigation method, thus reducing the occurrence of secondary caries by early detection. Moreover, based on the results of this investigation, it may be concluded that Er:YAG laser-assisted cavity preparation leads to reduction of microleakage.
目的:利用面光学相干断层扫描(OCT)研究Er:YAG激光腔制备后的微泄漏。材料与方法:将30颗新鲜拔除的人单根和多根牙齿分为激光组和对照组。在第一组,用激光在每颗牙齿的颊面制备V类空腔。激光器件为Er:YAG激光器(2940 nm, VSP, 250 ~ 320 mJ, 10 ~ 20 Hz)。在第二组中,常规制备空腔,并在同一颗牙齿的口腔表面进行酸蚀。所有空腔均用复合树脂填充。采用横向扫描的正面光学相干层析成像原型机,在1300 nm波长下对腔体微泄漏进行了研究。结果:与常规制备腔体的对照组相比,研究定性地证明了Er:YAG激光制备腔体的微泄漏减少。结论:正面OCT法是一种无创、实时的检查方法,早期发现可减少继发龋的发生。此外,基于本研究的结果,可以得出Er:YAG激光辅助空腔制备导致微泄漏减少的结论。
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引用次数: 0
Optical Coherence Tomography for non-destructive investigations of structure of easel paintings 用于对架上绘画结构进行非破坏性研究的光学相干断层扫描技术
E. Kwiatkowska, M. Sylwestrzak, Bogumiła J. Rouba, L. Tymińska-Widmer, M. Iwanicka, P. Targowski
In this contribution the application of Optical Coherence Tomography (OCT) for non-invasive structural imaging of easel paintings will be presented. Since the technique permits imaging semi-transparent layers accessible for infrared light, the varnish and glaze layers are usually under investigation. The major emphasis will be laid on application of OCT to resolving specific conservation problems, arising during the restoration process. The examples of imaging multilayer varnishes and subsequent alterations will be given and the application of these images for authentication of inscriptions will be discussed. Since the thickness of imaged layers may be directly measured with OCT in completely non-destructive, quick and convenient way as many times as necessary, the application of the technique to generation of varnish thickness maps will be presented.
本文将介绍光学相干断层扫描(OCT)在架上绘画无创结构成像中的应用。由于该技术可以对红外线照射到的半透明层进行成像,因此通常会对清漆层和釉层进行研究。主要重点是应用 OCT 技术解决修复过程中出现的具体保护问题。将举例说明多层清漆的成像和随后的改动,并讨论这些图像在鉴定碑文方面的应用。由于成像层的厚度可以通过 OCT 以完全无损、快速和方便的方式直接测量,而且可以根据需要多次测量,因此将介绍该技术在生成清漆厚度图方面的应用。
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引用次数: 4
Optical coherence tomography for the investigation of posterior and anterior eye segments 光学相干断层扫描研究后、前眼段
Y. Yasuno
The applications of three-dimensional Fourier domain optical coherence tomography (FD-OCT) to ophthalmology are demonstrated. The fundamentals of two types of FD-OCT, i.e., spectral domain OCT (SD-OCT) and swept source OCT (SS-OCT) are described. The 3D investigation of age-related macular degeneration and keratoconus are presented.
介绍了三维傅里叶域光学相干断层扫描(FD-OCT)在眼科中的应用。介绍了两种FD-OCT的基本原理,即谱域OCT (SD-OCT)和扫频源OCT (SS-OCT)。本文介绍老年性黄斑变性及圆锥角膜的三维影像。
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引用次数: 0
An innovative approach for investigating the ceramic bracket-enamel interface - optical coherence tomography and confocal microscopy 一种研究陶瓷支架-牙釉质界面的创新方法-光学相干层析成像和共聚焦显微镜
R. Romînu, C. Sinescu, M. Rominu, M. Negruțiu, Philippe Laissue, S. Mihali, L. Cuc, M. Hughes, A. Bradu, A. Podoleanu
Bonding has become a routine procedure in several dental specialties - from prosthodontics to conservative dentistry and even orthodontics. In many of these fields it is important to be able to investigate the bonded interfaces to assess their quality. All currently employed investigative methods are invasive, meaning that samples are destroyed in the testing procedure and cannot be used again. We have investigated the interface between human enamel and bonded ceramic brackets non-invasively, introducing a combination of new investigative methods - optical coherence tomography (OCT) and confocal microscopy (CM). Brackets were conventionally bonded on conditioned buccal surfaces of teeth The bonding was assessed using these methods. Three dimensional reconstructions of the detected material defects were developed using manual and semi-automatic segmentation. The results clearly prove that OCT and CM are useful in orthodontic bonding investigations.
粘合已成为许多牙科专业的常规程序-从修复学到保守牙科甚至正畸学。在许多这些领域中,能够研究键合界面以评估其质量是很重要的。目前使用的所有调查方法都是侵入性的,这意味着样品在测试过程中被破坏,不能再次使用。我们研究了人类牙釉质和粘结陶瓷支架之间的非侵入性界面,引入了新的研究方法-光学相干断层扫描(OCT)和共聚焦显微镜(CM)的组合。托槽通常粘接在牙齿的条件颊面上,使用这些方法评估粘接。采用手工和半自动分割对检测到的材料缺陷进行三维重建。结果清楚地证明OCT和CM在正畸粘接调查中是有用的。
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引用次数: 3
期刊
Canterbury Workshop and School in Optical Coherence Tomography and Adaptive Optics
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