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Reduction of measurement errors in OCT scanning 减少OCT扫描测量误差
E. Morel, P. M. Tabla, M. Sallese, J. Torga
Optical coherence tomography (OCT) is a non-destructive optical technique, which uses a light source with a wide band width that focuses on a point in the sample to determine the distance (strictly, the optical path difference, OPD) between this point and a reference surface. The point can be superficial or at an interior interface of the sample (transparent or semitransparent), allowing topographies and / or tomographies in different materials. The Michelson interferometer is the traditional experimental scheme for this technique, in which a beam of light is divided into two arms, one the reference and the other the sample. The overlap of reflected light in the sample and in the reference generates an interference signal that gives us information about the OPD between arms. In this work, we work on the experimental configuration in which the reference signal and the reflected signal in the sample travel on the same arm, improving the quality of the interference signal. Among the most important aspects of this improvement we can mention that the noise and errors produced by the relative reference–sample movement and by the dispersion of the refractive index are considerably reduced. It is thus possible to obtain 3D images of surfaces with a spatial resolution in the order of microns. Results obtained on the topography of metallic surfaces, glass and inks printed on paper are presented.
光学相干层析成像(OCT)是一种非破坏性的光学技术,它使用具有宽频带宽度的光源聚焦在样品中的一个点上,以确定该点与参考表面之间的距离(严格地说,光程差,OPD)。点可以是表面的,也可以在样品的内部界面上(透明或半透明),允许在不同的材料中进行地形和/或层析成像。迈克尔逊干涉仪是这项技术的传统实验方案,其中一束光被分成两臂,一臂是参考物,另一臂是样品。样品和参考中反射光的重叠产生干涉信号,该信号为我们提供了臂间OPD的信息。在这项工作中,我们研究了样品中的参考信号和反射信号在同一臂上行进的实验配置,从而提高了干扰信号的质量。在这种改进的最重要的方面中,我们可以提到由相对参考样品运动和由折射率色散产生的噪声和误差大大减少了。因此,可以获得具有微米级空间分辨率的表面的三维图像。给出了在金属表面、玻璃表面和印刷在纸上的油墨表面形貌上得到的结果。
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引用次数: 4
Impact of absorption in the top layer of a two layer sample on spectroscopic spectral domain interferometry of the bottom layer 两层样品顶层吸收对底层光谱域干涉测量的影响
F. Fleischhauer, T. Feuchter, L. Leick, R. Rajendram, A. Podoleanu
Spectroscopic spectral domain interferometry and spectroscopic optical coherence tomography combine depth information with spectrally-resolved localised absorption data. These additional data can improve diagnostics by giving access to functional information of the investigated sample. One possible application is measuring oxygenation levels at the retina for earlier detection of several eye diseases. Here measurements with different hollow glass tube phantoms are shown to measure the impact of a superficial absorbing layer on the precision of reconstructed attenuation spectra of a deeper layer. Measurements show that a superficial absorber has no impact on the reconstructed absorption spectrum of the deeper absorber. Even when diluting the concentration of the deeper absorber so far that an incorrect absorption maximum is obtained, still no influence of the superficially placed absorber is identified.
光谱光谱域干涉测量和光谱光学相干层析成像结合深度信息与光谱分辨局部吸收数据。这些额外的数据可以通过访问被调查样本的功能信息来改进诊断。一种可能的应用是测量视网膜的氧化水平,以便早期发现几种眼病。本文用不同的中空玻璃管模型测量了表层吸收层对深层吸收层重构衰减光谱精度的影响。测量结果表明,表面吸收剂对深层吸收剂的重构吸收光谱没有影响。即使将较深吸收剂的浓度稀释到一个不正确的吸收最大值,仍然没有发现浅层吸收剂的影响。
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引用次数: 0
Increasing signal-to-noise ratio of swept-source optical coherence tomography by oversampling in k-space k空间过采样提高扫描源光学相干层析成像的信噪比
Karim Nagib, Biniyam Mezgebo, Rahul Thakur, Namal Fernando, B. Kordi, S. Sherif
Optical coherence tomography systems suffer from noise that could reduce ability to interpret reconstructed images correctly. We describe a method to increase the signal-to-noise ratio of swept-source optical coherence tomography (SSOCT) using oversampling in k-space. Due to this oversampling, information redundancy would be introduced in the measured interferogram that could be used to reduce white noise in the reconstructed A-scan. We applied our novel scaled nonuniform discrete Fourier transform to oversampled SS-OCT interferograms to reconstruct images of a salamander egg. The peak-signal-to-noise (PSNR) between the reconstructed images using interferograms sampled at 250MS/s andz50MS/s demonstrate that this oversampling increased the signal-to-noise ratio by 25.22 dB.
光学相干层析成像系统受到噪声的影响,噪声会降低正确解释重建图像的能力。我们描述了一种利用k空间过采样来提高扫描源光学相干层析成像(SSOCT)信噪比的方法。由于这种过采样,被测干涉图中会引入信息冗余,可以用来降低重构a扫描中的白噪声。我们将新颖的缩放非均匀离散傅里叶变换应用于过采样的SS-OCT干涉图,以重建蝾螈卵的图像。250MS/s和z50ms /s采样的干涉图重建图像的峰值信噪比(PSNR)表明,这种过采样使信噪比提高了25.22 dB。
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引用次数: 0
Subretinal hyper-reflective material seen on optical coherence tomography as a biomarker for disease monitoring in age-related macular degeneration 在光学相干断层扫描上看到的视网膜下高反射物质作为年龄相关性黄斑变性疾病监测的生物标志物
H. B. Lee, B. Ong, M. Katta, C. Yvon, L. Lu, R. Zakri, N. Patel
Subretinal hyper-reflective material (SHRM) seen on optical coherence tomography (OCT) is thought to be a collection of fibrous tissues and vascular networks that are identified in age-related macular degeneration (ARMD). We have carried out a retrospective analysis of 91 OCT scans of neovascular ARMD subtypes including classic and occult choroidal neovascularization (CNV) and retinal angiomatous proliferation (RAP). All three subtypes received ranibizumab, an anti-vascular endothelial growth factor (Anti-VEGF) intravitreal injections on an as-needed basis following the loading doses. Volumes of SHRM were calculated using caliper measurements of maximal height and length of SHRM seen on OCT. The ellipsoid formula derived from tumour models was used to calculate the volume. It was found that occult CNV and RAP have larger SHRM volumes than those of classic CNV. SHRM volumes reduced overall following loading doses of Anti-VEGF injections at 4 months in all three subtypes. However, a rebound increase in volume was noticed in both occult CNV and RAP cohort at 12 months despite the initial, steeper reductions in the subtypes. These findings were consistent with the data seen in volume measurement using Topcon’s automated segmentation algorithm in a smaller cohort of patients. We propose that SHRM should be used as a potential biomarker to quantify both disease progression and prognosis of neovascular ARMD alongside other conventional methods.
在光学相干断层扫描(OCT)上看到的视网膜下超反射物质(SHRM)被认为是在年龄相关性黄斑变性(ARMD)中发现的纤维组织和血管网络的集合。我们对91例新血管性ARMD亚型的OCT扫描进行了回顾性分析,包括经典和隐匿脉络膜新生血管(CNV)和视网膜血管瘤增生(RAP)。所有三种亚型患者在负荷剂量后根据需要接受雷尼单抗,一种抗血管内皮生长因子(Anti-VEGF)玻璃体内注射。使用卡尺测量oct上看到的最大高度和最大长度来计算SHRM的体积。使用由肿瘤模型导出的椭球公式来计算体积。发现隐蔽CNV和RAP的SHRM体积大于经典CNV。在所有三种亚型中,在4个月时抗vegf注射负荷剂量后,SHRM体积总体上减少。然而,在隐匿性CNV和RAP组中,尽管亚型最初的急剧减少,但在12个月时均注意到体积的反弹增加。这些发现与在一个较小的患者队列中使用Topcon的自动分割算法进行体积测量的数据一致。我们建议SHRM应该作为一种潜在的生物标志物,与其他传统方法一起量化新血管性ARMD的疾病进展和预后。
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引用次数: 0
Resolution dependence on phase extraction by the Hilbert transform in phase calibrated and dispersion compensated ultrahigh resolution spectrometer-based OCT 基于相位校准和色散补偿的超高分辨率OCT中希尔伯特变换相位提取对分辨率的依赖
N. Israelsen, M. Maria, T. Feuchter, A. Bradu, A. Podoleanu, O. Bang
Ultrahigh resolution optical coherence tomography (UHR-OCT) is enabled by using a broad band source. Simultaneously, this makes the OCT image more sensitive to dispersion mismatch in the interferometer. In spectral domain OCT, dispersion left uncompensated in the interferometer and detector non-linearities lead together to an unknown chirp of the detected interferogram. One method to compensate for the chirp is to perform a pixel-wavenumber calibration versus phase that requires numerical extraction of the phase. Typically a Hilbert transform algorithm is employed to extract the optical phase versus wavenumber for calibration and dispersion compensation. In this work we demonstrate UHR-OCT at 1300 nm using a Super continuum source and highlight the resolution constraints in using the Hilbert transform algorithm when extracting the optical phase for calibration and dispersion compensation. We demonstrate that the constraints cannot be explained purely by the numerical errors in the data processing module utilizing the Hilbert transform but must be dictated by broadening mechanisms originating from the experimentally obtained interferograms.
超高分辨率光学相干层析成像(UHR-OCT)通过使用宽带源实现。同时,这使得OCT图像对干涉仪中的色散失配更加敏感。在光谱域OCT中,在干涉仪和检测器的非线性中未补偿的色散导致被检测干涉图的未知啁啾。补偿啁啾的一种方法是对相位进行像素-波数校准,这需要对相位进行数值提取。通常采用希尔伯特变换算法提取光相位与波数,用于校准和色散补偿。在这项工作中,我们展示了1300 nm下使用超连续介质源的UHR-OCT,并强调了在提取光学相位用于校准和色散补偿时使用希尔伯特变换算法的分辨率限制。我们证明,这些约束不能纯粹由利用希尔伯特变换的数据处理模块中的数值误差来解释,而必须由来自实验获得的干涉图的展宽机制来决定。
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引用次数: 0
Dental impression technique using optoelectronic devices 利用光电器件的牙印技术
C. Sinescu, Souman Barua, F. Topala, M. Negruțiu, Virgil-Florin Duma, A. Gabor, C. Zaharia, A. Bradu, A. Podoleanu
INTRODUCTION: The use of Optical Coherence Tomography (OCT) as a non-invasive and high precision quantitative information providing tool has been well established by researches within the last decade. The marginal discrepancy values can be scrutinized in optical biopsy made in three dimensional (3D) micro millimetre scale and reveal detailed qualitative and quantitative information of soft and hard tissues. OCT-based high resolution 3D images can provide a significant impact on finding recurrent caries, restorative failure, analysing the precision of crown preparation, and prosthetic elements marginal adaptation error with the gingiva and dental hard tissues. During the CAD/CAM process of prosthodontic restorations, the circumvent of any error is important for the practitioner and the technician to reduce waste of time and material. Additionally, OCT images help to achieve a new or semi-skilled practitioner to analyse their crown preparation works and help to develop their skills faster than in a conventional way. The aim of this study is to highlight the advantages of OCT in high precision prosthodontic restorations. MATERIALS AND METHODS: 25 preparations of frontal and lateral teeth were performed for 7 different patients. The impressions of the prosthetic fields were obtained both using a conventional optoelectronic system (Apolo Di, Syrona) and a Spectral Domain using OCT (Dental prototype, working at 860 nm). For the conventional impression technique the preparation margins were been prelevated by gingival impregnated cords. No specific treatments were performed by the OCT impression technique. RESULTS: The scanning performed by conventional optoelectronic system proved to be quick and accurate in terms of impression technology. The results were represented by 3D virtual models obtained after the scanning procedure was completed. In order to obtain a good optical impression a gingival retraction cord was inserted between the prepared tooth and the gingival tissue for a better elevation of the tooth cervical margin preparation. Spectral OCT was enforced in order to observe the quality but also the advantages coming from this technology. No special preparation was performed for this operation. CONCLUSION: Considering these aspects, OCT could be used as a valuable tool for dental impression technology, being non-invasive but also non-destructive on the marginal gingival tissue, in comparison with conventional optoelectronic technology where the gingival retraction cord is still mandatory.
光学相干层析成像(OCT)作为一种非侵入性和高精度的定量信息提供工具,在过去十年的研究中得到了很好的应用。在三维(3D)微毫米尺度的光学活检中,可以仔细检查边缘差异值,并揭示软硬组织的详细定性和定量信息。基于oct的高分辨率三维图像对发现复发性龋、修复失败、分析冠制备精度、修复体与牙龈和牙体硬组织的边缘适应误差具有重要意义。在修复体的CAD/CAM过程中,为了减少时间和材料的浪费,避免任何错误对于医生和技术人员来说都是非常重要的。此外,OCT图像有助于新手或半熟练的从业者分析他们的冠准备工作,并有助于比传统方法更快地发展他们的技能。本研究的目的是强调OCT在高精度修复中的优势。材料与方法:对7例患者进行前牙和侧牙的25个预备。使用传统光电系统(Apolo Di, Syrona)和使用OCT(牙科原型,工作在860 nm)的光谱域获得假体场的印象。传统的印模技术是用浸渍牙龈的软索来抬高印模边缘。未采用OCT压印技术进行特异性治疗。结果:传统光电扫描系统在印模技术方面具有快速、准确的特点。结果由扫描过程完成后获得的三维虚拟模型表示。为了获得良好的光学印象,在预备的牙齿和牙龈组织之间插入一根牙龈牵索,以更好地抬高牙颈缘预备。为了观察质量和该技术的优势,实施了光谱OCT。本次手术未做任何特殊准备。结论:考虑到这些方面,OCT可以作为牙印技术的一种有价值的工具,与传统的光电技术相比,它对牙龈边缘组织无创且无破坏性。
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引用次数: 1
Fast detection of vascular plaque in optical coherence tomography images using a reduced feature set 利用简化特征集快速检测光学相干断层扫描图像中的血管斑块
A. Prakash, Mariano Ocana Macias, M. Hewko, M. Sowa, S. Sherif
Optical coherence tomography (OCT) images are capable of detecting vascular plaque by using the full set of 26 Haralick textural features and a standard K-means clustering algorithm. However, the use of the full set of 26 textural features is computationally expensive and may not be feasible for real time implementation. In this work, we identified a reduced set of 3 textural feature which characterizes vascular plaque and used a generalized Fuzzy C-means clustering algorithm. Our work involves three steps: 1) the reduction of a full set 26 textural feature to a reduced set of 3 textural features by using genetic algorithm (GA) optimization method 2) the implementation of an unsupervised generalized clustering algorithm (Fuzzy C-means) on the reduced feature space, and 3) the validation of our results using histology and actual photographic images of vascular plaque. Our results show an excellent match with histology and actual photographic images of vascular tissue. Therefore, our results could provide an efficient pre-clinical tool for the detection of vascular plaque in real time OCT imaging.
光学相干断层扫描(OCT)图像能够通过使用全套26个哈拉里克纹理特征和标准k均值聚类算法来检测血管斑块。然而,使用完整的26个纹理特征集在计算上是昂贵的,并且可能不适合实时实现。在这项工作中,我们确定了一组简化的3个纹理特征来表征血管斑块,并使用了广义模糊c均值聚类算法。我们的工作包括三个步骤:1)使用遗传算法(GA)优化方法将完整的26个纹理特征约简为3个纹理特征的约简集;2)在约简特征空间上实现无监督广义聚类算法(Fuzzy C-means); 3)使用血管斑块的组织学和实际摄影图像验证我们的结果。我们的研究结果与维管组织的组织学和实际摄影图像非常吻合。因此,我们的研究结果可以为实时OCT成像中血管斑块的检测提供一种有效的临床前工具。
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引用次数: 2
OCT-based angiography in real time with hand-held probe 手持探头实时ct血管造影
G. Gelikonov, A. Moiseev, S. Ksenofontov, D. A. Terpelov, V. Gelikonov
This work is dedicated to development of the OCT system capable to visualize blood vessel network for everyday clinical use. Following problems were solved during the development: compensation of specific natural tissue displacements, induced by contact scanning mode and physiological motion of patients (e.g. respiratory and cardiac motions) and on-line visualization of vessel net to provide the feedback for system operator.
这项工作是致力于OCT系统的发展,能够可视化血管网络的日常临床使用。在开发过程中解决了以下问题:补偿由接触扫描模式引起的特定自然组织位移和患者的生理运动(如呼吸和心脏运动),以及血管网的在线可视化,为系统操作员提供反馈。
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引用次数: 1
Noise study of all-normal dispersion supercontinuum sources for potential application in optical coherence tomography 在光学相干层析成像中潜在应用的全正规色散超连续源的噪声研究
I. B. Gonzalo, R. D. Engelsholm, O. Bang
Commercially available silica-fiber-based and ultra-broadband supercontinuum (SC) sources are typically generated by pumping close to the zero-dispersion wavelength (ZDW) of a photonic crystal fiber (PCF), using high-power picosecond or nanosecond laser pulses. Despite the extremely broad bandwidths, such sources are characterized by large intensity fluctuations, limiting their performance for applications in imaging such as optical coherence tomography (OCT). An approach to eliminate the influence of noise sensitive effects is to use a so-called all-normal dispersion (ANDi) fiber, in which the dispersion is normal for all the wavelengths of interest. Pumping these types of fibers with short enough femtosecond pulses allows to suppress stimulated Raman scattering (SRS), which is known to be as noisy process as modulation instability (MI), and coherent SC is generated through self-phase modulation (SPM) and optical wave breaking (OWB). In this study, we show the importance of the pump laser and fiber parameters in the design of low-noise ANDi based SC sources, for application in OCT. We numerically investigate the pulse-to-pulse fluctuations of the SC, calculating the relative intensity noise (RIN) as a function of the pump pulse duration and fiber length. Furthermore, we experimentally demonstrate the role of the fiber length on the RIN of the ANDi SC, validating the results calculated numerically. In the end, we compare the RIN of a commercial SC source based on MI and the ANDi SC source developed here, which shows better noise performance when it is carefully designed.
商用硅纤维和超宽带超连续介质(SC)源通常是通过使用高功率皮秒或纳秒激光脉冲泵浦光子晶体光纤(PCF)的零色散波长(ZDW)附近产生的。尽管带宽非常宽,但这种光源的特点是强度波动大,限制了它们在光学相干断层扫描(OCT)等成像应用中的性能。消除噪声敏感效应影响的一种方法是使用所谓的全正常色散(ANDi)光纤,其中色散对所有感兴趣的波长都是正常的。用足够短的飞秒脉冲泵送这些类型的光纤,可以抑制受激拉曼散射(SRS),这是一个众所周知的与调制不稳定性(MI)一样的噪声过程,并且通过自相位调制(SPM)和光波破缺(OWB)产生相干SC。在这项研究中,我们展示了泵浦激光器和光纤参数在设计低噪声基于ANDi的SC源中的重要性,用于oct。我们数值研究了SC的脉冲对脉冲波动,计算了相对强度噪声(RIN)作为泵浦脉冲持续时间和光纤长度的函数。此外,我们通过实验证明了光纤长度对ANDi SC的RIN的作用,验证了数值计算的结果。最后,我们比较了基于MI的商用SC源和本文开发的ANDi SC源的RIN,后者在精心设计时表现出更好的噪声性能。
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引用次数: 4
Low-noise supercontinuum sources based on all-normal dispersion fibers: exploring their prospects and limitations 基于全正色散光纤的低噪声超连续声源:探讨其前景和局限性
A. Heidt, T. Feurer
Supercontinuum (SC) sources based on optical fibers exhibiting flat and normal group velocity dispersion (GVD) in the entire wavelength region of interest, so-called all-normal dispersion (ANDi) fibers, have become a well-accepted alternative to the more conventional approach of pumping fibers in the anomalous GVD region. The conservation of a single ultrashort pulse and the suppression of noise-amplifying nonlinear effects during SC generation make ANDi fibers particularly attractive for ultrafast and noise-sensitive applications. Here we discuss current possibilities and future prospects, but also the limitations of ANDi fiber SC sources, focusing particularly on coherence and noise performance for various pump pulse regimes.
基于在整个波长范围内表现出平坦和正常群速度色散(GVD)的光纤的超连续谱(SC)光源,即所谓的全正常色散(ANDi)光纤,已经成为一种被广泛接受的替代方法,以取代在异常GVD区域泵送光纤的更传统方法。在SC产生过程中,单超短脉冲的守恒和对噪声放大非线性效应的抑制使得ANDi光纤对超快和噪声敏感的应用特别有吸引力。在这里,我们讨论了当前的可能性和未来的前景,以及ANDi光纤SC源的局限性,特别关注各种泵浦脉冲制度的相干性和噪声性能。
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引用次数: 0
期刊
Canterbury Workshop and School in Optical Coherence Tomography and Adaptive Optics
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