Pub Date : 2020-08-31DOI: 10.3329/AAJBB.V5I2.53865
I. Jahan, A. A. Ahsan, M. Jahangir, M. Hossain, A. Abedin
Soil physico-chemical properties are an important phenomenon for sustainable crop production and maintenance of optimum soil health. Hence, a laboratory measurement was conducted with soil samples of three years long experimental field of the Department of Soil Science, Bangladesh Agricultural University, Mymensingh to assess the changes in five selected soil physico-chemical properties viz. soil texture, bulk density, soil pH, total nitrogen and organic matter. The experiment was laid out in a split plot design with two water regimes (continuous flooding and alternate wetting & drying) in the main plots and five fertilizer treatments (N0 - control, N1- 140 kg N/ha as PU, N2- 104 kg N/ha as USG (2× 1.8 g/ 4 hills), N3 - 5 t CD + PU @ 140 kg N /ha on IPNS basis and N4- 5 t CD + USG (2× 1.8 g/ 4 hills @ 104 kg N/ha)) in the subplots under rice-rice cropping pattern with three replications. After three years, soil samples were collected at 0-5 and 5-10 cm soil depths for measuring bulk density and at 0-10 cm depth for other soil properties and analyzed. Results found that % sand, % silt, % clay, bulk density and soil pH was not changed significantly compared to initial status. Percentage of total nitrogen and organic matter was significantly affected by irrigation and fertilization. Total nitrogen (%) was higher in AWD whereas organic matter (%) was higher in CF practice. The highest total nitrogen (%) and organic matter (%) was found in N4 treatment in which USG was applied in combination with cowdung as organic manure. It can be suggested that N4 treatment was formed good combination for sustaining chemical properties of soil. Further long- term experimentation will be needed to know the changes in soil properties for sustainable crop production and improving soil health. Asian Australas. J. Biosci. Biotechnol. 2020, 5 (2), 65-71
土壤理化性质是影响作物可持续生产和维持土壤最佳健康的重要因素。因此，对孟加拉国农业大学Mymensingh土壤科学系长达三年的实验田的土壤样本进行了实验室测量，以评估土壤五种选定的土壤理化性质的变化，即土壤质地、容重、土壤pH、全氮和有机质。实验是在分裂阴谋设计有两个水政权(连续洪水和备用湿润和干燥)的主要情节和五个施肥处理(N0——控制,N1 - 140公斤N /公顷PU、N2 - 104公斤N /公顷USG(2×1.8 g / 4山),N3 - 5 t CD + PU @施用肥料的基础上140公斤N /公顷和陶瓷- 5 t CD + USG(2×1.8 g / 4山@ 104公斤N /公顷))下的次要情节rice-rice种植模式与三个复制。三年后，在0-5 cm和5-10 cm土壤深度采集土壤样品用于测量容重，在0-10 cm土壤深度采集其他土壤特性并进行分析。结果发现，与初始状态相比，%砂、%粉、%粘土、容重和土壤pH值变化不显著。灌溉和施肥对全氮和有机质含量有显著影响。全氮(%)较高，而有机肥(%)较高。全氮(%)和有机质(%)以氮肥与牛粪配施为有机肥的处理最高。说明氮肥处理是维持土壤化学性质的良好组合。需要进一步的长期试验来了解土壤性质的变化，以促进可持续作物生产和改善土壤健康。亚洲Australas。j . Biosci。生物技术，2020,5 (2)，65-71
{"title":"Changes in physico-chemical properties of paddy soil due to water and fertilizer management","authors":"I. Jahan, A. A. Ahsan, M. Jahangir, M. Hossain, A. Abedin","doi":"10.3329/AAJBB.V5I2.53865","DOIUrl":"https://doi.org/10.3329/AAJBB.V5I2.53865","url":null,"abstract":"Soil physico-chemical properties are an important phenomenon for sustainable crop production and maintenance of optimum soil health. Hence, a laboratory measurement was conducted with soil samples of three years long experimental field of the Department of Soil Science, Bangladesh Agricultural University, Mymensingh to assess the changes in five selected soil physico-chemical properties viz. soil texture, bulk density, soil pH, total nitrogen and organic matter. The experiment was laid out in a split plot design with two water regimes (continuous flooding and alternate wetting & drying) in the main plots and five fertilizer treatments (N0 - control, N1- 140 kg N/ha as PU, N2- 104 kg N/ha as USG (2× 1.8 g/ 4 hills), N3 - 5 t CD + PU @ 140 kg N /ha on IPNS basis and N4- 5 t CD + USG (2× 1.8 g/ 4 hills @ 104 kg N/ha)) in the subplots under rice-rice cropping pattern with three replications. After three years, soil samples were collected at 0-5 and 5-10 cm soil depths for measuring bulk density and at 0-10 cm depth for other soil properties and analyzed. Results found that % sand, % silt, % clay, bulk density and soil pH was not changed significantly compared to initial status. Percentage of total nitrogen and organic matter was significantly affected by irrigation and fertilization. Total nitrogen (%) was higher in AWD whereas organic matter (%) was higher in CF practice. The highest total nitrogen (%) and organic matter (%) was found in N4 treatment in which USG was applied in combination with cowdung as organic manure. It can be suggested that N4 treatment was formed good combination for sustaining chemical properties of soil. Further long- term experimentation will be needed to know the changes in soil properties for sustainable crop production and improving soil health. \u0000Asian Australas. J. Biosci. Biotechnol. 2020, 5 (2), 65-71","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129712709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.3329/aajbb.v4i3.65083
Screening of salinity tolerance genotypes of rice on the basis of its phenotypic performance alone is not much reliable and will take more time in progress in breeding process. Molecular marker-based screening eases this process. An experiment was carried out with 22 diverse rice genotypes in Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh to study their salt tolerance at both seedling and reproductive stages. On the basis of yield and yield contributing traits, genotypes were categorized as tolerant, moderately tolerant and susceptible. The genotypes RC 227, RC 229, RC 191, Binadhan-8, Binadhan-10, Binadhan-11 and FL-478 were found as tolerant, while Binadhan-7 and BRRI dhan39 were found as susceptible. Grain weight hill-1 was found highly significant. Plant height, total tiller hill-1, grain weight panicle-1, 1000 grain weight and all other traits except sterile spikelets panicle-1 were showed a positive correlation with grain weight hill-1. Selected four salt linked SSR markers viz. AP3206f, RM1287, RM7075 and RM10793 were used to determine salinity tolerance. The genetic diversity was ranges from 0.6116 to 0.7810 with an average of 0.6663. The highest PIC value was 0.7524 and the lowest was 0.4762 from AP3206f and RM7075, respectively. The UPGMA clustering system generated four genetic clusters. The highest genetically dissimilarity of (Cluster 1) vs (Cluster 3) and the crossing would be helpful for salt tolerant rice development. Thus, selected SSR primers and genotypes would be useful in marker assisted selection (MAS), quantitative trait loci (QTL) mapping, gene pyramiding and ultimately improvement of salt tolerant rice varieties. Asian Australas. J. Biosci. Biotechnol. 2019, 4 (3), 176-183
{"title":"Assessment of rice genotypes for salt stress at seedling and reproductive stage by using phenotypic and molecular markers","authors":"","doi":"10.3329/aajbb.v4i3.65083","DOIUrl":"https://doi.org/10.3329/aajbb.v4i3.65083","url":null,"abstract":"Screening of salinity tolerance genotypes of rice on the basis of its phenotypic performance alone is not much reliable and will take more time in progress in breeding process. Molecular marker-based screening eases this process. An experiment was carried out with 22 diverse rice genotypes in Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh to study their salt tolerance at both seedling and reproductive stages. On the basis of yield and yield contributing traits, genotypes were categorized as tolerant, moderately tolerant and susceptible. The genotypes RC 227, RC 229, RC 191, Binadhan-8, Binadhan-10, Binadhan-11 and FL-478 were found as tolerant, while Binadhan-7 and BRRI dhan39 were found as susceptible. Grain weight hill-1 was found highly significant. Plant height, total tiller hill-1, grain weight panicle-1, 1000 grain weight and all other traits except sterile spikelets panicle-1 were showed a positive correlation with grain weight hill-1. Selected four salt linked SSR markers viz. AP3206f, RM1287, RM7075 and RM10793 were used to determine salinity tolerance. The genetic diversity was ranges from 0.6116 to 0.7810 with an average of 0.6663. The highest PIC value was 0.7524 and the lowest was 0.4762 from AP3206f and RM7075, respectively. The UPGMA clustering system generated four genetic clusters. The highest genetically dissimilarity of (Cluster 1) vs (Cluster 3) and the crossing would be helpful for salt tolerant rice development. Thus, selected SSR primers and genotypes would be useful in marker assisted selection (MAS), quantitative trait loci (QTL) mapping, gene pyramiding and ultimately improvement of salt tolerant rice varieties. \u0000Asian Australas. J. Biosci. Biotechnol. 2019, 4 (3), 176-183","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115665785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.3329/aajbb.v4i3.65074
Most Jharna Khatun, Subrata Mondal
An investigation was carried out to provide an initial assessment of aquarium fish trade from December 2018 to May 2019 in Khulna city, Khulna of Bangladesh. A total 12 shops were established in this district where 46 aquarium fish species were found of which 12 species were bred for fry production. In the study area, some ornamental fishes such as Gold fish, Black moor, Comet, Red parrot, Tiger barb, Angel fish, Black molly, Guppy, koi carp, Rosy barb, Silver shark, Rainbow shark, Tiger shark, Sucker mouth catfish, Blue gourami, Pearl gourami, Golden gourami, White molly, Pearl scale gold fish, Silver dollar, LT sucker fish were very common. Besides Glass catfish, Siamese fighting fish, calico, Swordtail, Oscar, Widow tetra, Neon tetra, Balloon molly, Moon tail balloon molly, Alligator gar were common and Tangerrine swordtail, Arowana, Flower horn, Red tail black shark, platy, cardinal tetra, Discuss fish, Rummy nose, Bolivian ram, Balloon ram, Bosemoni rainbow, Red tail catfish, Bichir, Mono angel, Knife fish were the rare fish of the Khulna city. All aquarium fish species were brought from catabon area of Dhaka city that were mainly imported from the international markets like India, Singapore, Thailand, etc. Importers/wholesalers and breeders marketed them to retailers, local breeders, shopkeepers, etc. and finally, the shopkeepers sold them to the aquaria owners. The most profitable large aquarium fishes were Gold fish which made a profit of (100±20 tk/pair), Black moor (150±20 tk/pair), Red parrot (250±50 tk/pair), angelfish (100±10 tk/pair), Pearl gourami (300±50 tk/pair), Guppy (400±50 tk/pair), Siamese Fighting Fish (300±50 tk /piece), Tiger Shark (40±10 tk/pair), Sucker mouth catfish (50±5 tk/pair) to the retailers. During this survey, it was found that the breeders used cement tank, glass aquaria, plastic tank and even plastic jar as culture tanks. They bred aquarium fish all over the year. In the present study the market is mainly domestic. There is a good domestic market which is increasing day by day. Except winter, average annual income was recorded 13,140,000 Tk from the 12 aquarium shops. However, when temperature dropped during winter, sale became reduced; sometimes no selling was also observed during this time. Asian Australas. J. Biosci. Biotechnol. 2019, 4 (3), 142-153
{"title":"Ornamental fishes in Khulna City, Bangladesh: culture practices and commercial aspects","authors":"Most Jharna Khatun, Subrata Mondal","doi":"10.3329/aajbb.v4i3.65074","DOIUrl":"https://doi.org/10.3329/aajbb.v4i3.65074","url":null,"abstract":"An investigation was carried out to provide an initial assessment of aquarium fish trade from December 2018 to May 2019 in Khulna city, Khulna of Bangladesh. A total 12 shops were established in this district where 46 aquarium fish species were found of which 12 species were bred for fry production. In the study area, some ornamental fishes such as Gold fish, Black moor, Comet, Red parrot, Tiger barb, Angel fish, Black molly, Guppy, koi carp, Rosy barb, Silver shark, Rainbow shark, Tiger shark, Sucker mouth catfish, Blue gourami, Pearl gourami, Golden gourami, White molly, Pearl scale gold fish, Silver dollar, LT sucker fish were very common. Besides Glass catfish, Siamese fighting fish, calico, Swordtail, Oscar, Widow tetra, Neon tetra, Balloon molly, Moon tail balloon molly, Alligator gar were common and Tangerrine swordtail, Arowana, Flower horn, Red tail black shark, platy, cardinal tetra, Discuss fish, Rummy nose, Bolivian ram, Balloon ram, Bosemoni rainbow, Red tail catfish, Bichir, Mono angel, Knife fish were the rare fish of the Khulna city. All aquarium fish species were brought from catabon area of Dhaka city that were mainly imported from the international markets like India, Singapore, Thailand, etc. Importers/wholesalers and breeders marketed them to retailers, local breeders, shopkeepers, etc. and finally, the shopkeepers sold them to the aquaria owners. The most profitable large aquarium fishes were Gold fish which made a profit of (100±20 tk/pair), Black moor (150±20 tk/pair), Red parrot (250±50 tk/pair), angelfish (100±10 tk/pair), Pearl gourami (300±50 tk/pair), Guppy (400±50 tk/pair), Siamese Fighting Fish (300±50 tk /piece), Tiger Shark (40±10 tk/pair), Sucker mouth catfish (50±5 tk/pair) to the retailers. During this survey, it was found that the breeders used cement tank, glass aquaria, plastic tank and even plastic jar as culture tanks. They bred aquarium fish all over the year. In the present study the market is mainly domestic. There is a good domestic market which is increasing day by day. Except winter, average annual income was recorded 13,140,000 Tk from the 12 aquarium shops. However, when temperature dropped during winter, sale became reduced; sometimes no selling was also observed during this time. \u0000Asian Australas. J. Biosci. Biotechnol. 2019, 4 (3), 142-153","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127341824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.3329/aajbb.v4i3.65072
S. Kabir
An investigation was carried out on fish market infrastructure of Khulna district to identify the infrastructure facilities of domestic fish market including species traded along with price and also to detect the constraints at different levels of trading recommending possible solutions according to standards. The investigation was conducted from November 2016 to June 2017 in three different markets of Khulna district namely Sandhya Bazar fish market, Aranghata Bypass Bazar fish market and Baikali Bazar fish market. In Khulna, a large number of people are involved in fish marketing. Market infrastructures of Khulna had grown up according to various influencing factors that controls the total system involved. The study was based on observation and field survey. Data were collected through eye observation, questionnaire interviews and focus group discussions. Several number of market agents including wholesalers, retailers were selected for questionnaire interviews and aratdars and fishermen were selected for focused group discussions. It was revealed that the infrastructure facilities were inadequate and poorly maintained. Fish sheds were not enough hygienic to protect the fish from contamination. Surface water drainage system was open and this situation degraded further environmental condition of the markets. Electricity maintenance was not up to the mark and there were no distillation process for the supplied water. This infrastructural condition does not support a hygienic condition for fish marketing to provide edible and quality fish. Total number of freshwater fish species traded in different markets was found 60 including 12 SIS, 34 culture and 14 capture. On the other hand 41 brackish and marine water species were found and 14 crustacean species of which 5 were freshwater prawn, 6 brackish water shrimp and 3 crabs. Therefore domestic market infrastructure is on a developing stage as being controlled by Khulna City Corporation (KCC). Establishment of ice factory, improvement of fish transport facilities, introduction of fish quality control measures were suggested to improve the fish marketing in the study area. Asian Australas. J. Biosci. Biotechnol. 2020, 5 (1), 122-134
{"title":"Fish market infrastructure in Khulna city, Bangladesh","authors":"S. Kabir","doi":"10.3329/aajbb.v4i3.65072","DOIUrl":"https://doi.org/10.3329/aajbb.v4i3.65072","url":null,"abstract":"An investigation was carried out on fish market infrastructure of Khulna district to identify the infrastructure facilities of domestic fish market including species traded along with price and also to detect the constraints at different levels of trading recommending possible solutions according to standards. The investigation was conducted from November 2016 to June 2017 in three different markets of Khulna district namely Sandhya Bazar fish market, Aranghata Bypass Bazar fish market and Baikali Bazar fish market. In Khulna, a large number of people are involved in fish marketing. Market infrastructures of Khulna had grown up according to various influencing factors that controls the total system involved. The study was based on observation and field survey. Data were collected through eye observation, questionnaire interviews and focus group discussions. Several number of market agents including wholesalers, retailers were selected for questionnaire interviews and aratdars and fishermen were selected for focused group discussions. It was revealed that the infrastructure facilities were inadequate and poorly maintained. Fish sheds were not enough hygienic to protect the fish from contamination. Surface water drainage system was open and this situation degraded further environmental condition of the markets. Electricity maintenance was not up to the mark and there were no distillation process for the supplied water. This infrastructural condition does not support a hygienic condition for fish marketing to provide edible and quality fish. Total number of freshwater fish species traded in different markets was found 60 including 12 SIS, 34 culture and 14 capture. On the other hand 41 brackish and marine water species were found and 14 crustacean species of which 5 were freshwater prawn, 6 brackish water shrimp and 3 crabs. Therefore domestic market infrastructure is on a developing stage as being controlled by Khulna City Corporation (KCC). Establishment of ice factory, improvement of fish transport facilities, introduction of fish quality control measures were suggested to improve the fish marketing in the study area. Asian Australas. J. Biosci. Biotechnol. 2020, 5 (1), 122-134","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130684243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.3329/aajbb.v4i3.65075
M. Amin, Md. Mehedi Hasan Khokon, N. Akter, Md. Nurul Al Imran, M. J. Uddin, Md. Ahsanur Reza
The experiment was conducted in Department of Physiology and Pharmacology, FANSVM, PSTU to study the effects of seven indigenous medicinal plants [black pepper (Piper nirgum), tulsi (Ocimum sanctum), peppermint (Mentha piperita), garlic (Allium sativum), black cumin (Nigella sativa), papaya (Carica papaya), cinnamon (Cinnamomum verum)] and two patent probiotics (SS Bio® and Poultrystar Sol®) on the growth performance (body weight and FCR) in broiler. The effects of black pepper, tulsi, peppermint, garlic, black cumin, papaya, cinnamon, SS Bio® and Poultrystar Sol® on hematological parameters (TEC, Hb and PCV) and biochemical parameter (blood glucose) were also observed. One hundred broiler were randomly divided into ten groups (A, B, C, D, E, F, G, H, I and J), each consisting of ten broiler. Group A was kept as control. 10% water extract of black pepper (seed), tulsi (leaves), peppermint (leaves), garlic (bulbs), black cumin (seed), papaya (leaves), cinnamon (bark) were administered orally to the broiler of group B, C, D, E, F, G and H, respectively. Broiler of group I and J received orally SS Bio® and Poultrystar Sol®, respectively. Black pepper, tulsi, peppermint, garlic, black cumin, papaya, cinnamon, SS Bio® and Poultrystar Sol® were significantly (p<0.01 and p<0.05) increased body weight and FCR up to the experimental period. TEC was significantly (p<0.05) increased at garlic treated broiler. After treatment with peppermint, garlic, black cumin, papaya, cinnamon, SS Bio® and Poultrystar Sol®, Hb content increased significantly (p<0.05) in broiler. Black pepper, tulsi, peppermint, black cumin, papaya, SS Bio® and Poultrystar Sol® treatment significantly (p<0.01 and p<0.05) increased PCV in broiler. Peppermint, garlic, black cumin, papaya, cinnamon, SS Bio® and Poultrystar Sol® caused significantly (p<0.01 and p<0.05) increased blood glucose. Tulsi decreased (p<0.05) significantly blood glucose. The present study reveal that indigenous medicinal plants can be used as alternatives to patent probiotics in broiler. Asian Australas. J. Biosci. Biotechnol. 2019, 4 (3), 135-141
{"title":"Effects of black pepper, tulsi, peppermint, garlic, black cumin, papaya, cinnamon and patent probiotics on growth performance of broiler","authors":"M. Amin, Md. Mehedi Hasan Khokon, N. Akter, Md. Nurul Al Imran, M. J. Uddin, Md. Ahsanur Reza","doi":"10.3329/aajbb.v4i3.65075","DOIUrl":"https://doi.org/10.3329/aajbb.v4i3.65075","url":null,"abstract":"The experiment was conducted in Department of Physiology and Pharmacology, FANSVM, PSTU to study the effects of seven indigenous medicinal plants [black pepper (Piper nirgum), tulsi (Ocimum sanctum), peppermint (Mentha piperita), garlic (Allium sativum), black cumin (Nigella sativa), papaya (Carica papaya), cinnamon (Cinnamomum verum)] and two patent probiotics (SS Bio® and Poultrystar Sol®) on the growth performance (body weight and FCR) in broiler. The effects of black pepper, tulsi, peppermint, garlic, black cumin, papaya, cinnamon, SS Bio® and Poultrystar Sol® on hematological parameters (TEC, Hb and PCV) and biochemical parameter (blood glucose) were also observed. One hundred broiler were randomly divided into ten groups (A, B, C, D, E, F, G, H, I and J), each consisting of ten broiler. Group A was kept as control. 10% water extract of black pepper (seed), tulsi (leaves), peppermint (leaves), garlic (bulbs), black cumin (seed), papaya (leaves), cinnamon (bark) were administered orally to the broiler of group B, C, D, E, F, G and H, respectively. Broiler of group I and J received orally SS Bio® and Poultrystar Sol®, respectively. Black pepper, tulsi, peppermint, garlic, black cumin, papaya, cinnamon, SS Bio® and Poultrystar Sol® were significantly (p<0.01 and p<0.05) increased body weight and FCR up to the experimental period. TEC was significantly (p<0.05) increased at garlic treated broiler. After treatment with peppermint, garlic, black cumin, papaya, cinnamon, SS Bio® and Poultrystar Sol®, Hb content increased significantly (p<0.05) in broiler. Black pepper, tulsi, peppermint, black cumin, papaya, SS Bio® and Poultrystar Sol® treatment significantly (p<0.01 and p<0.05) increased PCV in broiler. Peppermint, garlic, black cumin, papaya, cinnamon, SS Bio® and Poultrystar Sol® caused significantly (p<0.01 and p<0.05) increased blood glucose. Tulsi decreased (p<0.05) significantly blood glucose. The present study reveal that indigenous medicinal plants can be used as alternatives to patent probiotics in broiler. \u0000Asian Australas. J. Biosci. Biotechnol. 2019, 4 (3), 135-141","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133323419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.3329/aajbb.v4i3.65079
S. Pal, Jotirmoy Chakrobortty, M. Hashem, Md. Anamul Hoque
Appropriate nutrient management could increase crop productivity in saline areas by reducing the harmful effects of soil salinity. A field experiment was carried out at farmer’s field of Botiaghata, Khulna to investigate the mitigation of soil salinity by efficient management of potassium and zinc fertilizers in rice. BRRI dhan53, a salt-tolerant rice cultivar during aman season, was used as a test crop. The experiment was laid out in a randomized complete block design with three replications where sixteen treatment combinations were used in a factorial arrangement. Potassium and zinc nutrients were supplied from potassium sulphate and zinc sulphate, respectively and they were applied in two splits, first split during final land preparation and second split at maximum tillering stage. Recommended doses of N, P and S fertilizers were applied to all the experimental plots. Results revealed that plant height, panicle length, number of effective tillers and grains per panicle increased significantly due to application of K and Zn fertilizers. All the yield contributing parameters showed higher values in T15 (K200Zn200) treatment compared to other treatments. Grain and straw yields of BRRI dhan53 responded significantly to the different treatment combinations. The highest grain yield (4.46 t/ha) was obtained in T15 (K200Zn200) treatment and the highest straw yield (6.27 t/ha) was also obtained in the same treatment. Higher doses of potassium and zinc also increased nutrient uptake (N, P, S and Zn) by BRRI dhan53. The K+/Na+ ratio was also found higher in grain (0.319) and straw (0.301) in T15 treatment. Therefore, result of the experiment indicated that application of higher doses of K and Zn fertilizers could alleviate the adverse effects of salinity in rice production by increasing nutrient uptake and maintaining higher K+/Na+ ratio. Asian Australas. J. Biosci. Biotechnol. 2019, 4 (3), 154-161
{"title":"Improvement of salinity tolerance in rice by efficient management of potassium and zinc fertilizers","authors":"S. Pal, Jotirmoy Chakrobortty, M. Hashem, Md. Anamul Hoque","doi":"10.3329/aajbb.v4i3.65079","DOIUrl":"https://doi.org/10.3329/aajbb.v4i3.65079","url":null,"abstract":"Appropriate nutrient management could increase crop productivity in saline areas by reducing the harmful effects of soil salinity. A field experiment was carried out at farmer’s field of Botiaghata, Khulna to investigate the mitigation of soil salinity by efficient management of potassium and zinc fertilizers in rice. BRRI dhan53, a salt-tolerant rice cultivar during aman season, was used as a test crop. The experiment was laid out in a randomized complete block design with three replications where sixteen treatment combinations were used in a factorial arrangement. Potassium and zinc nutrients were supplied from potassium sulphate and zinc sulphate, respectively and they were applied in two splits, first split during final land preparation and second split at maximum tillering stage. Recommended doses of N, P and S fertilizers were applied to all the experimental plots. Results revealed that plant height, panicle length, number of effective tillers and grains per panicle increased significantly due to application of K and Zn fertilizers. All the yield contributing parameters showed higher values in T15 (K200Zn200) treatment compared to other treatments. Grain and straw yields of BRRI dhan53 responded significantly to the different treatment combinations. The highest grain yield (4.46 t/ha) was obtained in T15 (K200Zn200) treatment and the highest straw yield (6.27 t/ha) was also obtained in the same treatment. Higher doses of potassium and zinc also increased nutrient uptake (N, P, S and Zn) by BRRI dhan53. The K+/Na+ ratio was also found higher in grain (0.319) and straw (0.301) in T15 treatment. Therefore, result of the experiment indicated that application of higher doses of K and Zn fertilizers could alleviate the adverse effects of salinity in rice production by increasing nutrient uptake and maintaining higher K+/Na+ ratio. \u0000Asian Australas. J. Biosci. Biotechnol. 2019, 4 (3), 154-161","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"116 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124919690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.3329/aajbb.v4i3.65082
Thakurdas Roy, D. Bhusan, M. M. Rahman, Md. Anamul Hoque
Salinity is a major limiting factor reducing crop yields in coastal areas of Bangladesh. The main objective of this study was to mitigate the adverse effects of soil salinity on wheat by organic and inorganic amendments. The field experiment was conducted at BRRI station, Sonagazi, Feni. Wheat cv. BARI Gom-23 was used as a test crop. There were thirteen treatment combinations consisting of control, farmyard manure (FYM) (5 t ha-1), FYM (10 t ha-1), compost (10 t ha-1), compost (20 t ha-1), gypsum (50% GR), gypsum (100% GR), K2SO4 (100 kg ha-1), K2SO4 (200 kg ha-1), FYM (5 t ha-1) + gypsum (50% GR), FYM (5 t ha-1) + K2SO4 (100 kg ha-1), compost (10 t ha-1) + gypsum (50% GR), compost (10 t ha-1) + K2SO4 (100 kg ha-1). FYM and compost were added to the soils during final land preparation. Gypsum and sulphate of potash were applied in two splits as per treatments. Nitrogen, phosphorous, zinc and boron fertilizers were applied as basal doses. The experiments were laid out in a randomized complete block design with three replications. Soil salinity caused a reduction in growth and yield of wheat. Soil amendments with organic or inorganic fertilizers improved growth and yield of wheat under soil salinity. Combined application of organic and inorganic fertilizers showed higher yields of wheat than that of alone during salinity conditions. Both organic and inorganic fertilizers increased nutrient uptake and K+/Na+ ratio in wheat during salinity condition. Therefore, the present study suggests that wheat production might be feasible in coastal areas of southern Bangladesh through organic and inorganic amendments of saline soils. Asian Australas. J. Biosci. Biotechnol. 2019, 4 (3), 169-175
盐度是降低孟加拉国沿海地区作物产量的主要限制因素。本研究的主要目的是通过有机和无机改良剂减轻土壤盐分对小麦的不利影响。田间试验在芬尼Sonagazi BRRI站进行。小麦的简历。BARI gom23被用作试验作物。试验设对照、农家肥(FYM) (5 t ha-1)、FYM (10 t ha-1)、堆肥(10 t ha-1)、堆肥(20 t ha-1)、石膏(50% GR)、石膏(100% GR)、K2SO4 (100 kg ha-1)、FYM (5 t ha-1) +石膏(50% GR)、FYM (5 t ha-1) + K2SO4 (100 kg ha-1)、堆肥(10 t ha-1) +石膏(50% GR)、堆肥(10 t ha-1) + K2SO4 (100 kg ha-1) 13个处理组合。在最后的土地准备过程中向土壤中添加FYM和堆肥。石膏和硫酸钾按处理分两段施用。施氮、磷、锌、硼为基础剂量。实验采用完全随机区组设计，重复3次。土壤盐碱化导致小麦生长和产量下降。施用有机或无机肥料的土壤改良剂对土壤盐渍化条件下小麦的生长和产量有促进作用。在盐渍化条件下，有机无机肥配施小麦产量高于单施。盐胁迫下，有机肥和无机肥均能提高小麦的养分吸收和钾钠比。因此，本研究表明，在孟加拉国南部沿海地区，通过对盐渍土进行有机和无机修正，小麦生产可能是可行的。亚洲Australas。j . Biosci。生物技术学报，2019,4 (3)，169-175
{"title":"Enhancement of the growth and yield of wheat in coastal saline areas through organic and inorganic amendments","authors":"Thakurdas Roy, D. Bhusan, M. M. Rahman, Md. Anamul Hoque","doi":"10.3329/aajbb.v4i3.65082","DOIUrl":"https://doi.org/10.3329/aajbb.v4i3.65082","url":null,"abstract":"Salinity is a major limiting factor reducing crop yields in coastal areas of Bangladesh. The main objective of this study was to mitigate the adverse effects of soil salinity on wheat by organic and inorganic amendments. The field experiment was conducted at BRRI station, Sonagazi, Feni. Wheat cv. BARI Gom-23 was used as a test crop. There were thirteen treatment combinations consisting of control, farmyard manure (FYM) (5 t ha-1), FYM (10 t ha-1), compost (10 t ha-1), compost (20 t ha-1), gypsum (50% GR), gypsum (100% GR), K2SO4 (100 kg ha-1), K2SO4 (200 kg ha-1), FYM (5 t ha-1) + gypsum (50% GR), FYM (5 t ha-1) + K2SO4 (100 kg ha-1), compost (10 t ha-1) + gypsum (50% GR), compost (10 t ha-1) + K2SO4 (100 kg ha-1). FYM and compost were added to the soils during final land preparation. Gypsum and sulphate of potash were applied in two splits as per treatments. Nitrogen, phosphorous, zinc and boron fertilizers were applied as basal doses. The experiments were laid out in a randomized complete block design with three replications. Soil salinity caused a reduction in growth and yield of wheat. Soil amendments with organic or inorganic fertilizers improved growth and yield of wheat under soil salinity. Combined application of organic and inorganic fertilizers showed higher yields of wheat than that of alone during salinity conditions. Both organic and inorganic fertilizers increased nutrient uptake and K+/Na+ ratio in wheat during salinity condition. Therefore, the present study suggests that wheat production might be feasible in coastal areas of southern Bangladesh through organic and inorganic amendments of saline soils. \u0000Asian Australas. J. Biosci. Biotechnol. 2019, 4 (3), 169-175","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128224100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.3329/aajbb.v4i3.65081
M. Islam, Md. Shajedur Rahman, M. Z. Hossain, M. Haque
The present study was carried out to detect the prevalence of anaplasmosis in cattle at Rayganj and Ullahpara upazila in Sirajganj district of Bangladesh and to evaluate the therapeutic efficacy of traditional drugs like Oxetetracycline (Renamycine®), Imidocarb diproprionate (Babenil®) and Diminazine aceturate (Berenil®) against anaplasmosis in cattle. The study was conducted from July 2016 to December 2016. During six months of study period a total of 150 blood samples were collected from clinically ill and suspected cattle, among which 28 samples were positive for anaplasmosis by Geimsa stained blood smear method. It was observed that the overall prevalence of anaplasmosis in cattle was 18.67%, where 16.25% was Geimsa stained blood smear at Rayganj and 21.43% at Ullapara upazila respectively and the variation was not statistically significant (p>0.05). In respect of age the prevalence of anaplasmosis was significantly (p< 0.05) higher (31.7%) in 2-3 years of age group cattle than above 3 years (16.67%) and 6 months to 2 years age (10.20%) group. On the basis of sex, it was observed that the variation in prevalence in male (15.94%) and female (20.99%) was not statistically significant (p>0.05). Breed-wise prevalence was higher in crossbred cattle (19.5%) than local cattle (17.8%), was not statistically significant (p>0.05). It was observed that among three drugs used in this study, the best effectiveness of drugs was seen by Oxytetracycline (Renamycine LA®) @ 10 mg/kg body weight followed by Imidocarb dipropionate (Babenil®) @ 3.5 mg/kg body weight moderately and less by Diminazine aceturate (Berenil®) @ 3.5 mg/kg body weight. From the study it was evident that cattle were infected with the organisms and caused a heavy economic loss which recommended taking necessary preventive measurements. Asian Australas. J. Biosci. Biotechnol. 2019, 4 (3), 162-168
{"title":"Emerging status of anaplasmosis in cattle in Sirajganj district with therapeutic evaluation of traditional treatments","authors":"M. Islam, Md. Shajedur Rahman, M. Z. Hossain, M. Haque","doi":"10.3329/aajbb.v4i3.65081","DOIUrl":"https://doi.org/10.3329/aajbb.v4i3.65081","url":null,"abstract":"The present study was carried out to detect the prevalence of anaplasmosis in cattle at Rayganj and Ullahpara upazila in Sirajganj district of Bangladesh and to evaluate the therapeutic efficacy of traditional drugs like Oxetetracycline (Renamycine®), Imidocarb diproprionate (Babenil®) and Diminazine aceturate (Berenil®) against anaplasmosis in cattle. The study was conducted from July 2016 to December 2016. During six months of study period a total of 150 blood samples were collected from clinically ill and suspected cattle, among which 28 samples were positive for anaplasmosis by Geimsa stained blood smear method. It was observed that the overall prevalence of anaplasmosis in cattle was 18.67%, where 16.25% was Geimsa stained blood smear at Rayganj and 21.43% at Ullapara upazila respectively and the variation was not statistically significant (p>0.05). In respect of age the prevalence of anaplasmosis was significantly (p< 0.05) higher (31.7%) in 2-3 years of age group cattle than above 3 years (16.67%) and 6 months to 2 years age (10.20%) group. On the basis of sex, it was observed that the variation in prevalence in male (15.94%) and female (20.99%) was not statistically significant (p>0.05). Breed-wise prevalence was higher in crossbred cattle (19.5%) than local cattle (17.8%), was not statistically significant (p>0.05). It was observed that among three drugs used in this study, the best effectiveness of drugs was seen by Oxytetracycline (Renamycine LA®) @ 10 mg/kg body weight followed by Imidocarb dipropionate (Babenil®) @ 3.5 mg/kg body weight moderately and less by Diminazine aceturate (Berenil®) @ 3.5 mg/kg body weight. From the study it was evident that cattle were infected with the organisms and caused a heavy economic loss which recommended taking necessary preventive measurements. \u0000Asian Australas. J. Biosci. Biotechnol. 2019, 4 (3), 162-168","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"176 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132789741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-31DOI: 10.3329/aajbb.v4i2.65071
F. Akter, Liuni Lipika Rozario, J. Akhter, Nighat Parveen, Poly Immaculata Costa, Sipra Mondal, Md. Obydul Hoq
Diabetes is a serious and growing global epidemic affecting people more in developing countries than developed countries, and is particularly devastating when triggered during pregnancy. Women with GDM are at increased risk of developing type 2 diabetes mellitus after pregnancy, while their offspring are prone to develop childhood obesity with type 2 diabetes in later life. This study was aimed to estimate the prevalence of gestational diabetes in Bangladeshi women. A descriptive cross-sectional study was carried out to estimate the prevalence of Gestational Diabetes among the pregnant women attending the OPD of BIRDEM Hospital. The study was conducted from January 2013 to April 2013. A non-probability purposive sampling technique was used. The data was collected using pre-tested self-administered semi-structured questionnaire and collected data was analyzed using SPSS 22.0 version. The finding of this study shows that, 42.3% of the respondents were in the age group 30-34 years and the mean age of the respondents was 24.4 (±4.94) years. The majority of the respondents (68.2%) were Muslim and it was also seen that 46.9% of the respondents had HSC level of education. More than half (65.9%) of the respondents were housewives and about 82.9% were from nuclear family. About 47.3% of the respondents had positive family history of hypertension and 34.4% had diabetes. More than half (57.7%) of the respondents did not maintain diabetic diet and 23.6% of the respondents had attended GDM program. The prevalence of gestational diabetes is 35.1%. There is need of appropriate intervention to control GDM and also risk indicator modifications in order to avoid complication regarding to mother and child. Asian Australas. J. Biosci. Biotechnol. 2019, 4 (2), 116-121
{"title":"Prevalence of gestational diabetes among the women attending the OPD of a selected tertiary level hospital in Bangladesh","authors":"F. Akter, Liuni Lipika Rozario, J. Akhter, Nighat Parveen, Poly Immaculata Costa, Sipra Mondal, Md. Obydul Hoq","doi":"10.3329/aajbb.v4i2.65071","DOIUrl":"https://doi.org/10.3329/aajbb.v4i2.65071","url":null,"abstract":"Diabetes is a serious and growing global epidemic affecting people more in developing countries than developed countries, and is particularly devastating when triggered during pregnancy. Women with GDM are at increased risk of developing type 2 diabetes mellitus after pregnancy, while their offspring are prone to develop childhood obesity with type 2 diabetes in later life. This study was aimed to estimate the prevalence of gestational diabetes in Bangladeshi women. A descriptive cross-sectional study was carried out to estimate the prevalence of Gestational Diabetes among the pregnant women attending the OPD of BIRDEM Hospital. The study was conducted from January 2013 to April 2013. A non-probability purposive sampling technique was used. The data was collected using pre-tested self-administered semi-structured questionnaire and collected data was analyzed using SPSS 22.0 version. The finding of this study shows that, 42.3% of the respondents were in the age group 30-34 years and the mean age of the respondents was 24.4 (±4.94) years. The majority of the respondents (68.2%) were Muslim and it was also seen that 46.9% of the respondents had HSC level of education. More than half (65.9%) of the respondents were housewives and about 82.9% were from nuclear family. About 47.3% of the respondents had positive family history of hypertension and 34.4% had diabetes. More than half (57.7%) of the respondents did not maintain diabetic diet and 23.6% of the respondents had attended GDM program. The prevalence of gestational diabetes is 35.1%. There is need of appropriate intervention to control GDM and also risk indicator modifications in order to avoid complication regarding to mother and child. \u0000Asian Australas. J. Biosci. Biotechnol. 2019, 4 (2), 116-121","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131685329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-31DOI: 10.3329/aajbb.v4i2.64953
Md. Abu Yousuf, M. Ershaduzzaman, Md. Alauddin, Tania Akhtar, M. Ali, Nupur Dhar, Md Taifur Rahman, M. Kabir, Md. Nuruzzaman Munsi, Md. Zakir Hassan, Md. Rafiqul Islam, M. Giasuddin
Peste des Petits Ruminants (PPR) is a highly fatal viral disease of goat and sheep. This research work was done in 2017-2018 by executing, surveillance and epidemiological studies to determine present status of circulating PPR virus and its molecular characterization in different areas of Bangladesh. cELISA was conducted to detect the PPR antibody and RT-PCR also used for identification of N gene PPRV. Sera samples and nasal swabs were collected from eight (8) selected villages under Meherpur sadar upazila of Meherpur district on questionnaire basis. Considering two villages as control and six villages as treatment villages. The total 1860 sera were collected at pre vaccination, 21 days, 3 months, 6 months of post vaccination at these selected areas that tested by cELISA and 8035 goat and sheep were received locally produced PPR Vaccine. Baseline study showed that a total of 950 household rear goats in selected 8 villages where number of goats per household ranges from 4.0-5.0. Deworming was done before vaccination in the treatment villages. Pre-vaccination status of six (6) treatment villages were 55.95%, 50.76%, 37.68%, 41.12%, 44.62% and 43.26% in Chakshamnagar, kola, Amjupi, Amdah, Gopalpur and Chadbill respectively, whereas in the control villages (2) seropositive were 40.00% and 42.57% in Doforpur and Mayamari, respectively. Overall 44.90% goats were seropositive against PPR Virus in treatment villages before vaccination. The Sera was analyzed from 21 days, 3 months and 6 months of post-vaccinated goat and sheep from the treatment (6) villages showed the average herd immunity level of goats and sheep rose to 89.10%, 93.25% and 93.37% respectively whereas in the control villages seropositive goats was 38.14%, 43.98% and 35.64% respectively. Awareness building campaigns with villagers have been conducted involving both men and women through the training, meeting, regular visit of household, distribution of poster and leaflet. The mortality and case fatality rate recorded were 7.4% and 18.8%, respectively due to PPR outbreaks. In clinical case, total 59 nasal swabs were molecular characterized by RT-PCR and 41 (69.49%) samples were N gene positive. Among them, the highest presence of PPR virus was recorded at Meherpur sadar upazila 80.77% (21 out of 26) samples was positive. The result of RT-PCR indicates the PPR virus circulating in the different regions of Bangladesh. It is reflected that locally produced PPR vaccine confers sufficient herd immunity that can protect PPR disease in goat and sheep which helps to meet global PPR control programme. Asian Australas. J. Biosci. Biotechnol. 2019, 4 (2), 88-96
{"title":"Sero-surveillance of circulating PPR virus and its molecular analysis in selected areas of Bangladesh","authors":"Md. Abu Yousuf, M. Ershaduzzaman, Md. Alauddin, Tania Akhtar, M. Ali, Nupur Dhar, Md Taifur Rahman, M. Kabir, Md. Nuruzzaman Munsi, Md. Zakir Hassan, Md. Rafiqul Islam, M. Giasuddin","doi":"10.3329/aajbb.v4i2.64953","DOIUrl":"https://doi.org/10.3329/aajbb.v4i2.64953","url":null,"abstract":"Peste des Petits Ruminants (PPR) is a highly fatal viral disease of goat and sheep. This research work was done in 2017-2018 by executing, surveillance and epidemiological studies to determine present status of circulating PPR virus and its molecular characterization in different areas of Bangladesh. cELISA was conducted to detect the PPR antibody and RT-PCR also used for identification of N gene PPRV. Sera samples and nasal swabs were collected from eight (8) selected villages under Meherpur sadar upazila of Meherpur district on questionnaire basis. Considering two villages as control and six villages as treatment villages. The total 1860 sera were collected at pre vaccination, 21 days, 3 months, 6 months of post vaccination at these selected areas that tested by cELISA and 8035 goat and sheep were received locally produced PPR Vaccine. Baseline study showed that a total of 950 household rear goats in selected 8 villages where number of goats per household ranges from 4.0-5.0. Deworming was done before vaccination in the treatment villages. Pre-vaccination status of six (6) treatment villages were 55.95%, 50.76%, 37.68%, 41.12%, 44.62% and 43.26% in Chakshamnagar, kola, Amjupi, Amdah, Gopalpur and Chadbill respectively, whereas in the control villages (2) seropositive were 40.00% and 42.57% in Doforpur and Mayamari, respectively. Overall 44.90% goats were seropositive against PPR Virus in treatment villages before vaccination. The Sera was analyzed from 21 days, 3 months and 6 months of post-vaccinated goat and sheep from the treatment (6) villages showed the average herd immunity level of goats and sheep rose to 89.10%, 93.25% and 93.37% respectively whereas in the control villages seropositive goats was 38.14%, 43.98% and 35.64% respectively. Awareness building campaigns with villagers have been conducted involving both men and women through the training, meeting, regular visit of household, distribution of poster and leaflet. The mortality and case fatality rate recorded were 7.4% and 18.8%, respectively due to PPR outbreaks. In clinical case, total 59 nasal swabs were molecular characterized by RT-PCR and 41 (69.49%) samples were N gene positive. Among them, the highest presence of PPR virus was recorded at Meherpur sadar upazila 80.77% (21 out of 26) samples was positive. The result of RT-PCR indicates the PPR virus circulating in the different regions of Bangladesh. It is reflected that locally produced PPR vaccine confers sufficient herd immunity that can protect PPR disease in goat and sheep which helps to meet global PPR control programme. \u0000Asian Australas. J. Biosci. Biotechnol. 2019, 4 (2), 88-96","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116963288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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