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Clinicopathological status of duck plague at Dinajpur district of Bangladesh 孟加拉国Dinajpur地区鸭鼠疫的临床病理状况
Pub Date : 2022-01-30 DOI: 10.3329/aajbb.v6i3.57717
Shabnam Mostari, SM Harun Ur Rashid, Md. Haydar Ali, M. Akther, Md. Nazrul Islam
Clinicopathological status of duck plague was investigated at different upazila of Dinajpur district during the period from October, 2017 to March, 2018. The clinical features emphasiging the mortality and prevalence, necropsy for gross morbid lesions, histopathological features were examined. The farm and flock history, managemental aspects, vaccination status, nutrition, etc. were recorded carefully. The data was collected and statistically analyzed. Farmer’s complaints about their affected birds were also considered and emphasized. The average mortality rate was recorded as 14.42% and prevalence was 19.81%. The sick birds clinically showed moderate to severe depression, ocular and nasal discharges, ataxia, dypsia. The affected organs were pathologically characterized as mild to moderate congestion and haemorrhages with misshapen, ruptured cystic ova and histopathologically characterized as mild to moderate architectural destruction, reactive cell infiltration.Asian Australas. J. Biosci. Biotechnol. 2021, 6 (3), 147-152
对2017年10月至2018年3月迪纳杰浦尔区不同区鸭鼠疫临床病理情况进行调查。检查了临床特征,强调死亡率和患病率,肉眼病变的尸检,组织病理学特征。详细记录猪场及畜群历史、管理情况、疫苗接种情况、营养状况等。收集数据并进行统计分析。农民对受感染禽鸟的抱怨也得到了考虑和强调。平均死亡率为14.42%,患病率为19.81%。病禽临床表现为中度至重度抑郁、眼鼻分泌物、共济失调、吞咽困难。受累器官病理表现为轻至中度充血出血伴畸形、囊性卵破裂,组织病理表现为轻至中度建筑破坏、反应性细胞浸润。亚洲Australas。j . Biosci。生物工程学报,2016,36 (3),357 - 357
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引用次数: 0
Landing and distribution of captured fish in selected landing centers in Kishoreganj haor, Bangladesh 在孟加拉国Kishoreganj haor选定的登陆中心登陆和分发捕获的鱼类
Pub Date : 2021-08-31 DOI: 10.3329/aajbb.v6i2.56143
A. Nowsad, M. Akter, A. Shahriar
The present study illustrates species diversity, landing quality and catch composition of fish in 10 fish landing centers of 5 haor upazilas of Kishoreganj haor for 12 months from January 2018 to December 2018. A total of 15 different types of fishing gears were detected to harvest fish in different stations throughout a year. Most of the fishing (46.88%) was conducted by small groups of 2 – 5 fishers, while 24.87% of the fishing was conducted by 6 – 10 fishers. The average depth of water in haor associated rivers and inundated lands during monsoon period varied from 4.87±0.99 to 15.2±6.71 and 2.6±0.55 to 6.2±2.68 feet respectively. Fifty different types of fish species were landed in the selected 10 landing centers, where both cultured fish and captured fish were present. Fishes under the Cyprinidae family included 9 species, viz. Labeo rohita. Gebelion catla, Cirrhinus cirrhosus, Labeo calbasu, Hypophthalmichthys nobilis, Hypophthalmichthys molitrix, Cyprinus carpio, Ctenopharyngodon idella, Labeo gonius covered a maximum of 12450.61±468.32 MT. The second largest landed fish under the family Schilbeidae included 4 species Silonia silondia, Mystus armatus, Mystus cavasius Pseudeutropius atherinoides. Other fish species landed were identified as: 4 species under the family Mastacembelidae, 2 species under Siluridae, 3 species under Clupidae, 1 species under Ambassidae, 2 species under Palaemonidae, 3 species under Channidae, 1 species under Gobidae, 2 species under Bagridae and 1 species from each of the family Heteropneustidae, Pangasiidae, Clariidae, Ailiidae, Botiidae, Nandidae, Cichlidae, Anabantidae, Osphronemidae, Ambassidae, Notopteridae, Notopteridae,Belonidae and Gobiidae. Highest number 14 species landed were under catfish category, followed by 9 species of major carp. The captured total fish landed in 10 landing centers in 12 month period accounted 12,574±1029.64 MT, but the cumulative total landed fish, including pond fish, was 15,795±1666061.93 MT, as calculated by 45 Aratdars of 10 landing centers. About 88.35% fishermen sold the captured fish at local arat, but the rest (11.65%) were sold to large traders (paiker), retailers and consumers. A 56.6% of the fishermen cleaned the captured fish by river water, while 43.4% fishermen did not use water for washing fish before selling. After harvest, 45.62% fishers used clean utensils for carrying fish to arat and 54.38% used uncleaned utensils. Average harvest per fishermen per day was 23.9 kg, of which 22.26 kg was sold in the market and 1.64 kg was used for own family consumption. Out of total fish landed, a 45.2% was transported by fish hold of the country boat, 21.9% by bamboo basket and 14.8% by plastic crate. Relatively small quantity of fish was transported by insulated ice box (4.7%), aluminum container (2.7%) and gunny bag (3.2%). A 32.6% fisherman used ice and 67.4 % fishermen did not use ice after harvesting of fish. During fishing season, fish and ice ratio used by the transpor
本研究于2018年1月至2018年12月,对基肖瑞甘省5个豪尔区10个鱼类上岸中心的物种多样性、上岸质量和渔获组成进行了研究。全年共检测到15种不同类型的渔具在不同的站点捕捞鱼类。大部分(46.88%)是由2 - 5人组成的小群体进行的,24.87%是由6 - 10人进行的。季风期河流和淹没地的平均水深分别为4.87±0.99 ~ 15.2±6.71英尺和2.6±0.55 ~ 6.2±2.68英尺。50种不同的鱼类在选定的10个登陆中心登陆,那里既有养殖鱼,也有捕获鱼。鲤科鱼类包括9种,即罗氏鲤科鱼类。鲇鱼(Gebelion catla)、Cirrhinus crighsus、Labeo calbasu、Hypophthalmichthys nobilis、Hypophthalmichthys molitrix、Cyprinus carpio、Ctenopharyngodon idella、Labeo gonius最大覆盖面积为12450.61±468.32 MT。鱼科第二大鱼类包括silondia Silonia、armatus Mystus、cavasius pseudoutropius atherinoides 4种。其他鱼类鉴定为:Mastacembelidae科4种、Siluridae科2种、Clupidae科3种、ambasidae科1种、Palaemonidae科2种、Channidae科3种、Gobidae科1种、Bagridae 2种、heteropnetitidae、Pangasiidae、Clariidae、Ailiidae、Botiidae、Nandidae、Cichlidae、Anabantidae、ospronemidae、Ambassidae、Notopteridae、Notopteridae、Belonidae、Gobiidae各1种。鲶鱼类最多,有14种,主要鲤鱼次之,有9种。10个着陆点的45台Aratdars计算,12个月期间10个着陆点的总捕获量为12,574±1029.64 MT,但累计着陆点总捕获量(包括塘鱼)为15,795±1666061.93 MT。约88.35%的渔民在当地渔场出售捕获的鱼,其余(11.65%)则出售给大型贸易商(捕鱼者)、零售商和消费者。56.6%的渔民用河水清洗捕获的鱼,而43.4%的渔民在出售前没有用水清洗鱼。捕捞后,45.62%的渔民使用干净的工具运鱼,54.38%的渔民使用未清洁的工具运鱼。每个渔民每天的平均收获量为23.9公斤,其中22.26公斤在市场上出售,1.64公斤供自己家庭消费。在所有上岸的鱼中,45.2%是由乡村船的鱼舱运输,21.9%是用竹篮运输,14.8%是用塑料板条箱运输。采用保温冷藏箱(4.7%)、铝制集装箱(2.7%)和麻袋运输的鱼类数量较少(3.2%)。32.6%的渔民在捕渔后使用冰,67.4%的渔民在捕渔后不使用冰。在捕捞季节,运输船使用的鱼冰比为2.62:1。Karimganj的Chamra Ghat的拍卖商数量最多,Nikli的拍卖商数量最少,每个拍卖商用于鱼类业务的面积分别为55±1.44和25±8.42。亚洲Australas。j . Biosci。生物工程学报,2016,36 (2):775 - 788
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引用次数: 0
Aqua medicines, drugs and chemicals (AMDC) used in freshwater aquaculture of South-Eastern Bangladesh 孟加拉国东南部淡水水产养殖中使用的水药品、药物和化学品
Pub Date : 2021-08-31 DOI: 10.3329/aajbb.v6i2.56145
A. Hossain, S. Islam, Abdulla- Al-Asif, H. Rahman
A broad variety of aquaculture-related medications, drugs, and chemicals (AMDC) are extensively used in the aquaculture industry in South-Eastern Bangladesh. Fish farmers are worried about the quality of their final product, and disease outbreaks must be stopped at all costs. Farmers are sometimes one ahead of the curve when it comes to producing healthy final products by including probiotics, vitamins, and minerals into their aquaculture setups to promote early and disease-free output. However, the current study was carried out in south eastern Bangladesh, specifically in the highly dense aquaculture regions of Chandpur, Cumilla, and Feni district (17 upazilas), from November 2016 to January 2018. Questions were asked through interviews and a Focus Group Discussion (FGD) was held to gather primary data. The major target groups were aquaculture farmers, AMDC shops, pharmaceutical company employees, and hatchery owners. In this three-county area, according to the findings from the thorough research, there are a total of 33 companies that advertise 330 generics brand products via their own distributional channels. Among the available AMDC products in the study area, growth promoters were mostly abundant products among all categories while other products such as predator removal products, insecticides and ectoparasiticides, water quality and pond management, plankton producer, plankton bloom cleaner, disinfectant and disease treatment, toxic gas reducer, pH controller, oxygen supply, stress reducer, growth promoter, probiotics and antibiotics were most selling products to the farmers. The present study revealed 19 generic of antibiotics were available and prescribed by the AMDC vendors or aquaculture disease consultants (ADC) around the regions. Additionally, the research also included the dosages of AMDC and the method of administration in the aquaculture pond, which will assist both the farmers and the ADC in selecting and suggesting the appropriate medications or treatments that may be beneficial to the farmers in the long run.Asian Australas. J. Biosci. Biotechnol. 2021, 6 (2), 103-127
孟加拉国东南部的水产养殖业广泛使用各种与水产养殖有关的药物、药品和化学品(AMDC)。养鱼户担心最终产品的质量,必须不惜一切代价阻止疾病爆发。在生产健康的最终产品时,农民有时会走在前面,在他们的水产养殖设施中加入益生菌、维生素和矿物质,以促进早期和无病产量。然而,目前的研究是在2016年11月至2018年1月期间在孟加拉国东南部进行的,特别是在Chandpur、Cumilla和Feni区(17个upazilas)的高密度水产养殖区。通过访谈提出了问题,并举行了焦点小组讨论(FGD)以收集原始数据。主要目标群体是水产养殖户、AMDC商店、制药公司员工和孵化场所有者。在这三县地区,根据深入研究的结果,共有33家公司通过自己的分销渠道宣传330种仿制药品牌产品。在研究区可获得的AMDC产品中,生长促进剂是各品类中最丰富的产品,而捕食者清除产品、杀虫剂和除虫剂、水质和池塘管理、浮游生物产生剂、浮游生物水华清洁剂、消毒剂和疾病治疗、有毒气体减少剂、pH控制剂、供氧、减压剂、生长促进剂、益生菌和抗生素是向养殖户销售最多的产品。本研究发现,这些地区的AMDC供应商或水产养殖疾病顾问(ADC)可获得19种非专利抗生素。此外,该研究还包括水产养殖池中AMDC的剂量和给药方法,这将有助于农民和ADC选择和建议适当的药物或治疗方法,从长远来看可能对农民有益。亚洲Australas。j . Biosci。生物工程学报,2016,36 (2):1093 - 1097
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引用次数: 2
Estimation of residue degradation of cypermethrin and chlorpyrifos in brinjal, tomato and cauliflower under supervised field trial 氯氰菊酯和毒死蜱在茄子、番茄和菜花中的残留降解评价
Pub Date : 2021-08-31 DOI: 10.3329/aajbb.v6i2.56141
M. Ahmed, Mohammad Dalower Hossain Prodhan, A. Begum, M. Afroze, D. Sarker
A study was carried out to detect and estimate the residue of cypermethrin and chlorpyrifos in brinjal, tomato and cauliflower using Gas Chromatography (GC) coupled with Electron Capture Detector (ECD) and Flame Thermionic Detector (FTD). Three supervised field trials were conducted and sprayed with the recommended dose of cypermethrin (1 ml/L of water) and chlorpyrifos (3 ml/L of water) in three vegetables by knapsack sprayer. Samples were collected at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11 days after spray (DAS). The quantity of cypermethrin residue was above EU-MRLs (Maximum Residue Limit set by European Union) up to 4 DAS with 0.538 mg/kg in cauliflower; 3 DAS with 0.508 mg/kg in brinjal and 0.695 mg/kg in tomato. The quantity decreased down 0.328-0.019 mg/kg in cauliflower (4-9 DAS); 0.098-0.012 mg/kg (4-7 DAS) in brinjal and 0.458-0.022 mg/kg (4-7 DAS) in tomato which were below EU-MRLs (0.5 mg/kg). The quantity of chlorpyrifos residue was above EU-MRLs up to 9 DAS with 0.012 mg/kg in cauliflower but in brinjal and tomato, the detected quantities were above EU-MRLs up to 7 DAS with 0.029 mg/kg and 0.017 mg/kg residue. No residue was detected from brinjal and tomato samples collected at 8 DAS. But in cauliflower, no residue was detected at 10 days after spray. So, brinjal, tomato and cauliflower can be harvested safely at 4 DAS and 5 DAS for cypermethrin and in case of chlorpyrifos it was 8 DAS in brinjal and tomato and 10 DAS in cauliflower.Asian Australas. J. Biosci. Biotechnol. 2021, 6 (2), 60-67
采用气相色谱联用电子捕获检测器(ECD)和火焰热离子检测器(FTD)对茄子、番茄和菜花中氯氰菊酯和毒死蜱的残留量进行了检测和评价。进行了3次监督田间试验,采用背负式喷雾器对3种蔬菜分别喷洒推荐剂量的氯氰菊酯(1ml /L水)和毒死蜱(3ml /L水)。分别于喷雾后0、1、2、3、4、5、6、7、8、9、10和11天采集样品。菜花中氯氰菊酯残留量高于欧盟最大残留限量(mrls)达4 DAS,为0.538 mg/kg;3 DAS,茄子中含量为0.508 mg/kg,番茄中含量为0.695 mg/kg。菜花(4-9 DAS)中含量下降0.328 ~ 0.019 mg/kg;茄子为0.098 ~ 0.012 mg/kg (4-7 DAS),番茄为0.458 ~ 0.022 mg/kg (4-7 DAS),均低于欧盟最大限量(0.5 mg/kg)。菜花中毒死蜱残留量为0.012 mg/kg,高于欧盟最大残留限量9 DAS;茄子和番茄中毒死蜱残留量为0.029 mg/kg,高于欧盟最大残留限量7 DAS,高于欧盟最大残留限量0.017 mg/kg。8 DAS采集的茄子和番茄样品未检出残留。而在花椰菜中,喷洒10天后没有残留。因此,茄子、番茄和花椰菜在氯氰菊酯浓度为4 DAS和5 DAS的情况下可以安全收获,而毒死蜱在茄子和番茄中的浓度为8 DAS,在花椰菜中的浓度为10 DAS。亚洲Australas。j . Biosci。生物工程学报,2017,36 (2):60-67
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引用次数: 6
Effect of hydrogen peroxide (H2O2) on shelf life and bacterial population of raw milk 过氧化氢(H2O2)对原料奶保质期和细菌数量的影响
Pub Date : 2021-07-18 DOI: 10.3329/AAJBB.V6I1.54877
Jowel Rana, Md Anwar Hossain, Dipok Kumar Sarkar, Md Ashraful Alom, S. Sarkar, Md. Hafizur Rahman, M. A. Islam, M. Islam
The present study was conducted to assess the feasibility of hydrogen peroxide (H2O2) for milk preservation and also to investigate the effects of H2O2 on growth of bacteria population in milk. Milk samples were collected from Bangladesh Agricultural University dairy farm and preserved with 0.12% H2O2 of 10% concentration on the basis of volume of milk and milk without H2O2 addition treated as control sample. Parameters used to monitor the shelf life of milk were organoleptic and chemical tests. All milk samples were kept in glass container at room temperature (28-31°C) in the laboratory. Organoleptic parameters (color, flavor, texture, taste) and chemical tests (acidity %, COB test and pH) were done every one hour interval until spoilage. Standard Plate Count (SPC) of the samples was done two times, initially and after spoilage of milk. Acidity% increased gradually in all sample but this increase was rapid in control sample than H2O2 treated sample. From COB test it was found that raw milk sample gave positive result at 10th hour but 0.12% H2O2 treated sample (after 20 min of milking ) and 0.12% H2O2 treated sample (after 3 hrs. of milking ) gave positive result 14 and 15th hour respectively. From the result it was observed that shelf life of milk with H2O2 increased. From the SPC result it was found that the bacterial growth rate is very high in control milk sample compare to H2O2 treated milk sample. Significant difference (p<0.01 was found in case of bacterial growth rate of the milk samples. The results of this experiment indicated that H2O2 be used as an effective preservative for prolonging milk preservation. So H2O2 can be used as an effective milk preservative for prolonging milk storage in environmental condition. Asian Australas. J. Biosci. Biotechnol. 2021, 6 (1), 19-25
本研究旨在评估过氧化氢(H2O2)用于牛奶保鲜的可行性,并探讨H2O2对牛奶中细菌种群生长的影响。牛奶样品采集自孟加拉国农业大学奶牛场,以牛奶体积为基础,用浓度为10%的0.12% H2O2保存,不添加H2O2的牛奶作为对照样品。用于监测牛奶保质期的参数是感官测试和化学测试。所有牛奶样品均保存在实验室室温(28-31℃)的玻璃容器中。感官参数(颜色、风味、质地、口感)和化学测试(酸度%、COB测试和pH值)每隔一小时进行一次,直到变质。样品的标准板计数(SPC)做了两次,最初和牛奶变质后。所有样品的酸度都是逐渐增加的,但对照样品的酸度比H2O2处理后的样品增加得快。COB测试发现原料奶样品在第10小时呈阳性,但0.12% H2O2处理样品(挤奶20分钟后)和0.12% H2O2处理样品(挤奶3小时后)呈阳性。第14和15小时分别为阳性。结果表明,添加H2O2后,牛奶的保质期延长。从SPC结果中发现,与H2O2处理的牛奶样品相比,对照牛奶样品中的细菌生长速度非常高。乳样细菌生长速率差异有统计学意义(p<0.01)。实验结果表明,H2O2可以作为一种有效的保鲜剂来延长牛奶的保鲜期。因此,H2O2可以作为一种有效的牛奶防腐剂,在环境条件下延长牛奶的储存时间。亚洲Australas。j . Biosci。生物工程学报,2016,36 (1),19-25
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引用次数: 2
Comparing the performances of multiple rainfall-runoff models of a karst watershed 喀斯特流域多种降雨径流模型的性能比较
Pub Date : 2021-07-18 DOI: 10.3329/AAJBB.V6I1.54878
M. Hasan, Shariot Ullah, A. Saha, M. M. Amin
Different modeling concepts, a simple (black-box) to a fully distributed modeling (white-box), were used to develop a rainfall-runoff model based on the watershed characteristics to estimate runoff at the watershed outlet. A conceptual (grey-box) model is usually a balance between the black-box and white-box model. In this study, three grey-box models were developed by varying model structures for a karst watershed. The performance of the grey-box models was evaluated and compared with a semi-distributed type (white-box) model that was developed using the Soil and Water Assessment Tool in a previous study. The evaluation was carried out using goodness-of-fit statistics and extreme flow analysis using WETSPRO (Water Engineering Time Series Processing tool). Nash-Sutcliffe efficiencies (NSE) of the grey-box models were from 0.39 to 0.77 in the calibration period and from 0.30 to 0.61 in the validation period. However, the white-box model performed better in terms of NSE but has a higher bias. The best grey-box model performed better in simulating extreme flow, whereas the white-box (SWAT) model adequately simulated daily flows. Asian Australas. J. Biosci. Biotechnol. 2021, 6 (1), 26-39
利用简单(黑盒)和完全分布式(白盒)模型的不同建模概念,建立了基于流域特征的降雨-径流模型,用于估算流域出水口的径流量。概念性(灰盒)模型通常是黑盒和白盒模型之间的平衡。本文通过不同的模型结构,建立了喀斯特流域的三个灰盒模型。对灰盒模型的性能进行了评估,并与先前研究中使用水土评估工具开发的半分布式(白盒)模型进行了比较。评估采用拟合优度统计和极限流量分析,使用WETSPRO(水工程时间序列处理工具)。灰盒模型的Nash-Sutcliffe效率(NSE)在校正期为0.39 ~ 0.77,在验证期为0.30 ~ 0.61。然而,白盒模型在NSE方面表现更好,但具有更高的偏差。最佳的灰盒模型能较好地模拟极端流量,而白盒(SWAT)模型能较好地模拟日流量。亚洲Australas。j . Biosci。生物工程学报,2016,36 (1),26-39
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引用次数: 0
Tradition of raw milk consumption and its health impact among people living in the coastal regions of Bangladesh 孟加拉国沿海地区居民的生奶消费传统及其对健康的影响
Pub Date : 2021-07-18 DOI: 10.3329/AAJBB.V6I1.54876
SM Tanvir Reza, W. Ansari, Robiul Karim, A. Anower, F. I. Rume
Milk is a nutrient-rich liquid food for human and animals. However, owing to its complex biochemical structure and high water activity, it serves as an outstanding microorganism growth medium under suitable conditions. People in Bangladesh's coastal areas have a lifestyle and tradition that includes drinking raw milk for nutrition or as an energy drink, which presents a health risk to them. The present cross-sectional study was carried out to determine the reasons for drinking raw milk in coastal areas, as well as the public health impact of doing so. A total of 100 respondents were interviewed and subsequently, milk samples were collected for quality testing through methylene blue reduction test (MBRT). Among 100 respondent 70 were consumers and 30 were farmers. Results showed that the samples of farmers were better than that of consumers. The majority of raw milk consumers were male who thought raw milk was more nutritious than heat-treated milk. Furthermore, the majority of them were professionally engaged in farming. Both consumers and farmers had less educational qualifications, managed their cattle in dirty environments, practiced extensive grazing system on communal grazing areas and around one fourth of them vaccinated their animals. Furthermore, the findings revealed that 40% of respondents were between the ages of 35 and 45, with 62% reporting no health complications as a result of drinking raw milk and the remainder reporting mainly gastrointestinal problems. According to the findings of this report, the quality of raw cow milk was poor; unhygienic practices and poor animal husbandry at farm level predisposed farmers, consumers and the public to risk of contracting milk-borne infections and associated bacterial resistances. It is recommended that veterinarians, extension officers and all stakeholders should play their roles in ensuring safe quality milk supply to consumers. Asian Australas. J. Biosci. Biotechnol. 2021, 6 (1), 10-18
牛奶是人类和动物的营养丰富的流质食物。然而,由于其复杂的生化结构和较高的水活性,在合适的条件下,它是一种优秀的微生物生长培养基。孟加拉国沿海地区的人们有一种生活方式和传统,包括饮用生牛奶作为营养或能量饮料,这对他们的健康构成了威胁。目前进行的横断面研究是为了确定沿海地区饮用生牛奶的原因,以及这样做对公共卫生的影响。对100名调查对象进行访谈,采集牛奶样品,通过亚甲基蓝还原试验(MBRT)进行质量检测。在100名受访者中,70名是消费者,30名是农民。结果表明,农民样品优于消费者样品。大多数生奶消费者是男性,他们认为生奶比热处理过的牛奶更有营养。此外,他们中的大多数是专业从事农业。消费者和农民的教育程度都较低,在肮脏的环境中管理他们的牛,在公共牧区实行广泛的放牧制度,大约四分之一的人给他们的牲畜接种疫苗。此外,调查结果显示,40%的受访者年龄在35岁至45岁之间,62%的受访者表示没有因饮用生牛奶而出现健康并发症,其余的人主要报告肠胃问题。根据这份报告的调查结果,生牛奶的质量很差;农场一级不卫生的做法和糟糕的畜牧业使农民、消费者和公众容易受到乳源性感染和相关细菌耐药性的威胁。建议兽医、推广人员和所有利益相关者在确保向消费者提供安全优质的牛奶方面发挥作用。亚洲Australas。j . Biosci。生物工程学报,2017,6 (1),10-18
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引用次数: 0
Prevalence and antimicrobial susceptibility patterns of Vibrio cholerae in dairy excreta 奶牛排泄物中霍乱弧菌的流行及药敏模式
Pub Date : 2021-07-18 DOI: 10.3329/AAJBB.V6I1.54879
Nilofa Eashmen, M. Arif, B. Sarker, M. R. Akter, S. Kabir
Vibrio cholerae is a major etiological agent of human diarrhoea and has become epidemic across the world in the recent past. A cross-sectional study was conducted to assess the prevalence of V. cholerae from dairy excreta along with antimicrobial resistant status of the isolates. A total 50 samples were collected from 50 different household manure pit located at Bangladesh Agriculture University (BAU) surrounding area, Mymensingh. Alkaline peptone water was used for enrichment of the samples followed by inoculation onto thiosulfate citrate bile salt sucrose (TCBS) agar media for the isolation of Vibrio spp., which were further confirmed via Vibrio genus specific molecular assay. Biochemical tests were performed to identify V. cholerae from the isolates of Vibrio spp. Out of 50 samples 17 (34%) were confirmed as Vibrio spp. as they produced characteristic yellow colonies on TCBS agar and had found to possess recombinase A gene that confirmed the identity of Vibrio spp. From this 17 Vibrio isolates, 6 (12% in total from 50 samples) were identified as V. cholerae based on different biochemical tests. All the isolates fermented glucose, maltose, sucrose and mannitol with the production of only acid. The isolates were positive in oxidase, gelatinase, methyl-red (MR) and indole test, but negative in case of voges-proskaure (VP) test. In antimicrobial susceptibility testing, V. cholerae isolates showed 100% sensitivity to gentamycin, chloramphenicol and tetracycline with moderate sensitivity to ciprofloxacin and co-trimoxazole. A high level of resistance was observed to ampicillin (100%) followed by moderate resistance to erythromycin and imipenem. In the present study about 33.33% (n = 2) of 6 isolated V. cholerae were found to be multidrug resistant (MDR) as they demonstrated resistant against 3 antimicrobial agents. The findings of this study substantiate the presence of MDR V. cholerae in the dairy excreta, which indicates the role of domestic animals to serve as a reservoir that might pose a health risk to human. Hygienic management of animal waste is needed to reduce the burden of human illness. Asian Australas. J. Biosci. Biotechnol. 2021, 6 (1), 40-49
霍乱弧菌是人类腹泻的主要病原,近年来在世界各地流行。进行了一项横断面研究,以评估奶牛排泄物中霍乱弧菌的流行情况以及分离株的抗微生物药物耐药性状况。从位于孟加拉国农业大学(BAU)迈门辛格周边地区的50个不同的家庭粪坑共收集了50个样本。用碱性蛋白胨水对样品进行富集,然后接种于柠檬酸硫代硫酸盐胆汁盐蔗糖(TCBS)琼脂培养基上分离出弧菌,并通过弧菌属特异性分子测定进一步证实。通过生化试验鉴定霍乱弧菌,50份样品中有17份(34%)为霍乱弧菌,因为它们在TCBS琼脂上产生特有的黄色菌落,并且发现含有重组酶A基因,证实了弧菌的身份。所有分离菌均发酵葡萄糖、麦芽糖、蔗糖和甘露醇,仅产酸。菌株氧化酶、明胶酶、甲基红(MR)和吲哚试验均呈阳性,voges-proskaure (VP)试验呈阴性。在药敏试验中,霍乱弧菌对庆大霉素、氯霉素和四环素的敏感性为100%,对环丙沙星和复方新诺明的敏感性中等。对氨苄西林高度耐药(100%),其次为对红霉素和亚胺培南的中度耐药。在本研究中,6株分离霍乱弧菌中约33.33% (n = 2)被发现对3种抗菌素具有耐多药(MDR)耐药性。本研究的结果证实了奶牛排泄物中存在耐多药霍乱弧菌,这表明家畜可能是对人类健康构成威胁的宿主。需要对动物粪便进行卫生管理,以减轻人类疾病的负担。亚洲Australas。j . Biosci。生物工程学报,2017,36 (1),40-49
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引用次数: 0
Effects of different dietary energy and protein levels on the performance and carcass characteristics of native hilly chicken during growing phase in confinement 禁闭期不同饲粮能量和蛋白质水平对土鸡生长性能和胴体特性的影响
Pub Date : 2021-07-18 DOI: 10.3329/AAJBB.V6I1.54875
H. Khatun, Shakila Faruqe, G. Mostafa
Two hundred and sixteen 10 weeks of age hilly chickens were used to determine the effects of dietary energy and protein level on growth performance, carcass characteristics and meat quality. The chicks were randomly allotted in to 3×3 factorial in a completely randomize design. Three levels of energy (2600, 2700 and 2800 ME kcal/kg) and three levels of dietary proteins (16, 17 and 18% CP) were offered ad libitum to the chicks from 10-16 weeks of age. There were no significant interaction effect between dietary protein and energy levels in the diets. At 10-16 weeks of age hilly chickens fed with the medium protein diet (17% CP) showed shortened feed intake (p<0.001) but FCR found better in 16% CP diet. Dietary protein levels higher than 16% CP did not show any significant effect on growth performance. However hilly chicken fed with lower protein diet converted protein to body weight and body weight gain more efficiently than those fed higher protein diets. Dietary energy contents of 2600, 2700 and 2800 ME kcal/kg did not affect the growth performance of hilly chicken except ME, CP intake and protein conversion ratio. ME and CP intake was increased with increasing dietary ME and CP levels (p<0.000). Protein utilization was better (p<0.05) in higher (2800 ME kcal/kg) and medium (2700 ME kcal/kg) ME level diet. L*, a* and b* of breast meat was not affected by dietary ME and CP (p>0.05). Dietary energy and protein level did not significantly (p>0.05) affect the drip loss, cooking loss and pH of breast meat between the treatments. Based on the data of growth performance, carcass characteristics and meat quality, the optimal dietary ME requirement of hilly chicken from 10-16 weeks of age is 2800 ME kcal/kg and the CP requirement is 16%. Asian Australas. J. Biosci. Biotechnol. 2021, 6 (1), 1-9
以216只10周龄丘陵鸡为试验对象,研究饲粮能量和蛋白质水平对生长性能、胴体特性和肉品质的影响。在完全随机设计中,小鸡被随机分配到3×3阶乘中。10-16周龄雏鸡可随意添加3个能量水平(2600、2700和2800 ME kcal/kg)和3个蛋白质水平(16、17和18% CP)。饲粮中蛋白质和能量水平之间无显著交互作用。10 ~ 16周龄时,中等蛋白质饲粮(17% CP)的采食量显著缩短(p0.05)。饲粮能量和蛋白质水平对胸肉滴漏损失、蒸煮损失和pH值无显著影响(p>0.05)。根据生长性能、胴体特性和肉品质等指标,10 ~ 16周龄丘陵鸡饲粮代谢能适宜需水量为2800 mekcal /kg,粗蛋白质适宜需水量为16%。亚洲Australas。j . Biosci。生物工程学报,2017,6 (1):1-9
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引用次数: 2
Status of drying fish, marketing channel and associated problems in the Kuakata coast of Patuakhali district, Bangladesh 孟加拉国Patuakhali地区Kuakata海岸干鱼现状、销售渠道及相关问题
Pub Date : 2020-08-31 DOI: 10.3329/AAJBB.V5I2.53863
Jabir Al Mehedi, A. Shahriar, S. M. Fahim, Giass Uddin
The present study was conducted to know the fish drying activities, species used in fish drying and marketing channel of dried fish in Kuakata coast of Patuakhali district of Bangladesh. Sea fishes are mostly used for drying and dry fish producers collected raw fishes from arat (landing centers) (70%), fishermen (25%) and little amount from own capture (5%). It was found that 21 fish species were used for drying among them 3 species (Harpadon nehereus, Setipinna phasa, Lepturacanthus savala) were commonly used and other 18 species were fairly used. The peak fish drying season was observed at mid-October to mid-February (in Bengali Kartik to Magh). It was observed that the dry fish producers used salt to produce good quality dry fish and salt mixing rate is 2 kg salt for 30-35 kg raw fish. The duration of drying at normal weather condition is varied between 3-5 days depending on the size of fishes. After drying, the dried fishes were packed in either jute bags or polythene bags and stored at room temperature for marketing. About 95% dried fish products are sold in the wholesale market at Chittagong, Syedpur, and Dhaka and rest 5% sold to the local retail market. However, three (3) step dried fish marketing channels were observed in the study area. The producers sold their product at very lower price and it ranged between Tk. 120-3200 per Kg. The retail price of the dried fishes ranged from Tk. 200-3500 per Kg. The dry fish producers and intermediaries faced various problems like inadequate capital, natural calamities, lack of scientific knowledge and technology, price instability, lack of transport facilities, inadequate storage facilities, lack of physical marketing facilities and marketing information. Asian Australas. J. Biosci. Biotechnol. 2020, 5 (2), 48-59
本研究旨在了解孟加拉国帕图阿卡里区Kuakata海岸的鱼干活动、鱼干所用种类和鱼干销售渠道。海鱼主要用于干燥,干鱼生产者从arat(着陆中心)收集生鱼(70%),渔民(25%)和少量自己捕获(5%)。结果表明,用于干燥的鱼类有21种,其中常用的有3种(Harpadon nehereus, Setipinna phasa, Lepturacanthus savala),其他18种被合理利用。10月中旬至2月中旬(孟加拉Kartik至Magh)为鱼类干季高峰。经观察,干鱼生产者使用盐生产出优质的干鱼,30-35公斤生鱼的盐混合率为2公斤盐。在正常的天气条件下,干燥的时间根据鱼的大小在3-5天之间变化。晾干后,将干鱼装在黄麻袋或聚乙烯袋中,在室温下储存,以待销售。约95%的鱼干产品在吉大港、西布尔和达卡的批发市场销售,其余5%销往当地零售市场。然而,在研究区域观察到三(3)步鱼干销售渠道。生产商以非常低的价格出售他们的产品,价格在每公斤120-3200塔卡之间。干鱼的零售价格从每公斤200-3500塔卡不等。干鱼生产者和中间商面临着资金不足、自然灾害、缺乏科学知识和技术、价格不稳定、缺乏运输设施、缺乏储存设施、缺乏实物销售设施和营销信息等各种问题。亚洲Australas。j . Biosci。生物技术,2020,5 (2),48-59
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引用次数: 3
期刊
Asian-Australasian Journal of Bioscience and Biotechnology
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