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Evaluation of pharmacognostic and phytochemical profile of Spigelia anthelmia linn leaves 刺棘叶的生药学和植物化学特征评价
IF 0.3 Q4 PLANT SCIENCES Pub Date : 2019-01-01 DOI: 10.5281/zenodo.3518849
O. L. Awotedu, Paul Oluwatimilehin Ogunbamowo
The medicinal credence of Spigelia anthelmia suggests the plant as an important herbal remedy in the treatment of asthma and helminthiasis. A valid pharmacognostic assessment is done to give a standard and quality identification of the medicinal plant. A fresh leaf and a powdered form were evaluated. The foliar micro-morphology (qualitative and quantitative), microscopy, chemo-microscopy, and phytochemical composition were assessed using standard methods. The result indicates that for micromorphology, the cells are rectangular to polygonal with very large cells for both abaxial and adaxial epidermis. It has a straight anticlinal wall. stomata type is anomocytic for both the epidermis which means the leaf is amphistomatic. Trichomes present on the abaxial and adaxial are uniseriate and non-glandular, while they are absent on the adaxial epidermis. The stomata index for abaxial is (10.42%), while for adaxial is (5.88%). The cell length for abaxial (84.55 μm) and adaxial is (81.92 μm), while the cell width for abaxial is (58.15 μm) and for adaxial is (57.85 μm). The cell density is higher on the abaxial with (43.0 μm) and lesser on the adaxial with (32.0 μm). Mean Stomata number on the lower epidermis is (5), while on the adaxial is (2). The macroscopic features identified shows that the leaves are opposite, simple and entire, having a cylindrical shape with horny texture, the bark is coarse and the surface is smooth with a spot of brown nodules. The trunk is simple, without thorns but hairy. The leaf epidermis is straight with numerous starch grains and the crystals of calcium oxalate are present on the abaxial epidermis and absent on the adaxial. It has a granular fracture surface; its color is greyish green with a faint characteristic odor. The chemo-microscopic analysis shows that Starch, calcium carbonate crystals and cellulose are present while lignin, fats, calcium oxalate crystals, and mucilage were absent. The phytochemical screening shows that flavonoid, alkaloid, saponin, phenols, and tannin are present except, cardiac glycoside and anthraquinone. The foliar micromorphology, the macroscopy and phytochemical composition of S. anthelmia have provided proper information for its identification and authentication which can enable it to be included in the official pharmacopeia of Nigerian medicinal plants.
药用证据表明,该植物是治疗哮喘和寄生虫病的重要中药。对该药材进行了有效的生药学评价,为该药材的质量鉴定提供了标准。评估了新鲜叶子和粉末形式。采用标准方法对叶片微观形态(定性和定量)、显微镜、化学显微镜和植物化学成分进行了评估。结果表明,在微形态上,细胞呈矩形至多边形,在背面和正面表皮上细胞都非常大。它有一个直的背斜壁。两层表皮的气孔类型都是无形细胞,这意味着叶片是两形气孔。毛状体存在于背面和正面,单列和非腺状,而在正面表皮上不存在。气孔指数为(10.42%),正面气孔指数为(5.88%)。胞长为84.55 μm,近轴为81.92 μm,宽为58.15 μm,近轴为57.85 μm。细胞密度在(43.0 μm)轴向上较大,在(32.0 μm)轴向上较小。下表皮平均气孔数为(5),正面平均气孔数为(2)。经宏观特征鉴定,叶片对生,单生全叶,呈圆柱形,具角质质地,树皮粗糙,表面光滑,有褐色结节斑。树干很简单,没有刺,但有毛。叶表皮直,淀粉粒多,背面有草酸钙结晶,正面无。断口呈颗粒状;其颜色为灰绿色,带有微弱的特征气味。化学显微镜分析表明,淀粉、碳酸钙晶体和纤维素存在,而木质素、脂肪、草酸钙晶体和粘液不存在。植物化学筛选表明,除心糖苷和蒽醌外,还含有黄酮类、生物碱、皂苷、酚类和单宁。该植物的叶面显微形态、宏观结构和植物化学成分为其鉴定和鉴定提供了依据,可列入尼日利亚药用植物官方药典。
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引用次数: 1
Cytosine methylation polymorphisms in cotton using TD-MS-RAPD-PCR 利用TD-MS-RAPD-PCR分析棉花胞嘧啶甲基化多态性
IF 0.3 Q4 PLANT SCIENCES Pub Date : 2019-01-01 DOI: 10.5281/ZENODO.3518790
M. Karaca, A. Aydin, Ayse Gul Ince
Methylation in DNA and chemical modification in histone proteins are the two most studied epigenetic mechanisms in plants. There exist low-throughput and high-throughput DNA methylation detection techniques in epigenetic research. In this study, touch-down polymerase chain reactions methylation sensitive-random amplified polymorphic DNA (TD-MSRAPD) technique was used to investigate cytosine methylation differences among three cotton varieties; Texas Marker 1 (TM-1), Pima 3-79 (3-79) and Maydos Yerlisi (MY), belonging to Gossypium hirsutum L., G. barbadense L., and G. herbaceum L., respectively. Genomic DNA samples extracted from the mature seeds of these varieties were treated with MspI, a relative methylation-insensitive restriction enzyme and HpaII, a methylation-sensitive restriction enzyme before touch-down polymerase chain reactions. Among 16 oligonucleotide primers used, three primers (AT03, W15, and C08) resulted in methylation polymorphisms among three varieties. TD-MS-RAPD-PCR method was cost-effective, required a simple method and basic instrumentation, and could easily be performed in our laboratory with basic setup using a regular DNA thermal cycler and DNA gel electrophoresis system, however, the level of methylation polymorphisms detected with this method were very low in cotton. We concluded that the low level of polymorphisms among the three cotton species were probably due to low occurrences of CCGG sites within the cotton genome. We also noted that TDMS- RAPD-PCR method could be used in primary scanning studies in epigenetic research.
DNA的甲基化和组蛋白的化学修饰是植物中研究最多的两种表观遗传机制。表观遗传学研究中存在低通量和高通量DNA甲基化检测技术。本研究采用触地聚合酶链反应甲基化敏感-随机扩增多态性DNA (TD-MSRAPD)技术研究了3个棉花品种胞嘧啶甲基化差异;德克萨斯标记1号(TM-1)、皮马3-79(3-79)和Maydos Yerlisi (MY),分别属于棉属、巴氏棉属和草属。从这些品种的成熟种子中提取的基因组DNA样本在接触聚合酶链反应之前用相对甲基化不敏感的限制性内切酶MspI和甲基化敏感的限制性内切酶HpaII处理。在16条引物中,有3条引物(AT03、W15和C08)在3个品种间存在甲基化多态性。TD-MS-RAPD-PCR方法成本低,方法简单,仪器简单,在我们的实验室中使用常规的DNA热循环仪和DNA凝胶电泳系统即可进行基本设置,但该方法在棉花中检测到的甲基化多态性水平很低。我们认为,3种棉花的低多态性可能是由于CCGG位点在棉花基因组中的发生率较低。我们也注意到TDMS- RAPD-PCR方法可用于表观遗传学研究的初级扫描研究。
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引用次数: 6
Infraspecific morphological and molecular variation of Linum nervosum (Linaceae) in Iran 伊朗神经Linum(Linaceae)的亚特异性形态和分子变异
IF 0.3 Q4 PLANT SCIENCES Pub Date : 2018-05-26 DOI: 10.5281/ZENODO.1253614
Maryam Rashnou-Taei, M. Sheidai, S. Talebi, Z. Noormohammadi, F. Koohdar
Linum nervosum is among species that can hybridize with L. usitatissimum and produce fertile offsprings. Genetic diversity analysis of this wild relative of flax is important from conservation and breeding points of view. In the present study, 55 randomly selected plants of six different populations of L. nervosum varieties, including var. nervosum and var. bungei, were studied for morphological and genetic variability as well as population structure. Analysis of variance (ANOVA) did not show significant morphological difference between populations. PCA as well as PCA biplot confirmed that some morphological traits have taxonomic value. UPGMA clustering separated the populations of varieties in two distinct clusters, indicating degrees of morphological differentiation between them. Furthermore, UPGMA confirmed the variability in morphological characters within populations. Neighbor Joining tree and Neighbor-Net analysis of ISSR data revealed inter- and intrapopulation genetic variability. STRUCTURE plot revealed allelic difference of these varieties and some degree of intervarietal gene flow. K-means clustering showed the fragmentation of populations in support of AMOVA test, which revealed significant genetic difference among them. In general, obtained results confirmed the alternation of taxonomic level of L. bungei to the variety of L. nervosum.
神经林是可以与L。 产下可育的后代。从保护和育种的角度来看,对亚麻野生亲缘关系的遗传多样性分析具有重要意义。在本研究中,从六个不同群体的神经乳杆菌品种中随机选择了55株植物,其中包括变种。 神经根和变种。 对bungi的形态和遗传变异以及种群结构进行了研究。方差分析(ANOVA)没有显示种群之间的显著形态差异。主成分分析和主成分分析双谱图证实了某些形态性状具有分类学价值。UPGMA聚类将品种群体分为两个不同的聚类,表明它们之间的形态分化程度。此外,UPGMA证实了种群内形态特征的变异性。ISSR数据的邻居连接树和邻居网分析揭示了群体间和群体内的遗传变异。结构图揭示了这些品种的等位基因差异和一定程度的品种间基因流动。K-means聚类显示了支持AMOVA检验的种群的破碎化,揭示了它们之间的显著遗传差异。总的来说,所获得的结果证实了L。 对L。 神经系统。
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引用次数: 0
Trichomes micromorphology and cytological investigation on Acinos alpinus subsp. meridionalis in Algeria 高山不动产酸亚种毛状体微形态及细胞学研究。阿尔及利亚的子午线
IF 0.3 Q4 PLANT SCIENCES Pub Date : 2018-04-10 DOI: 10.5281/zenodo.1216175
N. Saouli, Hafsa Rahmani, Hadjer Smara, N. Khalfallah
Anatomical and karyological features were investigated in four populations of Acinos alpinus subsp. meridionalis (Nyman) P. Ball., an Ibero-Maghreban species. Plant material was collected in the field during flowering stage, in contrasting bioclimatic conditions. Fresh material was used for analysis of trichomes morphology with light microscopy. Young meristems and flower buds were fixed for karyological analysis. Two types of capitate glandular, one type of peltate glandular, and four types of non-glandular trichomes were observed on different parts of the plants, including the stems, leaves and flowers. Trichomes distribution and density showed variability among organs, but no intraspecific variability was found. The chromosome number 2n = 2x = 18 is reported for the first time for Algerian populations. Furthermore, aberrant meiotic behavior in the form of cytomixis was observed in one population of A. alpinus subsp. meridionalis – cytoplasmic strand was formed between more than two cells at different stages of meiosis. Other types of abnormalities such as lagging chromosomes, and formation of triads with microcytes were also discovered. Results are discussed in comparison with literature.
对4个居群的解剖和核学特征进行了研究。子午线(尼曼)p。伊比利亚-马格里布的一种。植物材料采集于花期,在不同的生物气候条件下。用新鲜材料对毛状体形态进行光镜分析。将幼分生组织和花蕾固定,进行核学分析。在茎、叶、花的不同部位均有2种头状毛状体、1种盾状毛状体和4种非腺状毛状体。毛状体分布和密度在各器官间存在差异,但种内无差异。染色体编号2n = 2x = 18在阿尔及利亚人群中首次报道。此外,在一个种群中观察到以巨细胞分裂形式出现的异常减数分裂行为。子午线-细胞质链在两个以上的细胞之间在减数分裂的不同阶段形成。其他类型的异常,如滞后染色体,形成与小细胞三联体也被发现。结果与文献进行了对比讨论。
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引用次数: 0
Distribution, morphology, anatomy and histochemistry of Crepidium acuminatum 尖鳞毛的分布、形态、解剖和组织化学
IF 0.3 Q4 PLANT SCIENCES Pub Date : 2018-03-11 DOI: 10.5281/ZENODO.1195691
S. J. Adams, T. S. Kumar, G. Muthuraman, A. Majeed
Crepidium acuminatum is largely confine to the Himalayan region although it has been reported in South India, where its presence distribution needs to be critically studied. This study describes the morphological, anatomical and histochemical aspects of the materials of the authenticated species collected from Himalayas. This study also highlights the features of histochemistry and anatomy that should be used for the correct identification and authentication of C. acuminatum, especially because of the therapeutic importance of the species and its possible adulteration by other orchids.
尖锐Crepidium acuminatum主要局限于喜马拉雅地区,尽管在南印度也有报道,但其存在分布需要进行严格研究。本研究描述了从喜马拉雅山脉收集的鉴定物种的形态、解剖和组织化学方面的材料。本研究还强调了组织化学和解剖特征,应用于正确鉴定和鉴定,特别是因为该物种的治疗重要性和它可能被其他兰花掺假。
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引用次数: 4
Seed shape quantification in the order Cucurbitales 南瓜目种子形状的定量
IF 0.3 Q4 PLANT SCIENCES Pub Date : 2018-02-17 DOI: 10.5281/ZENODO.1174871
E. Cervantes, J. J. Gómez
Seed shape quantification in diverse species of the families belonging to the order Cucurbitales is done based on the comparison of seed images with geometric figures. Quantification of seed shape is a useful tool in plant description for phenotypic characterization and taxonomic analysis. J index gives the percent of similarity of the image of a seed with a geometric figure and it is useful in taxonomy for the study of relationships between plant groups. Geometric figures used as models in the Cucurbitales are the ovoid, two ellipses with different x/y ratios and the outline of the Fibonacci spiral. The images of seeds have been compared with these figures and values of J index obtained. The results obtained for 29 species in the family Cucurbitaceae support a relationship between seed shape and species ecology. Simple seed shape, with images resembling simple geometric figures like the ovoid, ellipse or the Fibonacci spiral, may be a feature in the basal clades of taxonomic groups.
通过种子图像与几何图形的比较,对葫芦目不同科植物的种子形状进行了定量分析。种子形状的定量是植物表型鉴定和分类分析的有效工具。J指数给出了种子图像与几何图形的相似度百分比,它在植物类群间关系的分类学研究中很有用。在葫芦族中用作模型的几何图形是卵形,两个具有不同x/y比率的椭圆和斐波那契螺旋的轮廓。并将种子图像与所得到的J指数进行了比较。对瓜科29种植物的研究结果支持种子形状与物种生态之间的关系。简单的种子形状,其图像类似于简单的几何图形,如卵形、椭圆或斐波那契螺旋,可能是分类类群的基础分支的一个特征。
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引用次数: 12
New and dangerous bacterial disease of oleander Pseudomonas savastanoi pv. Nerii in greenhouses of ukraine 夹竹桃假单胞菌的新危细菌性病害。Nerii在乌克兰的温室
IF 0.3 Q4 PLANT SCIENCES Pub Date : 2018-01-01 DOI: 10.5281/ZENODO.2641748
M. M. Kluchevich, P. Chumak, S. Vigera
Object: Study of symptoms and etiology of oleander tuberculosis causative agents and determination of causes of their appearance in conditions of the closed ground of the greenhouses of Ukraine. Methods: Samples for research were selected in greenhouses of Botanical Garden named after acad. A. V. Fomin from different kinds and forms of oleander (Nerium oleander L.). The microbiological and phytopathological analysis was carried out after K. I. Beltyukova. Biolog GN MicroPlate system was used to identify isolated bacterial strains. Calculations for bacteria identification in relation to genus, species and other taxonomic units are based on the similarity index. Results: On oleander plants, the greatest disease progress shows itself on the branches of the second year of development. Up to 5 bacterial nodes of different sizes can be formed on one branch. Galls can be formed on the leaves both on the upper (62.1%) and lower (37.9%) sides. Bacteria actively spread with water from branches and leaves, especially when spraying plants from a hose. The infection degree of different varieties of oleander was not the same. Such varieties and forms as of Nerium oleander "Album", Nerium oleander "Album Plenum" suffer from bacteriosis the most. Conclusions: For the first time in Ukraine tuberculosis Pseudomonas savastanoi pv. nerii was detected on oleander (Nerium oleander L.). The infection degree of different varieties of oleander was not the same. Nerium oleander "Album", Nerium oleander "Album Plenum", are the most severely leased, Nerium oleander "Flavescens", Nerium oleander "Lacteum" are leased to the lesser extent. The spread of the disease is favored by some methods of this group of plants growing: pruning plants, branches, and leaves bruise.
目的:研究乌克兰温室封闭场地条件下夹竹桃结核病原菌的症状、病原学及其产生原因。方法:选取不同种类、不同形态夹竹桃(Nerium oleander L.)在以acad. a.v. Fomin命名的植物园温室内进行研究。在k.i. Beltyukova后进行微生物学和植物病理学分析。采用biologgn MicroPlate系统对分离菌株进行鉴定。计算细菌鉴定的属,种和其他分类单位是基于相似性指数。结果:夹竹桃植株在发育第二年的枝条上病害进展最快。在一个分枝上最多可形成5个不同大小的细菌结。叶片上侧(62.1%)和下侧(37.9%)均可形成瘿瘤。细菌会随着树枝和叶子上的水而传播,尤其是在用软管喷洒植物时。不同品种夹竹桃的侵染程度不同。夹竹桃“Album”、夹竹桃“Album全会”等品种和形态最易患细菌病。结论:乌克兰首次检出沙斯塔假单胞菌。夹竹桃(Nerium oleander L.)上检测到夹竹桃属植物。不同品种夹竹桃的侵染程度不同。夹竹桃“Album”、夹竹桃“Album全会”租期最严重,夹竹桃“Flavescens”、夹竹桃“Lacteum”租期较轻。疾病的传播是通过一些有利于这类植物生长的方法:修剪植物的枝干,擦伤叶子。
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引用次数: 0
Floral anatomy and micromorphology of Hyacinthoides italica (L.) Rothm. A case of complete stachyospory in Asparagaceæ 意大利风信子的花解剖与显微形态Rothm。天门冬科完全链孢子一例
IF 0.3 Q4 PLANT SCIENCES Pub Date : 2018-01-01 DOI: 10.5281/ZENODO.1313005
Julie Zalko, T. Deroin
Floral vasculature and gynoecium architecture were studied in Hyacinthoides italica (L.) Rothm. Each locule shelters two ovules inserted basally and surprisingly supplied by axis bundles only, while lateral bundles of the carpels branch out into strands along the epidermises of septal nectaries. We brought then to the fore an unexpected trade-off between the supply of upper ovules in H. non-scripta and that of secretory tissues in the studied species. Moreover, a new pattern of septal nectary is described, with outer and inner cavities.
对意大利风信子(Hyacinthoides italica)花的维管系统和雌蕊结构进行了研究。Rothm。每个室包住两个胚珠,胚珠基部着生,令人惊讶的是,胚珠仅由轴束提供,而心皮的侧束沿着隔蜜腺表皮分支成股状。我们将非scripta上胚珠的供应与所研究物种的分泌组织之间的意想不到的权衡带到前台。此外,还描述了一种新的室间隔蜜腺模式,具有外腔和内腔。
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引用次数: 2
Spatiotemporal differentiation and distribution patterns of the genus Plantago L. (Plantaginaceae) in the plain part of Ukraine during the Allerød–Holocene 全新世乌克兰平原车前草属植物的时空分异与分布格局
IF 0.3 Q4 PLANT SCIENCES Pub Date : 2018-01-01 DOI: 10.5281/ZENODO.1319359
L. Bezusko, Z. Tsymbalyuk, S. Mosyakin
Results of an analysis and generalization of data on participation of pollen grains of Plantago species (Plantaginaceae) in the collective pollen floras of the Allerod–Holocene deposits of the forest, forest-steppe and steppe zones of Ukraine are considered. Five model species (P. lanceolata, P. major, P. maxima, P. media and P. urvillei) were identified. It has been found that each of model species had its own individual history of distribution in the plain part of Ukraine during the Allerod–Holocene, which was caused by natural and anthropogenic factors. For pollen grains of each model taxon of Plantago, a set of quantitative and qualitative diagnostic characters has been provided, which should be taken into account in their identification during the palynological studies of the Pleistocene–Holocene deposits of Ukraine and adjacent territories.
本文对乌克兰森林、森林-草原和草原带变冷-全新世沉积物中车前草属(Plantaginaceae)花粉粒参与的数据进行了分析和归纳。鉴定出杉木、大杉木、大杉木、中等杉木和野杉木5种模式种。研究发现,在变冷-全新世时期,每一种模式种在乌克兰平原地区都有其独特的分布历史,这是自然和人为因素共同作用的结果。对于车前草各模式分类群的花粉粒,提供了一套定量和定性的诊断特征,在乌克兰及邻近地区更新世-全新世沉积物的孢粉学研究中应予以考虑。
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引用次数: 1
Taxonomic diversity of Astragalus L. in alpine and sub-alpine zones in talesh mountains, northwest iran 伊朗西北部塔雷什山区高山和亚高山地区黄芪的分类多样性
IF 0.3 Q4 PLANT SCIENCES Pub Date : 2018-01-01 DOI: 10.5281/zenodo.2641727
Mahmoud Bidarlord, F. Ghahremaninejad, A. Maassoumi
The largest genus in Iran has investigated in Talesh Mountains. These mountains are located along the transition area between Irano-Turanian and Hyrcanian phytochoria in the northwest Iran. Astragalus with 61 species belong to 20 sections represents in Talesh Mountains alpine and subalpine zones, 21 are endemic, and 27 taxa either in the country’s or the state’s Red Lists of categories. The results provides checklist of Astragalus species, a taxonomic comments for some species, also recorded some species from NW Iran and report new localities for recently introduced Astragalus species.
在Talesh山脉调查了伊朗最大的属。这些山脉位于伊朗西北部的伊朗-图拉尼亚和赫卡尼亚植物群落之间的过渡地区。黄芪61种,分属Talesh山脉高寒和亚高寒地区20个类群,21个为特有种,27个分类群被列入国家或州的红色分类名录。结果提供了黄芪属植物名录,对部分物种进行了分类评述,并记录了伊朗西北部部分黄芪属植物,报告了近期引进黄芪属植物的新分布地。
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引用次数: 3
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Modern Phytomorphology
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