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The mechanism of 14-3-3η in thyroxine induced mitophagy in cardiomyocytes 14-3-3η在甲状腺素诱导心肌细胞有丝分裂中的作用机制
IF 4.1 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-15 DOI: 10.1016/j.mce.2024.112271
Yalan Cui , Yan Zhang , Songsong Dai , Sha Wan , Heng Guan , Decai Wang , Beifang Jin , Wenping Xiao , Fang Liu

Hyperthyroidism is becoming increasingly important as an independent risk factor for cardiovascular disease, eventually resulting in cardiac hypertrophy and heart failure. The 14-3-3 protein family subtypes regulate many cellular processes in eukaryotes by interacting with a diverse array of client proteins. Considering that the 14-3-3η protein protects cardiomyocytes by affecting mitochondrial function, exploring the biological influence and molecular mechanisms by which 14-3-3η alleviates the cardiac hypertrophy of hyperthyroidism is imperative. In vivo and in vitro, RT-PCR, Western blot, and Mitochondrial tracking assay were performed to understand the molecular mechanism of thyroxine-induced cardiomyocyte hypertrophy. HE staining, transmission electron microscopy, and immunofluorescence were used to observe intuitively changes of hearts and cardiomyocytes. The in vivo and in vitro results indicated that overexpression of the 14-3-3η ameliorated thyroxine-induced cardiomyocyte hypertrophy, whereas knockdown of the 14-3-3η protein aggravated thyroxine-induced cardiomyocyte hypertrophy. Additionally, overexpression of the 14-3-3η protein reduces thyroxine-induced mitochondrial damage and mitophagy in cardiomyocytes. Overexpression of 14-3-3η protein improves excessive mitophagy in the myocardium caused by thyroxine and thus prevents cardiac hypertrophy.

甲状腺功能亢进症作为心血管疾病的一个独立风险因素正变得越来越重要,它最终会导致心脏肥大和心力衰竭。14-3-3 蛋白家族亚型通过与各种客户蛋白相互作用,调节真核生物的许多细胞过程。考虑到 14-3-3η 蛋白通过影响线粒体功能来保护心肌细胞,探索 14-3-3η 减轻甲亢性心肌肥厚的生物学影响和分子机制势在必行。为了了解甲状腺素诱导心肌细胞肥大的分子机制,研究人员分别在体内和体外进行了 RT-PCR、Western 印迹和线粒体追踪检测。通过 HE 染色、透射电镜和免疫荧光观察心脏和心肌细胞的直观变化。体内和体外研究结果表明,过表达 14-3-3η 蛋白可改善甲状腺素诱导的心肌细胞肥大,而敲除 14-3-3η 蛋白则会加重甲状腺素诱导的心肌细胞肥大。此外,过表达 14-3-3η 蛋白可减少甲状腺素诱导的心肌细胞线粒体损伤和有丝分裂。过量表达 14-3-3η 蛋白可改善甲状腺素导致的心肌细胞过度有丝分裂,从而防止心肌肥大。
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引用次数: 0
The role of nitric oxide and neuroendocrine system in pain generation 一氧化氮和神经内分泌系统在疼痛产生中的作用。
IF 4.1 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-14 DOI: 10.1016/j.mce.2024.112270
Aayush Gupta , Maja Vejapi , Nebojsa Nick Knezevic

Previous studies have indicated a complex interplay between the nitric oxide (NO) pain signaling pathways and hormonal signaling pathways in the body. This article delineates the role of nitric oxide signaling in neuropathic and inflammatory pain generation and subsequently discusses how the neuroendocrine system is involved in pain generation. Hormonal systems including the hypothalamic-pituitary axis (HPA) generation of cortisol, the renin-angiotensin-aldosterone system, calcitonin, melatonin, and sex hormones could potentially contribute to the generation of nitric oxide involved in the sensation of pain. Further research is necessary to clarify this relationship and may reveal therapeutic targets involving NO signaling that alleviate neuropathic and inflammatory pain.

以往的研究表明,一氧化氮(NO)疼痛信号通路与体内荷尔蒙信号通路之间存在复杂的相互作用。本文阐述了一氧化氮信号在神经性和炎症性疼痛产生中的作用,随后讨论了神经内分泌系统如何参与疼痛的产生。荷尔蒙系统包括下丘脑-垂体轴(HPA)产生的皮质醇、肾素-血管紧张素-醛固酮系统、降钙素、褪黑激素和性荷尔蒙,这些系统都有可能促进一氧化氮的产生,从而影响疼痛的感觉。有必要开展进一步的研究来澄清这种关系,并揭示涉及一氧化氮信号转导的治疗目标,以减轻神经性和炎症性疼痛。
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引用次数: 0
Oral supplementation with resveratrol improves hormonal profile and increases expression of genes associated with thermogenesis in oophorectomy mice 口服补充白藜芦醇可改善卵巢切除小鼠的荷尔蒙状况,并增加与产热相关的基因表达。
IF 4.1 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-11 DOI: 10.1016/j.mce.2024.112268
Fhelício Sampaio Viana , Juliana Andrade Pereira , Thaísa Soares Crespo , Lílian Betânia Reis Amaro , Eliezer Francisco Rocha , Alice Crespo Fereira , Deborah de Farias Lelis , Thaís de Oliveira Faria Baldo , Marcelo Perim Baldo , Sérgio Henrique Sousa Santos , João Marcus Oliveira Andrade

Menopause causes important bodily and metabolic changes, which favor the increased occurrence of cardiovascular diseases, obesity, diabetes, and osteoporosis. Resveratrol exerts proven effects on body metabolism, improving glucose and lipid homeostasis and reducing inflammation and oxidative stress in various organs and tissues. Accordingly, this study evaluates the effects of resveratrol supplementation on the expression of markers associated with thermogenesis in brown adipose tissue, and on the body, metabolic and hormonal parameters of female mice submitted to bilateral oophorectomy. Eighteen female mice were randomized into three groups: G1: control (CONTROL), G2: oophorectomy (OOF), and G3: oophorectomy + resveratrol (OOF + RSV); the animals were kept under treatment for twelve weeks, being fed a standard diet and treated with resveratrol via gavage. Body, biochemical, hormonal, and histological parameters were measured; in addition to the expression of markers associated with thermogenesis in brown adipose tissue. The results showed that animals supplemented with resveratrol showed reduced body weight and visceral adiposity, in addition to glucose, total cholesterol, and triglyceride levels; decreased serum FSH levels and increased estrogen levels were observed compared to the OOF group and mRNA expression of PRDM16, UCP1, and SIRT3 in brown adipose tissue. The findings of this study suggest the important role of resveratrol in terms of improving body, metabolic, and hormonal parameters, as well as modulating markers associated with thermogenesis in brown adipose tissue of female mice submitted to oophorectomy.

更年期会引起重要的身体和新陈代谢变化,增加心血管疾病、肥胖症、糖尿病和骨质疏松症的发病率。白藜芦醇对人体新陈代谢、改善血糖和血脂平衡、减少各器官和组织的炎症和氧化应激有显著效果。因此,本研究评估了补充白藜芦醇对棕色脂肪组织产热相关标记物表达的影响,以及对接受双侧输卵管切除术的雌性小鼠身体、代谢和激素参数的影响。18 只雌性小鼠被随机分为三组:G1组:对照组(CONTROL),G2组:卵巢切除术组(OOF),G3组:卵巢切除术+白藜芦醇组(OOF+RSV);这些动物被饲养十二周,以标准饮食喂养,并通过灌胃的方式接受白藜芦醇治疗。对动物的身体、生化、激素和组织学参数进行了测量,此外还测量了棕色脂肪组织中与产热相关的标记物的表达。结果表明,与 OOF 组相比,补充白藜芦醇的动物体重和内脏脂肪含量降低,葡萄糖、总胆固醇和甘油三酯水平也有所降低;血清 FSH 水平降低,雌激素水平升高;棕色脂肪组织中 PRDM16、UCP1 和 SIRT3 的 mRNA 表达量也有所降低。该研究结果表明,白藜芦醇在改善输卵管切除术雌性小鼠的身体、代谢和激素参数,以及调节棕色脂肪组织中与产热相关的标记物方面具有重要作用。
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引用次数: 0
Epigenetic impact of hypothyroidism on the functional differentiation of the mammary gland in rats 甲状腺功能减退症对大鼠乳腺功能分化的表观遗传学影响
IF 4.1 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-09 DOI: 10.1016/j.mce.2024.112267
Fiorella Campo Verde Arbocco , Lourdes Inés Pascual , Daiana García , Irina Ortiz , Carlos Gamarra-Luques , Rubén Walter Carón , María Belén Hapon

Mammary gland (MG) lactogenic differentiation involves epigenetic mechanisms. We have previously shown that hypothyroidism (HypoT) alters the MG transcriptome in lactation. However, the role of thyroid hormones (T3 and T4 a. k.a. THs) in epigenetic differentiation of MG is still unknown. We used a model of post-lactating HypoT rats to study in MG: a) Methylation and expression level of Gata3, Elf5, Stat6, Stat5a, Stat5b; b) Expression of Lalba, IL-4Rα and Ncoa1 mRNA; c) Histone H3 acetylation and d) Estrogen and progesterone concentration in serum. HypoT increases the estrogen serum level, decreases the progesterone level, promotes methylation of Stat5a, Stat5b and Stat6, decreasing their mRNA level and of its target genes (Lalba and IL-4Rα) and increases the Ncoa1 mRNA expression and histone H3 acetylation level. Our results proved that HypoT alters the post-lactation MG epigenome and could compromise mammary functional differentiation.

乳腺(MG)的泌乳分化涉及表观遗传机制。我们之前已经证明,甲状腺功能减退症(HypoT)会改变哺乳期乳腺的转录组。然而,甲状腺激素(T3和T4,又称THs)在MG表观遗传分化中的作用尚不清楚。我们利用泌乳后低TH大鼠模型研究了MG:a) Gata3、Elf5、Stat6、Stat5a、Stat5b的甲基化和表达水平;b) Lalba、IL-4Rα和Ncoa1 mRNA的表达;c) 组蛋白H3乙酰化;d) 血清中雌激素和孕酮的浓度。HypoT增加了血清中的雌激素水平,降低了孕酮水平,促进了Stat5a、Stat5b和Stat6的甲基化,降低了它们的mRNA水平及其靶基因(Lalba和IL-4Rα)的mRNA水平,增加了Ncoa1 mRNA的表达和组蛋白H3的乙酰化水平。我们的研究结果证明,HypoT改变了泌乳后MG表观基因组,并可能影响乳腺功能分化。
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引用次数: 0
The role of nitric oxide and hormone signaling in chronic stress, anxiety, depression and post-traumatic stress disorder 一氧化氮和激素信号在慢性压力、焦虑、抑郁和创伤后应激障碍中的作用。
IF 4.1 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-06 DOI: 10.1016/j.mce.2024.112266
Tamara Jankovic , Marko Bogicevic , Nebojsa Nick Knezevic

This paper provides a summary of the role of nitric oxide (NO) and hormones in the development of chronic stress, anxiety, depression, and post-traumatic stress disorder (PTSD). These mental health conditions are prevalent globally and involve complex molecular interactions. Although there is a significant amount of research and therapeutic options available, the underlying mechanisms of these disorders are still not fully understood. The primary pathophysiologic processes involved in chronic stress, anxiety, depression, and PTSD include dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis, the intracellular influence of neuronal nitric oxide synthase (nNOS) on transcription factors, an inflammatory response with the formation of nitrergic oxidative species, and reduced serotonergic transmission in the dorsal raphe nucleus. Despite the extensive literature on this topic, there is a great need for further research to clarify the complexities inherent in these pathways, with the primary aim of improving psychiatric care.

本文概述了一氧化氮(NO)和激素在慢性压力、焦虑、抑郁和创伤后应激障碍(PTSD)发展过程中的作用。这些精神疾病在全球普遍存在,涉及复杂的分子相互作用。虽然目前已有大量的研究和治疗方案,但人们对这些疾病的基本机制仍不完全了解。慢性压力、焦虑、抑郁和创伤后应激障碍所涉及的主要病理生理过程包括下丘脑-垂体-肾上腺(HPA)轴的失调、细胞内神经元一氧化氮合酶(nNOS)对转录因子的影响、硝化氧化物形成的炎症反应以及背侧剑突核5-羟色胺能传导的降低。尽管有关这一主题的文献很多,但仍亟需进一步研究,以澄清这些途径中固有的复杂性,其主要目的是改善精神病治疗。
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引用次数: 0
Pregnancy, abortion, and birth control methods’ complicity with breast cancer occurrence 怀孕、流产和节育方法与乳腺癌发生的共谋关系。
IF 4.1 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-03 DOI: 10.1016/j.mce.2024.112264
Katarzyna Rakoczy , Justyna Kaczor , Adam Sołtyk , Laura Jonderko , Mikołaj Sędzik , Julia Lizon , Anna Lewandowska , Małgorzata Saczko , Julita Kulbacka

Reproductive factors play significantly important roles in determining the breast cancer (BC) risk. The impact of pregnancy, abortion, and birth control methods on tumor development remains unclear. It has been found that early full-term pregnancies in young women can lower their lifetime risk of developing the type of cancer in question. However, having a first full-term pregnancy at an older age can increase this risk. The relationship between pregnancy and breast cancer (BC) is, however, much more complicated. Both induced and spontaneous abortions lead to sudden changes in hormonal balance, which could cause different effects on sensitive breast epithelial cells, making abortion a potential risk factor for breast cancer. The influence of hormonal contraception on carcinogenesis is not comprehensively understood, and therefore, more exhaustive analysis of existing data and further investigation is needed. This review explores how the mentioned reproductive factors affect the risk of breast cancer (BC), focusing on the molecular mechanisms that contribute to its complexity. By comprehending this intricate network of relationships, we can develop new strategies for predicting and treating the disease.

生殖因素在决定乳腺癌(BC)风险方面起着非常重要的作用。怀孕、流产和节育方法对肿瘤发生的影响仍不清楚。研究发现,年轻女性早期足月妊娠可降低其一生中罹患相关类型癌症的风险。然而,在年龄较大时首次足月妊娠则会增加这种风险。然而,怀孕与乳腺癌(BC)之间的关系要复杂得多。人工流产和自然流产都会导致荷尔蒙平衡的突然变化,从而对敏感的乳腺上皮细胞产生不同的影响,使流产成为乳腺癌的潜在危险因素。激素避孕对致癌的影响尚未得到全面了解,因此需要对现有数据进行更详尽的分析和进一步研究。本综述探讨了上述生殖因素如何影响乳腺癌(BC)的发病风险,重点关注导致其复杂性的分子机制。通过了解这一错综复杂的关系网络,我们可以开发出预测和治疗该疾病的新策略。
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引用次数: 0
A brief and updated introduction to the neuroendocrine system of crustaceans 甲壳动物神经内分泌系统的最新简要介绍。
IF 4.1 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-01 DOI: 10.1016/j.mce.2024.112265
Leonardo Airton Ressel Simões , Rafaella Sanfelice Normann , J. Sook Chung , Anapaula Sommer Vinagre

The neuroendocrine system of crustaceans is complex and regulates many processes, such as development, growth, reproduction, osmoregulation, behavior, and metabolism. Once stimulated, crustaceans’ neuroendocrine tissues modulate the release of monoamines, ecdysteroids, and neuropeptides that can act as hormones or neurotransmitters. Over a few decades, research has unraveled some mechanisms governing these processes, substantially contributing to understanding crustacean physiology. More aspects of crustacean neuroendocrinology are being comprehended with molecular biology, transcriptome, and genomics analyses. Hence, these studies will also significantly enhance the ability to cultivate decapods, such as crabs and shrimps, used as human food sources. In this review, current knowledge on crustacean endocrinology is updated with new findings about crustacean hormones, focusing mainly on the main neuroendocrine organs and their hormones and the effects of these molecules regulating metabolism, growth, reproduction, and color adaptation. New evidence about vertebrate-type hormones found in crustaceans is included and discussed. Finally, this review may assist in understanding how the emerging chemicals of environmental concern can potentially impair and disrupt crustacean's endocrine functions and their physiology.

甲壳类动物的神经内分泌系统十分复杂,调节着许多过程,如发育、生长、繁殖、渗透调节、行为和新陈代谢。一旦受到刺激,甲壳类动物的神经内分泌组织就会调节单胺、蜕皮激素和神经肽的释放,这些物质可作为激素或神经递质发挥作用。几十年来,研究已经揭示了这些过程的一些机制,大大有助于人们了解甲壳动物的生理学。通过分子生物学、转录组和基因组学分析,甲壳动物神经内分泌学的更多方面正在被理解。因此,这些研究也将大大提高养殖蟹和虾等作为人类食物来源的十足目动物的能力。本综述更新了甲壳动物内分泌学的现有知识,介绍了有关甲壳动物激素的新发现,主要侧重于主要神经内分泌器官及其激素,以及这些分子对新陈代谢、生长、繁殖和颜色适应的调节作用。本综述还包括并讨论了在甲壳类动物中发现的脊椎动物类激素的新证据。最后,这篇综述可能有助于理解新出现的环境问题化学品如何潜在地损害和干扰甲壳类动物的内分泌功能及其生理机能。
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引用次数: 0
CircZNF609 inhibits miR-150-5p to promote high glucose-induced damage to retinal microvascular endothelial cells CircZNF609抑制miR-150-5p,促进高血糖诱导的视网膜微血管内皮细胞损伤。
IF 4.1 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-04-27 DOI: 10.1016/j.mce.2024.112261
Jing Gao , Chenfei Wang , Jie Zhang , Zulifeiya Shawuti , Siyao Wang , Cunhua Ma , Juan Wang

Hyperglycemia is a key contributor to diabetic macrovascular and ocular complications. It triggers a cascade of cellular damage, particularly in the retinal microvascular endothelial cells (RMECs). However, the underlying molecular mechanisms remain only partially understood. This study hypothesizes that CircZNF609 plays a pivotal role in mediating high glucose-induced damage in RMECs by modulating miR-150-5p and its downstream target genes, thereby affecting cellular survival, apoptosis, and oxidative stress. Gene expression datasets (GSE193974 and GSE160308) and clinical samples were used to investigate the expression levels of CircZNF609 and its interaction with miR-150-5p in the context of diabetic retinopathy (DR). Our results demonstrate that CircZNF609 is upregulated in both peripheral blood stem cells from DR patients and high glucose-stimulated hRMECs. Functional experiments reveal that silencing CircZNF609 improves cell viability, reduces apoptosis, inhibits tube formation, and modulates oxidative stress markers, whereas CircZNF609 overexpression exacerbates these effects. Moreover, miR-150-5p, a microRNA, was found to be negatively regulated by CircZNF609 and downregulated in DR. Its overexpression mitigates high glucose-induced cell injury. Our findings suggest a novel mechanism whereby CircZNF609 exacerbates high glucose-induced endothelial cell damage by sponging miR-150-5p, implicating the CircZNF609/miR-150-5p axis as a potential therapeutic target in diabetic retinopathy.

高血糖是导致糖尿病大血管和眼部并发症的关键因素。它会引发一连串的细胞损伤,尤其是视网膜微血管内皮细胞(RMECs)。然而,人们对其潜在的分子机制仍只有部分了解。本研究假设 CircZNF609 通过调节 miR-150-5p 及其下游靶基因,从而影响细胞存活、凋亡和氧化应激,在介导高血糖诱导的 RMECs 损伤中发挥关键作用。我们利用基因表达数据集(GSE193974和GSE160308)和临床样本研究了糖尿病视网膜病变(DR)背景下CircZNF609的表达水平及其与miR-150-5p的相互作用。我们的研究结果表明,CircZNF609在DR患者的外周血干细胞和高糖刺激的hRMECs中均上调。功能实验显示,沉默CircZNF609可提高细胞活力、减少细胞凋亡、抑制管形成并调节氧化应激标记,而过表达CircZNF609则会加剧这些影响。此外,研究还发现,微RNA miR-150-5p受CircZNF609负调控,并在DR中下调。它的过表达可减轻高糖诱导的细胞损伤。我们的研究结果表明了一种新的机制,即CircZNF609通过疏导miR-150-5p来加剧高糖诱导的内皮细胞损伤,这意味着CircZNF609/miR-150-5p轴是糖尿病视网膜病变的潜在治疗靶点。
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引用次数: 0
Exogenous thyroxine increases cardiac GLUT4 translocation in insulin resistant OLETF rats 外源性甲状腺素可增加胰岛素耐受性 OLETF 大鼠心脏 GLUT4 转位
IF 4.1 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-04-26 DOI: 10.1016/j.mce.2024.112254
Dora A. Mendez , José G. Soñanez-Organis , Xue Yang , Guillermo Vazquez-Anaya , Akira Nishiyama , Rudy M. Ortiz

During insulin resistance, the heart undergoes a metabolic shift in which fatty acids (FA) account for roughly about 99% of the ATP production. This metabolic shift is indicative of impaired glucose metabolism. A shift in FA metabolism with impaired glucose tolerance can increase reactive oxygen species (ROS), lipotoxicity, and mitochondrial dysfunction, ultimately leading to cardiomyopathy. Thyroid hormones (TH) may improve the glucose intolerance by increasing glucose reabsorption and metabolism in peripheral tissues, but little is known on its effects on cardiac tissue during insulin resistance. In the present study, insulin resistant Otsuka Long Evans Tokushima Fatty (OLETF) rats were used to assess the effects of exogenous thyroxine (T4) on glucose metabolism in cardiac tissue. Rats were assigned to four groups: (1) lean, Long Evans Tokushima Otsuka (LETO; n=6), (2) LETO + T4 (8 μg/100 g BM/d × 5 wks; n = 7), (3) untreated OLETF (n = 6), and (4) OLETF + T4 (8 μg/100 g BM/d × 5 wks; n = 7). T4 increased GLUT4 gene expression by 85% in OLETF and increased GLUT4 protein translocation to the membrane by 294%. Additionally, T4 increased p-AS160 by 285%, phosphofructokinase-1 (PFK-1) mRNA, the rate limiting step in glycolysis, by 98% and hexokinase II by 64% in OLETF. T4 decreased both CPT2 mRNA and protein expression in OLETF. The results suggest that exogenous T4 has the potential to increase glucose uptake and metabolism while simultaneously reducing fatty acid transport in the heart of insulin resistant rats. Thus, L-thyroxine may have therapeutic value to help correct the impaired substrate metabolism associated with diabetic cardiomyopathy.

在胰岛素抵抗期间,心脏会发生代谢转变,其中脂肪酸(FA)约占 ATP 生成量的 99%。这种代谢转变表明葡萄糖代谢受损。随着糖耐量受损,脂肪酸代谢的转变会增加活性氧(ROS)、脂肪毒性和线粒体功能障碍,最终导致心肌病。甲状腺激素(TH)可通过增加外周组织对葡萄糖的重吸收和新陈代谢来改善糖耐量不全,但其对胰岛素抵抗期间心脏组织的影响却鲜为人知。本研究使用胰岛素抵抗的大冢长伊万德岛脂肪大鼠(OLETF)来评估外源性甲状腺素(T4)对心脏组织葡萄糖代谢的影响。大鼠被分为四组:(1) 瘦长埃文斯德岛大鼠(LETO;n=6),(2) LETO + T4(8 μg/100 g BM/d × 5 周;n = 7),(3) 未处理的 OLETF(n = 6),(4) OLETF + T4(8 μg/100 g BM/d × 5 周;n = 7)。在 OLETF 中,T4 使 GLUT4 基因表达增加 85%,使 GLUT4 蛋白转位到膜上增加 294%。此外,在 OLETF 中,T4 使 p-AS160 增加了 285%,使糖酵解过程中的限速步骤磷酸果糖激酶-1(PFK-1)mRNA 增加了 98%,使己糖激酶 II 增加了 64%。T4 降低了 CPT2 mRNA 和蛋白在 OLETF 中的表达。结果表明,外源性 T4 有可能增加胰岛素抵抗大鼠心脏的葡萄糖摄取和新陈代谢,同时减少脂肪酸转运。因此,左旋甲状腺素可能具有治疗价值,有助于纠正与糖尿病心肌病相关的底物代谢障碍。
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引用次数: 0
FGF2 promotes the proliferation of injured granulosa cells in premature ovarian failure via Hippo-YAP signaling pathway FGF2 通过 Hippo-YAP 信号通路促进卵巢早衰中受伤颗粒细胞的增殖
IF 4.1 3区 医学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-04-24 DOI: 10.1016/j.mce.2024.112248
Feiyan Cheng , Jingyuan Wang , Rongli Wang , Rumeng Pan , Zhiwei Cui , Lijun Wang , Lihui Wang , Xinyuan Yang

Young women undergoing anticancer treatment are at risk of premature ovarian failure (POF). Endometrial-derived stem cells (EnSCs) have demonstrated significant therapeutic potential for treating ovarian insufficiency, although the underlying mechanisms remain to be fully understood. This study aims to further investigate the therapeutic effects of EnSCs, particularly through the paracrine action of fibroblast growth factor 2 (FGF2), on POF. The findings show that exogenous FGF2 enhances the survival of ovarian granulosa cells damaged by cisplatin. FGF2 stimulates the proliferation of these damaged cells by suppressing the Hippo signaling pathway and activating YAP expression. In vivo experiments also revealed that FGF2 treatment significantly improves ovarian reserve and endocrine function in mice with POF. These results suggest that FGF2 can boost the proliferative capacity of damaged ovarian granulosa cells through the Hippo-YAP signaling pathway, providing a theoretical foundation for using EnSCs and FGF2 in clinical treatments for POF.

接受抗癌治疗的年轻女性面临卵巢早衰(POF)的风险。子宫内膜干细胞(EnSCs)在治疗卵巢功能不全方面具有显著的治疗潜力,但其潜在机制仍有待充分了解。本研究旨在进一步探讨EnSCs的治疗效果,特别是通过成纤维细胞生长因子2(FGF2)的旁分泌作用对卵巢功能不全的治疗效果。研究结果表明,外源性FGF2可提高受顺铂损伤的卵巢颗粒细胞的存活率。FGF2通过抑制Hippo信号通路和激活YAP的表达,刺激这些受损细胞的增殖。体内实验还发现,FGF2 治疗可显著改善 POF 小鼠的卵巢储备和内分泌功能。这些结果表明,FGF2可通过Hippo-YAP信号通路促进受损卵巢颗粒细胞的增殖能力,为将EnSCs和FGF2用于POF的临床治疗提供了理论基础。
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引用次数: 0
期刊
Molecular and Cellular Endocrinology
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