Pub Date : 2026-02-27DOI: 10.1016/j.mocell.2026.100341
Jaewon Ko
{"title":"A decade of discovery: Deciphering the synaptic adhesion code.","authors":"Jaewon Ko","doi":"10.1016/j.mocell.2026.100341","DOIUrl":"10.1016/j.mocell.2026.100341","url":null,"abstract":"","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":" ","pages":"100341"},"PeriodicalIF":6.5,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147326733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-23DOI: 10.1016/j.mocell.2026.100334
Hannah Lee, Ji Soo Chae, Hyun Woo Park
Bioluminescence imaging (BLI) using luciferase-expressing cancer cells is a widely adopted method for non-invasive, real-time monitoring of tumor growth, metastasis, and therapeutic responses in vivo. Here, we summarize key considerations for effective implementation of BLI, including luciferin administration and data quantification. We also address common technical challenges and provide practical troubleshooting strategies to support the use of BLI in preclinical tumor models.
{"title":"A practical guideline for in vivo bioluminescence imaging.","authors":"Hannah Lee, Ji Soo Chae, Hyun Woo Park","doi":"10.1016/j.mocell.2026.100334","DOIUrl":"https://doi.org/10.1016/j.mocell.2026.100334","url":null,"abstract":"<p><p>Bioluminescence imaging (BLI) using luciferase-expressing cancer cells is a widely adopted method for non-invasive, real-time monitoring of tumor growth, metastasis, and therapeutic responses in vivo. Here, we summarize key considerations for effective implementation of BLI, including luciferin administration and data quantification. We also address common technical challenges and provide practical troubleshooting strategies to support the use of BLI in preclinical tumor models.</p>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":" ","pages":"100334"},"PeriodicalIF":6.5,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147308331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-01Epub Date: 2026-01-29DOI: 10.1016/S1016-8478(26)00015-4
{"title":"Cover and caption","authors":"","doi":"10.1016/S1016-8478(26)00015-4","DOIUrl":"10.1016/S1016-8478(26)00015-4","url":null,"abstract":"","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"49 2","pages":"Article 100324"},"PeriodicalIF":6.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146090274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-01Epub Date: 2026-01-04DOI: 10.1016/j.mocell.2026.100311
Yikhyeon Seo , Jimin Pak , Jiyun Han , Joonbeom Bae , Soo Seok Hwang
Viral transduction of primary T cells enables stable genetic engineering for research and immunotherapy, supporting both transgene overexpression and gene deletion. Although the overall workflow can be similar to transduction in other mammalian cell lines, primary T cell culture imposes distinct requirements such as cell-state-dependent nuances shaped by T cell activation and proliferation, which can make it challenging to obtain a sufficient number of genetically engineered T cells. This article provides practical guidance for researchers new to T cells but familiar with basic mammalian cell culture.
{"title":"Viral transduction for T cell engineering in immunotherapy","authors":"Yikhyeon Seo , Jimin Pak , Jiyun Han , Joonbeom Bae , Soo Seok Hwang","doi":"10.1016/j.mocell.2026.100311","DOIUrl":"10.1016/j.mocell.2026.100311","url":null,"abstract":"<div><div>Viral transduction of primary T cells enables stable genetic engineering for research and immunotherapy, supporting both transgene overexpression and gene deletion. Although the overall workflow can be similar to transduction in other mammalian cell lines, primary T cell culture imposes distinct requirements such as cell-state-dependent nuances shaped by T cell activation and proliferation, which can make it challenging to obtain a sufficient number of genetically engineered T cells. This article provides practical guidance for researchers new to T cells but familiar with basic mammalian cell culture.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"49 2","pages":"Article 100311"},"PeriodicalIF":6.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145912427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-01Epub Date: 2025-12-29DOI: 10.1016/j.mocell.2025.100309
Yujing Wang , Zhixiang Le , Rujie Shi , Kun Li
Fibrosis is a chronic, progressive disease characterized by the excessive accumulation of extracellular matrix (ECM) in tissues and organs during damage-repair responses. This pathological process can involve almost any tissue or organ and may eventually lead to organ failure, posing a major threat to human health. ECM production is closely related to intercellular communication. As one of the biologically active substances participating in intercellular communication, exosomes have attracted increasing attention in recent years. In particular, noncoding RNAs (ncRNAs) enriched in exosomes regulate gene expression at multiple levels and influence the fibrosis process. Common ncRNAs include miRNA, long ncRNAs, circRNA, and tRNA, which can be selectively loaded into exosomes by various cells to modulate receptor cell functions. In fibrosis-related diseases, the primary sources of exosome-derived ncRNAs (Exo-ncRNAs) include mesenchymal stem cells, macrophages, epithelial cells, and fibroblasts. These Exo-ncRNAs regulate macrophage polarization, epithelial-mesenchymal transition, and fibroblast-myofibroblast transdifferentiation within the microenvironment. In this review, we summarize the regulatory roles and molecular mechanisms of these ncRNAs in the fibrosis process, and discuss Exo-ncRNAs with potential therapeutic effects. Understanding Exo-ncRNAs from different cell sources may provide new research directions for pathological intervention and the treatment of multiorgan fibrosis.
{"title":"Roles of Exosome–Derived Noncoding RNA in Fibrosis","authors":"Yujing Wang , Zhixiang Le , Rujie Shi , Kun Li","doi":"10.1016/j.mocell.2025.100309","DOIUrl":"10.1016/j.mocell.2025.100309","url":null,"abstract":"<div><div>Fibrosis is a chronic, progressive disease characterized by the excessive accumulation of extracellular matrix (ECM) in tissues and organs during damage-repair responses. This pathological process can involve almost any tissue or organ and may eventually lead to organ failure, posing a major threat to human health. ECM production is closely related to intercellular communication. As one of the biologically active substances participating in intercellular communication, exosomes have attracted increasing attention in recent years. In particular, noncoding RNAs (ncRNAs) enriched in exosomes regulate gene expression at multiple levels and influence the fibrosis process. Common ncRNAs include miRNA, long ncRNAs, circRNA, and tRNA, which can be selectively loaded into exosomes by various cells to modulate receptor cell functions. In fibrosis-related diseases, the primary sources of exosome-derived ncRNAs (Exo-ncRNAs) include mesenchymal stem cells, macrophages, epithelial cells, and fibroblasts. These Exo-ncRNAs regulate macrophage polarization, epithelial-mesenchymal transition, and fibroblast-myofibroblast transdifferentiation within the microenvironment. In this review, we summarize the regulatory roles and molecular mechanisms of these ncRNAs in the fibrosis process, and discuss Exo-ncRNAs with potential therapeutic effects. Understanding Exo-ncRNAs from different cell sources may provide new research directions for pathological intervention and the treatment of multiorgan fibrosis.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"49 2","pages":"Article 100309"},"PeriodicalIF":6.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145878705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-01Epub Date: 2026-01-05DOI: 10.1016/j.mocell.2026.100310
Eun-Ji Lee , Jae-Geun Lee , Jeong-Soo Lee
In vivo cell ablation technologies are essential tools for understanding biological processes within living animal models. The nitroreductase (NTR)/metronidazole system enables highly effective spatiotemporal cell ablation. Using transgenic zebrafish that combine NTR2.0 with the QF3/QUAS binary gene expression system, conditions to achieve efficient cell type–specific chemogenetic ablation were optimized. This approach provides a versatile in vivo platform for investigating developmental processes and regeneration, as well as for disease modeling and drug discovery.
{"title":"A brief guide to the in vivo chemogenetic cell ablation approach in zebrafish","authors":"Eun-Ji Lee , Jae-Geun Lee , Jeong-Soo Lee","doi":"10.1016/j.mocell.2026.100310","DOIUrl":"10.1016/j.mocell.2026.100310","url":null,"abstract":"<div><div>In vivo cell ablation technologies are essential tools for understanding biological processes within living animal models. The nitroreductase (NTR)/metronidazole system enables highly effective spatiotemporal cell ablation. Using transgenic zebrafish that combine NTR2.0 with the QF3/QUAS binary gene expression system, conditions to achieve efficient cell type–specific chemogenetic ablation were optimized. This approach provides a versatile in vivo platform for investigating developmental processes and regeneration, as well as for disease modeling and drug discovery.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"49 2","pages":"Article 100310"},"PeriodicalIF":6.5,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145918034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-01Epub Date: 2025-12-11DOI: 10.1016/j.mocell.2025.100302
Jiwon Choi , Geert De Jaeger , Hoo Sun Chung
Cytokinesis, the final stage of cell division, physically partitions the cytoplasm between daughter cells through mechanisms evolved to accommodate unique cellular constraints. Plant cells divide by the formation of rigid cell walls using the phragmoplast—a specialized structure guiding centrifugal cell plate formation from the cell center outward. Despite structural differences from the animal contractile ring mechanism, plant and animal cytokinesis share fundamental similarities in division plane determination, vesicle trafficking, and conserved proteins, including kinesins and microtubule-associated proteins. This conservation alongside kingdom-specific adaptations makes plant cytokinesis an excellent model for understanding evolutionary divergence. Recent technological advances have enabled detailed characterization of molecular components and regulatory networks controlling spatiotemporal progression through post translational modifications. In this review, we provide an integrated perspective of plant cytokinesis, examining cellular dynamics from division plane determination to cell plate maturation, molecular machinery driving these processes, and kinase-mediated regulatory networks ensuring precise coordination of this complex process.
{"title":"Cellular dynamics and molecular signaling networks of plant cytokinesis","authors":"Jiwon Choi , Geert De Jaeger , Hoo Sun Chung","doi":"10.1016/j.mocell.2025.100302","DOIUrl":"10.1016/j.mocell.2025.100302","url":null,"abstract":"<div><div>Cytokinesis, the final stage of cell division, physically partitions the cytoplasm between daughter cells through mechanisms evolved to accommodate unique cellular constraints. Plant cells divide by the formation of rigid cell walls using the phragmoplast—a specialized structure guiding centrifugal cell plate formation from the cell center outward. Despite structural differences from the animal contractile ring mechanism, plant and animal cytokinesis share fundamental similarities in division plane determination, vesicle trafficking, and conserved proteins, including kinesins and microtubule-associated proteins. This conservation alongside kingdom-specific adaptations makes plant cytokinesis an excellent model for understanding evolutionary divergence. Recent technological advances have enabled detailed characterization of molecular components and regulatory networks controlling spatiotemporal progression through post translational modifications. In this review, we provide an integrated perspective of plant cytokinesis, examining cellular dynamics from division plane determination to cell plate maturation, molecular machinery driving these processes, and kinase-mediated regulatory networks ensuring precise coordination of this complex process.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"49 1","pages":"Article 100302"},"PeriodicalIF":6.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145743384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-01Epub Date: 2025-11-26DOI: 10.1016/j.mocell.2025.100301
Seok Chan Cho, Hyun Woo Park
Protein-protein interaction (PPI) is central to all cellular processes and play critical roles in both normal physiology and disease pathogenesis. In this brief guide, we outline the fundamental principles and widely used methods for PPI detection, focusing on 3 key techniques: immunoprecipitation, in vitro pull-down assays, and proximity ligation assay. We also discuss common experimental challenges and provide practical optimization strategies to improve reliability and reproducibility. This resource is designed to aid researchers in molecular and cellular biology, signal transduction, and animal model studies with essential knowledge for selecting and applying PPI detection methods.
{"title":"Methods for detecting protein-protein interactions","authors":"Seok Chan Cho, Hyun Woo Park","doi":"10.1016/j.mocell.2025.100301","DOIUrl":"10.1016/j.mocell.2025.100301","url":null,"abstract":"<div><div>Protein-protein interaction (PPI) is central to all cellular processes and play critical roles in both normal physiology and disease pathogenesis. In this brief guide, we outline the fundamental principles and widely used methods for PPI detection, focusing on 3 key techniques: immunoprecipitation, in vitro pull-down assays, and proximity ligation assay. We also discuss common experimental challenges and provide practical optimization strategies to improve reliability and reproducibility. This resource is designed to aid researchers in molecular and cellular biology, signal transduction, and animal model studies with essential knowledge for selecting and applying PPI detection methods.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"49 1","pages":"Article 100301"},"PeriodicalIF":6.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145636128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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