Nauchnye doklady vysshei shkoly. Biologicheskie nauki最新文献

The data on the completing level of a number of medical preparations on the basis of immobilized proteins, in particular, on the basis of enzymes are given. The firms and companies which successfully cooperate in the field of development of commercial programs for such preparations are listed. On the basis of own study a possibility to create some effective antitumor compounds in the next future is shown. The urgent and prospective application of the immobilized enzymes instead of the native ones in the medicine is grounded.

The methodological approaches of isolation of preparations of FMDV structural polypeptides to analyse them by the electrophoresis and electro-focussing methods are presented. The value of isoelectric points of protein coat of FMDV structural polypeptides and corresponding them values of electric potential are determined. The similarity and differences of FMDV serotypes, characterized by the value of relative surface, falling on separate polypeptides, are determined for the virion structure on the basis of superposition principle. FMDV has been shown to possess the summarized negative charge of different values. The charge depends on the virus type and it is a determining condition for viruses resistance in environment. A graphical model of FMDV is suggested on the basis of systemic approach and it reflects the dipole character of electric charge distribution in virion structure and agrees with the virus model, built on the basis of icosahedron symmetry.

Social-economic remakings in Siberia are reflected in the economy of a rural inhabitant and the sex-age structure of the Khakass populations. The age average of the population increased from 26.7 years in 1954 up to 31.2 years in 1980. It has been established that the effectively reproductive size of a population (Ne) which takes into account its historical extent serves as a satisfactory estimate of the veritable (not single, instantaneous) size of a population.

The effect of Bacillus intermedius RNAse on the reproduction of Candida tropicalis and synthesis of the main biopolymers in the yeast cells. It has been found that stimulating action of the enzyme appears at the concentration of 10(-5)-10(-6) mg/ml and does not depend on the physiological state of the sowing culture. The connection between the increase of the ionic penetration and stimulation of the RNA and proteins synthesis in the yeast cells subjected to the RNAse action is shown. The mechanism of chromatine-associated RNA-polymerase activation is suggested to include the alteration of the ionic penetration of cells under the RNAse action.

The biological methods of animal organism protection, the role of stress and the nociceptive signals, the hierarchy of defence mechanism--from the autonomic reflexes, neuroendocrine regulations, emotional reactions and expressions up to peculiar to the human being intellectual safeguard have been considered. The latest data on the role of tachykinins in transmission have been examined. The discovery of mediator function of "substance P" (SP), other related peptides, which coexists and realizes from the primary afferents and other terminals of SP-containing, capsaicin-sensitive neurons--opened a new chapter in the neurobiology and medicine. The necessity to support the medico-biological (fundamental) experiments by the modern society is grounded.

The effect of immunostimulator--Freund's adjuvant complete on the nociception and learning of white rats using food-obtaining and avoidance of electric shock techniques has been studied. It has been shown that the adjuvant in doses for active immunization causes itself the expressed changes in animal behaviour. The adjuvant's injections significantly increases the learning ability of animals both with negative and positive reinforcement as compared with control one. The changes in pain severity is marked only for dose 0.2 ml. The nonspecific action of adjuvant should be taken into account in researches which use the active immunisation method.

The preparation Hypericum perforatum L. has been shown to decrease the intensity level of enzymatic and non-enzymatic processes of lipid peroxidation of rat liver microsomes in vitro and in vivo. In the mucus of the this intestine in vivo the increase of the number of non-enzymatic SH-groups is observed. Possible mechanisms of radioprotective action of the preparation is under discussion.

Lactase polymorphism was studied in the native population of West Siberia and also in Buryatia. LAC*R frequency observed is-Khants- 0.8367, Mansi - 0.8660, Nenets - 0.8944, Buryats - 0.6883. The data obtained are considered to be the result of natural selection under traditional historical economical-cultural environment of the ethnic groups in question.

In order to obtain nuclease and human serum albumin (HSA) conjugates with a high enzyme content it is proposed to use a ligand-free HSA. The ligands are removed with the help of a strong anion exchanger. A two-stage procedure of conjugate preparation is proposed. It consists in the complexation of ligand-free HSA and enzyme and subsequent co-condensation of protein molecules of the poly-complex with the aid of glutaric aldehyde. When the conjugates are administered to rabbits intravenously, the RNAase activity is manifested in blood for 3-5 days. Moreover, in the case of conjugates with a molecular weight of 80 kDa, the prolongation time is greater than for conjugate with a higher molecular weight.

It is shown that the action of ultraviolet rays (UVR) or magnetic field (MF) on the erythrocytes of intact Wistar rats by weight 140-170 g induces the property to stimulate the immune response on sheep's T-dependent antigen-erythrocytes and on bull serum albumin in allogenic transference. The most expressed immune stimulated effect is induced by the heavy erythrocytes, which are effected by magnetic field. The warming and vibration don't induce the immunomodulating characteristics in erythrocytes. By spleen cells sticking to glass, the modified UVR and MF, the heavy erythrocytes induce factors (differentiated in mass), which stimulate the development of immune response to T-dependent antigens of sheep's erythrocytes and of bull serum albumin. These factors depress the function of antigen-specific T-suppressors and induce the immune-suppressive characteristics in light erythrocytes.