Pub Date : 2023-06-01DOI: 10.26599/nbe.2023.9290020
Sonia Parashar, M. Garg
{"title":"Optimized Green Synthesis of \u0000 Manilkara zapota Capped Silver Nanoparticles and Their Antimicrobial Application Through Formulation of Nano-gel Systems","authors":"Sonia Parashar, M. Garg","doi":"10.26599/nbe.2023.9290020","DOIUrl":"https://doi.org/10.26599/nbe.2023.9290020","url":null,"abstract":"","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45737734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-01DOI: 10.26599/nbe.2023.9290016
M. Hassan, A. H. Mohammed, Ekhlas Majeed Hameed
{"title":"Application of \u0000 Aloe vera Gel Blended Polymer-Collagen Scaffolds for Bone Tissue Engineering","authors":"M. Hassan, A. H. Mohammed, Ekhlas Majeed Hameed","doi":"10.26599/nbe.2023.9290016","DOIUrl":"https://doi.org/10.26599/nbe.2023.9290016","url":null,"abstract":"","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46415168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.26599/nbe.2023.9290014
Bairaboina Sai Samba SivaRao, B. S. Rao
In the human body, white blood cells (WBCs) are crucial immune cells that help in the early detection of a variety of illnesses. Determination of the number of WBCs can be used to diagnose conditions such as hematological, immunological, and autoimmune diseases, as well as AIDS and leukemia. However, the conventional method of classifying and counting WBCs is time-consuming, laborious, and potentially erroneous. Therefore, this paper presents a computer-assisted automated method for recognizing and detecting WBC categories from blood images. Initially, the blood cell image is preprocessed and then segmented using an effective deep learning architecture called SegNet. Then, the important features are devised and extracted using the EfficientNet architecture. Finally, the WBCs are categorized into four different types using the XGBoost classifier: neutrophils, eosinophils, monocytes, and lymphocytes. The advantages of SegNet, EfficientNet, and XGBoost make the proposed model more robust and achieve a more efficient classification of the WBCs. The BCCD dataset is used to evaluate the performance of the proposed methodology, and the findings are compared to existing state-of-the-art approaches based on accuracy, precision, sensitivity, specificity, and F1-score. Evaluation results show that the proposed approach has a higher rank-1 accuracy of 99.02% and outperformed other existing techniques.
{"title":"EfficientNet - XGBoost: An Effective White-Blood-Cell Segmentation and Classification Framework","authors":"Bairaboina Sai Samba SivaRao, B. S. Rao","doi":"10.26599/nbe.2023.9290014","DOIUrl":"https://doi.org/10.26599/nbe.2023.9290014","url":null,"abstract":"In the human body, white blood cells (WBCs) are crucial immune cells that help in the early detection of a variety of illnesses. Determination of the number of WBCs can be used to diagnose conditions such as hematological, immunological, and autoimmune diseases, as well as AIDS and leukemia. However, the conventional method of classifying and counting WBCs is time-consuming, laborious, and potentially erroneous. Therefore, this paper presents a computer-assisted automated method for recognizing and detecting WBC categories from blood images. Initially, the blood cell image is preprocessed and then segmented using an effective deep learning architecture called SegNet. Then, the important features are devised and extracted using the EfficientNet architecture. Finally, the WBCs are categorized into four different types using the XGBoost classifier: neutrophils, eosinophils, monocytes, and lymphocytes. The advantages of SegNet, EfficientNet, and XGBoost make the proposed model more robust and achieve a more efficient classification of the WBCs. The BCCD dataset is used to evaluate the performance of the proposed methodology, and the findings are compared to existing state-of-the-art approaches based on accuracy, precision, sensitivity, specificity, and F1-score. Evaluation results show that the proposed approach has a higher rank-1 accuracy of 99.02% and outperformed other existing techniques.","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47604991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.26599/nbe.2023.9290012
Hema Gunti, Susmila Aparna Gaddam, Ramamurthy Nadipi, V. Kotakadi
This study was conducted to identify promising applications of green silver nanoparticles (AgNPs) prepared from a bark extract of Sweetinia mahagoni (Sm). The green synthesized Sm-AgNPs were characterized using various spectroscopy methods. AgNPs were first investigated using ultraviolet–visible spectroscopy, and the metal nanoparticles exhibited an intense surfaceplasmon resonance (SPR) peak at different wavelengths. The green synthesized Sm-AgNPs had an SPR peak at 430 nm, which confirms the formation of Sm-AgNPs. In addition, Fourier transform infrared (FTIR) spectroscopy was conducted to determine the bioactive compounds of bark extract that actively participate in the reduction of Sm-AgNPs, and the results revealed O−H stretching of free hydroxyl alcohol and phenols, N−H bonds of primary amines, S ＝ O stretching of sulfoxide in aromatic groups, C−I stretching due to aliphatic iodo compounds, and C−Br stretching by halo compounds of the bark extract which might reduce and stabilize Sm-AgNPs. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) results revealed that Sm-AgNPs were approximately irregular spheres. EDS results revealed the complete reduction of silver to elemental silver. The particle size analysis of Sm-AgNPs was conducted using dynamic light scattering (DLS), and the results revealed that Sm-AgNPs were polydisperse with an average size range from 35.8 to 47.8 nm, an average mean size of 41.3 nm, and a Z average of 37.7 nm. Sm-AgNPs had a negative zeta potential value of −19.0 mV, indicating that Sm-AgNPs were very stable in colloidal form. Further studies were carried out to demonstrate their usefulness in industrial and biomedical applications. In these studies, Sm-AgNPs exhibited a very good antibacterial activity against both Gram-negative and Gram-positive bacteria. In addition to regular assays, we also investigated important industrial applications such as the reduction of toxic hexavalent chromium to a nontoxic form and sensing of Hg 2+ ions. The results revealed that Sm-AgNPs had an excellent performance in biosensor applications such as sensing and detecting mercury at parts per million/parts per billion levels. In conclusion, green Sm-AgNPs are promising materials in therapeutic and industrial applications.
{"title":"Optical and Paper-based Dual Sensing of Hg\u0000 2+ and Colorimetric Reduction of Cr(VI) by Green Synthesized Silver Nanoparticles Prepared from the Bark Extract of \u0000 Sweetinia mahagoni and Their Promising Antimicrobial Applications","authors":"Hema Gunti, Susmila Aparna Gaddam, Ramamurthy Nadipi, V. Kotakadi","doi":"10.26599/nbe.2023.9290012","DOIUrl":"https://doi.org/10.26599/nbe.2023.9290012","url":null,"abstract":"This study was conducted to identify promising applications of green silver nanoparticles (AgNPs) prepared from a bark extract of Sweetinia mahagoni (Sm). The green synthesized Sm-AgNPs were characterized using various spectroscopy methods. AgNPs were first investigated using ultraviolet–visible spectroscopy, and the metal nanoparticles exhibited an intense surfaceplasmon resonance (SPR) peak at different wavelengths. The green synthesized Sm-AgNPs had an SPR peak at 430 nm, which confirms the formation of Sm-AgNPs. In addition, Fourier transform infrared (FTIR) spectroscopy was conducted to determine the bioactive compounds of bark extract that actively participate in the reduction of Sm-AgNPs, and the results revealed O−H stretching of free hydroxyl alcohol and phenols, N−H bonds of primary amines, S ＝ O stretching of sulfoxide in aromatic groups, C−I stretching due to aliphatic iodo compounds, and C−Br stretching by halo compounds of the bark extract which might reduce and stabilize Sm-AgNPs. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) results revealed that Sm-AgNPs were approximately irregular spheres. EDS results revealed the complete reduction of silver to elemental silver. The particle size analysis of Sm-AgNPs was conducted using dynamic light scattering (DLS), and the results revealed that Sm-AgNPs were polydisperse with an average size range from 35.8 to 47.8 nm, an average mean size of 41.3 nm, and a Z average of 37.7 nm. Sm-AgNPs had a negative zeta potential value of −19.0 mV, indicating that Sm-AgNPs were very stable in colloidal form. Further studies were carried out to demonstrate their usefulness in industrial and biomedical applications. In these studies, Sm-AgNPs exhibited a very good antibacterial activity against both Gram-negative and Gram-positive bacteria. In addition to regular assays, we also investigated important industrial applications such as the reduction of toxic hexavalent chromium to a nontoxic form and sensing of Hg 2+ ions. The results revealed that Sm-AgNPs had an excellent performance in biosensor applications such as sensing and detecting mercury at parts per million/parts per billion levels. In conclusion, green Sm-AgNPs are promising materials in therapeutic and industrial applications.","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43849641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.26599/nbe.2023.9290009
Wichai Subtaweewasin, W. Pijitrojana
Kidney disease, as a global health problem, can progress to kidney failure. Kidney transplantation is a treatment option for end-stage disease and an alternative to dialysis. Complement-dependent cytotoxicity tests and flow cytometry are methods used to test for compatibility between autoantibodies and donor recipient. Antibodies against donor human leukocyte antigens (HLAs), linked to hyperacute or severely acute rejection, frequently result in graft loss. Currently, fluorescent bead assay (Luminex ® ) technology is used in Thailand for HLA–antibody typing tests. However, each test requires specific location and time, expensive equipment, and high costs. The goal of such research is that, HLA–antibody typing tests can be performed wherever it is the most convenient for recipients and hospitals. A further advantage is that the surgeon can perform a final test for confirmation before the surgery. Numerous technologies are currently available for this purpose. The focus of this study was on the Fe 3 O 4 magnetic nanoparticles (MNPs) immobilized with HLAs frequently found in the Thai population. These were examined using nuclear magnetic resonance (NMR) to measure HLA antibody typing. MNPs have recently attracted considerable attention because of their strong magnetization and large surface areas. Immobilization reactions with antigens and the straightforward magnetic separation of MNPs are low-cost techniques. Hence, the commercialization of Fe 3 O 4 MNPs is favorable. In this study, MNPs were functionalized with 3-aminopropyl triethoxysilane (APTES) in toluene and ethanol, and the nanoparticles were streptavidin activated after APTES treatment. A specific biotinylated HLA for the Thai population was used and immobilized on streptavidin. The MNPs were effectively immobilized. The magnetic properties can be modified by adding other ions to Fe 3 O 4 . We discovered that the shape and size variations of Fe 3 O 4 MNPs may have an impact on the amount of functionalized and immobilized surface area.
肾脏疾病作为一个全球性的健康问题，可以发展为肾衰竭。肾移植是治疗终末期疾病的一种选择，也是透析的一种替代方案。补体依赖性细胞毒性测试和流式细胞术是用于测试自身抗体和供体受体之间兼容性的方法。针对供体人类白细胞抗原（HLAs）的抗体，与超急性或严重急性排斥反应有关，经常导致移植物丢失。目前，荧光珠分析（Luminex®）技术在泰国用于HLA抗体分型测试。然而，每次测试都需要特定的位置和时间、昂贵的设备和高昂的成本。这项研究的目标是，HLA抗体分型测试可以在对接受者和医院最方便的地方进行。另一个优点是外科医生可以在手术前进行最终测试以进行确认。目前有许多技术可用于此目的。本研究的重点是用在泰国人群中常见的HLAs固定化的Fe3O4磁性纳米颗粒（MNPs）。使用核磁共振（NMR）来检测HLA抗体分型。MNP由于其强磁化和大表面积，最近引起了相当大的关注。与抗原的固定化反应和MNP的直接磁性分离是低成本的技术。因此，Fe3 O4 MNPs的商业化是有利的。在本研究中，用3-氨基丙基三乙氧基硅烷（APTES）在甲苯和乙醇中对MNPs进行了功能化，并在APTES处理后对纳米粒子进行了链亲和素活化。使用了一种用于泰国人群的特异性生物素化HLA，并将其固定在链霉亲和素上。MNP被有效固定。在Fe3O4中加入其它离子可以改变磁性能。我们发现，Fe3 O 4 MNPs的形状和尺寸变化可能会影响其功能化和固定化的表面积。
{"title":"Immobilization of Thai Population-specific Human Leukocyte Antigens on Magnetic Nanoparticles Integrated with Nuclear Magnetic Resonance Technology","authors":"Wichai Subtaweewasin, W. Pijitrojana","doi":"10.26599/nbe.2023.9290009","DOIUrl":"https://doi.org/10.26599/nbe.2023.9290009","url":null,"abstract":"Kidney disease, as a global health problem, can progress to kidney failure. Kidney transplantation is a treatment option for end-stage disease and an alternative to dialysis. Complement-dependent cytotoxicity tests and flow cytometry are methods used to test for compatibility between autoantibodies and donor recipient. Antibodies against donor human leukocyte antigens (HLAs), linked to hyperacute or severely acute rejection, frequently result in graft loss. Currently, fluorescent bead assay (Luminex ® ) technology is used in Thailand for HLA–antibody typing tests. However, each test requires specific location and time, expensive equipment, and high costs. The goal of such research is that, HLA–antibody typing tests can be performed wherever it is the most convenient for recipients and hospitals. A further advantage is that the surgeon can perform a final test for confirmation before the surgery. Numerous technologies are currently available for this purpose. The focus of this study was on the Fe 3 O 4 magnetic nanoparticles (MNPs) immobilized with HLAs frequently found in the Thai population. These were examined using nuclear magnetic resonance (NMR) to measure HLA antibody typing. MNPs have recently attracted considerable attention because of their strong magnetization and large surface areas. Immobilization reactions with antigens and the straightforward magnetic separation of MNPs are low-cost techniques. Hence, the commercialization of Fe 3 O 4 MNPs is favorable. In this study, MNPs were functionalized with 3-aminopropyl triethoxysilane (APTES) in toluene and ethanol, and the nanoparticles were streptavidin activated after APTES treatment. A specific biotinylated HLA for the Thai population was used and immobilized on streptavidin. The MNPs were effectively immobilized. The magnetic properties can be modified by adding other ions to Fe 3 O 4 . We discovered that the shape and size variations of Fe 3 O 4 MNPs may have an impact on the amount of functionalized and immobilized surface area.","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47184081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.26599/nbe.2023.9290015
M. Khalaf, M. Hassan, A. H. Mohammed
{"title":"Exploring the Role of Phytochemicals: Effect of [6]-Gingerol Combined with Colloidal Gold Nanoparticles on Thyroid Carcinoma Cells","authors":"M. Khalaf, M. Hassan, A. H. Mohammed","doi":"10.26599/nbe.2023.9290015","DOIUrl":"https://doi.org/10.26599/nbe.2023.9290015","url":null,"abstract":"","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45729004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.26599/nbe.2023.9290011
R. Chavan, M. Bhutkar, S. Bhinge
In this study, the synthesis of nanoparticles and their biological evaluation were carried out. A green synthetic approach synthesized silver nanoparticles (AHAgNPs) using an Artocarpus heterophyllus leaf extract. Parameter optimization was performed using Design Expert Ver. 13. The effects of variables like the concentration on the response, particle size, and entrapment efficiency of synthesized AHAgNPs were monitored via analysis of variance. The optimized AgNPs were characterized using ultraviolet–visible spectroscopy and Fourier transform infrared spectroscopy. Scanning electron microscopy and transmission electron microscopy were used to determine the size and shape of nanoparticles. In vitro, antioxidant and antimicrobial potential were determined using standard protocols. The optimized nanoparticles were spherical, with an average 100–110 nm particle diameter. The synthesized nanoparticles showed effective antioxidant, antibacterial, and antifungal activity. In addition, AHAgNPs showed increased biological activities.
{"title":"Design, Synthesis, and Optimization of Silver Nanoparticles Using an \u0000 Artocarpus heterophyllus Lam. Leaf Extract and Its Antibacterial Application","authors":"R. Chavan, M. Bhutkar, S. Bhinge","doi":"10.26599/nbe.2023.9290011","DOIUrl":"https://doi.org/10.26599/nbe.2023.9290011","url":null,"abstract":"In this study, the synthesis of nanoparticles and their biological evaluation were carried out. A green synthetic approach synthesized silver nanoparticles (AHAgNPs) using an Artocarpus heterophyllus leaf extract. Parameter optimization was performed using Design Expert Ver. 13. The effects of variables like the concentration on the response, particle size, and entrapment efficiency of synthesized AHAgNPs were monitored via analysis of variance. The optimized AgNPs were characterized using ultraviolet–visible spectroscopy and Fourier transform infrared spectroscopy. Scanning electron microscopy and transmission electron microscopy were used to determine the size and shape of nanoparticles. In vitro, antioxidant and antimicrobial potential were determined using standard protocols. The optimized nanoparticles were spherical, with an average 100–110 nm particle diameter. The synthesized nanoparticles showed effective antioxidant, antibacterial, and antifungal activity. In addition, AHAgNPs showed increased biological activities.","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44039921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.26599/nbe.2023.9290008
Rajath Othayoth, Karishma Khatri, Ramya Gadicherla, Suseela Kodandapani, M. Botlagunta
Vitamin supplementation during chemotherapy has often been associated with lower recurrence and mortality rates in cancer patients. We had previously demonstrated that the multivitamin (C, D3, and B12)–cisplatin nanoparticle complex—NanoCisVital (NCV)— could alleviate chemotherapy-induced cancer fatigue. Chitosan is frequently used in functional nanomaterials to encapsulate drugs, because it is biodegradable, biocompatible, and non-toxic. The chitosan-based NCVs were prepared, and their physicochemical properties, size, and stability were evaluated before assessing their effect on cancer cell lines. The multivitamin mixture is packed in the core, and cisplatin is loaded at the periphery of the nanoparticle. This encapsulation facilitates the slow and sequential release of peripheral cisplatin and the core multivitamin combination. By increasing the amounts of vitamin and drug-encapsulated nanoparticles in breast and cervical cancer cell lines, the viable cell percentage was calculated. DDX3X promotes cancer cell proliferation, invasion, and metastasis, while Ki-67 promotes active cell proliferation in all cell types. DDX3X is elevated in several cancer types, and breast cancer cells express it abnormally. The Ki-67 protein is a biomarker of cell proliferation that is present throughout all active stages of the cell cycle but undetectable in the resting state. The expression of the DDX3X and Ki-67 genes is altered in NCV-treated cells. This study uses DDX3X and Ki-67 gene expression as a comparative measuring tool for the anti-cancer and cell proliferation effects of cisplatin and vitamins, respectively.
{"title":"Multivitamin–Cisplatin Encapsulated Chitosan Nanoparticles Modulate DDX3X Expression in Cancer Cell Lines","authors":"Rajath Othayoth, Karishma Khatri, Ramya Gadicherla, Suseela Kodandapani, M. Botlagunta","doi":"10.26599/nbe.2023.9290008","DOIUrl":"https://doi.org/10.26599/nbe.2023.9290008","url":null,"abstract":"Vitamin supplementation during chemotherapy has often been associated with lower recurrence and mortality rates in cancer patients. We had previously demonstrated that the multivitamin (C, D3, and B12)–cisplatin nanoparticle complex—NanoCisVital (NCV)— could alleviate chemotherapy-induced cancer fatigue. Chitosan is frequently used in functional nanomaterials to encapsulate drugs, because it is biodegradable, biocompatible, and non-toxic. The chitosan-based NCVs were prepared, and their physicochemical properties, size, and stability were evaluated before assessing their effect on cancer cell lines. The multivitamin mixture is packed in the core, and cisplatin is loaded at the periphery of the nanoparticle. This encapsulation facilitates the slow and sequential release of peripheral cisplatin and the core multivitamin combination. By increasing the amounts of vitamin and drug-encapsulated nanoparticles in breast and cervical cancer cell lines, the viable cell percentage was calculated. DDX3X promotes cancer cell proliferation, invasion, and metastasis, while Ki-67 promotes active cell proliferation in all cell types. DDX3X is elevated in several cancer types, and breast cancer cells express it abnormally. The Ki-67 protein is a biomarker of cell proliferation that is present throughout all active stages of the cell cycle but undetectable in the resting state. The expression of the DDX3X and Ki-67 genes is altered in NCV-treated cells. This study uses DDX3X and Ki-67 gene expression as a comparative measuring tool for the anti-cancer and cell proliferation effects of cisplatin and vitamins, respectively.","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46388916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.26599/nbe.2023.9290013
Haider Hamza Al-Shreefy, E. Al-Wasiti, Mohammed J Al-Awady
As cancer-related deaths continue to rise, developments in nanotechnology have emerged as a feasible option for finding successful treatments targeting cancerous cells while avoiding all of the drawbacks of traditional drugs. Selenium nanoparticles (SeNPs) have been reported to exhibit an inhibitory effect on cancerous cells. The aim of the present study was to use the drug delivery systems poly(lactic-co-glycolic acid) (PLGA) and poly(lactic-co-glycolic acid-poly(ethylene glycol)-folic acid (PLGA-PEG-FA) to encapsulate SeNPs and investigate their antineoplastic effects against two cell line types (MCF-7 as a positive folate receptor and HBL as a negative folate receptor) by exploiting overexpression features in some types of cancer cells to ensure delivery of drug molecules at high dosages toward targeted cells and circumvent normal cells/tissues. SeNPs were chemically synthesized and characterized with dynamic light scattering (DLS) and transmission electronic microscopy (TEM). The cytotoxicity of both nanomaterials was evaluated against MCF-7 and HBL cells by using the methyl thiazolyl tetrazolium (MTT) assay which showed a high cytotoxic effect against MCF-7 cells with a lesser effect against HBL cells. Additionally, an apoptosis assay was also performed by using acridine orange/ethidium bromide dual staining, and the antioxidant effect was also investigated by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) colorimetric method with high antioxidant potential for both formulations. They showed nonhemolytic activity on human red blood cells. This work could be considered promising for pharmaceutical formulation.
{"title":"Investigation of Encapsulated Selenium Nanoparticles with PLGA Polymers Against MCF-7 and HBL Cell Lines","authors":"Haider Hamza Al-Shreefy, E. Al-Wasiti, Mohammed J Al-Awady","doi":"10.26599/nbe.2023.9290013","DOIUrl":"https://doi.org/10.26599/nbe.2023.9290013","url":null,"abstract":"As cancer-related deaths continue to rise, developments in nanotechnology have emerged as a feasible option for finding successful treatments targeting cancerous cells while avoiding all of the drawbacks of traditional drugs. Selenium nanoparticles (SeNPs) have been reported to exhibit an inhibitory effect on cancerous cells. The aim of the present study was to use the drug delivery systems poly(lactic-co-glycolic acid) (PLGA) and poly(lactic-co-glycolic acid-poly(ethylene glycol)-folic acid (PLGA-PEG-FA) to encapsulate SeNPs and investigate their antineoplastic effects against two cell line types (MCF-7 as a positive folate receptor and HBL as a negative folate receptor) by exploiting overexpression features in some types of cancer cells to ensure delivery of drug molecules at high dosages toward targeted cells and circumvent normal cells/tissues. SeNPs were chemically synthesized and characterized with dynamic light scattering (DLS) and transmission electronic microscopy (TEM). The cytotoxicity of both nanomaterials was evaluated against MCF-7 and HBL cells by using the methyl thiazolyl tetrazolium (MTT) assay which showed a high cytotoxic effect against MCF-7 cells with a lesser effect against HBL cells. Additionally, an apoptosis assay was also performed by using acridine orange/ethidium bromide dual staining, and the antioxidant effect was also investigated by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) colorimetric method with high antioxidant potential for both formulations. They showed nonhemolytic activity on human red blood cells. This work could be considered promising for pharmaceutical formulation.","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48154550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.26599/nbe.2023.9290010
E. Sweedan, S. A. Abdul Majeed
,
，
{"title":"Effects of Silver Nanoparticles Synthesized from Phenolic Extract of \u0000 Agaricus bisporus Against Pathogenic Bacteria and Yeasts","authors":"E. Sweedan, S. A. Abdul Majeed","doi":"10.26599/nbe.2023.9290010","DOIUrl":"https://doi.org/10.26599/nbe.2023.9290010","url":null,"abstract":",","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44764208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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