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An Enhancing Diabetic Retinopathy Classification and Segmentation based on TaNet 基于 TaNet 的增强型糖尿病视网膜病变分类和分割技术
Q3 Engineering Pub Date : 2023-10-01 DOI: 10.26599/nbe.2023.9290041
Koneru Suvarna Vani, Puppala Praneeth, Vivek Kommareddy, Parasa Rishi Kumar, Madala Sarath, Shaik Hussain, Potluri Ravikiran
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引用次数: 0
The Impact of Modified Metallic Nanoparticles on Thermomechanical Properties of PMMA Soft Liner 改性金属纳米颗粒对PMMA软衬热力学性能的影响
Q3 Engineering Pub Date : 2023-10-01 DOI: 10.26599/nbe.2023.9290040
Ban Saad Jasim, Hasanain K.A. Alalwan, Abdalbseet A. Fatalla, Manar E. Al-Samaray
The acrylic-based heat-cured soft denture lining material is the most commonly used in relining dentures. This material has poor thermomechanical properties which is a disadvantage. This research aimed to study the effect of the addition of modified metallic nanoparticles on glass transition, modulus of elasticity, and coefficient of thermal expansion and contraction of the acrylic soft liner. Alumina nanoparticles were first modified by a silane coupling agent and then added to a soft denture liner powder in different weight percentages (0, 0.5, 1, and 1.5 wt%) using a probe ultrasonication machine for mixing. 120 samples of acrylic-based soft liner were constructed and divided into four groups G1–G4 (n = 30). Each group was in turn subdivided into 3 subgroups (n = 10) according to the test performed. The mean value, SD, Kruskal-Wallis test, and Dunn’s Multiple Comparison tests were used to analyze the results statistically. Incorporating 0.5% by weight alumina nano-fillers into acrylic-based heat-cured soft denture lining material, increased the glass transition temperature significantly (p ≤ 0.01). Additionally, it significantly reduced the coefficient of thermal expansion and contraction, especially at 30 ºC, compared to the control group. The E-modulus was also reduced, especially at 50 ºC, compared to the control group. According to the reported results, the polymer nanocomposites possess distinctive material properties that distinguish them from unmodified acrylic-base soft denture lining materials. Nanocomposites have more thermal and mechanical stability than unmodified acrylic-base soft denture lining material especially when incorporating 0.5 wt% Al2O3.
丙烯酸基热固化软性义齿衬里材料是义齿修复中最常用的衬里材料。这种材料的热机械性能很差,这是一个缺点。本研究旨在研究改性金属纳米颗粒的加入对丙烯酸软衬里玻璃化转变、弹性模量和热胀冷缩系数的影响。首先用硅烷偶联剂修饰氧化铝纳米颗粒,然后用探头超声机将不同重量百分比(0、0.5、1和1.5 wt%)的氧化铝纳米颗粒加入软义齿衬粉中进行混合。制作丙烯酸基软衬120个样品,分为G1-G4四组(n = 30)。每组根据所做的试验再细分为3个亚组(n = 10)。采用均数、标准差、Kruskal-Wallis检验和Dunn多重比较检验对结果进行统计学分析。在丙烯酸基热固化软基义齿衬里材料中加入0.5%重量氧化铝纳米填料,可显著提高玻璃化转变温度(p≤0.01)。此外,与对照组相比,它显著降低了热膨胀和收缩系数,特别是在30ºC时。与对照组相比,e模量也有所降低,特别是在50ºC时。研究结果表明,聚合物纳米复合材料具有不同于未改性丙烯酸基软基义齿衬里材料的独特材料性能。纳米复合材料比未改性的丙烯酸基软基义齿衬里材料具有更高的热稳定性和机械稳定性,特别是当添加0.5 wt% Al2O3时。
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引用次数: 0
Silver Nanoparticles Synthesized by Cold Plasma as an Antibiofilm Agent against Staphylococcus epidermidis Isolated from Acne 用冷等离子体合成的银纳米粒子作为抗生物膜剂对抗从痤疮中分离出来的表皮葡萄球菌
Q3 Engineering Pub Date : 2023-10-01 DOI: 10.26599/nbe.2023.9290042
Heba K. Tawfeeq, N. Abdalameer, R. Jassim, Maryam M. Shehab
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引用次数: 0
Nanoparticles-assisted Wound Healing: A Review 纳米颗粒辅助伤口愈合:综述
Q3 Engineering Pub Date : 2023-10-01 DOI: 10.26599/nbe.2023.9290039
Sarah Habeeb Hamed, Ebaa Adnan Azooz, Emad Abbas Jaffar Al-Mulla
Poor wound treatment impacts millions of humans worldwide, increasing deaths and costs. Wounds have three key complications: (a) a lack of an adequate environment for cell migration, proliferation, and angiogenesis; (b) microbial infection; and (c) unstable and prolonged inflammation.Regrettably, contemporary therapeutic treatments have not entirely tackled these basic difficulties and thus have insufficient medical accomplishment. The incorporation of the extraordinary capabilities of nanomaterials in wound healing has achieved major successes over the years. Nanomaterials can promote a variety of cellular and molecular processes that assist in the wound microenvironment through antibacterial, anti-inflammatory, and angiogenic activities, potentially shifting the surroundings from nonhealing to healing. The current review focuses on novel techniques, with a particular focus on recent revolutionary wound healing and infection control tactics based on nanomaterials, such as nanoparticles, nanocomposites, and scaffolds, which are discussed in depth. Furthermore, the effectiveness of nanoparticles as carriers for therapeutic compounds in wound-healing applications has been investigated which provide researchers an up-to-date sources on the use of nanomaterials and their creative ways that can improve wound-healing uses.
伤口处理不当影响到全世界数百万人,增加了死亡人数和费用。伤口有三个关键的并发症:(a)缺乏足够的细胞迁移、增殖和血管生成的环境;(b)微生物感染;(c)不稳定和长期炎症。遗憾的是,当代的治疗方法并没有完全解决这些基本困难,因此医学素养不足。多年来,纳米材料在伤口愈合方面的非凡能力已经取得了重大成功。纳米材料可以促进各种细胞和分子过程,通过抗菌、抗炎和血管生成活动来帮助伤口微环境,潜在地将环境从非愈合转变为愈合。目前的综述集中在新技术,特别关注最近基于纳米材料的革命性伤口愈合和感染控制策略,如纳米颗粒,纳米复合材料和支架,这些都进行了深入的讨论。此外,纳米颗粒作为治疗性化合物载体在伤口愈合应用中的有效性已被研究,这为研究人员提供了纳米材料使用的最新来源及其创造性方法,可以改善伤口愈合的用途。
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引用次数: 0
Acetylcholinesterase Activity in the Brain of Rats: Presence of an Inhibitor of Enzymatic Activity in Heliotropium eichwaldi L. Induced Silver/Gold Allied Bimetallic Nanoparticles 大鼠脑内乙酰胆碱酯酶活性:一种抑制银/金联合双金属纳米颗粒酶活性的抑制剂的存在
Q3 Engineering Pub Date : 2023-09-01 DOI: 10.26599/nbe.2023.9290034
Naila Sher, Mushtaq Ahmed, Nadia Mushtaq, Rahmat Ali khan
A simple green method for the production of silver/gold bimetallic nanoparticles (Ag/Au BNPs) using a Heliotropium eichwaldi L. (HE) extract was designed in this study. The reduction of Ag/Au metals to stable Ag/Au BNPs within 24 h at pH 5 using 1 mL of HE at 40 °C signifies a greater rate of reaction compared with chemically elaborated synthesis. The confirmation of the synthesis and the examination of the size, shape, and elemental composition of these BNPs were performed using visible absorption spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX). Stable, irregular-rod-shaped, and crystalline Ag/Au BNPs with a well-defined 6-nm diameter and a blue shift λmax of 532 nm were synthesized using the HE extract. Moreover, the anti-cholinesterase (anti-AChE) potential of the Ag/Au BNPs was tested as a treatment for Alzheimer’s disease (AD). An excellent anti-AChE activity (IC50, 71.2 ± 0.22 μg/mL) was observed for these biogenic-synthesized NPs. A statistical analysis revealed that Ag/Au BNPs inhibited AChE competitively, according to a Lineweaver–Burk plot, with Km increasing from 0.019 to 0.063 (288%–1185.7%) and Vmax remaining constant. The Ag/Au BNPs also caused an increase in KIapp, from 128 to 1184 (236%–828%), whereas they did not affect Vmaxiapp. The Km, KI, and Ki values were also calculated to be 0.0053 mmol/L, 595.25 µg, and 80 µg, respectively. Therefore, it is concluded that small-size, stable, and potent Ag/Au BNPs were synthesized successfully from HE extracts that exhibited anti-AChE activity, which renders them a significant remedy for AD.
本研究设计了一种简单的绿色生产银/金双金属纳米粒子(Ag/Au BNPs)的方法,该方法是利用Heliotropium eichwaldi L. (HE)提取物制备的。在pH值为5的条件下,在40°C下使用1ml HE,在24小时内将Ag/Au金属还原为稳定的Ag/Au BNPs,这表明与化学合成相比,反应速度更快。利用可见吸收光谱、傅里叶变换红外光谱(FT-IR)、x射线衍射(XRD)、扫描电子显微镜(SEM)和能量色散x射线光谱(EDX)对这些BNPs的合成进行了确认,并对其大小、形状和元素组成进行了检测。利用HE提取液合成了稳定的、不规则的棒状、结晶的Ag/Au BNPs,其直径为6 nm,蓝移λmax为532 nm。此外,测试了Ag/Au BNPs的抗胆碱酯酶(anti-AChE)潜能,作为阿尔茨海默病(AD)的治疗方法。这些生物合成的NPs具有良好的抗乙酰胆碱酯酶活性(IC50为71.2±0.22 μg/mL)。Lineweaver-Burk图显示,Ag/Au BNPs竞争性地抑制AChE, Km从0.019增加到0.063 (288% ~ 1185.7%),Vmax保持不变。Ag/Au BNPs也导致KIapp从128增加到1184(236%-828%),而它们对Vmaxiapp没有影响。Km、KI和KI值分别为0.0053 mmol/L、595.25µg和80µg。因此,从HE提取物中成功合成了小尺寸、稳定、有效的Ag/Au BNPs,具有抗ache活性,是治疗AD的重要药物。
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引用次数: 0
Detection of the Anticancer and Genotoxic Activities of Heliotropium eichwaldi L.-fabricated Silver Nanoparticles on BHK-21 Cells and Human Blood Lymphocytes Using MTT and Comet Assays MTT法和Comet法检测银纳米粒子对BHK-21细胞和人血淋巴细胞的抗癌和遗传毒性活性
Q3 Engineering Pub Date : 2023-09-01 DOI: 10.26599/nbe.2023.9290037
Naila Sher, Mushtaq Ahmed, Nadia Mushtaq, Rahmat Ali Khan
The possible treatment of cancer with nanoparticles (NPs) would be carried out via inoculation into the veins; as a result, the NPs would come into contact with white blood cells (WBCs) and red blood cells (RBCs) prior to reaching the target cancerous cells. In the current study, the genotoxicity and cytotoxicity potential of silver NPs (AgNPs) against human blood lymphocytes and baby hamster kidney-21 (BHK-21) cells was tested using comet and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assays, respectively. First, AgNPs were created using a Heliotropium eichwaldi L. (HE) extract. These AgNPs were then confirmed by ultraviolet–visible (UV–Vis) spectroscopy, which yielded a sharp peak at 416 nm with a maximum absorbance of 1.92. Moreover, an X-ray diffraction (XRD) analysis confirmed the crystalline nature and particle size (19.79 nm) of AgNPs, whereas scanning electron microscopy (SEM) revealed their irregular morphology and size of 30 nm. In turn, an EDX analysis indicated that AgNPs had an appreciable composition of Ag ions (30.68%). According to the comet assay, the HE-AgNPs, and standard H2O2 caused highly significant damage to DNA compared with HE extract. The comet assay was reported in terms of the total comet score (TCS). In the case of the MTT assay, a dose-dependent cytotoxicity was noted, in which doxorubicin, and AgNPs were more potent against BHK-21 cells compared with a plant extract. From these results, it is evident that the green-synthesized AgNPs interacted with blood lymphocytes and BHK-21 cells, caused damage to DNA via oxidative stress, and finally triggered cell death (apoptosis). However, further studies aimed at reducing their potential threats are recommended.
纳米颗粒(NPs)治疗癌症的可能方法是通过注射到静脉中进行;因此,NPs会在到达目标癌细胞之前先与白细胞(wbc)和红细胞(rbc)接触。本研究采用comet和3-[4,5-二甲基噻唑-2-基]-2,5二苯基溴化四氮唑(MTT)试验分别检测银NPs (AgNPs)对人血液淋巴细胞和幼鼠肾-21 (BHK-21)细胞的遗传毒性和细胞毒性。首先,利用Heliotropium eichwaldi L. (HE)提取物制备AgNPs。这些AgNPs通过紫外可见光谱(UV-Vis)得到了证实,在416 nm处有一个尖峰,最大吸光度为1.92。此外,x射线衍射(XRD)分析证实了AgNPs的结晶性质和粒径(19.79 nm),而扫描电子显微镜(SEM)显示其形状不规则,粒径为30 nm。反过来,EDX分析表明AgNPs具有可观的Ag离子组成(30.68%)。根据彗星试验,HE- agnps和标准H2O2与HE提取物相比,对DNA造成了非常显著的损伤。彗星试验是根据总彗星评分(TCS)进行报道的。在MTT试验的情况下,注意到剂量依赖性的细胞毒性,其中阿霉素和AgNPs对BHK-21细胞的作用比植物提取物更有效。从这些结果可以看出,绿色合成的AgNPs与血液淋巴细胞和BHK-21细胞相互作用,通过氧化应激导致DNA损伤,最终引发细胞死亡(凋亡)。然而,建议进一步研究以减少它们的潜在威胁。
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引用次数: 0
Application of Artificial Intelligence in the Exploration and Optimization of Biomedical Nanomaterials 人工智能在生物医用纳米材料探索与优化中的应用
Q3 Engineering Pub Date : 2023-09-01 DOI: 10.26599/nbe.2023.9290035
Xiaoyang Zhu, Yan Li, Ning Gu
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引用次数: 0
The Effects of Nano Magnetic Graphene Oxide on In Vivo Maturation of Oocyte 纳米磁性氧化石墨烯对卵母细胞体内成熟的影响
Q3 Engineering Pub Date : 2023-09-01 DOI: 10.26599/nbe.2023.9290036
Mitra Rahimi, Tahereh Foroutan, Fatemeh Eini
Graphene oxide (GO) and Fe3O4 super paramagnetic material are good candidate for some applications such as drug delivery. It has been shown that combining Fe3O4 with graphene oxide increases the biological efficiency of GO. The use of novel assisted reproductive technologies such as gonadotropins injection has been able to help the fertility of infertile people, but the side effects of these methods and high costs are still problems. The aim of the present study was to investigate the effect of magnetic graphene oxide (MGO) on the in vivo maturation of mouse oocytes. Thirty 6–8-week old female Naval Medical Research Institute (NMRI) mice were treated with intra peritoneal (I.P) injection of MGO mixed with hormones. 12 h after I.P. injection of MGO mixed with PMSG and HCG, the number of metaphase II (MII) oocytes obtained from the left fallopian tubes was counted in each group. Also, immuno-cytochemical staining of glutathione and morphometric analysis of ovaries were studied. The results of this study showed that the simultaneous use of MGO, pregnant mare serum gonadotropin (PMSG), and human chorionic gonadotrophin (HCG) increases the number of MII oocytes and helps to increase maturation of oocytes. It could be concluded that MGO can increase the efficiency of super ovulating hormones due to increase in adsorption of serum hormones and growth factors.
氧化石墨烯(GO)和Fe3O4超顺磁性材料是一些应用的良好候选者,如药物输送。研究表明,氧化石墨烯与Fe3O4结合可提高氧化石墨烯的生物效率。使用新型辅助生殖技术,如注射促性腺激素,已经能够帮助不孕患者的生育能力,但这些方法的副作用和高昂的费用仍然是问题。本研究的目的是探讨磁性氧化石墨烯(MGO)对小鼠卵母细胞体内成熟的影响。本研究采用MGO混合激素腹腔注射治疗30只6 ~ 8周龄海军医学研究所(NMRI)雌性小鼠。注射MGO混合PMSG和HCG 12 h后,计数各组左输卵管中期II (MII)卵母细胞数量。同时对卵巢进行谷胱甘肽免疫细胞化学染色和形态计量学分析。本研究结果表明,同时使用MGO、孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG)可增加MII卵母细胞数量,并有助于促进卵母细胞成熟。由此可见,MGO可通过增加对血清激素和生长因子的吸附来提高超排卵激素的效率。
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引用次数: 0
Synthesis of Ag 2O Nanoparticles via Fresh Pomegranate Peel Extract for Bioapplications 新鲜石榴皮提取物合成Ag2O纳米粒子及其生物应用
Q3 Engineering Pub Date : 2023-08-01 DOI: 10.26599/nbe.2023.9290032
Wedian K. Abad, A. N. Abd, N. Habubi
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引用次数: 0
Biosynthesis of Zinc Oxide Nanoparticles Using Punica granatum L. Waste Peel Extract, and Assessment of Antioxidant and Catalytic Activity 石榴皮废料提取物生物合成氧化锌纳米粒子及其抗氧化和催化活性评价
Q3 Engineering Pub Date : 2023-08-01 DOI: 10.26599/nbe.2023.9290030
M. H. Meshkatalsadat, Alireza Momeni, Mohammad Reza Abdollahzadeh
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引用次数: 0
期刊
Nano Biomedicine and Engineering
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