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Epidemiology of Tinea Capitis Among School-Children in Dschang, Western Cameroon. 喀麦隆西部 Dschang 学童头癣流行病学。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-12 DOI: 10.1007/s11046-024-00857-y
D A J Agokeng, S Dabou, J Kabtani, K B D Agokeng, K Diongue, G S S Njateng, S Ranque

Data on the epidemiology of tinea capitis (TC), an infection of the scalp by dermatophytes, are scarce in Cameroon. This study aimed to determine the prevalence of TC among school-children in the Dschang Subdivision, Western Cameroon. A cross-sectional study was carried out in June 2021 in Dschang including pupils aged 5-13. First, a standardized questionnaire was administered to participant for the collection of sociodemographic data. Then, samples were collected and cultured onto Sabouraud-Chloramphenicol-Gentamicin Agar. The etiological agents were identified based on their morphological features and with MALDI-TOF mass spectrometry. A total of 1070 children were clinically examined and 108 (10.1%) children presented with TC lesions. The mean age of the 1070 participants was 8.3 ± 2.6 years (range: 5-13 years); 772 (72.2%) were males. The use of borehole water (OR = 0.01, 95%CI[0.001-0.03]), spring water (OR = 0.2, 95%CI[0.08-0.50]), rainwater (OR = 0.004, 95%CI[0.001-0.016]), and hairdressing salons visits (OR = 0.413, 95%CI[0.196-0.872]) were associated with a decreased TC risk in the multivariate logistic regression analysis. In contrast, sharing bed with siblings (OR = 4.48, 95%CI[2.095-9.60]) was associated with an increased TC risk in children. Among the 32 dermatophytes isolated in culture, Microsporum audouinii was the most frequent (43.8%), followed by Trichophyton rubrum (25.0%) and T. soudanense (25.0%). Microsporum canis and T. violaceum were both rarely isolated. Further studies are warranted to assess the association of TC with domestic water usage that has been highlighted in this study.

头癣(TC)是一种由皮癣菌引起的头皮感染,在喀麦隆有关头癣流行病学的数据很少。本研究旨在确定喀麦隆西部Dschang分区学龄儿童中头癣的发病率。这项横断面研究于 2021 年 6 月在 Dschang 开展,包括 5-13 岁的学生。首先,对参与者进行了标准化问卷调查,以收集社会人口学数据。然后,收集样本并在沙保律-氯霉素-庆大霉素琼脂上进行培养。根据病原体的形态特征并利用 MALDI-TOF 质谱法对其进行鉴定。共对 1070 名儿童进行了临床检查,其中 108 名儿童(10.1%)出现 TC 病变。1070 名参与者的平均年龄为 8.3 ± 2.6 岁(5-13 岁),其中 772 名(72.2%)为男性。在多变量逻辑回归分析中,使用井水(OR = 0.01,95%CI[0.001-0.03])、泉水(OR = 0.2,95%CI[0.08-0.50])、雨水(OR = 0.004,95%CI[0.001-0.016])和去理发店(OR = 0.413,95%CI[0.196-0.872])与TC风险降低有关。与此相反,与兄弟姐妹同床(OR = 4.48,95%CI[2.095-9.60])与儿童 TC 风险增加有关。在培养分离出的 32 种皮癣菌中,奥杜茵小孢子菌最常见(43.8%),其次是红毛癣菌(25.0%)和苏旦癣菌(25.0%)。犬小孢子菌和小毛癣菌很少被分离出来。有必要进行进一步研究,以评估本研究中强调的癣菌与家庭用水的关系。
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引用次数: 0
Multifocal Sporotrichosis Associated with Armadillo Hunting in Midwest Brazil: An In-Depth Case Study and Comprehensive Literature Analysis. 巴西中西部地区与犰狳狩猎有关的多灶性孢子丝虫病:一项深入的案例研究和全面的文献分析。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-12 DOI: 10.1007/s11046-024-00854-1
Anderson Messias Rodrigues, Jamile Ambrósio de Carvalho, Andreia Ferreira Nery, Marcia Hueb, Ivana Garcia, Armando Guevara, Zoilo Pires de Camargo, Rosane Christine Hahn

Sporotrichosis is a globally distributed subcutaneous mycosis caused by dimorphic Sporothrix species commonly found in soil, mosses, and decaying plant matter. The lymphocutaneous manifestation, historically associated with occupational activities and sapronotic transmission, has recently been observed to also occur through animal contact, particularly notable in Brazil. We describe a rare case of lymphocutaneous sporotrichosis with simultaneous pulmonary complications resulting from the scratching of a southern three-banded armadillo, Tolypeutes matacus, primarily inhabiting the arid forests of South America's central region. Speciation using multiplex quantitative polymerase chain reaction (qPCR) established the etiological agent as S. schenckii s. str., while amplified fragment length polymorphism (AFLP) analysis unveiled a novel genotype circulating in the Midwest of Brazil. The patient received treatment with itraconazole (200 mg/day) for two months, leading to substantial clinical improvement of cutaneous and pulmonary symptoms. This case highlights the critical role of animal-mediated transmission in sporotrichosis epidemiology, particularly within regions with diverse armadillo species. The unusual epidemiology and genetic characteristics of this case emphasize the need for enhanced awareness and diagnostic vigilance in atypical sporotrichosis presentations.

孢子丝菌病是一种分布于全球的皮下真菌病,由常见于土壤、苔藓和腐烂植物中的二形孢子丝菌引起。淋巴皮肤表现历来与职业活动和无菌传播有关,但最近观察到也可通过动物接触发生,这在巴西尤为显著。我们描述了一例罕见的淋巴皮肤孢子丝菌病病例,该病例因搔抓主要栖息于南美洲中部干旱森林的南方三带犰狳(Tolypeutes matacus)而引起,同时伴有肺部并发症。利用多重定量聚合酶链反应(qPCR)进行的物种鉴定确定了病原体为S. schenckii s. str.,而扩增片段长度多态性(AFLP)分析则揭示了一种在巴西中西部地区流行的新型基因型。患者接受了为期两个月的伊曲康唑(200 毫克/天)治疗,皮肤和肺部症状得到了显著改善。该病例凸显了动物媒介传播在孢子丝菌病流行病学中的关键作用,尤其是在犰狳种类繁多的地区。该病例不寻常的流行病学和遗传特征强调了在非典型孢子丝菌病病例中提高意识和诊断警惕的必要性。
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引用次数: 0
Rethinking Aspergillosis in the Era of Microbiota and Mycobiota. 在微生物群和霉菌生物群时代重新思考曲霉菌病。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-12 DOI: 10.1007/s11046-024-00853-2
Aleksandra Barac, Ankica Vujovic, Jovan Peric, Ivan Tulic, Maja Stojanovic, Mihailo Stjepanovic

Aspergillosis encompasses a wide range of clinical conditions based on the interaction between Aspergillus and the host. It ranges from colonization to invasive aspergillosis. The human lung provides an entry door for Aspergillus. Aspergillus has virulence characteristics such as conidia, rapid growth at body temperature, and the production of specific proteins, carbohydrates, and secondary metabolites that allow A. fumigatus to infiltrate the lung's alveoli and cause invasive aspergillosis. Alveolar epithelial cells play an important role in both fungus clearance and immune cell recruitment via cytokine release. Although the innate immune system quickly clears conidia in immunocompetent hosts, A. fumigatus has evolved multiple virulence factors in order to escape immune response such as ROS detoxifying enzymes, the rodlet layer, DHN-melanin and toxins. Bacterial co-infections or interactions can alter the immune response, impact Aspergillus growth and virulence, enhance biofilm formation, confound diagnosis, and reduce treatment efficacy. The gut microbiome's makeup influences pulmonary immune responses generated by A. fumigatus infection and vice versa. The real-time PCR for Aspergillus DNA detection might be a particularly useful tool to diagnose pulmonary aspergillosis. Metagenomics analyses allow quick and easy detection and identification of a great variety of fungi in different clinical samples, although optimization is still required particularly for the use of NGS techniques. This review will analyze the current state of aspergillosis in light of recent discoveries in the microbiota and mycobiota.

曲霉菌病包括各种基于曲霉菌与宿主相互作用的临床症状。它包括从定植到侵袭性曲霉菌病。人的肺部是曲霉菌的入口。曲霉菌具有分生孢子、在体温下快速生长以及产生特定蛋白质、碳水化合物和次生代谢物等毒力特征,这些特征使得烟曲霉菌能够渗入肺泡并引起侵袭性曲霉菌病。肺泡上皮细胞在清除真菌和通过释放细胞因子招募免疫细胞方面都发挥着重要作用。虽然先天性免疫系统能迅速清除免疫功能正常宿主的分生孢子,但烟曲霉已进化出多种毒力因子,以逃避免疫反应,如 ROS 解毒酶、小杆层、DHN-黑色素和毒素。细菌合并感染或相互作用会改变免疫反应,影响曲霉菌的生长和毒力,促进生物膜的形成,混淆诊断并降低治疗效果。肠道微生物组的构成会影响烟曲霉感染产生的肺部免疫反应,反之亦然。用于检测曲霉菌 DNA 的实时 PCR 可能是诊断肺曲霉菌病的一种特别有用的工具。元基因组学分析可快速、简便地检测和鉴定不同临床样本中的多种真菌,但仍需优化,特别是在使用 NGS 技术时。本综述将根据微生物群和真菌生物群的最新发现分析曲霉菌病的现状。
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引用次数: 0
Utility of Itraconazole in Combination with Liposomal Amphotericin B in Rhizopus oryzae Associated Mucormycosis-An Exploratory Study. 伊曲康唑与两性霉素 B 脂质体联合治疗根霉菌性粘液瘤病的效用--一项探索性研究
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-12 DOI: 10.1007/s11046-024-00859-w
Himanshu Dandu, Naveen Kumar, Neeraj Kumar, Hardeep Singh Malhotra, Vikas Prabhu, Vipin Raj Bharti, Prashant Gupta, Geeta Yadav, Veerendra Verma

Background: During the COVID-19 pandemic-associated mucor epidemic, acute antifungal drug shortage necessitated the exploration of other antifungals based on culture sensitivity. Itraconazole is a cheap, safe, and effective antifungal in sensitive cases.

Methodology: We enrolled itraconazole-sensitive COVID-19-associated mucormycosis during the mucormycosis pandemic. After the intensive phase course of liposomal amphotericin B, Itraconazole was offered in susceptible cases during the maintenance phase along with standard of care. These patients were clinically and radiologically followed for 6 months.

Results: We enrolled 14 patients (Male: Female-11:3) of Rhino-orbito-cerebral mucormycosis (ROCM) which included 12 diabetics. All patients had facial swelling, orbital swelling, visual impairment, and headache. MRI showed involvement of bilateral sinus (10/14), orbital extension (13/14), cavernous sinus (5/14), cerebral part of the internal carotid artery (3/14), and brain infarcts (4/14). All 14 patients showed sensitivity to Itraconazole with 12 having minimum inhibitory concentration (MIC) ≤ 1 μg/ml and 2 having MIC ≤ 2 μg/ml. Follow-up at 6 months showed clinical improvement in the majority (11/14) and radiological improvement in six out of seven scanned patients.

Conclusion: Our study shows the potential therapeutic role of oral Itraconazole in ROCM.

背景:在COVID-19大流行病相关的粘孢子菌流行期间,由于抗真菌药物严重短缺,必须根据培养敏感性探索其他抗真菌药物。伊曲康唑是一种廉价、安全且对敏感病例有效的抗真菌药物:在粘孢子菌病大流行期间,我们收治了对伊曲康唑敏感的 COVID-19 相关粘孢子菌病患者。在脂质体两性霉素 B 强化阶段疗程后,伊曲康唑与标准护理一起用于易感病例的维持阶段。我们对这些患者进行了为期 6 个月的临床和影像学随访:我们共收治了 14 名鼻-眼-脑粘液瘤病 (ROCM) 患者(男:女:11:3),其中包括 12 名糖尿病患者。所有患者均有面部肿胀、眼眶肿胀、视力障碍和头痛。磁共振成像显示双侧鼻窦(10/14)、眼眶扩展(13/14)、海绵窦(5/14)、颈内动脉大脑部分(3/14)和脑梗塞(4/14)受累。所有 14 名患者均对伊曲康唑敏感,其中 12 人的最低抑菌浓度 (MIC) ≤ 1 μg/ml,2 人的最低抑菌浓度 (MIC) ≤ 2 μg/ml。6 个月的随访显示,大多数患者(11/14)的临床症状有所改善,7 名扫描患者中有 6 名的放射学症状有所改善:我们的研究显示了口服伊曲康唑对 ROCM 的潜在治疗作用。
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引用次数: 0
Azole Resistance in Veterinary Clinical Aspergillus fumigatus Isolates in the Netherlands. 荷兰兽医临床曲霉菌分离物中的唑类抗药性。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-12 DOI: 10.1007/s11046-024-00850-5
Marloes A M van Dijk, Jochem B Buil, Marlou Tehupeiory-Kooreman, Marian J Broekhuizen, Els M Broens, Jaap A Wagenaar, Paul E Verweij

Aspergillus fumigatus is a saprophytic fungal pathogen that causes opportunistic infections in animals and humans. Azole resistance has been reported globally in human A. fumigatus isolates, but the prevalence of resistance in isolates from animals is largely unknown. A retrospective resistance surveillance study was performed using a collection of clinical A. fumigatus isolates from various animal species collected between 2015 and 2020. Agar-based azole resistance screening of all isolates was followed by in vitro antifungal susceptibility testing and cyp51A gene sequencing of the azole-resistant isolates. Over the 5 year period 16 (11.3%) of 142 A. fumigatus culture-positive animals harbored an azole-resistant isolate. Resistant isolates were found in birds (15%; 2/13), cats (21%; 6/28), dogs (8%; 6/75) and free-ranging harbor porpoise (33%; 2/6). Azole-resistance was cyp51A mediated in all isolates: 81.3% (T-67G/)TR34/L98H, 12.5% TR46/Y121F/T289A. In one azole-resistant A. fumigatus isolate a combination of C(-70)T/F46Y/C(intron7)T/C(intron66)T/M172V/E427K single-nucleotide polymorphisms in the cyp51A gene was found. Of the animals with an azole-resistant isolate and known azole exposure status 71.4% (10/14) were azole naive. Azole resistance in A. fumigatus isolates from animals in the Netherlands is present and predominantly cyp51A TR-mediated, supporting an environmental route of resistance selection. Our data supports the need to include veterinary isolates in resistance surveillance programs. Veterinarians should consider azole resistance as a reason for therapy failure when treating aspergillosis and consider resistance testing of relevant isolates.

烟曲霉(Aspergillus fumigatus)是一种吸附性真菌病原体,可引起动物和人类的机会性感染。据报道,全球范围内的人类烟曲霉分离物都对唑类药物产生了抗药性,但动物分离物的抗药性流行情况却不为人知。我们利用 2015 年至 2020 年间从不同动物物种中收集到的临床烟曲霉分离株进行了一项回顾性耐药性监测研究。在对所有分离株进行琼脂基唑类耐药性筛选后,又对耐唑类分离株进行了体外抗真菌药敏试验和 cyp51A 基因测序。在 5 年的时间里,142 个烟曲霉培养阳性动物中有 16 个(11.3%)携带耐唑分离物。在鸟类(15%;2/13)、猫类(21%;6/28)、狗类(8%;6/75)和自由放养的港湾鼠海豚(33%;2/6)中都发现了耐药性分离株。所有分离物对唑类的抗药性都是由 cyp51A 介导的:81.3% (t-67g/)tr34/l98h, 12.5% tr46/y121f/t289a。在一个抗唑烟曲霉分离株中,发现了 cyp51A 基因中的 C(-70)T/F46Y/C(intron7)T/C(intron66)T/M172V/E427K 单核苷酸多态性组合。在分离出抗偶氮唑且已知偶氮唑暴露状态的动物中,71.4%(10/14)对偶氮唑不敏感。荷兰动物中分离出的烟曲霉菌具有唑耐药性,且主要由 cyp51A TR 介导,这支持了耐药性选择的环境途径。我们的数据支持将兽医分离物纳入耐药性监测计划的必要性。兽医在治疗曲霉菌病时应将唑类耐药性视为治疗失败的原因之一,并考虑对相关分离物进行耐药性检测。
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引用次数: 0
High Prevalence of Terbinafine Resistance Among Trichophyton mentagrophytes/T. interdigitale Species Complex, a Cross-Sectional Study from 2021 to 2022 in Northern Parts of Iran. 2021年至2022年在伊朗北部地区进行的一项横断面研究发现,对特比萘芬(Terbinafine)产生抗药性的毛癣菌/T. interdigitale复合菌种的流行率很高。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-12 DOI: 10.1007/s11046-024-00855-0
Iman Haghani, Maryam Babaie, Akbar Hoseinnejad, Ali Rezaei-Matehkolaei, Ramin Mofarrah, Zahra Yahyazadeh, Firoozeh Kermani, Javad Javidnia, Tahereh Shokohi, Maryam Azish, Kambiz Kamyab Hesari, Majid Saeedi, Zeinab Ghasemi, Shaghayegh Khojasteh, Zohreh Hajheydari, Elham Mosayebi, Reza Valadan, Seyedmojtaba Seyedmousavi, Mahdi Abastabar, Mohammad Taghi Hedayati

Treatment-resistant dermatophytosis caused by the members of the Trichophyton mentagrophytes/Trichophyton interdigitale species group (TMTISG) is increasing worldwide. We aimed to determine the prevalence of TMTISG in patients with dermatophytosis in two centers from north of Iran and detect the possible mutations in the squalene epoxidase (SQLE) gene in relevant terbinafine (TRB) resistant pathogenic isolates. From November 2021 to December 2022, 1960 patients suspected to dermatophytosis and referred to two mycology referral laboratories in the north of Iran were included in the study. Identification of all dermatophyte isolates was confirmed by RFLP of rDNA internal transcribed spacer (ITS) regions. Antifungal susceptibility testing against five common antifungals using the CLSI-M38-A3 protocol was performed. The TMTISG isolates resistant to TRB, were further analyzed to determine the possible mutations in the SQLE gene. Totally, 647 cases (33%) were positive for dermatophytosis of which 280 cases (43.3%) were identified as members of TMTISG. These were more frequently isolated from tinea corporis 131 (44.56%) and tinea cruris 116 (39.46%). Of 280 TMTISG isolates, 40 (14.3%) were resistant to TRB (MIC ≥ 4 µg/mL), all found to be T. indotineae in ITS sequencing. In SQLE sequencing 34 (85%) of TRB-resistant isolates had coincident mutations of Phe397Leu and Ala448Thr whereas four and two isolates had single mutations of Phe397Leu and Leu393Ser, respectively. Overall, the resistance of Iranian TMTISG isolates to TRB greatly occurred by a mutation of Phe397Leu in the SQLE gene as alone or in combination with Ala448Thr. Nevertheless, for the occurrence of in vitro resistance, only the presence of Phe397Leu mutation seems to be decisive.

由脑毛癣菌/间黑毛癣菌物种群(TMTISG)成员引起的耐药性皮癣病在全球范围内呈上升趋势。我们的目的是确定伊朗北部两个中心的皮癣患者中 TMTISG 的流行率,并检测相关特比萘芬(TRB)耐药病原分离物中角鲨烯环氧化物酶(SQLE)基因可能发生的突变。从 2021 年 11 月到 2022 年 12 月,伊朗北部两个真菌学转诊实验室共接收了 1960 名疑似皮癣病患者。所有皮癣菌分离物的鉴定均通过 rDNA 内部转录间隔区 (ITS) 的 RFLP 进行确认。根据 CLSI-M38-A3 协议,对五种常见抗真菌药物进行了抗真菌药敏试验。对TRB耐药的TMTISG分离物进行了进一步分析,以确定SQLE基因中可能存在的突变。总共有 647 个病例(33%)对皮肤癣菌病呈阳性反应,其中 280 个病例(43.3%)被确定为 TMTISG 成员。其中,131 例(44.56%)和 116 例(39.46%)从体癣中分离出了 TMTISG。在 280 个 TMTISG 分离物中,有 40 个(14.3%)对 TRB 具有耐药性(MIC ≥ 4 µg/mL),在 ITS 测序中发现它们都是靛基癣菌(T. indotineae)。在 SQLE 测序中,34 个(85%)对 TRB 耐药的分离株同时发生了 Phe397Leu 和 Ala448Thr 突变,而 4 个和 2 个分离株分别发生了 Phe397Leu 和 Leu393Ser 突变。总体而言,伊朗 TMTISG 分离物对 TRB 的抗药性主要是由 SQLE 基因中的 Phe397Leu 突变单独或与 Ala448Thr 结合产生的。然而,对于体外抗药性的产生,似乎只有 Phe397Leu 突变的存在才是决定性的。
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引用次数: 0
Management of Mucormycosis Caused by Rhizopus arrhizus in a Child with Severe Aplastic Anemia and Diabetes Mellitus Undergoing Transplantation. 对一名接受移植手术的重型再生障碍性贫血和糖尿病患儿由根瘤蚜引起的粘孢子菌病的治疗。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-12 DOI: 10.1007/s11046-024-00860-3
Zühre Kaya, Serap Kirkiz, Büşra Topuz Türkcan, Sidre Erganiş, Ayşe Kalkancı, Pınar Uyar, Akif Muhtar Öztürk, Hasan Tezer, Ülker Koçak
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引用次数: 0
Aspergillosis in Critically Ill Patients with and Without COVID-19 in a Tertiary Hospital in Southern Brazil. 巴西南部一家三甲医院患有和未患有 COVID-19 的重症患者中的曲霉菌病。
IF 5.5 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-07 DOI: 10.1007/s11046-024-00862-1
Mariana Rodrigues Trápaga, Vanice Rodrigues Poester, Rossana Patrícia Basso, Bianca Dos Santos Blan, Lívia Silveira Munhoz, Alessandro C Pasqualotto, Talita da Fontoura Werner, Maria Letícia Figurelli, David A Stevens, Andrea von Groll, Melissa Orzechowski Xavier

The impact of invasive pulmonary aspergillosis (IPA) on non-neutropenic critically ill patients in intensive care units (ICU) has been demonstrated in recent decades. Furthermore, after the start of the COVID-19 pandemic, COVID-19 associated with pulmonary aspergillosis (CAPA) has become a major concern in ICUs. However, epidemiological data from different regions are scarce. We evaluated the prevalence and clinical-epidemiological data of IPA in patients with COVID-19 requiring mechanical ventilation (MV) in the ICU ("severe COVID-19") and non-COVID ICU patients in MV of a tertiary hospital in the southern region of Brazil. Eighty-seven patients admitted between June 2020 and August 2022 were included; 31 with severe COVID-19. For the diagnosis of IPA or CAPA, algorithms including host factors and mycological criteria (positive culture for Aspergillus spp., immunoassay for galactomannan detection, and/or qPCR) were utilized. The overall incidence of IPA and CAPA in our ICU was 73 cases/1000 ICU hospitalizations. Aspergillosis occurred in 13% (4/31) of the COVID-19 patients, and in 16% (9/56) of the critically ill patients without COVID-19, with mortality rates of 75% (3/4) and 67% (6/9), respectively. Our results highlight the need for physicians enrolled in ICU care to be aware of aspergillosis and for more access of the patients to sensitive and robust diagnostic tests by biomarkers detection.

近几十年来,侵袭性肺曲霉菌病(IPA)对重症监护病房(ICU)中非中性粒细胞减少的重症患者的影响已得到证实。此外,在 COVID-19 大流行开始后,COVID-19 相关肺曲霉菌病(CAPA)已成为重症监护病房的主要关注点。然而,来自不同地区的流行病学数据却很少。我们评估了巴西南部地区一家三甲医院需要在重症监护室进行机械通气(MV)的 COVID-19 患者("重症 COVID-19")和非 COVID ICU MV 患者中 IPA 的发病率和临床流行病学数据。研究纳入了2020年6月至2022年8月期间入院的87名患者,其中31人患有重度COVID-19。在诊断IPA或CAPA时,采用了包括宿主因素和真菌学标准(曲霉菌属培养阳性、半乳糖甘露聚糖免疫测定和/或qPCR)在内的算法。在我们的重症监护病房中,IPA和CAPA的总发病率为73例/1000例重症监护病房住院患者。COVID-19患者中有13%(4/31)发生了曲霉菌病,没有COVID-19的重症患者中有16%(9/56)发生了曲霉菌病,死亡率分别为75%(3/4)和67%(6/9)。我们的研究结果突出表明,参与重症监护室护理的医生需要了解曲霉菌病,并通过生物标记物检测为患者提供更多灵敏、可靠的诊断测试。
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引用次数: 0
Trichophyton mentagrophytes Tinea of Vellus Hair Misdiagnosed as Eczema. 毛囊发癣菌被误诊为湿疹。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-05 DOI: 10.1007/s11046-024-00863-0
Li Zhang, Xiujiao Xia, Xiaohang Du, Xiuzu Song
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引用次数: 0
Aspergillus hubkae, a Novel Species Isolated from a Patient with Probable Invasive Pulmonary Aspergillosis. 从一名可能患有侵袭性肺部曲霉菌病的患者身上分离出的新菌种--Aspergillus hubkae。
IF 5.5 3区 生物学 Q1 Veterinary Pub Date : 2024-05-11 DOI: 10.1007/s11046-024-00848-z
Ya Bin Zhou, Ali Rezaei-Matehkolaei, Martin Meijer, Bart Kraak, Bert Gerrits van den Ende, Ferry Hagen, Sara Afzalzadeh, Neda Kiasat, Ameneh Takesh, Akbar Hoseinnejad, Jos Houbraken

A 50-year-old man, previously diagnosed with pulmonary tuberculosis and lung cavities, presented with symptoms including fever, shortness of breath, and cough. A pulmonary CT scan revealed multiple cavities, consolidation and tree-in-bud in the upper lungs. Further investigation through direct examination of bronchoalveolar lavage fluid showed septate hyphae with dichotomous acute branching. Subsequent isolation and morphological analysis identified the fungus as belonging to Aspergillus section Nigri. The patient was diagnosed with probable invasive pulmonary aspergillosis and successfully treated with a three-month oral voriconazole therapy. Phylogenetic analysis based on partial β-tubulin, calmodulin and RNA polymerase second largest subunit sequences revealed that the isolate represents a putative new species related to Aspergillus brasiliensis, and is named Aspergillus hubkae here. Antifungal susceptibility testing demonstrated that the isolate is resistant to itraconazole but susceptible to voriconazole. This phenotypic and genetic characterization of A. hubkae, along with the associated case report, will serve as a valuable resource for future diagnoses of infections caused by this species. It will also contribute to more precise and effective patient management strategies in similar clinical scenarios.

一名曾被诊断患有肺结核和肺空洞的 50 岁男子出现了发烧、气短和咳嗽等症状。肺部 CT 扫描显示上肺有多个空洞、合并症和树状脓疱。通过对支气管肺泡灌洗液的直接检查进行进一步调查,发现了具有二歧急性分支的隔膜菌丝。经过分离和形态学分析,确定该真菌属于黑曲霉。患者被诊断为可能的侵袭性肺曲霉菌病,并成功接受了为期三个月的伏立康唑口服治疗。根据部分β-微管蛋白、钙调蛋白和RNA聚合酶第二大亚基序列进行的系统发育分析表明,该分离株代表了与巴西曲霉有关的推定新种,在此命名为Hubkae曲霉。抗真菌药敏试验表明,该分离株对伊曲康唑有抗药性,但对伏立康唑敏感。对 A. hubkae 进行的表型和基因鉴定以及相关的病例报告,将为今后诊断由该菌种引起的感染提供宝贵的资料。它还将有助于在类似的临床情况下制定更精确、更有效的患者管理策略。
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Mycopathologia
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