首页 > 最新文献

Mycopathologia最新文献

英文 中文
Deep Trichophytosis Mimiking Superinfected Bacterial Folliculitis. 模仿细菌性毛囊炎超级感染的深部毛囊炎
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-07-05 DOI: 10.1007/s11046-024-00872-z
Lina Weiss, Sara E Cerminara, Riccardo Curatolo, Hazem A Juratli, Matiar Madanchi
{"title":"Deep Trichophytosis Mimiking Superinfected Bacterial Folliculitis.","authors":"Lina Weiss, Sara E Cerminara, Riccardo Curatolo, Hazem A Juratli, Matiar Madanchi","doi":"10.1007/s11046-024-00872-z","DOIUrl":"10.1007/s11046-024-00872-z","url":null,"abstract":"","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"189 4","pages":"62"},"PeriodicalIF":3.6,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11226507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141534865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Susceptibility Testing of Environmental and Clinical Aspergillus sydowii Demonstrates Potent Activity of Various Antifungals. 环境和临床西多威曲霉的药敏试验显示了多种抗真菌药物的强效作用。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-07-03 DOI: 10.1007/s11046-024-00869-8
Bram Spruijtenburg, Antonio Rezusta, Jos Houbraken, Ferry Hagen, Theun de Groot, Jacques F Meis, Eelco F J Meijer

The genus Aspergillus consists of a vast number of medically and environmentally relevant species. Aspergillus species classified in series Versicolores are ubiquitous in the environment and include the opportunistic pathogen Aspergillus sydowii, which is associated with onychomycosis and superficial skin infections. Despite frequent clinical reports of A. sydowii and related series Versicolores species, antifungal susceptibility data are scarce, hampering optimal treatment choices and subsequent patient outcomes. Here, we employed antifungal susceptibility testing (AFST) based on microbroth dilution on a set of 155 series Versicolores strains using the common antifungals amphotericin B, itraconazole, voriconazole, posaconazole, isavuconazole and micafungin with the addition of luliconazole and olorofim. All strains were identified using partial calmodulin gene sequencing, with 145 being A. sydowii, seven A. creber and three A. versicolor, using the latest taxonomic insights. Overall, tested antifungals were potent against the entire strain collection. In comparison to A. fumigatus, azole and amphotericin B MICs were slightly elevated for some strains. AFST with luliconazole and olorofim, here reported for the first time, displayed the highest in vitro activity, making these antifungals interesting alternative drugs but clinical studies are warranted for future therapeutic use.

曲霉属由大量与医学和环境相关的菌种组成。Versicolores系列曲霉菌在环境中无处不在,其中包括机会性病原体Aspergillus sydowii,它与甲癣和表皮感染有关。尽管临床上经常有关于A. sydowii和相关系列Versicolores菌的报道,但抗真菌药敏数据却很少,这影响了最佳治疗选择和患者的后续治疗效果。在此,我们采用微流稀释法对 155 株 Versicolores 系列菌株进行了抗真菌药敏试验(AFST),使用了两性霉素 B、伊曲康唑、伏立康唑、泊沙康唑、异武康唑和米卡芬等常用抗真菌药,并添加了卢立康唑和奥罗芬。所有菌株都通过部分钙调蛋白基因测序进行了鉴定,其中 145 株为 A. sydowii,7 株为 A. creber,3 株为 A. versicolor。总体而言,测试的抗真菌药物对所有菌株都有很强的抑制作用。与烟曲霉相比,某些菌株的唑类和两性霉素 B MIC 值略有升高。首次报道的氟环唑和奥罗芬的 AFST 体外活性最高,使这些抗真菌药物成为有趣的替代药物,但未来的治疗用途还需要进行临床研究。
{"title":"Susceptibility Testing of Environmental and Clinical Aspergillus sydowii Demonstrates Potent Activity of Various Antifungals.","authors":"Bram Spruijtenburg, Antonio Rezusta, Jos Houbraken, Ferry Hagen, Theun de Groot, Jacques F Meis, Eelco F J Meijer","doi":"10.1007/s11046-024-00869-8","DOIUrl":"10.1007/s11046-024-00869-8","url":null,"abstract":"<p><p>The genus Aspergillus consists of a vast number of medically and environmentally relevant species. Aspergillus species classified in series Versicolores are ubiquitous in the environment and include the opportunistic pathogen Aspergillus sydowii, which is associated with onychomycosis and superficial skin infections. Despite frequent clinical reports of A. sydowii and related series Versicolores species, antifungal susceptibility data are scarce, hampering optimal treatment choices and subsequent patient outcomes. Here, we employed antifungal susceptibility testing (AFST) based on microbroth dilution on a set of 155 series Versicolores strains using the common antifungals amphotericin B, itraconazole, voriconazole, posaconazole, isavuconazole and micafungin with the addition of luliconazole and olorofim. All strains were identified using partial calmodulin gene sequencing, with 145 being A. sydowii, seven A. creber and three A. versicolor, using the latest taxonomic insights. Overall, tested antifungals were potent against the entire strain collection. In comparison to A. fumigatus, azole and amphotericin B MICs were slightly elevated for some strains. AFST with luliconazole and olorofim, here reported for the first time, displayed the highest in vitro activity, making these antifungals interesting alternative drugs but clinical studies are warranted for future therapeutic use.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"189 4","pages":"61"},"PeriodicalIF":3.6,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11222195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Systematic Review of Candidemia in Brazil: Unlocking Historical Trends and Challenges in Conducting Surveys in Middle-Income Countries. 巴西念珠菌病系统回顾:揭示历史趋势和在中等收入国家开展调查所面临的挑战》。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-28 DOI: 10.1007/s11046-024-00867-w
Flávio Guinsburg Hamburger, Ana Cristina Gales, Arnaldo Lopes Colombo

Introduction: Candidemia, a bloodstream infection predominantly affecting critically ill patients, poses a significant global health threat especially with the emergence of non-albicans Candida species, including drug-resistant strains. In Brazil, limited access to advanced diagnostic tools and trained microbiologists hampers accurate identification of Candida species and susceptibility to antifungals testing hindering surveillance efforts.

Methods: We conducted a systematic review spanning publications from 2017 to 2023 addressing Candida species distribution and antifungal susceptibility among Brazilian patients with candidemia.

Results: Despite initially identifying 7075 records, only 16 met inclusion criteria providing accurate information of 2305 episodes of candidemia. The predominant species were C. albicans, C. parapsilosis, and C. tropicalis, followed by notable proportions of Nakaseomyces glabratus. Limited access to diagnostic tests was evident as only 5 out of 16 studies on candidemia were able to report antifungal susceptibility testing results. In vitro resistance to echinocandins was rare (only 6/396 isolates, 1,5%). In counterpart, fluconazole exhibited resistance rates ranging from 0 to 43%, with great heterogeneity among different studies and species of Candida considered.

Conclusion: Our review underscores the critical need for enhanced surveillance and research efforts to address the evolving landscape of candidemia and antifungal resistance in Brazil. Despite some limitations, available data suggest that while resistance to echinocandins and amphotericin B remains rare, there is a growing concern regarding resistance to fluconazole among Candida species.

导言:念珠菌血症是一种主要影响危重病人的血流感染,对全球健康构成重大威胁,尤其是随着包括耐药菌株在内的非厌氧菌念珠菌的出现。在巴西,先进的诊断工具和训练有素的微生物学家有限,妨碍了念珠菌种类的准确鉴定和抗真菌药物敏感性测试,阻碍了监测工作:我们对2017年至2023年期间有关巴西念珠菌血症患者中念珠菌种类分布和抗真菌药物敏感性的出版物进行了系统回顾:尽管最初确定了 7075 条记录,但只有 16 条符合纳入标准,提供了 2305 例念珠菌血症的准确信息。主要菌种是白念珠菌、副丝状念珠菌和热带念珠菌,其次是明显比例的光滑中生酵母菌。在 16 项关于念珠菌病的研究中,只有 5 项能够报告抗真菌药敏试验的结果,可见诊断性试验的普及程度有限。对棘白菌素的体外抗药性非常罕见(只有 6/396 个分离株,占 1.5%)。与此相对应,氟康唑的耐药率从 0% 到 43% 不等,不同的研究和所考虑的念珠菌种类之间存在很大的异质性:我们的综述强调,巴西亟需加强监测和研究工作,以应对不断变化的念珠菌血症和抗真菌耐药性问题。尽管存在一些局限性,但现有数据表明,虽然对棘白菌素类和两性霉素 B 的耐药性仍然罕见,但念珠菌对氟康唑的耐药性却日益令人担忧。
{"title":"Systematic Review of Candidemia in Brazil: Unlocking Historical Trends and Challenges in Conducting Surveys in Middle-Income Countries.","authors":"Flávio Guinsburg Hamburger, Ana Cristina Gales, Arnaldo Lopes Colombo","doi":"10.1007/s11046-024-00867-w","DOIUrl":"10.1007/s11046-024-00867-w","url":null,"abstract":"<p><strong>Introduction: </strong>Candidemia, a bloodstream infection predominantly affecting critically ill patients, poses a significant global health threat especially with the emergence of non-albicans Candida species, including drug-resistant strains. In Brazil, limited access to advanced diagnostic tools and trained microbiologists hampers accurate identification of Candida species and susceptibility to antifungals testing hindering surveillance efforts.</p><p><strong>Methods: </strong>We conducted a systematic review spanning publications from 2017 to 2023 addressing Candida species distribution and antifungal susceptibility among Brazilian patients with candidemia.</p><p><strong>Results: </strong>Despite initially identifying 7075 records, only 16 met inclusion criteria providing accurate information of 2305 episodes of candidemia. The predominant species were C. albicans, C. parapsilosis, and C. tropicalis, followed by notable proportions of Nakaseomyces glabratus. Limited access to diagnostic tests was evident as only 5 out of 16 studies on candidemia were able to report antifungal susceptibility testing results. In vitro resistance to echinocandins was rare (only 6/396 isolates, 1,5%). In counterpart, fluconazole exhibited resistance rates ranging from 0 to 43%, with great heterogeneity among different studies and species of Candida considered.</p><p><strong>Conclusion: </strong>Our review underscores the critical need for enhanced surveillance and research efforts to address the evolving landscape of candidemia and antifungal resistance in Brazil. Despite some limitations, available data suggest that while resistance to echinocandins and amphotericin B remains rare, there is a growing concern regarding resistance to fluconazole among Candida species.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"189 4","pages":"60"},"PeriodicalIF":3.6,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Giant Panda's Skin and Hairs Infection of Nannizzia Gypsum on Lumbosacral Region Successfully Treated by Oral Terbinafine and Topic Naftifine-ketoconazole Cream. 口服特比萘芬和萘替芬-酮康唑乳膏成功治疗大熊猫腰骶部皮肤和毛发感染楠木石膏症
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-18 DOI: 10.1007/s11046-024-00843-4
Xiaoxi Xu, Junwen Huang, Li Luo, Yuping Ran
{"title":"A Giant Panda's Skin and Hairs Infection of Nannizzia Gypsum on Lumbosacral Region Successfully Treated by Oral Terbinafine and Topic Naftifine-ketoconazole Cream.","authors":"Xiaoxi Xu, Junwen Huang, Li Luo, Yuping Ran","doi":"10.1007/s11046-024-00843-4","DOIUrl":"10.1007/s11046-024-00843-4","url":null,"abstract":"","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"189 4","pages":"58"},"PeriodicalIF":3.6,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141419954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative Transcriptome Analysis of T. rubrum, T. mentagrophytes, and M. gypseum Dermatophyte Biofilms in Response to Photodynamic Therapy. 红癣菌、萌癣菌和吉普森皮真菌生物膜对光动力疗法反应的比较转录组分析。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-18 DOI: 10.1007/s11046-024-00865-y
Borui Chen, Jinyan Zhang, Juanjuan Li, Yuwen Qian, Binbin Huang, Xiaomo Wu

Dermatophyte biofilms frequently count for inadequate responses and resistance to standard antifungal treatments, resulting in refractory chronic onychomycosis infection. Although antimicrobial photodynamic therapy (aPDT) has clinically proven to exert significant antifungal effects or even capable of eradicating dermatophyte biofilms, considerably less is known about the molecular mechanisms underlying aPDT and the potential dysregulation of signaling networks that could antagonize its action. The aim of this study is to elucidate the molecular mechanisms underlining aPDT combat against dermatophyte biofilm in recalcitrant onychomycosis and to decipher the potential detoxification processes elicited by aPDT, facilitating the development of more effective photodynamic interventions. We applied genome-wide comparative transcriptome analysis to investigate how aPDT disrupting onychomycosis biofilm formed by three distinct dermatophytes, including Trichophyton rubrum, Trichophyton mentagrophytes, and Microsporum gypseum, the most frequently occurring pathogenic species. In total, 352.13 Gb of clean data were obtained for the transcriptomes of dermatophyte biofilms with or without aPDT treatment, resulting in 2,422.42 million reads with GC content of 51.84%, covering 99.9%, 98.5% and 99.4% of annotated genes of T. rubrum, T. mentagrophytes, and M. gypseum, respectively. The genome-wide orthologous analysis identified 6624 transcribed single-copy orthologous genes in all three species, and 36.5%, 6.8% and 17.9% of which were differentially expressed following aPDT treatment. Integrative orthology analysis demonstrated the upregulation of oxidoreductase activities is a highly conserved detoxification signaling alteration in response to aPDT across all investigated dermatophyte biofilms. This study provided new insights into the molecular mechanisms underneath anti-dermatophyte biofilm effects of aPDT and successfully identified a conserved detoxification regulation upon the aPDT application.

皮癣菌生物膜经常导致对标准抗真菌治疗的反应不足和耐药性,从而导致难治性慢性甲癣感染。虽然临床证明抗菌光动力疗法(aPDT)具有显著的抗真菌效果,甚至能够根除皮癣菌生物膜,但人们对 aPDT 的分子机制以及可能拮抗其作用的信号网络的潜在失调却知之甚少。本研究的目的是阐明 aPDT 对抗顽固性甲癣皮癣菌生物膜的分子机制,并破译 aPDT 可能引发的解毒过程,从而促进开发更有效的光动力干预措施。我们应用全基因组比较转录组分析,研究了 aPDT 如何破坏由三种不同的皮癣菌(包括最常见的致病菌红毛癣菌、门冬癣毛癣菌和小孢子菌)形成的甲癣生物膜。我们总共获得了 352.13 Gb 的皮癣菌生物膜转录组纯净数据,其中包括 24.2242 亿个 GC 含量为 51.84% 的读数,分别覆盖了红癣毛癣菌、门冬癣毛癣菌和小孢子菌的 99.9%、98.5% 和 99.4% 的注释基因。全基因组直向同源分析在所有三个物种中发现了 6624 个转录的单拷贝直向同源基因,其中 36.5%、6.8% 和 17.9% 的基因在 aPDT 处理后有差异表达。整合同源分析表明,氧化还原酶活性的上调是所有被研究的皮真菌生物膜对aPDT反应的一种高度保守的解毒信号改变。这项研究为了解 aPDT 抗皮真菌生物膜效应的分子机制提供了新的视角,并成功鉴定了应用 aPDT 后保守的解毒调节机制。
{"title":"Comparative Transcriptome Analysis of T. rubrum, T. mentagrophytes, and M. gypseum Dermatophyte Biofilms in Response to Photodynamic Therapy.","authors":"Borui Chen, Jinyan Zhang, Juanjuan Li, Yuwen Qian, Binbin Huang, Xiaomo Wu","doi":"10.1007/s11046-024-00865-y","DOIUrl":"10.1007/s11046-024-00865-y","url":null,"abstract":"<p><p>Dermatophyte biofilms frequently count for inadequate responses and resistance to standard antifungal treatments, resulting in refractory chronic onychomycosis infection. Although antimicrobial photodynamic therapy (aPDT) has clinically proven to exert significant antifungal effects or even capable of eradicating dermatophyte biofilms, considerably less is known about the molecular mechanisms underlying aPDT and the potential dysregulation of signaling networks that could antagonize its action. The aim of this study is to elucidate the molecular mechanisms underlining aPDT combat against dermatophyte biofilm in recalcitrant onychomycosis and to decipher the potential detoxification processes elicited by aPDT, facilitating the development of more effective photodynamic interventions. We applied genome-wide comparative transcriptome analysis to investigate how aPDT disrupting onychomycosis biofilm formed by three distinct dermatophytes, including Trichophyton rubrum, Trichophyton mentagrophytes, and Microsporum gypseum, the most frequently occurring pathogenic species. In total, 352.13 Gb of clean data were obtained for the transcriptomes of dermatophyte biofilms with or without aPDT treatment, resulting in 2,422.42 million reads with GC content of 51.84%, covering 99.9%, 98.5% and 99.4% of annotated genes of T. rubrum, T. mentagrophytes, and M. gypseum, respectively. The genome-wide orthologous analysis identified 6624 transcribed single-copy orthologous genes in all three species, and 36.5%, 6.8% and 17.9% of which were differentially expressed following aPDT treatment. Integrative orthology analysis demonstrated the upregulation of oxidoreductase activities is a highly conserved detoxification signaling alteration in response to aPDT across all investigated dermatophyte biofilms. This study provided new insights into the molecular mechanisms underneath anti-dermatophyte biofilm effects of aPDT and successfully identified a conserved detoxification regulation upon the aPDT application.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"189 4","pages":"59"},"PeriodicalIF":3.6,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141419955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aspergillus fumigatus Postoperative Fasciitis and Peritonitis. 曲霉菌术后筋膜炎和腹膜炎。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-15 DOI: 10.1007/s11046-024-00858-x
M-A Bovy, P Beckers, F Defawe, F Lifrange, J Cheret, M-P Hayette, N Layios

A 67 year-old male was admitted in the ICU because of multi-organ failure due to sepsis secondary to Fournier's gangrene. He had sustained radical prostatectomy in the last 48 hours. Peritoneal fluid and fatty tissue biopsies grew Aspergillus Fumigatus without concomitant pulmonary involvement. Postoperative acquisition via exogenous and endogenous routes is discussed, as this nosocomial entity is very rarely reported apart from peritoneal dialysis, especially in non-immunosuppressed patients.

一名 67 岁的男性因继发于福尼尔坏疽的败血症而导致多器官功能衰竭,被送入重症监护室。他在过去 48 小时内接受了前列腺根治术。腹腔积液和脂肪组织活检发现了曲霉菌,但没有同时累及肺部。本文讨论了术后通过外源性和内源性途径感染曲霉菌的情况,因为除了腹膜透析外,很少有关于这种病原菌的报道,尤其是在非免疫抑制患者中。
{"title":"Aspergillus fumigatus Postoperative Fasciitis and Peritonitis.","authors":"M-A Bovy, P Beckers, F Defawe, F Lifrange, J Cheret, M-P Hayette, N Layios","doi":"10.1007/s11046-024-00858-x","DOIUrl":"10.1007/s11046-024-00858-x","url":null,"abstract":"<p><p>A 67 year-old male was admitted in the ICU because of multi-organ failure due to sepsis secondary to Fournier's gangrene. He had sustained radical prostatectomy in the last 48 hours. Peritoneal fluid and fatty tissue biopsies grew Aspergillus Fumigatus without concomitant pulmonary involvement. Postoperative acquisition via exogenous and endogenous routes is discussed, as this nosocomial entity is very rarely reported apart from peritoneal dialysis, especially in non-immunosuppressed patients.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"189 4","pages":"57"},"PeriodicalIF":3.6,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141327696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Update on Breakthrough Invasive Mold Infections. 突破性入侵霉菌感染的最新进展。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-13 DOI: 10.1007/s11046-024-00864-z
Vera Portillo, Dionysios Neofytos

The incidence of breakthrough mold infections (bIMI) has been increasing, due to routine administration of broad-spectrum antifungal prophylaxis and an increasing pool of high-risk patient populations, with fungi more challenging to treat, resulting in a sustained high mortality, despite progress in diagnostic and therapeutic options. Pharmacokinetics of antifungal drugs, fungal, and host, including genetic, factors play a role in the emergence of bIMI. Suggested therapeutic approaches have included change of antifungal class treatment, with amphotericin-B products predominating as first-line empirical treatment and switching from one broad-spectrum azole to another remaining the most frequently used treatment modalities. Future perspectives include determining individual susceptibility to IMI to tailor prophylaxis and treatment strategies, improved diagnostic tests, and the introduction of new antifungal agents that may reduce morbidity and mortality caused by bIMI.

突破性霉菌感染(bIMI)的发病率一直在上升,原因是常规使用广谱抗真菌预防药物以及高危患者群体不断增加,尽管诊断和治疗方案取得了进展,但真菌的治疗更具挑战性,导致死亡率居高不下。抗真菌药物的药代动力学、真菌和宿主(包括遗传)因素在 bIMI 的出现中起着重要作用。建议的治疗方法包括改变抗真菌类药物的治疗方法,其中两性霉素-B 类产品作为一线经验性治疗方法占主导地位,从一种广谱唑类药物转换到另一种广谱唑类药物仍然是最常用的治疗方法。未来的展望包括:确定个人对 IMI 的易感性,以量身定制预防和治疗策略;改进诊断测试;以及引入新的抗真菌药物,以降低 bIMI 导致的发病率和死亡率。
{"title":"An Update on Breakthrough Invasive Mold Infections.","authors":"Vera Portillo, Dionysios Neofytos","doi":"10.1007/s11046-024-00864-z","DOIUrl":"10.1007/s11046-024-00864-z","url":null,"abstract":"<p><p>The incidence of breakthrough mold infections (bIMI) has been increasing, due to routine administration of broad-spectrum antifungal prophylaxis and an increasing pool of high-risk patient populations, with fungi more challenging to treat, resulting in a sustained high mortality, despite progress in diagnostic and therapeutic options. Pharmacokinetics of antifungal drugs, fungal, and host, including genetic, factors play a role in the emergence of bIMI. Suggested therapeutic approaches have included change of antifungal class treatment, with amphotericin-B products predominating as first-line empirical treatment and switching from one broad-spectrum azole to another remaining the most frequently used treatment modalities. Future perspectives include determining individual susceptibility to IMI to tailor prophylaxis and treatment strategies, improved diagnostic tests, and the introduction of new antifungal agents that may reduce morbidity and mortality caused by bIMI.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"189 4","pages":"56"},"PeriodicalIF":3.6,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11176211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141311200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Epidemiology of Tinea Capitis Among School-Children in Dschang, Western Cameroon. 喀麦隆西部 Dschang 学童头癣流行病学。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-12 DOI: 10.1007/s11046-024-00857-y
D A J Agokeng, S Dabou, J Kabtani, K B D Agokeng, K Diongue, G S S Njateng, S Ranque

Data on the epidemiology of tinea capitis (TC), an infection of the scalp by dermatophytes, are scarce in Cameroon. This study aimed to determine the prevalence of TC among school-children in the Dschang Subdivision, Western Cameroon. A cross-sectional study was carried out in June 2021 in Dschang including pupils aged 5-13. First, a standardized questionnaire was administered to participant for the collection of sociodemographic data. Then, samples were collected and cultured onto Sabouraud-Chloramphenicol-Gentamicin Agar. The etiological agents were identified based on their morphological features and with MALDI-TOF mass spectrometry. A total of 1070 children were clinically examined and 108 (10.1%) children presented with TC lesions. The mean age of the 1070 participants was 8.3 ± 2.6 years (range: 5-13 years); 772 (72.2%) were males. The use of borehole water (OR = 0.01, 95%CI[0.001-0.03]), spring water (OR = 0.2, 95%CI[0.08-0.50]), rainwater (OR = 0.004, 95%CI[0.001-0.016]), and hairdressing salons visits (OR = 0.413, 95%CI[0.196-0.872]) were associated with a decreased TC risk in the multivariate logistic regression analysis. In contrast, sharing bed with siblings (OR = 4.48, 95%CI[2.095-9.60]) was associated with an increased TC risk in children. Among the 32 dermatophytes isolated in culture, Microsporum audouinii was the most frequent (43.8%), followed by Trichophyton rubrum (25.0%) and T. soudanense (25.0%). Microsporum canis and T. violaceum were both rarely isolated. Further studies are warranted to assess the association of TC with domestic water usage that has been highlighted in this study.

头癣(TC)是一种由皮癣菌引起的头皮感染,在喀麦隆有关头癣流行病学的数据很少。本研究旨在确定喀麦隆西部Dschang分区学龄儿童中头癣的发病率。这项横断面研究于 2021 年 6 月在 Dschang 开展,包括 5-13 岁的学生。首先,对参与者进行了标准化问卷调查,以收集社会人口学数据。然后,收集样本并在沙保律-氯霉素-庆大霉素琼脂上进行培养。根据病原体的形态特征并利用 MALDI-TOF 质谱法对其进行鉴定。共对 1070 名儿童进行了临床检查,其中 108 名儿童(10.1%)出现 TC 病变。1070 名参与者的平均年龄为 8.3 ± 2.6 岁(5-13 岁),其中 772 名(72.2%)为男性。在多变量逻辑回归分析中,使用井水(OR = 0.01,95%CI[0.001-0.03])、泉水(OR = 0.2,95%CI[0.08-0.50])、雨水(OR = 0.004,95%CI[0.001-0.016])和去理发店(OR = 0.413,95%CI[0.196-0.872])与TC风险降低有关。与此相反,与兄弟姐妹同床(OR = 4.48,95%CI[2.095-9.60])与儿童 TC 风险增加有关。在培养分离出的 32 种皮癣菌中,奥杜茵小孢子菌最常见(43.8%),其次是红毛癣菌(25.0%)和苏旦癣菌(25.0%)。犬小孢子菌和小毛癣菌很少被分离出来。有必要进行进一步研究,以评估本研究中强调的癣菌与家庭用水的关系。
{"title":"Epidemiology of Tinea Capitis Among School-Children in Dschang, Western Cameroon.","authors":"D A J Agokeng, S Dabou, J Kabtani, K B D Agokeng, K Diongue, G S S Njateng, S Ranque","doi":"10.1007/s11046-024-00857-y","DOIUrl":"10.1007/s11046-024-00857-y","url":null,"abstract":"<p><p>Data on the epidemiology of tinea capitis (TC), an infection of the scalp by dermatophytes, are scarce in Cameroon. This study aimed to determine the prevalence of TC among school-children in the Dschang Subdivision, Western Cameroon. A cross-sectional study was carried out in June 2021 in Dschang including pupils aged 5-13. First, a standardized questionnaire was administered to participant for the collection of sociodemographic data. Then, samples were collected and cultured onto Sabouraud-Chloramphenicol-Gentamicin Agar. The etiological agents were identified based on their morphological features and with MALDI-TOF mass spectrometry. A total of 1070 children were clinically examined and 108 (10.1%) children presented with TC lesions. The mean age of the 1070 participants was 8.3 ± 2.6 years (range: 5-13 years); 772 (72.2%) were males. The use of borehole water (OR = 0.01, <sub>95%</sub>CI[0.001-0.03]), spring water (OR = 0.2, <sub>95%</sub>CI[0.08-0.50]), rainwater (OR = 0.004, <sub>95%</sub>CI[0.001-0.016]), and hairdressing salons visits (OR = 0.413, <sub>95%</sub>CI[0.196-0.872]) were associated with a decreased TC risk in the multivariate logistic regression analysis. In contrast, sharing bed with siblings (OR = 4.48, <sub>95%</sub>CI[2.095-9.60]) was associated with an increased TC risk in children. Among the 32 dermatophytes isolated in culture, Microsporum audouinii was the most frequent (43.8%), followed by Trichophyton rubrum (25.0%) and T. soudanense (25.0%). Microsporum canis and T. violaceum were both rarely isolated. Further studies are warranted to assess the association of TC with domestic water usage that has been highlighted in this study.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"189 4","pages":"51"},"PeriodicalIF":3.6,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multifocal Sporotrichosis Associated with Armadillo Hunting in Midwest Brazil: An In-Depth Case Study and Comprehensive Literature Analysis. 巴西中西部地区与犰狳狩猎有关的多灶性孢子丝虫病:一项深入的案例研究和全面的文献分析。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-12 DOI: 10.1007/s11046-024-00854-1
Anderson Messias Rodrigues, Jamile Ambrósio de Carvalho, Andreia Ferreira Nery, Marcia Hueb, Ivana Garcia, Armando Guevara, Zoilo Pires de Camargo, Rosane Christine Hahn

Sporotrichosis is a globally distributed subcutaneous mycosis caused by dimorphic Sporothrix species commonly found in soil, mosses, and decaying plant matter. The lymphocutaneous manifestation, historically associated with occupational activities and sapronotic transmission, has recently been observed to also occur through animal contact, particularly notable in Brazil. We describe a rare case of lymphocutaneous sporotrichosis with simultaneous pulmonary complications resulting from the scratching of a southern three-banded armadillo, Tolypeutes matacus, primarily inhabiting the arid forests of South America's central region. Speciation using multiplex quantitative polymerase chain reaction (qPCR) established the etiological agent as S. schenckii s. str., while amplified fragment length polymorphism (AFLP) analysis unveiled a novel genotype circulating in the Midwest of Brazil. The patient received treatment with itraconazole (200 mg/day) for two months, leading to substantial clinical improvement of cutaneous and pulmonary symptoms. This case highlights the critical role of animal-mediated transmission in sporotrichosis epidemiology, particularly within regions with diverse armadillo species. The unusual epidemiology and genetic characteristics of this case emphasize the need for enhanced awareness and diagnostic vigilance in atypical sporotrichosis presentations.

孢子丝菌病是一种分布于全球的皮下真菌病,由常见于土壤、苔藓和腐烂植物中的二形孢子丝菌引起。淋巴皮肤表现历来与职业活动和无菌传播有关,但最近观察到也可通过动物接触发生,这在巴西尤为显著。我们描述了一例罕见的淋巴皮肤孢子丝菌病病例,该病例因搔抓主要栖息于南美洲中部干旱森林的南方三带犰狳(Tolypeutes matacus)而引起,同时伴有肺部并发症。利用多重定量聚合酶链反应(qPCR)进行的物种鉴定确定了病原体为S. schenckii s. str.,而扩增片段长度多态性(AFLP)分析则揭示了一种在巴西中西部地区流行的新型基因型。患者接受了为期两个月的伊曲康唑(200 毫克/天)治疗,皮肤和肺部症状得到了显著改善。该病例凸显了动物媒介传播在孢子丝菌病流行病学中的关键作用,尤其是在犰狳种类繁多的地区。该病例不寻常的流行病学和遗传特征强调了在非典型孢子丝菌病病例中提高意识和诊断警惕的必要性。
{"title":"Multifocal Sporotrichosis Associated with Armadillo Hunting in Midwest Brazil: An In-Depth Case Study and Comprehensive Literature Analysis.","authors":"Anderson Messias Rodrigues, Jamile Ambrósio de Carvalho, Andreia Ferreira Nery, Marcia Hueb, Ivana Garcia, Armando Guevara, Zoilo Pires de Camargo, Rosane Christine Hahn","doi":"10.1007/s11046-024-00854-1","DOIUrl":"10.1007/s11046-024-00854-1","url":null,"abstract":"<p><p>Sporotrichosis is a globally distributed subcutaneous mycosis caused by dimorphic Sporothrix species commonly found in soil, mosses, and decaying plant matter. The lymphocutaneous manifestation, historically associated with occupational activities and sapronotic transmission, has recently been observed to also occur through animal contact, particularly notable in Brazil. We describe a rare case of lymphocutaneous sporotrichosis with simultaneous pulmonary complications resulting from the scratching of a southern three-banded armadillo, Tolypeutes matacus, primarily inhabiting the arid forests of South America's central region. Speciation using multiplex quantitative polymerase chain reaction (qPCR) established the etiological agent as S. schenckii s. str., while amplified fragment length polymorphism (AFLP) analysis unveiled a novel genotype circulating in the Midwest of Brazil. The patient received treatment with itraconazole (200 mg/day) for two months, leading to substantial clinical improvement of cutaneous and pulmonary symptoms. This case highlights the critical role of animal-mediated transmission in sporotrichosis epidemiology, particularly within regions with diverse armadillo species. The unusual epidemiology and genetic characteristics of this case emphasize the need for enhanced awareness and diagnostic vigilance in atypical sporotrichosis presentations.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"189 4","pages":"53"},"PeriodicalIF":3.6,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rethinking Aspergillosis in the Era of Microbiota and Mycobiota. 在微生物群和霉菌生物群时代重新思考曲霉菌病。
IF 3.6 3区 生物学 Q2 MYCOLOGY Pub Date : 2024-06-12 DOI: 10.1007/s11046-024-00853-2
Aleksandra Barac, Ankica Vujovic, Jovan Peric, Ivan Tulic, Maja Stojanovic, Mihailo Stjepanovic

Aspergillosis encompasses a wide range of clinical conditions based on the interaction between Aspergillus and the host. It ranges from colonization to invasive aspergillosis. The human lung provides an entry door for Aspergillus. Aspergillus has virulence characteristics such as conidia, rapid growth at body temperature, and the production of specific proteins, carbohydrates, and secondary metabolites that allow A. fumigatus to infiltrate the lung's alveoli and cause invasive aspergillosis. Alveolar epithelial cells play an important role in both fungus clearance and immune cell recruitment via cytokine release. Although the innate immune system quickly clears conidia in immunocompetent hosts, A. fumigatus has evolved multiple virulence factors in order to escape immune response such as ROS detoxifying enzymes, the rodlet layer, DHN-melanin and toxins. Bacterial co-infections or interactions can alter the immune response, impact Aspergillus growth and virulence, enhance biofilm formation, confound diagnosis, and reduce treatment efficacy. The gut microbiome's makeup influences pulmonary immune responses generated by A. fumigatus infection and vice versa. The real-time PCR for Aspergillus DNA detection might be a particularly useful tool to diagnose pulmonary aspergillosis. Metagenomics analyses allow quick and easy detection and identification of a great variety of fungi in different clinical samples, although optimization is still required particularly for the use of NGS techniques. This review will analyze the current state of aspergillosis in light of recent discoveries in the microbiota and mycobiota.

曲霉菌病包括各种基于曲霉菌与宿主相互作用的临床症状。它包括从定植到侵袭性曲霉菌病。人的肺部是曲霉菌的入口。曲霉菌具有分生孢子、在体温下快速生长以及产生特定蛋白质、碳水化合物和次生代谢物等毒力特征,这些特征使得烟曲霉菌能够渗入肺泡并引起侵袭性曲霉菌病。肺泡上皮细胞在清除真菌和通过释放细胞因子招募免疫细胞方面都发挥着重要作用。虽然先天性免疫系统能迅速清除免疫功能正常宿主的分生孢子,但烟曲霉已进化出多种毒力因子,以逃避免疫反应,如 ROS 解毒酶、小杆层、DHN-黑色素和毒素。细菌合并感染或相互作用会改变免疫反应,影响曲霉菌的生长和毒力,促进生物膜的形成,混淆诊断并降低治疗效果。肠道微生物组的构成会影响烟曲霉感染产生的肺部免疫反应,反之亦然。用于检测曲霉菌 DNA 的实时 PCR 可能是诊断肺曲霉菌病的一种特别有用的工具。元基因组学分析可快速、简便地检测和鉴定不同临床样本中的多种真菌,但仍需优化,特别是在使用 NGS 技术时。本综述将根据微生物群和真菌生物群的最新发现分析曲霉菌病的现状。
{"title":"Rethinking Aspergillosis in the Era of Microbiota and Mycobiota.","authors":"Aleksandra Barac, Ankica Vujovic, Jovan Peric, Ivan Tulic, Maja Stojanovic, Mihailo Stjepanovic","doi":"10.1007/s11046-024-00853-2","DOIUrl":"10.1007/s11046-024-00853-2","url":null,"abstract":"<p><p>Aspergillosis encompasses a wide range of clinical conditions based on the interaction between Aspergillus and the host. It ranges from colonization to invasive aspergillosis. The human lung provides an entry door for Aspergillus. Aspergillus has virulence characteristics such as conidia, rapid growth at body temperature, and the production of specific proteins, carbohydrates, and secondary metabolites that allow A. fumigatus to infiltrate the lung's alveoli and cause invasive aspergillosis. Alveolar epithelial cells play an important role in both fungus clearance and immune cell recruitment via cytokine release. Although the innate immune system quickly clears conidia in immunocompetent hosts, A. fumigatus has evolved multiple virulence factors in order to escape immune response such as ROS detoxifying enzymes, the rodlet layer, DHN-melanin and toxins. Bacterial co-infections or interactions can alter the immune response, impact Aspergillus growth and virulence, enhance biofilm formation, confound diagnosis, and reduce treatment efficacy. The gut microbiome's makeup influences pulmonary immune responses generated by A. fumigatus infection and vice versa. The real-time PCR for Aspergillus DNA detection might be a particularly useful tool to diagnose pulmonary aspergillosis. Metagenomics analyses allow quick and easy detection and identification of a great variety of fungi in different clinical samples, although optimization is still required particularly for the use of NGS techniques. This review will analyze the current state of aspergillosis in light of recent discoveries in the microbiota and mycobiota.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"189 4","pages":"49"},"PeriodicalIF":3.6,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Mycopathologia
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1