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Depressive Symptoms and Risk of Occupational and Non-occupational Injury inthe US Working Population 美国工作人群的抑郁症状与职业和非职业伤害风险
Pub Date : 2015-05-30 DOI: 10.4172/2329-6879.1000200
Jaeyoung Kim
The purpose of this study was to examine the association between depressive symptoms and the subsequent incidence of injury by its work-relatedness in the US working population. This study analysed pooled panel data from the Medical Expenditure Panel Survey (MEPS) for 31,138 workers, aged 18 to 64 years, during the years 2000-06. Depressive symptoms were assessed using the EuroQoL (EQ-5D), a general mental distress scale (K-6) and Patients Health Questionnaire 2 item depression screener (PHQ-2). Injuries were identified from the medical conditions captured in personal interviews and coded using the ICD-9 by coders trained in the MEPS. A discrete time proportional odds model was used to calculate the relative risks. A total of 7.9% of workers had depressive symptoms at the baseline. Among workers with depressive symptoms at baseline, 13.1% reported a nonoccupational injury, 6.2% reported an occupational injury during follow-up. Workers with depressive symptoms are at an increased, but a similar risk of both occupational and non-occupational injury. For workers treated with antidepressants, the analysis did not reveal a different pattern of association with either type of injury occurrence. This association between depressive symptoms and injury diminished over time. Earlier intervention for workers with depressive symptoms in the workplace could be beneficial for preventing injuries.
本研究的目的是在美国工作人群中检查抑郁症状与随后的工伤发生率之间的关系。本研究分析了2000- 2006年期间医疗支出小组调查(MEPS)中31,138名年龄在18至64岁的工人的汇总小组数据。采用EuroQoL (EQ-5D)、一般精神痛苦量表(K-6)和患者健康问卷2项抑郁筛查(PHQ-2)对抑郁症状进行评估。从个人访谈中记录的医疗状况中确定受伤情况,并由受过MEPS培训的编码员使用ICD-9进行编码。采用离散时间比例赔率模型计算相对风险。在基线时,共有7.9%的工人有抑郁症状。在基线时有抑郁症状的工人中,13.1%报告了非职业伤害,6.2%报告了随访期间的职业伤害。有抑郁症状的工人发生职业和非职业伤害的风险增加,但相似。对于接受抗抑郁药物治疗的工人,分析并没有揭示出与任何一种伤害发生的不同模式的关联。随着时间的推移,抑郁症状和损伤之间的联系逐渐减弱。在工作场所对有抑郁症状的工人进行早期干预可能有利于防止受伤。
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引用次数: 1
Frequency, Intensity, Time And Type Of Tasks Performed During Wildfire Suppression 在扑灭野火期间执行的任务的频率、强度、时间和类型
Pub Date : 2015-04-28 DOI: 10.4172/2329-6879.1000199
M. Phillips, K. Netto, W. Payne, D. Nichols, Cara Lord, Neil Brooksbank, B. Aisbett
Objective: To quantify the frequency, intensity, duration, and type of tasks performed by Australian rural fire crews when suppressing wildfires. Methods: Twenty-eight Australian rural firefighters worked across four, six-hour shifts fighting to curtail the spread of wildfire. Each firefighter wore a heart rate monitor and personal global positioning system (GPS) unit and was followed by a researcher filming their work activity. Video footage of each firefighter was synchronized with their heart rate and GPS data to quantify the frequency, intensity and duration of individual fireground tasks. Fireground tasks were isolated using a previously conducted job task analysis. Results: Firefighters performed 32 distinct fireground tasks. Task frequencies ranged from once to 103 times per six-hour shift. Individual tasks lasted 4 ± 2 s to 461 ± 387 s, were performed at speeds ranging from 0.12 ± 0.08 m•s-1 to 0.79 ± 0.40 m•s-1 and elicited mean heart rates that ranged between 97 ± 16 beats•min-1 (55.7 ± 8.7 percentage of age-predicted maximum heart rate (HRmax)) and 157 ± 15 beats•min-1 (86.2 ± 10.8%HRmax). Conclusion: Fireground tasks were, generally speaking, shorter, slower, and elicited lower heart rates than equivalent tasks previously simulated and reported in the literature. The differences between naturally occurring and simulated tasks question the value of isolated task simulations for conducting physical demands analyses en-route to developing job-specific fitness tests.
目的:量化澳大利亚农村消防人员在扑灭野火时所执行的任务的频率、强度、持续时间和类型。方法:28名澳大利亚农村消防员每班工作4、6小时,以遏制野火的蔓延。每位消防员都佩戴心率监测器和个人全球定位系统(GPS)装置,并由一名研究人员跟踪拍摄他们的工作活动。每个消防员的视频片段与他们的心率和GPS数据同步,以量化个人消防任务的频率、强度和持续时间。使用先前进行的工作任务分析来隔离火场任务。结果:消防员执行了32项不同的火场任务。任务频率从每6小时轮班一次到103次不等。单个任务持续4±2秒至461±387秒,速度范围为0.12±0.08 m•s-1至0.79±0.40 m•s-1,平均心率范围为97±16次•min-1(占年龄预测最大心率(HRmax)的55.7±8.7 %)和157±15次•min-1(占年龄预测最大心率(HRmax)的86.2±10.8%)。结论:一般来说,火场任务比先前模拟和文献报道的同等任务更短,更慢,引起的心率更低。自然发生的任务和模拟任务之间的差异质疑了孤立任务模拟在制定特定工作体能测试的过程中进行身体需求分析的价值。
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引用次数: 11
Oxygen Uptake and Heart Rate during Simulated Wildfire Suppression Tasks Performed by Australian Rural Firefighters 澳大利亚农村消防员在模拟野火灭火任务中的摄氧量和心率
Pub Date : 2015-04-28 DOI: 10.4172/2329-6879.1000198
M. Phillips, W. Payne, K. Netto, Shane Cramer, D. Nichols, G. McConell, Cara Lord, B. Aisbett
Objective: Australian rural fire crews safeguard the nation against the annual devastation of wildfire. We have previously reported that experienced firefighters identified seven physically demanding tasks for Australian rural fire crews suppressing wildfires. These firefighters rated the operational importance, typical duration, core fitness components, and likely frequency of the seven tasks. The intensity of these duties remains unknown. The aim of this study was to quantify the oxygen uptake (VO2), heart rate (HR) and movement speed responses during simulations of these physically demanding wildfire suppression tasks. Method: Twenty six rural firefighters (20 men, six women) performed up to seven tasks, during which time their HR and movement speed were recorded. The VO2 for each task was also calculated from the analysis of expired air collected in Douglas bags. Firefighters’ HR and movement speed were measured using HR monitors and portable global positioning system units, respectively. Results: The hose work tasks elicited a VO2 of 21-27 mL·kg-1·min-1 and peak HR of 77-87% age-predicted maximal HR (HRmax). Hand tool tasks were accompanied by VO2 of 28-34 mL·kg-1·min-1 and peak HR of 85-95%HRmax. Firefighters’ movement speed spanned 0.2 ± 0.1 to 1.8 ± 0.2 m·s-1 across the seven tasks. The cardiovascular responses in the hand tool tasks were, in most cases, higher (P<0.05) than during those elicited by the hose work tasks. Conclusions: The cardiovascular responses elicited during simulations of physically demanding wildfire suppression approximated those reported for similar tasks in urban and forestry fire fighting jurisdictions. The findings may prompt Australian rural fire agencies to consider cardiovascular disease risk screening and physical selection testing to ensure that healthy and fit firefighters are deployed to the fire ground.
目的:澳大利亚农村消防队员保护国家免受每年野火的破坏。我们之前报道过,经验丰富的消防员确定了澳大利亚农村消防员扑灭野火的七项体力要求高的任务。这些消防员对这七项任务的操作重要性、典型持续时间、核心健身成分和可能出现的频率进行了评分。这些关税的强度仍不得而知。本研究的目的是量化在模拟这些体力要求很高的野火灭火任务时的摄氧量(VO2)、心率(HR)和运动速度反应。方法:26名农村消防员(20名男性,6名女性)执行多达7项任务,在此期间记录他们的HR和移动速度。通过分析道格拉斯袋中收集的过期空气,还计算了每个任务的VO2。消防员的心率和移动速度分别用心率监测仪和便携式全球定位系统测量。结果:这些工作任务引起的VO2为21 ~ 27 mL·kg-1·min-1,峰值HR为77 ~ 87%的年龄预测最大HR (HRmax)。手工工具作业的VO2为28 ~ 34 mL·kg-1·min-1,峰值HR为85 ~ 95% hrmax。在7个任务中,消防员的移动速度范围为0.2±0.1 ~ 1.8±0.2 m·s-1。在大多数情况下,手工工具任务的心血管反应高于软管任务的心血管反应(P<0.05)。结论:在模拟对体力要求很高的野火扑灭过程中引发的心血管反应与在城市和森林消防辖区中报道的类似任务相似。这一发现可能会促使澳大利亚农村消防机构考虑心血管疾病风险筛查和身体选择测试,以确保将健康和适合的消防员部署到火场。
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引用次数: 9
Magnitude of Occupational Injuries and Associated Factors among Small-Scale Industry Workers in Mekelle City, Northern Ethiopia 埃塞俄比亚北部Mekelle市小型工业工人职业伤害程度及相关因素
Pub Date : 2015-04-13 DOI: 10.4172/2329-6879.1000197
A. Berhe, Dejen Yemane, Azeb Gebresilassie, Wendwossen Terefe, Lalit T. Ingale
Background: Occupational injuries are important public health problems that comprise a major part of injury burden in Ethiopia. The present study investigates the magnitude and associated factors with occupational injuries among small-scale industry workers in Mekelle city, Northern Ethiopia in 2013. Methods: A cross-sectional study was conducted on 774 small-scale industry workers from March to April 2013. Multi-stage sampling technique was used to select the study participant’s. A pre-tested and structured questionnaire was used to obtain information on the occurrence of occupational injuries and allied factors. Data was analyzed using STATA software version 12. Bi-variate and multivariate analysis was carried out to ascertain the association between dependent and independent variables. Results: A total of 758 (97.9%) small-scale industry workers were interviewed. A one-year prevalence rate of at least one occupational injury among the small-scale industry workers was 58.2%. Use of personal protective equipments [AOR=3.43, 95%CI: 2.39-4.94], age of respondent’s [AOR=1.51, 95%CI: 1.02 - 2.25], number of years worked in the same job [AOR=2.89, 95%CI: 1.88-4.43], number of hours worked per week [AOR=2.73, 95%CI: 1.92- 3.87], and the job category; metalworkers [AOR=3.17, 95%CI: 2.07-4.85] and wood workers [AOR=2.34, 95%CI: 1.39-3.92] were found to be significantly associated factors with occupational injury among small-scale industry workers. Conclusion: This study concludes that the prevalence of occupational injuries among small-scale industry workers was considerably high. Hence, promoting occupational safety and health through appropriate prevention programs and provision of comprehensive occupational health and safety services with the provisions of personal protective devices, and focused interventions for young, less experienced, workers who work for extended hours (>48 hours per week) and workers working on metal and wood works are highly advised.
背景:职业伤害是重要的公共卫生问题,构成埃塞俄比亚伤害负担的主要部分。本研究调查了2013年埃塞俄比亚北部Mekelle市小规模工业工人的职业伤害程度和相关因素。方法:2013年3 - 4月对774名小规模产业工人进行横断面调查。采用多阶段抽样技术选择研究对象。一份预先测试和结构化的问卷被用来获得关于职业伤害发生和相关因素的信息。数据分析采用STATA软件12版。进行双变量和多变量分析以确定因变量和自变量之间的关联。结果:共访谈小规模产业工人758人(97.9%)。小规模工业工人一年至少有一次职业伤害的患病率为58.2%。个人防护装备使用情况[AOR=3.43, 95%CI: 2.39 ~ 4.94]、年龄[AOR=1.51, 95%CI: 1.02 ~ 2.25]、同一岗位工作年限[AOR=2.89, 95%CI: 1.88 ~ 4.43]、每周工作时数[AOR=2.73, 95%CI: 1.92 ~ 3.87]、工作类别;金属工人[AOR=3.17, 95%CI: 2.07-4.85]和木工工人[AOR=2.34, 95%CI: 1.39-3.92]是小作坊工人职业伤害的显著相关因素。结论:本研究认为,小作坊工人的职业伤害发生率相当高。因此,强烈建议通过适当的预防规划和提供全面的职业健康与安全服务,并提供个人防护装置,促进职业安全与健康,并针对年轻、经验不足、工作时间延长(每周超过48小时)的工人以及从事金属和木材工作的工人采取重点干预措施。
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引用次数: 29
HIV /AIDS and the Oral Manifestations Associated with the Disease 艾滋病毒/艾滋病和与该疾病相关的口腔表现
Pub Date : 2015-03-27 DOI: 10.4172/2329-6879.1000E108
M. Cecaro, K. Naidu
HIV/AIDS is considered one of the world’s largest pandemics affecting millions of people across the globe. For the latter part of the twenty century, HIV/AIDS has dominated much of the headlines with regard to media attention owing to the devastating consequences that it inflicts to those infected with this dreadful disease. It has attracted both international medical and political attention. It has a great impact on society both as an illness, that of discrimination and economic impact. Hence it’s always been the focus of mass attention particularly the media. The media plays a large role with regard to providing the most valuable, up to date and informative healthcare information regarding this pandemic. This ensures that the public is well informed, educated but above all a public that is armed with the necessary knowledge to counter act this growing pandemic. An important aspect surrounding public information is proper information handed to them by the relevant healthcare professionals. The aim of this short informative editorial is to focus on oral manifestations of HIV/AIDS. It is hoped that valuable information can be gathered by the public regarding these important clinical manifestations and in doing so assist them with identifying some of the signs and symptoms that will prompt them to seek help.
艾滋病毒/艾滋病被认为是影响全球数百万人的世界上最大的流行病之一。20世纪后半叶,由于艾滋病毒/艾滋病给感染这一可怕疾病的人造成毁灭性后果,艾滋病毒/艾滋病占据了媒体关注的大部分头条新闻。它引起了国际医学界和政界的关注。它对社会有很大的影响,作为一种疾病,歧视和经济影响。因此,它一直是大众关注的焦点,尤其是媒体。媒体在提供有关这一流行病的最有价值、最新和最翔实的卫生保健信息方面发挥着重要作用。这确保公众得到充分的了解和教育,但最重要的是使公众掌握必要的知识,以应对这一日益严重的流行病。公共信息的一个重要方面是由相关医疗保健专业人员向他们提供适当的信息。这篇简短的信息社论的目的是关注艾滋病毒/艾滋病的口腔表现。希望公众能够收集到有关这些重要临床表现的宝贵信息,从而帮助他们识别一些促使他们寻求帮助的体征和症状。
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引用次数: 0
Influence of Vascular Occlusion in Concentration of Growth Hormone andLactate in Athletes during Strengthening Quadriceps Exercise 血管闭塞对四头肌强化训练中运动员生长激素和乳酸浓度的影响
Pub Date : 2015-03-27 DOI: 10.4172/2329-6879.1000195
S. N. Leite, A. C. Reis, Gustavo Lacreta Toledo Colnezi, F. H. Souza, Haroldo Fernando Silva Ferracini, P. Lucareli, S. S. Lodovichi
Objective: The aim of this comparative experimental study designed is analyze the possible changes in GH serum and lactate during the performance of resistance exercise-induced ischemia in young athletes. A possible outcome will be to promote greater strength gains and muscle hypertrophy during the training protocol. Design: This survey was conducted with 18 male football players 18 years of age. The athletes were divided into two groups; one group was subjected only to quadriceps exercises; the other group was subjected to quadriceps exercises associated with vascular occlusion. Levels of lactate and GH were observed before and after resistance exercise. Results: Comparing intergroup data, there were no statistically significant results. However, when comparing data within the groups, there was an increase in Group 2 obtained for both the lactate threshold and GH. Group 1 showed an increase in the lactate threshold, but a decrease in GH. Conclusions: This new method of resistance training should be analyzed, and further experiments are needed to optimize the protocol to reach optimum high intensity resistance training in young athletes.
目的:本对比实验研究旨在分析青年运动员在抗阻性运动引起的缺血过程中GH血清和乳酸的可能变化。一个可能的结果是在训练方案中促进更大的力量增加和肌肉肥大。设计:本调查以18名18岁的男性足球运动员为研究对象。运动员被分成两组;一组只进行股四头肌锻炼;另一组进行与血管闭塞相关的股四头肌运动。在抗阻运动前后观察乳酸和生长激素水平。结果:组间资料比较,差异无统计学意义。然而,当比较组内数据时,第2组的乳酸阈值和生长激素均有所增加。第1组乳酸阈值升高,生长激素降低。结论:对这种新的抗阻训练方法进行分析,并需要进一步的实验来优化方案,以达到青年运动员最佳的高强度抗阻训练。
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引用次数: 5
Health Warning Preparedness, Surveillance and a New Understanding may be Our Solution to Multi-drug Resistant Organisms 健康预警准备、监测和对多重耐药菌的新认识可能是我们的解决方案
Pub Date : 2015-03-09 DOI: 10.4172/2329-6879.1000E107
Cecaro Massimo, Yog, ree Ramsamy
In reality, humans do need bacteria to carry out normal physiological functions in addition serve as a protective barrier against colonization with more pathogenic and virulent strains of organisms. The human host forms a niche and provides nutrition for colonizing microbes. The microbe in turn occupies a space that a potential parasite or pathogen may otherwise invade. This relationship that has been in existence since the begging of man has been essentially mutualistic until now....
在现实中,人类确实需要细菌来执行正常的生理功能,并作为一种保护屏障,防止更多致病性和毒性菌株的生物定植。人类宿主形成一个生态位,为定植的微生物提供营养。反过来,微生物占据了潜在的寄生虫或病原体可能入侵的空间。这种关系自人类乞讨以来一直存在,直到现在基本上是互惠互利的....
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引用次数: 0
Effect of Manual Force on Lumbar Side Bending Range of Motion 人手对腰侧弯曲活动范围的影响
Pub Date : 2015-02-17 DOI: 10.4172/2329-6879.1000194
Tomonori Sato
Objective: We evaluated the immediate changes in intervertebral motion after application of lumbar manual force. Methods: Fifteen male volunteers (aged 26–43 years) with no history of significant low back pain were recruited to participate as a study group (manual traction). Control group (without manual traction) consisted of fifteen male volunteers who are matched with age (age 21-45 years). Consenting volunteers were referred for three radiographs (neutral position, right side bending position prior to manual force and right side bending position after manual force). The L3/4 segment was chosen for manual force, which was performed by a single examiner. Frontal angular rotation of each lumbar spine segment and total lumbar spinal motion were measured radio graphically before and after manual force by a single investigator (radiologist). Within-group differences were assessed with the paired t test. Results: Analysis of the pre-and post-manual force radiographs showed a significant average increase at the L3/4 segment from 4.9° to 6.4° and an improvement in the total range of motion from 17.8° to 19.5° in the study group. No significant increase was found at the L1/2, L2/3, or L4/5 segments in the study group. In the control group, there were no significant changes in all measurements. Conclusion: These results suggest that manual force targeting the L3/4 segment results in an immediate increase in the angular motion of the L3/4 segment and improvement in total motion of the lumbar spine.
目的:评估腰椎手压后椎间运动的即时变化。方法:招募15名无明显腰痛史的男性志愿者(26-43岁)作为研究组(手动牵引)。对照组(无手动牵引)由15名年龄匹配的男性志愿者组成(21-45岁)。同意的志愿者被推荐拍摄三张x线片(中立位,手动前的右侧弯曲位和手动后的右侧弯曲位)。选择L3/4节段进行手动用力,由一名审查员完成。每个腰椎节段的前角旋转和腰椎的总运动由一名研究者(放射科医生)在用力前后进行放射成像测量。采用配对t检验评估组内差异。结果:手工前后的x线片分析显示,研究组的L3/4节段平均从4.9°增加到6.4°,总活动范围从17.8°增加到19.5°。在研究组中,L1/2、L2/3或L4/5节段未发现明显增加。在对照组中,所有测量结果均无显著变化。结论:这些结果表明,针对L3/4节段的手动力可立即增加L3/4节段的角运动并改善腰椎的总运动。
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引用次数: 0
Camel Bites - A Unique Experience 骆驼咬伤-一个独特的经验
Pub Date : 2015-01-26 DOI: 10.4172/2329-6879.1000192
D. Sharma, S. Devgarha, R. Mathur
Introduction: Camel bites injuries are occupational and seasonal hazards which can cause serious limb, neck and chest injuries, potentially threatening the vascularity of the limbs with skin and soft tissue injuries. Proper health education of the people involved in handling camels and precautions and protective mask to camel's mouth during late winter and early summer can avoid these injuries. Proper referral, urgent revascularization can restore the vascularity of limb and prevent limb loss. Material and methods: We from the Department of CTVS, Sawai Mansingh Medical College and Hospitals, Jaipur, Rajasthan, India carried out a study of all the camel bite cases those presented to us or were referred to us for vascular and thoracic injuries, the factors that were responsible for the bite, its treatment and consequences were fully analyzed and studied. Observations: Between August 2008 to December 2013, 31 cases of Camel bite were encountered, out of which 23 cases were arterial injuries and 6 were thoracic wall bites and 2 were neck injuries. All were caused by domesticated camels, involved dominant side upper limbs/neck in 17 out of 23 arterial injury cases, dominant side of chest wall in 4 out of 6 thoracic wall bites, all bites were unprovoked, occurred during work or feeding, seasonally most occurred between december to march (during late winter and early summer) which can be co-related to mating season of camels. Results: 100% limb salvage was achieved with end to end repair in 13 cases and reverse saphenous vein interposition graft in 10 cases, with trunk flap in 2 and latissimus dorsi flap in 1 to give soft tissue cover. One case of Right axillary artery injury was encountered which was treated with subclavian to brachial bypass. 4 thoracic wall bites had hemothorax which were treated with intercostal tube drainage.
骆驼咬伤是一种职业性和季节性危害,可造成严重的肢体、颈部和胸部损伤,潜在地威胁到皮肤和软组织损伤的肢体血管。对处理骆驼的人员进行适当的健康教育,并在冬末夏初给骆驼戴上防护口罩,可以避免这些伤害。适当的转诊、紧急的血运重建术可以恢复肢体的血运,防止肢体丧失。材料和方法:我们来自印度拉贾斯坦邦斋浦尔Sawai Mansingh医学院和医院cvs部门,对所有因血管和胸部损伤而向我们提交或转诊的骆驼咬伤病例进行了研究,对咬伤的原因、治疗方法和后果进行了充分的分析和研究。观察:2008年8月至2013年12月共收治骆驼咬伤31例,其中动脉损伤23例,胸壁咬伤6例,颈部损伤2例。23例动脉损伤中17例累及主侧上肢/颈部,6例胸壁咬伤中4例累及主侧胸壁,均为无端咬伤,发生于工作或进食期间,季节性多发生于12月至3月(冬末夏初),与骆驼的交配季节有关。结果:端对端修复13例,反向隐静脉间置移植10例,残肢100%保留,主干瓣2例,背阔肌瓣1例,给予软组织覆盖。本文报道1例右腋窝动脉损伤,采用锁骨下至肱动脉搭桥术治疗。4例胸壁咬伤并发血胸,行肋间管引流治疗。
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引用次数: 3
Effects of Pyriproxyfen on Viability and Increase of Intracellular Lipids in HepG2 Cell Line 吡丙醚对HepG2细胞活力及细胞内脂质的影响
Pub Date : 2014-12-18 DOI: 10.4172/2329-6879.1000189
M. Lamberti, A. Stellavato, A. Pirozzi, A. D'Agostino, G. Panariello, N. Sannolo, C. Schiraldi
Abstract Introduction: Pyriproxyfen, (2-[1-methyl-2-(4-phenoxyphenoxy) ethoxy] pyridine) (PPF) is an insecticidal used in household, agricultural, and horticultural applications to control many insect species. We tested its hepatic toxicity in hepatoma HepG2 cell line, we also evaluate if PPF could induce nonalcoholic fatty liver disease. Materials and methods: The hepatoma HepG2 cell line was exposed for 24-48 hrs with serum-free DMEM to the active principles at different concentrations. The cell viability was assessed by measuring reduction of the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). For the evaluation of in vitro steatosis, the cells were rinsed with cold phosphate buffered saline (PBS) and fixed in 4% paraformaldehyde. Images of cell were captured using an optic microscope and stained lipid droplets were then extracted with isopropanol (60%) for quantification by measuring its absorbance at 510 nm. Results: The MTT-test showed that PPF is cytotoxic at all concentrations tested both at 24 h and 48 h. Cell viability is below 50% for concentrations 1-10 ppm while the viability is less than 10% for the concentration 100 ppm. PPF induces the increasing intracellular lipids from 1 ppm concentration. The maximum effect is observed at 100 ppm. Discussion: In our in vitro study we found a loss of cell viability of about 50% for concentrations from 1-10 ppm by the MTT-Test that measures mitochondrial enzyme activity. Because the mitochondrial enzyme activity affected major changes at the starting/beginning of the apoptotic this condition suggested that PPF is strongly cytotoxic to human hepatocytes in the presented assays. Already at 1 ppm concentration PPF induces the increasing intracellular lipids, in HepG2 in vitro culture.
摘要简介:Pyriproxyfen,(2-[1-甲基-2-(4-phenoxyphenoxy)乙氧基]pyridine) (PPF)是一种用于家庭、农业和园艺的杀虫剂,用于控制多种昆虫。我们在肝癌HepG2细胞系中测试了它的肝毒性,我们也评估了PPF是否能诱导非酒精性脂肪性肝病。材料与方法:用无血清DMEM将肝癌HepG2细胞株暴露于不同浓度的活性成分中24-48小时。通过测定四氮唑染料3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑(MTT)的还原量来评估细胞活力。为了评估体外脂肪变性,用冷磷酸盐缓冲盐水(PBS)冲洗细胞,并在4%多聚甲醛中固定。使用光学显微镜捕获细胞图像,然后用异丙醇(60%)提取染色的脂滴,通过测量其在510 nm处的吸光度进行定量。结果:mtt试验表明,PPF在24 h和48 h的所有浓度下都具有细胞毒性。浓度为1-10 ppm时,细胞活力低于50%,浓度为100 ppm时,细胞活力低于10%。PPF诱导细胞内脂质从1ppm浓度开始增加。在100ppm时观察到最大的影响。讨论:在我们的体外研究中,通过测量线粒体酶活性的mtt测试,我们发现浓度为1- 10ppm时,细胞活力损失约50%。由于线粒体酶活性影响凋亡开始时的主要变化,这表明PPF对人肝细胞具有很强的细胞毒性。在HepG2的体外培养中,1ppm浓度的PPF已经诱导细胞内脂质的增加。
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引用次数: 4
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Occupational medicine and health affairs
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