Pub Date : 2020-01-01DOI: 10.23880/OAJVSR-16000194
Hassan Ra
The current experiment was conducted to assess the effect of using different litter types with or without alum on performance and litter quality of the hen layers. A total number of 120 Inshas layer hens of 24 weeks of age were randomly distributed into 4 groups (3 replicates each 10 hens) of floor litter: T1 birds were raised on wood shaving litter, T2 birds were raised on wheat straw litter, T3 birds were raised wood shaving with 100 g of alum / kg of bedding, and T4 birds were raised on wheat straw litter with 100 g of alum / kg of bedding. Topping of fresh litter with acidifier amendment improved body weight, egg weight, egg number, egg mass, feed intake and feed conversion ratio of layers compared to chickens in the untreated litter group. In comparison with the control group, alum treatment reduced the pH level of the litter. Regarding the NH4+-N content, alum-treated litter showed a higher value than the untreated litter. On average, alum-treated litter had lower P content than the untreated litter. Alum treated group showed a significant increase in dry matter and total nitrogen content when compared with the untreated group. The study results showed significant variability (p < .05) in the moisture content and pH of the control and treated litter. This clearly shows the effectiveness of litter treatment products enhancing litter quality and therefore the bird’s environmental climate. Thus it can be decided that litter modification with alum treatment had a significant impact on litter quality and in turn enhanced the productive performance of layer chickens without any adverse effect.
{"title":"Effect of Alum (Aluminum Sulfate) Addition to Poultry Litter on Layer Performance, Litter pH and Litter Composition","authors":"Hassan Ra","doi":"10.23880/OAJVSR-16000194","DOIUrl":"https://doi.org/10.23880/OAJVSR-16000194","url":null,"abstract":"The current experiment was conducted to assess the effect of using different litter types with or without alum on performance and litter quality of the hen layers. A total number of 120 Inshas layer hens of 24 weeks of age were randomly distributed into 4 groups (3 replicates each 10 hens) of floor litter: T1 birds were raised on wood shaving litter, T2 birds were raised on wheat straw litter, T3 birds were raised wood shaving with 100 g of alum / kg of bedding, and T4 birds were raised on wheat straw litter with 100 g of alum / kg of bedding. Topping of fresh litter with acidifier amendment improved body weight, egg weight, egg number, egg mass, feed intake and feed conversion ratio of layers compared to chickens in the untreated litter group. In comparison with the control group, alum treatment reduced the pH level of the litter. Regarding the NH4+-N content, alum-treated litter showed a higher value than the untreated litter. On average, alum-treated litter had lower P content than the untreated litter. Alum treated group showed a significant increase in dry matter and total nitrogen content when compared with the untreated group. The study results showed significant variability (p < .05) in the moisture content and pH of the control and treated litter. This clearly shows the effectiveness of litter treatment products enhancing litter quality and therefore the bird’s environmental climate. Thus it can be decided that litter modification with alum treatment had a significant impact on litter quality and in turn enhanced the productive performance of layer chickens without any adverse effect.","PeriodicalId":19494,"journal":{"name":"Open Access Journal","volume":"6 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90825869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.23880/OAJVSR-16000204
Z. Hernández-Russo
Environmental conditions in Uruguay favour the development of gastrointestinal nematodes (GIN) throughout the year, with clinical or subclinical manifestations that cause significant economic losses. The aim of this study was to evaluate the effect of the GIN on fertility, fecundity, reproductive rate and lamb growth in Australian Merino sheep grazing on basalt fields. The study was carried out in a farm located in north of Uruguay. Sixty-eight Merino ewes facing a natural parasite challenge were divided into two groups. Control group was doused with active anthelmintic that have proven efficacy to minimize the effects of parasitic and the parasitized group that did not receive anthelmintic, except rescue dosages. Stool sample of each sheep was collected monthly for egg counts (EPG) of GIN using McMaster technique and nematode genders were identified from infective larvae obtained in cultures by Roberts O’ Sullivan technique. Artificial insemination and ewes were mated for new service, and pregnancy condition was diagnosed by ultrasound. Lambing control was made and lambs were weighed at birth and marking. The evolution of EPG values showed significant differences between groups at the end of gestation and lactation. Haemonchus spp. was the main gender of nematodes found, followed by Trichostrongylus spp. Fertility was 95 % versus 90 %; fecundity 87 % versus 63 % and reproductive rate 73 % versus 47 % for the control group and the parasitized one respectively. No significant differences were recorded in birth-weight. However market-weight and lamb daily gain were lower in the parasitized group. The effect of natural infection GIN was evident in lower reproductive performance of Australian Merino sheep.
乌拉圭的环境条件全年有利于胃肠道线虫(GIN)的发展，具有临床或亚临床表现，造成重大经济损失。摘要本试验旨在评价在玄武岩田中放牧的澳大利亚美利奴羊在不同条件下的育性、繁殖力、繁殖率和羔羊生长发育的影响。这项研究在乌拉圭北部的一个农场进行。68只面临天然寄生虫挑战的美利奴母羊被分成两组。对照组注射有效的驱虫药以减少寄生虫的影响，而被寄生组除抢救剂量外不注射驱虫药。采用麦克马斯特技术每月采集每只羊的粪便样本进行GIN虫卵计数(EPG)，并用Roberts O ' Sullivan技术从培养的感染幼虫中鉴定线虫性别。采用人工授精和母羊配种，超声诊断妊娠情况。进行羔羊对照，在羔羊出生和标记时称重。妊娠末期和哺乳期EPG值在各组间的演化有显著差异。发现的线虫性别以血螨为主，其次为毛线虫，育性分别为95%和90%;对照组和被寄生蝇的繁殖力分别为87%和63%，繁殖率分别为73%和47%。出生体重无显著差异。而寄生组羔羊的市场体重和日增重较低。自然感染GIN对澳大利亚美利奴羊繁殖性能降低的影响明显。
{"title":"Effect of Gastrointestinal Nematodes on Reproduction and Lamb Growth in Australian Merino Sheep","authors":"Z. Hernández-Russo","doi":"10.23880/OAJVSR-16000204","DOIUrl":"https://doi.org/10.23880/OAJVSR-16000204","url":null,"abstract":"Environmental conditions in Uruguay favour the development of gastrointestinal nematodes (GIN) throughout the year, with clinical or subclinical manifestations that cause significant economic losses. The aim of this study was to evaluate the effect of the GIN on fertility, fecundity, reproductive rate and lamb growth in Australian Merino sheep grazing on basalt fields. The study was carried out in a farm located in north of Uruguay. Sixty-eight Merino ewes facing a natural parasite challenge were divided into two groups. Control group was doused with active anthelmintic that have proven efficacy to minimize the effects of parasitic and the parasitized group that did not receive anthelmintic, except rescue dosages. Stool sample of each sheep was collected monthly for egg counts (EPG) of GIN using McMaster technique and nematode genders were identified from infective larvae obtained in cultures by Roberts O’ Sullivan technique. Artificial insemination and ewes were mated for new service, and pregnancy condition was diagnosed by ultrasound. Lambing control was made and lambs were weighed at birth and marking. The evolution of EPG values showed significant differences between groups at the end of gestation and lactation. Haemonchus spp. was the main gender of nematodes found, followed by Trichostrongylus spp. Fertility was 95 % versus 90 %; fecundity 87 % versus 63 % and reproductive rate 73 % versus 47 % for the control group and the parasitized one respectively. No significant differences were recorded in birth-weight. However market-weight and lamb daily gain were lower in the parasitized group. The effect of natural infection GIN was evident in lower reproductive performance of Australian Merino sheep.","PeriodicalId":19494,"journal":{"name":"Open Access Journal","volume":"1 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88541039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.23880/OAJVSR-16000200
A. M. Osman
Background: Tick-borne diseases (TBDs) cause significant losses among livestock and impact the livelihoods of livestock communities worldwide including Somalia. Infections with Babesia and Theileria species are endemic in Somalia yet there is a lack of adequate information on their prevalence. Objective: The present study aimed to assess the prevalence of Cattle Piroplasmosis in three districts of Lower Shabelle Region, Somalia using microscope and to identify the available tick species found in the sampled cattle during the study. Methods: A total of 127 Cattle blood were examined for Piroplasms (Babesia and Theileria) using Microscope during January to February 2020. The available ticks were collected in 70% Ethanol. Results: The overall prevalence of Piroplasmosis was 123/127 (96.9% 95% CI: 92.1-99.1%). About 45 out of 127 (35.4% 95% CI: 27.1-44.1%) samples were positive for either Theileria or Babesia, while 78 out 127 (61.4% 95% CI: 52.4-69.9%) samples were mixed infected with both them. Risk factors like age, body condition, sex, lymphnode enlargement and presence of ticks were found to be insignificantly associated with Babesiosis and Theileriosis. For both infections the mean PCV of infected animals was insignificantly lower than non-infected animals. Six tick species belonging to two genera (Rhipicephalus and Amblyomma) were identified in the investigated cattle, including, R. pulchellus (64.7%), R. (Boophilus) microplus, (2%) R. (Boophilus) decoloratus (2%), R.evertsi, (3.9%) A. gemma (15.7%) and A. lepidum (11.8%). Conclusion: This study revealed a very high prevalence of Piroplasmosis in the study area and co-infections were more common than single infections. Further studies in other areas in the country are recommended.
{"title":"Prevalence of Cattle Piroplasmosis and Some Associated Risk Factors in Lower Shabelle Region, Somalia","authors":"A. M. Osman","doi":"10.23880/OAJVSR-16000200","DOIUrl":"https://doi.org/10.23880/OAJVSR-16000200","url":null,"abstract":"Background: Tick-borne diseases (TBDs) cause significant losses among livestock and impact the livelihoods of livestock communities worldwide including Somalia. Infections with Babesia and Theileria species are endemic in Somalia yet there is a lack of adequate information on their prevalence. Objective: The present study aimed to assess the prevalence of Cattle Piroplasmosis in three districts of Lower Shabelle Region, Somalia using microscope and to identify the available tick species found in the sampled cattle during the study. Methods: A total of 127 Cattle blood were examined for Piroplasms (Babesia and Theileria) using Microscope during January to February 2020. The available ticks were collected in 70% Ethanol. Results: The overall prevalence of Piroplasmosis was 123/127 (96.9% 95% CI: 92.1-99.1%). About 45 out of 127 (35.4% 95% CI: 27.1-44.1%) samples were positive for either Theileria or Babesia, while 78 out 127 (61.4% 95% CI: 52.4-69.9%) samples were mixed infected with both them. Risk factors like age, body condition, sex, lymphnode enlargement and presence of ticks were found to be insignificantly associated with Babesiosis and Theileriosis. For both infections the mean PCV of infected animals was insignificantly lower than non-infected animals. Six tick species belonging to two genera (Rhipicephalus and Amblyomma) were identified in the investigated cattle, including, R. pulchellus (64.7%), R. (Boophilus) microplus, (2%) R. (Boophilus) decoloratus (2%), R.evertsi, (3.9%) A. gemma (15.7%) and A. lepidum (11.8%). Conclusion: This study revealed a very high prevalence of Piroplasmosis in the study area and co-infections were more common than single infections. Further studies in other areas in the country are recommended.","PeriodicalId":19494,"journal":{"name":"Open Access Journal","volume":"76 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81205674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Reichardt, Christoph Wörz, M. Singer, M. Liewald, M. Henn, Daniel J. Förster, E. Zahedi, S. Boley, A. Feuer, V. Onuseit, G. Umlauf, Paul Reichle, J. Barz, G. Tovar, T. Hirth
A novel tribological system has been developed, in which volatile lubricants (carbon dioxide - CO$_{2}$ or nitrogen- N$_{2}$) are used as a substitute for mineral oil-based lubricants in deep drawing processes. This process enables the introduction of an intermediate medium under high pressure through flow-optimized, laser-drilled micro holes into the contact surfaces. This eliminates the need for subsequent, cost-intensive cleaning processes, as the volatile lubricants evaporate without leaving any residue during expansion to ambient pressure. The design of initial micro hole geometries was based on simulations of the flow behaviour of the lubricants passing through, which in turn were validated using pressure reactor tests. In addition, the wetting behaviour of CO$_{2}$ on relevant surfaces (tool surface and sheet material surface) was investigated experimentally using the captive-bubble-method. Thus, the optimal design of the micro holes (diameter, hole geometry and number of micro holes) could be determined using flat strip drawing tests. The optimal micro hole geometry determined in this way is suited for the use of both CO$_{2}$ and N$_{2}$ as volatile lubricant. Furthermore, extensive investigations for the production of the required micro hole geometry by laser drilling were carried out. The fundamentals for drilling micro holes in steel with high aspect ratios could be developed using an ultrashort pulsed research laser with very high pulse energy. Further experiments were conducted using an ultrashort pulsed prototype laser of the kW-class specially developed to increase productivity when drilling a multitude of micro holes with higher average laser power. The novel tribological system has been characterised by means of strip drawing tests and stretch bending tests. For both, CO$_{2}$ (liquid) and N$_{2}$ (gaseous), relatively low friction values could be achieved compared to conventional lubricants. It could be shown that deep drawing with both CO$_{2}$ and N$_{2}$ as dry lubricants is possible. Here, usage of the volatile lubricants not only allows the replacement of mineral oil based lubricants, but even improves the tribological system with regard to frictional forces in sheet metal forming. The feasibility of the new tribological system has been proven by performing deep drawing tests of rectangular cups. These tests showed a significantly enlarged process window of the forming process, which emphasise the tremendous potential of this new tribological system.
{"title":"Tribological system for cold sheet metal forming based on volatile lubricants and laser structured surfaces","authors":"G. Reichardt, Christoph Wörz, M. Singer, M. Liewald, M. Henn, Daniel J. Förster, E. Zahedi, S. Boley, A. Feuer, V. Onuseit, G. Umlauf, Paul Reichle, J. Barz, G. Tovar, T. Hirth","doi":"10.26092/ELIB/156","DOIUrl":"https://doi.org/10.26092/ELIB/156","url":null,"abstract":"A novel tribological system has been developed, in which volatile lubricants (carbon dioxide - CO$_{2}$ or nitrogen- N$_{2}$) are used as a substitute for mineral oil-based lubricants in deep drawing processes. This process enables the introduction of an intermediate medium under high pressure through flow-optimized, laser-drilled micro holes into the contact surfaces. This eliminates the need for subsequent, cost-intensive cleaning processes, as the volatile lubricants evaporate without leaving any residue during expansion to ambient pressure. The design of initial micro hole geometries was based on simulations of the flow behaviour of the lubricants passing through, which in turn were validated using pressure reactor tests. In addition, the wetting behaviour of CO$_{2}$ on relevant surfaces (tool surface and sheet material surface) was investigated experimentally using the captive-bubble-method. Thus, the optimal design of the micro holes (diameter, hole geometry and number of micro holes) could be determined using flat strip drawing tests. The optimal micro hole geometry determined in this way is suited for the use of both CO$_{2}$ and N$_{2}$ as volatile lubricant. Furthermore, extensive investigations for the production of the required micro hole geometry by laser drilling were carried out. The fundamentals for drilling micro holes in steel with high aspect ratios could be developed using an ultrashort pulsed research laser with very high pulse energy. Further experiments were conducted using an ultrashort pulsed prototype laser of the kW-class specially developed to increase productivity when drilling a multitude of micro holes with higher average laser power. The novel tribological system has been characterised by means of strip drawing tests and stretch bending tests. For both, CO$_{2}$ (liquid) and N$_{2}$ (gaseous), relatively low friction values could be achieved compared to conventional lubricants. It could be shown that deep drawing with both CO$_{2}$ and N$_{2}$ as dry lubricants is possible. Here, usage of the volatile lubricants not only allows the replacement of mineral oil based lubricants, but even improves the tribological system with regard to frictional forces in sheet metal forming. The feasibility of the new tribological system has been proven by performing deep drawing tests of rectangular cups. These tests showed a significantly enlarged process window of the forming process, which emphasise the tremendous potential of this new tribological system.","PeriodicalId":19494,"journal":{"name":"Open Access Journal","volume":"92 1","pages":"128"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75442806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.23880/OAJVSR-16000196
Ciornei Sg
It is emphasized that the medical staff is subject to a maximum risk, and so it is. Due to permanent contact with potentially contaminated patients, carriers may be infected or carry the virus. In this context, it is highlighted that veterinarians may have a high risk of infection. In the world literature, and international epizootic forums, it reports and publishes information on the presence of COVID19 in animals. Some animals have been confirmed with this virus, generally pets that come from families where there have been several carrier members. Pets that tested positive showed clinical signs of dyspnea, fever, impaired general condition, difficulty breathing. The International Office of Epizootics cites a few scattered cases in which they tested positive in laboratory tests, but their study led to some findings essential for understanding the epidemiology of the new disease. Obviously, in the case of dogs and cats found positive, the SARS-CoV-2 infection was linked to the situation of the owners, who were also positive. The thorough investigations revealed that in fact dogs and cats had not been the source of infection of the owners, but, they contracted the infection from their owners. And more clearly, it was not humans who turned out to be victims of animals, but vice versa. Among pets, cats and ferrets are the most blamed, they were also present in clinical manifestations and may possibly transmit the disease to other cats. Dogs don’t seem to be as sensitive. Veterinary medicine is in this situation caught in the middle: on the one hand it is obliged to investigate the possible source of animal infection, using its own and specific means of investigation, but on the other hand it has the professional duty to defend the innocence of some animal species, unjustly suspected and possibly incriminated. Let’s not forget that Covid 19 is a syndrome not a specific disease, and therefore clinical confusions are not impossible.
{"title":"SARS-CoV-2 in Animals-Evolution and Associated Risks","authors":"Ciornei Sg","doi":"10.23880/OAJVSR-16000196","DOIUrl":"https://doi.org/10.23880/OAJVSR-16000196","url":null,"abstract":"It is emphasized that the medical staff is subject to a maximum risk, and so it is. Due to permanent contact with potentially contaminated patients, carriers may be infected or carry the virus. In this context, it is highlighted that veterinarians may have a high risk of infection. In the world literature, and international epizootic forums, it reports and publishes information on the presence of COVID19 in animals. Some animals have been confirmed with this virus, generally pets that come from families where there have been several carrier members. Pets that tested positive showed clinical signs of dyspnea, fever, impaired general condition, difficulty breathing. The International Office of Epizootics cites a few scattered cases in which they tested positive in laboratory tests, but their study led to some findings essential for understanding the epidemiology of the new disease. Obviously, in the case of dogs and cats found positive, the SARS-CoV-2 infection was linked to the situation of the owners, who were also positive. The thorough investigations revealed that in fact dogs and cats had not been the source of infection of the owners, but, they contracted the infection from their owners. And more clearly, it was not humans who turned out to be victims of animals, but vice versa. Among pets, cats and ferrets are the most blamed, they were also present in clinical manifestations and may possibly transmit the disease to other cats. Dogs don’t seem to be as sensitive. Veterinary medicine is in this situation caught in the middle: on the one hand it is obliged to investigate the possible source of animal infection, using its own and specific means of investigation, but on the other hand it has the professional duty to defend the innocence of some animal species, unjustly suspected and possibly incriminated. Let’s not forget that Covid 19 is a syndrome not a specific disease, and therefore clinical confusions are not impossible.","PeriodicalId":19494,"journal":{"name":"Open Access Journal","volume":"31 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78882515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.23880/OAJVSR-16000197
Á. Mesterházy
All mistakes in plant breeding, plant production, storage will be manifested in the animal husbandry and will become a veterinary problem with far reaching economic consequences. The managing of the field originated toxins is far not enough because the cleaning losses are high, toxin binding is only moderately effective and expensive and this is valid for the use of antibiotics. We should prevent toxin contamination in the field as far it is possible with varieties and hybrids with higher and increasing resistance, better agronomy and plant protection. A well-organized harvest (separation of the grain according toxin contamination), excellent storage facilities and management are also highly important. The veterinary side can be successful when cooperates with the breeding and production and delivers the results supporting better limits for toxins and support extensive prevention also in the government.
{"title":"How to Avoid Toxigenic Problems in Animal Husbandry?","authors":"Á. Mesterházy","doi":"10.23880/OAJVSR-16000197","DOIUrl":"https://doi.org/10.23880/OAJVSR-16000197","url":null,"abstract":"All mistakes in plant breeding, plant production, storage will be manifested in the animal husbandry and will become a veterinary problem with far reaching economic consequences. The managing of the field originated toxins is far not enough because the cleaning losses are high, toxin binding is only moderately effective and expensive and this is valid for the use of antibiotics. We should prevent toxin contamination in the field as far it is possible with varieties and hybrids with higher and increasing resistance, better agronomy and plant protection. A well-organized harvest (separation of the grain according toxin contamination), excellent storage facilities and management are also highly important. The veterinary side can be successful when cooperates with the breeding and production and delivers the results supporting better limits for toxins and support extensive prevention also in the government.","PeriodicalId":19494,"journal":{"name":"Open Access Journal","volume":"93 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89744102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.23880/OAJVSR-16000205
M. Filimonova
The nuclease from S.marcescens heads a family of homological nonspecific nucleases. Its cultivation features, structure, mechanism of action, some physical, chemical and biochemical properties are well studied. It is presented by two isoforms, differing by N-terminal fragment and some properties. The nuclease demonstrated a cancer suppressing effect in mice. It is a key component of the anti-rabies composition which increases survival of the infected mice. It is similar by its efficiency to Pulmozyme® that is used for airway cleansing during the treatment of pulmonary diseases. The aim of the study was to analyze a cytotoxic effect of S. marcescens nuclease and its separate isoforms at different concentrations. To determine the cytotoxicity we used MTT assay and human cell cultures of colorectal cancer -HCT 116, breast cancer MCF-7 and normal skin fibroblasts. Results show that the nuclease demonstrates a weak cytotoxic effect at the amounts of 0.2 – 25 µg per 1 ml of the cell culture medium that is close to its therapeutic doses. This effect is not connected with the loss of the enzymatic activity. The isoforms are similar by their IC50.
{"title":"The Nuclease from Gram-Negative Bacteria Serratia Marcescens is Weakly Cytotoxic at Therapeutic Doses","authors":"M. Filimonova","doi":"10.23880/OAJVSR-16000205","DOIUrl":"https://doi.org/10.23880/OAJVSR-16000205","url":null,"abstract":"The nuclease from S.marcescens heads a family of homological nonspecific nucleases. Its cultivation features, structure, mechanism of action, some physical, chemical and biochemical properties are well studied. It is presented by two isoforms, differing by N-terminal fragment and some properties. The nuclease demonstrated a cancer suppressing effect in mice. It is a key component of the anti-rabies composition which increases survival of the infected mice. It is similar by its efficiency to Pulmozyme® that is used for airway cleansing during the treatment of pulmonary diseases. The aim of the study was to analyze a cytotoxic effect of S. marcescens nuclease and its separate isoforms at different concentrations. To determine the cytotoxicity we used MTT assay and human cell cultures of colorectal cancer -HCT 116, breast cancer MCF-7 and normal skin fibroblasts. Results show that the nuclease demonstrates a weak cytotoxic effect at the amounts of 0.2 – 25 µg per 1 ml of the cell culture medium that is close to its therapeutic doses. This effect is not connected with the loss of the enzymatic activity. The isoforms are similar by their IC50.","PeriodicalId":19494,"journal":{"name":"Open Access Journal","volume":"23 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90804439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.23880/OAJVSR-16000198
H. H. Nazht
The present study designed to focus on the advantages and disadvantages of using food grate stainless steel rods (FGSR) as internal fixation methods for induced transverse fractures in the mid shift of the femoral bones in rabbits.200 cases were collected from 2007 to 2020, all these cases were employed to induced transverse fractures in the mid shift of femoral bone, 100 of them used the rods for internal fixation, 80 of the cases used the rods for fixation the natural xenbony implantation from sheep or calves, and the others 20 cases used the rods for internal fixation of the synthetics nano bony implantation .The physical, chemical, clinical and radiographic parameters were used for evaluation, the physical and chemical analysis showed that the rods not change during sterilization, implantation or when exposed to different types of ray besides the chemical constant is about the same level measurement of the medical intramedullary pins (IMP), while the clinical observation revealed that the rods can used strongly and successfully for fracture fixation and support the animal to bear the weight, the limb used for walking gradually after 24-48 hours p. o. with some cases shown the FGSR pulled from the bone, other suffer from infection with pus formation with lateral deviation of the stifle joint or re-fracture. While the radiological finding revealed that the FGSR insert and fix the fractures fragment and the bony implantation, the pins seemed stable and fit the intramedullary canal and fracture fragments, while some cases shows that the pin not insert properly inside the femoral bone with case of multible and comminuted fractures, other case shown the FGSR pass and penetrate the stifle joint. The conclusion is, there are many advantages with minor or very little disadvantages for using the FGSR as internal fixation of the induced transverse fractures in the mid shift of the femoral bones and for natural and synthetics bony implantation in rabbits.
{"title":"Advantages and Disadvantages of using Food Grate Stainless Steel Rods for Internal Fixation of Femoral Transverse Fractures in Rabbits (Review Study)","authors":"H. H. Nazht","doi":"10.23880/OAJVSR-16000198","DOIUrl":"https://doi.org/10.23880/OAJVSR-16000198","url":null,"abstract":"The present study designed to focus on the advantages and disadvantages of using food grate stainless steel rods (FGSR) as internal fixation methods for induced transverse fractures in the mid shift of the femoral bones in rabbits.200 cases were collected from 2007 to 2020, all these cases were employed to induced transverse fractures in the mid shift of femoral bone, 100 of them used the rods for internal fixation, 80 of the cases used the rods for fixation the natural xenbony implantation from sheep or calves, and the others 20 cases used the rods for internal fixation of the synthetics nano bony implantation .The physical, chemical, clinical and radiographic parameters were used for evaluation, the physical and chemical analysis showed that the rods not change during sterilization, implantation or when exposed to different types of ray besides the chemical constant is about the same level measurement of the medical intramedullary pins (IMP), while the clinical observation revealed that the rods can used strongly and successfully for fracture fixation and support the animal to bear the weight, the limb used for walking gradually after 24-48 hours p. o. with some cases shown the FGSR pulled from the bone, other suffer from infection with pus formation with lateral deviation of the stifle joint or re-fracture. While the radiological finding revealed that the FGSR insert and fix the fractures fragment and the bony implantation, the pins seemed stable and fit the intramedullary canal and fracture fragments, while some cases shows that the pin not insert properly inside the femoral bone with case of multible and comminuted fractures, other case shown the FGSR pass and penetrate the stifle joint. The conclusion is, there are many advantages with minor or very little disadvantages for using the FGSR as internal fixation of the induced transverse fractures in the mid shift of the femoral bones and for natural and synthetics bony implantation in rabbits.","PeriodicalId":19494,"journal":{"name":"Open Access Journal","volume":"32 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88083808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.23880/OAJVSR-16000191
Getnet Zemenu Adiss
Porcine circovirus is an important viral species in the genus circovirus. It causes an immerse economic losses in the piggery industry. According to the retrospective studies, PCV2 has circulated before its acclaimed detection from samples taken as of the first outbreak in Canada. A bit far on in time, it has been reported in Europe, United States and Asian countries. The disease is endemic in most pig producing countries. Since then phylogeny studies supported for the immergence of various new Porcine circoviruses variants and genotypes. In addition to its natural reservoirs (wild and feral pigs), it also inhibits calves, goats, canines and mice. Some insects like mosquitoes are also the potential carrier of PCV2 even let it for cross species transmission. Hence those proper prevention measures of the mechanical carrier vectors of the disease should be noted together with the need of efficient vaccine against the pathogenic porcine circoviruses types.
{"title":"Porcine Circovirus: Historical Outlooks and Non-Porcine Victims","authors":"Getnet Zemenu Adiss","doi":"10.23880/OAJVSR-16000191","DOIUrl":"https://doi.org/10.23880/OAJVSR-16000191","url":null,"abstract":"Porcine circovirus is an important viral species in the genus circovirus. It causes an immerse economic losses in the piggery industry. According to the retrospective studies, PCV2 has circulated before its acclaimed detection from samples taken as of the first outbreak in Canada. A bit far on in time, it has been reported in Europe, United States and Asian countries. The disease is endemic in most pig producing countries. Since then phylogeny studies supported for the immergence of various new Porcine circoviruses variants and genotypes. In addition to its natural reservoirs (wild and feral pigs), it also inhibits calves, goats, canines and mice. Some insects like mosquitoes are also the potential carrier of PCV2 even let it for cross species transmission. Hence those proper prevention measures of the mechanical carrier vectors of the disease should be noted together with the need of efficient vaccine against the pathogenic porcine circoviruses types.","PeriodicalId":19494,"journal":{"name":"Open Access Journal","volume":"39 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79178699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.23880/OAJVSR-16000203
O. M. Abdi
Background: Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. Capripneumoniae (Mccp), is one of the most important goat diseases causing high morbidity and mortality, these results in heavy economic losses to countries like Somalia, which is a country affected the civil war of the 1990s. Objective: Generally, the disease presence has been reported in the country. However, there is no quantitative data on the prevalence and distribution of the disease has been scientifically reported in the country. Methods: A cross-sectional study was conducted from September 2019 to June 2020 in three villages of Afgoye district of Somalia to assess the seroprevalence and identify the associated risk factors for the occurrence of the CCPP. The risk factors considered included sex, age, and locations. Results: A total of 100 blood samples were collected and evaluated by Capri-Latex Agglutination Test (Capri-LAT). A total of 49/100 (49%, CI 95%: (38.86%-59.2%) goats were seropositive for anti-CCPP antibody. The epidemiological risk factors considered, sex, age and locations, were not significantly associated with the CCPP sero prevalence (p>0.05). However, the sero positivity was slightly higher in female (51%, 95% CI: 39.8-62.6), >3 years old goats (86.3%, 95% CI: 48.9-87.4) and Markazka village (56%, 95% CI: 41.3-70.0) compared to male (40%, 95% CI: 19.1-63.9), 1-3 years old (57.5%, 95% CI: 40.9- 73.0), and Shareeco (43.8%, 95% CI: 19.8-70.1) and Siinka (41.2%, 95% CI: 24.7-59.3) villages, respectively. Conclusion: The present study indicates the prevalence of CCPP in the studies areas and this requires practicable measures to be put in place towards the control of the disease.
{"title":"Sero-Prevalence of Contagious Caprine Pleuropneumonia in Goats in Afgoye District Lower Shabelle Region, Somalia","authors":"O. M. Abdi","doi":"10.23880/OAJVSR-16000203","DOIUrl":"https://doi.org/10.23880/OAJVSR-16000203","url":null,"abstract":"Background: Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. Capripneumoniae (Mccp), is one of the most important goat diseases causing high morbidity and mortality, these results in heavy economic losses to countries like Somalia, which is a country affected the civil war of the 1990s. Objective: Generally, the disease presence has been reported in the country. However, there is no quantitative data on the prevalence and distribution of the disease has been scientifically reported in the country. Methods: A cross-sectional study was conducted from September 2019 to June 2020 in three villages of Afgoye district of Somalia to assess the seroprevalence and identify the associated risk factors for the occurrence of the CCPP. The risk factors considered included sex, age, and locations. Results: A total of 100 blood samples were collected and evaluated by Capri-Latex Agglutination Test (Capri-LAT). A total of 49/100 (49%, CI 95%: (38.86%-59.2%) goats were seropositive for anti-CCPP antibody. The epidemiological risk factors considered, sex, age and locations, were not significantly associated with the CCPP sero prevalence (p>0.05). However, the sero positivity was slightly higher in female (51%, 95% CI: 39.8-62.6), >3 years old goats (86.3%, 95% CI: 48.9-87.4) and Markazka village (56%, 95% CI: 41.3-70.0) compared to male (40%, 95% CI: 19.1-63.9), 1-3 years old (57.5%, 95% CI: 40.9- 73.0), and Shareeco (43.8%, 95% CI: 19.8-70.1) and Siinka (41.2%, 95% CI: 24.7-59.3) villages, respectively. Conclusion: The present study indicates the prevalence of CCPP in the studies areas and this requires practicable measures to be put in place towards the control of the disease.","PeriodicalId":19494,"journal":{"name":"Open Access Journal","volume":"72 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90287408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：