Fang-Zhou Xu, Fu-Rong Meng, Wan-Jing Li, Lu Xu, Hao Zhang, Yan-Bei Zhang, Xiao-Yun Fan
The aim of this study is to assess the impact of serum magnesium (Mg) levels on prognostic outcomes in patients with non-small cell lung cancer (NSCLC) undergoing treatment with epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKI). A cohort comprising 91 patients with NSCLC with epidermal growth factor receptor mutations received EGFR-TKI therapy. Assessments of liver and kidney function and electrolyte levels were conducted before treatment initiation and after completing two cycles of EGFR-TKI therapy. Data on variables such as age, gender, presence of distant metastasis, smoking history, other therapeutic interventions, and the specific TKI used were collected for analysis. Cox regression analysis revealed that patients with higher Mg levels prior to EGFR-TKI therapy had significantly longer progression-free survival (PFS) and overall survival (OS). Elevated Mg levels remained predictive of PFS and OS after two cycles of EGFR-TKI therapy. Multiple regression analysis confirmed these findings. Additionally, it was observed that smokers might represent a unique population, demonstrating a correlation between OS and Mg levels. Our findings indicate that serum Mg level is a prognostic factor in patients with NSCLC undergoing EGFR-TKI therapy. This may provide new insights into the underlying mechanisms of EGFR-TKI therapy related to electrolyte balance.
{"title":"Predictive value of serum magnesium levels for prognosis in patients with non-small cell lung cancer undergoing EGFR-TKI therapy","authors":"Fang-Zhou Xu, Fu-Rong Meng, Wan-Jing Li, Lu Xu, Hao Zhang, Yan-Bei Zhang, Xiao-Yun Fan","doi":"10.1515/biol-2022-0923","DOIUrl":"https://doi.org/10.1515/biol-2022-0923","url":null,"abstract":"The aim of this study is to assess the impact of serum magnesium (Mg) levels on prognostic outcomes in patients with non-small cell lung cancer (NSCLC) undergoing treatment with epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKI). A cohort comprising 91 patients with NSCLC with epidermal growth factor receptor mutations received EGFR-TKI therapy. Assessments of liver and kidney function and electrolyte levels were conducted before treatment initiation and after completing two cycles of EGFR-TKI therapy. Data on variables such as age, gender, presence of distant metastasis, smoking history, other therapeutic interventions, and the specific TKI used were collected for analysis. Cox regression analysis revealed that patients with higher Mg levels prior to EGFR-TKI therapy had significantly longer progression-free survival (PFS) and overall survival (OS). Elevated Mg levels remained predictive of PFS and OS after two cycles of EGFR-TKI therapy. Multiple regression analysis confirmed these findings. Additionally, it was observed that smokers might represent a unique population, demonstrating a correlation between OS and Mg levels. Our findings indicate that serum Mg level is a prognostic factor in patients with NSCLC undergoing EGFR-TKI therapy. This may provide new insights into the underlying mechanisms of EGFR-TKI therapy related to electrolyte balance.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141779736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ji Zhao, Feng Shen, Yue-Mei Hu, Kai Yin, Ying Chen, Yan-Jie Chen, Qun-Chao Hu, Li Liang
This study aimed to determine the prognostic value and microenvironmental crosstalk of exosome-related signatures in human epidermal growth factor receptor 2 positive breast cancer (HER2+ BC). Transcriptome sequencing and clinicopathological data were downloaded from the Cancer Genome Atlas. The 10X single cell sequencing dataset was downloaded from the National Center for Biotechnology Information Gene Expression Omnibus. Exosomes-Related Genes were extracted from the ExoCarta and Gene Set Enrichment Analysis databases. FGF9, SF3B4, and EPCAM were found and deemed the most accurate predictive signatures. Patients with HER2+ BC were subtyped into three groupings by exosome prognostic gene (EPGs). The expression of SF3B4 was positively linked with the infiltration of macrophages, neutrophils, and CD4+ T cells. The expression characteristics of EPGs were associated with the biological process of “response to xenobiotic stimuli.” Interactions were relatively high between malignant epithelial cells and fibroblasts, endothelial cells, monocytes, and macrophages. Malignant epithelial cells interact more with fibroblasts and endothelial cells. The migration inhibitory factor pathway was the primary outgoing signaling pattern, while the C-C motif chemokine ligand pathway was the primary incoming signaling pattern for communication between malignant epithelial cells and macrophages. This study described the role of exosome signatures in the prognosis and microenvironment of HER2+ BC and provided a basis for future research.
本研究旨在确定外泌体相关特征在人类表皮生长因子受体2阳性乳腺癌（HER2+ BC）中的预后价值和微环境串扰。转录组测序和临床病理数据均从癌症基因组图谱下载。10倍单细胞测序数据集从美国国家生物技术信息中心基因表达总库（National Center for Biotechnology Information Gene Expression Omnibus）下载。外泌体相关基因提取自 ExoCarta 和基因组富集分析数据库。发现了FGF9、SF3B4和EPCAM，并认为它们是最准确的预测特征。根据外泌体预后基因（EPGs）将HER2+ BC患者分为三组。SF3B4的表达与巨噬细胞、中性粒细胞和CD4+ T细胞的浸润呈正相关。EPGs的表达特征与 "对异生物刺激的反应 "这一生物学过程有关。恶性上皮细胞与成纤维细胞、内皮细胞、单核细胞和巨噬细胞之间的相互作用相对较高。恶性上皮细胞与成纤维细胞和内皮细胞的相互作用较多。迁移抑制因子通路是恶性上皮细胞与巨噬细胞之间主要的传出信号模式，而C-C motif趋化因子配体通路则是主要的传入信号模式。这项研究描述了外泌体特征在HER2+ BC的预后和微环境中的作用，为今后的研究提供了基础。
{"title":"Prognostic value and microenvironmental crosstalk of exosome-related signatures in human epidermal growth factor receptor 2 positive breast cancer","authors":"Ji Zhao, Feng Shen, Yue-Mei Hu, Kai Yin, Ying Chen, Yan-Jie Chen, Qun-Chao Hu, Li Liang","doi":"10.1515/biol-2022-0899","DOIUrl":"https://doi.org/10.1515/biol-2022-0899","url":null,"abstract":"This study aimed to determine the prognostic value and microenvironmental crosstalk of exosome-related signatures in human epidermal growth factor receptor 2 positive breast cancer (HER2<jats:sup>+</jats:sup> BC). Transcriptome sequencing and clinicopathological data were downloaded from the Cancer Genome Atlas. The 10X single cell sequencing dataset was downloaded from the National Center for Biotechnology Information Gene Expression Omnibus. Exosomes-Related Genes were extracted from the ExoCarta and Gene Set Enrichment Analysis databases. FGF9, SF3B4, and EPCAM were found and deemed the most accurate predictive signatures. Patients with HER2<jats:sup>+</jats:sup> BC were subtyped into three groupings by exosome prognostic gene (EPGs). The expression of SF3B4 was positively linked with the infiltration of macrophages, neutrophils, and CD4<jats:sup>+</jats:sup> T cells. The expression characteristics of EPGs were associated with the biological process of “response to xenobiotic stimuli.” Interactions were relatively high between malignant epithelial cells and fibroblasts, endothelial cells, monocytes, and macrophages. Malignant epithelial cells interact more with fibroblasts and endothelial cells. The migration inhibitory factor pathway was the primary outgoing signaling pattern, while the C-C motif chemokine ligand pathway was the primary incoming signaling pattern for communication between malignant epithelial cells and macrophages. This study described the role of exosome signatures in the prognosis and microenvironment of HER2<jats:sup>+</jats:sup> BC and provided a basis for future research.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141779743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ruomu Ge, Zhengyun Luan, Ting Guo, Sheng Xia, Jun Ye, Jie Xu
The present work focused on investigating the role of the altered expression of complement C1s in proliferation and apoptosis of esophageal squamous cell carcinoma (ESCC) cells and explore its biological functions in ESCC, so as to lay a theoretical foundation and provide certain clinical reference for diagnosing and treating ESCC. Complement C1s expression within ESCC was assessed, and its clinical pathological characteristics in ESCC patients were analyzed. Subsequently, in vitro experiments were performed to further explore the mechanisms by which complement C1s affected ESCC. According to the results, complement C1s expression within ESCC markedly increased relative to adjacent non-cancerous samples. High C1s expression showed positive relation to race, residual lesion, and tumor location of ESCC patients. Complement C1s affected ESCC cell proliferation and apoptosis. Notably, C1s knockdown significantly inhibited ESCC cell proliferation and enhanced their apoptosis. C1s suppressed ESCC cell proliferation via Wnt1/β-catenin pathway and promoted their apoptosis through modulating the expression of Bcl2, Bax, and cleaved-caspase3.
{"title":"The expression and biological role of complement C1s in esophageal squamous cell carcinoma","authors":"Ruomu Ge, Zhengyun Luan, Ting Guo, Sheng Xia, Jun Ye, Jie Xu","doi":"10.1515/biol-2022-0915","DOIUrl":"https://doi.org/10.1515/biol-2022-0915","url":null,"abstract":"The present work focused on investigating the role of the altered expression of complement C1s in proliferation and apoptosis of esophageal squamous cell carcinoma (ESCC) cells and explore its biological functions in ESCC, so as to lay a theoretical foundation and provide certain clinical reference for diagnosing and treating ESCC. Complement C1s expression within ESCC was assessed, and its clinical pathological characteristics in ESCC patients were analyzed. Subsequently, <jats:italic>in vitro</jats:italic> experiments were performed to further explore the mechanisms by which complement C1s affected ESCC. According to the results, complement C1s expression within ESCC markedly increased relative to adjacent non-cancerous samples. High C1s expression showed positive relation to race, residual lesion, and tumor location of ESCC patients. Complement C1s affected ESCC cell proliferation and apoptosis. Notably, C1s knockdown significantly inhibited ESCC cell proliferation and enhanced their apoptosis. C1s suppressed ESCC cell proliferation via Wnt1/β-catenin pathway and promoted their apoptosis through modulating the expression of Bcl2, Bax, and cleaved-caspase3.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141779740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pseudohypoparathyroidism (PHP) type 1a (PHP 1a) is a rare hereditary disorder characterized by target organ resistance to hormonal signaling and the Albright hereditary osteodystrophy (AHO) phenotype, which features round facial features, short fingers, subcutaneous calcifications, short stature, obesity, and intellectual disability. Progressive osseous heteroplasia (POH) is another rare disorder characterized by heterotopic ossification (HO) that progressively affects skin, subcutaneous tissues, and deep skeletal muscle. PHP 1a is inherited maternally due to a GNAS mutation, while pure POH is inherited paternally. This case study presented a Chinese boy with congenital hypothyroidism, tonic-clonic seizures, hypoparathyroidism, AHO, POH, and joint fixation deformity. Sequencing analysis of GNAS-Gsα revealed a heterozygous C.432+2T>C(P.?) variant (NM_000516.7) affecting the canonical splice donor site of intron 5 in the boy and his mother, indicating maternal inheritance of a GNAS mutation. The patient was diagnosed with POH overlap syndrome (POH/PHP 1a). Following calcium and calcitriol supplementation, he experienced a reduction in seizures, and surgery was performed to correct the joint fixation deformity caused by HO. This case report provided valuable insights into the genotype-phenotype correlations of POH overlap syndrome and underscored the significance of genetic testing in diagnosing rare diseases.
{"title":"A novel GNAS mutation in pseudohypoparathyroidism type 1a with articular flexion deformity: A case report","authors":"Jinxing Wan, Dongjuan He, Jun Xie, Zhizhi Chen","doi":"10.1515/biol-2022-0918","DOIUrl":"https://doi.org/10.1515/biol-2022-0918","url":null,"abstract":"Pseudohypoparathyroidism (PHP) type 1a (PHP 1a) is a rare hereditary disorder characterized by target organ resistance to hormonal signaling and the Albright hereditary osteodystrophy (AHO) phenotype, which features round facial features, short fingers, subcutaneous calcifications, short stature, obesity, and intellectual disability. Progressive osseous heteroplasia (POH) is another rare disorder characterized by heterotopic ossification (HO) that progressively affects skin, subcutaneous tissues, and deep skeletal muscle. PHP 1a is inherited maternally due to a GNAS mutation, while pure POH is inherited paternally. This case study presented a Chinese boy with congenital hypothyroidism, tonic-clonic seizures, hypoparathyroidism, AHO, POH, and joint fixation deformity. Sequencing analysis of GNAS-Gsα revealed a heterozygous C.432+2T>C(P.?) variant (NM_000516.7) affecting the canonical splice donor site of intron 5 in the boy and his mother, indicating maternal inheritance of a GNAS mutation. The patient was diagnosed with POH overlap syndrome (POH/PHP 1a). Following calcium and calcitriol supplementation, he experienced a reduction in seizures, and surgery was performed to correct the joint fixation deformity caused by HO. This case report provided valuable insights into the genotype-phenotype correlations of POH overlap syndrome and underscored the significance of genetic testing in diagnosing rare diseases.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141779741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rajapaksha Welhenage Piumi Madhushika Rajapaksha, Don Padmapriya Shantha Thilak Gunasekera Attanayaka, Kalaivani Vivehananthan, Dennis McNevin
To date, the association of potato tuber microbiota is poorly understood. In this study, the endophytic bacterial flora of seed potato tubers was identified and the diversity of healthy and unhealthy tubers was compared. Metagenomic DNA extracted from healthy and unhealthy samples of seed potato tubers was used for the analysis of microbial communities. Next generation sequencing of the ∼460 bp v3–v4 region of the 16S rRNA gene was carried out using the Illumina Miseq platform. The data were analysed using the Divisive Amplicon Denoising Algorithm 2 pipeline. Sequence analysis of the potato metagenome identified amplicon sequence variants (ASVs) assigned to 745 different taxa belonging to eight Phyla: Firmicutes (46.2%), Proteobacteria (36.9%), Bacteroidetes (1.8%), Actinobacteria (0.1%), Tenericutes (0.005%), Saccharibacteria (0.003%), Verrucomicrobiota (0.003%), and Acidobacteria (0.001%). In healthy seed potato tubers, 55–99% of ASVs belonged to Firmicutes, including Bacillus, Salinibacillus, Staphylococcus, Lysinibacillus, Paenibacillus, and Brevibacillus genera within the taxonomic order Bacillales. However, in the visually unhealthy tubers, only 0.5–3.9% of ASVs belonged to Firmicutes while 84.1–97% of ASVs belonged to Proteobacteria. This study highlights that diverse bacterial communities colonize potato tubers, which contributes to the understanding of plant–microbe interactions and underscores the significance of metagenomic approaches in agricultural research.
迄今为止，人们对马铃薯块茎微生物群的关联还知之甚少。本研究鉴定了马铃薯块茎种子的内生细菌群，并比较了健康和不健康块茎的多样性。从健康和不健康的马铃薯块茎样本中提取的元基因组 DNA 被用于分析微生物群落。使用 Illumina Miseq 平台对 16S rRNA 基因的 ∼460 bp v3-v4 区域进行了新一代测序。数据采用分裂扩增子去噪算法 2（Divisive Amplicon Denoising Algorithm 2）管道进行分析。马铃薯元基因组的序列分析确定了属于八个门的 745 个不同类群的扩增子序列变体（ASVs）：它们分别是：固着菌（46.2%）、蛋白菌（36.9%）、类杆菌（1.8%）、放线菌（0.1%）、担子菌（0.005%）、糖杆菌（0.003%）、蛭弧菌（0.003%）和酸杆菌（0.001%）。在健康的马铃薯块茎种子中，55-99% 的 ASV 属于固着菌，包括芽孢杆菌属、盐杆菌属、葡萄球菌属、赖氨芽孢杆菌属、芽孢杆菌属和芽孢杆菌属。然而，在视觉上不健康的块茎中，只有 0.5-3.9% 的 ASV 属于固着菌，而 84.1-97% 的 ASV 属于变形菌。这项研究表明，马铃薯块茎中存在多种细菌群落，这有助于了解植物与微生物之间的相互作用，并强调了元基因组学方法在农业研究中的重要意义。
{"title":"Metagenomic analysis of endophytic bacteria in seed potato (Solanum tuberosum)","authors":"Rajapaksha Welhenage Piumi Madhushika Rajapaksha, Don Padmapriya Shantha Thilak Gunasekera Attanayaka, Kalaivani Vivehananthan, Dennis McNevin","doi":"10.1515/biol-2022-0897","DOIUrl":"https://doi.org/10.1515/biol-2022-0897","url":null,"abstract":"To date, the association of potato tuber microbiota is poorly understood. In this study, the endophytic bacterial flora of seed potato tubers was identified and the diversity of healthy and unhealthy tubers was compared. Metagenomic DNA extracted from healthy and unhealthy samples of seed potato tubers was used for the analysis of microbial communities. Next generation sequencing of the ∼460 bp v3–v4 region of the 16S rRNA gene was carried out using the Illumina Miseq platform. The data were analysed using the Divisive Amplicon Denoising Algorithm 2 pipeline. Sequence analysis of the potato metagenome identified amplicon sequence variants (ASVs) assigned to 745 different taxa belonging to eight Phyla: Firmicutes (46.2%), Proteobacteria (36.9%), Bacteroidetes (1.8%), Actinobacteria (0.1%), Tenericutes (0.005%), Saccharibacteria (0.003%), Verrucomicrobiota (0.003%), and Acidobacteria (0.001%). In healthy seed potato tubers, 55–99% of ASVs belonged to Firmicutes, including <jats:italic>Bacillus, Salinibacillus, Staphylococcus, Lysinibacillus, Paenibacillus</jats:italic>, and <jats:italic>Brevibacillus</jats:italic> genera within the taxonomic order Bacillales. However, in the visually unhealthy tubers, only 0.5–3.9% of ASVs belonged to Firmicutes while 84.1–97% of ASVs belonged to Proteobacteria. This study highlights that diverse bacterial communities colonize potato tubers, which contributes to the understanding of plant–microbe interactions and underscores the significance of metagenomic approaches in agricultural research.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141779739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Circular RNAs (circRNAs) play important roles in many human diseases. However, their role in the development of severe sepsis, a condition that remains one of the main causes of death in intensive care units, has not yet been defined. In this study, we interrogated the molecular mechanisms of circRNAs in severe sepsis. We profiled the expression levels of 5,680 circRNAs in plasma extracted from blood samples of 9 severe sepsis cases or 9 controls (male, age 78 ± 7) using the Human circRNA Array. To enrich protein-coding genes hosting severe sepsis-related circRNAs, we conducted gene ontology and pathways analyses. Out of the identified 760 differentially expressed circRNAs, 404 were upregulated while 356 were downregulated (fold change [FC] ≥2 or ≤−2, and false discovery ratio <0.05). Circ-0008285 (located in exons of CDYL), showed significant upregulation in severe sepsis with an FC of 13.7, and Bonferroni-corrected P < 0.05/5. In silico analysis identified Circ-0008285 interacting microRNAs as well as protein-coding genes. We systematically investigated the differential expression pattern of circRNAs in severe sepsis. The circRNAs we identified might serve as potential biomarkers for diagnosis and prognosis of sepsis.
{"title":"Circular RNAs as potential biomarkers for male severe sepsis","authors":"Liang Jun, Zhonghua Wang, Shouhong Wang, Xiaolong Liao, Tiehe Qin, Weixin Guo","doi":"10.1515/biol-2022-0900","DOIUrl":"https://doi.org/10.1515/biol-2022-0900","url":null,"abstract":"Circular RNAs (circRNAs) play important roles in many human diseases. However, their role in the development of severe sepsis, a condition that remains one of the main causes of death in intensive care units, has not yet been defined. In this study, we interrogated the molecular mechanisms of circRNAs in severe sepsis. We profiled the expression levels of 5,680 circRNAs in plasma extracted from blood samples of 9 severe sepsis cases or 9 controls (male, age 78 ± 7) using the Human circRNA Array. To enrich protein-coding genes hosting severe sepsis-related circRNAs, we conducted gene ontology and pathways analyses. Out of the identified 760 differentially expressed circRNAs, 404 were upregulated while 356 were downregulated (fold change [FC] ≥2 or ≤−2, and false discovery ratio <0.05). Circ-0008285 (located in exons of <jats:italic>CDYL</jats:italic>), showed significant upregulation in severe sepsis with an FC of 13.7, and Bonferroni-corrected <jats:italic>P</jats:italic> < 0.05/5. In silico analysis identified Circ-0008285 interacting microRNAs as well as protein-coding genes. We systematically investigated the differential expression pattern of circRNAs in severe sepsis. The circRNAs we identified might serve as potential biomarkers for diagnosis and prognosis of sepsis.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141779699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oral squamous cell carcinoma (OSCC) is the most common malignancy among head and neck squamous cell carcinomas. Targeted therapy plays a crucial role in the treatment of OSCC. However, new and more targets are still needed to develop. Stanniocalcin-1 (STC-1) is a glycoprotein hormone that affects the progression of cancers. However, the potential role of STC-1 in OSCC progression remains to be explored. Here, we aimed to elucidate the role of STC-1 in OSCC. We revealed that STC-1 was highly expressed in OSCC tissues and is correlated with poor patient prognosis. Knockdown of STC-1 significantly suppressed the growth of OSCC cells and restrained glycolysis by reducing glucose consumption, ATP production, and lactate levels. Mechanistically, STC-1 ablation inhibited the PI3K/Akt pathway, reducing the phosphorylation levels of PI3K and Akt. In conclusion, STC-1 depletion restrained OSCC cell growth and glycolysis via PI3K/Akt pathway and has the potential to serve as a therapeutic target for OSCC.
{"title":"Knockdown of Stanniocalcin-1 inhibits growth and glycolysis in oral squamous cell carcinoma cells","authors":"Chanyuan Wang, Jianpei Hu, Lijian Wang","doi":"10.1515/biol-2022-0907","DOIUrl":"https://doi.org/10.1515/biol-2022-0907","url":null,"abstract":"Oral squamous cell carcinoma (OSCC) is the most common malignancy among head and neck squamous cell carcinomas. Targeted therapy plays a crucial role in the treatment of OSCC. However, new and more targets are still needed to develop. Stanniocalcin-1 (STC-1) is a glycoprotein hormone that affects the progression of cancers. However, the potential role of STC-1 in OSCC progression remains to be explored. Here, we aimed to elucidate the role of STC-1 in OSCC. We revealed that STC-1 was highly expressed in OSCC tissues and is correlated with poor patient prognosis. Knockdown of STC-1 significantly suppressed the growth of OSCC cells and restrained glycolysis by reducing glucose consumption, ATP production, and lactate levels. Mechanistically, STC-1 ablation inhibited the PI3K/Akt pathway, reducing the phosphorylation levels of PI3K and Akt. In conclusion, STC-1 depletion restrained OSCC cell growth and glycolysis via PI3K/Akt pathway and has the potential to serve as a therapeutic target for OSCC.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141779738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The mortality rate of acute-on-chronic liver failure (ACLF) remains significantly elevated; hence, this study aimed to investigate the impact of heat shock protein family B (small) member 1 (HSPB1) on ACLF in vivo and in vitro and the underlying mechanism. This study used the ACLF mouse model, and liver damage extent was studied employing Masson trichrome, hematoxylin and eosin (H&E), Sirius red staining, and serum biochemical indices. Similarly, hepatocyte injury in lipopolysaccharide (LPS)-induced L02 cells was evaluated using cell counting kit-8 assay, enzymatic activity, flow cytometry, and TUNEL assay, while the underlying mechanism was investigated using western blot. Results showed that the morphology of liver tissue in ACLF mice was changed and was characterized by cirrhosis, fibrosis, collagen fiber deposition, inflammatory cell infiltration, and elevated liver injury indices. Moreover, HSPB1 was upregulated in both ACLF patients and mice, where overexpressing HSPB1 was found to inhibit ACLF-induced liver damage. Similarly, the HSPB1 expression in LPS-treated L02 cell lines was also increased, where overexpressing HSPB1 was found to promote cell viability, inhibit liver injury-related enzyme activity, and suppress apoptosis. Mechanistic investigations revealed that HSPB1 was responsible for inhibiting p-P53 and Bax protein levels, where activated P53 counteracted HSPB1’s effects on cellular behaviors. In conclusion, HSPB1 attenuated ACLF-induced liver injury in vivo and inhibited LPS-induced hepatocyte damage in vitro, suggesting that HSPB1 may be a novel target for ACLF therapy.
{"title":"HSPB1 alleviates acute-on-chronic liver failure via the P53/Bax pathway","authors":"Zhixiang Zhang, Jinwei Guo, Jincan Zhu","doi":"10.1515/biol-2022-0919","DOIUrl":"https://doi.org/10.1515/biol-2022-0919","url":null,"abstract":"The mortality rate of acute-on-chronic liver failure (ACLF) remains significantly elevated; hence, this study aimed to investigate the impact of heat shock protein family B (small) member 1 (HSPB1) on ACLF <jats:italic>in vivo</jats:italic> and <jats:italic>in vitro</jats:italic> and the underlying mechanism. This study used the ACLF mouse model, and liver damage extent was studied employing Masson trichrome, hematoxylin and eosin (H&E), Sirius red staining, and serum biochemical indices. Similarly, hepatocyte injury in lipopolysaccharide (LPS)-induced L02 cells was evaluated using cell counting kit-8 assay, enzymatic activity, flow cytometry, and TUNEL assay, while the underlying mechanism was investigated using western blot. Results showed that the morphology of liver tissue in ACLF mice was changed and was characterized by cirrhosis, fibrosis, collagen fiber deposition, inflammatory cell infiltration, and elevated liver injury indices. Moreover, HSPB1 was upregulated in both ACLF patients and mice, where overexpressing HSPB1 was found to inhibit ACLF-induced liver damage. Similarly, the HSPB1 expression in LPS-treated L02 cell lines was also increased, where overexpressing HSPB1 was found to promote cell viability, inhibit liver injury-related enzyme activity, and suppress apoptosis. Mechanistic investigations revealed that HSPB1 was responsible for inhibiting p-P53 and Bax protein levels, where activated P53 counteracted HSPB1’s effects on cellular behaviors. In conclusion, HSPB1 attenuated ACLF-induced liver injury <jats:italic>in vivo</jats:italic> and inhibited LPS-induced hepatocyte damage <jats:italic>in vitro</jats:italic>, suggesting that HSPB1 may be a novel target for ACLF therapy.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141779742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Considering the pear in the arid region as the research object, single-factor testing and water–fertilizer coupling testing were conducted. The response of pear tree growth to water, nitrogen, and phosphorus was explored and provided a theoretical basis for efficient water and fertilizer management. Among them, the single-factor test set water, nitrogen, and phosphorus as the three factors, and five levels were set. Screening out W3, W4, N3, N4, P3, and P4 promoted plant nutrient uptake and fruit quality. Eight treatments were set up in the water and fertilizer coupling test: Treatment 1 (T1, W3N3P3), Treatment 2 (T2, W3N3P4), Treatment 3 (T3, W3N4P3), Treatment 4 (T4, W3N4P4), Treatment 5 (T5, W4N3P3), Treatment 6 (T6, W4N3P4), Treatment 7 (T7, W4N4P3), and Treatment 8 (T8, W4N4P4). The results showed that the leaf area index of the T1, T2, T3, and T4 treatments was significantly higher than that of the other treatments at maturity. The yield, single fruit weight, and primary fruit rate were the highest under T3 treatment. The gray correlation degree analysis of fruit quality showed that the T3 treatment had the highest degree of correlation and ranking of each fruit quality index, indicating that the T3 treatment had the highest fruit quality. The yield model showed that irrigation with 6510.06 m3 hm−2, nitrogen fertilizer with 337.5 kg N hm−2, and phosphate fertilizer with 262.5 kg P hm−2 had the best yield. A detailed investigation of pear tree growth and fruit quality showed that the T3 treatment had the best fruit growth and development performance, and the pear fruit quality was the best.
以干旱地区梨树为研究对象，进行了单因素试验和水肥耦合试验。探讨了梨树生长对水、氮、磷的响应，为高效水肥管理提供了理论依据。其中，单因素试验设定水、氮、磷为三因素，共设五个水平。筛选出 W3、W4、N3、N4、P3 和 P4，促进了植物对养分的吸收，提高了果实品质。水肥耦合试验共设 8 个处理：处理 1（T1，W3N3P3）、处理 2（T2，W3N3P4）、处理 3（T3，W3N4P3）、处理 4（T4，W3N4P4）、处理 5（T5，W4N3P3）、处理 6（T6，W4N3P4）、处理 7（T7，W4N4P3）和处理 8（T8，W4N4P4）。结果表明，T1、T2、T3 和 T4 处理的叶面积指数在成熟期明显高于其他处理。T3处理的产量、单果重和初果率最高。果实品质的灰色关联度分析表明，T3 处理的果实品质各指标的关联度和排名均最高，表明 T3 处理的果实品质最高。产量模型显示，灌溉6510.06 m3 hm-2、氮肥337.5 kg N hm-2、磷肥262.5 kg P hm-2产量最高。对梨树生长和果实品质的详细调查表明，T3 处理的果实生长发育表现最好，梨果品质最佳。
{"title":"Exploring the coupling mode of water and fertilizer for improving growth, fruit quality, and yield of the pear in the arid region","authors":"Tianle Li, Zhijian Gao, Xinlu Bai, Sihai Yu, Shijie An, Qiangqing Zheng, Zhihui Tang, Jinhu Zhi","doi":"10.1515/biol-2022-0911","DOIUrl":"https://doi.org/10.1515/biol-2022-0911","url":null,"abstract":"Considering the pear in the arid region as the research object, single-factor testing and water–fertilizer coupling testing were conducted. The response of pear tree growth to water, nitrogen, and phosphorus was explored and provided a theoretical basis for efficient water and fertilizer management. Among them, the single-factor test set water, nitrogen, and phosphorus as the three factors, and five levels were set. Screening out W3, W4, N3, N4, P3, and P4 promoted plant nutrient uptake and fruit quality. Eight treatments were set up in the water and fertilizer coupling test: Treatment 1 (T1, W3N3P3), Treatment 2 (T2, W3N3P4), Treatment 3 (T3, W3N4P3), Treatment 4 (T4, W3N4P4), Treatment 5 (T5, W4N3P3), Treatment 6 (T6, W4N3P4), Treatment 7 (T7, W4N4P3), and Treatment 8 (T8, W4N4P4). The results showed that the leaf area index of the T1, T2, T3, and T4 treatments was significantly higher than that of the other treatments at maturity. The yield, single fruit weight, and primary fruit rate were the highest under T3 treatment. The gray correlation degree analysis of fruit quality showed that the T3 treatment had the highest degree of correlation and ranking of each fruit quality index, indicating that the T3 treatment had the highest fruit quality. The yield model showed that irrigation with 6510.06 m<jats:sup>3</jats:sup> hm<jats:sup>−2</jats:sup>, nitrogen fertilizer with 337.5 kg N hm<jats:sup>−2</jats:sup>, and phosphate fertilizer with 262.5 kg P hm<jats:sup>−2</jats:sup> had the best yield. A detailed investigation of pear tree growth and fruit quality showed that the T3 treatment had the best fruit growth and development performance, and the pear fruit quality was the best.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141779700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-20eCollection Date: 2024-01-01DOI: 10.1515/biol-2022-0884
Bingjun Gao, Xin Cheng, Yarong Wu, Boyi Jiang
Delayed or failed bone healing is a significant clinical challenge worldwide. Bone marrow mesenchymal stem cells (BMSCs) offer a promising approach for improving fracture healing. Isomangiferin, a xanthone C-glucoside, is known for its pharmacological activities, but its role in fracture healing remains unclear. In this study, we investigated the effects of isomangiferin on BMSCs under oxidative stress conditions induced by hydrogen peroxide (H2O2). Our results showed that isomangiferin promotes osteogenic differentiation and migration of H2O2-treated BMSCs, reduces apoptosis and reactive oxygen species production, and activates the AMP-activated protein kinase/acetyl-CoA carboxylase (AMPK/ACC) pathway. These findings suggest that isomangiferin may be a potential therapeutic agent for enhancing bone healing by modulating BMSC function.
{"title":"Isomangiferin promotes the migration and osteogenic differentiation of rat bone marrow mesenchymal stem cells.","authors":"Bingjun Gao, Xin Cheng, Yarong Wu, Boyi Jiang","doi":"10.1515/biol-2022-0884","DOIUrl":"10.1515/biol-2022-0884","url":null,"abstract":"<p><p>Delayed or failed bone healing is a significant clinical challenge worldwide. Bone marrow mesenchymal stem cells (BMSCs) offer a promising approach for improving fracture healing. Isomangiferin, a xanthone C-glucoside, is known for its pharmacological activities, but its role in fracture healing remains unclear. In this study, we investigated the effects of isomangiferin on BMSCs under oxidative stress conditions induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Our results showed that isomangiferin promotes osteogenic differentiation and migration of H<sub>2</sub>O<sub>2</sub>-treated BMSCs, reduces apoptosis and reactive oxygen species production, and activates the AMP-activated protein kinase/acetyl-CoA carboxylase (AMPK/ACC) pathway. These findings suggest that isomangiferin may be a potential therapeutic agent for enhancing bone healing by modulating BMSC function.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11259999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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