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Comparative transcriptome analysis of the antenna and proboscis reveals feeding state-dependent chemosensory genes in Eupeodes corollae. 触角和长鼻的比较转录组分析揭示了花冠大戟虫中依赖于摄食状态的化感基因。
IF 5.8 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 Epub Date: 2024-01-10 DOI: 10.1098/rsob.230208
Ruipeng Chen, Dong Ai, Guirong Wang, Bing Wang

The physiological state of an insect can affect its olfactory system. However, the molecular mechanism underlying the effect of nutrition-dependent states on odour-guided behaviours in hoverflies remains unclear. In this study, comparative transcriptome analysis of the antenna and proboscis from Eupeodes corollae under different feeding states was conducted. Compared with the previously published antennal transcriptome, a total of 32 novel chemosensory genes were identified, including 4 ionotropic receptors, 17 gustatory receptors, 9 odorant binding proteins and 2 chemosensory proteins. Analysis of differences in gene expression between different feeding states in male and female antennae and proboscises revealed that the expression levels of chemosensory genes were impacted by feeding state. For instance, the expression levels of EcorOBP19 in female antennae, EcorOBP6 in female proboscis, and EcorOR6, EcorOR14, EcorIR5 and EcorIR84a in male antennae were significantly upregulated after feeding. On the other hand, the expression levels of EcorCSP7 in male proboscis and EcorOR40 in male antennae were significantly downregulated. These findings suggest that nutritional state plays a role in the adaptation of hoverflies' olfactory system to food availability. Overall, our study provides important insights into the plasticity and adaptation of chemosensory systems in hoverflies.

昆虫的生理状态会影响其嗅觉系统。然而,营养依赖状态对食蚜蝇气味引导行为影响的分子机制仍不清楚。本研究对不同摄食状态下花冠蝶(Eupeodes corollae)的触角和长鼻进行了转录组比较分析。与之前发表的触角转录组相比,共发现了32个新的化感基因,包括4个离子受体、17个味觉受体、9个气味结合蛋白和2个化感蛋白。通过分析雌雄触角和长鼻在不同摄食状态下的基因表达差异,发现化学感觉基因的表达水平受摄食状态的影响。例如,雌性触角中的 EcorOBP19、雌性探针中的 EcorOBP6 以及雄性触角中的 EcorOR6、EcorOR14、EcorIR5 和 EcorIR84a 在摄食后的表达水平显著上调。另一方面,雄性探针中的 EcorCSP7 和雄性触角中的 EcorOR40 的表达水平则明显下调。这些发现表明,营养状态在食蚜蝇的嗅觉系统适应食物供应的过程中起着一定的作用。总之,我们的研究为研究食蚜蝇化学感觉系统的可塑性和适应性提供了重要的启示。
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引用次数: 0
Formation of amyloid fibrils by the regulatory 14-3-3ζ protein. 14-3-3ζ 蛋白调节淀粉样纤维的形成。
IF 5.8 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 Epub Date: 2024-01-17 DOI: 10.1098/rsob.230285
Darius Šulskis, Mantas Žiaunys, Andrius Sakalauskas, Rūta Sniečkutė, Vytautas Smirnovas

The 14-3-3 proteins are a highly conserved adaptor protein family with multi-layer functions, abundantly expressed in the brain. The 14-3-3 proteins modulate phosphorylation, regulate enzymatic activity and can act as chaperones. Most importantly, they play an important role in various neurodegenerative disorders due to their vast interaction partners. Particularly, the 14-3-3ζ isoform is known to co-localize in aggregation tangles in both Alzheimer's and Parkinson's diseases as a result of protein-protein interactions. These abnormal clumps consist of amyloid fibrils, insoluble aggregates, mainly formed by the amyloid-β, tau and α-synuclein proteins. However, the molecular basis of if and how 14-3-3ζ can aggregate into amyloid fibrils is unknown. In this study, we describe the formation of amyloid fibrils by 14-3-3ζ using a comprehensive approach that combines bioinformatic tools, amyloid-specific dye binding, secondary structure analysis and atomic force microscopy. The results presented herein characterize the amyloidogenic properties of 14-3-3ζ and imply that the well-folded protein undergoes aggregation to β-sheet-rich amyloid fibrils.

14-3-3 蛋白是一个高度保守的适配蛋白家族,具有多层功能,在大脑中大量表达。14-3-3 蛋白可调节磷酸化、调节酶活性并充当伴侣。最重要的是,由于它们有大量的相互作用伙伴,因此在各种神经退行性疾病中发挥着重要作用。特别是,在阿尔茨海默氏症和帕金森氏症中,14-3-3ζ异构体因蛋白质与蛋白质之间的相互作用而共同定位在聚集缠结中。这些异常团块由淀粉样纤维和不溶性聚集体组成,主要由淀粉样-β、tau 和 α-突触核蛋白形成。然而,14-3-3ζ是否以及如何聚集成淀粉样纤维的分子基础尚不清楚。在本研究中,我们采用一种综合方法,结合生物信息学工具、淀粉样蛋白特异性染料结合、二级结构分析和原子力显微镜,描述了 14-3-3ζ 形成淀粉样纤维的过程。本文介绍的结果描述了 14-3-3ζ 的淀粉样蛋白生成特性,并暗示这种折叠良好的蛋白质会聚集成富含 β 片的淀粉样纤维。
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引用次数: 0
Single-cell expression predicts neuron-specific protein homeostasis networks. 单细胞表达预测神经元特异性蛋白质平衡网络
IF 4.5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 Epub Date: 2024-01-24 DOI: 10.1098/rsob.230386
Sebastian Pechmann

The protein homeostasis network keeps proteins in their correct shapes and avoids unwanted aggregation. In turn, the accumulation of aberrantly misfolded proteins has been directly associated with the onset of ageing-associated neurodegenerative diseases such as Alzheimer's and Parkinson's. However, a detailed and rational understanding of how protein homeostasis is achieved in health, and how it can be targeted for therapeutic intervention in diseases remains missing. Here, large-scale single-cell expression data from the Allen Brain Map are analysed to investigate the transcription regulation of the core protein homeostasis network across the human brain. Remarkably, distinct expression profiles suggest specialized protein homeostasis networks with systematic adaptations in excitatory neurons, inhibitory neurons and non-neuronal cells. Moreover, several chaperones and Ubiquitin ligases are found transcriptionally coregulated with genes important for synapse formation and maintenance, thus linking protein homeostasis to the regulation of neuronal function. Finally, evolutionary analyses highlight the conservation of an elevated interaction density in the chaperone network, suggesting that one of the most exciting aspects of chaperone action may yet be discovered in their collective action at the systems level. More generally, our work highlights the power of computational analyses for breaking down complexity and gaining complementary insights into fundamental biological problems.

蛋白质平衡网络使蛋白质保持正确的形状,避免不必要的聚集。反过来,异常错误折叠蛋白质的积累与阿尔茨海默氏症和帕金森氏症等与衰老相关的神经退行性疾病的发病直接相关。然而,人们对健康状态下如何实现蛋白质平衡以及如何针对疾病进行治疗干预仍缺乏详细而合理的了解。本文分析了来自艾伦大脑图谱的大规模单细胞表达数据,以研究整个人类大脑核心蛋白质平衡网络的转录调控。值得注意的是,不同的表达谱表明,专门的蛋白质平衡网络在兴奋性神经元、抑制性神经元和非神经元细胞中具有系统的适应性。此外,还发现几种伴侣蛋白和泛素连接酶与突触形成和维持的重要基因存在转录核心关联,从而将蛋白质平衡与神经元功能调控联系起来。最后,进化分析强调了伴侣蛋白网络中相互作用密度升高的现象,这表明伴侣蛋白作用最令人兴奋的方面之一可能是它们在系统水平上的集体作用。更广泛地说,我们的工作凸显了计算分析在打破复杂性和获得对基本生物学问题的补充见解方面的力量。
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引用次数: 0
Significance of quantitative analyses of the impact of heterogeneity in mitochondrial content and shape on cell differentiation. 线粒体含量和形状异质性对细胞分化影响的定量分析意义重大。
IF 5.8 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 Epub Date: 2024-01-17 DOI: 10.1098/rsob.230279
Swati Agarwala, Sukhamoy Dhabal, Kasturi Mitra

Mitochondria, classically known as the powerhouse of cells, are unique double membrane-bound multifaceted organelles carrying a genome. Mitochondrial content varies between cell types and precisely doubles within cells during each proliferating cycle. Mitochondrial content also increases to a variable degree during cell differentiation triggered after exit from the proliferating cycle. The mitochondrial content is primarily maintained by the regulation of mitochondrial biogenesis, while damaged mitochondria are eliminated from the cells by mitophagy. In any cell with a given mitochondrial content, the steady-state mitochondrial number and shape are determined by a balance between mitochondrial fission and fusion processes. The increase in mitochondrial content and alteration in mitochondrial fission and fusion are causatively linked with the process of differentiation. Here, we critically review the quantitative aspects in the detection methods of mitochondrial content and shape. Thereafter, we quantitatively link these mitochondrial properties in differentiating cells and highlight the implications of such quantitative link on stem cell functionality. Finally, we discuss an example of cell size regulation predicted from quantitative analysis of mitochondrial shape and content. To highlight the significance of quantitative analyses of these mitochondrial properties, we propose three independent rationale based hypotheses and the relevant experimental designs to test them.

线粒体被称为细胞的 "动力之源",是携带基因组的独特双膜多层细胞器。线粒体的含量因细胞类型而异,在每个增殖周期中,细胞内的线粒体含量会精确地增加一倍。在脱离增殖周期后引发的细胞分化过程中,线粒体含量也会出现不同程度的增加。线粒体含量主要通过线粒体生物生成的调节来维持,而受损的线粒体则通过有丝分裂吞噬作用从细胞中清除。在任何线粒体含量一定的细胞中,稳态线粒体的数量和形状由线粒体裂变和融合过程之间的平衡决定。线粒体含量的增加以及线粒体裂变和融合过程的改变与细胞分化过程有着因果关系。在此,我们对线粒体含量和形状检测方法的定量方面进行了严格审查。之后,我们将这些线粒体特性与分化细胞进行定量联系,并强调这种定量联系对干细胞功能的影响。最后,我们讨论了一个通过线粒体形状和含量定量分析预测细胞大小调节的例子。为了强调对这些线粒体特性进行定量分析的意义,我们提出了三个独立的基于理性的假设以及检验这些假设的相关实验设计。
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引用次数: 0
2024: The quest continues. 2024: 探索仍在继续
IF 5.8 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 Epub Date: 2024-01-31 DOI: 10.1098/rsob.240010
Jonathon Pines
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引用次数: 0
IqgC is a potent regulator of macropinocytosis in the presence of NF1 and its loading to macropinosomes is dependent on RasG. 在 NF1 存在的情况下,IqgC 是大蛋白体吞噬作用的有效调节剂,其装入大蛋白体依赖于 RasG。
IF 4.5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 Epub Date: 2024-01-24 DOI: 10.1098/rsob.230372
Darija Putar, Anja Čizmar, Xiaoting Chao, Marija Šimić, Marko Šoštar, Tamara Ćutić, Lucija Mijanović, Ana Smolko, Hui Tu, Pierre Cosson, Igor Weber, Huaqing Cai, Vedrana Filić

RasG is a major regulator of macropinocytosis in Dictyostelium discoideum. Its activity is under the control of an IQGAP-related protein, IqgC, which acts as a RasG-specific GAP (GTPase activating protein). IqgC colocalizes with the active Ras at the macropinosome membrane during its formation and for some time after the cup closure. However, the loss of IqgC induces only a minor enhancement of fluid uptake in axenic cells that already lack another RasGAP, NF1. Here, we show that IqgC plays an important role in the regulation of macropinocytosis in the presence of NF1 by restricting the size of macropinosomes. We further provide evidence that interaction with RasG is indispensable for the recruitment of IqgC to forming macropinocytic cups. We also demonstrate that IqgC interacts with another small GTPase from the Ras superfamily, Rab5A, but is not a GAP for Rab5A. Since mammalian Rab5 plays a key role in early endosome maturation, we hypothesized that IqgC could be involved in macropinosome maturation via its interaction with Rab5A. Although an excessive amount of Rab5A reduces the RasGAP activity of IqgC in vitro and correlates with IqgC dissociation from endosomes in vivo, the physiological significance of the Rab5A-IqgC interaction remains elusive.

RasG 是盘基竹荪大核细胞增多的主要调节因子。它的活性受 IQGAP 相关蛋白 IqgC 的控制,IqgC 是 RasG 的特异性 GAP(GTPase 激活蛋白)。IqgC 在大体膜形成过程中和闭杯后的一段时间内与大体膜上的活性 Ras 共同定位。然而,在已经缺乏另一种 RasGAP--NF1--的轴突细胞中,失去 IqgC 只会引起液体吸收的轻微增强。在这里,我们证明了 IqgC 在 NF1 存在的情况下通过限制大体胞的大小在调节大体胞的过程中发挥了重要作用。我们进一步提供了证据,证明与 RasG 的相互作用是 IqgC 招募形成大核细胞杯所不可或缺的。我们还证明,IqgC 与 Ras 超家族的另一种小 GTP 酶 Rab5A 相互作用,但不是 Rab5A 的 GAP。由于哺乳动物的 Rab5 在早期内质体成熟过程中起着关键作用,我们推测 IqgC 可能通过与 Rab5A 的相互作用参与大内质体的成熟。虽然过量的 Rab5A 会降低 IqgC 在体外的 RasGAP 活性,并与 IqgC 在体内从内质体解离相关,但 Rab5A-IqgC 相互作用的生理意义仍然难以确定。
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引用次数: 0
Plant NLR immunity activation and execution: a biochemical perspective. 植物 NLR 免疫的激活和执行:生化视角。
IF 4.5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 Epub Date: 2024-01-24 DOI: 10.1098/rsob.230387
Federica Locci, Jane E Parker

Plants deploy cell-surface and intracellular receptors to detect pathogen attack and trigger innate immune responses. Inside host cells, families of nucleotide-binding/leucine-rich repeat (NLR) proteins serve as pathogen sensors or downstream mediators of immune defence outputs and cell death, which prevent disease. Established genetic underpinnings of NLR-mediated immunity revealed various strategies plants adopt to combat rapidly evolving microbial pathogens. The molecular mechanisms of NLR activation and signal transmission to components controlling immunity execution were less clear. Here, we review recent protein structural and biochemical insights to plant NLR sensor and signalling functions. When put together, the data show how different NLR families, whether sensors or signal transducers, converge on nucleotide-based second messengers and cellular calcium to confer immunity. Although pathogen-activated NLRs in plants engage plant-specific machineries to promote defence, comparisons with mammalian NLR immune receptor counterparts highlight some shared working principles for NLR immunity across kingdoms.

植物利用细胞表面和细胞内的受体来检测病原体的攻击并触发先天性免疫反应。在宿主细胞内,核苷酸结合/富亮氨酸重复(NLR)蛋白家族可作为病原体传感器或免疫防御输出和细胞死亡的下游介质,从而预防疾病。NLR 介导免疫的遗传基础已经确立,揭示了植物为对抗快速进化的微生物病原体而采取的各种策略。而 NLR 激活和信号传递到控制免疫执行元件的分子机制则不太清楚。在此,我们回顾了最近有关植物 NLR 传感器和信号功能的蛋白质结构和生物化学见解。这些数据表明,不同的 NLR 家族(无论是传感器还是信号转导器)如何汇聚到基于核苷酸的第二信使和细胞钙离子上,从而产生免疫力。虽然植物中病原体激活的 NLR 参与植物特有的机制以促进防御,但与哺乳动物 NLR 免疫受体对应物的比较突显了跨物种 NLR 免疫的一些共同工作原理。
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引用次数: 0
A critical threshold of MCM10 is required to maintain genome stability during differentiation of induced pluripotent stem cells into natural killer cells. 在诱导多能干细胞分化为自然杀伤细胞的过程中,需要一个临界阈值的 MCM10 来维持基因组的稳定性。
IF 4.5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 Epub Date: 2024-01-24 DOI: 10.1098/rsob.230407
Megan M Schmit, Ryan M Baxley, Liangjun Wang, Peter Hinderlie, Marissa Kaufman, Emily Simon, Anjali Raju, Jeffrey S Miller, Anja-Katrin Bielinsky

Natural killer (NK) cell deficiency (NKD) is a rare disease in which NK cell function is reduced, leaving affected individuals susceptible to repeated viral infections and cancer. Recently, a patient with NKD was identified carrying compound heterozygous variants of MCM10 (minichromosome maintenance protein 10), an essential gene required for DNA replication, that caused a significant decrease in the amount of functional MCM10. NKD in this patient presented as loss of functionally mature late-stage NK cells. To understand how MCM10 deficiency affects NK cell development, we generated MCM10 heterozygous (MCM10+/-) induced pluripotent stem cell (iPSC) lines. Analyses of these cell lines demonstrated that MCM10 was haploinsufficient, similar to results in other human cell lines. Reduced levels of MCM10 in mutant iPSCs was associated with impaired clonogenic survival and increased genomic instability, including micronuclei formation and telomere erosion. The severity of these phenotypes correlated with the extent of MCM10 depletion. Significantly, MCM10+/- iPSCs displayed defects in NK cell differentiation, exhibiting reduced yields of hematopoietic stem cells (HSCs). Although MCM10+/- HSCs were able to give rise to lymphoid progenitors, these did not generate mature NK cells. The lack of mature NK cells coincided with telomere erosion, suggesting that NKD caused by these MCM10 variants arose from the accumulation of genomic instability including degradation of chromosome ends.

自然杀伤(NK)细胞缺乏症(NKD)是一种罕见的疾病,NK 细胞功能降低,使患者容易反复感染病毒和患癌症。最近,发现一名 NKD 患者携带 DNA 复制所需的重要基因 MCM10(迷你染色体维护蛋白 10)的复合杂合变体,导致功能性 MCM10 的数量显著减少。该患者的 NKD 表现为功能成熟的晚期 NK 细胞丧失。为了了解 MCM10 缺乏如何影响 NK 细胞的发育,我们生成了 MCM10 杂合子(MCM10+/-)诱导多能干细胞(iPSC)系。对这些细胞系的分析表明,MCM10 是单倍体,这与其他人类细胞系的结果相似。突变型 iPSC 中 MCM10 水平的降低与克隆生成存活率受损和基因组不稳定性增加(包括微核形成和端粒侵蚀)有关。这些表型的严重程度与 MCM10 的消耗程度相关。值得注意的是,MCM10+/- iPSCs 显示出 NK 细胞分化缺陷,造血干细胞(HSCs)产量减少。虽然 MCM10+/- 造血干细胞能产生淋巴祖细胞,但这些祖细胞不能生成成熟的 NK 细胞。成熟 NK 细胞的缺乏与端粒侵蚀同时发生,这表明这些 MCM10 变体导致的 NKD 是由基因组不稳定性(包括染色体末端退化)的积累引起的。
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引用次数: 0
Human growth factor-mediated signalling through lipid rafts regulates stem cell proliferation, development and survival of Schistosoma mansoni. 人类生长因子通过脂质筏介导的信号调节曼氏血吸虫干细胞的增殖、发育和存活。
IF 5.8 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 Epub Date: 2024-01-10 DOI: 10.1098/rsob.230262
Shradha Maharjan, Ruth S Kirk, Scott P Lawton, Anthony J Walker

Although the mechanisms by which schistosomes grow and develop in humans are poorly defined, their unique outer tegument layer, which interfaces with host blood, is considered vital to homeostasis of the parasite. Here, we investigated the importance of tegument lipid rafts to the biology of Schistosoma mansoni in the context of host-parasite interactions. We demonstrate the temporal clustering of lipid rafts in response to human epidermal growth factor (EGF) during early somule development, concomitant with the localization of anteriorly orientated EGF receptors (EGFRs) and insulin receptors, mapped using fluorescent EGF/insulin ligand. Methyl-β-cyclodextrin (MβCD)-mediated depletion of cholesterol from lipid rafts abrogated the EGFR/IR binding at the parasite surface and led to modulation of protein kinase C, extracellular signal-regulated kinase, p38 mitogen-activated protein kinase and Akt signalling pathways within the parasite. Furthermore, MβCD-mediated lipid raft disruption, and blockade of EGFRs using canertinib, profoundly reduced somule motility and survival, and attenuated stem cell proliferation and somule growth and development particularly to the fast-growing liver stage. These findings provide a novel paradigm for schistosome development and vitality in the host, driven through host-parasite interactions at the tegument, that might be exploitable for developing innovative therapeutic approaches to combat human schistosomiasis.

尽管血吸虫在人体内生长发育的机制尚不十分明确,但其独特的外被膜层与宿主的血液相接,被认为对寄生虫的平衡至关重要。在这里,我们研究了在宿主与寄生虫相互作用的背景下,外膜脂筏对曼氏血吸虫生物学的重要性。我们利用荧光 EGF/胰岛素配体绘制了胰岛素受体(EGFR)和胰岛素受体的定位图。甲基-β-环糊精(MβCD)介导的脂质筏胆固醇耗竭削弱了寄生虫表面的表皮生长因子受体/胰岛素受体结合,并导致寄生虫体内蛋白激酶C、细胞外信号调节激酶、p38丝裂原活化蛋白激酶和Akt信号通路的调节。此外,MβCD介导的脂质筏破坏和使用卡奈替尼阻断表皮生长因子受体可显著降低体细胞的运动性和存活率,并抑制干细胞增殖和体细胞的生长发育,尤其是在快速生长的肝脏阶段。这些发现为血吸虫在宿主体内的发育和活力提供了一种新的范式,这种范式是通过宿主与寄生虫在表皮的相互作用驱动的,可用于开发防治人类血吸虫病的创新治疗方法。
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引用次数: 0
Deficiency of the ribosomal protein uS10 (RPS20) reorganizes human cells translatome according to the abundance, CDS length and GC content of mRNAs. 核糖体蛋白uS10(RPS20)的缺乏会根据mRNA的丰度、CDS长度和GC含量重组人体细胞的翻译组。
IF 4.5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 Epub Date: 2024-01-31 DOI: 10.1098/rsob.230366
Yueming Tian, Elena S Babaylova, Alexander V Gopanenko, Alexey E Tupikin, Marsel R Kabilov, Alexey A Malygin

Ribosomal protein uS10, a product of the RPS20 gene, is an essential constituent of the small (40S) subunit of the human ribosome. Disruptive mutations in its gene are associated with a predisposition to hereditary colorectal carcinoma. Here, using HEK293T cells, we show that a deficiency of this protein leads to a decrease in the level of ribosomes (ribosomal shortage). RNA sequencing of the total and polysome-associated mRNA samples reveals hundreds of genes differentially expressed in the transcriptome (t)DEGs and translatome (p)DEGs under conditions of uS10 deficiency. We demonstrate that the (t)DEG and (p)DEG sets partially overlap, determine genes with altered translational efficiency (TE) and identify cellular processes affected by uS10 deficiency-induced ribosomal shortage. We reveal that translated mRNAs of upregulated (p)DEGs and genes with altered TE in uS10-deficient cells are generally more abundant and that their GC contents are significantly lower than those of the respective downregulated sets. We also observed that upregulated (p)DEGs have longer coding sequences. Based on our findings, we propose a combinatorial model describing the process of reorganization of mRNA translation under conditions of ribosomal shortage. Our results reveal rules according to which ribosomal shortage reorganizes the transcriptome and translatome repertoires of actively proliferating cells.

核糖体蛋白 uS10 是 RPS20 基因的产物,是人类核糖体小亚基(40S)的重要组成部分。其基因的破坏性突变与遗传性结直肠癌的易感性有关。在这里,我们利用 HEK293T 细胞证明,缺乏这种蛋白质会导致核糖体水平下降(核糖体缺乏)。总mRNA和多聚体相关mRNA样本的RNA测序显示,在uS10缺乏的条件下,数百个基因在转录组(t)DEG和转译组(p)DEG中差异表达。我们证明(t)DEG和(p)DEG集部分重叠,确定了翻译效率(TE)改变的基因,并确定了受uS10缺乏引起的核糖体短缺影响的细胞过程。我们发现,在uS10缺乏的细胞中,上调(p)DEG和TE改变的基因的翻译mRNA通常更丰富,其GC含量明显低于相应的下调集。我们还观察到,上调(p)DEGs 的编码序列较长。根据我们的研究结果,我们提出了一个组合模型,描述了核糖体短缺条件下 mRNA 翻译的重组过程。我们的研究结果揭示了核糖体短缺重组活跃增殖细胞转录组和翻译组的规则。
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引用次数: 0
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