Pakistan Journal of Biological Sciences最新文献

Multidrug-resistant (MDR) bacteria have risen alarmingly in the last few decades, posing a serious threat to human health. The need for effective bacterial resistance treatment is urgent and unmet due to the rise in morbidity and mortality that has coincided with the prevalence of infections caused by MDR bacteria. Using its creative and unconventional methods, effective antibiotics for MDR bacteria could be developed using nanomedicine techniques. To combat microbial resistance, a number of strategies have been developed, including the use of natural bactericides, the introduction of fresh antibiotics, the application of combination therapy and the creation of NP-based antibiotic nanocarriers. The absence of novel antibacterial agents has worsened the situation for MDR bacteria. Ineffective antibiotics used to treat MDR bacteria also contribute to the bacteria's tolerance growing. Nanoparticles (NPs) are the most efficient method for eliminating MDR bacteria because they serve as both carriers of natural antibiotics and antimicrobials and active agents against bacteria. Additionally, surface engineering of nanocarriers has important benefits for focusing on and modifying a variety of resistance mechanisms. The use of nanocarrier systems in drug delivery for overcoming bacterial resistance is covered in this review along with various mechanisms of antibiotic resistance.

<b>Background and Objective:</b> Belawan River is located in Deli Serdang and Medan Districts, North Sumatra, Indonesia. Belawan River passes through residential areas, industry, steam power plants (PLTU), PDAM and encroachment. Waste is directly discharged into water bodies due to many human activities, so the quality of water and aquatic biota is disturbed. Phytoplankton diversity is influenced by water quality conditions because its existence depends heavily on the water conditions of the Belawan River. Analyzing the model of phytoplankton diversity in the Belawan River was the purpose of this study. <b>Materials and Methods:</b> The analysis was conducted in the form of phytoplankton diversity by obtaining phytoplankton species and their distribution and analyze the environment such as the water quality of the Belawan River. Sampling was taken by purposive random method with 5 different locations with 3 times the test with, namely starting from the upstream, middle and downstream Belawan River area in 2010, 2015 and 2020. Stages of research methods, obtained types of plankton, abundance (A), relative abundance (RA), presence frequency (PF), equitability (E) and diversity (H) and analysis of water quality (temperature, light penetration, light intensity, depth, current speed, salinity, pH, DO, oxygen saturation percent, BOD and COD). <b>Results:</b> Twenty three genera of phytoplankton were found in 2020. Phytoplankton abundance was highest in the <i>Chaetoceros</i> sp., genera with an abundance of 186 ind m² at station 5. Diversity (H) was highest at station 5 at 2.30 and lowest at station 5 at 1.87. Phytoplankton in five stations was relatively low. The DO has a very strong effect on phytoplankton's diversity. The model of phytoplankton diversity relationship with water quality is y = -2.09235475-0.283821248x₁-0.000034042331x₂²-0.000000317192297x₃²+ 0.0000138747473x₄²-0.0642412267x₅²+0.0436398590x₆-0.0107999363x₇²+0.0000469016376x₈². <b>Conclusion:</b> Dissolved oxygen (DO) has an effect on the diversity of phytoplankton in the Belawan River where the highest phytoplankton is <i>Chaetoceros</i> sp., of 23 genera which was found with a diversity value of 2.30 at station V.

<b>Background and Objective:</b> Hyperuricemia is a disease triggered by disorders of uric acid metabolism. Therefore, this study evaluated the potential of leaves ethanolic extract of <i>Etlingera hemisphaerica</i> (LE3H) and fruits ethanolic extract <i>E. hemisphaerica</i> (FE3H) to restore hyperuricemia in mice. <b>Materials and Methods:</b> Six groups (A0, A1, A2, A3, A4 and A5) each consisted of four male mice. Hyperuricemia in mice was induced by giving 0.3 mL of fresh chicken liver juice (FCLJ) for seven days (A1, A2, A3, A4 and A5). The condition of hyperuricemia in A1 is not neutralized. Meanwhile, hyperuricemia conditions in A2, A3, A4 and A5 were neutralized for seven days by giving 0.01 mg g¹ body weight (BW) allopurinol, 0.13, 0.26 and 0.36 mg g¹ BW LE3H. The control group (A0) only received double-distilled water in the same way. Blood uric acid levels were measured with the GCU Meter Device before and after the induction of hyperuricemia and after efforts to neutralize the hyperuricemia condition. Six groups (B0, B1, B2, B3, B4, B5) each consisting of four male mice were also provided to test the potential of FE3H. The recovery potential FE3H against hyperuricemia was tested separately in the same way as was done for LE3H. <b>Results:</b> Giving FCLJ significantly increased (140.00-187.00%) uric acid compared to the control, so hyperuricemia was achieved. Doses of 0.13, 0.26 and 0.36 mg g¹ BW LE3H significantly recovered hyperuricemia as much as 54.09, 56.14 and 60.88%, respectively. Meanwhile, doses of 0.13, 0.26 and 0.36 mg g¹ FE3H significantly recovered hyperuricemia as much as 60.37, 62.24 and 65.572%, respectively. The LE3H and FE3H at the same dose showed that FE3H had a higher potential to restore hyperuricemia than LE3H. <b>Conclusion:</b> Leave and fruit ethanolic extract of <i>E. hemisphaerica</i> can potentially restore hyperuricemia in mice.

<b>Background and Objective:</b> <i>Nepenthes</i> is a marsupial plant that is a modification of the leaf tip and serves to trap insects. The difference in the position of the pitcher is expected to distinguish the insects that inhabit it. One of the locations <i>Nepenthes</i> was Gunung Kunyit which is included in the Kerinci Seblat National Park Area. Insects trapped in the <i>Nepenthes</i> can be studied through taxonomic studies. The objectives of this study were to determine the diversity of insects trapped in the <i>Nepenthes</i> and to know the species of <i>Nepenthes.</i> <b>Materials and Methods:</b> The research was conducted from February to May, 2022. The study used a survey method by direct observation in the field. <b>Results:</b> The insects found in the <i>Nepenthes</i> consisted of 3 orders, 7 families and 10 species. The most insects found to be <i>Crematogaster</i> sp., (82 individuals) and the least insects <i>Sceliphron</i> sp. and <i>Dasiops</i> sp. (5 individuals). At the research site, three species of <i>Nepenthes</i>, <i>N. bongso</i>, <i>N. gymnamphora</i> and <i>N. inermis</i> at an altitude of 1,768-1,993 meters above sea level (m.a.s.l.). <b>Conclusion:</b> The insect diversity index in the upper pitcher was 1.79 and for the lower pitcher position was 1.31, so it was categorized as a medium level.

<b>Background and Objective:</b> Type 2 L-asparaginase enzyme can be used as a cancer therapy agent and prevent acrylamide formation in food products. Enzymes produced by thermohalophilic bacteria can provide high activity at high temperatures so they are needed on an industrial scale. Hence, this study aims to determine the characteristics of the gene encoding type 2 L-asparaginase enzyme in the thermohalophilic bacterial isolate CAT3.4. <b>Materials and Methods:</b> This research is a type of exploratory research. The characteristics of the gene encoding type 2 L-asparaginase were determined using the PCR technique using the primer pairs AsnBac2-F2 (5'-CTCACGGGAATCTCCATAACTC-3') and AsnBac2-R2 (5'CAGCGATGTAACAGACAGCATC-3'). The characterization process was carried out in stages: Isolation of genomic DNA using a modified alkali-lysis method, nucleotide and protein similarity analysis using BLASTn analysis on the NCBI website, construction of a phylogenetic tree using the MEGAX program, restriction enzyme mapping and amino acid analysis using the Bioedit program. <b>Results:</b> The characterization results showed that the PCR product has a size of 1594 bp with a CDS of 1128 bp, has a similarity value of 100% with <i>Bacillus subtilis</i>, has seven restriction enzymes as molecular markers for the type 2 L-asparaginase gene at the species level: <i>Bsr</i>GI, <i>Dra</i>I, <i>Eco</i>RV, <i>Hind</i>III, <i>Hpy</i>CH4IV , <i>Ssp</i>I and <i>Tai</i>I, have dominant hydrophilic regions and are in the same subclass as <i>Bacillus subtilis</i> strain GOT9. <b>Conclusion:</b> The target gene was similar to the gene encoding type 2 L-asparaginase from <i>Bacillus subtilis</i> with a max identity of 98.85%, query coverage value of 100% and E-value of 0.

<b>Background and Objective:</b> Infertility is still a phenomenon in the community, so consuming Moringa leaves (<i>Moringa oleifera</i> Lam.) is expected to increase fertility. This study aimed to determine the effect of Moringa leaf extract (<i>Moringa oleifera</i> Lam.) on the diameter of the primary and secondary follicles in female mice (<i>Mus musculus</i>). <b>Materials and Methods:</b> This study was an experiment using a Completely Randomized Design (CRD). The population of this study was 45 mice and samples were obtained by a simple random sampling technique from as many as 24 mice with the following criteria: Weight 20-25 g, 2-3 months old, female and in good health. Data analysis was performed through the ANOVA Test with a confidence level of α = 0.05 and further tested for the least significant difference (LSD). <b>Results:</b> Moringa leaf extract significantly positively affects the diameter of primary and secondary follicles in female mice (p<0.05). The average primary follicle diameter was P₀ (92.65 μm), P₁ (124.92 μm), P₂(150.72 μm), P₃ (175.68 μm) and the average secondary follicle diameter was control (157.17 μm), P₁(171.33 μm), P₂ (204.57 μm), P₃ (211.11 μm). Giving Moringa leaf extract (<i>Moringa oleifera</i> Lam.) significantly increases the diameter of mice's primary and secondary follicles due to the presence of vitamin E in Moringa leaf extract (<i>Moringa oleifera</i> Lam.). <b>Conclusion:</b> This can stimulate granulosa cells to secrete the hormone estrogen, causing an increase in the diameter of the primary and secondary follicles.

The DNA is constantly under attack from endogenous and exogenous damaging agents. The damaged DNA must be repaired quickly to avoid genomic instability and to prevent the occurrence of a malignant transformation. Once a lesion is detected, the DNA repair mechanism initiates and replaces the structurally altered base or any other abnormality. The cell repair mechanisms include direct reversal, excision repair (base excision repair [BER] and nucleotide excision repair [NER]), mismatch repair (MMR), homologous recombination repair (HR) and non-homologous end joining (NHEJ). Unrepaired DNA could lead to mutation, cell death or cancer. This review will discuss how the defects in DNA repair play a vital role in cancer initiation, development and progression.

<b>Background and Objective:</b> Saburai goat (<i>Capra hircus</i>) is a crossbred goat from Boer buck (75%) and Ettawa doe (25%) for meat production purposes. This study was carried out to detect the mutation points or Single Nucleotide Polymorphisms (SNPs) in the myostatin (<i>MSTN</i>) gene of Saburai with the forward sequencing method. <b>Materials and Methods:</b> A total of twenty-one blood samples of Saburai does (75% Boer, 25% Ettawa) were collected from the Villager Breeder Center (VBC) at Tanggamus Regency of Lampung Province, Indonesia. The DNA analysis consists of DNA isolation, PCR analysis and sequencing analysis. The record data were used for association study with the mathematical model: Y_ij = μ+G_i+Є_ij. <b>Results:</b> Research showed that one common SNP of g.217_218.indel.TTTTA (5'UTR) and three novel SNPs of c.386G>C (exon 1), g.641_642.indel.T (intron 2) and c.4957G>C (exon 3) were detected in the present study. In this study, a novel SNP on exon 1 and intron 2 of Saburai <i>MSTN</i> gene has a moderate PIC value (>0.30). In addition, a novel SNP on exon 1 and exon 3 of the Saburai <i>MSTN</i> gene was detected as a missense mutation of A55P and A43P, respectively. Goats with the heterozygous genotype have higher growth traits compared to goats with the homozygous genotype. <b>Conclusion:</b> The goats with heterozygous genotypes can be further developed to increase the productivity of Saburai goats.

<b>Background and Objective:</b> The flavor enhancer Monosodium Glutamate (MSG) is mostly utilized in Asian and West African cuisines, especially in West African and Asian dishes. However, due to its availability, largely without labeling, in many food products, unintentional overuse of this food additive may occur. The objective of this study was to find out how selenium nanoparticles affected the toxicity of MSG in male albino rats' testicles. <b>Materials and Methods:</b> As 35 Wistar male rats partitioned into 5 groups: G1: Control rats, G2: Received Se-NPs at 0.4 mg kg¹ b.wt., orally, G3: Injected with MSG at a daily dose of 4 g kg¹ b.wt., intraperitoneally (IP), G4: Ingested a daily oral dose of Se-NPs for 7 successive days and on the 7th day, received the first dose of MSG IP 4 g kg¹ b.wt., then received both treatments till the end of the study and G5: Administered a daily oral dose of 4 g kg¹ MSG, followed by Se-NPs at a daily dose of 0.4 mg kg¹ b.wt., the experiment continued for 28 days. Serum testosterone hormone, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), the levels of serum lipid peroxidation (MDA), reduced Glutathione (GSH), Glutathione Peroxidase (GSH-Px), superoxide dismutase (SOD) and Lactate Dehydrogenase (LDH) were estimated and samples from testis were separated for histological analysis. <b>Results:</b> The MSG treatment induced a significant decline in the values of serum testosterone, FSH, LH, GSH, GSH-Px and SOD. It also increased the values of serum MDA and LDH and spermatic arrest. While, the administration of Se-NPs orally before MSG treatment resulted in a decline in the values of serum MDA and LDH, an elevation in the values of serum GSH, GSH-PX and SOD, testosterone, FSH, LH and reappearance of sperm. <b>Conclusion:</b> The use of Se-NPs as a protector exhibited more improvement in values of estimated hormones and oxidative stress markers than using it as a therapy.