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M protein of type 12 Strepto coccus pyogenes. Isolation by electrofocusing and some molecular weight-dependent properties. 12型化脓性链球菌M蛋白。电聚焦分离和一些分子量依赖性质。
Pathologia et microbiologia
Pub Date : 1975-01-01
J Havlícek

A method for the isolation and purification of M protein was developed. Purified cell walls were sonically disrupted, solubilized M protein was precipitated by ammonium sulphate and then electrofocused. Both in this material and in hot acid extracts type-specific trypsin-sensitive antigens with two separately precipitating moieties were found. Evidence is adduced showing that they both belong to the M protein complex. The molecular weight of our purified M protein ranged between 400,000 and 20,000 daltons, giving a peak at 150,000 daltons. The pI of this material was found to be 5.4-5.6. There were marked differences between the behaviour of the low, medium and high molecular weight fractions obtained from purified M protein by gel filtration.

建立了一种分离纯化M蛋白的方法。纯化后的细胞壁被声波破坏，溶解后的M蛋白用硫酸铵沉淀，然后电聚焦。在该材料和热酸提取物中都发现了具有两个单独沉淀部分的类型特异性胰蛋白酶敏感抗原。证据表明它们都属于M蛋白复合物。我们纯化的M蛋白分子量在400,000到20,000道尔顿之间，峰值为150,000道尔顿。这个材料的pI是5.4-5.6。凝胶过滤得到的M蛋白低、中、高分子量组分的行为有显著差异。
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引用次数: 0
M protein of type 12 Strepto coccus pyogenes. Isolation by electrofocusing and some molecular weight-dependent properties. 12型化脓性链球菌M蛋白。电聚焦分离和一些分子量依赖性质。
Pathologia et microbiologia
Pub Date : 1975-01-01 DOI: 10.1159/000162735
Jircaron;í Havlíček
A method for the isolation and purification of M protein was developed. Purified cell walls were sonically disrupted, solubilized M protein was precipitated by ammonium sulphate and then electrofocused. Both in this material and in hot acid extracts type-specific trypsin-sensitive antigens with two separately precipitating moieties were found. Evidence is adduced showing that they both belong to the M protein complex. The molecular weight of our purified M protein ranged between 400,000 and 20,000 daltons, giving a peak at 150,000 daltons. The pI of this material was found to be 5.4-5.6. There were marked differences between the behaviour of the low, medium and high molecular weight fractions obtained from purified M protein by gel filtration.
建立了一种分离纯化M蛋白的方法。纯化后的细胞壁被声波破坏，溶解后的M蛋白用硫酸铵沉淀，然后电聚焦。在该材料和热酸提取物中都发现了具有两个单独沉淀部分的类型特异性胰蛋白酶敏感抗原。证据表明它们都属于M蛋白复合物。我们纯化的M蛋白分子量在400,000到20,000道尔顿之间，峰值为150,000道尔顿。这个材料的pI是5.4-5.6。凝胶过滤得到的M蛋白低、中、高分子量组分的行为有显著差异。
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引用次数: 8
Unexplained pulmonary hypertension. 原因不明的肺动脉高压。
Pathologia et microbiologia
Pub Date : 1975-01-01 DOI: 10.1159/000162829
C. Wagenvoort
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引用次数: 8
Transplantation arteriopathy. 移植动脉病。
Pathologia et microbiologia
Pub Date : 1975-01-01 DOI: 10.1159/000162823
M J Mihatsch, H U Zollinger, F Gudat, J Schuppler, U N Riede, G Thiel, F Brunner, F Enderlin
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引用次数: 2
Recovery of a new herpesvirus from the ground squirrel (Citellus citellus). 从地松鼠(Citellus Citellus)中恢复一种新的疱疹病毒。
Pathologia et microbiologia
Pub Date : 1975-01-01 DOI: 10.1159/000162720
P Diosi, L Plavosin, P Arcan, C David

A new herpesvirus recovered from the kidneys of mature ground squirrels (Citellus citellus) produced an effect in tissue culture cells resembling that of herpesviruses, including the formation of multinucleated syncytia and type A intranuclear inclusions. The isolate caused latent infection in the natural host, but it proved fatal for intracerebrally inoculated suckling mice and it produced pocks on the chorioallantoic membrane of embryonated eggs. Electron-microscopic examination of infected cells revealed intranuclear virus particles exhibiting a size and ultrastructure characteristic of herpesviruses. The isolate was ether-resistant and a DNA nucleic acid type was inferred from observations on inhibition by fluorinated pyrimidine. This ground squirrel herpesvirus was found to be antigenically distinct from Herpesvirus hominis and from ground squirrel cytomegalovirus, with no detectable cross-reactivity.

从成熟地松鼠肾脏中提取的一种新的疱疹病毒(黄鼠)在组织培养细胞中产生类似疱疹病毒的作用，包括形成多核合胞体和A型核内包涵体。该分离物在自然宿主中引起潜伏感染，但对经脑内接种的乳鼠是致命的，并且在胚胎卵的绒毛膜尿囊膜上产生小孔。感染细胞的电镜检查显示核内病毒颗粒具有疱疹病毒的大小和超微结构特征。该分离物具有醚抗性，通过观察氟化嘧啶的抑制作用推断为DNA核酸型。这种地鼠疱疹病毒在抗原性上与人疱疹病毒和地鼠巨细胞病毒不同，没有可检测到的交叉反应性。
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引用次数: 7
Effects of Newcastle disease virus (NDV) on infection of adult mice with the Thogoto-like Ar-126 arbovirus. 新城疫病毒(NDV)对成年小鼠感染thogoto样Ar-126虫媒病毒的影响。
Pathologia et microbiologia
Pub Date : 1975-01-01 DOI: 10.1159/000162842
A Salerno, M Albanese, P Byfield

Inoculation of mice with Newcastle disease virus (NDV) alters the course of infection with the Thogoto-like arbovirus Tho-Ar-126. The Tho-Ar-126 content of liver, spleen, and lymph nodes was approximately 10 times greater in mice treated with NDV 24 h before infection; the mortality was somewhat increased, but liver damage (as indicated by serum transaminase levels) did not seem to be potentiated. Lymphocytopenia was observed in NDV-inoculated mice, and in the spleen and lymph nodes the proportion of lymphocytes susceptible to lysis by anti-theta (a marker for thymus-derived lymphocytes) was markedly decreased in these animals. This suggests that NDV potentiates infection by Tho-Ar-126 through its action on thymus-derived lymphocytes.

用新城疫病毒(NDV)接种小鼠可改变猪样虫媒病毒(thogoto - ar -126)的感染过程。在感染前24 h，新城疫小鼠肝脏、脾脏和淋巴结中Tho-Ar-126的含量增加了约10倍;死亡率有所增加，但肝损害(由血清转氨酶水平显示)似乎没有增强。在ndv接种小鼠中观察到淋巴细胞减少，脾脏和淋巴结中抗- θ(胸腺源淋巴细胞的标记物)易溶解的淋巴细胞比例明显减少。这表明NDV通过对胸腺源性淋巴细胞的作用增强了Tho-Ar-126的感染。
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引用次数: 1
[Proceedings: Histopathology and immunofluorescence of myxovirus hepatitis in sensitive and genetically resistant mice]. [论文集:敏感和基因抗性小鼠黏液病毒性肝炎的组织病理学和免疫荧光研究]。
Pathologia et microbiologia
Pub Date : 1975-01-01
H Arnheiter, O Haller
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引用次数: 0
[Proceedings: Comparative study of chloramphenicol acetyltransferases in salmonellae]. [论文集:沙门氏菌中氯霉素乙酰转移酶的比较研究]。
Pathologia et microbiologia
Pub Date : 1975-01-01
M A Charpié, J S Pitton
{"title":"[Proceedings: Comparative study of chloramphenicol acetyltransferases in salmonellae].","authors":"M A Charpié,&nbsp;J S Pitton","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":19854,"journal":{"name":"Pathologia et microbiologia","volume":"42 4","pages":"230-1"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12405578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Proceedings: Early identification of antibiotics in a screening program using streptothricin as an example]. [论文集:以链霉素为例筛选程序中抗生素的早期识别]。
Pathologia et microbiologia
Pub Date : 1975-01-01
W Pache
{"title":"[Proceedings: Early identification of antibiotics in a screening program using streptothricin as an example].","authors":"W Pache","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":19854,"journal":{"name":"Pathologia et microbiologia","volume":"42 4","pages":"243-4"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12405580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Endarteritis obliterans or arteriosclerosis obliterans. 闭塞性终动脉炎或闭塞性动脉硬化。
Pathologia et microbiologia
Pub Date : 1975-01-01
A Kádár, H Jellinek, Z Béres
{"title":"Endarteritis obliterans or arteriosclerosis obliterans.","authors":"A Kádár,&nbsp;H Jellinek,&nbsp;Z Béres","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":19854,"journal":{"name":"Pathologia et microbiologia","volume":"43 2-O","pages":"167-72"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12405591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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