Keito Fukushima, Takeshi Yabutsuka, S. Takai, T. Yao
: We made micropores on the surface of a polyether ether ketone (PEEK) by immersing in sulfuric acid. In order to provide bioactivity to PEEK, we treated the surfaces of the specimens with glow-discharge in O 2 gas atmosphere and precipitated Apatite Nucleus (AN) in the micropores. We evaluated apatite-forming ability of the specimens by using SBF and measured adhesive strength of formed apatite layer. In this study, we researched which treatment is effective to give bioactivity to PEEK.
{"title":"INVESTIGATION OF EFFECTIVE PROCEDURES IN FABRICATION OF BIOACTIVE PEEK USING THE FUNCTION OF APATITE NUCLEI","authors":"Keito Fukushima, Takeshi Yabutsuka, S. Takai, T. Yao","doi":"10.3363/PRB.31.31","DOIUrl":"https://doi.org/10.3363/PRB.31.31","url":null,"abstract":": We made micropores on the surface of a polyether ether ketone (PEEK) by immersing in sulfuric acid. In order to provide bioactivity to PEEK, we treated the surfaces of the specimens with glow-discharge in O 2 gas atmosphere and precipitated Apatite Nucleus (AN) in the micropores. We evaluated apatite-forming ability of the specimens by using SBF and measured adhesive strength of formed apatite layer. In this study, we researched which treatment is effective to give bioactivity to PEEK.","PeriodicalId":20022,"journal":{"name":"Phosphorus Research Bulletin","volume":"35 1","pages":"31-37"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81233132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hiroki Nakamura, Michiaki Sakaguchi, Takeshi Yabutsuka, S. Takai, T. Yao
: By raising the temperature or the pH of an acellular simulated body fluid (SBF), nanoparticles of amorphous calcium phosphate are precipitated. The nanoparticles are highly active to induce hydroxyapatite formation in body fluid or SBF. In this study, the authors fabricated hydroxyapatite microcapsules encapsulating an agarose gel by using the nanoparticles and examined the effects of the pH and the concentrations of SBF on the microcapsules formation process. Increase in ion concentrations or raising pH of SBF caused the acceleration of the microcapsules formation. (Received
{"title":"THE EFFECTS OF SBF CONDITIONS ON ENCAPSULATION OF AGAROSE GEL WITH HYDROXYAPATITE MICROCAPSULES","authors":"Hiroki Nakamura, Michiaki Sakaguchi, Takeshi Yabutsuka, S. Takai, T. Yao","doi":"10.3363/PRB.31.9","DOIUrl":"https://doi.org/10.3363/PRB.31.9","url":null,"abstract":": By raising the temperature or the pH of an acellular simulated body fluid (SBF), nanoparticles of amorphous calcium phosphate are precipitated. The nanoparticles are highly active to induce hydroxyapatite formation in body fluid or SBF. In this study, the authors fabricated hydroxyapatite microcapsules encapsulating an agarose gel by using the nanoparticles and examined the effects of the pH and the concentrations of SBF on the microcapsules formation process. Increase in ion concentrations or raising pH of SBF caused the acceleration of the microcapsules formation. (Received","PeriodicalId":20022,"journal":{"name":"Phosphorus Research Bulletin","volume":"3 1","pages":"9-14"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83384775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: Lower phosphates, such as phosphites and hypo-phosphites, are known to have a characteristic of particularly strong reducibility. We researched on phosphite compounds and found out that aluminum phosphite had an excellent property. Aluminum phosphite, the product name of ‘APA-100’, was investigated in this study. Since APA-100 has a characteristic of hardened foam material by heating, this material is effective as a flame retardant. As a result of corrosion-resistant evaluation using the copper foil, the control of the corrosion by the effect of APA-100 was found out. Furthermore, we succeeded in the development of the fine particle type of APA-100. The details on aluminum phosphite (APA-100) for various uses were reported.
{"title":"DEVELOPMENT OF ALUMINUM PHOSPHITE AND ITS APPLICATION","authors":"R. Oyama, Hiroyuki Shimomichi, Tomofumi Yamanaka","doi":"10.3363/PRB.32.10","DOIUrl":"https://doi.org/10.3363/PRB.32.10","url":null,"abstract":": Lower phosphates, such as phosphites and hypo-phosphites, are known to have a characteristic of particularly strong reducibility. We researched on phosphite compounds and found out that aluminum phosphite had an excellent property. Aluminum phosphite, the product name of ‘APA-100’, was investigated in this study. Since APA-100 has a characteristic of hardened foam material by heating, this material is effective as a flame retardant. As a result of corrosion-resistant evaluation using the copper foil, the control of the corrosion by the effect of APA-100 was found out. Furthermore, we succeeded in the development of the fine particle type of APA-100. The details on aluminum phosphite (APA-100) for various uses were reported.","PeriodicalId":20022,"journal":{"name":"Phosphorus Research Bulletin","volume":"327 1","pages":"10-13"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80402285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Sugo, K. Uchida, Kouji Naruse, M. Uchino, N. Hirakawa, M. Toyama, Genyo Miyajima, S. Ikeda, K. Urabe
: We previously reported that the antibiotic elution profiles from calcium phosphate cement (CPC) vary depending on the type and number of impregnated antibiotics. Here, we attempted to determine the underlying factors affecting the elution of vancomycin (VCM) and gentamicin (GM) from CPC. Changes in pore size were investigated after the impregnation of CPC with various antibiotics in either the liquid or powdered state. Porosimetry analysis revealed that the pore sizes of CPC impregnated with powdered antibiotic were larger than those of CPC impregnated with liquid antibiotic. As powdered VCM had poor solubility in the CPC solvent, insoluble VCM particles would block the crystal growth. Scanning electron microscopy observation demonstrated that the solubility of VCM increased after the impregnation of CPC with GM. These findings suggest that the size of CPC pores, which act as passages for antibiotic elution, are affected by the size of undissolved antibiotic particles present within CPC.
{"title":"ELUTION MECHANISM OF VANCOMYCIN AND GENTAMICIN FROM CALCIUM PHOSPHATE CEMENT","authors":"K. Sugo, K. Uchida, Kouji Naruse, M. Uchino, N. Hirakawa, M. Toyama, Genyo Miyajima, S. Ikeda, K. Urabe","doi":"10.3363/PRB.32.1","DOIUrl":"https://doi.org/10.3363/PRB.32.1","url":null,"abstract":": We previously reported that the antibiotic elution profiles from calcium phosphate cement (CPC) vary depending on the type and number of impregnated antibiotics. Here, we attempted to determine the underlying factors affecting the elution of vancomycin (VCM) and gentamicin (GM) from CPC. Changes in pore size were investigated after the impregnation of CPC with various antibiotics in either the liquid or powdered state. Porosimetry analysis revealed that the pore sizes of CPC impregnated with powdered antibiotic were larger than those of CPC impregnated with liquid antibiotic. As powdered VCM had poor solubility in the CPC solvent, insoluble VCM particles would block the crystal growth. Scanning electron microscopy observation demonstrated that the solubility of VCM increased after the impregnation of CPC with GM. These findings suggest that the size of CPC pores, which act as passages for antibiotic elution, are affected by the size of undissolved antibiotic particles present within CPC.","PeriodicalId":20022,"journal":{"name":"Phosphorus Research Bulletin","volume":"267 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76278967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amorphous calcium phosphate (ACP) is a known precursor of apatite, which is one of the main components of human bone. Synthesized ACP transforms into low-crystallinity hydroxyapatite (HAp) around body temperature. Thus, if low-crystallinity calcium phosphate cement, containing active ingredients for bone repair, can be prepared at around 0oC, it is expected to be a suitable material for the controlled-release of active ingredients. Sr was reported to promote a cytokine, which inhibited the formation of osteoclasts. Thus, Sr-doped ACP is expected to be a controlled-release material and can promote bone formation. In this study, we synthesized Sr-doped ACP powder by wet synthesis, and prepared Sr-doped calcium phosphate cement. The cement has potential for use as a new type of bone substitute. (Received January 1, 2016; Accepted April 4, 2016)
{"title":"PREPARATION OF Sr-CONTAINING BONE LIKE APATITE CEMENT","authors":"T. Kasai, Tomohiro Uchino","doi":"10.3363/PRB.31.20","DOIUrl":"https://doi.org/10.3363/PRB.31.20","url":null,"abstract":"Amorphous calcium phosphate (ACP) is a known precursor of apatite, which is one of the main components of human bone. Synthesized ACP transforms into low-crystallinity hydroxyapatite (HAp) around body temperature. Thus, if low-crystallinity calcium phosphate cement, containing active ingredients for bone repair, can be prepared at around 0oC, it is expected to be a suitable material for the controlled-release of active ingredients. Sr was reported to promote a cytokine, which inhibited the formation of osteoclasts. Thus, Sr-doped ACP is expected to be a controlled-release material and can promote bone formation. In this study, we synthesized Sr-doped ACP powder by wet synthesis, and prepared Sr-doped calcium phosphate cement. The cement has potential for use as a new type of bone substitute. (Received January 1, 2016; Accepted April 4, 2016)","PeriodicalId":20022,"journal":{"name":"Phosphorus Research Bulletin","volume":"43 1","pages":"20-23"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89473470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Inomata, H. Okano, Shunsuke Watanabe, S. Nakagawa
The evaluation of powder properties and the examination based on ISO 13779-3 and JIS T 0330-3 for calcium phosphates as biomaterial were performed. Powder properties were investigated by X-ray diffractometry (XRD), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), measurement of specific surface area (SSA), particle size analysis and chemical analysis. Crystalline content and chemical impurities were evaluated by the examination based on ISO 13779-3, and the solubility was evaluated by the examination based on JIS T 0330-3. These results indicated that our products were single phase, and crystalline content and chemical impurities were within the range of specification. Moreover, it was found that hydroxyapatite has different characteristics for particle morphology and SSA according to the difference of synthesis methods. It was comfirmed that the elution amounts of calcium (Ca 2+ ) ion per each specific surface area were different among our products from the examination of solubility. Consequently, these products are expected as raw materials for biomaterials, and the requiered products will be supplied by the application of our technology. (Received Oct 25, 2016; Accepted Feb 17, 2016)
根据ISO 13779-3和JIS T 0330-3对作为生物材料的磷酸钙进行粉末性能评价和检验。采用x射线衍射(XRD)、傅里叶变换红外光谱(FT-IR)、扫描电镜(SEM)、比表面积测量(SSA)、粒度分析和化学分析等方法研究了粉末的性能。晶体含量和化学杂质通过基于ISO 13779-3的检查来评估，溶解度通过基于JIS T 0330-3的检查来评估。这些结果表明，我们的产品是单相的，晶体含量和化学杂质在规格范围内。此外，根据合成方法的不同，羟基磷灰石的颗粒形态和SSA具有不同的特征。通过对溶解度的测定，证实了我们的产品在每个比表面积上的钙离子洗脱量是不同的。因此，这些产品有望作为生物材料的原材料，并通过应用我们的技术来提供所需的产品。(2016年10月25日收稿;接受2016年2月17日)
{"title":"EVALUATION OF POWDER PROPERTY OF CALUCIUM PHOSPHATE","authors":"Y. Inomata, H. Okano, Shunsuke Watanabe, S. Nakagawa","doi":"10.3363/PRB.32.17","DOIUrl":"https://doi.org/10.3363/PRB.32.17","url":null,"abstract":"The evaluation of powder properties and the examination based on ISO 13779-3 and JIS T 0330-3 for calcium phosphates as biomaterial were performed. Powder properties were investigated by X-ray diffractometry (XRD), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), measurement of specific surface area (SSA), particle size analysis and chemical analysis. Crystalline content and chemical impurities were evaluated by the examination based on ISO 13779-3, and the solubility was evaluated by the examination based on JIS T 0330-3. These results indicated that our products were single phase, and crystalline content and chemical impurities were within the range of specification. Moreover, it was found that hydroxyapatite has different characteristics for particle morphology and SSA according to the difference of synthesis methods. It was comfirmed that the elution amounts of calcium (Ca 2+ ) ion per each specific surface area were different among our products from the examination of solubility. Consequently, these products are expected as raw materials for biomaterials, and the requiered products will be supplied by the application of our technology. (Received Oct 25, 2016; Accepted Feb 17, 2016)","PeriodicalId":20022,"journal":{"name":"Phosphorus Research Bulletin","volume":"35 1","pages":"17-21"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84770665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kabir, M. Murata, T. Akazawa, K. Yamada, Manabu Ito, T. Shibata
A vital tooth-derived demineralized dentin matrix (DDM) is defined as acid-insoluble, cross-linked collagen with growth factors. The objective of this study is to evaluate the feasibility of the perforated-DDM for bone regeneration. Thirty perforations were added to the human vital wisdom tooth after the removal of enamel portion. The modified tooth was partially demineralized in 0.34 N HNO3 for 30 min and grafted into the critical defect of sheep iliac to evaluate bone regeneration. Sheep were sacrificed for μ-CT and histological evaluation at 2 and 4 months after the graft. New bone was connected directly with the grafted DDM at 2 months. The volume of new bone was comparable with the μ-CT, 3D, and the histological findings at 2 and 4 months. The amount of new bone tissue in the critical defects at 4 months was higher than that at 2 months. The results revealed that the human tooth-derived block material with artificial porous structure, the perforated-DDM, successfully contributed to bone ingrowth as the unique scaffold. This novel human tooth-derived scaffold may be a promising biomaterial to induce bone regeneration in the tri-cortical critical-sized bone defects. (Received January 9, 2016; Accepted March 19, 2016)
{"title":"DEMINERALIZED DENTIN MATRIX WITH ARTIFICIAL PERFORATIONS FOR BONE REGNERATION IN CRITICAL DEFECTS OF ADULT SHEEP","authors":"A. Kabir, M. Murata, T. Akazawa, K. Yamada, Manabu Ito, T. Shibata","doi":"10.3363/PRB.31.1","DOIUrl":"https://doi.org/10.3363/PRB.31.1","url":null,"abstract":"A vital tooth-derived demineralized dentin matrix (DDM) is defined as acid-insoluble, cross-linked collagen with growth factors. The objective of this study is to evaluate the feasibility of the perforated-DDM for bone regeneration. Thirty perforations were added to the human vital wisdom tooth after the removal of enamel portion. The modified tooth was partially demineralized in 0.34 N HNO3 for 30 min and grafted into the critical defect of sheep iliac to evaluate bone regeneration. Sheep were sacrificed for μ-CT and histological evaluation at 2 and 4 months after the graft. New bone was connected directly with the grafted DDM at 2 months. The volume of new bone was comparable with the μ-CT, 3D, and the histological findings at 2 and 4 months. The amount of new bone tissue in the critical defects at 4 months was higher than that at 2 months. The results revealed that the human tooth-derived block material with artificial porous structure, the perforated-DDM, successfully contributed to bone ingrowth as the unique scaffold. This novel human tooth-derived scaffold may be a promising biomaterial to induce bone regeneration in the tri-cortical critical-sized bone defects. (Received January 9, 2016; Accepted March 19, 2016)","PeriodicalId":20022,"journal":{"name":"Phosphorus Research Bulletin","volume":"1 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77209202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SiO2-Li2O-K2O-Al2O3-P2O5 based lithium disilicate glass ceramic was prepared and fabricated into dental crowns. The influence of factors such as pre-crystallization temperature and fabrication technique on the phase formation and morphology of the glass ceramic was investigated. Differential thermal analysis (DTA), X-ray diffraction (XRD), scanning and transmission electron microscopies (SEM and TEM) were performed to determine the thermal properties, the phase formation and microstructure of the samples, respectively. The composition of lithium disilicate glass ceramic could be fabricated into dental crowns through the hot-pressing and CAD/CAM techniques. Similarities in phase formation have been revealed on the dental crown samples after fabrication by both methods. The dental crown samples fabricated by the CAD/CAM technique exhibited significantly finer needle-like crystals of lithium disilicate. The pre-cerammed ingots and fabrication technique have no significant change on the phase formation; however, they play important roles in changing the morphology of the samples in this study. (Received January 01, 2016; Accepted April 30, 2016)
{"title":"EFFECT OF PRE-CRYSTALLIZATION ON LITHIUM DISILICATE GLASS-CERAMICS FABRICATED BY DIFFERENT PROCESSES","authors":"N. Monmaturapoj, A. Srion, Thossapol Chunkiri","doi":"10.3363/PRB.31.24","DOIUrl":"https://doi.org/10.3363/PRB.31.24","url":null,"abstract":"SiO2-Li2O-K2O-Al2O3-P2O5 based lithium disilicate glass ceramic was prepared and fabricated into dental crowns. The influence of factors such as pre-crystallization temperature and fabrication technique on the phase formation and morphology of the glass ceramic was investigated. Differential thermal analysis (DTA), X-ray diffraction (XRD), scanning and transmission electron microscopies (SEM and TEM) were performed to determine the thermal properties, the phase formation and microstructure of the samples, respectively. The composition of lithium disilicate glass ceramic could be fabricated into dental crowns through the hot-pressing and CAD/CAM techniques. Similarities in phase formation have been revealed on the dental crown samples after fabrication by both methods. The dental crown samples fabricated by the CAD/CAM technique exhibited significantly finer needle-like crystals of lithium disilicate. The pre-cerammed ingots and fabrication technique have no significant change on the phase formation; however, they play important roles in changing the morphology of the samples in this study. (Received January 01, 2016; Accepted April 30, 2016)","PeriodicalId":20022,"journal":{"name":"Phosphorus Research Bulletin","volume":"6 1","pages":"24-30"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78358985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Takeshi Yabutsuka, Ryoki Karashima, S. Takai, T. Yao
: We formed many micropores on the surfaces of stainless steel (SUS) substrates by sandblasting method using alumina particles with 14 μm or 3 μm for average particle size and apatite nucleus (AN) treatment was operated. By these treatments, we provided bioactivity to the SUS substrates. We evaluated apatite-forming ability of the SUS substrate by soaking in a simulated body fluid. Apatite formation was induced on the surface of the substrate within 1 day. High adhesive strength of apatite layer was achieved by a mechanical interlocking effect between the apatite layer and the substrate. The adhesive strength was related to the size of the grinding particles in the sandblasting process.
{"title":"EFFECTS OF SANDBLASTING CONDITIONS IN PREPARATION OF BIOACTIVE STAINLESS STEELS BY THE FUNCTION OF APATITE NUCLEI","authors":"Takeshi Yabutsuka, Ryoki Karashima, S. Takai, T. Yao","doi":"10.3363/PRB.31.15","DOIUrl":"https://doi.org/10.3363/PRB.31.15","url":null,"abstract":": We formed many micropores on the surfaces of stainless steel (SUS) substrates by sandblasting method using alumina particles with 14 μm or 3 μm for average particle size and apatite nucleus (AN) treatment was operated. By these treatments, we provided bioactivity to the SUS substrates. We evaluated apatite-forming ability of the SUS substrate by soaking in a simulated body fluid. Apatite formation was induced on the surface of the substrate within 1 day. High adhesive strength of apatite layer was achieved by a mechanical interlocking effect between the apatite layer and the substrate. The adhesive strength was related to the size of the grinding particles in the sandblasting process.","PeriodicalId":20022,"journal":{"name":"Phosphorus Research Bulletin","volume":"27 1","pages":"15-19"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86651275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, we report a simple method for synthesizing HAp particles intended for the selective adsorption of basic proteins. HAp particles for the adsorption of basic proteins were prepared from a mixture of calcium acetate and diammonium hydrogen phosphate solutions, both adjusted at pH 10 (HAp-pH10) and a non-adjusted pH (HAp-none). For HAp-none, the amount of bovine serum albumin (BSA, an acidic protein) adsorbed per unit specific surface area was larger than the amount of lysozyme (LSZ, a basic protein) adsorbed. The ratio of the amounts of protein adsorption, i.e., LSZ/BSA, was 0.32 for HAp-none. The amount of LSZ adsorbed on HAp-pH10 was larger than those for BSA. The ratio of LSZ/BSA was 2.38 on HAp-pH10 aged for 1 h. An increase in the aging time (72 h) caused an increase in LSZ/BSA to 3.17. (Received January 7, 2016; Accepted 2 August, 2016)
{"title":"SYNTHESIS OF HYDROXYAPATITE PARTICLES INTENDED FOR THE SELECTIVE ADSORPTION OF BASIC PROTEINS","authors":"T. Nagasaki, F. Nagata, M. Sakurai, Katsuya Kato","doi":"10.3363/PRB.31.4","DOIUrl":"https://doi.org/10.3363/PRB.31.4","url":null,"abstract":"In this study, we report a simple method for synthesizing HAp particles intended for the selective adsorption of basic proteins. HAp particles for the adsorption of basic proteins were prepared from a mixture of calcium acetate and diammonium hydrogen phosphate solutions, both adjusted at pH 10 (HAp-pH10) and a non-adjusted pH (HAp-none). For HAp-none, the amount of bovine serum albumin (BSA, an acidic protein) adsorbed per unit specific surface area was larger than the amount of lysozyme (LSZ, a basic protein) adsorbed. The ratio of the amounts of protein adsorption, i.e., LSZ/BSA, was 0.32 for HAp-none. The amount of LSZ adsorbed on HAp-pH10 was larger than those for BSA. The ratio of LSZ/BSA was 2.38 on HAp-pH10 aged for 1 h. An increase in the aging time (72 h) caused an increase in LSZ/BSA to 3.17. (Received January 7, 2016; Accepted 2 August, 2016)","PeriodicalId":20022,"journal":{"name":"Phosphorus Research Bulletin","volume":"28 1","pages":"4-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75097773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：