Pub Date : 2023-11-30DOI: 10.1186/s42483-023-00210-z
Can Zhang, Shanshan Chen, Fan Zhang, Yuxin Zheng, Yuke Wang, Xili Liu
{"title":"The La-related protein PsLARP4_5 is crucial for zoospore production and pathogenicity in Phytophthora sojae","authors":"Can Zhang, Shanshan Chen, Fan Zhang, Yuxin Zheng, Yuke Wang, Xili Liu","doi":"10.1186/s42483-023-00210-z","DOIUrl":"https://doi.org/10.1186/s42483-023-00210-z","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":" 27","pages":"1-10"},"PeriodicalIF":3.4,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139206933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-23DOI: 10.1186/s42483-023-00209-6
Yaqin Wang, Tao Hu, Hui Liu, Chenlu Su, Li Xie, Zhanqi Wang, Xueping Zhou
{"title":"Development and application of a self-assembling split-fluorescent protein toolkit to monitor geminiviral movement and infection in plant","authors":"Yaqin Wang, Tao Hu, Hui Liu, Chenlu Su, Li Xie, Zhanqi Wang, Xueping Zhou","doi":"10.1186/s42483-023-00209-6","DOIUrl":"https://doi.org/10.1186/s42483-023-00209-6","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"27 1","pages":"1-10"},"PeriodicalIF":3.4,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139245411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-16DOI: 10.1186/s42483-023-00208-7
Jing Wang, Ziyue Wen, Yun Chen, Zhonghua Ma
{"title":"The sucrose non-fermenting-1 kinase Snf1 is involved in fludioxonil resistance via interacting with the high osmolarity glycerol MAPK kinase Hog1 in Fusarium","authors":"Jing Wang, Ziyue Wen, Yun Chen, Zhonghua Ma","doi":"10.1186/s42483-023-00208-7","DOIUrl":"https://doi.org/10.1186/s42483-023-00208-7","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"1 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139269398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-14DOI: 10.1186/s42483-023-00207-8
M. P. Rabadán, P. Gómez
Abstract Cucurbit aphid-borne yellows virus (CABYV) and watermelon mosaic virus (WMV) are major plant pathogens that cause severe epidemics in cucurbit crops. While there has been an increasing interest in molecular epidemiological studies on both viruses at regional scales, their phylodynamic analysis by using the temporal data at global scale remains unexplored. In this study, we implemented the Nextstrain phylodynamic approach to comprehensively examine the coat protein gene and full-length genome sequences of the CABYV and WMV worldwide populations. Our analyses reconstructed a robust phylogeny of CABYV and confirmed the occurrence of isolates grouped into three clusters (Asian, Mediterranean, and Recombinant). Nextstrain analysis suggested that CABYV epidemics likely originated in Southeast Asia in fourteenth century, while the Mediterranean population emerged in Spain in seventeenth century. We also found a high divergence between Asian and Mediterranean isolates, with low genetic diversity and scarce evidence of selection, as reflected by the lack of gene flow. Moreover, the hierarchical analysis of molecular variance revealed a significant differentiation between CABYV populations grouped by geographical location and plant host. Additionally, the global phylogenetic reconstruction of the WMV population confirmed a clear differentiation among isolates, which grouped into two clusters (classical and emergent), and Nextstrain analysis suggested that WMV epidemics were most probably originated in USA during the sixteenth century. The initial WMV population diverged in the eighteenth century, with the origin of the emergent population in the nineteenth century. Our analysis confirmed that WMV population has a polyphyletic origin, defining an extensive genetic diversity. Overall, this work provides insights into the CABYV and WMV origin and evolutionary dynamics, gaining an understanding of the global spread of both viral diseases. Additionally, the integration of all spatio-temporal CABYV and WMV data, along with their genome sequence data by open access ( https://github.com/PiR92 ) and the Nextstrain approach, provides a valuable tool for compiling and sharing current knowledge of these viral diseases in cucurbit crops.
{"title":"Global phylodynamics of two relevant aphid-transmitted viruses in cucurbit crops: cucurbit aphid-borne yellows virus and watermelon mosaic virus","authors":"M. P. Rabadán, P. Gómez","doi":"10.1186/s42483-023-00207-8","DOIUrl":"https://doi.org/10.1186/s42483-023-00207-8","url":null,"abstract":"Abstract Cucurbit aphid-borne yellows virus (CABYV) and watermelon mosaic virus (WMV) are major plant pathogens that cause severe epidemics in cucurbit crops. While there has been an increasing interest in molecular epidemiological studies on both viruses at regional scales, their phylodynamic analysis by using the temporal data at global scale remains unexplored. In this study, we implemented the Nextstrain phylodynamic approach to comprehensively examine the coat protein gene and full-length genome sequences of the CABYV and WMV worldwide populations. Our analyses reconstructed a robust phylogeny of CABYV and confirmed the occurrence of isolates grouped into three clusters (Asian, Mediterranean, and Recombinant). Nextstrain analysis suggested that CABYV epidemics likely originated in Southeast Asia in fourteenth century, while the Mediterranean population emerged in Spain in seventeenth century. We also found a high divergence between Asian and Mediterranean isolates, with low genetic diversity and scarce evidence of selection, as reflected by the lack of gene flow. Moreover, the hierarchical analysis of molecular variance revealed a significant differentiation between CABYV populations grouped by geographical location and plant host. Additionally, the global phylogenetic reconstruction of the WMV population confirmed a clear differentiation among isolates, which grouped into two clusters (classical and emergent), and Nextstrain analysis suggested that WMV epidemics were most probably originated in USA during the sixteenth century. The initial WMV population diverged in the eighteenth century, with the origin of the emergent population in the nineteenth century. Our analysis confirmed that WMV population has a polyphyletic origin, defining an extensive genetic diversity. Overall, this work provides insights into the CABYV and WMV origin and evolutionary dynamics, gaining an understanding of the global spread of both viral diseases. Additionally, the integration of all spatio-temporal CABYV and WMV data, along with their genome sequence data by open access ( https://github.com/PiR92 ) and the Nextstrain approach, provides a valuable tool for compiling and sharing current knowledge of these viral diseases in cucurbit crops.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"81 13","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134900999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-07DOI: 10.1186/s42483-023-00206-9
Ya-Zhou Zhang, Zhuo Li, Jie Man, Dan Xu, Lan Wen, Chen Yang, Qiang Xu, Qian-Tao Jiang, Guo-Yue Chen, Mei Deng, Yun-Liang Peng, Kai Zhong, Peng-Fei Qi, Yu-Ming Wei
Abstract Fusarium head blight (FHB) caused by Fusarium species, seriously threatens the safety of wheat ( Triticum aestivum ) production. Resistant cultivars and fungicides are frequently used to control these FHB pathogens. However, Fusarium species have been adapting the current FHB control approaches in a manner that raises concern for future FHB control strategies, which could lead to a greater risk of FHB outbreaks. In this study, a total of 521 strains of Fusarium were isolated from Sichuan province of China, to investigate the diversity of Fusarium species and the genes associated with their adaptation. Seven species were identified based on molecular markers and morphological analysis. The virulence assays showed that Fusarium asiaticum (Fa) and Fusarium graminearum (Fg) were the two major causal agents of FHB, with high virulence and more frequent isolates. Fungicide resistance analysis showed that four isolates had developed the resistance to carbendazim, and four isolates had developed the resistance to tebuconazole. Of note, two point-mutation variants (F200Y and E198Q) occurred in the β2-tubulin gene, leading to the carbendazim resistance. The landscape of genomic diversity was analyzed through whole-genome sequencing, revealing a total of 182,811 and 430,733 variants (including: single nucleotide polymorphisms, SNP, insertion and deletion, Indel, and structure variation, SV) among the Fa and Fg isolates, respectively. In addition, potential alterations in gene function (15.22%) were predicted among Fg variants. These alterations offer potential helps for the Fusarium species to adapt to various managements of FHB, which may increase risks in developing fungicide-resistant isolates. However, these annotated genetic variants are valuable resources for further genetic and genomic studies, as well as potential markers to assist disease risk assessment.
摘要赤霉病(Fusarium head blight, FHB)是由镰刀菌引起的一种疫病，严重威胁小麦(Triticum aestivum)的生产安全。抗性品种和杀菌剂经常用于控制这些FHB病原体。然而，镰刀菌一直在适应目前的食物毒素控制方法，这引起了人们对未来食物毒素控制策略的关注，这可能导致更大的食物毒素暴发风险。本研究从中国四川省分离了521株镰刀菌，研究了镰刀菌的物种多样性及其适应相关基因。通过分子标记和形态分析鉴定出7种。毒力测定结果表明，亚洲镰刀菌(Fa)和谷物镰刀菌(Fg)是FHB的两种主要病原，毒力高，分离频率高。4株菌株对多菌灵产生耐药性，4株菌株对戊康唑产生耐药性。值得注意的是，β2-微管蛋白基因发生了两个点突变变异(F200Y和E198Q)，导致多菌灵耐药。通过全基因组测序分析了菌株Fa和Fg的基因组多样性格局，分别发现了182,811和430,733个变异(包括:单核苷酸多态性、SNP、插入和缺失、Indel和结构变异SV)。此外，在Fg变异中预测基因功能的潜在改变(15.22%)。这些变化为镰刀菌种适应FHB的各种管理提供了潜在的帮助，这可能增加开发抗杀菌剂分离株的风险。然而，这些注释的遗传变异是进一步遗传和基因组研究的宝贵资源，也是协助疾病风险评估的潜在标记。
{"title":"Genetic diversity of field Fusarium asiaticum and Fusarium graminearum isolates increases the risk of fungicide resistance","authors":"Ya-Zhou Zhang, Zhuo Li, Jie Man, Dan Xu, Lan Wen, Chen Yang, Qiang Xu, Qian-Tao Jiang, Guo-Yue Chen, Mei Deng, Yun-Liang Peng, Kai Zhong, Peng-Fei Qi, Yu-Ming Wei","doi":"10.1186/s42483-023-00206-9","DOIUrl":"https://doi.org/10.1186/s42483-023-00206-9","url":null,"abstract":"Abstract Fusarium head blight (FHB) caused by Fusarium species, seriously threatens the safety of wheat ( Triticum aestivum ) production. Resistant cultivars and fungicides are frequently used to control these FHB pathogens. However, Fusarium species have been adapting the current FHB control approaches in a manner that raises concern for future FHB control strategies, which could lead to a greater risk of FHB outbreaks. In this study, a total of 521 strains of Fusarium were isolated from Sichuan province of China, to investigate the diversity of Fusarium species and the genes associated with their adaptation. Seven species were identified based on molecular markers and morphological analysis. The virulence assays showed that Fusarium asiaticum (Fa) and Fusarium graminearum (Fg) were the two major causal agents of FHB, with high virulence and more frequent isolates. Fungicide resistance analysis showed that four isolates had developed the resistance to carbendazim, and four isolates had developed the resistance to tebuconazole. Of note, two point-mutation variants (F200Y and E198Q) occurred in the β2-tubulin gene, leading to the carbendazim resistance. The landscape of genomic diversity was analyzed through whole-genome sequencing, revealing a total of 182,811 and 430,733 variants (including: single nucleotide polymorphisms, SNP, insertion and deletion, Indel, and structure variation, SV) among the Fa and Fg isolates, respectively. In addition, potential alterations in gene function (15.22%) were predicted among Fg variants. These alterations offer potential helps for the Fusarium species to adapt to various managements of FHB, which may increase risks in developing fungicide-resistant isolates. However, these annotated genetic variants are valuable resources for further genetic and genomic studies, as well as potential markers to assist disease risk assessment.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"49 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135432492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-10DOI: 10.1186/s42483-023-00203-y
Zhejun Huang, Zhenxi Ji, Juan Wang, Zhanpeng Li, Zhoumian Jiang, Wei Ni, Hongyan Chen, Taiyun Wei, Xiao-Feng Zhang
Abstract Rice yellow stunt virus (RYSV) P3 protein functions as a movement protein during viral infection of a plant host; its function in insect hosts remains unclear. In this study, we investigated the subcellular localization of P3 using leafhopper ( Nephotettix cincticeps ) cell cultures. Our results showed that P3 translocated from the cytoplasm to the nucleus in RYSV-infected leafhopper cells, where it interacted with the viral N protein as a constituent of viroplasms. Interfering with the P3 gene expression significantly suppressed viral infection in N. cincticeps . Finally, we demonstrate that the nuclear translocation of P3 in leafhopper cells depended on its interaction with RYSV N protein, which enters the nucleus via an interaction with importin α3. These findings unveil a previously unknown role for P3 in RYSV infection of the insect vector and provide valuable insights into the infection dynamics of plant rhabdoviruses.
{"title":"Rice yellow stunt virus p3 protein enters the nucleus of leafhopper cell and localizes to viroplasm through interaction with N protein via importin α3-mediated pathway","authors":"Zhejun Huang, Zhenxi Ji, Juan Wang, Zhanpeng Li, Zhoumian Jiang, Wei Ni, Hongyan Chen, Taiyun Wei, Xiao-Feng Zhang","doi":"10.1186/s42483-023-00203-y","DOIUrl":"https://doi.org/10.1186/s42483-023-00203-y","url":null,"abstract":"Abstract Rice yellow stunt virus (RYSV) P3 protein functions as a movement protein during viral infection of a plant host; its function in insect hosts remains unclear. In this study, we investigated the subcellular localization of P3 using leafhopper ( Nephotettix cincticeps ) cell cultures. Our results showed that P3 translocated from the cytoplasm to the nucleus in RYSV-infected leafhopper cells, where it interacted with the viral N protein as a constituent of viroplasms. Interfering with the P3 gene expression significantly suppressed viral infection in N. cincticeps . Finally, we demonstrate that the nuclear translocation of P3 in leafhopper cells depended on its interaction with RYSV N protein, which enters the nucleus via an interaction with importin α3. These findings unveil a previously unknown role for P3 in RYSV infection of the insect vector and provide valuable insights into the infection dynamics of plant rhabdoviruses.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136291821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-06DOI: 10.1186/s42483-023-00205-w
Aihong Zhou, Yaoxuan Feng, Xinpei Gao, Yue Liu, Fan Ji, Lili Huang, Zhensheng Kang, Gangming Zhan
Abstract Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is a devastating disease that seriously threatens the production of crops worldwide. Triadimefon is the widely-used fungicide for controlling the disease in China; however, as the fungicide targets a single site (position 401 in the 134th codon of the Cyp51 gene), the extensive application imposes a strong selection pressure on the pathogens, which may potentially lose the effect over time. In this study, 176 Pst field isolates sampled from different regions of Xinjiang were determined for their sensitivity to triadimefon because it is the few frequent Pst outbreak and representative area in China. The results showed that the Pst isolates collected from Yili, Xinjiang, exhibited a strong resistance to triadimefon with an average EC 50 of 0.263 µg/mL, despite the rest of the isolates maintaining high sensitivity to triadimefon. The triadimefon-resistant and triadimefon-sensitive isolates did not display significant differences in sporulation, but the triadimefon-resistant isolates exhibited weaker adaptive traits in their latent period and urediniospore germination rate than the triadimefon-sensitive isolates. No cross-resistance was found for the other two fungicides, flubeneteram or pyraclostrobin; however, cross-resistance for the demethylation inhibitor (DMI) fungicides, tebuconazole and hexaconazole, was found. Genome sequencing revealed that the Tyrosine (Y) at 134 residue was mutated to Phenylalanine (F) in the Xinjiang isolates. Our study revealed that a natural mutation in Pst led to the efficacy loss of triadimefon to control the disease.
{"title":"Characterization of the triadimefon resistant Puccinia striiformis f. sp. tritici isolates in China","authors":"Aihong Zhou, Yaoxuan Feng, Xinpei Gao, Yue Liu, Fan Ji, Lili Huang, Zhensheng Kang, Gangming Zhan","doi":"10.1186/s42483-023-00205-w","DOIUrl":"https://doi.org/10.1186/s42483-023-00205-w","url":null,"abstract":"Abstract Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is a devastating disease that seriously threatens the production of crops worldwide. Triadimefon is the widely-used fungicide for controlling the disease in China; however, as the fungicide targets a single site (position 401 in the 134th codon of the Cyp51 gene), the extensive application imposes a strong selection pressure on the pathogens, which may potentially lose the effect over time. In this study, 176 Pst field isolates sampled from different regions of Xinjiang were determined for their sensitivity to triadimefon because it is the few frequent Pst outbreak and representative area in China. The results showed that the Pst isolates collected from Yili, Xinjiang, exhibited a strong resistance to triadimefon with an average EC 50 of 0.263 µg/mL, despite the rest of the isolates maintaining high sensitivity to triadimefon. The triadimefon-resistant and triadimefon-sensitive isolates did not display significant differences in sporulation, but the triadimefon-resistant isolates exhibited weaker adaptive traits in their latent period and urediniospore germination rate than the triadimefon-sensitive isolates. No cross-resistance was found for the other two fungicides, flubeneteram or pyraclostrobin; however, cross-resistance for the demethylation inhibitor (DMI) fungicides, tebuconazole and hexaconazole, was found. Genome sequencing revealed that the Tyrosine (Y) at 134 residue was mutated to Phenylalanine (F) in the Xinjiang isolates. Our study revealed that a natural mutation in Pst led to the efficacy loss of triadimefon to control the disease.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135303303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Natural rhizosphere bacteria has the potential to act as an alternative of chemical pesticides for sustainable agriculture. In the current study, tobacco rhizosphere Bacillus velezensis D exhibited great antibacterial effect against Ralstonia solanacearum , and significantly enhanced the tobacco resistance against bacterial wilt in pot experiments. Then Bacillus velezensis D was labeled with gfp marker and found to stably colonize in tobacco root, the colonization density of strain D in root still remained 5.33 × 10 4 CFU/gat 30 days post-inoculation. Subsequently, field trials for two years (2021–2022) showed the control effects of the strain D on the tobacco bacterial wilt were 12.26% and 36.37%, respectively, indicating the application of B. velezensis D could improve plant resistance to R.solanacearum . In order to further study the antibacterial activities of strain D, effects of the crude extracts on the swimming ability, cell viability and the morphology of R. solanacearum were analyzed. The results showed that the crude extracts reduced the motility of R. solanacearum , and caused cell wall rupture and cell death. Furthermore, MALDI-TOF-MS and HPLC-QTOF-MS analysis indicated that lipopeptides (fengycin and iturin) and polyketides (bacillaene) were detected in the crude extracts of strain D. Based on these findings, we speculated that Bacillus velezensis D firstly colonized in tobacco root, then produced antibacterial substances at ecological sites to exert antagonistic effects, inhibiting motility traits of R. solanacearum and damaging the cell well. Hence, Bacillus velezensis D could be used as a potential biological control agents against tobacco bacterial wilt.
{"title":"Biocontrol and molecular characterization of Bacillus velezensis D against tobacco bacterial wilt","authors":"Jiawei Wang, Yulong Peng, Shanshan Xie, Xinru Yu, Chongyu Bian, Huiping Wu, Yun Wang, Ting Ding","doi":"10.1186/s42483-023-00204-x","DOIUrl":"https://doi.org/10.1186/s42483-023-00204-x","url":null,"abstract":"Abstract Natural rhizosphere bacteria has the potential to act as an alternative of chemical pesticides for sustainable agriculture. In the current study, tobacco rhizosphere Bacillus velezensis D exhibited great antibacterial effect against Ralstonia solanacearum , and significantly enhanced the tobacco resistance against bacterial wilt in pot experiments. Then Bacillus velezensis D was labeled with gfp marker and found to stably colonize in tobacco root, the colonization density of strain D in root still remained 5.33 × 10 4 CFU/gat 30 days post-inoculation. Subsequently, field trials for two years (2021–2022) showed the control effects of the strain D on the tobacco bacterial wilt were 12.26% and 36.37%, respectively, indicating the application of B. velezensis D could improve plant resistance to R.solanacearum . In order to further study the antibacterial activities of strain D, effects of the crude extracts on the swimming ability, cell viability and the morphology of R. solanacearum were analyzed. The results showed that the crude extracts reduced the motility of R. solanacearum , and caused cell wall rupture and cell death. Furthermore, MALDI-TOF-MS and HPLC-QTOF-MS analysis indicated that lipopeptides (fengycin and iturin) and polyketides (bacillaene) were detected in the crude extracts of strain D. Based on these findings, we speculated that Bacillus velezensis D firstly colonized in tobacco root, then produced antibacterial substances at ecological sites to exert antagonistic effects, inhibiting motility traits of R. solanacearum and damaging the cell well. Hence, Bacillus velezensis D could be used as a potential biological control agents against tobacco bacterial wilt.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135695702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Dickeya dadantii is the causal agent of bacterial stalk rot and one of the most destructive and widespread diseases of the sorghum in the world. Here, we explored microbe-based approaches for managing this destructive pathogen, intending to provide alternatives for integrated disease management. The objective of the research was to decipher the effect of antagonistic microbes on systemic defense enzymes, histochemical changes, plant growth attributes, reduction in disease severity, and interaction of these antagonistic microbes with host. Trichoderma , Pseudomonas , and Bacillus isolates were collected from rhizospheric soil and characterized using morphological and molecular tools. ITS and 16S rRNA sequences were analyzed to determine the molecular characterization of all antagonist microbes, and they were identified as T. asperellum, T. viride , T. harzianum, B. subtilis , and P. flourescens . These isolates were evaluated for antibacterial properties against D. dadantii under in vitro conditions and showed the higher inhibition in a dual culture method. Further, the effects of seed bio-priming and soil application of these isolates were tested under glasshouse and field conditions. T. viride outperformed the other isolates, significantly enhancing the plant growth parameters and induced resistance to Dickeya dadantii (BSR). T. viride showed a significantly higher accumulation of defensive enzymes, viz. PAL (1.02), PO (1.70), PPO (1.25), CAT (1.11), and TPC (0.91) at 48 h after pathogen challenge, as compared to the control. Histochemical tests confirmed lignification and callose deposition in the cell walls of the treated plants. Antagonist microbes were further evaluated under field conditions against D. dadantii infection. Compared to the control, there is a significant enhancement of plant growth parameters and yield with a simultaneous decrease in disease severity in T. viride treated plants. Results showed that the potential benefits of T. viride could not only effectively induce resistance in plants, enhance plant growth, increase yield, and suppress pathogen infection but also reduce the use of hazardous pesticides. As a result of correlation, PCA and heat map analyses indicated that T. viride is interconnected to determine the crop ability to sustain its growth under pathogen stress.
{"title":"Elicitation of native bio protective microbial agents associated systemic defense responses and plant growth promotion against bacterial stalk rot pathogen in sorghum (Sorghum bicolor)","authors":"Sujata Singh Yadav, Anshul Arya, Vishal Singh, Yogendra Singh","doi":"10.1186/s42483-023-00202-z","DOIUrl":"https://doi.org/10.1186/s42483-023-00202-z","url":null,"abstract":"Abstract Dickeya dadantii is the causal agent of bacterial stalk rot and one of the most destructive and widespread diseases of the sorghum in the world. Here, we explored microbe-based approaches for managing this destructive pathogen, intending to provide alternatives for integrated disease management. The objective of the research was to decipher the effect of antagonistic microbes on systemic defense enzymes, histochemical changes, plant growth attributes, reduction in disease severity, and interaction of these antagonistic microbes with host. Trichoderma , Pseudomonas , and Bacillus isolates were collected from rhizospheric soil and characterized using morphological and molecular tools. ITS and 16S rRNA sequences were analyzed to determine the molecular characterization of all antagonist microbes, and they were identified as T. asperellum, T. viride , T. harzianum, B. subtilis , and P. flourescens . These isolates were evaluated for antibacterial properties against D. dadantii under in vitro conditions and showed the higher inhibition in a dual culture method. Further, the effects of seed bio-priming and soil application of these isolates were tested under glasshouse and field conditions. T. viride outperformed the other isolates, significantly enhancing the plant growth parameters and induced resistance to Dickeya dadantii (BSR). T. viride showed a significantly higher accumulation of defensive enzymes, viz. PAL (1.02), PO (1.70), PPO (1.25), CAT (1.11), and TPC (0.91) at 48 h after pathogen challenge, as compared to the control. Histochemical tests confirmed lignification and callose deposition in the cell walls of the treated plants. Antagonist microbes were further evaluated under field conditions against D. dadantii infection. Compared to the control, there is a significant enhancement of plant growth parameters and yield with a simultaneous decrease in disease severity in T. viride treated plants. Results showed that the potential benefits of T. viride could not only effectively induce resistance in plants, enhance plant growth, increase yield, and suppress pathogen infection but also reduce the use of hazardous pesticides. As a result of correlation, PCA and heat map analyses indicated that T. viride is interconnected to determine the crop ability to sustain its growth under pathogen stress.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136235748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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