Pub Date : 2023-09-08DOI: 10.1080/00318884.2023.2235235
Li-Chia Liu, Showe-Mei Lin, Phaik-Eem Lim
ABSTRACT The non-geniculate coralline algal genus Crustaphytum occurs in subtropical regions of the Pacific and Atlantic Oceans and presently contains only two species, C. pacificum and C. atlanticum. In contrast, the genus Mesophyllum is more widely reported and has representative species in both warm and cold waters. We analysed the species diversity of collections of Crustaphytum and Mesophyllum-like specimens from the Asian-Pacific region, based on psbA and rbcL sequence analyses and morphology. Our molecular analyses showed that the collections of Mesophyllum-like specimens mostly belonged to Crustaphytum with only a few specimens clustering with the Mesophyllum/Melyvonnea complex. We also describe three new species of Crustaphytum (C. taiwani-boreale sp. nov., C. taoyuanense sp. nov. and C. fragile sp. nov.) that are commonly found in the algal reefs in Northwest Taiwan, utilizing both scanning electron and light microscopy, and DNA sequence analyses. Crustaphytum fragile (79–95 μm thick) and Crustaphytum taiwani-boreale (235–436 μm thick) mostly grow in similar habitats in the algal reefs and can only be distinguished by a combination of characters relating to tetrasporangial chamber size and the number of cell layers in the cortex and medulla. Crustaphytum taoyuanense (233–300 μm thick) can be separated from the other species by its violet-red colour and by the margins of older thalli becoming overgrown by adjacent thalli. Crustaphytum taoyuanense and C. fragile are restricted to Taoyuan City in Northwestern Taiwan, whereas C. taiwani-boreale has a broader distribution in Northern Taiwan. Our study reveals a rich species diversity of Crustaphytum in the warm water region of the Western Pacific Ocean.
{"title":"Species diversity and molecular phylogeny of Crustaphytum and Mesophyllum (Hapalidiales, Rhodophyta) from the Asian Pacific, including three new species of Crustaphytum","authors":"Li-Chia Liu, Showe-Mei Lin, Phaik-Eem Lim","doi":"10.1080/00318884.2023.2235235","DOIUrl":"https://doi.org/10.1080/00318884.2023.2235235","url":null,"abstract":"ABSTRACT The non-geniculate coralline algal genus Crustaphytum occurs in subtropical regions of the Pacific and Atlantic Oceans and presently contains only two species, C. pacificum and C. atlanticum. In contrast, the genus Mesophyllum is more widely reported and has representative species in both warm and cold waters. We analysed the species diversity of collections of Crustaphytum and Mesophyllum-like specimens from the Asian-Pacific region, based on psbA and rbcL sequence analyses and morphology. Our molecular analyses showed that the collections of Mesophyllum-like specimens mostly belonged to Crustaphytum with only a few specimens clustering with the Mesophyllum/Melyvonnea complex. We also describe three new species of Crustaphytum (C. taiwani-boreale sp. nov., C. taoyuanense sp. nov. and C. fragile sp. nov.) that are commonly found in the algal reefs in Northwest Taiwan, utilizing both scanning electron and light microscopy, and DNA sequence analyses. Crustaphytum fragile (79–95 μm thick) and Crustaphytum taiwani-boreale (235–436 μm thick) mostly grow in similar habitats in the algal reefs and can only be distinguished by a combination of characters relating to tetrasporangial chamber size and the number of cell layers in the cortex and medulla. Crustaphytum taoyuanense (233–300 μm thick) can be separated from the other species by its violet-red colour and by the margins of older thalli becoming overgrown by adjacent thalli. Crustaphytum taoyuanense and C. fragile are restricted to Taoyuan City in Northwestern Taiwan, whereas C. taiwani-boreale has a broader distribution in Northern Taiwan. Our study reveals a rich species diversity of Crustaphytum in the warm water region of the Western Pacific Ocean.","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47377771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACTA new marine woloszynskioid dinoflagellate, Biecheleria donggangensis sp. nov., germinated from the sediments of Rizhao coastal area, China, was examined by light and scanning electron microscopy. Its phylogenetic position was inferred from nuclear rDNA (partial LSU, SSU and ITS1–5.8S–ITS2) sequences. Cells were spherical to ellipsoidal (8.5‒19.2 μm long and 5.9‒11.5 μm wide) with numerous small and discoid chloroplasts, and a centrally located nucleus. Scanning electron microscopy showed an apical furrow area consisting of a single elongated apical vesicle (EAV) with 20 to 34 small globular knobs at the top of the epicone, and 13–15 latitudinal series of amphiesmal vesicles (AVs). Biecheleria donggangensis can be distinguished from other congeneric species by the configuration of the AVs bordering the EAV, the number of latitudinal AVs, and the trichocyst pore (TP) structures around the EAV. Thick-walled and spherical (or oval) cysts were 16.1‒23.5 μm in diameter and decorated with needle-like processes, each with a distal swelling. Molecular phylogenies using maximum likelihood (ML) were examined by three statistical methods (bootstrap, approximate Bayes and the Shimodaira-Hasegawa-like approximate likelihood-ratio test), which clearly demonstrated that the isolate belonged to the genus Biecheleria but formed a clade distinct from all previously described species. Genetic distance analyses justified describing the isolate as a new species rather than a new ribotype of B. cincta.KEYWORDS: Amphiesmal vesicle (AV)DinoflagellatesGenetic distanceNucleus-encoded rDNAPhylogeny DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary InformationSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2273655Additional informationFundingThis work was financially supported by the National Science Foundation of China (No. 42106199), the Science & Technology Basic Resources Investigation Program of China (2018FY100204) and the Science and Technology Innovation Project of Laoshan Laboratory (LSKJ202203700).
{"title":"<i>Biecheleria donggangensis sp. nov</i> . (Suessiaceae, Dinophyceae), a new marine woloszynskioid species germinated from coastal sediment of the Yellow Sea, China","authors":"Yuyang Liu, Zhangxi Hu, Haifeng Gu, Zhe Tao, Yunyan Deng, Lixia Shang, Zhaoyang Chai, Ying Zhong Tang","doi":"10.1080/00318884.2023.2273655","DOIUrl":"https://doi.org/10.1080/00318884.2023.2273655","url":null,"abstract":"ABSTRACTA new marine woloszynskioid dinoflagellate, Biecheleria donggangensis sp. nov., germinated from the sediments of Rizhao coastal area, China, was examined by light and scanning electron microscopy. Its phylogenetic position was inferred from nuclear rDNA (partial LSU, SSU and ITS1–5.8S–ITS2) sequences. Cells were spherical to ellipsoidal (8.5‒19.2 μm long and 5.9‒11.5 μm wide) with numerous small and discoid chloroplasts, and a centrally located nucleus. Scanning electron microscopy showed an apical furrow area consisting of a single elongated apical vesicle (EAV) with 20 to 34 small globular knobs at the top of the epicone, and 13–15 latitudinal series of amphiesmal vesicles (AVs). Biecheleria donggangensis can be distinguished from other congeneric species by the configuration of the AVs bordering the EAV, the number of latitudinal AVs, and the trichocyst pore (TP) structures around the EAV. Thick-walled and spherical (or oval) cysts were 16.1‒23.5 μm in diameter and decorated with needle-like processes, each with a distal swelling. Molecular phylogenies using maximum likelihood (ML) were examined by three statistical methods (bootstrap, approximate Bayes and the Shimodaira-Hasegawa-like approximate likelihood-ratio test), which clearly demonstrated that the isolate belonged to the genus Biecheleria but formed a clade distinct from all previously described species. Genetic distance analyses justified describing the isolate as a new species rather than a new ribotype of B. cincta.KEYWORDS: Amphiesmal vesicle (AV)DinoflagellatesGenetic distanceNucleus-encoded rDNAPhylogeny DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary InformationSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2273655Additional informationFundingThis work was financially supported by the National Science Foundation of China (No. 42106199), the Science & Technology Basic Resources Investigation Program of China (2018FY100204) and the Science and Technology Innovation Project of Laoshan Laboratory (LSKJ202203700).","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134948895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-03DOI: 10.1080/00318884.2023.2241123
Luanda Pereira Soares, Silvia M.P.B. Guimarães, Mutue Toyota Fujii
ABSTRACTMolecular sequence analyses of specimens previously identified as Cirrulicarpus sp. in the southeastern Brazil (Espírito Santo coast), indicated that they are more closely related to the genus Meredithia than to the genus Cirrulicarpus. The new species Meredithia dichotoma is herein proposed to accommodate this uncommon and endemic taxon, morphologically characterized by an erect, narrow, strap-shaped thallus, up to 16 cm long, mostly dichotomously branched, with regular constrictions, densely to moderately filamentous medulla, with occasional ganglionic cells, and an expanded medullary region in the basal transverse sections, resembling a midrib in surface view. The species is also supported by the sequence divergence from its congeners: ≥4.3% and 2.2% in the COI-5P and rbcL markers, respectively. Meredithia dichotoma differs from other 11 species of the genus by its dimensions and general habit, never forming expanded blades. This is a new genus record for Brazil and the first species of Meredithia known to occur in the western South Atlantic, increasing the known diversity in the red algal family Kallymeniaceae.KEYWORDS: COI-5PGigartinalesMolecular phylogenyrbcLRhodoliths bedsTaxonomy ACKNOWLEDGEMENTSWe thank Feresa Cabrera for providing sequences of Meredithia hawaiiensis, and Gary Saunders by sequences of Commanderella, Hommersandia and Velatocarpus. LPS thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Bolsista CAPES/BRASIL 88887.515974/2020-00) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for Postdoctoral Fellowship (152282/2022-0). MTF thanks CNPq for the Productivity Fellowship (311195/2021-0).Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2241123DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Additional informationFundingThis study was partially supported by a research grant from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2016/50370-7 and 2017/50341-0).
摘要对巴西东南部(Espírito Santo coast)的Cirrulicarpus sp.标本的分子序列分析表明，它们与Meredithia属的亲缘关系比Cirrulicarpus属的亲缘关系更近。本文提出的新物种Meredithia dichotoma是为了适应这种罕见的特有分类群，其形态学特征是直立，狭窄，带状状的菌体，长可达16厘米，大部分为二分分支，具有规则的收缩，密集至中等丝状的髓质，偶有神经节细胞，在基底横切面有一个扩大的髓质区域，表面上看类似中脉。COI-5P和rbcL标记的序列差异分别≥4.3%和2.2%，也支持了该物种。由它的尺寸和一般习性，从没有形成展开的叶片不同于其他11种的属。这是巴西的新属记录，也是已知出现在南大西洋西部的Meredithia的第一个物种，增加了红藻科Kallymeniaceae的已知多样性。关键词:coi - 5pgigartinales;分子系统发育学;rhodoliths床分类感谢Feresa Cabrera提供了Meredithia hawaiiensis的序列，Gary Saunders提供了Commanderella、Hommersandia和Velatocarpus的序列。LPS感谢Nível Superior (Bolsista CAPES/BRASIL 88887.515974/2020-00)和Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)的博士后资助(152282/2022-0)。MTF感谢CNPq提供的生产力奖学金(311195/2021-0)。补充材料本文的补充数据可在https://doi.org/10.1080/00318884.2023.2241123DISCLOSURE网站上获取。statement作者未报告潜在的利益冲突。本研究的部分资金由圣保罗州安帕罗基金(FAPESP 2016/50370-7和2017/50341-0)的研究经费支持。
{"title":"Molecular assessment of specimens previously assigned to <i>Cirrulicarpus</i> (Kallymeniaceae, Rhodophyta) in Brazil, with the description of <i>Meredithia dichotoma sp. nov.</i>","authors":"Luanda Pereira Soares, Silvia M.P.B. Guimarães, Mutue Toyota Fujii","doi":"10.1080/00318884.2023.2241123","DOIUrl":"https://doi.org/10.1080/00318884.2023.2241123","url":null,"abstract":"ABSTRACTMolecular sequence analyses of specimens previously identified as Cirrulicarpus sp. in the southeastern Brazil (Espírito Santo coast), indicated that they are more closely related to the genus Meredithia than to the genus Cirrulicarpus. The new species Meredithia dichotoma is herein proposed to accommodate this uncommon and endemic taxon, morphologically characterized by an erect, narrow, strap-shaped thallus, up to 16 cm long, mostly dichotomously branched, with regular constrictions, densely to moderately filamentous medulla, with occasional ganglionic cells, and an expanded medullary region in the basal transverse sections, resembling a midrib in surface view. The species is also supported by the sequence divergence from its congeners: ≥4.3% and 2.2% in the COI-5P and rbcL markers, respectively. Meredithia dichotoma differs from other 11 species of the genus by its dimensions and general habit, never forming expanded blades. This is a new genus record for Brazil and the first species of Meredithia known to occur in the western South Atlantic, increasing the known diversity in the red algal family Kallymeniaceae.KEYWORDS: COI-5PGigartinalesMolecular phylogenyrbcLRhodoliths bedsTaxonomy ACKNOWLEDGEMENTSWe thank Feresa Cabrera for providing sequences of Meredithia hawaiiensis, and Gary Saunders by sequences of Commanderella, Hommersandia and Velatocarpus. LPS thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Bolsista CAPES/BRASIL 88887.515974/2020-00) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for Postdoctoral Fellowship (152282/2022-0). MTF thanks CNPq for the Productivity Fellowship (311195/2021-0).Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2241123DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Additional informationFundingThis study was partially supported by a research grant from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2016/50370-7 and 2017/50341-0).","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-03DOI: 10.1080/00318884.2023.2268381
Balasubramanian Karthick, Murugesan Yogeshwaran, J. Patrick Kociolek
ABSTRACTWe present light and SEM observations of a new astigmate gomphonemoid diatom from the Eastern Ghats of India. This species, and a closely allied one from the Western Ghats that has a stigma, are similar to other members of the Gomphonemataceae in terms of symmetry and certain valve features. They differ, however, in having groups of differentiated pore fields at both apices. At the headpole, the area bearing the groups of pores is flattened and positioned on the valve mantle, and is not easily seen in light microscopy. For these species we propose the genus Indiconema gen. nov. within the Gomphonemataceae. We evaluate the phylogenetic position of this group and relationships within the family based on valve and girdle band features, which suggest that the new genus is sister to Afrocymbella, a genus apparently endemic to Africa. We compare this basal phylogenetic position with other groups of organisms also found in the Western and Eastern Ghats of India, as well as to fossil taxa in the Gomphonemataceae. The sister taxon relationship with a genus known only from Africa supports a close relationship of the floras of the two regions.KEYWORDS: BiraphidDiatom phylogenyGomphonemataceaeHerculeana groupWestern Ghats ACKNOWLEDGEMENTSB. Karthick thanks the Director of Agharkar Research Institute for facilities and encouragement and Dr. Jonathan Taylor, North-West University, Potchefstroom, South Africa.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Additional informationFundingThis research was supported by the Science and Engineering Research Board (SERB, CRG/2021/004668), India. The field expedition was supported by a National Geographic Society Exploration Grant (Grant Number: 9224-12).
摘要:本文报道了一种来自印度东高止山脉的新的无尖藻形硅藻的光和扫描电镜观察。这个物种，和一个来自西高特山脉的有柱头的紧密联系的物种，在对称和某些阀特征方面与Gomphonemataceae的其他成员相似。然而，它们的不同之处在于在两端都有不同的孔场群。在顶杆处，承载气孔群的区域被压平并位于阀套上，在光学显微镜下不容易看到。对于这些种，我们建议将其归为贡花科的Indiconema gen. 11。我们根据瓣带和带带的特征评估了这一类群的系统发育位置和科内的关系，这表明该新属是非洲特有属非洲伞菌的姐妹属。我们将这一基础系统发育位置与在印度西高止山脉和东高止山脉发现的其他生物群以及Gomphonemataceae化石分类群进行了比较。与非洲已知的一个属的姊妹分类群关系支持了这两个地区的植物区系的密切关系。关键词:双足硅藻系统发育gomphonemataceae herculleana群西高止山脉Karthick感谢Agharkar研究所所长提供的设施和鼓励以及南非Potchefstroom西北大学的Jonathan Taylor博士。声明作者未报告潜在的利益冲突。本研究得到了印度科学与工程研究委员会(塞尔维亚，CRG/2021/004668)的支持。这次野外考察是由国家地理学会探险基金(资助号:9224-12)资助的。
{"title":"A new freshwater gomphonemoid diatom genus from India, with the description of a new species from the Eastern Ghats","authors":"Balasubramanian Karthick, Murugesan Yogeshwaran, J. Patrick Kociolek","doi":"10.1080/00318884.2023.2268381","DOIUrl":"https://doi.org/10.1080/00318884.2023.2268381","url":null,"abstract":"ABSTRACTWe present light and SEM observations of a new astigmate gomphonemoid diatom from the Eastern Ghats of India. This species, and a closely allied one from the Western Ghats that has a stigma, are similar to other members of the Gomphonemataceae in terms of symmetry and certain valve features. They differ, however, in having groups of differentiated pore fields at both apices. At the headpole, the area bearing the groups of pores is flattened and positioned on the valve mantle, and is not easily seen in light microscopy. For these species we propose the genus Indiconema gen. nov. within the Gomphonemataceae. We evaluate the phylogenetic position of this group and relationships within the family based on valve and girdle band features, which suggest that the new genus is sister to Afrocymbella, a genus apparently endemic to Africa. We compare this basal phylogenetic position with other groups of organisms also found in the Western and Eastern Ghats of India, as well as to fossil taxa in the Gomphonemataceae. The sister taxon relationship with a genus known only from Africa supports a close relationship of the floras of the two regions.KEYWORDS: BiraphidDiatom phylogenyGomphonemataceaeHerculeana groupWestern Ghats ACKNOWLEDGEMENTSB. Karthick thanks the Director of Agharkar Research Institute for facilities and encouragement and Dr. Jonathan Taylor, North-West University, Potchefstroom, South Africa.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Additional informationFundingThis research was supported by the Science and Engineering Research Board (SERB, CRG/2021/004668), India. The field expedition was supported by a National Geographic Society Exploration Grant (Grant Number: 9224-12).","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-03DOI: 10.1080/00318884.2023.2259243
Rúben Luz, Rita Cordeiro, Jan Kaštovský, Jeffrey R. Johansen, Elisabete Dias, Amélia Fonseca, Ralph Urbatzka, Vitor Vasconcelos, Vítor Gonçalves
ABSTRACTThe taxonomy of cyanobacteria has advanced quickly with the use of molecular methods in combination with well-defined morphological and ecological characters. Using this approach, many taxonomical changes have occurred in the Synechococcales and Oscillatoriales, with the description of new families, genera and species. Here we present the study of four cyanobacterial strains from the Bank of Algae and Cyanobacteria of the Azores (BACA) culture collection, all isolated from the Azores Archipelago. The strains were genetically characterized through the amplification of the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer (ITS) region, as well as morphologically by light and transmission electron microscopy. One of the strains presented a high genetic divergence from known genera of Synechococcales and was described as Venetifunis florensis gen. & sp. nov. (Trichocoleusaceae, Cyanobacteria). The remaining three strains were found to be new species of the genera Albertania, Kovacikia and Pegethrix. Venetifunis florensis was isolated from the shores of Lake Rasa in Flores Island, Kovacikia atmophytica sp. nov. is a new species mainly distinguished genetically from other Kovacikia species from Terceira Island. Albertania obscura sp. nov. was isolated from inside a volcanic cave in Terceira Island and is distinct both morphologically and genetically from the already described species. Pegethrix atlantica sp. nov. is a new morphologically and genetically different species isolated from São Miguel Island. The description of these new taxa increases the biogeographic knowledge of the four mentioned cyanobacterial genera to remote oceanic islands and new terrestrial habitats.KEYWORDS: AerophyticAtlantic islandsAtmophyticVenetifunis minimusLeptolyngbyaceaeOculatellaceaeTrichocoleusaceae ACKNOWLEDGEMENTSWe thank the association ‘Os Montanheiros’ for the access to Algar do Carvão.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2259243Additional informationFundingRúben Luz was supported by a Ph.D. grant (M3.1.a/F/002/2020) from the Fundo Regional da Ciência e Tecnologia (FRCT). This work was funded by FEDER funds through the Interreg-MAC 2014-2020 Program under the projects ‘REBECA – Red de excelencia en biotecnología azul (algas) de la región macaronesia (MAC1.1a/060)’ and ‘REBECA-CCT – Red de Excelencia en Biotecnología Azul de la Región Macaronésica. Consolidación, Certificación y Transferencia (MAC2/1.1b/269)’, and by Portuguese National Funds through Foundation for Science and Technology (FCT), under the project UIDB/50027/2020. CIIMAR acknowledges funding by FCT through UIDB/04423/2020 and UIDP/04423/2020. JK and JRJ were supported by Project Inter Excellence LTAUSA 18008.
摘要蓝藻的分子分类学在结合清晰的形态学和生态学特征的基础上取得了迅速的进展。利用这种方法，聚球菌科和振球菌科的分类学发生了许多变化，出现了新的科、属和种。在这里，我们提出了四种蓝藻菌株的研究从藻类银行和亚速尔蓝藻(BACA)培养收集，所有从亚速尔群岛分离。通过16S rRNA基因和16S- 23s rRNA内部转录间隔区(ITS)的扩增，以及光镜和透射电镜对菌株进行了遗传表征。其中一个菌株与已知的聚球菌属存在高度遗传差异，被描述为Venetifunis florensis gen. & sp. 11 . (Trichocoleusaceae，蓝藻门)。其余3株分别为Albertania属、Kovacikia属和Pegethrix属新种。科瓦齐亚(Kovacikia atmophytica sp. 11 .)是一种分离自弗洛雷斯岛Rasa湖岸边的新种，主要从遗传上区别于其他科瓦齐亚种。Albertania obscura sp. 11 .是从Terceira岛的一个火山洞穴中分离出来的，在形态和基因上都与已经描述的物种不同。洋蓟(Pegethrix atlantica sp. 11 .)是在米格尔岛分离到的一个形态和遗传上不同的新种。这些新分类群的描述增加了上述四个蓝藻属的生物地理知识到遥远的海洋岛屿和新的陆地栖息地。关键词:空气植物学;大西洋岛屿;植物植物学;声明作者未报告潜在的利益冲突。本文的补充数据可在https://doi.org/10.1080/00318884.2023.2259243Additional informationFundingRúben上在线获取，Luz得到了Fundo Regional da Ciência e Tecnologia (FRCT)的博士资助(M3.1.a/F/002/2020)。本研究由FEDER基金通过interrega - mac 2014-2020计划资助，项目分为“REBECA- Red de excellence en biotecnología azul (algas) de la región macaronacacia (MAC1.1a/060)”和“REBECA- cct - Red de excellence en Biotecnología azul de la Región macaronacacia”。Consolidación, Certificación y Transferencia (MAC2/1.1b/269) '，并由葡萄牙国家基金通过科学和技术基金会(FCT)在UIDB/50027/2020项目下提供。CIIMAR通过UIDB/04423/2020和UIDP/04423/2020确认FCT资助。JK和JRJ得到了LTAUSA 18008卓越项目的支持。
{"title":"New terrestrial cyanobacteria from the Azores Islands: description of <i>Venetifunis gen. nov</i> . and new species of <i>Albertania, Kovacikia</i> and <i>Pegethrix</i>","authors":"Rúben Luz, Rita Cordeiro, Jan Kaštovský, Jeffrey R. Johansen, Elisabete Dias, Amélia Fonseca, Ralph Urbatzka, Vitor Vasconcelos, Vítor Gonçalves","doi":"10.1080/00318884.2023.2259243","DOIUrl":"https://doi.org/10.1080/00318884.2023.2259243","url":null,"abstract":"ABSTRACTThe taxonomy of cyanobacteria has advanced quickly with the use of molecular methods in combination with well-defined morphological and ecological characters. Using this approach, many taxonomical changes have occurred in the Synechococcales and Oscillatoriales, with the description of new families, genera and species. Here we present the study of four cyanobacterial strains from the Bank of Algae and Cyanobacteria of the Azores (BACA) culture collection, all isolated from the Azores Archipelago. The strains were genetically characterized through the amplification of the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer (ITS) region, as well as morphologically by light and transmission electron microscopy. One of the strains presented a high genetic divergence from known genera of Synechococcales and was described as Venetifunis florensis gen. & sp. nov. (Trichocoleusaceae, Cyanobacteria). The remaining three strains were found to be new species of the genera Albertania, Kovacikia and Pegethrix. Venetifunis florensis was isolated from the shores of Lake Rasa in Flores Island, Kovacikia atmophytica sp. nov. is a new species mainly distinguished genetically from other Kovacikia species from Terceira Island. Albertania obscura sp. nov. was isolated from inside a volcanic cave in Terceira Island and is distinct both morphologically and genetically from the already described species. Pegethrix atlantica sp. nov. is a new morphologically and genetically different species isolated from São Miguel Island. The description of these new taxa increases the biogeographic knowledge of the four mentioned cyanobacterial genera to remote oceanic islands and new terrestrial habitats.KEYWORDS: AerophyticAtlantic islandsAtmophyticVenetifunis minimusLeptolyngbyaceaeOculatellaceaeTrichocoleusaceae ACKNOWLEDGEMENTSWe thank the association ‘Os Montanheiros’ for the access to Algar do Carvão.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2259243Additional informationFundingRúben Luz was supported by a Ph.D. grant (M3.1.a/F/002/2020) from the Fundo Regional da Ciência e Tecnologia (FRCT). This work was funded by FEDER funds through the Interreg-MAC 2014-2020 Program under the projects ‘REBECA – Red de excelencia en biotecnología azul (algas) de la región macaronesia (MAC1.1a/060)’ and ‘REBECA-CCT – Red de Excelencia en Biotecnología Azul de la Región Macaronésica. Consolidación, Certificación y Transferencia (MAC2/1.1b/269)’, and by Portuguese National Funds through Foundation for Science and Technology (FCT), under the project UIDB/50027/2020. CIIMAR acknowledges funding by FCT through UIDB/04423/2020 and UIDP/04423/2020. JK and JRJ were supported by Project Inter Excellence LTAUSA 18008.","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-03DOI: 10.1080/00318884.2023.2271359
Albert Reñé
"Marine benthic dinoflagellates – their relevance for science and society (2nd, completely revised edition)." Phycologia, ahead-of-print(ahead-of-print), p. 1
海洋底栖甲藻-它们与科学和社会的相关性(第2版，完全修订版)。《生理学》，印前版，第1页
{"title":"Marine benthic dinoflagellates – their relevance for science and society (2nd, completely revised edition) <b>Marine benthic dinoflagellates – their relevance for science and society (2nd, completely revised edition)</b> . Mona Hoppenrath, Nicolas Chomérat, Takeo Horiguchi, Shauna A. Murray & Lesley Rhodes2023. 376 pp. Senckenberg Gesellschaft für Naturforschung, Frankfurt am Main, Germany. [Senckenberg Buch vol. 88.] 34.90€ [hardback: ISBN 9783510614240].","authors":"Albert Reñé","doi":"10.1080/00318884.2023.2271359","DOIUrl":"https://doi.org/10.1080/00318884.2023.2271359","url":null,"abstract":"\"Marine benthic dinoflagellates – their relevance for science and society (2nd, completely revised edition).\" Phycologia, ahead-of-print(ahead-of-print), p. 1","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-03DOI: 10.1080/00318884.2023.2254621
Narongrit Muangmai, Sinchai Maneekat, Anong Chirapart, Giuseppe C. Zuccarello
ABSTRACTFor long-term management and conservation of marine resources an understanding of their genetic diversity and geographic distribution is necessary. The red alga Gracilaria salicornia is widely distributed along the coasts of Thailand and has been used as food and as fertilizer. However, there have been no studies examining the genetic diversity of this alga. The present research aimed to employ two different genetic markers, mitochondrial (COI-5P) and chloroplast (RuBisCO spacer) for exploring the genetic diversity and phylogeography of G. salicornia in Thailand. Our analyses, inferred from both markers, revealed different levels of genetic diversity between the two coasts of Thailand, and the recognition of seven new COI haplotypes. Gracilaria salicornia in the Andaman Sea had higher genetic diversity than in the Gulf of Thailand. Significant genetic divergence was found between populations in the Andaman Sea and the Gulf of Thailand, likely due to the geographic barrier of the Thai-Malay Peninsula and the complex ocean currents. No genetic structuring was detected in the Gulf of Thailand, whereas low to moderate genetic differentiation was found between populations in the Andaman Sea, suggesting a possible break between the northern and southern Andaman Sea. This break could be explained by ocean circulation patterns around the Malacca Strait. These findings provide further evidence supporting the Thai-Malay Peninsula and oceanographic currents as strong barriers to dispersal, leading to genetic differentiation and population structuring of G. salicornia and other marine species. These results can be used to guide conservation management actions and exploitation of this marine resource.KEYWORDS: COIMacroalgaePopulation geneticsPhylogeographyRhodophyta ACKNOWLEDGEMENTSWe thank Jantana Praiboon and Jakaphan Bulan for help collecting and sequencing samples. We also thank the associate editor Gavin Maneveldt and two anonymous reviewers for their comments and suggestions.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary InformationSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2254621Additional informationFundingThis work was supported by a research grant to NM from the Thailand Research Fund (MRG6080040).
{"title":"Contrasting patterns of genetic diversity and population discontinuity in the common red seaweed <i>Gracilaria salicornia</i> (Gracilariaceae) along the coasts of Thailand","authors":"Narongrit Muangmai, Sinchai Maneekat, Anong Chirapart, Giuseppe C. Zuccarello","doi":"10.1080/00318884.2023.2254621","DOIUrl":"https://doi.org/10.1080/00318884.2023.2254621","url":null,"abstract":"ABSTRACTFor long-term management and conservation of marine resources an understanding of their genetic diversity and geographic distribution is necessary. The red alga Gracilaria salicornia is widely distributed along the coasts of Thailand and has been used as food and as fertilizer. However, there have been no studies examining the genetic diversity of this alga. The present research aimed to employ two different genetic markers, mitochondrial (COI-5P) and chloroplast (RuBisCO spacer) for exploring the genetic diversity and phylogeography of G. salicornia in Thailand. Our analyses, inferred from both markers, revealed different levels of genetic diversity between the two coasts of Thailand, and the recognition of seven new COI haplotypes. Gracilaria salicornia in the Andaman Sea had higher genetic diversity than in the Gulf of Thailand. Significant genetic divergence was found between populations in the Andaman Sea and the Gulf of Thailand, likely due to the geographic barrier of the Thai-Malay Peninsula and the complex ocean currents. No genetic structuring was detected in the Gulf of Thailand, whereas low to moderate genetic differentiation was found between populations in the Andaman Sea, suggesting a possible break between the northern and southern Andaman Sea. This break could be explained by ocean circulation patterns around the Malacca Strait. These findings provide further evidence supporting the Thai-Malay Peninsula and oceanographic currents as strong barriers to dispersal, leading to genetic differentiation and population structuring of G. salicornia and other marine species. These results can be used to guide conservation management actions and exploitation of this marine resource.KEYWORDS: COIMacroalgaePopulation geneticsPhylogeographyRhodophyta ACKNOWLEDGEMENTSWe thank Jantana Praiboon and Jakaphan Bulan for help collecting and sequencing samples. We also thank the associate editor Gavin Maneveldt and two anonymous reviewers for their comments and suggestions.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary InformationSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2254621Additional informationFundingThis work was supported by a research grant to NM from the Thailand Research Fund (MRG6080040).","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-03DOI: 10.1080/00318884.2023.2244813
Armando Mendoza-Flores, M. del Pilar Sánchez-Saavedra
ABSTRACTAmphidinium carterae is a dinoflagellate of increasing interest for producing bioactive compounds. The aim of this study was to investigate the effect of five irradiances (50, 100, 150, 200 and 250 µmol photon m–2 s–1) on the fatty acid profile of A. carterae. The results show that high irradiances increase the lipid productivity (36.65 mg l–1 d–1 at 50 µmol photon m–2 s–1 to 90 mg l–1 d–1 at 250 µmol photon m–2 s–1). High irradiance increased the content of polyunsaturated fatty acids (PUFAs) relative to the total content of fatty acids (40.0% at 50 µmol photon m–2 s–1 to 53.23% at 250 µmol photon m–2 s–1) and decreased the amount of monounsaturated fatty acids (MUFAs) (14.30% at 50 µmol photon m–2 s–1 to 6.04% at 250 µmol photon m–2 s–1). The higher PUFAs content was due to docosahexaenoic acid (DHA; 20.76% at 50 µmol photon m–2 s–1 to 30.63% at 250 µmol photon m–2 s–1) and eicosapentaenoic acid (EPA; 14.91% at 50 µmol photon m–2 s–1 to 18.96% at 250 µmol photon m–2 s–1). Palmitic acid (C16:0) had the highest content among saturated fatty acids (19.87% at 50 µmol photon m–2 s–1 to 22.29% at 250 µmol photon m–2 s–1). The proportion of neutral lipids tended to increase under high irradiances (100–250 µmol photon m–2 s–1). At the lowest irradiance (50 µmol photon m–2 s–1), the proportion of polar lipids was higher than that of neutral lipids. PUFAs had the main content within polar lipids at the five irradiances (47%–50%). The major neutral lipids were SFAs at the five irradiances (47%–51%). It was concluded that the irradiance levels influence the lipid metabolism of A. carterae. The increase in irradiance level raises the production of lipids and the content of EPA and DHA for potential utilization in the biotechnological, aquaculture and nutraceutical industries.KEYWORDS: DinoflagellatesDocosahexaenoic acidLipid classesPolyunsaturated fatty acids AUTHOR CONTRIBUTIONSA.M.F.: design and implementation of the experiments and manuscript preparation.M.P.S.S. supervision of experiments and data processing, contribution to manuscript preparation, and financial support.STATEMENT OF INFORMED CONSENT, HUMAN/ANIMAL RIGHTSNo conflicts, informed consent, or human or animal rights are applicable to this study.ACKNOWLEDGEMENTSThis work has been funded by Consejo Nacional de Ciencia y Tecnología (CONACyT) (Grant Project: SEP-CONACyT 130074), and CICESE (Grant Project: 623108). A.M.F. thanks the postdoctoral scholarship from the Oceanography Division of CICESE and the postdoctoral scholarship from CONACyT.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Additional informationFundingThis work was supported by the Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California [623108].
摘要黄斑双鞭毛藻是一种因生产生物活性化合物而日益受到关注的鞭毛藻。研究了50、100、150、200和250µmol光子m-2 s-1辐照对黄芪脂肪酸谱的影响。结果表明，高辐照度提高了脂质产量(50µmol光子m-2 s-1时为36.65 mg l-1 d-1, 250µmol光子m-2 s-1时为90 mg l-1 d-1)。高辐照增加了多不饱和脂肪酸(PUFAs)相对于脂肪酸总含量的含量(在50µmol光子m-2 s-1下为40.0%，在250µmol光子m-2 s-1下为53.23%)，降低了单不饱和脂肪酸(MUFAs)的含量(在50µmol光子m-2 s-1下为14.30%，在250µmol光子m-2 s-1下为6.04%)。PUFAs含量较高是由于二十二碳六烯酸(DHA);50µmol光子m-2 s-1时为20.76%，250µmol光子m-2 s-1时为30.63%)和二十碳五烯酸(EPA;50µmol光子m-2 s-1时为14.91%，250µmol光子m-2 s-1时为18.96%)。饱和脂肪酸中棕榈酸(C16:0)的含量最高，在50µmol光子m-2 s-1时为19.87%，在250µmol光子m-2 s-1时为22.29%。高辐照度(100-250µmol光子m-2 s-1)下，中性脂的比例有增加的趋势。在最低辐照度(50µmol光子m-2 s-1)下，极性脂质比例高于中性脂质比例。5种辐照度下PUFAs主要含量在极性脂质内(47% ~ 50%)。在5种辐照度(47%-51%)下，主要中性脂质为sfa。综上所述，光照水平影响了黄颡鱼的脂质代谢。辐照水平的增加提高了脂类的产量以及EPA和DHA的含量，可用于生物技术、水产养殖和营养保健工业。关键词:甲藻;二十二碳六烯酸;脂类;多不饱和脂肪酸;监督实验和数据处理，参与稿件准备，并提供资金支持。知情同意、人类/动物权利声明本研究不涉及冲突、知情同意或人类/动物权利。本工作由国家科学委员会Tecnología (CONACyT)(资助项目:SEP-CONACyT 130074)和CICESE(资助项目:623108)资助。A.M.F.感谢中国科学院海洋学部博士后奖学金和中国科学院海洋研究所博士后奖学金。声明作者未报告潜在的利益冲突。本研究得到了Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California的支持[623108]。
{"title":"Light irradiance modifies the fatty acid composition of <i>Amphidinium carterae</i> (Dinophyceae)","authors":"Armando Mendoza-Flores, M. del Pilar Sánchez-Saavedra","doi":"10.1080/00318884.2023.2244813","DOIUrl":"https://doi.org/10.1080/00318884.2023.2244813","url":null,"abstract":"ABSTRACTAmphidinium carterae is a dinoflagellate of increasing interest for producing bioactive compounds. The aim of this study was to investigate the effect of five irradiances (50, 100, 150, 200 and 250 µmol photon m–2 s–1) on the fatty acid profile of A. carterae. The results show that high irradiances increase the lipid productivity (36.65 mg l–1 d–1 at 50 µmol photon m–2 s–1 to 90 mg l–1 d–1 at 250 µmol photon m–2 s–1). High irradiance increased the content of polyunsaturated fatty acids (PUFAs) relative to the total content of fatty acids (40.0% at 50 µmol photon m–2 s–1 to 53.23% at 250 µmol photon m–2 s–1) and decreased the amount of monounsaturated fatty acids (MUFAs) (14.30% at 50 µmol photon m–2 s–1 to 6.04% at 250 µmol photon m–2 s–1). The higher PUFAs content was due to docosahexaenoic acid (DHA; 20.76% at 50 µmol photon m–2 s–1 to 30.63% at 250 µmol photon m–2 s–1) and eicosapentaenoic acid (EPA; 14.91% at 50 µmol photon m–2 s–1 to 18.96% at 250 µmol photon m–2 s–1). Palmitic acid (C16:0) had the highest content among saturated fatty acids (19.87% at 50 µmol photon m–2 s–1 to 22.29% at 250 µmol photon m–2 s–1). The proportion of neutral lipids tended to increase under high irradiances (100–250 µmol photon m–2 s–1). At the lowest irradiance (50 µmol photon m–2 s–1), the proportion of polar lipids was higher than that of neutral lipids. PUFAs had the main content within polar lipids at the five irradiances (47%–50%). The major neutral lipids were SFAs at the five irradiances (47%–51%). It was concluded that the irradiance levels influence the lipid metabolism of A. carterae. The increase in irradiance level raises the production of lipids and the content of EPA and DHA for potential utilization in the biotechnological, aquaculture and nutraceutical industries.KEYWORDS: DinoflagellatesDocosahexaenoic acidLipid classesPolyunsaturated fatty acids AUTHOR CONTRIBUTIONSA.M.F.: design and implementation of the experiments and manuscript preparation.M.P.S.S. supervision of experiments and data processing, contribution to manuscript preparation, and financial support.STATEMENT OF INFORMED CONSENT, HUMAN/ANIMAL RIGHTSNo conflicts, informed consent, or human or animal rights are applicable to this study.ACKNOWLEDGEMENTSThis work has been funded by Consejo Nacional de Ciencia y Tecnología (CONACyT) (Grant Project: SEP-CONACyT 130074), and CICESE (Grant Project: 623108). A.M.F. thanks the postdoctoral scholarship from the Oceanography Division of CICESE and the postdoctoral scholarship from CONACyT.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Additional informationFundingThis work was supported by the Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California [623108].","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-03DOI: 10.1080/00318884.2023.2247713
Antony Otinga Oteng’o, Boo Yeon Won, Tae Oh Cho
ABSTRACTEndoplura gyeokpoensis sp. nov. and E. limpeticola sp. nov. from Korea are described based on molecular and morphological analyses. The crustose brown algal genus Endoplura previously comprised three species: E. aurea, E. jejuensis and E. koreana. Our phylogenetic analyses inferred from rbcL and concatenated rbcL and COI-5P data revealed that E. gyeokpoensis sp. nov. and E. limpeticola sp. nov. are placed in the Endoplura clade with robust bootstrap support and Bayesian posterior probabilities. Endoplura gyeokpoensis is characterized by small crustose thalli, thallus outline with a lighter margin, and 2–4 plurangia terminated by 2–3 sterile cells. Endoplura limpeticola is distinguished by epizoic crustose thalli on limpets, thick thalli up to 751 μm, thallus outline with a darker margin, multicellular rhizoids, and two plurangia terminated by 2–3 sterile cells. Our study shows that the two new Endoplura species are molecularly and morphologically distinguished from each other as well as from the congeners.KEYWORDS: COI-5PEpizoicMorphologyPhylogenyrbcLTaxonomy ACKNOWLEDGEMENTSWe are grateful to Dr. Shinya Uwai for his valuable comments on the article and to two anonymous reviewers for improving the manuscript.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary InformationSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2247713Additional informationFundingThis study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A2059577), by the Ministry of Ocean and Fisheries (Marine Biotics Project, 20210469), and by the National Marine Biodiversity Institute of Korea (the management of Marine Fishery Bio-resources Center 2023) to Tae Oh Cho. This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A1A01051909) to Boo Yeon Won.
{"title":"<i>Endoplura gyeokpoensis sp. nov</i> . and <i>E. limpeticola sp. nov</i> . (Ralfsiales, Phaeophyceae), two new crustose brown algae from Korea","authors":"Antony Otinga Oteng’o, Boo Yeon Won, Tae Oh Cho","doi":"10.1080/00318884.2023.2247713","DOIUrl":"https://doi.org/10.1080/00318884.2023.2247713","url":null,"abstract":"ABSTRACTEndoplura gyeokpoensis sp. nov. and E. limpeticola sp. nov. from Korea are described based on molecular and morphological analyses. The crustose brown algal genus Endoplura previously comprised three species: E. aurea, E. jejuensis and E. koreana. Our phylogenetic analyses inferred from rbcL and concatenated rbcL and COI-5P data revealed that E. gyeokpoensis sp. nov. and E. limpeticola sp. nov. are placed in the Endoplura clade with robust bootstrap support and Bayesian posterior probabilities. Endoplura gyeokpoensis is characterized by small crustose thalli, thallus outline with a lighter margin, and 2–4 plurangia terminated by 2–3 sterile cells. Endoplura limpeticola is distinguished by epizoic crustose thalli on limpets, thick thalli up to 751 μm, thallus outline with a darker margin, multicellular rhizoids, and two plurangia terminated by 2–3 sterile cells. Our study shows that the two new Endoplura species are molecularly and morphologically distinguished from each other as well as from the congeners.KEYWORDS: COI-5PEpizoicMorphologyPhylogenyrbcLTaxonomy ACKNOWLEDGEMENTSWe are grateful to Dr. Shinya Uwai for his valuable comments on the article and to two anonymous reviewers for improving the manuscript.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary InformationSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2247713Additional informationFundingThis study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A2059577), by the Ministry of Ocean and Fisheries (Marine Biotics Project, 20210469), and by the National Marine Biodiversity Institute of Korea (the management of Marine Fishery Bio-resources Center 2023) to Tae Oh Cho. This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A1A01051909) to Boo Yeon Won.","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-03DOI: 10.1080/00318884.2023.2247175
Xuan-Vy Nguyen, Lawrence M. Liao, Showe-Mei Lin
ABSTRACTGrateloupia sensu lato has had a long and troublesome taxonomic history because of superficial similarities in morphology, ill-defined species boundaries and lack of diagnostic characters that leave many gaps that still need to be fully resolved. In this study, we carried out detailed observations on female reproductive structures, and inferred the phylogenetic relationships of the Grateloupia-like species occurring in Vietnam based on rbcL and LSU rDNA sequence analyses. The new species Phyllymenia nhatrangensis sp. nov. differs from its sister species P. gibbesii in the thickness of the cortex. The new species is slightly flattened, composed of two to four main blades, bearing numerous marginal and a few superficial, lanceolate bladelets. Blade sizes of P. nhatrangensis are also smaller than those of P. gibbesii, which are longer and wider. However, the new species shares with species from western Pacific Ocean, such as P. taiwanensis and P. huangiae, the presence of frequent marginal proliferations. Based on rbcL, the divergence between P. nhatrangensis and P. gibbesii was 3.3% (39 bp). With the present addition, Grateloupia sensu lato now comprises 16 species, including nine species of Grateloupia, three species of Phyllymenia, two species of Yonagunia and one species each of Dermocorynus and Prionitis.KEYWORDS: Auxiliary cell ampullaeMorphologyPhylogenetic analysis ACKNOWLEDGEMENTSWe thank Dr. Dao Viet Ha, director of the Institute of Oceanography, the staff of its Department of Marine Botany, and the Vietnam Academy of Science and Technology Key Lab on Food and Environmental Safety (Central Vietnam), Institute of Oceanography, Vietnam for the permission to use their equipment. LML acknowledges the travel support granted by the Graduate School of Integrated Sciences for Life, Hiroshima University. The critical suggestions from two anonymous reviewers have helped improve the manuscript and are gratefully acknowledged.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary InformationSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2247175Additional informationFundingThis work was supported by Vietnam Academy of Science and Technology, under Grant TĐĐTB0.04/21-23.
【摘要】由于在形态上的表面相似性、种界不清以及缺乏诊断特征等方面存在许多有待全面解决的空白，使得拉托格鲁皮亚(grateloupia sensu lato)的分类历史漫长而麻烦。在本研究中，我们通过对雌性生殖结构的详细观察，并基于rbcL和LSU rDNA序列分析推断了越南发生的Grateloupia-like物种的系统发育关系。新种Phyllymenia nhatrangensis sp. 11 .与其姊妹种P. gibbesii的皮层厚度不同。新种稍扁平，由二到四个主叶片组成，有许多边缘和一些表面，披针形的小片。叶面尺寸也比长臂猿小，长而宽。然而，新种与来自西太平洋的p.t aiensis和p.p huangiae共享，存在频繁的边缘增殖。基于rbcL, P. nhatrangensis与P. gibbesii的差异为3.3% (39 bp)。加上目前的增加，现在有16种，其中包括9种Grateloupia, 3种Phyllymenia, 2种Yonagunia和1种Dermocorynus和Prionitis。感谢越南海洋研究所所长Dao Viet Ha博士、海洋植物系工作人员和越南科学院食品与环境安全(越南中部)重点实验室允许我们使用他们的设备。LML感谢广岛大学生命综合科学研究生院提供的旅行支持。两位匿名审稿人提出的重要建议有助于改进本文，我们对此表示感谢。声明作者未报告潜在的利益冲突。本文的补充数据可在https://doi.org/10.1080/00318884.2023.2247175Additional上获得。本研究由越南科学技术研究院资助，资助项目TĐĐTB0.04/21-23。
{"title":"Systematic revision of the <i>Grateloupia</i> complex (Rhodophyta) from Vietnam using <i>rbc</i> L and LSU sequences, with the description of <i>Phyllymenia nhatrangensis sp. nov.</i>","authors":"Xuan-Vy Nguyen, Lawrence M. Liao, Showe-Mei Lin","doi":"10.1080/00318884.2023.2247175","DOIUrl":"https://doi.org/10.1080/00318884.2023.2247175","url":null,"abstract":"ABSTRACTGrateloupia sensu lato has had a long and troublesome taxonomic history because of superficial similarities in morphology, ill-defined species boundaries and lack of diagnostic characters that leave many gaps that still need to be fully resolved. In this study, we carried out detailed observations on female reproductive structures, and inferred the phylogenetic relationships of the Grateloupia-like species occurring in Vietnam based on rbcL and LSU rDNA sequence analyses. The new species Phyllymenia nhatrangensis sp. nov. differs from its sister species P. gibbesii in the thickness of the cortex. The new species is slightly flattened, composed of two to four main blades, bearing numerous marginal and a few superficial, lanceolate bladelets. Blade sizes of P. nhatrangensis are also smaller than those of P. gibbesii, which are longer and wider. However, the new species shares with species from western Pacific Ocean, such as P. taiwanensis and P. huangiae, the presence of frequent marginal proliferations. Based on rbcL, the divergence between P. nhatrangensis and P. gibbesii was 3.3% (39 bp). With the present addition, Grateloupia sensu lato now comprises 16 species, including nine species of Grateloupia, three species of Phyllymenia, two species of Yonagunia and one species each of Dermocorynus and Prionitis.KEYWORDS: Auxiliary cell ampullaeMorphologyPhylogenetic analysis ACKNOWLEDGEMENTSWe thank Dr. Dao Viet Ha, director of the Institute of Oceanography, the staff of its Department of Marine Botany, and the Vietnam Academy of Science and Technology Key Lab on Food and Environmental Safety (Central Vietnam), Institute of Oceanography, Vietnam for the permission to use their equipment. LML acknowledges the travel support granted by the Graduate School of Integrated Sciences for Life, Hiroshima University. The critical suggestions from two anonymous reviewers have helped improve the manuscript and are gratefully acknowledged.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary InformationSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2247175Additional informationFundingThis work was supported by Vietnam Academy of Science and Technology, under Grant TĐĐTB0.04/21-23.","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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