Physiology and Molecular Biology of Plants最新文献

Nanoparticles, thanks to their superior properties such as large surface area and high reactivity, can be an alternative to traditional fertilizers for improving nutrient uptake. Furthermore, considering that chemical and physical synthesis methods require high energy consumption and cause environmental pollution, plant-mediated green synthesis of NPs has attracted great attention since it provides eco-friendly, biocompatible, and inexpensive solutions. In this present study, plant mediated green synthesis of Iron Oxide (Fe₂O₃), Zinc Oxide (ZnO) and Titanium Dioxide (TiO₂) nanoparticles by using Laurus nobilis leaves (bay leaves) were carried out and their structural properties were characterized by UV visible spectra, Dynamic Light Scattering (DLS), Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD) and Transmission Electron Microscopy (TEM). UV spectrum and FTIR analysis exhibited characteristic peaks indicating the presence of the desired NPs, while DLS analysis and TEM images confirmed that synthesized particles are in nano-scale. The potential of nanoparticles as biofertilizer in agricultural uses were assessed by investigating their effects on sunflower growth in hydroponic system. TEM images of the NP applied plant tissues proved the uptake and translocation of NPs from root to leaf. Furthermore, Fe₂O₃, ZnO and TiO₂ NP applications on sunflower up to 5 ppm generally improved physiological growth parameters such as root length, fresh weight and leaf surface area while 20 ppm of Fe₂O₃ and ZnO NPs application cause a significant decrease.

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Bryophyllum pinnatum (Lam.) Oken, a multipurpose medicinal herb, has drawn much interest for its therapeutic qualities from both traditional and modern medicine systems. Many active secondary metabolites, such as bufadienolides, triterpenes, phenols, alkaloids, glycosides, lipids, flavonoids, and organic acids, are responsible for the plant's curative properties. B. pinnatum exhibits a noteworthy significance in oncological research by exhibiting its ability to modify numerous pathways, which may suggest a potential anticancer impact. The herb is recommended for treating lithiasis, a common cause of renal failure, due to its effectiveness in dissolving stones and avoiding crystal formation. The plant has a major impact on diabetes, especially type II diabetes. Moreover, the versatility of B. pinnatum extends to its examination in connection to COVID-19. However, caution is warranted, as B. pinnatum has been reported to possess toxicity attributed to the presence of bufadienolides in its metabolic profile. A comprehensive investigation is essential to thoroughly understand and confirm the synthesis of potentially hazardous compounds. This is crucial for minimizing their presence and ensuring the safe consumption of B. pinnatum among diverse populations of organisms. This review highlights the various medical uses of B. pinnatum, including its ability to effectively treat kidney and liver diseases, as well as its anti-leishmanial, neuropharmacological, antibacterial, immunosuppressive, anti-tumour, and cytotoxic effects. While extensively employed in both traditional and scientific domains, the plant's complete medicinal potential, molecular mechanisms, safety profile, and pharmacodynamics remain ambiguous, rendering it an ideal candidate for pioneering research endeavours.

Melon (Cucumis melo L.) is a horticultural crop that is planted globally. Cucumis melo L. cv. Baogua is a typical melon that is suitable for studying fruit development because of its ability to adapt to different climatic conditions. Long non-coding RNAs (lncRNAs) are a class of non-coding RNAs longer than 200 nucleotides, which play important roles in a wide range of biological processes by regulating gene expression. In this study, the transcriptome of the Baogua melon was sequenced at three stages of the process of fruit development (14 days, 21 days, and 28 days) to study the role of lncRNAs in fruit development. The cis and trans lncRNAs were subsequently predicted and identified to determine their target genes. Notably, 1716 high-confidence lncRNAs were obtained in the three groups. A subsequent differential expression analysis of the lncRNAs between the three groups revealed 388 differentially expressed lncRNAs. A total of 11 genes were analyzed further to validate the transcriptome sequencing results. Interestingly, the MELO3C001376.2 and MSTRG.571.2 genes were found to be significantly (P < 0.05) downregulated in the fruits. This study provides a basis to better understand the functions and regulatory mechanisms of lncRNAs during the development of melon fruit.

Sugars are considered primary metabolites that determine the flavor and quality of grape berries, also playing a crucial role in the plants to resist stress. Sugars Will Eventually be Exported Transporters (SWEETs) gene family has been previously reported to be involved in the growth and development of grape, while the changes in transcriptional levels under water stress remain unclear. In this study, sixteen grape SWEETs members were identified and annotated based on their homologous genes in Arabidopsis and tomato, they were classified into four clades (Clades I to IV) with VvSWEETs by phylogenetic analysis. The highly conserved motifs and gene structures of VvSWEETs indicate that they are closely evolutionary conservation. Chromosomal localization and synteny analysis found that VvSWEETs were unevenly distributed on 11 chromosomes, and the VvSWEET5a, VvSWEET5b, VvSWEET14b and VvSWEET14c existed a relatively recent evolutionary relationship. Promoter cis-acting elements showed that the clade III has more ABRE motif, especially the VvSWEET14a. The regulation of VvSWEETs is mainly influenced by the Dof and MYB families, which are associated with grape ripening, while VvSWEET14a is closely related to the bHLH, MYB, NAC, and bZIP families. RT-qPCR data and subcellular localization show that VvSWEET14a was highly induced under early water stress and is located in the vacuole membrane. The instantaneous transformation assay identified that this gene could promote to transport hexose in the vacuole to maintain normal osmotic pressure. In summary, our study provides a basis for further research on SWEET genes function and regulatory mechanism in the future, and lays the foundation for stress resistance breeding of Vitis vinifera.

Soil salinization is one of the major environmental factors that restrict plant growth and development. Zeaxanthin epoxidase (ZEP) functions in ABA biosynthesis and the xanthophyll cycle and has a vital role in plant responses to various environmental stresses. It was found by quantitative real-time PCR (qRT-PCR) that MhZEP responded to saline-alkali stress and showed the highest expression at 48 h of saline-alkali stress, which was 14.53-fold of 0 h. The MhZEP gene was cloned from the apple rootstock begonia (Malus halliana Koehne) and its protein physicochemical properties were analyzed. Subsequently, the functional characterization of MhZEP (ID: 103403091) was further investigated in Arabidopsis thaliana. The MhZEP contained a complete open reading frame with a length of 1998 bp, and encoded 665 amino acids with an isoelectric point of 7.18. Phylogenetic tree analysis showed that MhZEP was the most homologous and closely related to Glycine max. Compared with wild-type, transgenic plants grew better under saline-alkali stress and the MhZEP-OE line showed higher chlorophyll content, carotenoid content, enzyme activities (POD, SOD, CAT and APX) and K⁺ content, whereas they had lower chlorosis and Na⁺ content than the wild type (WT), which indicated that they had strong resistance to stress. The expression levels of saline-alkali stress-related genes in A. thaliana MhZEP-OE were examined by qRT-PCR, and it was found that the MhZEP improved the tolerance of A. thaliana to saline-alkali stress tolerance by regulating the expression of carotenoid synthesis genes (MhPSY, MhZDS, MhLYCB and MhVDE) and ABA biosynthesis genes (MhNCED5, MhABI1 and MhCYP707A2). And the potassium-sodium ratio in the cytoplasm was increased to maintain ionic homeostasis by modulating the expression of Na⁺ transporter genes (MhCHX15 and MhSOS1) and K⁺ transporter genes (MhHKT1;1, MhNHX1 and MhSKOR1). Moreover, the expression of H⁺-ATPase genes (MhAHA2 and MhAHA8) was increased to reduce the oxidative damage caused by saline-alkali stress. In summary, MhZEP acted as an essential role in plant resistance to saline-alkali stress, which lays the foundation for further studies on its function in apple.

The GDSL gene family plays diverse roles in plant growth and development. Despite its significance, the functions of the GDSL in the pitaya plant are still unknown. Pitaya (Selenicereus undatus L.) also called Hylocereus undatus (Hu), belongs to the family Cactaceae and is an important tropical plant that contains high dietary fibers and antioxidants. In the present investigation, we screened 91 HuGDSL genes in the pitaya genome by conducting a comprehensive computational analysis. The phylogenetic tree categorized HuGDSL genes into 9 distinct clades in combination with four other species. Further, 29 duplicate events were identified of which 12 were tandem, and 17 were segmental. The synteny analysis revealed that segmental duplication was more prominent than tandem duplication among these genes. The majority of duplicated gene pairs (95%) indicate their Ka/Ks ratios ranging from 0.1 to 0.3, which shows that maximum HuGDSL genes were under purifying selection pressure. The cis-acting element in the promotor region contains phytohormones such as auxin, gibberellin, jasmonic acid, and abscisic acid abundantly. Finally, the HuGDSL gene expression pattern under single and multiple stresses was analyzed via; RNA-seq. We select ten stress-responsive HuGDSL genes for RT-qPCR validation. After careful investigation, we identified five HuGDSL candidate genes (HuGDSL-1/3/55/59, and HuGDSL-78) based on RNA-seq, and RT-qPCR data that showed enhanced expression in stress and melatonin-applied seedlings. This study represents valuable insights into maintaining pitaya growth and development by preparing stress-resilient pitaya genotypes through modern biotechnological techniques.

Herbaceous peony (Paeonia lactiflora Pall.) is a widely used famous traditional flower in China. It prefers cold and cool climate, but is not resistant to high temperature during summer in the middle and lower reaches of the Yangtze River. Previously, we found peroxidase (POD) is an important antioxidant enzyme that played an important role in high-temperature tolerance of P. lactiflora. The present study isolated the candidate gene PlPOD45 and verified its function in resisting high-temperature stress. And the results showed that PlPOD45 had an open reading frame of 978 bp that encoded 325 amino acids. Its protein was localized to the cell membrane and cytoplasm. High-temperature stress induced PlPOD45 expression. Heterologous overexpression of PlPOD45 improved plant tolerance to high-temperature stress, decreased reactive oxygen species (ROS) accumulation, relative electrical conductivity and malondialdehyde content, and increased the ratio of variable fluorescence to highest fluorescence and POD activity. Conversely, silencing PlPOD45 in P. lactiflora could decrease POD activity, ROS scavenging capability and cell membrane stability when these plants were exposed to high-temperature stress. These results suggest that PlPOD45 positively regulates high-temperature tolerance through ROS scavenging, which would provide a theoretical basis for improving high-temperature tolerance in P. lactiflora.

Among plants' transcription factor families, the bHLHs family has a significant influence on plant development processes and stress tolerance. However, there have been no relevant studies performed on the bHLHs family in kenaf (Hibiscus cannabinus L). Here, the bHLH transcription factors in kenaf were found using bioinformatics, and a total of 141 kenaf HcbHLH transcription factors were identified. Phylogenetic analysis revealed that these transcription factors were irregularly distributed on 18 chromosomes and separated into 20 subfamilies. Additionally, utilizing the transcriptome data under diverse abiotic pressures, the expression of HcbHLH members was analyzed under different stress conditions. A typical HcbHLH abiotic stress transcription factor, HcbHLH88, was exposed to salt, drought, heavy metals, and ABA. The findings revealed that HcbHLH88 might be activated under salt, drought, cadmium stress, and ABA conditions. Furthermore, HcbHLH88's function under salt stress conditions was studied after it was silenced using the virus-induced gene silencing (VIGS) technique. Reduced antioxidant enzyme activity and stunted plant development were seen in VIGS-silenced seedlings. Stress-related genes were shown to be considerably downregulated in the HcbHLH88-silenced kenaf plants, according to the qRT-PCR study. In conclusion, this study provides the first systematic gene family analysis of the kenaf bHLH gene family and provides a preliminary validation of the salt tolerance function of the HcbHLH88 gene. This study lays the foundation for future research on the regulatory mechanisms of bHLH genes in response to abiotic stresses.

Increasing seawater influence in coastal areas is an ongoing environmental issue. Gardening is a widespread activity mainly in touristic areas such as the Mediterranean coasts. However, the use of exotic species well adapted to salinity encompasses the risk of invasive species introduction. This study aimed to evaluate salinity tolerance of native geophytes, Pancratium maritimum L. and Eryngium maritimum L., to assess their use as ornamental species in salt affected coastal areas. Experiments were conducted using cultivated plants for flowering response and physiological and enzymatic antioxidant response. Six treatments were applied for two months, exposing plants to seawater (SW) dilutions (Tap-Water, 6.25%SW, 12.5%SW, 25%SW, 50%SW and 100%SW). Taxa decreased inflorescence production being this effect more architectonical in E. maritimum and affecting all inflorescence integrity in P. maritimum. Flowering time was strongly delayed and reduced in P. maritimum, while E. maritimum showed smaller effects among treatments. Physiological and biochemical response showed at moderate salinity levels (1/4SW) variation concomitant with late stress response and senescence in P. maritimum, with decreased water use efficiency, NPQ values, and enzymatic activity, and increased malondialdehyde (MDA) levels. In contrast, E. maritimum showed early stress response with steady gas exchange response, increasing NPQ values and catalase (CAT) and superoxide dimutase (SOD) activity, and decreasing MDA levels with salinity. Glutathione enzymes showed limited participation in both species. The results of this study suggest that neither species can be classified as halophytes, but they exhibit tolerance to low and moderate salinity levels, making them suitable for ornamental use.

Seed size is an important agronomic trait that indicates seed quality. In legumes, pods with equal and larger seeds remain the first preference of farmers and consumers. Genetic understanding related to seed size including seed allometric traits has been limited in the case of peas. To fill this void the findings presented here used the genome-wide association studies (GWAS) to identify novel candidate gene(s) putatively linked with seed size in Pisum sativum L. The study was conducted on 240 Pea Single Plant Plus Collection (PSPPC) panels of pea germplasm. Allometric traits measured included seed_length, seed_width, seed_thickness, seed_volume, seed_biomass, and seed_biomass by volume (SB_V). GWAS was performed using the Genome Association and Prediction Integrated Tool (GAPIT) on R-studio. The Bayesian information and Linkage-disequilibrium Iteratively Nested Keyway (BLINK) model provided significant single nucleotide polymorphisms (SNPs) linked with all the seed allometric traits. When analyzed the genomic regions of these SNPs provided a list of candidate genes that may be related to seed size. The present study thus provides a list of significant SNPs and relevant genes viz. Psat2g072000 for seed_length, Psat4g104320 for seed_width, Psat6g125800 and Psat6g125840 for seed_thickness, Psat6g228320 for seed_volume, Psat2g143920 for seed_biomass, and Psat2g120400 for SB_V which may prove useful in the improvement of pea seed size using breeding programs or CRISPR intervention. Understanding the genetic basis of seed size could lead to crop development with desirable seed characteristics, such as equal and larger-sized seeds with maximum yield and higher nutritional content.