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The role of halotolerant N-fixing bacteria on rice agronomic traits on saline soils by path analysis 通过通径分析耐盐固氮细菌对盐碱地水稻农艺性状的影响
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-01-20 DOI: 10.17221/386/2022-pse
M. Setiawati, B. Fitriatin, D. Herdiyantoro, Toto Bustomi, F. H. Khumairah, Nicky Oktav Fauziah, T. Simarmata
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引用次数: 1
Organic fertilization induces changes in soil nitrogen mineralization and enzyme activities 有机肥对土壤氮矿化和酶活性的影响
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-01-11 DOI: 10.17221/274/2022-pse
M. Yagüe, Carmen Lobo, P. García
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引用次数: 1
Production of lipase by Bacillus altitudinis isolated from tannery effluent 制革废水中分离的高原芽孢杆菌生产脂肪酶的研究
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.25252/se/2023/222717
Nida Tanweer, Rimsha Dilshad, R. Batool, N. Jamil
Lipases are important enzymes involved in hydrolytic processes that bring about the breakdown of triacylglycerol to free fatty acids and glycerol. Microbial lipases have diverse applications in the various biotechnological fields such as tannery, food, detergent and pharmaceutical industries. The main purpose of this study was to isolate and characterize the lipase-producing bacteria from the effluent of a tannery located in Kasur, Pakistan. Eight bacterial strains L1, L3, L5, L6, L9, L10, L11, and L12 were isolated, followed by screening on Tributyrin Agar Medium supplemented with mustard oil. These strains were morphologically and biochemically characterized. Growth kinetic studies of these bacteria showed optimum growth at 37°C and pH 7. Sucrose, yeast extract, and olive oil were found best as carbon, nitrogen, and oil source, respectively. The bacterial strains were non- halophilic as they preferred to grow at a low salt concentration (3%). Both of the strains L5 and L10 showed good lipase activity (99 and 92U/mL) and lipase enzyme of these strains was thermostable till 67ºC with a high protein content of 321.32 and 307.02 mg/mL, respectively. The molecular weight of lipase produced by L5 and L10 varied from 32-52kDa. Among the isolated bacterial strains, two strains L5 and L10 showed high lipase activity. 16S rRNA sequencing revealed that L5 and L10 strains had 99% homology with Bacillus altitudinis. This study signifies that the tanneries can be a good source of lipase-producing bacteria and Bacillus altitudinis can be a good lipase producer.
脂肪酶是参与水解过程的重要酶,水解过程将三酰基甘油分解为游离脂肪酸和甘油。微生物脂肪酶在制革、食品、洗涤剂和制药等生物技术领域有着广泛的应用。本研究的主要目的是从位于巴基斯坦卡苏尔的一家制革厂的废水中分离和表征脂肪酶生产细菌。分离出L1、L3、L5、L6、L9、L10、L11、L12 8株菌株,在添加芥末油的Tributyrin琼脂培养基上进行筛选。这些菌株的形态和生化特征。这些细菌的生长动力学研究表明,在37°C和pH 7的条件下生长最佳。蔗糖、酵母浸膏和橄榄油分别是最佳的碳源、氮源和油源。菌株是非嗜盐的,因为它们喜欢在低盐浓度(3%)下生长。菌株L5和L10均表现出良好的脂肪酶活性(分别为99和92U/mL),且脂肪酶耐热至67℃,蛋白质含量分别为321.32和307.02 mg/mL。L5和L10产生的脂肪酶分子量从32-52kDa不等。其中,菌株L5和L10表现出较高的脂肪酶活性。16S rRNA测序结果显示,L5和L10菌株与高原芽孢杆菌同源性达99%。该研究表明,制革厂可以成为脂肪酶产生菌的良好来源,而高原芽孢杆菌可以成为脂肪酶产生菌的良好来源。
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引用次数: 0
Efficiency of selenium biofortification of spring wheat: the role of soil properties and organic matter amendment 春小麦硒生物强化效率:土壤性质和有机质修正的作用
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-12-15 DOI: 10.17221/357/2022-pse
Tomáš Mrština, Lukáš Praus, L. Kaplan, J. Száková, P. Tlustoš
The effect of soil selenate application to two different soils (Phaeozem and Cambisol) on biomass yield and selenium (Se) uptake by spring wheat (Triticum aestivum L.) was investigated in a pot experiment. Additionally, organic amendment (fugate, i.e. liquid by-product from the biogas plant) was applied to assess (i) the effect of organic matter on the bioavailability of Se and (ii) the fugate (containing 2.3 mg/kg of Se) as a potential source of Se for plants. Selenium was applied at two levels: 6.4 µg/kg (Se1) and 32 µg/kg (Se2) of soil. The efficiency of biofortification and the distribution of selenium within individual plant compartments were assessed in this case. The highest Se contents in the grain were achieved in the treatments receiving NPK fertiliser together with selenate, 455 µg/kg (Se1) and 2 721 µg/kg (Se2) when wheat was planted in Phaeozem. Fugate in co-application with selenate significantly reduced Se content in wheat plants as compared to treatments enriched solely with selenate. The lower Se contents in the wheat plants growing in Phaeozem were due to the biodilution effect, whereas in Cambisol, the decrease in wheat Se uptake was not clearly driven by a particular factor.
在盆栽试验中,研究了土壤硒酸盐在两种不同土壤(黄土和棕壤)中的施用对春小麦生物量产量和硒吸收的影响。此外,应用有机改良剂(逸出物,即沼气厂的液体副产物)来评估(i)有机物对Se生物利用度的影响,以及(ii)逸出物(含有2.3mg/kg Se)作为植物Se的潜在来源。硒的施用量分为两个水平:6.4微克/公斤(Se1)和32微克/千克(Se2)的土壤。在这种情况下,评估了生物强化的效率和硒在各个植物区室中的分布。当小麦种植在黄钙土中时,在施用NPK肥料和硒酸盐的处理中,谷物中的Se含量最高,分别为455µg/kg(Se1)和2721µg/kg(Se2)。与单独富含硒酸盐的处理相比,与硒酸盐共同施用的富格酸盐显著降低了小麦植株中的硒含量。生长在黄钙土中的小麦植株中硒含量较低是由于生物溶解作用,而在寒地,小麦硒吸收的减少并不是由特定因素引起的。
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引用次数: 1
Effect of exogenous selenium on mineral nutrition and antioxidative capacity in cucumber (Cucumis sativus L.) seedlings under cadmium stress 外源硒对镉胁迫下黄瓜幼苗矿质营养和抗氧化能力的影响
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-12-12 DOI: 10.17221/294/2022-pse
Hongyan Sun, Xiaoyun Wang, Ni Yang, Huanxin Zhou, Yifang Gao, Yu Jia, Xiaoxiao Wang
The ameliorative effects and its mitigation mechanisms of selenium (Se) on cadmium (Cd) toxicity in cucumber seedlings were studied through hydroponic experiments. Cd and other mineral nutrient concentrations, antioxidant enzyme activities, and antioxidant contents in cucumber were studied. The results revealed that exogenous Se significantly decreased the Cd concentrations in all tissues, especially in the leaves. Moreover, exogenous Se (Cd + Se) could increase Zn, Na, leaf Cu, stem/root Fe, stem/root Ca, and stem/root Mg concentrations; and reduce leaf Mg concentration, compared with Cd alone treatment. Additionally, the application of Se ameliorated the toxicity of Cd by harmonising the activities of antioxidase, such as Cd + Se treatment reduced Cd-induced increase of superoxide dismutase, glutathione peroxidase, leaf/stem ascorbate peroxidase (APX) activities, which resulted in the significant decrease of the content of hydrogen peroxide, and malondialdialdehyde; increased root APX, and glutathione reductase activities. In addition, the content of nonenzymatic antioxidants such as root-reduced glutathione and oxidised glutathione was significantly increased by adding Se under Cd stress. Also, exogenous Se enhanced the total antioxidant capacity in terms of cupric-reducing antioxidant capacity and decreased total phenols, flavonoids, and leaf/root proline contents under Cd stress. In general, 3 μmol/L Se was conducive to plant growth and improved the cucumber’s ability to alleviate Cd stress.
通过水培试验,研究了硒(Se)对黄瓜幼苗镉(Cd)毒性的改善作用及其缓解机制。研究了黄瓜中Cd等矿质营养元素的含量、抗氧化酶活性和抗氧化剂含量。结果表明,外源硒显著降低了各组织中Cd的浓度,尤其是叶片中Cd的含量。此外,外源Se(Cd+Se)可增加Zn、Na、叶片Cu、茎/根Fe、茎/根Ca和茎/根Mg的浓度;并降低叶片Mg浓度。此外,硒的施用通过协调抗氧化酶的活性来改善镉的毒性,如Cd+Se处理降低了镉诱导的超氧化物歧化酶、谷胱甘肽过氧化物酶、叶/茎抗坏血酸过氧化物酶(APX)活性的增加,导致过氧化氢和丙二醛的含量显著降低;根APX和谷胱甘肽还原酶活性增加。此外,在镉胁迫下,添加硒可显著提高根还原型谷胱甘肽和氧化型谷胱甘肽等非酶抗氧化剂的含量。此外,外源硒在镉胁迫下提高了总抗氧化能力,降低了总酚、黄酮类化合物和叶/根脯氨酸含量。总的来说,3μmol/L Se有利于植物生长,提高了黄瓜对Cd胁迫的缓解能力。
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引用次数: 0
Exogenous glucose modulated the diversity of soil nitrogen-related bacteria and promoted the nitrogen absorption and utilisation of peanut 外源葡萄糖调节了土壤氮相关细菌的多样性,促进了花生对氮的吸收和利用
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-12-09 DOI: 10.17221/275/2022-pse
Haiyan Liang, Liyu Yang, Qi Wu, L. Yin, Cuiping Meng, Pu Shen
Exogenous carbon (C) not only regulates plant growth but also provides energy for microbes and improves the soil environment. We hypothesised that exogenous C could improve plant growth by affecting the soil environment. Therefore, pot experiments were conducted and peanut cvs. Huayu 22(H) and NN-1(B) were used under three different treatments (the control, single nitrogen (N), and N combined with glucose (CN)). The results showed that the abundance and diversity of N-fixing bacteria are obviously influenced by the C and N, and exogenous C can promote the restoration of microbial diversity. The relative abundances of Burkholderiales were increased under HCN and BCN to 9.8% and 9.5%, respectively, compared to the control (3.9%, 2.5%). The abundance of N fixation bacteria increased mainly due to the soil nutrient change. In comparison with the single N treatment, the addition of the C significantly decreased the soil NH4+-N and NO3–-N contents by 31.0% and 13.3%, respectively. And the activities of soil urease and nitrogenase were significantly increased. Compared to the control, single N significantly limited the root development, while the addition of C played a promoting role in root growth. Plant N accumulation increased compared with the control, but there was no significant difference between N treatment and CN treatment. These results indicated that exogenous C promoted soil microorganism activity and strengthened plant growth by changing the soil environment.
外源碳(C)不仅可以调节植物生长,还可以为微生物提供能量,改善土壤环境。我们假设外源C可以通过影响土壤环境来促进植物生长。因此,对花生进行盆栽试验。花育22号(H)和NN-1(B)分别在对照、单施氮(N)和氮与葡萄糖(CN) 3种不同处理下处理。结果表明,固氮细菌的丰度和多样性明显受C和N的影响,外源C可以促进微生物多样性的恢复。HCN和BCN处理下Burkholderiales的相对丰度分别比对照(3.9%、2.5%)提高了9.8%和9.5%。固氮细菌丰度的增加主要是由于土壤养分的变化。与单施氮处理相比,施C显著降低了土壤NH4+-N和NO3——N含量,分别降低了31.0%和13.3%。土壤脲酶和氮酶活性显著提高。与对照相比,单施氮显著限制了根系发育,而添加C对根系生长有促进作用。与对照相比,植株氮素积累量增加,但氮素处理与CN处理间差异不显著。上述结果表明,外源C通过改变土壤环境,促进了土壤微生物活性,促进了植物生长。
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引用次数: 0
Grain yield and quality of wheat in wheat-legumes intercropping under organic and conventional growing systems 有机和常规种植系统下麦-豆间作小麦的产量和品质
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-11-30 DOI: 10.17221/276/2022-pse
P. Dvořák, I. Capouchová, M. Král, P. Konvalina, D. Janovská, Matěj Satranský
The effect of simultaneous intercropping of winter wheat (Triticum aestivum L.) with different legumes of faba bean (Vicia faba L.), pea (Pisum sativum L.), and purple clover (Trifolium incarnatum L.) on selected production and qualitative parameters of wheat was evaluated in field trials conducted both in organic and conventional cropping systems, in comparison with pure sown wheat. Wheat intercropped with legumes achieved higher grain yield compared to pure sown wheat for an average of two years. However, in organic cultivation, the positive effect of intercropping on wheat yields was more pronounced. In addition, a strong influence of the year was noted. In 2021, in the organic cropping system, the most yielding intercropped wheat (especially with pea and bean) achieved114–117% higher yields compared to pure sown wheat (in the previous year of 2020, it was usually only about 102–106%). In the conventional cropping system, the effect of intercropping on wheat yield was significantly weaker, and in 2021, wheat intercropped with legumes reached even lower yields than pure sown wheat in some cases. In terms of sowing methods (both in organic and conventional cropping systems), mixed sowing with individual legumes significantly exceeded the yields of wheat grown with legumes in separate, alternating rows. As regards quality parameters, wheat intercropped with legumes reached in comparison with pure sown wheat usually had higher crude protein content in wheat grain dry matter and higher values of Zeleny sedimentation.
在有机和常规种植系统中进行的田间试验中,与纯播种小麦相比,评估了冬小麦(Triticum aestivum L.)与蚕豆(Vicia faba L.)、豌豆(Pisum sativum L.)和紫三叶草(Trifolium incarnatum L.)等不同豆类同时间作对小麦产量和质量参数的影响。与纯播种小麦相比,豆类间作小麦平均两年的粮食产量更高。然而,在有机栽培中,间作对小麦产量的积极影响更为明显。此外,还注意到该年的强烈影响。2021年,在有机种植系统中,与纯播种小麦相比,产量最高的间作小麦(尤其是豌豆和豆类)的产量提高了114-117%(2020年前一年,通常只有102-106%左右)。在常规种植制度中,间作对小麦产量的影响明显较弱,2021年,与豆类作物间作的小麦产量在某些情况下甚至低于纯播种小麦。就播种方法而言(在有机和传统种植系统中),与单个豆类混合播种的产量大大超过了与豆类分行交替种植的小麦的产量。在品质参数方面,与纯播小麦相比,间作豆类小麦通常具有较高的小麦籽粒干物质粗蛋白含量和较高的Zeleny沉降值。
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引用次数: 1
Soil weed seedbank under different cropping systems of middle Indo-Gangetic Plains 印度-恒河平原中部不同种植制度下的土壤杂草种子库
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-11-28 DOI: 10.17221/162/2022-pse
Prashant Sharma, M. Singh, K. Verma, S. K. Prasad
Trees on agricultural fields can have a positive or negative impact on weed seedbank (WSB) due to diverse environmental and soil characteristics. Therefore, soil samples were drawn in six cropping systems [two agroforest systems (AFS): guava, mango; three horticulture systems (HCS): guava, mango, Indian gooseberry; and annual crop system (ACS)] at two landscape positions (lowland and upland) and two soil depths (0–15 cm and 15–30 cm) using factorial randomised block design each replicated three times. Results showed that guava-AFS had the highest WSB of different categories in general and individual weed species in particular, except for Eragrostis pilosa and Dactyloctenium aegyptium. Simultaneously, guava-AFS also showed the maximum Shannon-Weaver, species richness and Simpson index and was low in Whittaker statistics (βW). The species evenness varied non-significantly with the cropping systems. Similarly, the landscape position had no discernible effect on any weed diversity indices; however lowland landscape position was dominated by Cyperus spp. and E. pilosa, while the upland by Phyllanthus niruri. Furthermore, with the exception of βW, the WSB and diversity indices were found to be higher on the topsoil (0–15 cm). Our study establishes that the AFS system in the semi-arid sub-tropics has a more diverse WSB indicating a healthy system, as opposed to HCS, which has a dominance of certain weed species, opening the door for more severe infestation of invasive weed species.
由于不同的环境和土壤特征,农田树木对杂草种子库的影响可能是积极的,也可能是消极的。因此,在6种种植系统[两种农林复合系统(AFS):番石榴、芒果;三大园艺体系(HCS):番石榴、芒果、印度醋栗;在两个景观位置(低地和高地)和两个土壤深度(0-15厘米和15-30厘米)使用因子随机区组设计,每个重复三次。结果表明,番石榴- afs在不同种类杂草的WSB中总体最高,个别杂草的WSB最高,除油葵(Eragrostis pilosa)和埃及Dactyloctenium aegyptium外。同时,番石榴的Shannon-Weaver、物种丰富度和Simpson指数最高,Whittaker统计值(βW)较低。树种均匀度随种植制度变化不显著。同样,景观位置对杂草多样性指数的影响也不明显;低地景观位置以莎草(Cyperus spp.)和金针叶草(E. pilosa)为主,而高地景观位置以千叶草(Phyllanthus niruri)为主。此外,除βW外,表层土壤(0 ~ 15 cm) WSB和多样性指数均较高。我们的研究表明,在半干旱的亚热带地区,AFS系统具有更多样化的WSB,这表明该系统是一个健康的系统,而HCS则具有某些杂草的优势,这为入侵杂草的更严重侵袭打开了大门。
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引用次数: 1
Effects of various poppy seed pre-sowing treatments on the dynamics of field emergence, structure of yield parameters, oil content and yield of seed 不同播前处理对罂粟出苗动态、产量参数结构、含油量和种子产量的影响
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-11-28 DOI: 10.17221/364/2022-pse
Matěj Satranský, I. Capouchová, B. Burešová, P. Procházka
The effects of various pre-sowing treatments of poppy seed (chemical product Cruiser OSR, stimulation products TS Osivo and Enviseed, physical treatment by E-ventus method, biological products Polyversum and Gliorex) on dynamics of field emergence, seed yield, and structure of yield parameters were evaluated in two cultivars grown in three-year trials. Seed treatment with TS Osivo and Enviseed led to a significant increase in field emergence compared to the untreated control and was on the same level as the Cruiser OSR-treated variant. In variants treated with Polyversum, Gliorex, and E-ventus, the number of emerged plants was noticeably lower. The stand density was a key factor in achieving a satisfactory yield, given that in the structure of other yield parameters (number of capsules per plant, weight of seed per capsule, and thousand seed weight), the differences between the variants were lower and often insignificant. The average yield ranged from 0.85 t/ha (Polyversum) to 1.39 t/ha (Cruiser OSR), and the stimulant seed treatments were also proved to be effective (TS Osivo 1.23 t/ha and Enviseed 1.16 t/ha). Variants with biological and physical treatment did not differ significantly from the control (0.88 t/ha) in average yield. All parameters evaluated were strongly affected by the year (weather conditions).
以2个品种为试验材料,评价了不同播前处理(化学制剂Cruiser OSR、刺激制剂TS Osivo和Enviseed、E-ventus法物理处理、生物制剂Polyversum和Gliorex)对田间出苗动态、种子产量和产量参数结构的影响。与未处理的对照相比,使用TS Osivo和Enviseed处理的种子显著增加了田间出苗率,与Cruiser osr处理的变种相同。在用Polyversum、Gliorex和E-ventus处理的变异中,出现的植株数量明显较低。在其他产量参数(单株蒴果数、每粒蒴果重和千粒重)的结构上,各变异间的差异较小,且往往不显著,因此林分密度是获得满意产量的关键因素。平均产量从0.85 t/ha (Polyversum)到1.39 t/ha (Cruiser OSR)不等,刺激性种子处理也被证明是有效的(TS Osivo 1.23 t/ha和Enviseed 1.16 t/ha)。生物处理和物理处理变异的平均产量与对照(0.88 t/公顷)差异不显著。所有评估的参数都受到年份(天气条件)的强烈影响。
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引用次数: 0
Variability of cannabinoid yields of fibre hemp cultivars depending on the sowing density and nitrogen fertilisation 麻纤维品种大麻素产量随播种密度和氮肥的变化
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2022-11-22 DOI: 10.17221/223/2022-pse
J. Poniatowska, K. Panasiewicz, M. Szalata, Livia Zarina, S. Zute, K. Wielgus
The aim of the experiments was to determine the effect of sowing density and nitrogen fertilisation on the cannabidiol (CBD) and Δ9-tetrahydrocannabinol (Δ9-THC) yields in selected cultivars of fibre hemp. A three-factor field experiment was conducted in 2014–2016. Factor A: cultivar (Futura 75, KC Dora and Tygra), factor B: sowing density (60 and 180 seeds/m2) and factor C: nitrogen fertilisation (0, 30, 60 and 90 kg/ha). The CBD yield ranged from 10.52 g/m2 in cv. Tygra to 10.99 g/m2 in cv. KC Dora. Among the examined cultivars, the highest yield of Δ9-THC in cv. KC Dora was observed. Sowing density did not modify the CBD yield, but increasing the density from 60 to180 seeds/m2 caused an increase in the yield of Δ9-THC in cvs. KC Dora and Tygra. Nitrogen fertilisation significantly influenced the yields of CBD and Δ9-THC. In both cases, yield increased until the rate of 60 kg N/ha.
试验的目的是确定播种密度和氮肥对所选纤维大麻品种大麻二酚(CBD)和Δ9-四氢大麻酚(Δ9-THC)产量的影响。2014-2016年进行了一项三因素现场试验。因子A:栽培品种(Futura 75、KC Dora和Tygra),因子B:播种密度(60和180种子/m2),因子C:氮肥(0、30、60和90公斤/公顷)。CBD产量范围从Tygra的10.52 g/m2到KC Dora的10.99 g/m2。在试验品种中,Δ9-THC在KC-Dora中的产量最高。播种密度不会改变CBD产量,但将密度从60粒/m2增加到180粒/m2会使cvs中Δ9-THC的产量增加。KC多拉和泰格拉。施氮显著影响CBD和Δ9-THC的产量。在这两种情况下,产量都有所增加,直到达到每公顷60公斤的产量。
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引用次数: 2
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