Pub Date : 2023-08-01DOI: 10.1007/s11816-023-00847-4
Ilchan Song, H. Jeon, Ryza A Priatama, S. Gayathri, K. Ko, Young Koung Lee
{"title":"Effect of plasma-activated water on peanut seed germination and vegetative growth in a hydroponic system","authors":"Ilchan Song, H. Jeon, Ryza A Priatama, S. Gayathri, K. Ko, Young Koung Lee","doi":"10.1007/s11816-023-00847-4","DOIUrl":"https://doi.org/10.1007/s11816-023-00847-4","url":null,"abstract":"","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"17 1","pages":"573 - 583"},"PeriodicalIF":2.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43696232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-27DOI: 10.1007/s11816-023-00848-3
Bumkyu Lee, S. Sohn
{"title":"Development of chloroplast DNA markers for environmental monitoring of genetically modified Brassica napus and Brassica hybrids","authors":"Bumkyu Lee, S. Sohn","doi":"10.1007/s11816-023-00848-3","DOIUrl":"https://doi.org/10.1007/s11816-023-00848-3","url":null,"abstract":"","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"17 1","pages":"489 - 497"},"PeriodicalIF":2.4,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43669095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-27DOI: 10.1007/s11816-023-00849-2
Yun-Jeong Han, Ha-Young Kang, Young Soon Kim, Jeong-Il Kim
{"title":"Functional divergence of two closely related carboxylesterases in pepper (Capsicum annuum L.)","authors":"Yun-Jeong Han, Ha-Young Kang, Young Soon Kim, Jeong-Il Kim","doi":"10.1007/s11816-023-00849-2","DOIUrl":"https://doi.org/10.1007/s11816-023-00849-2","url":null,"abstract":"","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"17 1","pages":"499 - 507"},"PeriodicalIF":2.4,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47238052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-22DOI: 10.1007/s11816-023-00846-5
S. Heo, Suna Chung
{"title":"Development of phenomic approaches to estimate harvesting time of Artemisia argyi and classifying its quality based on flavonoids, essential oil and leaf weight ratio","authors":"S. Heo, Suna Chung","doi":"10.1007/s11816-023-00846-5","DOIUrl":"https://doi.org/10.1007/s11816-023-00846-5","url":null,"abstract":"","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"17 1","pages":"459 - 469"},"PeriodicalIF":2.4,"publicationDate":"2023-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41544239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-19DOI: 10.1007/s11816-023-00844-7
C. Lee, Jong-Seop Lee, H. Chung, D. Lee, Jong-Seok Lim, Kibum Kim, Jin Wook Kim, Y. S. Lee, Kyung Soo Kim, H. Min, K. Ko, S. Myung
{"title":"Expression and in vitro function of anti-PD-L1 human antibody expressed in plant","authors":"C. Lee, Jong-Seop Lee, H. Chung, D. Lee, Jong-Seok Lim, Kibum Kim, Jin Wook Kim, Y. S. Lee, Kyung Soo Kim, H. Min, K. Ko, S. Myung","doi":"10.1007/s11816-023-00844-7","DOIUrl":"https://doi.org/10.1007/s11816-023-00844-7","url":null,"abstract":"","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"17 1","pages":"531 - 539"},"PeriodicalIF":2.4,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46825446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-07DOI: 10.1007/s11816-023-00842-9
N. Mohammed, M. Afzal, Sulieman A. Al-Faifi, M. A. Khan, Y. Refay, Bazel H. AL-Samin, S. Alghamdi, Abdullah Ibrahim
{"title":"Effectiveness of sequence-related amplified polymorphism (SRAP) markers to assess the geographical origin and genetic diversity of collected lentil genotypes","authors":"N. Mohammed, M. Afzal, Sulieman A. Al-Faifi, M. A. Khan, Y. Refay, Bazel H. AL-Samin, S. Alghamdi, Abdullah Ibrahim","doi":"10.1007/s11816-023-00842-9","DOIUrl":"https://doi.org/10.1007/s11816-023-00842-9","url":null,"abstract":"","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"17 1","pages":"519 - 530"},"PeriodicalIF":2.4,"publicationDate":"2023-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46568955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-29DOI: 10.1007/s11816-023-00841-w
Ashwini M. Darshetkar, Swaranjali S. Patil, A. Pable, A. Nadaf, V. Barvkar
{"title":"Loss of mitogenome-encoded respiratory genes complemented by nuclear transcripts in halophyte Pandanus odorifer (Forssk.) Kuntze","authors":"Ashwini M. Darshetkar, Swaranjali S. Patil, A. Pable, A. Nadaf, V. Barvkar","doi":"10.1007/s11816-023-00841-w","DOIUrl":"https://doi.org/10.1007/s11816-023-00841-w","url":null,"abstract":"","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49086617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1007/s11816-023-00839-4
J. Kim, Mi Young Kim, Jae Hyun Lee, J. Kim, O. Lee
{"title":"Establishment of a qualified ex vitro-acclimatized whole plant reproduction system using secondary somatic embryos in Panax ginseng","authors":"J. Kim, Mi Young Kim, Jae Hyun Lee, J. Kim, O. Lee","doi":"10.1007/s11816-023-00839-4","DOIUrl":"https://doi.org/10.1007/s11816-023-00839-4","url":null,"abstract":"","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"17 1","pages":"331 - 339"},"PeriodicalIF":2.4,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46139195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.1007/s11816-023-00840-x
Nekha Johnson, Diana Rodriguez Diaz, Sivakumar Ganapathy, John S Bass, Toni M Kutchan, Abdul L Khan, Albert B Flavier
The flame lily,Gloriosa superba L., is one of the two primary sources of the anti-inflammatory drug, colchicine. Previous studies have shown that a higher level of colchicine production occurs in the rhizomes than in leaves and roots. Earlier precursor feeding and transcriptome analysis of G. superba have provided a putative pathway and candidate genes involved in colchicine biosynthesis. Comparative analysis of expression levels of candidate pathway genes in different tissues of G. superba using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) can reveal highly expressed genes in the rhizome compared to other tissues which could suggest roles of the gene products in colchicine biosynthesis. Normalization is an important step in effectively analyzing differential gene expression by qRT-PCR with broader applications. The current study selected candidate reference genes from the transcriptome datasets and analyzed them to determine the most stable genes for normalization of colchicine biosynthesis-related genes. Using RefFinder, one stable reference gene, UBC22, was selected to normalize gene expression levels of candidate methyltransferase (MT) genes in the leaves, roots, and rhizomes of G. superba. With UBC22 as reference gene, the methyltransferases, GsOMT1, GsOMT3, and GsOMT4 showed significantly higher expression levels in the rhizome of G. superba, while MT31794 was more highly expressed in the roots. In conclusion, the current results showed a viable reference gene expression analysis system that could help elucidate colchicine biosynthesis and its exploitation for increased production of the drug in G. superba.
Supplementary information: The online version contains supplementary material available at 10.1007/s11816-023-00840-x.
{"title":"Evaluation of reference genes for qRT-PCR studies in the colchicine producing <i>Gloriosa superba</i> L.","authors":"Nekha Johnson, Diana Rodriguez Diaz, Sivakumar Ganapathy, John S Bass, Toni M Kutchan, Abdul L Khan, Albert B Flavier","doi":"10.1007/s11816-023-00840-x","DOIUrl":"10.1007/s11816-023-00840-x","url":null,"abstract":"<p><p>The flame lily<b>,</b> <i>Gloriosa superba</i> L., is one of the two primary sources of the anti-inflammatory drug, colchicine. Previous studies have shown that a higher level of colchicine production occurs in the rhizomes than in leaves and roots. Earlier precursor feeding and transcriptome analysis of <i>G. superba</i> have provided a putative pathway and candidate genes involved in colchicine biosynthesis. Comparative analysis of expression levels of candidate pathway genes in different tissues of <i>G. superba</i> using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) can reveal highly expressed genes in the rhizome compared to other tissues which could suggest roles of the gene products in colchicine biosynthesis. Normalization is an important step in effectively analyzing differential gene expression by qRT-PCR with broader applications. The current study selected candidate reference genes from the transcriptome datasets and analyzed them to determine the most stable genes for normalization of colchicine biosynthesis-related genes. Using RefFinder, one stable reference gene, UBC22, was selected to normalize gene expression levels of candidate methyltransferase (MT) genes in the leaves, roots, and rhizomes of <i>G. superba</i>. With UBC22 as reference gene, the methyltransferases, GsOMT1, GsOMT3, and GsOMT4 showed significantly higher expression levels in the rhizome of <i>G. superba</i>, while MT31794 was more highly expressed in the roots. In conclusion, the current results showed a viable reference gene expression analysis system that could help elucidate colchicine biosynthesis and its exploitation for increased production of the drug in <i>G. superba</i>.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11816-023-00840-x.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":" ","pages":"1-11"},"PeriodicalIF":2.4,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10195008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10091337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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