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Effect of Cricket Powder Incorporation on the Profile of Volatile Organic Compounds, Free Amino Acids and Sensory Properties of Gluten-Free Bread 蟋蟀粉掺入对无麸质面包挥发性有机物、游离氨基酸和感官特性的影响
IF 2.4 4区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-11-22 DOI: 10.31883/pjfns/156404
M. Wieczorek, P. Kowalczewski, Natalia Drabińska, M. Różańska, H. Jeleń
Scientists around the world are interested in edible insects as a source of valuable nutrients. Among the most often described are crickets, which represent a source of significant amounts of protein, lipids, vitamins, and minerals. This article reports results of a study into the effect of starch-to--cricket powder (CP) addition on the free amino acid profile as well as potential odorants in gluten-free (GF) bread. A significant 2.6-fold increase was observed in the content of essential amino acids in the CP-enriched GF bread. Moreover, the CP addition resulted in the formation of many volatile compounds, such as pyrazines, furans, and sulfur-containing compounds, which exhibit strong aroma-enhancing properties. The attractiveness of the CP-enriched bread was confirmed by the results of the sensory analysis, showing a significant improvement in its flavor when compared to the tradi tional GF bread without CP. GF bread enriched with CP was characterized by caramel, roasty, and cooked potatoes notes. On the basis of the obtained results, it was concluded that the use of CP not only improves the nutritional value but also aroma of GF bread.
世界各地的科学家都对食用昆虫作为有价值的营养来源感兴趣。其中最常被描述的是蟋蟀,它们是大量蛋白质、脂质、维生素和矿物质的来源。本文报道了一项研究结果,研究了添加淀粉-蟋蟀粉(CP)对无谷蛋白(GF)面包中游离氨基酸谱和潜在气味的影响。富含cp的GF面包中必需氨基酸的含量显著增加2.6倍。此外,CP的加入还形成了许多挥发性化合物,如吡嗪、呋喃和含硫化合物,这些化合物具有很强的芳香增强特性。感官分析结果证实了富含CP的面包的吸引力,与不含CP的传统GF面包相比,其风味有显着改善。富含CP的GF面包具有焦糖,烘烤和熟土豆的特征。在此基础上得出结论:添加CP不仅提高了GF面包的营养价值,而且改善了其香气。
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引用次数: 5
Iron, Zinc, Copper, Manganese and Chromium in Green Teas, Their Transfer to Extracts and Correlations between Contents of Elements and Bioactive Compounds 绿茶中的铁、锌、铜、锰和铬,它们在提取物中的转移及元素含量与生物活性化合物的相关性
IF 2.4 4区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-11-22 DOI: 10.31883/pjfns/156394
M. Herman, M. Janiak, J. Sadlik, W. Piekoszewski, R. Amarowicz
determine the of transfer of selected elements, , iron (Fe), (Zn), (Cu), manga (Mn), chromium (Cr), teas to investigate whether the main bioactive compounds of the extracts transfer. Twelve commercially available green teas were used in the study. The contents of elements in green teas and their extracts obtained with 80% acetone ( v/v ) were analysed by inductively coupled plasma optical emission spectroscopy (ICP-OES). High performance liquid chromatography in reverse phase (RP-HPLC) was used to determine contents of caffeine, (–)-epigallocatechin (EGC), (–)-epicatechin (EP), (–)-epigallocatechin gallate (EGCG), and (–)-epicatechin gallate (ECG). The element with the highest content in green teas was Mn (711–1402 µg/g), but its transfer to extracts was the lowest (0.269–0.646%). The mean Fe transfer, second abundant element in teas (115–725 µg/g), was 5.52%. The contents of Mn and Fe in extracts were 5.08–30.2 and 10.7–90.1 µg/g, respectively. Zn, Cu, and Cr were transferred with means of 10.4, 20.0, and 26.2%, respectively, which resulted in their contents in the extracts in the ranges of 5.03–12.6, 1.93–13.8, and 0.128–2.03 µg/g, respectively. The significant positive correlations of Zn content in extracts and/or transfer to extracts with EGCG, EGC and total flavan-3-ols as well as between the same Fe variables and EGC were determined, which suggested that these flavan-3-ols may positively affect the transfer of Fe and Zn from green tea to extracts. In turn, significant but negative correlations were found in the case of Mn and Cu. Future research is needed to identify the causes of the various transfer rate of elements from green teas to extracts.
测定所选元素的转移量,铁(Fe)、(Zn)、(Cu)、锰(Mn)、铬(Cr)、茶的主要生物活性化合物是否转移。研究中使用了12种市售绿茶。采用电感耦合等离子体发射光谱(ICP-OES)对80%丙酮(v/v)得到的绿茶及其提取物中的元素含量进行了分析。采用反相高效液相色谱法(RP-HPLC)测定咖啡因、(-)-没食子儿茶素(EGC)、(-)-表儿茶素(EP)、(-)-没食子儿茶素没食子酸酯(EGCG)和(-)-表儿茶素没食子酸酯(ECG)的含量。绿茶中Mn的含量最高(711 ~ 1402µg/g),但其转移到提取物中的含量最低(0.269 ~ 0.646%)。茶中第二大元素铁的平均转移率为5.52%(115 ~ 725µg/g)。提取物中Mn和Fe含量分别为5.08 ~ 30.2µg/g和10.7 ~ 90.1µg/g。Zn、Cu和Cr的转移率分别为10.4、20.0和26.2%,导致其含量分别为5.03 ~ 12.6、1.93 ~ 13.8和0.128 ~ 2.03µg/g。结果表明,绿茶提取物中锌含量与EGCG、EGC、总黄酮-3-醇含量及(或)转移至提取物中,以及相同铁变量与EGC之间呈显著正相关,表明这些黄烷-3-醇可能正影响绿茶提取物中铁和锌的转移。反过来,在锰和铜的情况下发现了显著的负相关。未来的研究需要确定从绿茶到提取物的各种元素转移速率的原因。
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引用次数: 3
Health Benefits of Vegetarian and Mediterranean Diets: Narrative Review 素食和地中海饮食对健康的益处:叙述性综述
IF 2.4 4区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-11-16 DOI: 10.31883/pjfns/156067
Katarzyna Pieczyńska, P. Rzymski
Diet is an important lifestyle factor influencing disease risk. Vegetarian and Mediterranean diets have their proponents and are promoted for various potential health benefits. Over the years, numerous cross-sectional and cohort studies and randomized clinical trials have been conducted to elucidate the relationship between the Mediterranean and vegetarian diet and cardiovascular, cancer, diabetes, and other disease risks. More recently, research has been conducted to compare both diets directly. In this narrative review, we discuss the effects of vegetarian and Mediterranean diets on lipid profile, blood pressure, inflammation markers, body weight, risk of cardiovascular disease, cancer, diabetes mellitus, metabolic syndrome, and chronic kidney disease, as well as their associations with gut microbiota and mental health. The paper also discusses the studies comparing vegetarian and Mediterranean diets and their health effects. It provides further evidence that both diets can be beneficial and advocates their promotion, especially in Westernized populations plagued by various chronic lifestyle-associated diseases. At the same time, the Mediterranean dietary model may appear to be a superior public health strategy, less prone to the risk of nutritional deficiencies and less challenging in implantation on a broader scale. However, further studies based on cross-over design and long-term observations are recommended to thoroughly compare vegetarian and Mediterranean diets and draw more firm conclusions on their effects on health.
饮食是影响疾病风险的重要生活方式因素。素食和地中海饮食有其支持者,并因各种潜在的健康益处而得到推广。多年来,已经进行了大量的横断面和队列研究以及随机临床试验,以阐明地中海和素食与心血管、癌症、糖尿病和其他疾病风险之间的关系。最近,有研究直接比较了这两种饮食。在这篇叙述性综述中,我们讨论了素食和地中海饮食对血脂、血压、炎症标志物、体重、心血管疾病风险、癌症、糖尿病、代谢综合征和慢性肾脏疾病的影响,以及它们与肠道微生物群和心理健康的关系。本文还讨论了素食和地中海饮食的比较研究及其对健康的影响。它提供了进一步的证据,证明这两种饮食都是有益的,并提倡推广它们,尤其是在受各种慢性生活方式相关疾病困扰的西化人群中。与此同时,地中海饮食模式似乎是一种优越的公共卫生策略,不太容易出现营养缺乏的风险,在更广泛的范围内植入也不那么具有挑战性。然而,建议基于交叉设计和长期观察进行进一步研究,以彻底比较素食和地中海饮食,并就其对健康的影响得出更确切的结论。
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引用次数: 1
Functional Properties and Bioactivities of Protein Powder Prepared from Skipjack Tuna (Katsuwonus pelamis) Liver Using the pH Shift Process pH漂移法制备鲣鱼肝脏蛋白粉的功能特性及生物活性研究
IF 2.4 4区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-11-14 DOI: 10.31883/pjfns/155225
Chanita Jeerakul, Lalitphan Kitsanayanyong, J. Mookdasanit, W. Klaypradit, P. Tepwong
Skipjack tuna pelamis liver contains high-quality proteins which can potentially serve as an excellent source of functional pro - tein ingredients. Thus, this study was conceptualized to evaluate the physicochemical, functional, and biological properties of proteins from TL using the pH shift process. The pH shift process was conducted through solubilization of TL at pH from 1.5 to 12.5, and the solubilized proteins at pH 2.5, 3.5, 10.5 and 11.5 were precipitated at pH 5.5. Finally, the tuna liver protein powders after the processes at pH 2.5 and 11.5 (TLP 2.5 and TLP 11.5, respectively) were obtained by freeze-drying, i.e. those with the highest extraction and protein recovery yields under acidic and alkaline conditions. Protein contents of TLPs were higher and lower, respectively, compared to the TL powder (control). Glutamic acid, aspartic acid, and alanine were prominent amino acids found in both TLPs. Foaming properties and water/oil holding capacity were higher in TLP 11.5, while protein solubility and emulsion properties were greater in TLP 2.5 compared between groups. Additionally, the DPPH • and ABTS •+ scavenging activities, as well as the angiotensin I-converting enzyme inhibitory activity, were remarkably higher in TLP 11.5 than in TLP 2.5. On the other hand, significant ferrous-ion chelating activity was observed in TLP 2.5. In conclusion, TLP 11.5 could serve as an alternative functional protein ingredient that provides essential amino acids, functional properties, and bioactivities. TLP 11.5 – Tuna liver protein powder from solubili zation at pH 11.5; WHC – Water holding capacity.
鲣鱼的肝脏含有高质量的蛋白质,可以作为功能性蛋白质成分的极好来源。因此,本研究的概念是利用pH转移过程来评估TL中蛋白质的物理化学,功能和生物学特性。在pH从1.5到12.5的条件下,通过TL的增溶进行pH移位过程,在pH为5.5的条件下沉淀出pH为2.5、3.5、10.5和11.5的溶解蛋白。最后,在pH为2.5和11.5 (TLP分别为2.5和11.5)条件下冷冻干燥得到金枪鱼肝蛋白粉,即在酸性和碱性条件下提取率和蛋白质回收率最高的蛋白粉。与TL粉(对照)相比,TLPs的蛋白质含量分别较高和较低。谷氨酸、天冬氨酸和丙氨酸是在两个TLPs中发现的主要氨基酸。与组间比较,TLP 11.5组的泡沫性能和水/油容量更高,而TLP 2.5组的蛋白质溶解度和乳液性能更高。此外,TLP 11.5对DPPH•和ABTS•+的清除活性以及血管紧张素i转换酶的抑制活性显著高于TLP 2.5。另一方面,tlp2.5具有显著的铁离子螯合活性。综上所述,TLP 11.5可以作为一种替代的功能性蛋白成分,提供必需氨基酸、功能特性和生物活性。tlp11.5 -金枪鱼肝蛋白粉,pH值为11.5;WHC -持水量。
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引用次数: 0
β-Glucan and Aronia (Aronia melanocarpa) Phenolics: Interactions During In Vitro Simulated Gastrointestinal Digestion and Adsorption β-葡聚糖和野樱莓酚类物质:体外模拟胃肠道消化和吸附过程中的相互作用
IF 2.4 4区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-11-14 DOI: 10.31883/pjfns/155281
L. Jakobek, Jozo Ištuk, I. Tomac, P. Matić
Interactions between phenolics and dietary fibers, such as β -glucan, are important for the bioactivities of phenolics. However, interactions between aronia phenolics and β -glucan in the digestion process, both promoted for their health benefits, have not been studied. The aim was to study the inter actions between aronia phenolics and β -glucan in an in vitro simulated digestion and in the adsorption process. After extracting aronia phenolics with chemically- and enzymatically-assisted extraction, and characterizing the phenolics, the aronia extract was subjected to simulated oral, gastric, and in testinal digestion without or with β -glucan. Flavonol release increased throughout oral, gastric, and intestinal digestion, while the recovery of phenolic acids and anthocyanins after an increase in the gastric phase, decreased in the intestinal phase. β -Glucan entrapped phenolics, lowering the quantities of recovered phenolics. It also adsorbed aronia phenolics at pH 1.5, 3.0, and 7.0. In comparison to 15 and 30 mg/L concentrations of β -glucan, a solu - tion with the lower concentration (15 mg/L) allowed for the entrapment of the higher quantity of phenolics and had high adsorption capacity. Entrap ment of aronia phenolics by β -glucan is important for the bioaccessibility and concentration of phenolics that reach the lower parts of the digestive tract which depends on β -glucan concentration.
酚类物质与膳食纤维(如β -葡聚糖)之间的相互作用对酚类物质的生物活性至关重要。然而,在消化过程中,野樱草酚类物质和β -葡聚糖之间的相互作用,都因其健康益处而得到促进,但尚未研究。目的是研究野樱草酚类物质与β -葡聚糖在体外模拟消化和吸附过程中的相互作用。在化学和酶辅助提取野樱草酚类物质,并对酚类物质进行表征后,对野樱草提取物进行无β -葡聚糖或有β -葡聚糖的模拟口服、胃和肠消化。黄酮醇的释放在整个口服、胃和肠消化过程中增加,而酚酸和花青素的回收在胃期增加后在肠期减少。β -葡聚糖包裹了酚类物质,降低了酚类物质的回收率。在pH值为1.5、3.0和7.0时,它也能吸附野樱草酚类物质。与浓度为15和30 mg/L的β -葡聚糖相比,浓度较低的溶液(15 mg/L)可以吸附较多的酚类物质,并且具有较高的吸附能力。β -葡聚糖捕获野野葡萄酚类物质对到达消化道下部的酚类物质的生物可及性和浓度至关重要,这取决于β -葡聚糖的浓度。
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引用次数: 0
Physicochemical and Sensory Properties with Special Emphasis on Thermal Characteristics of Whey Butter from Gouda Cheese Production Compared to Milk Butter 高达干酪乳清黄油与乳黄油的理化和感官特性——特别强调热特性
IF 2.4 4区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-11-14 DOI: 10.31883/pjfns/155838
Oskar Brożek, K. Kiełczewska, K. Bohdziewicz
* The aim of this study was to characterise milk fat from whey butter and to identify potential differences between whey butter (WB) and sweet cream butter (milk butter – MB). The fatty acid (FA) profile, thermal properties, colour parameters, texture properties, and sensory attributes of MB and WB were compared. The values of texture properties (firmness, brittleness, and cohesiveness) and colour parameters (values of b * and the yellowness index) of WB were lower than MB. The sensory analysis showed lower values of consistency descriptors (firmness, brittleness, cohesiveness), a less intense nutty and milky aroma, and a more intense cheesy aroma and taste in WB than in MB. WB was more abundant in monounsaturated, polyunsaturated, and long-chain FAs, including C18:0, C18:1 Σ t , C18:1 Σ c , C18:2, C18:3, and C18:2 c 9, t 11, and it was less abundant in saturated and medium-chain FAs, including C10:0, C12:0, C14:0, C14:1, C15:0, C16:0, and C16:1, relative to MB. Water content (MB vs WB and the corresponding fats) and thermal history (single vs repeated heating and cooling treatments) affected differential scanning calorimetry curves and phase transition peaks. The principal component analysis revealed that the FA profile influenced the crystallisation and melting peaks of MB fat (MBF) and WB fat (WBF). WBF crystallisation occurred at a lower temperature, was characterised by lower enthalpy, and proceeded more rapidly than MBF crystallisation. Vari ous fat fractions had different melting characteristics, and most WBF fractions were characterised by lower melting enthalpy and a smaller maximum difference in heat flow than MBF. Whey butter and milk butter differed in physicochemical properties and sensory attributes, and their thermal profiles depended on the FA profile, water content, and thermal history.
*本研究的目的是表征乳清黄油中的乳脂,并确定乳清黄油(WB)和甜奶油黄油(牛奶黄油-MB)之间的潜在差异。比较了MB和WB的脂肪酸(FA)特性、热性能、颜色参数、质地特性和感官特性。WB的质地特性(硬度、脆性和内聚性)和颜色参数(b*值和黄度指数)的值低于MB。感官分析显示,WB中的稠度描述符(硬度、脆性、内聚性)值较低,坚果和乳白色香气较低,奶酪香气和味道较浓。相对于MB,WB在单不饱和、多不饱和和长链FA中更丰富,包括C18:0、C18:1∑t、C18:1σc、C18:2、C18:3和C18:2c9、t11,而在饱和和中链FA中含量较低,包括C10:0、C12:0、C14:0、C14:1、C15:0、C16:0和C16:1。水含量(MB与WB和相应的脂肪)和热历史(单一与重复加热和冷却处理)影响差示扫描量热曲线和相变峰。主成分分析表明,FA分布影响MB脂肪(MBF)和WB脂肪(WBF)的结晶和熔融峰。WBF结晶发生在较低的温度下,其特征是焓较低,并且比MBF结晶进行得更快。不同的脂肪组分具有不同的熔融特性,并且大多数WBF组分的特征是比MBF具有更低的熔融焓和更小的最大热流差。乳清黄油和牛奶黄油在物理化学性质和感官属性上不同,它们的热特性取决于FA特性、含水量和热历史。
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引用次数: 1
Effect of the Addition of Edible Insect Flour from Yellow Mealworm (Tenebrio molitor) on the Sensory Acceptance, and the Physicochemical and Textural Properties of Sponge Cake 添加黄粉虫食虫粉对海绵蛋糕感官接受度、理化性质及质地的影响
IF 2.4 4区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-11-14 DOI: 10.31883/pjfns/155405
S. Kowalski, A. Mikulec, M. Skotnicka, B. Mickowska, M. Makarewicz, R. Sabat, Anna Wywrocka-Gurgul, Aleksandra Mazurek
The aim of this study was to evaluate the possibility of using insect flour for sponge cake supplementation. Consumer acceptance, chemical com - position, textural properties, and microbiological characteristics were determined. The addition of mealworm flour significantly increased the content of nutrients, especially protein, ash, lipid, and dietary fiber. Mealworm flour influenced the color parameters as well as textural properties. The fatty acid profile was dominated by monounsaturated fatty acids, ranging from 9.72 g/100 g for wheat flour sponge cake to 41.82 g/100 g for sponge cake with 20% addition of mealworm flour. The amino acid profile of mealworm flour was characterized by a significantly higher content of essential amino acids compared to wheat flour and sponge cakes. Sponge cake supplementation resulted in a good nutritional value of protein except for lysine which was the limiting amino acid in all samples. However, the limiting amino acid index was 63.04-63.10% compared to 30.38% for the mealworm and wheat sponge cake, respectively. The presence of insect flour reduced the organoleptic properties of the obtained sponge cakes, regardless of its quantity. The addition of mealworm flour contributed to a significant reduction in the hardness and fracturability of the sponge cakes on the baking day and dur - ing the 30-day storage. Insect flour addition did not reduce the microbiological safety of the final product. The study results indicate the possibility of using mealworm flour in the production of confectionery products.
本研究的目的是评估使用昆虫粉补充海绵蛋糕的可能性。测定了消费者接受度、化学成分、质地特性和微生物特性。粉虫粉的添加显著增加了营养成分,尤其是蛋白质、灰分、脂质和膳食纤维的含量。肉糜粉影响了颜色参数以及质地特性。脂肪酸分布以单不饱和脂肪酸为主,小麦粉海绵蛋糕为9.72 g/100 g,粉虫粉添加量为20%的海绵蛋糕为41.82 g/100 g。与小麦粉和海绵蛋糕相比,粉虫粉的氨基酸特征是必需氨基酸含量显著较高。除赖氨酸是所有样品中的限制性氨基酸外,补充海绵蛋糕的蛋白质具有良好的营养价值。然而,与粉虫和小麦海绵蛋糕的30.38%相比,限制氨基酸指数分别为63.04-63.10%。昆虫粉的存在降低了所获得的海绵蛋糕的感官特性,无论其数量如何。粉虫面粉的添加有助于在烘焙当天和储存30天期间显著降低海绵蛋糕的硬度和可断裂性。昆虫粉的添加并没有降低最终产品的微生物安全性。研究结果表明,粉虫面粉有可能用于糖果产品的生产。
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引用次数: 6
Hydration Kinetics of Nixtamalized White Bitter Lupin (Lupinus albus L.) Seeds 烟化白苦卢平(Lupinus albus L.)水化动力学种子
IF 2.4 4区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-11-14 DOI: 10.31883/pjfns/155362
Hizia Berrou, M. Saleh, K. Al-Ismail
Nixtamalization is usually performed on grains by cooking in an alkaline solution to improve the final product characteristics. White bitter lupin ( Lupinus albus ) seeds were nixtamalized at various concentrations of calcium hydroxide in the range of 0.16–3.33% at 50, 70, and 90°C for 35 min and steeped for 0, 8, 16, and 24 h, and the moisture uptake was determined to model seed hydration kinetics. Moisture uptake increased with in - creasing nixtamalization temperature regardless of calcium hydroxide concentration. The Page and Weibull models adequately described white bitter lupin hydration kinetics during nixtamalization. Model parameters K p (Page model) and α (Weibull model) ranged from 80.2 to 410.03 and from 88.21 and 93.96, respectively, for nixtamalization at different calcium hydroxide concentrations, and from 58.55 to 662.88 and from 68.74 and 132.99, respectively, for nixtamalization at different temperatures. The alkaloid content of raw lupin flour was 1.08 g/100 g and it gradually decreased as a result of nixtamalization in increasingly concentrated calcium hydroxide solutions and higher temperatures. The cracks were visible in the microstructure of nixtamalized seed coats. Their number and size increased with the increase of processing temperature, calcium hydroxide concentration, and steep - ing duration. Overall, the presented results may be useful in optimizing the industrial nixtamalization of lupin seeds and increasing the possibility of their use as a valuable food ingredient.
Nixtamalization通常通过在碱性溶液中烹饪对谷物进行,以改善最终产品的特性。将白苦羽扇豆(Lupinus albus)种子在不同浓度的氢氧化钙(0.16–3.33%)下,在50、70和90°C条件下进行35分钟的杀菌处理,并浸泡0、8、16和24小时,测定水分吸收以模拟种子水合动力学。无论氢氧化钙浓度如何,水分吸收量都随着脱硝温度的升高而增加。Page和Weibull模型充分描述了白苦羽扇豆在非均匀化过程中的水合动力学。对于不同氢氧化钙浓度下的镍塔胺化,模型参数Kp(Page模型)和α(Weibull模型)分别为80.2至410.03和88.21和93.96,对于不同温度下的镍塔胺化,分别为58.55至662.88和68.74和132.99。羽扇豆生粉中的生物碱含量为1.08g/100g,并且由于在浓度越来越高的氢氧化钙溶液中和更高的温度下的去碳化作用,生物碱含量逐渐降低。在纳米化种子涂层的微观结构中可以看到裂纹。它们的数量和大小随着加工温度、氢氧化钙浓度和浸泡时间的增加而增加。总的来说,所提出的结果可能有助于优化羽扇豆种子的工业杀菌,并增加其作为有价值的食品成分的可能性。
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引用次数: 1
Antioxidant Capacity of Lentil Flour Hydrolysates Obtained with Pancreatin 胰蛋白酶水解扁豆粉的抗氧化能力
IF 2.4 4区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-11-13 DOI: 10.31883/pjfns/155932
Katarzyna Sulewska, Anna Rybarczyk-Płońska, M. Karamać
Lentil is a valuable protein-rich crop, the health-promoting value of which can be further enhanced by its protein hydrolysis. In the present study, lentil flour was treated with pancreatin and the antioxidant capacity of the obtained hydrolysates with different degrees of hydrolysis (DH, 4–20%) was determined and compared with that of flour. The molecular weight (MW) distribution of the lentil proteins and their products of hydrolysis was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Size exclusion-high performance liquid chromatography (SE--HPLC) was deployed to show the profile of low MW compounds of the hydrolysates. Additionally, total phenolic contents were determined with the Folin-Ciocalteu’s phenol reagent. The hydrolysates had higher antiradical activity against DPPH • , Trolox equivalent antioxidant capacity (TEAC) measured by the assay with ABTS •+ , ferric reducing antioxidant power (FRAP), and ability to bind Fe 2+ compared to lentil flour. Between the hydro - lysates, the highest DPPH • scavenging activity (EC 50 of 0.298 mg/mL), TEAC (98.6 µmol Trolox/g), FRAP (109.2 µmol Fe 2+ /g), antioxidant capacity of lipid-soluble compounds (ACL) determined by photoluminescence method (4.32 µmol Trolox/g), and Fe 2+ chelating activity (80% at hydrolysate concentration of 0.3 mg/mL) were found for those with low DH (4–8%), which contained some subunits of proteins, polypeptides, and peptides with a wide MW range (0.556–66.0 kDa). The total phenolic content increased with increasing DH. In conclusion, the antioxidant capacity of lentil flour can be significantly improved by the limited hydrolysis of its proteins with pancreatin, as a result of the release of polypeptides and peptides with a wide range of MW. Thus modified lentil flour may be addressed and explored in future research as a functional food ingredient.
小扁豆是一种珍贵的富含蛋白质的作物,其蛋白质水解可进一步提高其保健价值。本研究用胰酶对扁豆粉进行处理,测定不同水解程度(DH, 4-20%)的水解产物的抗氧化能力,并与面粉进行比较。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析了小扁豆蛋白及其水解产物的分子量分布。采用粒径排除-高效液相色谱(SE- HPLC)分析了水解产物的低分子量化合物。另外,用Folin-Ciocalteu酚试剂测定总酚含量。与扁豆粉相比,其水解产物具有更高的抗DPPH•自由基活性、ABTS•+测定的Trolox当量抗氧化能力(TEAC)、铁还原抗氧化能力(FRAP)和结合fe2 +的能力。在水解产物中,低DH(4-8%)的DPPH•清除活性最高(ec50为0.298 mg/mL), TEAC(98.6µmol Trolox/g), FRAP(109.2µmol Fe 2+ /g),光致发光法测定的脂溶性化合物(ACL)的抗氧化能力(4.32µmol Trolox/g),铁2+螯合活性(在0.3 mg/mL的水解产物浓度下,铁2+螯合活性为80%),其中含有一些亚基的蛋白质,多肽和宽分子量范围的肽(0.356 - 66.0 kDa)。总酚含量随DH的增加而增加。综上所述,小扁豆粉的抗氧化能力可以通过胰蛋白酶的有限水解而显著提高,释放出多肽和分子量范围较宽的肽。因此,改性扁豆粉作为一种功能性食品原料,在今后的研究中有一定的应用前景。
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引用次数: 1
Jamun Seed: A Review on Bioactive Constituents, Nutritional Value and Health Benefits Jamun种子:生物活性成分、营养价值和健康益处综述
IF 2.4 4区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-09-05 DOI: 10.31883/pjfns/152568
Yamini Tak, Manpreet Kaur, M. Jain, M. Samota, N. Meena, Gurpreet Kaur, Rajendra Kumar, D. Sharma, J. Lorenzo, R. Amarowicz
Jamun fruit, a member of the Myrtaceae family, is commercially grown in tropical and subtropical areas of the world for its fruits with sweet, sour, and astringent luscious flesh. Seeds of jamun fruits are discarded as trash during the industrial processing of fruit pulp into beverages, jellies, jam, vinegar, wine, and squash. These seeds are a potential source of bioactive compounds including hydrolysable tannins, phenolic acids, flavonoids, other phenolics, terpenoids, phloroglucinol derivatives and saponins, which have been endorsed several biological activities, such as antidiabetic, anticancer, anti-inflammatory, antioxidant, antimicrobial, antihyperlipidemic and antihypercholesterolemic, as well as cardioprotective, hepatoprotective and neu - roprotective properties. High contents of carbohydrates, dietary fiber, minerals, and ascorbic acid have also been found in jamun seeds. However, potential utilization of these seeds as innovative implements for health benefits has not yet been fully understood. We aim to compile scientific research and recent advances on jamun seed nutritional profile, bioactive compounds composition, bioactive properties, and their potential as an ingredient in functional food formulation.
Jamun果是杨梅科的一员,因其果肉甜、酸、涩、甘美而在世界热带和亚热带地区商业种植。在将果肉工业加工成饮料、果冻、果酱、醋、葡萄酒和南瓜的过程中,果酱的种子被当作垃圾丢弃。这些种子是生物活性化合物的潜在来源,包括可水解单宁、酚酸、黄酮类化合物、其他酚类化合物、萜类化合物、间苯三酚衍生物和皂苷,这些化合物已被认可具有多种生物活性,如抗糖尿病、抗癌、抗炎、抗氧化、抗菌、抗高血压和抗高胆固醇血症,以及心脏保护、肝保护和神经保护特性。在果酱种子中也发现了高含量的碳水化合物、膳食纤维、矿物质和抗坏血酸。然而,这些种子作为有益健康的创新工具的潜在用途尚未得到充分了解。我们的目的是汇编关于jamun种子营养概况、生物活性化合物组成、生物活性特性及其作为功能性食品配方成分的潜力的科学研究和最新进展。
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引用次数: 7
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Polish Journal of Food and Nutrition Sciences
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