Polish medical sciences and history bulletin最新文献

A dose of 0.0015 mug of Actinomycin D per 1 ml, applied for 4-5 days has some inhibitory effect on RNA and protein synthesis, leading to a diminished proliferation of WISH cells. This dose does not exert a destructive effect on cell morphology. Two to four times higher doses, applied for the same time were toxic. Morphological changes observed at various times of exposure of cells to different concentrations of drug as well as changes in cell proliferation are described. The attempts to select Actinomycin D-resistant cells have failed.

The paper describes the biological activity and pharmacological properties, particularly the spasmolytic and analgesis effects, of newly synthesized 4-propionyl-4-3(3-chlorphenyl)-1-[3-(4-methyl-piperazine)-propyl]-piperidine trihydrochloride (denoted S10).

Som pharmalogical properties of a new, biologically active combpound 4-propionyl-4-(3-chlorphenyl)-1-(3'-dimethylaminoprophy)-piperidine dihydrochloride (symbol S6) are presented.

In 40 patients with hepatocellular and obstructive jaundice and in 20 healthy subjects the pattern of serum leucine arylamidase (LAP) isoenzymes was determined. Polyacrylamide gel electrophoresis was used for demonstration of enzyme non-homogeneity. It was found that the main enzyme activity in healthy subjects was connected with postalbumins. In some healthy subjects and additional zone of activity was observed in beta lipoproteins. Only exceptionally 3- and 4-fraction enzymograms were found in healthy subjects. Additional activity zones were present in these subjects in the alpha2 macroglobulin region or in the region of slowly migrating haptoglobins. In hepatocellular jaundice, i.e. in viral hepatitis, the total serum LAP activity increased slightly. In the enzymogram this increase was localized in the postalbumin fraction and caused also appearance of additional, sporadically occurring isoenzymes migrating with rapidly moving haptoglobins. In obstructive jaundice of benign or malignant etiology the total serum enzyme activity rose strikingly. In most patients a 6-fraction enzymogram was found. The activity of postalbumin isoenzyme was particularly increased, while no changes were observed in the remaining activity zones sporadically occurring in healthy subjects and in hepatocellular jaundice.

In 69 patients with ischemic heart disease the left ventricular functional state, especially contraction disturbances, was assessed by means of direct methods and polycardiographic measurements. The patients were divided into groups depending on the number of vessels affected by atheromatosis. In direct measurements the following values were determined: the rate of rise of intraventricular pressure--dP/dt max., the interval t-dP/dt max, the index (see article). In polycardiographic determinations the isovolumetric contraction time (ICI), the pre-ejection period (PEP) and the coefficient consisting of PEP in the numerator and ejection time (ET) in the denominator (PEP/ET) were calculated. The degree of changes in direct and polycardiographic parameters depended on the number of vessels affected by the atherosclerotic process. The index (see article) was most sensitive to changes in the contractility. The correlation was statistically significant for all parameters compared in the group of patients with lesions in three coronary arteries and myocardial infarction. In the remaining groups the correlations differed and a statistically significant correlation was demonstrated between particular parameters. The correlation was best between direct measurements and ICT and the PEP/ET index.

In 22 patients with chronic cor pulmonale syndrome plasma renin activity (PRA) was determined at rest and after stimulation by means of intravenous injection of furosemide. In 50% of cases PRA was raised exceeding 250 ng/100 ml/3 hours. The systemic arterial pressure in these patients was normal. The degree of PRA increase after stimulation was lower than in healthy subjects.

The dynamics of regeneration of crushed skeletal muscles in rabbits and rats were studied. In both species the course of regeneration was similar, leading to partial reconstruction of muscle tissue. Experiments on the influence of leucine on regeneration of skeletal muscles in white rats showed that this amino acid stimulates and shortens the process of regeneration.

The effect of acute experimentally induced renal failure after intramuscular injection of glycerol on serum and urine GGTP, LAP and AP activities was studied in 30 rabbits. High doses of glycerol caused shock, myolysis and hemolysis, leading to acute renal insufficiency. Serum urea and creatinine levels significantly increased, there was proteinuria, and significant decrease in 24-hr diuresis, glomerular filtration, and urinary urea excretion. The changes in LAP and AP activities were significant, and in GGTP-nonsignificant. In the urine GGTP and LAP increased significantly, and AP nonsignificantly. Urinary excretion of AP increased significantly, and GGTP and LAP nonsignificantly. The highest activity and urinary excretion of GGTP and LAP were observed on the 2nd day, and of AP--on the 5th day of renal failure.

The outflow from lymphatic vessels running along with the right cardinal bronchus was interrupted in six dogs by the removal of the corresponding hilar lymph nodes. After 6-8 days the right lung showed slight subpleural areas of atelectasis and edema. Edema of endothelial cells of the lung septal capilaries was observed electron-microscopically. This lesion is similar to the findings in cases of mitral stenosis.

Peripheral blood lymphocytes were investigated in 20 patients with acute viral hepatitis. For assessment of B lymphocytes the rosette test was carried out using sheep erythrocytes coated with antibodies and murine complement. T lymphocytes were recognized by their ability of binding uncoated sheep erythrocytes. In 16 cases the results of EAC test in patients with viral hepatitis resembled those in healthy donors. In 3 cases a significant decrease was observed and in one case a significant rise was found in the number of cells forming EAC rosettes. The T lymphocyte count was within normal range in 19 investigated cases. The results suggest that impaired T lymphocyte reactivity to PHA in acute viral hepatitis is due to a disturbed function of T lymphocytes and not to a decrease in their number. In cases associated with significant hepatocellular damage a reduction in the number of circulating B lymphocytes was observed.