Maria Carolina Rodella Manzano, Daniel Angelo Felippi, Larissa Sayuri Moreira Sugai, Ricardo Sawaya, Maria Luisa da Silva, Gabriela Cabral Rezende
Biodiversity conservation faces challenges due to a lack of accurate information on species occurrence. Various techniques have been used to survey species diversity and estimate population density, but monitoring species over large spatial and temporal scales remains challenging. Passive acoustic monitoring (PAM) has emerged as a cost-effective and non-invasive method for monitoring biodiversity. PAM utilizes autonomous recording units (ARUs) installed in different areas and is particularly relevant for monitoring threatened species in tropical forest regions. In the case of non-human primates, PAM has proven effective in detecting endangered species, monitoring populations, studying vocal behavior, and evaluating territory use. The black lion tamarin (Leontopithecus chrysopygus), an endangered species of the Atlantic Forest, relies on acoustic signals for communication. This study proposes a PAM protocol for monitoring arboreal primates using the black lion tamarin as a model. It reviews PAM's use in primate research and emphasizes defining target vocalizations. We recommend optimal recording conditions, including distance, height, and equipment. Recordings should be positioned high above the ground, considering the arboreal nature of primates. The choice of spatial distribution, including random placement, transects, and grids, depends on the research question and objectives. Lastly, the study addresses the recording schedule, considering periods of greater species activity, such as from sunrise to sunset. In summary, this study highlights PAM's potential for monitoring arboreal primates providing recommendations for vocalizations, recording conditions, equipment, spatial distribution, and schedules, contributing to effective monitoring, and supporting conservation efforts in tropical forests.
{"title":"Calling for the future of conservation: a protocol for passive acoustic monitoring of small arboreal primates","authors":"Maria Carolina Rodella Manzano, Daniel Angelo Felippi, Larissa Sayuri Moreira Sugai, Ricardo Sawaya, Maria Luisa da Silva, Gabriela Cabral Rezende","doi":"10.32673/bjm.vie92.122","DOIUrl":"https://doi.org/10.32673/bjm.vie92.122","url":null,"abstract":"Biodiversity conservation faces challenges due to a lack of accurate information on species occurrence. Various techniques have been used to survey species diversity and estimate population density, but monitoring species over large spatial and temporal scales remains challenging. Passive acoustic monitoring (PAM) has emerged as a cost-effective and non-invasive method for monitoring biodiversity. PAM utilizes autonomous recording units (ARUs) installed in different areas and is particularly relevant for monitoring threatened species in tropical forest regions. In the case of non-human primates, PAM has proven effective in detecting endangered species, monitoring populations, studying vocal behavior, and evaluating territory use. The black lion tamarin (Leontopithecus chrysopygus), an endangered species of the Atlantic Forest, relies on acoustic signals for communication. This study proposes a PAM protocol for monitoring arboreal primates using the black lion tamarin as a model. It reviews PAM's use in primate research and emphasizes defining target vocalizations. We recommend optimal recording conditions, including distance, height, and equipment. Recordings should be positioned high above the ground, considering the arboreal nature of primates. The choice of spatial distribution, including random placement, transects, and grids, depends on the research question and objectives. Lastly, the study addresses the recording schedule, considering periods of greater species activity, such as from sunrise to sunset. In summary, this study highlights PAM's potential for monitoring arboreal primates providing recommendations for vocalizations, recording conditions, equipment, spatial distribution, and schedules, contributing to effective monitoring, and supporting conservation efforts in tropical forests.","PeriodicalId":204477,"journal":{"name":"Brazilian Journal of Mammalogy","volume":"67 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139853596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maria Carolina Rodella Manzano, Daniel Angelo Felippi, Larissa Sayuri Moreira Sugai, Ricardo Sawaya, Maria Luisa da Silva, Gabriela Cabral Rezende
Biodiversity conservation faces challenges due to a lack of accurate information on species occurrence. Various techniques have been used to survey species diversity and estimate population density, but monitoring species over large spatial and temporal scales remains challenging. Passive acoustic monitoring (PAM) has emerged as a cost-effective and non-invasive method for monitoring biodiversity. PAM utilizes autonomous recording units (ARUs) installed in different areas and is particularly relevant for monitoring threatened species in tropical forest regions. In the case of non-human primates, PAM has proven effective in detecting endangered species, monitoring populations, studying vocal behavior, and evaluating territory use. The black lion tamarin (Leontopithecus chrysopygus), an endangered species of the Atlantic Forest, relies on acoustic signals for communication. This study proposes a PAM protocol for monitoring arboreal primates using the black lion tamarin as a model. It reviews PAM's use in primate research and emphasizes defining target vocalizations. We recommend optimal recording conditions, including distance, height, and equipment. Recordings should be positioned high above the ground, considering the arboreal nature of primates. The choice of spatial distribution, including random placement, transects, and grids, depends on the research question and objectives. Lastly, the study addresses the recording schedule, considering periods of greater species activity, such as from sunrise to sunset. In summary, this study highlights PAM's potential for monitoring arboreal primates providing recommendations for vocalizations, recording conditions, equipment, spatial distribution, and schedules, contributing to effective monitoring, and supporting conservation efforts in tropical forests.
{"title":"Calling for the future of conservation: a protocol for passive acoustic monitoring of small arboreal primates","authors":"Maria Carolina Rodella Manzano, Daniel Angelo Felippi, Larissa Sayuri Moreira Sugai, Ricardo Sawaya, Maria Luisa da Silva, Gabriela Cabral Rezende","doi":"10.32673/bjm.vie92.122","DOIUrl":"https://doi.org/10.32673/bjm.vie92.122","url":null,"abstract":"Biodiversity conservation faces challenges due to a lack of accurate information on species occurrence. Various techniques have been used to survey species diversity and estimate population density, but monitoring species over large spatial and temporal scales remains challenging. Passive acoustic monitoring (PAM) has emerged as a cost-effective and non-invasive method for monitoring biodiversity. PAM utilizes autonomous recording units (ARUs) installed in different areas and is particularly relevant for monitoring threatened species in tropical forest regions. In the case of non-human primates, PAM has proven effective in detecting endangered species, monitoring populations, studying vocal behavior, and evaluating territory use. The black lion tamarin (Leontopithecus chrysopygus), an endangered species of the Atlantic Forest, relies on acoustic signals for communication. This study proposes a PAM protocol for monitoring arboreal primates using the black lion tamarin as a model. It reviews PAM's use in primate research and emphasizes defining target vocalizations. We recommend optimal recording conditions, including distance, height, and equipment. Recordings should be positioned high above the ground, considering the arboreal nature of primates. The choice of spatial distribution, including random placement, transects, and grids, depends on the research question and objectives. Lastly, the study addresses the recording schedule, considering periods of greater species activity, such as from sunrise to sunset. In summary, this study highlights PAM's potential for monitoring arboreal primates providing recommendations for vocalizations, recording conditions, equipment, spatial distribution, and schedules, contributing to effective monitoring, and supporting conservation efforts in tropical forests.","PeriodicalId":204477,"journal":{"name":"Brazilian Journal of Mammalogy","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139793840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Records of collecting nest building material lack descriptions or are missing for most didelphids. We describe the tail use in this behavior in Metachirus myosuros on Atlantic Rainforest and compare it with similar behaviors reported for other didelphids. The behavior described here is like those reported for other closely related species, and likely can be traced at least to the last common ancestor of Didelphini and Metachirini (~15 Maa). However, scarcity of data on other taxa makes it impossible to state that this behavior was already present in the start of the Didelphidae clade.
{"title":"Record of nesting material collection behavior in Metachirus myosuros (Didelphimorphia: Didelphidae)","authors":"Bernardo Faria Sartori, William Corrêa Tavares","doi":"10.32673/bjm.vi92.92","DOIUrl":"https://doi.org/10.32673/bjm.vi92.92","url":null,"abstract":"Records of collecting nest building material lack descriptions or are missing for most didelphids. We describe the tail use in this behavior in Metachirus myosuros on Atlantic Rainforest and compare it with similar behaviors reported for other didelphids. The behavior described here is like those reported for other closely related species, and likely can be traced at least to the last common ancestor of Didelphini and Metachirini (~15 Maa). However, scarcity of data on other taxa makes it impossible to state that this behavior was already present in the start of the Didelphidae clade.","PeriodicalId":204477,"journal":{"name":"Brazilian Journal of Mammalogy","volume":"45 26","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139603360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Camera traps are widely employed for studying terrestrial mammals worldwide. Over the past two decades (2003-2023), they have undergone numerous advancements and refinements, making their application possible for the most diverse study types. In this review, we aim to present a historical background, applications, and future perspectives on the use of camera traps in the research on terrestrial mammals in Brazil. We analyzed 339 peer-reviewed studies published between 2003 and 2023. The initial sampling years of these studies range from 1998 to 2020, with the majority (287 studies) concentrated from 2005 onwards. The studies were primarily focused on the southeast (132) and midwest (78) regions of Brazil - areas that historically receive more financial support and research assistance. The most featured biome was the Atlantic Forest (164), while the Caatinga and Pampa exhibited the lowest number of studies (30 and 8, respectively). Most of the studies were focused on aspects of species composition and richness (93), occupancy and habitat use (70), and activity patterns (65). Considering the knowledge gaps on mammal occurrence and distribution in North and Northeast Brazil, we suggest directing research investments toward these regions, as well as for historically neglected biomes, such as Caatinga and Pampa.
{"title":"Use of camera traps in the research on terrestrial mammals in Brazil: history, methodological applications, and perspectives","authors":"Maria Eduarda Soares Alberti, Barbara Lima-Silva","doi":"10.32673/bjm.vie92.108","DOIUrl":"https://doi.org/10.32673/bjm.vie92.108","url":null,"abstract":"Camera traps are widely employed for studying terrestrial mammals worldwide. Over the past two decades (2003-2023), they have undergone numerous advancements and refinements, making their application possible for the most diverse study types. In this review, we aim to present a historical background, applications, and future perspectives on the use of camera traps in the research on terrestrial mammals in Brazil. We analyzed 339 peer-reviewed studies published between 2003 and 2023. The initial sampling years of these studies range from 1998 to 2020, with the majority (287 studies) concentrated from 2005 onwards. The studies were primarily focused on the southeast (132) and midwest (78) regions of Brazil - areas that historically receive more financial support and research assistance. The most featured biome was the Atlantic Forest (164), while the Caatinga and Pampa exhibited the lowest number of studies (30 and 8, respectively). Most of the studies were focused on aspects of species composition and richness (93), occupancy and habitat use (70), and activity patterns (65). Considering the knowledge gaps on mammal occurrence and distribution in North and Northeast Brazil, we suggest directing research investments toward these regions, as well as for historically neglected biomes, such as Caatinga and Pampa.","PeriodicalId":204477,"journal":{"name":"Brazilian Journal of Mammalogy","volume":"29 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139530725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Many species of opossum (Didelphimorphia) are known to fluoresce, but the significance of this characteristic is unclear. In the interests of contributing to the faunal inventory of fluorescence in Didelphids, we examined 62 specimens of 10 species of Paraguayan opossums under a UV light and describe the patterns observed. Of particular interest is a clear apparent difference in fluorescence between two cryptic and occasionally sympatric species, Marmosa rapposa and Marmosa paraguayana which may be a potential isolating mechanism. Furthermore, we suggest the possibility that fluorescence declines with time since collection in Didelphis albiventris, and the fluorescence in that species is not related to age or sex. While the significance of fluorescence in Didelphids remains obscure, patterns observed show some degree of species specificity within the geographic boundaries of this study.
{"title":"Patterns of bioluminescence in a collection of Paraguayan Didelphidae","authors":"Paul Smith, George Hicks","doi":"10.32673/bjm.vi92.89","DOIUrl":"https://doi.org/10.32673/bjm.vi92.89","url":null,"abstract":"Many species of opossum (Didelphimorphia) are known to fluoresce, but the significance of this characteristic is unclear. In the interests of contributing to the faunal inventory of fluorescence in Didelphids, we examined 62 specimens of 10 species of Paraguayan opossums under a UV light and describe the patterns observed. Of particular interest is a clear apparent difference in fluorescence between two cryptic and occasionally sympatric species, Marmosa rapposa and Marmosa paraguayana which may be a potential isolating mechanism. Furthermore, we suggest the possibility that fluorescence declines with time since collection in Didelphis albiventris, and the fluorescence in that species is not related to age or sex. While the significance of fluorescence in Didelphids remains obscure, patterns observed show some degree of species specificity within the geographic boundaries of this study.","PeriodicalId":204477,"journal":{"name":"Brazilian Journal of Mammalogy","volume":"14 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We recorded the first event of six-banded armadillo carrying offspring in their mouth in Brazil on October 2022, at 06:36, Goiás. We searched for additional records in Web of Science, Scopus, Google Scholar, and social media platforms (Instagram, TikTok, YouTube). We found two articles and seven Brazilian videos depicting armadillos carrying offspring in their mouths. While this is a common reproductive behavior, it might also relate to shelter disturbance. Despite incomplete social media records regarding date, time, location, and collector, they underscore the significance of citizen science in showcasing Brazilian biodiversity and contributing to our understanding of armadillos' natural history.
我们于 2022 年 10 月 06:36 在巴西戈亚斯州首次记录到六带犰狳口含后代的事件。我们在 Web of Science、Scopus、Google Scholar 和社交媒体平台（Instagram、TikTok、YouTube）上搜索了更多记录。我们发现两篇文章和七段巴西视频描述了犰狳叼着后代的情景。虽然这是一种常见的繁殖行为，但也可能与庇护所受到干扰有关。尽管社交媒体上关于日期、时间、地点和收集者的记录并不完整，但它们强调了公民科学在展示巴西生物多样性方面的重要性，并有助于我们了解犰狳的自然历史。
{"title":"First published records of six-banded armadillo Euphractus sexcinctus carrying offspring in its mouth in Brazil","authors":"Luana Flores, W. Hannibal","doi":"10.32673/bjm.vi92.128","DOIUrl":"https://doi.org/10.32673/bjm.vi92.128","url":null,"abstract":"We recorded the first event of six-banded armadillo carrying offspring in their mouth in Brazil on October 2022, at 06:36, Goiás. We searched for additional records in Web of Science, Scopus, Google Scholar, and social media platforms (Instagram, TikTok, YouTube). We found two articles and seven Brazilian videos depicting armadillos carrying offspring in their mouths. While this is a common reproductive behavior, it might also relate to shelter disturbance. Despite incomplete social media records regarding date, time, location, and collector, they underscore the significance of citizen science in showcasing Brazilian biodiversity and contributing to our understanding of armadillos' natural history.","PeriodicalId":204477,"journal":{"name":"Brazilian Journal of Mammalogy","volume":"25 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Lourenço, Matheus Pereira e Silva, H. G. Bergallo
Estudos ecológicos relacionados a ectoparasitos não são frequentes, principalmente, quando se trata de amostragens de longa duração. O presente estudo teve como objetivo relatar o protocolo de coleta de ectoparasitos de pequenos mamíferos terrestres não voadores realizada em dois módulos RAPELD na Ilha Grande, município de Angra dos Reis, estado do Rio de Janeiro. Assim, buscamos detalhar as atividades metodológicas sobre as pesquisas com ectoparasitos de pequenos mamíferos, visando facilitar os procedimentos para futuras pesquisas. As campanhas de monitoramento de pequenos mamíferos ocorreram de 2013 a 2019, enquanto o procedimento de coleta de ectoparasitos aqui detalhado abrangeu as campanhas de 2013 a 2018. Dez espécies de pequenos mamíferos foram capturadas em armadilhas de contenção, inseridos em sacos plásticos e triados no local. Os ectoparasitos foram removidos por escovação e catação e armazenados em etanol absoluto. No laboratório, as amostras foram triadas sob microscópio estereoscópico e separados em grupos taxonômicos para posterior determinação da espécie ou para diafanização e montagem em lâminas semipermanentes. O protocolo executado buscou minimizar qualquer tipo de contaminação, que poderiam ter implicações ecológicas, devido a confusões de associações parasito-hospedeiro. Os métodos descriminados no laboratório para clarificação dos espécimes foram eficazes, permitindo a determinação de espécies de diferentes taxa.
{"title":"Protocolo de coleta, armazenamento e preparação de ectoparasitos de pequenos mamíferos terrestres em estudos de monitoramento nos módulos RAPELD Ilha Grande, estado do Rio de Janeiro","authors":"E. Lourenço, Matheus Pereira e Silva, H. G. Bergallo","doi":"10.32673/bjm.vie92.105","DOIUrl":"https://doi.org/10.32673/bjm.vie92.105","url":null,"abstract":"Estudos ecológicos relacionados a ectoparasitos não são frequentes, principalmente, quando se trata de amostragens de longa duração. O presente estudo teve como objetivo relatar o protocolo de coleta de ectoparasitos de pequenos mamíferos terrestres não voadores realizada em dois módulos RAPELD na Ilha Grande, município de Angra dos Reis, estado do Rio de Janeiro. Assim, buscamos detalhar as atividades metodológicas sobre as pesquisas com ectoparasitos de pequenos mamíferos, visando facilitar os procedimentos para futuras pesquisas. As campanhas de monitoramento de pequenos mamíferos ocorreram de 2013 a 2019, enquanto o procedimento de coleta de ectoparasitos aqui detalhado abrangeu as campanhas de 2013 a 2018. Dez espécies de pequenos mamíferos foram capturadas em armadilhas de contenção, inseridos em sacos plásticos e triados no local. Os ectoparasitos foram removidos por escovação e catação e armazenados em etanol absoluto. No laboratório, as amostras foram triadas sob microscópio estereoscópico e separados em grupos taxonômicos para posterior determinação da espécie ou para diafanização e montagem em lâminas semipermanentes. O protocolo executado buscou minimizar qualquer tipo de contaminação, que poderiam ter implicações ecológicas, devido a confusões de associações parasito-hospedeiro. Os métodos descriminados no laboratório para clarificação dos espécimes foram eficazes, permitindo a determinação de espécies de diferentes taxa.","PeriodicalId":204477,"journal":{"name":"Brazilian Journal of Mammalogy","volume":"59 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139449275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Os polimorfismos de nucleotídeo único (SNPs) são marcadores genéticos que podem ser usados em estudos de genômica populacional de mamíferos Neotropicais. Este ensaio é um guia com sugestões e dicas para as principais etapas de um trabalho envolvendo SNPs e genômica populacional a partir de uma técnica de sequenciamento de representação reduzida ou RRS. Neste ensaio é abordado como pensar um delineamento experimental eficiente, desde a escolha das amostras, como preparar as bibliotecas genômicas até dicas para verificar a qualidade do sequenciamento e dos dados. Durante o texto são discutidos os pontos-chaves dos trabalhos com genômica de populações, com indicações de estudos e referências para fundamentar os projetos de pesquisa e o aprendizado. Vale ressaltar que o guia contém sugestões e não regras para realização desse tipo de pesquisa. No fim, é fornecido um banco de dados da espécie de roedor Proechimys steerei da família Echimyidae e uma pipeline para realizar na prática as etapas de filtragem de SNPs e algumas análises estatísticas básicas, usando pacotes de R. Espera-se que esse guia facilite a compreensão de alunos e pesquisadores que estão iniciando no campo da genômica. Mesmo sendo um guia usando exemplos de mamíferos Neotropicais e de RRS, boa parte do que é discutido neste guia poderá ser aplicado a qualquer banco de dados de SNPs gerado a partir de qualquer técnica de sequenciamento genômico e de qualquer grupo de organismo.
单核苷酸多态性（SNP）是可用于新热带哺乳动物种群基因组学研究的遗传标记。这篇文章是一份指南，为使用缩减表征测序（RRS）技术进行涉及单核苷酸多态性和种群基因组学研究的主要阶段提供建议和提示。文章从样本的选择、如何准备基因组文库到检查测序和数据质量的技巧，介绍了如何进行有效的实验设计。文中讨论了群体基因组学的工作要点，并提供了研究和参考资料，以支持研究项目和学习。值得强调的是，指南中包含的是开展此类研究的建议，而不是规则。最后，本指南还提供了一个啮齿类动物（Echimyidae）家族中的 Proechimys steerei 的数据库，以及使用 R 软件包进行 SNP 筛选步骤和一些基本统计分析的实用程序。 希望本指南能有助于初涉基因组学领域的学生和研究人员理解相关知识。虽然本指南以新热带哺乳动物和 RRS 为例，但其中讨论的大部分内容都可应用于任何基因组测序技术和任何生物群体生成的 SNP 数据库。
{"title":"Guia para genômica de populações aplicada a mamíferos Neotropicais","authors":"Jeronymo Dalapicolla","doi":"10.32673/bjm.vie92.120","DOIUrl":"https://doi.org/10.32673/bjm.vie92.120","url":null,"abstract":"Os polimorfismos de nucleotídeo único (SNPs) são marcadores genéticos que podem ser usados em estudos de genômica populacional de mamíferos Neotropicais. Este ensaio é um guia com sugestões e dicas para as principais etapas de um trabalho envolvendo SNPs e genômica populacional a partir de uma técnica de sequenciamento de representação reduzida ou RRS. Neste ensaio é abordado como pensar um delineamento experimental eficiente, desde a escolha das amostras, como preparar as bibliotecas genômicas até dicas para verificar a qualidade do sequenciamento e dos dados. Durante o texto são discutidos os pontos-chaves dos trabalhos com genômica de populações, com indicações de estudos e referências para fundamentar os projetos de pesquisa e o aprendizado. Vale ressaltar que o guia contém sugestões e não regras para realização desse tipo de pesquisa. No fim, é fornecido um banco de dados da espécie de roedor Proechimys steerei da família Echimyidae e uma pipeline para realizar na prática as etapas de filtragem de SNPs e algumas análises estatísticas básicas, usando pacotes de R. Espera-se que esse guia facilite a compreensão de alunos e pesquisadores que estão iniciando no campo da genômica. Mesmo sendo um guia usando exemplos de mamíferos Neotropicais e de RRS, boa parte do que é discutido neste guia poderá ser aplicado a qualquer banco de dados de SNPs gerado a partir de qualquer técnica de sequenciamento genômico e de qualquer grupo de organismo.","PeriodicalId":204477,"journal":{"name":"Brazilian Journal of Mammalogy","volume":"350 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139170140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carlos Rodrigues Moraes Neto, Gabby Neves Guilhon, Leandro O. Salles
As coleções mastozoológicas acondicionam espécimes de forma seca, através da taxidermia, ou úmida, através da preservação em álcool 70%. No caso dos morcegos, ambas preservações são comuns, mas na taxidermia seriada costuma-se manter na pele parte do esqueleto, ficando este material indisponível para análises morfológicas e afins. Neste protocolo, descrevemos uma técnica de taxidermia que possibilita a remoção completa do esqueleto craniano e pós-craniano da pele em poucos passos, podendo ser feita tanto com ambas as asas fechadas ou uma asa aberta e a outra fechada. Dessa forma, é possível analisar ambos os materiais de forma independente.
{"title":"Protocolo técnico de taxidermia shmoo em Chiroptera","authors":"Carlos Rodrigues Moraes Neto, Gabby Neves Guilhon, Leandro O. Salles","doi":"10.32673/bjm.vie92.118","DOIUrl":"https://doi.org/10.32673/bjm.vie92.118","url":null,"abstract":"As coleções mastozoológicas acondicionam espécimes de forma seca, através da taxidermia, ou úmida, através da preservação em álcool 70%. No caso dos morcegos, ambas preservações são comuns, mas na taxidermia seriada costuma-se manter na pele parte do esqueleto, ficando este material indisponível para análises morfológicas e afins. Neste protocolo, descrevemos uma técnica de taxidermia que possibilita a remoção completa do esqueleto craniano e pós-craniano da pele em poucos passos, podendo ser feita tanto com ambas as asas fechadas ou uma asa aberta e a outra fechada. Dessa forma, é possível analisar ambos os materiais de forma independente.","PeriodicalId":204477,"journal":{"name":"Brazilian Journal of Mammalogy","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DIA-BATS","authors":"Leticia Carvalho Farias de Saules","doi":"10.32673/bjm.vi92.97","DOIUrl":"https://doi.org/10.32673/bjm.vi92.97","url":null,"abstract":"","PeriodicalId":204477,"journal":{"name":"Brazilian Journal of Mammalogy","volume":"16 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139260663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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