Pub Date : 2019-01-01DOI: 10.5220/0009587600190028
N. Kurniawan, Dea Jolie Chrestella, Dinda Sherlyndra Hapsari, Fatchiyah
: Naja sputatrix (Javan spitting cobra) is one of medically important snake species in Indonesia which have various dorsal scales color. This research purposes to examine the differences of venom general profile and its phospholipase A 2 (svPLA 2 ) activity from some N. sputatrix with different dorsal scales colors, and to examine the activity of N. sputatrix svPLA 2 in different storage conditions. A total of 6 N. sputatrix from East Java with various dorsal scales color were milked. Venom storage was performed at -80, 4 and 37°C in a maximum period of 14 days. Venom profile and phospholipase A 2 activity were examined through 15% SDS-PAGE and acidimetric method using egg yolk substrate respectively. Statistical analyses were performed to evaluate svPLA 2 activity in every dorsal color and storage condition. Few protein bands range in 16 – 22 kDa are only found in the venom of the certain dorsal color snake. Protein bands at 37°C were found to have the lightest intensity among other groups. The svPLA 2 activity of brown dorsal N. sputatrix is found as the highest activity. An interaction between the storage temperature factor and period factor has effects on post-storage svPLA 2 activity. Storage in 37°C effects on svPLA 2 activity declining compared to the control group and other experimental groups.
{"title":"The Differences of Electrophoretic Profile and Snake Venom Phospholipase A2 (svPLA2) Activity from the Venom of Javan Spitting Cobra, Naja sputatrix, based on Body Scales Color and Storage Condition","authors":"N. Kurniawan, Dea Jolie Chrestella, Dinda Sherlyndra Hapsari, Fatchiyah","doi":"10.5220/0009587600190028","DOIUrl":"https://doi.org/10.5220/0009587600190028","url":null,"abstract":": Naja sputatrix (Javan spitting cobra) is one of medically important snake species in Indonesia which have various dorsal scales color. This research purposes to examine the differences of venom general profile and its phospholipase A 2 (svPLA 2 ) activity from some N. sputatrix with different dorsal scales colors, and to examine the activity of N. sputatrix svPLA 2 in different storage conditions. A total of 6 N. sputatrix from East Java with various dorsal scales color were milked. Venom storage was performed at -80, 4 and 37°C in a maximum period of 14 days. Venom profile and phospholipase A 2 activity were examined through 15% SDS-PAGE and acidimetric method using egg yolk substrate respectively. Statistical analyses were performed to evaluate svPLA 2 activity in every dorsal color and storage condition. Few protein bands range in 16 – 22 kDa are only found in the venom of the certain dorsal color snake. Protein bands at 37°C were found to have the lightest intensity among other groups. The svPLA 2 activity of brown dorsal N. sputatrix is found as the highest activity. An interaction between the storage temperature factor and period factor has effects on post-storage svPLA 2 activity. Storage in 37°C effects on svPLA 2 activity declining compared to the control group and other experimental groups.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75053743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009586800050014
C. A. Kurniasari, Bayu D. Prakoso, Eka Lestari, N. Kurniawan
: One example of a group P-III SVMP is jararhagin which originates from a Bothrops jararaca . This study was conducted to compare the possibility of inhibitors that have the highest effectiveness and exact time of each compound to inhibit hemorragic effect of SVMP. Inhibition of hemorrhagic activity can be done with several types of compounds that have been known to be inhibitors for SVMP especially jararhagin (PDB ID: 1C9G) to bind with integrin 2 1 (PDB ID: 1AOX). There are batimastat (PubChem ID: 5362422) as one of peptidomimetic compounds, EDTA (PubChem ID: 6049) as one of zinc chelating agents, and plant compounds such as hydroxytyrosol (PubChem ID: 82755). The batimastat inhibitory properties from value of binding energy, found that these inhibitor were more easily bound to jararhagin (-289.0 kcal/mol) compared to integrin 2 1 (-277.1 kcal/mol). That inhibitor also more effectively inhibited by bounding to jararhagin spread in blood vessels after snakebite because of it’s position and more positive binding energy (-784.1 kcal/mol). However, unfavorable bonds are formed in the interaction between batimastat inhibitors, jararhagin and integrin 2 1 . In inhibitor EDTA interaction, it was found that this compound also more easily bound to jararhagin (-227.23 kcal/mol), but this inhibitor are more effectively inhibited by bounding to integrin 2 1 because of it’s position and more positive binding energy (-721.57 kcal/mol). In other side it also has unfavorable bonds. While the interaction of hydroxytyrosol shows that inhibitor are easier to interact with jararhagin and more effectively acts as a jararhagin inhibitor by being consumed after the body is exposed to jararhagin (-781.33 kcal/mol) without showing an unfavorable bond. We can conclude that the natural inhibitors formed in hydroxytyrosol from olive oil are more stable and have highest possibility in preventing hemorrhagic symptoms
{"title":"In Silico Prediction of High Potential Jararhagin Inhibitor: Comparison of Batimastat, EDTA and Hydroxytyrosol","authors":"C. A. Kurniasari, Bayu D. Prakoso, Eka Lestari, N. Kurniawan","doi":"10.5220/0009586800050014","DOIUrl":"https://doi.org/10.5220/0009586800050014","url":null,"abstract":": One example of a group P-III SVMP is jararhagin which originates from a Bothrops jararaca . This study was conducted to compare the possibility of inhibitors that have the highest effectiveness and exact time of each compound to inhibit hemorragic effect of SVMP. Inhibition of hemorrhagic activity can be done with several types of compounds that have been known to be inhibitors for SVMP especially jararhagin (PDB ID: 1C9G) to bind with integrin 2 1 (PDB ID: 1AOX). There are batimastat (PubChem ID: 5362422) as one of peptidomimetic compounds, EDTA (PubChem ID: 6049) as one of zinc chelating agents, and plant compounds such as hydroxytyrosol (PubChem ID: 82755). The batimastat inhibitory properties from value of binding energy, found that these inhibitor were more easily bound to jararhagin (-289.0 kcal/mol) compared to integrin 2 1 (-277.1 kcal/mol). That inhibitor also more effectively inhibited by bounding to jararhagin spread in blood vessels after snakebite because of it’s position and more positive binding energy (-784.1 kcal/mol). However, unfavorable bonds are formed in the interaction between batimastat inhibitors, jararhagin and integrin 2 1 . In inhibitor EDTA interaction, it was found that this compound also more easily bound to jararhagin (-227.23 kcal/mol), but this inhibitor are more effectively inhibited by bounding to integrin 2 1 because of it’s position and more positive binding energy (-721.57 kcal/mol). In other side it also has unfavorable bonds. While the interaction of hydroxytyrosol shows that inhibitor are easier to interact with jararhagin and more effectively acts as a jararhagin inhibitor by being consumed after the body is exposed to jararhagin (-781.33 kcal/mol) without showing an unfavorable bond. We can conclude that the natural inhibitors formed in hydroxytyrosol from olive oil are more stable and have highest possibility in preventing hemorrhagic symptoms","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73147909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009587100150018
G. Ulfa, Regita Prihatiningtyas, Irma Nopriyani, W. Putri, K. Fibrianto, S. Widjanarko
Starch is a common material for tablet formulation as disintegrant, binder, or filler, however, it needs to be modify to improve its native properties. Physical modification by heat is one of the easiest way to modify the starch in tablet formulation. Sweet potato starch were pre-gelatinized and ultrasound to improve some physical properties of starch. The aim of this study was to compare the properties of starch produce using two different treatment. The swelling power (SP), solubility (S), and water binding capacity (WBC) of starches are the properties which important in the usage of starch in tablet formulation. The increase of all parameters measured could be important for controlled drug delivery. The granule of starches were also analysed using scanning electron microscopy to determine the effect of different treatments toward starch granules.
{"title":"Preliminary Comparison of Pregelatinization vs Ultrasound Modified Sweet Potato Starch for Tablet Formulation","authors":"G. Ulfa, Regita Prihatiningtyas, Irma Nopriyani, W. Putri, K. Fibrianto, S. Widjanarko","doi":"10.5220/0009587100150018","DOIUrl":"https://doi.org/10.5220/0009587100150018","url":null,"abstract":"Starch is a common material for tablet formulation as disintegrant, binder, or filler, however, it needs to be modify to improve its native properties. Physical modification by heat is one of the easiest way to modify the starch in tablet formulation. Sweet potato starch were pre-gelatinized and ultrasound to improve some physical properties of starch. The aim of this study was to compare the properties of starch produce using two different treatment. The swelling power (SP), solubility (S), and water binding capacity (WBC) of starches are the properties which important in the usage of starch in tablet formulation. The increase of all parameters measured could be important for controlled drug delivery. The granule of starches were also analysed using scanning electron microscopy to determine the effect of different treatments toward starch granules.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77503036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009588200680073
Ermi Girsang, I. Lister, C. Ginting, Sri Lestari Ramadhani Nasution, S. Suhartina, U. Z. Munshy, R. Rizal, W. Widowati
: Lead toxicity is a serious environmental disease and its effects on the human body are overwhelming. Lead can increase reactive oxygen species (ROS) levels in the body which results in oxidative stress. Elevated ROS levels can stimulate inflammation and cellular aging. Plants extract have the abilities as antioxidant and anti-inflammatory agent to prevent aging and toxicity including Salacca zalacca peels extract (SPE). Cytotoxicity assay of SPE towards fibroblast cells (BJ) was handle using MTS (3-4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). Intracellular ROS levels were observed by flow cytometry using DCF-DA fluorescent probe. Fibroblast cells were incubated at 37 0 C, 5% CO 2 , treated by 25 and 100 µg/ml SPE for 4 hours and followed by 400 µM Pb for 72 hours. Anti-inflammatory capacity was conducted using ELISA to measure IL-10 and TNF- α . SPE at 3.13-100 µg/ml were nontoxic to the BJ cells. Accumulation of intracellular ROS levels in lead-induced BJ cells were decreased by treatment using SPE at 25 and 100 µg/ml. SPE at 25 and 100 µg/ml elevated IL-10 and decreased TNF- α related to positive control (lead-induced cells). This research shows that S. zalacca peels extract has the ability as protective effect related to Pb poisoning.
{"title":"Antioxidant and Anti-inflammatory Activity of Salacca zalacca (Gaertn.) Voss Peel Ethanolic Extract on Lead Induced Fibroblast Cells","authors":"Ermi Girsang, I. Lister, C. Ginting, Sri Lestari Ramadhani Nasution, S. Suhartina, U. Z. Munshy, R. Rizal, W. Widowati","doi":"10.5220/0009588200680073","DOIUrl":"https://doi.org/10.5220/0009588200680073","url":null,"abstract":": Lead toxicity is a serious environmental disease and its effects on the human body are overwhelming. Lead can increase reactive oxygen species (ROS) levels in the body which results in oxidative stress. Elevated ROS levels can stimulate inflammation and cellular aging. Plants extract have the abilities as antioxidant and anti-inflammatory agent to prevent aging and toxicity including Salacca zalacca peels extract (SPE). Cytotoxicity assay of SPE towards fibroblast cells (BJ) was handle using MTS (3-4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). Intracellular ROS levels were observed by flow cytometry using DCF-DA fluorescent probe. Fibroblast cells were incubated at 37 0 C, 5% CO 2 , treated by 25 and 100 µg/ml SPE for 4 hours and followed by 400 µM Pb for 72 hours. Anti-inflammatory capacity was conducted using ELISA to measure IL-10 and TNF- α . SPE at 3.13-100 µg/ml were nontoxic to the BJ cells. Accumulation of intracellular ROS levels in lead-induced BJ cells were decreased by treatment using SPE at 25 and 100 µg/ml. SPE at 25 and 100 µg/ml elevated IL-10 and decreased TNF- α related to positive control (lead-induced cells). This research shows that S. zalacca peels extract has the ability as protective effect related to Pb poisoning.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89667396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009586701220125
C. Mahdi, A. Haskito, M. Padaga, A. Roosdiana
: Dioxins are highly toxic and carcinogenic compounds. The most dangerous dioxin is 2,3,7,8-tetrachloro-dibenzo-p-diozin (TCDD). TCDD can cause health problem accumulates overtime. Goat milk yoghurtcasein can reduce free radicals caused by TCDD. Goat milk yoghurt casein contains bioactive peptide that acts as antioxidant. The aim of the current research was to determine the effect of goat milk yoghurt towards TNF-α and IL1- β level after TCDD exposure. This research was an experimental study using a completely randomized design (CRD). Rattus norvegicus were divided into 6 groups: K- (negative control), K+ (positive control induced by TCDD 100 mg/kgBW), KP (placebo control given goat milk yoghurt casein 300 mg/kgBB), K1 (given TCDD 100 mg/kgBW and goat milk yoghurt casein 300 mg/kgBB), K2 (given TCDD 100 mg/kgBW and goat milk yoghurt casein 600 mg/kgBB), and K3 (given TCDD 100 mg/kgBW and goat milk yoghurt casein 900 mg/kgBB). Quantitative analysis withone-way ANOVA and post hoc Tukey test showed that goat milk yoghurt could prevent TNF- α increase significantly (p<0,05) from control groups, and also prevent IL1- β increase although not significantly different from control groups (p>0,05). It can be concluded that goat milk yoghurt casein 900 mg/kgBW can prevent the increase of TNF- α and IL1- β . Thus, goat milk yoghurt casein could be used as antioxidant source against TCDD exposure.
{"title":"The Effect of Goat Milk Yoghurt Casein Antioxidant Activity on KidneyTumor Necrosis Factor Alpha (TNF-α) and Interleukin 1 Beta (IL-1β) Levels in RattusnorvegicusExposed by 2,3,7,8-tetrachlorodibenzo-p- dioxin (TCDD)","authors":"C. Mahdi, A. Haskito, M. Padaga, A. Roosdiana","doi":"10.5220/0009586701220125","DOIUrl":"https://doi.org/10.5220/0009586701220125","url":null,"abstract":": Dioxins are highly toxic and carcinogenic compounds. The most dangerous dioxin is 2,3,7,8-tetrachloro-dibenzo-p-diozin (TCDD). TCDD can cause health problem accumulates overtime. Goat milk yoghurtcasein can reduce free radicals caused by TCDD. Goat milk yoghurt casein contains bioactive peptide that acts as antioxidant. The aim of the current research was to determine the effect of goat milk yoghurt towards TNF-α and IL1- β level after TCDD exposure. This research was an experimental study using a completely randomized design (CRD). Rattus norvegicus were divided into 6 groups: K- (negative control), K+ (positive control induced by TCDD 100 mg/kgBW), KP (placebo control given goat milk yoghurt casein 300 mg/kgBB), K1 (given TCDD 100 mg/kgBW and goat milk yoghurt casein 300 mg/kgBB), K2 (given TCDD 100 mg/kgBW and goat milk yoghurt casein 600 mg/kgBB), and K3 (given TCDD 100 mg/kgBW and goat milk yoghurt casein 900 mg/kgBB). Quantitative analysis withone-way ANOVA and post hoc Tukey test showed that goat milk yoghurt could prevent TNF- α increase significantly (p<0,05) from control groups, and also prevent IL1- β increase although not significantly different from control groups (p>0,05). It can be concluded that goat milk yoghurt casein 900 mg/kgBW can prevent the increase of TNF- α and IL1- β . Thus, goat milk yoghurt casein could be used as antioxidant source against TCDD exposure.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88485180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009587801470151
N. Titisari, Theresa Lidya Pramesti, T. Widyaputri, Rahadi Swastomo
: Sumbawa horse milk contains bioactive components as exogenous antioxidants, anti-inflammatory andantimicrobial which can prevent Inflammatory Bowel Disease. IBD is characterized by ongoing inflammation of the digestive tract that could changed gastric histology and trigger pro-inflammatory cytokines. The purpose of this study was to observe the preventive effects of Sumbawa horse milk on gastric organs inflammation in IBD animal models. Twenty male rats, 8 weeks, 150-200 grams BW, divided into five treatment groups; K- group (negative control), indometacin induced group; i.e K+ (positive group without horse milk), P1(horse milk dose 0.5 mL/rat), P2 (horse milk dose1.0 mL/rat), and P3(horse milk dose 1.5mL/rat). Flowcytometry method was conducted to observe IL-6 expression, and then analyzed with one way ANOVA followed by a tukey test (α 95%). While the gastric histopathological was analyzed descriptively. The results showed that IL-6 expression were significantly lower (p <0.05) in P2 dan P3 compared with K+group. Whereas in the histological result, the p3 group was able to prevent histopathological damage of the gastric organs compared to other treatment groups. The conclusion of the study is Sumbawa horse milk able to prevent increased of IL-6 and gastric mucosal cell erosion, with the best dose is 1.5 mL/rat.
{"title":"Sumbawa Horse Milk as a Prevention of Inflammatory Bowel Disease (IBD) in Animal Model based on IL-6 Expression and Gastric Histopathology","authors":"N. Titisari, Theresa Lidya Pramesti, T. Widyaputri, Rahadi Swastomo","doi":"10.5220/0009587801470151","DOIUrl":"https://doi.org/10.5220/0009587801470151","url":null,"abstract":": Sumbawa horse milk contains bioactive components as exogenous antioxidants, anti-inflammatory andantimicrobial which can prevent Inflammatory Bowel Disease. IBD is characterized by ongoing inflammation of the digestive tract that could changed gastric histology and trigger pro-inflammatory cytokines. The purpose of this study was to observe the preventive effects of Sumbawa horse milk on gastric organs inflammation in IBD animal models. Twenty male rats, 8 weeks, 150-200 grams BW, divided into five treatment groups; K- group (negative control), indometacin induced group; i.e K+ (positive group without horse milk), P1(horse milk dose 0.5 mL/rat), P2 (horse milk dose1.0 mL/rat), and P3(horse milk dose 1.5mL/rat). Flowcytometry method was conducted to observe IL-6 expression, and then analyzed with one way ANOVA followed by a tukey test (α 95%). While the gastric histopathological was analyzed descriptively. The results showed that IL-6 expression were significantly lower (p <0.05) in P2 dan P3 compared with K+group. Whereas in the histological result, the p3 group was able to prevent histopathological damage of the gastric organs compared to other treatment groups. The conclusion of the study is Sumbawa horse milk able to prevent increased of IL-6 and gastric mucosal cell erosion, with the best dose is 1.5 mL/rat.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73372239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009587300620067
H. Pratiwi, A. Firmawati, Diana Rahmayani Putri, A. Haryo, A. W. Wardhana
Ovarian hypofunction is pathologic conditions where is the ovary being abnormal. The abnormality of the ovary can be induced by the abnormality of the endocrine that regulates the development of the ovary such as, follicle-stimulating hormone (FSH) and luteinizing hormone LH. The production of FSH and LH in the pituitary is determined with the Gonadotropin hormone (GnRH) stimulation. Development of rat ovarian hypofunction models can be performed with the induction of cetrorelix acetate which has an antagonist effect of GnRH. This research was conduct to know the effect of induction of cetrorelix acetate on rat oviduct estrogen beta receptor expression and histopathology. The study used three groups of female rats (Wistar strain) 8-10 weeks old and 150-180 gram weight, each group consisting of six rats. The first group (control) without cetrorelix acetate, the second group treated with cetrorelix acetate 0.009 mg/kg BW and the third group treated with cetrorelix acetate 0.0135 mg/kg BW. Observations of estrogen beta receptor expressions (ERs β) are carried out with immunochemical methods, while observations of histopathological changes of oviduct carried out by Hematocsilin-Eosin (HE stain). The results obtained indicate a significant difference from the administration of the GnRH antagonists in the three treatment groups, among others, the largest reduction of the expression of the estrogen receptor of the ES β by 59.2%, as well as the thinning of the fallopian tubes and the reduced cilia. The conclusion of the study was Cetrorelix acetate as a GnRH antagonist capable of lowering the beta estrogen receptor expression and reducing the number of cilia as well as the viscosity of the wall lining of the fallopian tubes.
{"title":"Interventions of Cetrorelix Acetate in Estrogen Beta Receptor Expression and Histopathology in Rats Oviduct","authors":"H. Pratiwi, A. Firmawati, Diana Rahmayani Putri, A. Haryo, A. W. Wardhana","doi":"10.5220/0009587300620067","DOIUrl":"https://doi.org/10.5220/0009587300620067","url":null,"abstract":"Ovarian hypofunction is pathologic conditions where is the ovary being abnormal. The abnormality of the ovary can be induced by the abnormality of the endocrine that regulates the development of the ovary such as, follicle-stimulating hormone (FSH) and luteinizing hormone LH. The production of FSH and LH in the pituitary is determined with the Gonadotropin hormone (GnRH) stimulation. Development of rat ovarian hypofunction models can be performed with the induction of cetrorelix acetate which has an antagonist effect of GnRH. This research was conduct to know the effect of induction of cetrorelix acetate on rat oviduct estrogen beta receptor expression and histopathology. The study used three groups of female rats (Wistar strain) 8-10 weeks old and 150-180 gram weight, each group consisting of six rats. The first group (control) without cetrorelix acetate, the second group treated with cetrorelix acetate 0.009 mg/kg BW and the third group treated with cetrorelix acetate 0.0135 mg/kg BW. Observations of estrogen beta receptor expressions (ERs β) are carried out with immunochemical methods, while observations of histopathological changes of oviduct carried out by Hematocsilin-Eosin (HE stain). The results obtained indicate a significant difference from the administration of the GnRH antagonists in the three treatment groups, among others, the largest reduction of the expression of the estrogen receptor of the ES β by 59.2%, as well as the thinning of the fallopian tubes and the reduced cilia. The conclusion of the study was Cetrorelix acetate as a GnRH antagonist capable of lowering the beta estrogen receptor expression and reducing the number of cilia as well as the viscosity of the wall lining of the fallopian tubes.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85325545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009586400410048
A. Firmawati, M. Hutabarat, H. Pratiwi
: One of the reproductive disorders that are often found in breeders in Indonesia is ovarian hypofunction. Ovarian hypofunction is a pathological condition in the ovary that is characterized by a decrease in ovarian function that causes inhibition of folliculogenesis and failure of ovulation. The purpose of this study was to develop ovarian hypofunction animal models through cetrorelix acetate induction and observe their effects on the expression of estrogen beta receptors (Ers β ) and histopathological changes in the ovaries. This study used three groups of female Wistar strains (Rattus norvegicus), with ages 8-10 weeks, and body weight 150-180 grams. The treatments in this study included a control group (KN) without cetrorelix acetate, the first group (P1) was treatment group with an injection of cetrorelix acetate 0.009 mg/kg BW, and the second group (P2) was treatment group with an injection of cetrorelix acetate exposure 0.0135 mg/kg BW. Expression of beta estrogen receptors (Ers β ) in the ovaries was analyzed by immunohistochemical methods, and the data were analyzed using the BNJ test (p <0.05). The ovarian histopathological changes were analyzed by the hematoxylin-eosin (HE) staining method, then analyzed qualitatively. The results of this study indicate that the treatment groups P1 and P2 with GnRH antagonists differ significantly compared to the negative control group. The P2 treatment group had the highest reduction in estrogen receptor expression with Ers β by 92.2%. The result of histopathological in P1 and P2 treatment groups were able to inhibit the development of antral follicles. The conclusion of this study is that cetrorelix acetate as GnRH antagonists can reduce estrogen receptor expression and inhibit folliculogenesis in ovarian histopathology.
{"title":"Expression of Estrogen Beta (ERs β) Receptor and Ovarian Histopathology Changes in Rats (Rattus norvegicus) Ovarian Hypofunction Model","authors":"A. Firmawati, M. Hutabarat, H. Pratiwi","doi":"10.5220/0009586400410048","DOIUrl":"https://doi.org/10.5220/0009586400410048","url":null,"abstract":": One of the reproductive disorders that are often found in breeders in Indonesia is ovarian hypofunction. Ovarian hypofunction is a pathological condition in the ovary that is characterized by a decrease in ovarian function that causes inhibition of folliculogenesis and failure of ovulation. The purpose of this study was to develop ovarian hypofunction animal models through cetrorelix acetate induction and observe their effects on the expression of estrogen beta receptors (Ers β ) and histopathological changes in the ovaries. This study used three groups of female Wistar strains (Rattus norvegicus), with ages 8-10 weeks, and body weight 150-180 grams. The treatments in this study included a control group (KN) without cetrorelix acetate, the first group (P1) was treatment group with an injection of cetrorelix acetate 0.009 mg/kg BW, and the second group (P2) was treatment group with an injection of cetrorelix acetate exposure 0.0135 mg/kg BW. Expression of beta estrogen receptors (Ers β ) in the ovaries was analyzed by immunohistochemical methods, and the data were analyzed using the BNJ test (p <0.05). The ovarian histopathological changes were analyzed by the hematoxylin-eosin (HE) staining method, then analyzed qualitatively. The results of this study indicate that the treatment groups P1 and P2 with GnRH antagonists differ significantly compared to the negative control group. The P2 treatment group had the highest reduction in estrogen receptor expression with Ers β by 92.2%. The result of histopathological in P1 and P2 treatment groups were able to inhibit the development of antral follicles. The conclusion of this study is that cetrorelix acetate as GnRH antagonists can reduce estrogen receptor expression and inhibit folliculogenesis in ovarian histopathology.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88901257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009587000530057
F. S. Permata, D. A. Pratama, A. Haryo
Immunohistochemistry (IHC) is a popular method to detect protein in the tissue. But, determining protein level in tissue is using ELISA which needs more cost. Although, the intensity of IHC color relates to an amount in tissue. Therefore, image processing to measure the intensity of IHC quantitively was developed using much software. The common software used in Indonesia is Corel Photo-Paint® (CCP). This article described the simple method to measure the IHC intensity using CCP based on Cyan-Magenta-YellowBlack (CMYK) Split channel. The conclusions were CCP could be used for image processing software to measure the IHC intensity. The higher intensity has fewer grey level, the lower intensity has a higher grey level
{"title":"The Simple Method for Immunohistochemistry (IHC) Analyzing of Intensity using Corel Photo-Paint® with CMYK Split-channel","authors":"F. S. Permata, D. A. Pratama, A. Haryo","doi":"10.5220/0009587000530057","DOIUrl":"https://doi.org/10.5220/0009587000530057","url":null,"abstract":"Immunohistochemistry (IHC) is a popular method to detect protein in the tissue. But, determining protein level in tissue is using ELISA which needs more cost. Although, the intensity of IHC color relates to an amount in tissue. Therefore, image processing to measure the intensity of IHC quantitively was developed using much software. The common software used in Indonesia is Corel Photo-Paint® (CCP). This article described the simple method to measure the IHC intensity using CCP based on Cyan-Magenta-YellowBlack (CMYK) Split channel. The conclusions were CCP could be used for image processing software to measure the IHC intensity. The higher intensity has fewer grey level, the lower intensity has a higher grey level","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90947545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5220/0009586100890095
Ahmad Muammar Kadafi, M. Fathoni, M. A. Fauzi, Richo Firmansyah, B. Priambodo, N. Kurniawan
: Kondang Merak Forest was known for its tourism destination, despite this area being the only coastal protected forest in Malang. Because of tourism demand, several anthropogenic activities were recorded and threatening the ecological balances of the forest including herpetofauna in it. Hence, we provide the information on the herpetofauna diversity completed with the habitat type on the Kondang Merak forest. A survey was conducted from November 2018 to June 2019 on each 6 different site points according to a specific habitat. We analyzed the data using the importance value index and Jaccard similarity to better known the herpetofauna community structure. A total of 38 species of herpetofauna were recorded consists of 8 amphibians (Anura), 15 lizards (Sauria), and 15 snakes (Serpentes) species. Based on the habitat type, we concluded that the distribution pattern and herpetofauna composition may be reflected by the biological adaptation, environmental carrying capacity, and different landscape vegetation such as coastal area, stream, temporary pond, secondary forest and monoculture plantation. These findings can be used as baseline data for further study on genetic study and management strategies on the Kondang Merak forest.
{"title":"Study of Species Richness and Structure Community of Herpetofauna on Kondang Merak Forest, Malang, Indonesia","authors":"Ahmad Muammar Kadafi, M. Fathoni, M. A. Fauzi, Richo Firmansyah, B. Priambodo, N. Kurniawan","doi":"10.5220/0009586100890095","DOIUrl":"https://doi.org/10.5220/0009586100890095","url":null,"abstract":": Kondang Merak Forest was known for its tourism destination, despite this area being the only coastal protected forest in Malang. Because of tourism demand, several anthropogenic activities were recorded and threatening the ecological balances of the forest including herpetofauna in it. Hence, we provide the information on the herpetofauna diversity completed with the habitat type on the Kondang Merak forest. A survey was conducted from November 2018 to June 2019 on each 6 different site points according to a specific habitat. We analyzed the data using the importance value index and Jaccard similarity to better known the herpetofauna community structure. A total of 38 species of herpetofauna were recorded consists of 8 amphibians (Anura), 15 lizards (Sauria), and 15 snakes (Serpentes) species. Based on the habitat type, we concluded that the distribution pattern and herpetofauna composition may be reflected by the biological adaptation, environmental carrying capacity, and different landscape vegetation such as coastal area, stream, temporary pond, secondary forest and monoculture plantation. These findings can be used as baseline data for further study on genetic study and management strategies on the Kondang Merak forest.","PeriodicalId":20550,"journal":{"name":"Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81049308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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