The calculation of neutron activation source terms is an essential task in the radiation of nuclear reactors, and its sensitivity and uncertainty (S&U) analysis can help better identify and control the influencing factors and safety margin of activation source terms. In this paper, we studied the statistical sampling strategies for the S&U analysis of activation source terms, established the calculation process of sensitivity coefficient and uncertainty, and the S&U analysis module based on sampling methods was integrated for the proprietary developed activation computation program ABURN. Two sampling ways, Monte Carlo and Latin hypercube sampling treatments, were employed to figure out the cases of pure decay single chain, pure activation single chain, and complex reaction network. The sensitivity coefficients and uncertainty of nuclide inventory were evaluated by taking the uncertainty of decay constant or reaction cross-section into account. The simulation results indicate that after introducing the LHS sampling method, the activation calculation program ABURN can improve computational efficiency in sensitivity and uncertainty analysis. Subsequent results demonstrated that the sensitivity coefficients and uncertainty solutions based on the sampling methods in the ABURN program were in good agreement with the analytical solutions and the FISPACT program, which verified the accuracy of the approaches and program in this paper.