Pub Date : 2014-01-01DOI: 10.3724/SP.J.1206.2014.00036
Jun-Ping Liu
Aging occurs with cell beginning to lose function in a variety of cell types. If it takes place in a given tissue or organ prematurely ahead of other tissues and organs,it causes pathological development of diseases. How to treat aging cells and associated molecules is a challenge in anti-aging research. It has been demonstrated recently that aging occurs under physiological conditions during embryonic development of mammals,and that to some life forms aging never occurs. Involved in diverse diseases,aging is difficult to be measured and does not have universal markers. It is thus essential to comprehend the types of cells that undergo aging in human diseases and thereby the underlying molecular mechanisms. This article discusses recent research advances including:(1) The concept,classification and relevant mechanisms of cell aging;(2) Physiological aging: Developmentally programmed senescence;(3) Tissue homeostasis and aging;(4) Cell replicative senescence and related diseases: telomeres and cancer prognosis,idiopathic pulmonary fibrosis,hypertension;(5) Non-replicative cell aging and related diseases: Parkinson disease and diabetes;(6) Species diversity in aging and longevity.
{"title":"Aging and related diseases: Research progress","authors":"Jun-Ping Liu","doi":"10.3724/SP.J.1206.2014.00036","DOIUrl":"https://doi.org/10.3724/SP.J.1206.2014.00036","url":null,"abstract":"Aging occurs with cell beginning to lose function in a variety of cell types. If it takes place in a given tissue or organ prematurely ahead of other tissues and organs,it causes pathological development of diseases. How to treat aging cells and associated molecules is a challenge in anti-aging research. It has been demonstrated recently that aging occurs under physiological conditions during embryonic development of mammals,and that to some life forms aging never occurs. Involved in diverse diseases,aging is difficult to be measured and does not have universal markers. It is thus essential to comprehend the types of cells that undergo aging in human diseases and thereby the underlying molecular mechanisms. This article discusses recent research advances including:(1) The concept,classification and relevant mechanisms of cell aging;(2) Physiological aging: Developmentally programmed senescence;(3) Tissue homeostasis and aging;(4) Cell replicative senescence and related diseases: telomeres and cancer prognosis,idiopathic pulmonary fibrosis,hypertension;(5) Non-replicative cell aging and related diseases: Parkinson disease and diabetes;(6) Species diversity in aging and longevity.","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":"19 1","pages":"215-230"},"PeriodicalIF":0.3,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86233138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-01-01DOI: 10.3724/SP.J.1206.2012.00613
Gong Zj, Ming Zhou, Peng Sp, J. Ma, Xiaoyan Li, Hongbin Huang, Hongbin Huang, Y. Li, Xiaoling Li, H. Bo, Zeng Zy, Strong, Gui-yuan Li, Xiang Jj, W. Xiong, F. Wei, Zhang Wl, Liao Qj, K. Tang, Song Yl
Recently, we sequenced the transcriptomes of a hepatocellular carcinoma biopsy and a normal liver tissue using the RNA-Sequencing(RNA-Seq) strategy based on the Next Generation Sequencing(NGS) technique, and identified several adjacent high RNA-Seq signal peaks on chromosome 11q13.1 in the hepatocellular carcinoma biopsy, while not in the normal control tissue. In this chromosome region, there is no characterized genes have been identified, implying that these RNA-Seq peaks may represent one or more novel genes. Further study was confirmed that these RNA-Seq peaks were transcribed by one novel gene. Through cloning the full length of this novel gene, we found that this novel gene transcribed many splicing isoforms, and the longest isoform is 3 562 bp. Then we deposited twelve representative RNA isoforms into the GenBank database of the National Center for Biotechnology Information(NCBI), and created the GenBank IDs from KC136297 to KC136308 for these isoforms. None significant open reading fragment(ORF) was found in any transcripts of this novel gene, implying that this gene may encodes long non-coding RNAs(lncRNAs). To further elucidate the potential transcriptional regulation mechanism of this lncRNA gene, we predicted the promoter from the upstream sequence of the lncRNA gene using bioinformatic tools, and found that there is one potential promoter in-719 to-469 bp from the transcript start site of the lncRNA gene, and there are seven Sp1, one STAT5 and one EGR1 transcription factor binding sites in the promoter region. The molecular mechanisms of the lncRNA gene in carcinogenesis and progression of hepatocellular carcinoma are worthful for further investigation.
{"title":"Cloning and Functional Characterization of a Novel Long Non-coding RNA Gene Associated With Hepatocellular Carcinoma","authors":"Gong Zj, Ming Zhou, Peng Sp, J. Ma, Xiaoyan Li, Hongbin Huang, Hongbin Huang, Y. Li, Xiaoling Li, H. Bo, Zeng Zy, Strong, Gui-yuan Li, Xiang Jj, W. Xiong, F. Wei, Zhang Wl, Liao Qj, K. Tang, Song Yl","doi":"10.3724/SP.J.1206.2012.00613","DOIUrl":"https://doi.org/10.3724/SP.J.1206.2012.00613","url":null,"abstract":"Recently, we sequenced the transcriptomes of a hepatocellular carcinoma biopsy and a normal liver tissue using the RNA-Sequencing(RNA-Seq) strategy based on the Next Generation Sequencing(NGS) technique, and identified several adjacent high RNA-Seq signal peaks on chromosome 11q13.1 in the hepatocellular carcinoma biopsy, while not in the normal control tissue. In this chromosome region, there is no characterized genes have been identified, implying that these RNA-Seq peaks may represent one or more novel genes. Further study was confirmed that these RNA-Seq peaks were transcribed by one novel gene. Through cloning the full length of this novel gene, we found that this novel gene transcribed many splicing isoforms, and the longest isoform is 3 562 bp. Then we deposited twelve representative RNA isoforms into the GenBank database of the National Center for Biotechnology Information(NCBI), and created the GenBank IDs from KC136297 to KC136308 for these isoforms. None significant open reading fragment(ORF) was found in any transcripts of this novel gene, implying that this gene may encodes long non-coding RNAs(lncRNAs). To further elucidate the potential transcriptional regulation mechanism of this lncRNA gene, we predicted the promoter from the upstream sequence of the lncRNA gene using bioinformatic tools, and found that there is one potential promoter in-719 to-469 bp from the transcript start site of the lncRNA gene, and there are seven Sp1, one STAT5 and one EGR1 transcription factor binding sites in the promoter region. The molecular mechanisms of the lncRNA gene in carcinogenesis and progression of hepatocellular carcinoma are worthful for further investigation.","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":"85 1","pages":"153-162"},"PeriodicalIF":0.3,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78171140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-07-01DOI: 10.3724/SP.J.1206.2013.00079
Junye Miao, Jing Lu, Ziang Zhang, Zhiqian Tong, R. He
A certain concentration of formaldehyde can cause protein misfolding, cell death and biological dysfunction. Though it has been reported that formaldehyde has cytotoxicity, how formaldehyde affects cell cycle of neural cells and the molecular mechanism still needs to be clarified. In this study, neuroblastoma cell line SH-SY5Y was utilized to incubate with formaldehyde and the effect of formaldehyde on cell cycle was in formaldehyde concentration-dependent manner. No significant changes in cell cycle could be detected when[FA] ≤0.1 mmol/L (cells were incubated for 48 h), while the percentages of cells in S phase and G2/M phase were markedly increased with the elevation of formaldehyde concentration (0.1 mmol/L < [FA] ≤ 0.2 mmol/L). In the medium with 0.3 mmol/L formaldehyde, 46.28% of cells were in S phase while only 16.05% of them were in G2/M phase, that is, cell proliferation was obviously inhibited under the conditions. When cells were synchronized at G2/M phase, formaldehyde (0.1∼0.3 mmol/L) could markedly increase the number of cells in S phase, though, to some extent, the number of cells in G2/M phase decreased. When cells were synchronized at S phase, 0.1 mmol/L formaldehyde could decrease the number of cells in G2/M phase, while 0.3 mmol/L formaldehyde could markedly decrease the number of cells in G2/M phase and significantly increase that in S phase. In the presence of formaldehyde, primary neurons of SD rat exhibited similar changes in cell cycle as that in SH-SY5 Y cells. Furthermore, early and late apoptosis was markedly observed when 0.1 mmol/L ≤ [FA] ≤0.2 mmol/L, while DNA were obviously damaged and most cells were apoptosis and some of them underwent necrosis when [FA] ≤ 0.3 mmol/L. In sum, formaldehyde at a low concentration (0.1 mmol/L≤ [FA] ≤0.2 mmol/L) mainly suppresses DNA synthesis in S phase via hypermethylation of global DNA, while formaldehyde at a higher concentration ([FA] ≥ 0.3 mmol/L) causes DNA damage, both of them lead to the aberrant effects on cell cycle.
{"title":"The effect of formaldehyde on cell cycle is in a concentration-dependent manner","authors":"Junye Miao, Jing Lu, Ziang Zhang, Zhiqian Tong, R. He","doi":"10.3724/SP.J.1206.2013.00079","DOIUrl":"https://doi.org/10.3724/SP.J.1206.2013.00079","url":null,"abstract":"A certain concentration of formaldehyde can cause protein misfolding, cell death and biological dysfunction. Though it has been reported that formaldehyde has cytotoxicity, how formaldehyde affects cell cycle of neural cells and the molecular mechanism still needs to be clarified. In this study, neuroblastoma cell line SH-SY5Y was utilized to incubate with formaldehyde and the effect of formaldehyde on cell cycle was in formaldehyde concentration-dependent manner. No significant changes in cell cycle could be detected when[FA] ≤0.1 mmol/L (cells were incubated for 48 h), while the percentages of cells in S phase and G2/M phase were markedly increased with the elevation of formaldehyde concentration (0.1 mmol/L < [FA] ≤ 0.2 mmol/L). In the medium with 0.3 mmol/L formaldehyde, 46.28% of cells were in S phase while only 16.05% of them were in G2/M phase, that is, cell proliferation was obviously inhibited under the conditions. When cells were synchronized at G2/M phase, formaldehyde (0.1∼0.3 mmol/L) could markedly increase the number of cells in S phase, though, to some extent, the number of cells in G2/M phase decreased. When cells were synchronized at S phase, 0.1 mmol/L formaldehyde could decrease the number of cells in G2/M phase, while 0.3 mmol/L formaldehyde could markedly decrease the number of cells in G2/M phase and significantly increase that in S phase. In the presence of formaldehyde, primary neurons of SD rat exhibited similar changes in cell cycle as that in SH-SY5 Y cells. Furthermore, early and late apoptosis was markedly observed when 0.1 mmol/L ≤ [FA] ≤0.2 mmol/L, while DNA were obviously damaged and most cells were apoptosis and some of them underwent necrosis when [FA] ≤ 0.3 mmol/L. In sum, formaldehyde at a low concentration (0.1 mmol/L≤ [FA] ≤0.2 mmol/L) mainly suppresses DNA synthesis in S phase via hypermethylation of global DNA, while formaldehyde at a higher concentration ([FA] ≥ 0.3 mmol/L) causes DNA damage, both of them lead to the aberrant effects on cell cycle.","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":"439 1","pages":"641-651"},"PeriodicalIF":0.3,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82881623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-08DOI: 10.3724/SP.J.1206.2012.00140
Xiao-Yan Liu, Qian Lu, Wu-jun Chen, Chao-ke Tang
{"title":"New Research Advances in Genetics Associated With High-density Lipoprotein Cholesterol*: New Research Advances in Genetics Associated With High-density Lipoprotein Cholesterol*","authors":"Xiao-Yan Liu, Qian Lu, Wu-jun Chen, Chao-ke Tang","doi":"10.3724/SP.J.1206.2012.00140","DOIUrl":"https://doi.org/10.3724/SP.J.1206.2012.00140","url":null,"abstract":"","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":"19 1","pages":"1145-1155"},"PeriodicalIF":0.3,"publicationDate":"2013-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76753661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-08DOI: 10.3724/SP.J.1206.2012.00124
Wenping Luo, Dong-mei Tan, Genling Yang, Junjie Lu, Hai Zhao, Yi Tan
{"title":"Effects of GABA Signal on Mouse Placenta Establishment in Early-Middle Phase*: Effects of GABA Signal on Mouse Placenta Establishment in Early-Middle Phase*","authors":"Wenping Luo, Dong-mei Tan, Genling Yang, Junjie Lu, Hai Zhao, Yi Tan","doi":"10.3724/SP.J.1206.2012.00124","DOIUrl":"https://doi.org/10.3724/SP.J.1206.2012.00124","url":null,"abstract":"","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":"1 1","pages":"1207-1214"},"PeriodicalIF":0.3,"publicationDate":"2013-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85315524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-08DOI: 10.3724/SP.J.1206.2012.00202
Fu-xiang Zhu, Ze-long Liu, Jing Miao, Hui-ge Qu, Xiao-yan Chi
{"title":"Intein-Fused Lucine Zippers Increase Plasma Coagulation Activity by Improving Protein Trans -Splicing in Dual-Vector Factor VIII Gene Delivered Mice*: Intein-Fused Lucine Zippers Increase Plasma Coagulation Activity by Improving Protein Trans -Splicing in Dual-Vector Factor VIII Gene Delivered Mice*","authors":"Fu-xiang Zhu, Ze-long Liu, Jing Miao, Hui-ge Qu, Xiao-yan Chi","doi":"10.3724/SP.J.1206.2012.00202","DOIUrl":"https://doi.org/10.3724/SP.J.1206.2012.00202","url":null,"abstract":"","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":"39 1","pages":"1220-1225"},"PeriodicalIF":0.3,"publicationDate":"2013-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81562294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-08DOI: 10.3724/SP.J.1206.2012.00136
Xiaojuan Li, Zhenglu Jiang, Yi Wang
{"title":"The Temporal Responses of Neurons in The Primary Visual Cortex to Transient Stimuli*: The Temporal Responses of Neurons in The Primary Visual Cortex to Transient Stimuli*","authors":"Xiaojuan Li, Zhenglu Jiang, Yi Wang","doi":"10.3724/SP.J.1206.2012.00136","DOIUrl":"https://doi.org/10.3724/SP.J.1206.2012.00136","url":null,"abstract":"","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":"52 1","pages":"1190-1196"},"PeriodicalIF":0.3,"publicationDate":"2013-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72466669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-08DOI: 10.3724/SP.J.1206.2012.00125
M. Gao, Ying Zhang, Dacheng Wang
{"title":"Crystallization and Preliminary Crystallographic Studies of Active TNF-α-Inducing Protein From Helicobacter Pylori *: Crystallization and Preliminary Crystallographic Studies of Active TNF-α-Inducing Protein From Helicobacter Pylori *","authors":"M. Gao, Ying Zhang, Dacheng Wang","doi":"10.3724/SP.J.1206.2012.00125","DOIUrl":"https://doi.org/10.3724/SP.J.1206.2012.00125","url":null,"abstract":"","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":"78 1","pages":"1215-1219"},"PeriodicalIF":0.3,"publicationDate":"2013-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75000533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-08DOI: 10.3724/SP.J.1206.2012.00015
Zhaojian Gong, Hongbin Huang, Ke Xu, Fang Liang, L. Xiaoling, W. Xiong, Zhaoyang Zeng, Liu Guiyuan
{"title":"Advances in microRNAs and TP53 Gene Regulatory Network*: Advances in microRNAs and TP53 Gene Regulatory Network*","authors":"Zhaojian Gong, Hongbin Huang, Ke Xu, Fang Liang, L. Xiaoling, W. Xiong, Zhaoyang Zeng, Liu Guiyuan","doi":"10.3724/SP.J.1206.2012.00015","DOIUrl":"https://doi.org/10.3724/SP.J.1206.2012.00015","url":null,"abstract":"","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":"9 1","pages":"1133-1144"},"PeriodicalIF":0.3,"publicationDate":"2013-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74661140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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