Pub Date : 2024-08-08DOI: 10.18413/rrpharmacology.10.489
Николай Иннокентьевич Суслов, Юлия С. Федорова, Максим Л. Коробов
Введение. Болезнь Альцгеймера все чаще становится причиной ранней инвалидизацией и смерти. Попытки создания фармакотерапевтического средства, дающего эффективный результат в лечении этой патологии, до сегодняшнего дня не увенчались успехом. В данной статье приведены результаты экспериментальных исследований, ставящие целью создание более эффективного средства лечения данного заболевания. Материалы и методы. Объектом исследования явились полипренолы, выделенные из пихты сибирской (Abiessibirica Ledeb.). Эксперименты выполнены на 108 аутбредных половозрелых мышах-самцах стока СDI массой тела 28 – 30г (в возрасте 5 недель). Эффект суммы полипренолов на обучаемость и память исследовался в интервале доз – 5, 20, 50, 100, 200 и 500 мг/кг массы тела мыши. В качестве препарата сравнения использовали глиатилин в дозе 90 мг/кг. Экспериментальная модель нарушения когнитивных функций создавалась хроническим (в течение 20 дней) внутрибрюшинным введением скополамина в дозе 2 мг/кг. Нарушение когнитивных функций оценивались по изменениям показателей выработки и воспроизведения условного рефлекса пассивного избегания (УРПИ). Результаты и их обсуждение. Результаты проведенных экспериментов показали, что хроническое введение скополамина вызвало выраженное снижение воспроизводимости УРПИ. Одновременно с этим изучаемая субстанция в дозах 20 и 50 мг/кг. Глиатилин оказывал близкое по характеру действие. Авторы полагают что в развитии антиамнестического эффекта участвуют холинергические рецепторы. Заключение. Приведенное доклиническое исследование показывает, что найдено новое средство с антиамнестическим действием на основе суммы полипренолов Пихты сибирской (Abies sibirica Ledeb.), семейство Сосновые (fam. Pinaceae). Данное средство может быть использовано для лечения заболеваний нейродегенеративных патологических процессов, в частности болезни Альцгеймера.
{"title":"Исследование влияния полипренолов пихты сибирской на обучаемость и память мышей с экспериментальной моделью болезни Альцгеймера","authors":"Николай Иннокентьевич Суслов, Юлия С. Федорова, Максим Л. Коробов","doi":"10.18413/rrpharmacology.10.489","DOIUrl":"https://doi.org/10.18413/rrpharmacology.10.489","url":null,"abstract":"Введение. Болезнь Альцгеймера все чаще становится причиной ранней инвалидизацией и смерти. Попытки создания фармакотерапевтического средства, дающего эффективный результат в лечении этой патологии, до сегодняшнего дня не увенчались успехом. В данной статье приведены результаты экспериментальных исследований, ставящие целью создание более эффективного средства лечения данного заболевания.\u0000Материалы и методы. Объектом исследования явились полипренолы, выделенные из пихты сибирской (Abiessibirica Ledeb.). Эксперименты выполнены на 108 аутбредных половозрелых мышах-самцах стока СDI массой тела 28 – 30г (в возрасте 5 недель). Эффект суммы полипренолов на обучаемость и память исследовался в интервале доз – 5, 20, 50, 100, 200 и 500 мг/кг массы тела мыши. В качестве препарата сравнения использовали глиатилин в дозе 90 мг/кг. Экспериментальная модель нарушения когнитивных функций создавалась хроническим (в течение 20 дней) внутрибрюшинным введением скополамина в дозе 2 мг/кг. Нарушение когнитивных функций оценивались по изменениям показателей выработки и воспроизведения условного рефлекса пассивного избегания (УРПИ).\u0000Результаты и их обсуждение. Результаты проведенных экспериментов показали, что хроническое введение скополамина вызвало выраженное снижение воспроизводимости УРПИ. Одновременно с этим изучаемая субстанция в дозах 20 и 50 мг/кг. Глиатилин оказывал близкое по характеру действие. Авторы полагают что в развитии антиамнестического эффекта участвуют холинергические рецепторы.\u0000Заключение. Приведенное доклиническое исследование показывает, что найдено новое средство с антиамнестическим действием на основе суммы полипренолов Пихты сибирской (Abies sibirica Ledeb.), семейство Сосновые (fam. Pinaceae). Данное средство может быть использовано для лечения заболеваний нейродегенеративных патологических процессов, в частности болезни Альцгеймера.","PeriodicalId":21030,"journal":{"name":"Research Results in Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141927328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-19DOI: 10.18413/rrpharmacology.10.497
P. Galenko-Yaroshevsky, I. Torshin, A. N. Gromov, I. A. Reyer, O. Gromova, Tereza R. Glechyan, Konstantin F. Suzdalev, Andrey V. Zadorozhniy, A. Zelenskaya
Introduction: For effective and safe pharmacotherapy of pain, it is important to evaluate the mechanisms and spectrum of action of non-steroidal anti-inflammatory drugs (NSAIDs), including their effect on the proteom, central effect, as well as pain relieving and anti-inflammatory effects. The aim of the study was to evaluate the complex of differences between the promising candidate-molecule of indole derivative SV-1010 and the well-known NSAIDs. Materials and Methods: Chemoproteomic moduling of pharmacological effects of SV-1010 and NSAID diclofenac, nimesulide and ketorolac on the rat proteom by means of topological analysis of chemographs. Results: The significant differences in the effects of the studied molecules were found for 820 proteins of the rat proteom. SV-1010, to a lesser degree than the other molecules, can inhibit dopamine D1- and D2-type receptors and, at the same time, stimulate the release of dopamine in the neostriatum (EC50 = 27 nM). SV-1010, to a greater extent than the other molecules, can inhibit the GABA conveyor (EC50 = 65 nM) and the NMDA receptors Grin1/Grin2b (IC50 175 nM). SV-1010 can activate Cannabinoid CB2 receptors, inhibit enzymes of leukotriene biosynthesis, CC receptors of pro-inflammatory chemokines and leukotrienes. Conclusion: The chemoreactomic and chemoproteomic profiling of SV-1010 indicated its potential central effect through dopaminergic and GABA-neurotransmission and additional anti-inflammatory mechanisms, which can help increase pain-relieving effects.
{"title":"Chemoproteomic analysis of the promising candidate molecule of the indole derivative with lab code SV-1010 and other non-steroidal anti-inflammatory drugs","authors":"P. Galenko-Yaroshevsky, I. Torshin, A. N. Gromov, I. A. Reyer, O. Gromova, Tereza R. Glechyan, Konstantin F. Suzdalev, Andrey V. Zadorozhniy, A. Zelenskaya","doi":"10.18413/rrpharmacology.10.497","DOIUrl":"https://doi.org/10.18413/rrpharmacology.10.497","url":null,"abstract":"Introduction: For effective and safe pharmacotherapy of pain, it is important to evaluate the mechanisms and spectrum of action of non-steroidal anti-inflammatory drugs (NSAIDs), including their effect on the proteom, central effect, as well as pain relieving and anti-inflammatory effects. The aim of the study was to evaluate the complex of differences between the promising candidate-molecule of indole derivative SV-1010 and the well-known NSAIDs.\u0000Materials and Methods: Chemoproteomic moduling of pharmacological effects of SV-1010 and NSAID diclofenac, nimesulide and ketorolac on the rat proteom by means of topological analysis of chemographs.\u0000Results: The significant differences in the effects of the studied molecules were found for 820 proteins of the rat proteom. SV-1010, to a lesser degree than the other molecules, can inhibit dopamine D1- and D2-type receptors and, at the same time, stimulate the release of dopamine in the neostriatum (EC50 = 27 nM). SV-1010, to a greater extent than the other molecules, can inhibit the GABA conveyor (EC50 = 65 nM) and the NMDA receptors Grin1/Grin2b (IC50 175 nM). SV-1010 can activate Cannabinoid CB2 receptors, inhibit enzymes of leukotriene biosynthesis, CC receptors of pro-inflammatory chemokines and leukotrienes.\u0000Conclusion: The chemoreactomic and chemoproteomic profiling of SV-1010 indicated its potential central effect through dopaminergic and GABA-neurotransmission and additional anti-inflammatory mechanisms, which can help increase pain-relieving effects.","PeriodicalId":21030,"journal":{"name":"Research Results in Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141823940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-12DOI: 10.18413/rrpharmacology.10.453
Viktoria A. Pakina, Evgeniya Z. Iksanova, Evgeniya V. Shih, O. Tumutolova, Karen K. Arutiunian, I V Kargina, K. Blinov, Fedor P. Pilgaev, A. Epishkina, D. Blinov, E. V. Grebenkin, E. Blinova
Introduction: EGFR-activating overexpression or somatic mutations are common in different human cancers. In this regard, the search for promising ways to control the carcinogenic transformation of tumor cells and the progression of malignant tumors expressing EGFR seems to be one of the most promising and developing areas of modern molecular pathology and pharmacology. Material and Methods: An antitumor activity of a novel compound, a pyridine carboxylic acid derivative LHT-17-19, was studied. The molecule was developed and synthesized at the Department of Chemistry, Drug Design and Technology of All-Russian Research Center for Biological Active Compounds Safety (Russia). The study was carried out in cell cultures of stomach cancer (Hs746T, AGS and MKN1) and patient-derived organoid (PDO) model of breast cancer (BC) expressing wild-type EGFR. Results: It was shown that LHT-17-19 induced concentration-dependent cytotoxicity of EGFR-expressing gastric cancer cells of all the aforementioned cultures. Pathomorphological, immunohistochemical and molecular validation of BC organoids derived from ductal breast carcinoma cells of a 68-year-old patient was done. PDOs were established as ER-negative, PR-negative, Her2/neu-negative, EGFR-positive with 35% of the Ki-67 expression index. In addition, the tumor cells translocation was resulted in a loss of ER expression and PDOs molecular pattern conversion towards a more aggressive triple negative type. PDOs incubation with 0.5-60.0 µM LHT-17-19 was accompanied not only by inhibition of their growth and proliferation, but also by significant cytoreduction. Conclusion: Thus, in two-dimensional and three-dimensional tumor cell cultures, the possibility of controlling the oncogenic expression of EGFR with the acridone compound 9-ammonium-3,3-dimethyl-3,4-dihydroacridine-1(2H)-OH L-2-hydroxybutanedivacate (LHT-17-19) was shown.
{"title":"An effective way for targeting EGFR-mediated carcinogenesis: an in vitro study","authors":"Viktoria A. Pakina, Evgeniya Z. Iksanova, Evgeniya V. Shih, O. Tumutolova, Karen K. Arutiunian, I V Kargina, K. Blinov, Fedor P. Pilgaev, A. Epishkina, D. Blinov, E. V. Grebenkin, E. Blinova","doi":"10.18413/rrpharmacology.10.453","DOIUrl":"https://doi.org/10.18413/rrpharmacology.10.453","url":null,"abstract":"Introduction: EGFR-activating overexpression or somatic mutations are common in different human cancers. In this regard, the search for promising ways to control the carcinogenic transformation of tumor cells and the progression of malignant tumors expressing EGFR seems to be one of the most promising and developing areas of modern molecular pathology and pharmacology.\u0000Material and Methods: An antitumor activity of a novel compound, a pyridine carboxylic acid derivative LHT-17-19, was studied. The molecule was developed and synthesized at the Department of Chemistry, Drug Design and Technology of All-Russian Research Center for Biological Active Compounds Safety (Russia). The study was carried out in cell cultures of stomach cancer (Hs746T, AGS and MKN1) and patient-derived organoid (PDO) model of breast cancer (BC) expressing wild-type EGFR.\u0000Results: It was shown that LHT-17-19 induced concentration-dependent cytotoxicity of EGFR-expressing gastric cancer cells of all the aforementioned cultures. Pathomorphological, immunohistochemical and molecular validation of BC organoids derived from ductal breast carcinoma cells of a 68-year-old patient was done. PDOs were established as ER-negative, PR-negative, Her2/neu-negative, EGFR-positive with 35% of the Ki-67 expression index. In addition, the tumor cells translocation was resulted in a loss of ER expression and PDOs molecular pattern conversion towards a more aggressive triple negative type. PDOs incubation with 0.5-60.0 µM LHT-17-19 was accompanied not only by inhibition of their growth and proliferation, but also by significant cytoreduction.\u0000Conclusion: Thus, in two-dimensional and three-dimensional tumor cell cultures, the possibility of controlling the oncogenic expression of EGFR with the acridone compound 9-ammonium-3,3-dimethyl-3,4-dihydroacridine-1(2H)-OH L-2-hydroxybutanedivacate (LHT-17-19) was shown.","PeriodicalId":21030,"journal":{"name":"Research Results in Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141350110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-07DOI: 10.18413/rrpharmacology.10.458
A. A. Chulanova, A. M. Smakhtina, Galina S. Mal, I. Bobyntsev, E. Mishina, M. Y. Smakhtin, Pavel M. Kopeykin, Elena G. Bogomolova
Introduction: It is known that the insertion of D-amino acids into the structure of a peptide molecule can increase its half-life. The pharmacological drug Thymogen was modified with D-alanine from the N- and C-terminus of the molecule. The aim of the investigation was to study the reparative and antioxidant activity of structural analogues of thymogen in toxic liver damage by hydrazine in rats. Materials and Methods: The investigation was conducted on 40 Wistar rats. Acute toxic liver damage was simulated with a single intraperitoneal injection of hydrazine hydrochloride at a dose of 50 mg/kg. Thymogen and its modified D-Ala analogues were administered intraperitoneally in equimolar doses for 5 days after intoxication. The animals were removed from the experiment 12 hours after the final administration of the peptides. Results and Discussion: It has been established the reparative and antioxidant activities of thymogen structural analogues increased in conditions of liver damage with hydrazine. Peptides with the insertion of D-Ala reduced the level of free radical reactions more significantly in comparison with thymogen. All peptides comparably increased catalase activity in blood plasma and liver homogenate. Conclusion: Thymogen analogues modified with D-Ala from the N- and C-terminus of the molecule can be considered as promising pharmacological substances for the development of new hepatoprotective drugs.
简介众所周知，在肽分子结构中加入 D-氨基酸可延长其半衰期。药用药物 Thymogen 在分子的 N 端和 C 端加入了 D-丙氨酸。调查的目的是研究胸腺肽原结构类似物对大鼠肼中毒性肝损伤的修复和抗氧化活性：研究对象为 40 只 Wistar 大鼠。通过腹腔注射 50 毫克/千克剂量的盐酸肼来模拟急性中毒性肝损伤。在中毒后的 5 天内，以等摩尔剂量腹腔注射胸腺肽原及其改良的 D-Ala 类似物。最后一次给药 12 小时后，将动物从实验中移出：已证实胸腺肽结构类似物的修复和抗氧化活性在肼损伤肝脏的情况下会增加。与胸腺肽相比，插入 D-Ala 的肽能更显著地降低自由基反应的水平。所有肽都能显著提高血浆和肝匀浆中过氧化氢酶的活性：结论：在胸腺肽分子的 N 端和 C 端加入 D-Ala 修饰的胸腺肽类似物可被视为具有开发新型保肝药物前景的药理物质。
{"title":"Protective effects of thymogen analogues, modified by D-alanine, in hydrazine liver damage","authors":"A. A. Chulanova, A. M. Smakhtina, Galina S. Mal, I. Bobyntsev, E. Mishina, M. Y. Smakhtin, Pavel M. Kopeykin, Elena G. Bogomolova","doi":"10.18413/rrpharmacology.10.458","DOIUrl":"https://doi.org/10.18413/rrpharmacology.10.458","url":null,"abstract":"Introduction: It is known that the insertion of D-amino acids into the structure of a peptide molecule can increase its half-life. The pharmacological drug Thymogen was modified with D-alanine from the N- and C-terminus of the molecule. The aim of the investigation was to study the reparative and antioxidant activity of structural analogues of thymogen in toxic liver damage by hydrazine in rats.\u0000Materials and Methods: The investigation was conducted on 40 Wistar rats. Acute toxic liver damage was simulated with a single intraperitoneal injection of hydrazine hydrochloride at a dose of 50 mg/kg. Thymogen and its modified D-Ala analogues were administered intraperitoneally in equimolar doses for 5 days after intoxication. The animals were removed from the experiment 12 hours after the final administration of the peptides.\u0000Results and Discussion: It has been established the reparative and antioxidant activities of thymogen structural analogues increased in conditions of liver damage with hydrazine. Peptides with the insertion of D-Ala reduced the level of free radical reactions more significantly in comparison with thymogen. All peptides comparably increased catalase activity in blood plasma and liver homogenate.\u0000Conclusion: Thymogen analogues modified with D-Ala from the N- and C-terminus of the molecule can be considered as promising pharmacological substances for the development of new hepatoprotective drugs.","PeriodicalId":21030,"journal":{"name":"Research Results in Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141373958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-18DOI: 10.18413/rrpharmacology.10.437
V. N. Perfilova, E. A. Muzyko, K. Y. Tikhaeva, M. V. Kustova, Anna V. Mukhina, Anna Yu. Fokina, Evgeniya I. Zhuravleva, Tatyana D. Lenskaya
Introduction: The problem of infertility treatment currently has medical, socio-demographic and economic significance. Progress in the field of reproductive technologies has improved the situation, but the issue has not yet been completely resolved. Infertility is diagnosed in 8-12% of couples of reproductive age; in Russia this figure exceeds 15%, and, according to WHO, it is a critical level. It is known that immunological factors can disrupt the reproductive process at the stages of folliculogenesis, ovulation, and implantation. These include vascular endothelial growth factor (VEGF), tumor necrosis factor-alpha (TNF-α) and others. The aim of this study: to assess the levels of VEGF and TNF-α in women with different outcomes of hormonal stimulation of the ovaries during the treatment of infertility using ART methods. Materials and methods: Based on voluntary informed consent, a simple open comparative randomized study was conducted with the participation of 71 women in the Volgograd region undergoing infertility treatment using ART methods. Inclusion criteria were the age up to 42 years inclusive and the anti-Mullerian hormone level over 1.2 ng/mL. Before ovulation stimulation with gonadotropins, data on ovarian reserve parameters were collected. Based on the results of stimulation, women were divided into 3 groups: Group 1 – with high and normal ovarian response – 10 or more oocytes were obtained (control group); Group 2 – with suboptimal ovarian response – 5-9 oocytes received; Group 3 – with a poor response – 4 or less oocytes were received. After venipuncture, which was performed in preparation for standard anesthesia, VEGF and TNF-α were quantitatively determined in the blood plasma using an enzyme-linked immunosorbent assay. Results: The study results showed a statistically significant difference in VEGF levels in follicular fluid and serum between the groups of women with high/normal, suboptimal and poor ovarian response to gonadotropin stimulation. A higher level of this marker was observed in the serum of patients with high/normal and poor response – 48.15±4.23 and 41.29±8.26 pg/mL, respectively, while in women with a suboptimal response a lower VEGF level was determined –29.19±3.41 pg/mL. The levels of VEGF in the follicular fluid of women included in the high/normal, suboptimal and poor response groups were 35.95±3.20; 27.42±2.53 and 41.22±3.23 pg/mL, respectively (Table 1). As for TNF-α, its serum level in women with a high and normal response was lower than in the patients with a suboptimal response. In the follicular fluid of patients of group 3, there was a higher level of TNF-α, compared to groups 1 and 2, where the indicator was almost the same. However, the difference was not statistically significant. Conclusions: Thus, VEGF is directly involved in the mechanisms of regulation of oocyte maturation and can be not only a marker of poor ovarian response, but also a predictor of unsatisfactory results of ovarian stimulation in t
{"title":"Assessment of VEGF and TNF-alpha levels in patients with an unexpectedly poor and suboptimal response during the treatment of infertility using ART methods","authors":"V. N. Perfilova, E. A. Muzyko, K. Y. Tikhaeva, M. V. Kustova, Anna V. Mukhina, Anna Yu. Fokina, Evgeniya I. Zhuravleva, Tatyana D. Lenskaya","doi":"10.18413/rrpharmacology.10.437","DOIUrl":"https://doi.org/10.18413/rrpharmacology.10.437","url":null,"abstract":"Introduction: The problem of infertility treatment currently has medical, socio-demographic and economic significance. Progress in the field of reproductive technologies has improved the situation, but the issue has not yet been completely resolved. Infertility is diagnosed in 8-12% of couples of reproductive age; in Russia this figure exceeds 15%, and, according to WHO, it is a critical level. It is known that immunological factors can disrupt the reproductive process at the stages of folliculogenesis, ovulation, and implantation. These include vascular endothelial growth factor (VEGF), tumor necrosis factor-alpha (TNF-α) and others. The aim of this study: to assess the levels of VEGF and TNF-α in women with different outcomes of hormonal stimulation of the ovaries during the treatment of infertility using ART methods.\u0000Materials and methods: Based on voluntary informed consent, a simple open comparative randomized study was conducted with the participation of 71 women in the Volgograd region undergoing infertility treatment using ART methods. Inclusion criteria were the age up to 42 years inclusive and the anti-Mullerian hormone level over 1.2 ng/mL. Before ovulation stimulation with gonadotropins, data on ovarian reserve parameters were collected. Based on the results of stimulation, women were divided into 3 groups: Group 1 – with high and normal ovarian response – 10 or more oocytes were obtained (control group); Group 2 – with suboptimal ovarian response – 5-9 oocytes received; Group 3 – with a poor response – 4 or less oocytes were received. After venipuncture, which was performed in preparation for standard anesthesia, VEGF and TNF-α were quantitatively determined in the blood plasma using an enzyme-linked immunosorbent assay.\u0000Results: The study results showed a statistically significant difference in VEGF levels in follicular fluid and serum between the groups of women with high/normal, suboptimal and poor ovarian response to gonadotropin stimulation. A higher level of this marker was observed in the serum of patients with high/normal and poor response – 48.15±4.23 and 41.29±8.26 pg/mL, respectively, while in women with a suboptimal response a lower VEGF level was determined –29.19±3.41 pg/mL. The levels of VEGF in the follicular fluid of women included in the high/normal, suboptimal and poor response groups were 35.95±3.20; 27.42±2.53 and 41.22±3.23 pg/mL, respectively (Table 1). As for TNF-α, its serum level in women with a high and normal response was lower than in the patients with a suboptimal response. In the follicular fluid of patients of group 3, there was a higher level of TNF-α, compared to groups 1 and 2, where the indicator was almost the same. However, the difference was not statistically significant.\u0000Conclusions: Thus, VEGF is directly involved in the mechanisms of regulation of oocyte maturation and can be not only a marker of poor ovarian response, but also a predictor of unsatisfactory results of ovarian stimulation in t","PeriodicalId":21030,"journal":{"name":"Research Results in Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140689849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-13DOI: 10.18413/rrpharmacology.10.443
P. Galenko-Yaroshevsky, O. V. Shelemekh, V. Popkov, Andrey V. Zadorozhniy, Irina B. Nektarevskaya, Natalia D. Bunyatyan, Roman A. Murashko, S. Lebedeva, A. Zelenskaya, Aleksandr V. Uvarov, O. N. Gulevskaya, Lusine O. Alukhanyan, Tereza R. Glechyan, Alina V. Sergeeva, Alina V. Kornetskaya, Nikolay E. Korovaykin
Introduction: Although nonsteroidal anti-inflammatory drugs (NSAIDs) have a clear therapeutic benefit, they can also have serious side effects. The most serious of these is the ulcerogenic effect, which can result in a decline in the quality of life or even death. This calls for the search for and creation of novel compounds with potent anti-inflammatory properties that do not have adverse effects on the digestive system. The aim of the study was to investigate the anti-inflammatory, analgesic, ulcerogenic and anti-ulcerogenic activities of N-isopropenylimidazole zinc complex derivative. Materials and Methods: Experiments in rats and mice were used to determine the median lethal dose LD50 for N-isopropenylimidazole zinc complex derivative (laboratory name Pilim-1). Experimental models of acute exudative inflammation and chronic proliferative inflammation were used. In the first case, the inflammation was induced by sub-plantar injection of carrageenan and a complete Freund’s adjuvant into the hind paw of rats. In the second case, the inflammation was induced by the implantation of a sterile cotton pellet (“cotton pellet–induced granuloma”) under the skin of rats. Acute and tonic pain, visceral, and somatic deep pain were simulated using the formalin test in rats and the acetic acid-induced writhing test in mice, respectively. The ulcerogenic and anti-ulcerogenic effects of Pilim-1 were studied in rat experiments. Pilim-1 and the reference drugs diclofenac, indomethacin and famotidine were administered intragastrically. Results and Discussion: Pilim-1 showed marked anti-inflammatory and analgesic effects, demonstrated less toxicity than diclofenac and indomethacin, while its activity is comparable to diclofenac and superior to indomethacin. Its therapeutic index is higher than that of indomethacin and diclofenac and, in contrast, it essentially has no ulcerogenic effect and exhibits stronger anti-ulcerogenic activity than famotidine. It appears that the anti-inflammatory, analgesic, and gastroprotective properties of Pilim-1 are largely due to its antioxidant and antihypoxic properties, its inhibitory effects on cyclooxygenase and 5-lipoxygenase, and the presence of imidazole and zinc in its structure, both of which have a wide range of biological activity. Conclusion: Pilim-1 can be recommended for further preclinical studies due to its relatively low (practically absent) ulcerative activity, strong anti-inflammatory, analgesic, and anti-ulcerative effects, greater therapeutic index, and less acute toxicity in comparison with diclofenac and indomethacin.
{"title":"Study of the anti-inflammatory, analgesic, ulcerogenic and anti-ulcerogenic activity of N-isopropenylimidazole zinc complex derivative","authors":"P. Galenko-Yaroshevsky, O. V. Shelemekh, V. Popkov, Andrey V. Zadorozhniy, Irina B. Nektarevskaya, Natalia D. Bunyatyan, Roman A. Murashko, S. Lebedeva, A. Zelenskaya, Aleksandr V. Uvarov, O. N. Gulevskaya, Lusine O. Alukhanyan, Tereza R. Glechyan, Alina V. Sergeeva, Alina V. Kornetskaya, Nikolay E. Korovaykin","doi":"10.18413/rrpharmacology.10.443","DOIUrl":"https://doi.org/10.18413/rrpharmacology.10.443","url":null,"abstract":"Introduction: Although nonsteroidal anti-inflammatory drugs (NSAIDs) have a clear therapeutic benefit, they can also have serious side effects. The most serious of these is the ulcerogenic effect, which can result in a decline in the quality of life or even death. This calls for the search for and creation of novel compounds with potent anti-inflammatory properties that do not have adverse effects on the digestive system. The aim of the study was to investigate the anti-inflammatory, analgesic, ulcerogenic and anti-ulcerogenic activities of N-isopropenylimidazole zinc complex derivative.\u0000Materials and Methods: Experiments in rats and mice were used to determine the median lethal dose LD50 for N-isopropenylimidazole zinc complex derivative (laboratory name Pilim-1). Experimental models of acute exudative inflammation and chronic proliferative inflammation were used. In the first case, the inflammation was induced by sub-plantar injection of carrageenan and a complete Freund’s adjuvant into the hind paw of rats. In the second case, the inflammation was induced by the implantation of a sterile cotton pellet (“cotton pellet–induced granuloma”) under the skin of rats. Acute and tonic pain, visceral, and somatic deep pain were simulated using the formalin test in rats and the acetic acid-induced writhing test in mice, respectively. The ulcerogenic and anti-ulcerogenic effects of Pilim-1 were studied in rat experiments. Pilim-1 and the reference drugs diclofenac, indomethacin and famotidine were administered intragastrically.\u0000Results and Discussion: Pilim-1 showed marked anti-inflammatory and analgesic effects, demonstrated less toxicity than diclofenac and indomethacin, while its activity is comparable to diclofenac and superior to indomethacin. Its therapeutic index is higher than that of indomethacin and diclofenac and, in contrast, it essentially has no ulcerogenic effect and exhibits stronger anti-ulcerogenic activity than famotidine. It appears that the anti-inflammatory, analgesic, and gastroprotective properties of Pilim-1 are largely due to its antioxidant and antihypoxic properties, its inhibitory effects on cyclooxygenase and 5-lipoxygenase, and the presence of imidazole and zinc in its structure, both of which have a wide range of biological activity.\u0000Conclusion: Pilim-1 can be recommended for further preclinical studies due to its relatively low (practically absent) ulcerative activity, strong anti-inflammatory, analgesic, and anti-ulcerative effects, greater therapeutic index, and less acute toxicity in comparison with diclofenac and indomethacin.","PeriodicalId":21030,"journal":{"name":"Research Results in Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139781638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-13DOI: 10.18413/rrpharmacology.10.443
P. Galenko-Yaroshevsky, O. V. Shelemekh, V. Popkov, Andrey V. Zadorozhniy, Irina B. Nektarevskaya, Natalia D. Bunyatyan, Roman A. Murashko, S. Lebedeva, A. Zelenskaya, Aleksandr V. Uvarov, O. N. Gulevskaya, Lusine O. Alukhanyan, Tereza R. Glechyan, Alina V. Sergeeva, Alina V. Kornetskaya, Nikolay E. Korovaykin
Introduction: Although nonsteroidal anti-inflammatory drugs (NSAIDs) have a clear therapeutic benefit, they can also have serious side effects. The most serious of these is the ulcerogenic effect, which can result in a decline in the quality of life or even death. This calls for the search for and creation of novel compounds with potent anti-inflammatory properties that do not have adverse effects on the digestive system. The aim of the study was to investigate the anti-inflammatory, analgesic, ulcerogenic and anti-ulcerogenic activities of N-isopropenylimidazole zinc complex derivative. Materials and Methods: Experiments in rats and mice were used to determine the median lethal dose LD50 for N-isopropenylimidazole zinc complex derivative (laboratory name Pilim-1). Experimental models of acute exudative inflammation and chronic proliferative inflammation were used. In the first case, the inflammation was induced by sub-plantar injection of carrageenan and a complete Freund’s adjuvant into the hind paw of rats. In the second case, the inflammation was induced by the implantation of a sterile cotton pellet (“cotton pellet–induced granuloma”) under the skin of rats. Acute and tonic pain, visceral, and somatic deep pain were simulated using the formalin test in rats and the acetic acid-induced writhing test in mice, respectively. The ulcerogenic and anti-ulcerogenic effects of Pilim-1 were studied in rat experiments. Pilim-1 and the reference drugs diclofenac, indomethacin and famotidine were administered intragastrically. Results and Discussion: Pilim-1 showed marked anti-inflammatory and analgesic effects, demonstrated less toxicity than diclofenac and indomethacin, while its activity is comparable to diclofenac and superior to indomethacin. Its therapeutic index is higher than that of indomethacin and diclofenac and, in contrast, it essentially has no ulcerogenic effect and exhibits stronger anti-ulcerogenic activity than famotidine. It appears that the anti-inflammatory, analgesic, and gastroprotective properties of Pilim-1 are largely due to its antioxidant and antihypoxic properties, its inhibitory effects on cyclooxygenase and 5-lipoxygenase, and the presence of imidazole and zinc in its structure, both of which have a wide range of biological activity. Conclusion: Pilim-1 can be recommended for further preclinical studies due to its relatively low (practically absent) ulcerative activity, strong anti-inflammatory, analgesic, and anti-ulcerative effects, greater therapeutic index, and less acute toxicity in comparison with diclofenac and indomethacin.
{"title":"Study of the anti-inflammatory, analgesic, ulcerogenic and anti-ulcerogenic activity of N-isopropenylimidazole zinc complex derivative","authors":"P. Galenko-Yaroshevsky, O. V. Shelemekh, V. Popkov, Andrey V. Zadorozhniy, Irina B. Nektarevskaya, Natalia D. Bunyatyan, Roman A. Murashko, S. Lebedeva, A. Zelenskaya, Aleksandr V. Uvarov, O. N. Gulevskaya, Lusine O. Alukhanyan, Tereza R. Glechyan, Alina V. Sergeeva, Alina V. Kornetskaya, Nikolay E. Korovaykin","doi":"10.18413/rrpharmacology.10.443","DOIUrl":"https://doi.org/10.18413/rrpharmacology.10.443","url":null,"abstract":"Introduction: Although nonsteroidal anti-inflammatory drugs (NSAIDs) have a clear therapeutic benefit, they can also have serious side effects. The most serious of these is the ulcerogenic effect, which can result in a decline in the quality of life or even death. This calls for the search for and creation of novel compounds with potent anti-inflammatory properties that do not have adverse effects on the digestive system. The aim of the study was to investigate the anti-inflammatory, analgesic, ulcerogenic and anti-ulcerogenic activities of N-isopropenylimidazole zinc complex derivative.\u0000Materials and Methods: Experiments in rats and mice were used to determine the median lethal dose LD50 for N-isopropenylimidazole zinc complex derivative (laboratory name Pilim-1). Experimental models of acute exudative inflammation and chronic proliferative inflammation were used. In the first case, the inflammation was induced by sub-plantar injection of carrageenan and a complete Freund’s adjuvant into the hind paw of rats. In the second case, the inflammation was induced by the implantation of a sterile cotton pellet (“cotton pellet–induced granuloma”) under the skin of rats. Acute and tonic pain, visceral, and somatic deep pain were simulated using the formalin test in rats and the acetic acid-induced writhing test in mice, respectively. The ulcerogenic and anti-ulcerogenic effects of Pilim-1 were studied in rat experiments. Pilim-1 and the reference drugs diclofenac, indomethacin and famotidine were administered intragastrically.\u0000Results and Discussion: Pilim-1 showed marked anti-inflammatory and analgesic effects, demonstrated less toxicity than diclofenac and indomethacin, while its activity is comparable to diclofenac and superior to indomethacin. Its therapeutic index is higher than that of indomethacin and diclofenac and, in contrast, it essentially has no ulcerogenic effect and exhibits stronger anti-ulcerogenic activity than famotidine. It appears that the anti-inflammatory, analgesic, and gastroprotective properties of Pilim-1 are largely due to its antioxidant and antihypoxic properties, its inhibitory effects on cyclooxygenase and 5-lipoxygenase, and the presence of imidazole and zinc in its structure, both of which have a wide range of biological activity.\u0000Conclusion: Pilim-1 can be recommended for further preclinical studies due to its relatively low (practically absent) ulcerative activity, strong anti-inflammatory, analgesic, and anti-ulcerative effects, greater therapeutic index, and less acute toxicity in comparison with diclofenac and indomethacin.","PeriodicalId":21030,"journal":{"name":"Research Results in Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139841485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-09DOI: 10.18413/rrpharmacology.10.436
D. Y. Perfileva, Alexander G. Miroshnichenko, E. S. Kulikov, V. Y. Perfilev, V. Boykov, S. Nesterovich
Introduction: One of the frequent causes of re-hospitalization is infectious complications due to previous colonization of patient loci by microorganisms circulating in the hospital environment. In the conditions of real clinical practice among hospital-acquired infections (HAI), it is advisable to distinguish a special group of diseases – infections associated with previous hospitalization (IPAH). Of particular scientific interest is the study of the antibiotic resistance profile of IPAH pathogens in order to determine the further strategy of empirical antibiotic therapy. Materials and Methods: A two-center descriptive study was conducted in Tomsk region. We analyzed 170 cases of IPAH according to the medical records of patients receiving medical care in inpatient settings (form N 003/u) in the period from 2019 to 2023. Identification of microorganisms was carried out by classical bacteriological method. Results and Discussion: Gram-negative bacteria (95.3%) predominated in the etiology of pneumonia associated with prior hospitalization. Among Gram-negative microorganisms, the most frequent were K. pneumoniae, P. aeroginosa and K. oxytoca. Representatives of the families Enterobacteriaceae (48.2%), Staphylococcaceae (28.9%) and Enterococcaceae (10.8%) predominated in the etiology of surgical infection associated with previous hospitalization. In the species structure, the key pathogens were K. pneumoniae, S. aureus and E. coli. IPAH pathogens were characterized by an unfavorable resistance profile. Conclusion: Despite the fact that the etiological structure and antibiotic resistance profile of IPAH are similar to those of classical nosocomial infections, IPAH has important features that should certainly be taken into account when organizing medical care for this cohort of patients.
{"title":"Antibiotic resistance of infectious agents associated with prior hospitalization","authors":"D. Y. Perfileva, Alexander G. Miroshnichenko, E. S. Kulikov, V. Y. Perfilev, V. Boykov, S. Nesterovich","doi":"10.18413/rrpharmacology.10.436","DOIUrl":"https://doi.org/10.18413/rrpharmacology.10.436","url":null,"abstract":"\u0000Introduction: One of the frequent causes of re-hospitalization is infectious complications due to previous colonization of patient loci by microorganisms circulating in the hospital environment. In the conditions of real clinical practice among hospital-acquired infections (HAI), it is advisable to distinguish a special group of diseases – infections associated with previous hospitalization (IPAH). Of particular scientific interest is the study of the antibiotic resistance profile of IPAH pathogens in order to determine the further strategy of empirical antibiotic therapy.\u0000\u0000\u0000Materials and Methods: A two-center descriptive study was conducted in Tomsk region. We analyzed 170 cases of IPAH according to the medical records of patients receiving medical care in inpatient settings (form N 003/u) in the period from 2019 to 2023. Identification of microorganisms was carried out by classical bacteriological method. \u0000\u0000\u0000Results and Discussion: Gram-negative bacteria (95.3%) predominated in the etiology of pneumonia associated with prior hospitalization. Among Gram-negative microorganisms, the most frequent were K. pneumoniae, P. aeroginosa and K. oxytoca. Representatives of the families Enterobacteriaceae (48.2%), Staphylococcaceae (28.9%) and Enterococcaceae (10.8%) predominated in the etiology of surgical infection associated with previous hospitalization. In the species structure, the key pathogens were K. pneumoniae, S. aureus and E. coli. IPAH pathogens were characterized by an unfavorable resistance profile. \u0000\u0000\u0000Conclusion: Despite the fact that the etiological structure and antibiotic resistance profile of IPAH are similar to those of classical nosocomial infections, IPAH has important features that should certainly be taken into account when organizing medical care for this cohort of patients. \u0000","PeriodicalId":21030,"journal":{"name":"Research Results in Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139849835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-09DOI: 10.18413/rrpharmacology.10.436
D. Y. Perfileva, Alexander G. Miroshnichenko, E. S. Kulikov, V. Y. Perfilev, V. Boykov, S. Nesterovich
Introduction: One of the frequent causes of re-hospitalization is infectious complications due to previous colonization of patient loci by microorganisms circulating in the hospital environment. In the conditions of real clinical practice among hospital-acquired infections (HAI), it is advisable to distinguish a special group of diseases – infections associated with previous hospitalization (IPAH). Of particular scientific interest is the study of the antibiotic resistance profile of IPAH pathogens in order to determine the further strategy of empirical antibiotic therapy. Materials and Methods: A two-center descriptive study was conducted in Tomsk region. We analyzed 170 cases of IPAH according to the medical records of patients receiving medical care in inpatient settings (form N 003/u) in the period from 2019 to 2023. Identification of microorganisms was carried out by classical bacteriological method. Results and Discussion: Gram-negative bacteria (95.3%) predominated in the etiology of pneumonia associated with prior hospitalization. Among Gram-negative microorganisms, the most frequent were K. pneumoniae, P. aeroginosa and K. oxytoca. Representatives of the families Enterobacteriaceae (48.2%), Staphylococcaceae (28.9%) and Enterococcaceae (10.8%) predominated in the etiology of surgical infection associated with previous hospitalization. In the species structure, the key pathogens were K. pneumoniae, S. aureus and E. coli. IPAH pathogens were characterized by an unfavorable resistance profile. Conclusion: Despite the fact that the etiological structure and antibiotic resistance profile of IPAH are similar to those of classical nosocomial infections, IPAH has important features that should certainly be taken into account when organizing medical care for this cohort of patients.
{"title":"Antibiotic resistance of infectious agents associated with prior hospitalization","authors":"D. Y. Perfileva, Alexander G. Miroshnichenko, E. S. Kulikov, V. Y. Perfilev, V. Boykov, S. Nesterovich","doi":"10.18413/rrpharmacology.10.436","DOIUrl":"https://doi.org/10.18413/rrpharmacology.10.436","url":null,"abstract":"\u0000Introduction: One of the frequent causes of re-hospitalization is infectious complications due to previous colonization of patient loci by microorganisms circulating in the hospital environment. In the conditions of real clinical practice among hospital-acquired infections (HAI), it is advisable to distinguish a special group of diseases – infections associated with previous hospitalization (IPAH). Of particular scientific interest is the study of the antibiotic resistance profile of IPAH pathogens in order to determine the further strategy of empirical antibiotic therapy.\u0000\u0000\u0000Materials and Methods: A two-center descriptive study was conducted in Tomsk region. We analyzed 170 cases of IPAH according to the medical records of patients receiving medical care in inpatient settings (form N 003/u) in the period from 2019 to 2023. Identification of microorganisms was carried out by classical bacteriological method. \u0000\u0000\u0000Results and Discussion: Gram-negative bacteria (95.3%) predominated in the etiology of pneumonia associated with prior hospitalization. Among Gram-negative microorganisms, the most frequent were K. pneumoniae, P. aeroginosa and K. oxytoca. Representatives of the families Enterobacteriaceae (48.2%), Staphylococcaceae (28.9%) and Enterococcaceae (10.8%) predominated in the etiology of surgical infection associated with previous hospitalization. In the species structure, the key pathogens were K. pneumoniae, S. aureus and E. coli. IPAH pathogens were characterized by an unfavorable resistance profile. \u0000\u0000\u0000Conclusion: Despite the fact that the etiological structure and antibiotic resistance profile of IPAH are similar to those of classical nosocomial infections, IPAH has important features that should certainly be taken into account when organizing medical care for this cohort of patients. \u0000","PeriodicalId":21030,"journal":{"name":"Research Results in Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139789764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-30DOI: 10.18413/rrpharmacology.10.398
Y. Belousova, A. M. Tynterova, V. Rafalskiy
Introduction: The problem of asthenic disorders is determined by their high prevalence, the lack of diagnostic criteria and recommendations for therapy, and the absence of unified principles of coding in ICD-10. The aim of the study was to evaluate the current practice of neurologists and physicians of the Russian Federation (RF) in the diagnosis and pharmacotherapy of asthenic syndrome (AS). Materials and Methods: An anonymous author-developed questionnaire survey of physicians on the issues of diagnostics and treatment of asthenia was conducted on the Google forms platform. Results: Total 238 specialists from 23 regions of the RF, 62.5% of neurologists and 37.5% of physicians, took part in the survey. Women suffer from AS more often than men. AS is most frequently verified at the age of 40-60 (65.5%) and under 40 (39.6%). Doctors use the codes G90.8 – other disorders of the autonomic nervous system – and I67.8 – other specified cerebral vascular lesions. The main causes of AS are affective disorders in 67.2% of patients and infectious diseases in 70% of patients. Almost 67% of doctors use anxiety and depression assessment scale, and only 13% of respondents use MFI-20 scale. The choice of therapy depended on the psychopathologic syndrome in 73.9% of cases. Most doctors favored nootropic drugs, metabolic action drugs, and B vitamins. Conclusion: The current medical practice of diagnosing and treating AS was studied and informative data were obtained with regard to understanding the clinical-typological structure and nosological affiliation of AS. The results demonstrate the expediency of developing a unified algorithm for the management of patients with various manifestations of AS.
{"title":"Modern doctors' view on the problem of diagnostics and treatment of asthenic syndrome in different regions of the Russian Federation","authors":"Y. Belousova, A. M. Tynterova, V. Rafalskiy","doi":"10.18413/rrpharmacology.10.398","DOIUrl":"https://doi.org/10.18413/rrpharmacology.10.398","url":null,"abstract":"Introduction: The problem of asthenic disorders is determined by their high prevalence, the lack of diagnostic criteria and recommendations for therapy, and the absence of unified principles of coding in ICD-10. The aim of the study was to evaluate the current practice of neurologists and physicians of the Russian Federation (RF) in the diagnosis and pharmacotherapy of asthenic syndrome (AS).\u0000Materials and Methods: An anonymous author-developed questionnaire survey of physicians on the issues of diagnostics and treatment of asthenia was conducted on the Google forms platform.\u0000Results: Total 238 specialists from 23 regions of the RF, 62.5% of neurologists and 37.5% of physicians, took part in the survey. Women suffer from AS more often than men. AS is most frequently verified at the age of 40-60 (65.5%) and under 40 (39.6%). Doctors use the codes G90.8 – other disorders of the autonomic nervous system – and I67.8 – other specified cerebral vascular lesions. The main causes of AS are affective disorders in 67.2% of patients and infectious diseases in 70% of patients. Almost 67% of doctors use anxiety and depression assessment scale, and only 13% of respondents use MFI-20 scale. The choice of therapy depended on the psychopathologic syndrome in 73.9% of cases. Most doctors favored nootropic drugs, metabolic action drugs, and B vitamins.\u0000Conclusion: The current medical practice of diagnosing and treating AS was studied and informative data were obtained with regard to understanding the clinical-typological structure and nosological affiliation of AS. The results demonstrate the expediency of developing a unified algorithm for the management of patients with various manifestations of AS.","PeriodicalId":21030,"journal":{"name":"Research Results in Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140484663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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