Pub Date : 2017-03-31DOI: 10.25081/RIB.2017.V8.3594
A. Dhyani, Ritu Gururani, S. Selim, P. Adhikari, Avinash Sharma, V. Pande, A. Pandey
Enzymes from thermophilic bacteria have received great attention for their potential applications in various industrial sectors. The present study deals with the production of five thermozymes (amylase, lipase, xylanase, protease and cellulase) from 10 thermophilic bacterial species, originally isolated from two hot springs namely Soldhar and Ringigad in Uttarakhand Himalaya, India. The bacterial isolate GBPI_25 produced maximum amylase (1217.86 U/ml) at 45 °C and 5 pH, GBPI 3 produced maximum lipase (22.59 U/ml) at 65 °C and 9 pH, GBPI_25 produced maximum xylanase (98.07 U/ml) at45 °C and 9 pH, GBPI_35 produced maximum protease (16.66 U/ml) at 55 °C and 9 pH, and GBPI 4 produced maximum cellulose (108.68 U/ml) at 45 °C and 5 pH. Crude enzyme preparations showed thermal and pH activities at broad temperature and pH range between 10-100 °C and 3-11 pH, respectively, with different temperature and pH optima. Amylase, xylanase and cellulase showed maximum activity at 50 °C while lipase and protease showed higher activity at 40 and 60 °C, respectively. Enzyme activity at wide temperature range-cellulase and protease from 10-100 °C, amylase and xylanasefrom10-90 °C, and lipase activity from 10-80 °C were the remarkable records from this study. Similarly, pH range for amylase and lipase activity was recorded from 4-11, for xylanase from 3-9, and for protease and cellulase from 3-10. All the thermozymes showed maximum stability at 40 °C and pH 5 except cellulase that showed higher stability at40 °C and neutral pH.
从嗜热细菌中提取的酶因其在各个工业领域的潜在应用而受到广泛关注。本研究从10种嗜热细菌中分离出5种热酶(淀粉酶、脂肪酶、木聚糖酶、蛋白酶和纤维素酶)，这些细菌最初是从印度北阿坎德邦喜马拉雅地区的Soldhar和Ringigad两个温泉中分离出来的。细菌隔离GBPI_25产生最大淀粉酶(1217.86 U /毫升)45°C和5 pH值,GBPI 3产生最大脂肪酶(22.59 U /毫升)在65°C和9 pH值,GBPI_25产生最大的木聚糖酶(98.07 U /毫升)at45°C和9 pH值,GBPI_35产生最大蛋白酶(16.66 U /毫升)55°C和9 pH值,产生最大纤维素和GBPI 4 (108.68 U /毫升)45°C和5博士粗酶制剂显示温度和pH值活动在广泛的温度和pH值范围在10 - 100°C和3-11 pH值,分别不同的温度和pH值。淀粉酶、木聚糖酶和纤维素酶在50℃时活性最高，脂肪酶和蛋白酶在40℃和60℃时活性最高。酶在宽温度范围内的活性，纤维素酶和蛋白酶在10-100℃，淀粉酶和木聚糖酶在10-90℃，脂肪酶在10-80℃的活性是本研究的显著记录。同样，淀粉酶和脂肪酶活性的pH范围为4-11，木聚糖酶活性的pH范围为3-9，蛋白酶和纤维素酶活性的pH范围为3-10。除纤维素酶在40°C和pH为中性时表现出较高的稳定性外，所有热酶在40°C和pH为5时表现出最大的稳定性。
{"title":"PRODUCTION OF INDUSTRIALLY IMPORTANT ENZYMES BY THERMOBACILLI ISOLATED FROM HOT SPRINGS OF INDIA","authors":"A. Dhyani, Ritu Gururani, S. Selim, P. Adhikari, Avinash Sharma, V. Pande, A. Pandey","doi":"10.25081/RIB.2017.V8.3594","DOIUrl":"https://doi.org/10.25081/RIB.2017.V8.3594","url":null,"abstract":"Enzymes from thermophilic bacteria have received great attention for their potential applications in various industrial sectors. The present study deals with the production of five thermozymes (amylase, lipase, xylanase, protease and cellulase) from 10 thermophilic bacterial species, originally isolated from two hot springs namely Soldhar and Ringigad in Uttarakhand Himalaya, India. The bacterial isolate GBPI_25 produced maximum amylase (1217.86 U/ml) at 45 °C and 5 pH, GBPI 3 produced maximum lipase (22.59 U/ml) at 65 °C and 9 pH, GBPI_25 produced maximum xylanase (98.07 U/ml) at45 °C and 9 pH, GBPI_35 produced maximum protease (16.66 U/ml) at 55 °C and 9 pH, and GBPI 4 produced maximum cellulose (108.68 U/ml) at 45 °C and 5 pH. Crude enzyme preparations showed thermal and pH activities at broad temperature and pH range between 10-100 °C and 3-11 pH, respectively, with different temperature and pH optima. Amylase, xylanase and cellulase showed maximum activity at 50 °C while lipase and protease showed higher activity at 40 and 60 °C, respectively. Enzyme activity at wide temperature range-cellulase and protease from 10-100 °C, amylase and xylanasefrom10-90 °C, and lipase activity from 10-80 °C were the remarkable records from this study. Similarly, pH range for amylase and lipase activity was recorded from 4-11, for xylanase from 3-9, and for protease and cellulase from 3-10. All the thermozymes showed maximum stability at 40 °C and pH 5 except cellulase that showed higher stability at40 °C and neutral pH.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"15 1","pages":"19-28"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82430894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-31DOI: 10.19071/RIB.2017.V8.3201
D. Chitra, B. Chinthapalli, G. Padmaja
A comparison of protein profiles of leaves during different stages of shoot and callus induction showed similarities as well as differences in the expression of proteins. A protein of 39 kDa was expressed in low levels in leaf explants and increased in intensity during induction of shoot organogenesis in both the cultivars. Analysis of protein patterns during organogenesis and callus proliferation from leaves by two dimensional gel analysis revealed the separation of 39 kDa protein into four spots during organogenesis with pI values ranging from 4.2-5.8. However, the isoforms of 39 kDa protein with pI values of 4.2 and 5.8 were highly expressed in callus of M-5 cultivar in contrast to S-36 cultivar where only one isoform with pI value of 4.2 was detectable. The analysis of protein synthesis in different stages of development in the cultures may acts as markers to differentiate the group of specific isoforms.
{"title":"Protein difference among the leaf explants determined for shoot regeneration and callus growth in Mulberry (Morus indica L.)","authors":"D. Chitra, B. Chinthapalli, G. Padmaja","doi":"10.19071/RIB.2017.V8.3201","DOIUrl":"https://doi.org/10.19071/RIB.2017.V8.3201","url":null,"abstract":"A comparison of protein profiles of leaves during different stages of shoot and callus induction showed similarities as well as differences in the expression of proteins. A protein of 39 kDa was expressed in low levels in leaf explants and increased in intensity during induction of shoot organogenesis in both the cultivars. Analysis of protein patterns during organogenesis and callus proliferation from leaves by two dimensional gel analysis revealed the separation of 39 kDa protein into four spots during organogenesis with pI values ranging from 4.2-5.8. However, the isoforms of 39 kDa protein with pI values of 4.2 and 5.8 were highly expressed in callus of M-5 cultivar in contrast to S-36 cultivar where only one isoform with pI value of 4.2 was detectable. The analysis of protein synthesis in different stages of development in the cultures may acts as markers to differentiate the group of specific isoforms.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"48 1","pages":"01-11"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90674521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-19DOI: 10.19071/RIB.2016.V7.3093
M. Malahubban, Zakry Fitri Ab Aziz
To evaluate the effects of ground leaf of Misai kucing ( Orthosiphon stamineus ) as a dietary supplement on serum biochemical parameters and liver morphology. One hundred and sixty one-day old male broiler chickens (n=160) were distributed into four treatment groups, with five replicates of eight birds in each group: the control group (diet without additives); the group dietary treatments, Diet OS2 (Basal diet + 2g/kg O. stamineus ); Diet OS4 (Basal diet + 4g/kg O. stamineus ) and Diet OS8 (Basal diet + 8g/kg O. stamineus ). After 42 days, 40 birds were randomly selected for serum biochemical profile analysis involving pancreatic, renal, and hepatic functions (urea, sodium, potassium, chlorine, aspartate transaminase (AST), alkaline transaminase (ALT), alkaline phosphatase (ALP), glucose, cholesterol, triglycerides, total protein, albumin, and globulins). Present study found that serum levels of cholesterol, triglycerides, urea, AST, ALT and ALP were significantly lower suggesting that the O. stamineus ground leaf possibly do not cause kidney and liver impairment, mainly, at the higher dosage (8g/kg). Present study concluded that the broiler chicken fed O. stamineus ground leaf at a rate 8 g/kg was the most promising dietary supplement to enhance health without deleterious effects on serum biochemical properties and morphological components of liver. In addition, it reduces abdominal fats and serum cholesterol. This study has provide evident that medicinal plant, O. stamineus can potentially substituted the use of additive synthetic.
目的:评价饲粮中添加食材磨碎叶对大鼠血清生化指标和肝脏形态的影响。选取160只1日龄雄性肉仔鸡160只，随机分为4个处理组，每组5个重复，每组8只鸡:对照组(不添加饲料);组饲粮处理为:饲粮OS2(基础饲粮+ 2g/kg O. stamineus);饲粮OS4(基础饲粮+ 4g/kg O. staminees)和饲粮OS8(基础饲粮+ 8g/kg O. staminees)。42 d后，随机选取40只鸡进行血清生化分析，包括胰腺、肾脏和肝脏功能(尿素、钠、钾、氯、天冬氨酸转氨酶(AST)、碱性转氨酶(ALT)、碱性磷酸酶(ALP)、葡萄糖、胆固醇、甘油三酯、总蛋白、白蛋白和球蛋白)。本研究发现，血清胆固醇、甘油三酯、尿素、谷丙转氨酶、谷丙转氨酶和碱性磷酸酶水平均显著降低，提示在较高剂量(8g/kg)时，可能不会造成肾和肝损害。本研究认为，在不影响血清生化特性和肝脏形态成分的情况下，以8 g/kg的添加量饲喂玉米碎叶是最有希望提高肉鸡健康水平的饲粮。此外，它还能减少腹部脂肪和血清胆固醇。本研究证明了药用植物雄蕊草具有替代添加剂合成的潜力。
{"title":"Serum biochemical properties and liver morphology of broiler chicken as affected by feeding Misai kucing (Orthosiphon stamineus) as a supplementary diet","authors":"M. Malahubban, Zakry Fitri Ab Aziz","doi":"10.19071/RIB.2016.V7.3093","DOIUrl":"https://doi.org/10.19071/RIB.2016.V7.3093","url":null,"abstract":"To evaluate the effects of ground leaf of Misai kucing ( Orthosiphon stamineus ) as a dietary supplement on serum biochemical parameters and liver morphology. One hundred and sixty one-day old male broiler chickens (n=160) were distributed into four treatment groups, with five replicates of eight birds in each group: the control group (diet without additives); the group dietary treatments, Diet OS2 (Basal diet + 2g/kg O. stamineus ); Diet OS4 (Basal diet + 4g/kg O. stamineus ) and Diet OS8 (Basal diet + 8g/kg O. stamineus ). After 42 days, 40 birds were randomly selected for serum biochemical profile analysis involving pancreatic, renal, and hepatic functions (urea, sodium, potassium, chlorine, aspartate transaminase (AST), alkaline transaminase (ALT), alkaline phosphatase (ALP), glucose, cholesterol, triglycerides, total protein, albumin, and globulins). Present study found that serum levels of cholesterol, triglycerides, urea, AST, ALT and ALP were significantly lower suggesting that the O. stamineus ground leaf possibly do not cause kidney and liver impairment, mainly, at the higher dosage (8g/kg). Present study concluded that the broiler chicken fed O. stamineus ground leaf at a rate 8 g/kg was the most promising dietary supplement to enhance health without deleterious effects on serum biochemical properties and morphological components of liver. In addition, it reduces abdominal fats and serum cholesterol. This study has provide evident that medicinal plant, O. stamineus can potentially substituted the use of additive synthetic.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80568712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-10-28DOI: 10.19071/RIB.2016.V7.3078
E. Santos, Robson Antonio de Souza, M. J. Medeiros, G. D. Alves, L. Houllou
Two experiments were conducted to evaluate Bendazol fungicidal effects in neem micropropagation. In these experiments, the nodal segment explants from in vitro plants were used. In the first experiment, the explants remained in DKW culture medium for a period of 30 days containing different concentrations of Bendazol (M1 -50, M2 - 100, M3 - 200, and M4 - 400 mg.L -1 ). The control treatment (M0) was prepared with DKW medium + BAP (0.225 mg.L -1 ). In the second experiment, the explants were maintained for only one week in media supplemented with Bendazol or BAP, and then they were transferred and kept in free Bendazol/BAP media for three weeks. In each experiment, the design was completely randomized with five treatments, 10 replicates per treatment, and one explant/cultivation flask. The variables analyzed included the formation of calluses and roots, lateral bud development, shoot height, contamination and plant death. There was no significant difference in tree variables (shoot, callus formation and shoot height) between treatments in both experiments. There was no death, plant contamination and rooting during the experiment. The results indicate that Bendazol can be used at low doses for in vitro neem cloning thereby replacing BAP and ultimately reducing production costs.
{"title":"Effects of the bendazol fungicide on in vitro development of the nim (Azadirachta indica A. JUSS)","authors":"E. Santos, Robson Antonio de Souza, M. J. Medeiros, G. D. Alves, L. Houllou","doi":"10.19071/RIB.2016.V7.3078","DOIUrl":"https://doi.org/10.19071/RIB.2016.V7.3078","url":null,"abstract":"Two experiments were conducted to evaluate Bendazol fungicidal effects in neem micropropagation. In these experiments, the nodal segment explants from in vitro plants were used. In the first experiment, the explants remained in DKW culture medium for a period of 30 days containing different concentrations of Bendazol (M1 -50, M2 - 100, M3 - 200, and M4 - 400 mg.L -1 ). The control treatment (M0) was prepared with DKW medium + BAP (0.225 mg.L -1 ). In the second experiment, the explants were maintained for only one week in media supplemented with Bendazol or BAP, and then they were transferred and kept in free Bendazol/BAP media for three weeks. In each experiment, the design was completely randomized with five treatments, 10 replicates per treatment, and one explant/cultivation flask. The variables analyzed included the formation of calluses and roots, lateral bud development, shoot height, contamination and plant death. There was no significant difference in tree variables (shoot, callus formation and shoot height) between treatments in both experiments. There was no death, plant contamination and rooting during the experiment. The results indicate that Bendazol can be used at low doses for in vitro neem cloning thereby replacing BAP and ultimately reducing production costs.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73695518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-08-28DOI: 10.19071/rib.2016.v7.3037
A. Demissie, B. Chinthapalli, Shumet Tenaw, D. Chitra
Microalgae are considered as one of the potential source of biodiesel for the future. The search to obtain the potential strains from the algal diversity capable of producing oil is critical for sustainable production of biodiesel. In the present study, microalgae biomass with oil/lipid accumulation capability and their morphological features was isolated from Lake Abaya and Chamo. The algal biomass was cultivated in vitro and media optimization for maximum biomass was done using different basal media, BG-11 medium, and Chu -10. In addition the various carbon sources, nitrogen sources, pH and temperature were considered in this study for optimization. Green algae Oedogonium , Chlorella and Cladophora species were observed to be dominant species and the maximum oil per dry algal biomass was found to be from Oedogonium sp. Thus from the present study for the cultivation of the selected algae, BG-11 medium supplemented with tryptone (0.2%) sucrose (2%) and pH- 6 with incubation temperature of 30 0 C was found to be suitable. These results suggest that Oedogonium sp. has several desirable features that make it a potential candidate for biodiesel production.
{"title":"Cultivation of micro-algae for Production of Biodiesel: An optimized Process","authors":"A. Demissie, B. Chinthapalli, Shumet Tenaw, D. Chitra","doi":"10.19071/rib.2016.v7.3037","DOIUrl":"https://doi.org/10.19071/rib.2016.v7.3037","url":null,"abstract":"Microalgae are considered as one of the potential source of biodiesel for the future. The search to obtain the potential strains from the algal diversity capable of producing oil is critical for sustainable production of biodiesel. In the present study, microalgae biomass with oil/lipid accumulation capability and their morphological features was isolated from Lake Abaya and Chamo. The algal biomass was cultivated in vitro and media optimization for maximum biomass was done using different basal media, BG-11 medium, and Chu -10. In addition the various carbon sources, nitrogen sources, pH and temperature were considered in this study for optimization. Green algae Oedogonium , Chlorella and Cladophora species were observed to be dominant species and the maximum oil per dry algal biomass was found to be from Oedogonium sp. Thus from the present study for the cultivation of the selected algae, BG-11 medium supplemented with tryptone (0.2%) sucrose (2%) and pH- 6 with incubation temperature of 30 0 C was found to be suitable. These results suggest that Oedogonium sp. has several desirable features that make it a potential candidate for biodiesel production.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90757152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-08-08DOI: 10.19071/rib.2016.v7.3056
G. Torres, L. Houllou, Robson Antonio de Souza
The aim of this work was to test techniques to reduce microbial contamination in the phases of introduction and establishment of the in vitro cultivation of Bambusa vulgaris through two experiments. The first experiment was carried out in a completely randomized design using a factorial arrangement (pre-treatment of nodal segments using or not a solution of Derosal 500 SC ® and Chloramphenicol × culture medium with half or full concentration of salts × culture medium with presence or absence of sucrose × culture medium with presence or absence of Plant Preservative Mixture TM ). In a second experiment, carried out in a completely randomized design, the effect of different fungicides associated to Chloramphenicol in a liquid culture medium was tested. It was possible to verify that the isolated effects of the pre-treatment by immersion of the nodal segments in a solution of 4 mL L -1 of Derosal 500 SC ® and 200 mg L -1 of Chloramphenicol for 30 minutes and explants placed in a sucrose-free medium reduced fungal contamination. In the second experiment, the treatment that reduced fungal contamination corresponded to explants placed for seven days in a liquid medium with half the concentration of salts, sucrose-free, with 2 mL L -1 of Plant Preservative Mixture TM and with 4 mL L -1 of Derosal 500 SC ® and 200 mg L -1 of Chloramphenicol.
本研究通过两个实验，探讨在竹笋体外培养的引进和建立阶段减少微生物污染的技术。第一个实验采用完全随机设计，采用因子安排(预处理节段时使用或不使用Derosal 500 SC®和氯霉素×含一半或全部盐浓度的培养基×含或不含蔗糖的培养基×含或不含植物防腐剂混合物TM的培养基)。在第二个完全随机设计的实验中，测试了与氯霉素相关的不同杀菌剂在液体培养基中的效果。通过将节段浸泡在4 mL L -1的Derosal 500 SC®和200 mg L -1的氯霉素溶液中30分钟，并将外植体放置在无蔗糖培养基中，可以验证分离效果，减少真菌污染。在第二个实验中，减少真菌污染的处理对应于将外植体放置在含一半盐浓度的液体培养基中7天，无蔗糖，2 mL L -1 Plant Preservative Mixture TM, 4 mL L -1 Derosal 500 SC®和200 mg L -1氯霉素。
{"title":"Control of contaminants during introduction and establishment of Bambusa vulgaris in vitro","authors":"G. Torres, L. Houllou, Robson Antonio de Souza","doi":"10.19071/rib.2016.v7.3056","DOIUrl":"https://doi.org/10.19071/rib.2016.v7.3056","url":null,"abstract":"The aim of this work was to test techniques to reduce microbial contamination in the phases of introduction and establishment of the in vitro cultivation of Bambusa vulgaris through two experiments. The first experiment was carried out in a completely randomized design using a factorial arrangement (pre-treatment of nodal segments using or not a solution of Derosal 500 SC ® and Chloramphenicol × culture medium with half or full concentration of salts × culture medium with presence or absence of sucrose × culture medium with presence or absence of Plant Preservative Mixture TM ). In a second experiment, carried out in a completely randomized design, the effect of different fungicides associated to Chloramphenicol in a liquid culture medium was tested. It was possible to verify that the isolated effects of the pre-treatment by immersion of the nodal segments in a solution of 4 mL L -1 of Derosal 500 SC ® and 200 mg L -1 of Chloramphenicol for 30 minutes and explants placed in a sucrose-free medium reduced fungal contamination. In the second experiment, the treatment that reduced fungal contamination corresponded to explants placed for seven days in a liquid medium with half the concentration of salts, sucrose-free, with 2 mL L -1 of Plant Preservative Mixture TM and with 4 mL L -1 of Derosal 500 SC ® and 200 mg L -1 of Chloramphenicol.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84009385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-04-25DOI: 10.19071/RIB.2016.V7.2953
U. Bhavyashree, K. LakshmiJayaraj, T. P. Fayas, K. S. Muralikrishna, K. K. Sajini, M. K. Rajesh, A. Karun
A protocol was standardized to maximize yields of embryogenic calli from shoot meristem culture of coconut. Three different shoot meristem excision methods were tested viz ., excision of shoot meristem aseptically from in vitro germinated embryo after 10-12 days, excision of shoot meristem from in vitro germinated embryo subjected to GA3 treatment for five days and excision of shoot meristem from fresh embryo. The primary calli induction after 30 days of culture incubation for the three treatments were 21%, 27% and 79% respectively. Further, the primary calli formed from the shoot meristem excised from fresh embryo gave rise to 56% of embryogenic calli. The calli obtained from the shoot meristem which were excised from in vitro germinated embryo formed less percentage of embryogenic calli because of the presence of cotyledonary tissues which inhibited the multiplication of meristematic tissues. In the case of shoot meristem extracted from GA3-treated embryos, the percentage of non-embryogenic calli was more compared to the shoot meristem excised from fresh embryo. It was observed that the addition of GA3 in the initial stages of culture inhibited the formation of embryogenic calli and favored direct shoot development. Currently, the shoot meristem excised from fresh embryo is being employed for scaling up the planting material production from released varieties of coconut.
{"title":"A comparative study of three different methods of shoot meristem excision for induction of embryogenic calli in coconut","authors":"U. Bhavyashree, K. LakshmiJayaraj, T. P. Fayas, K. S. Muralikrishna, K. K. Sajini, M. K. Rajesh, A. Karun","doi":"10.19071/RIB.2016.V7.2953","DOIUrl":"https://doi.org/10.19071/RIB.2016.V7.2953","url":null,"abstract":"A protocol was standardized to maximize yields of embryogenic calli from shoot meristem culture of coconut. Three different shoot meristem excision methods were tested viz ., excision of shoot meristem aseptically from in vitro germinated embryo after 10-12 days, excision of shoot meristem from in vitro germinated embryo subjected to GA3 treatment for five days and excision of shoot meristem from fresh embryo. The primary calli induction after 30 days of culture incubation for the three treatments were 21%, 27% and 79% respectively. Further, the primary calli formed from the shoot meristem excised from fresh embryo gave rise to 56% of embryogenic calli. The calli obtained from the shoot meristem which were excised from in vitro germinated embryo formed less percentage of embryogenic calli because of the presence of cotyledonary tissues which inhibited the multiplication of meristematic tissues. In the case of shoot meristem extracted from GA3-treated embryos, the percentage of non-embryogenic calli was more compared to the shoot meristem excised from fresh embryo. It was observed that the addition of GA3 in the initial stages of culture inhibited the formation of embryogenic calli and favored direct shoot development. Currently, the shoot meristem excised from fresh embryo is being employed for scaling up the planting material production from released varieties of coconut.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87961050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-23DOI: 10.19071/RIB.2016.V7.2895
S. Tripathy, P. Mohanty, M. Jena, S. Dash, D. Lenka, D. Mishra, P. Nayak, D. Swain, R. Ranjan, K. Pradhan, N. Senapati, P. Mohapatra, G. Dash
A set of 292 mungbean germplasm accessions including 62 popularly adapted local land races and two wild forms ( Vigna radiata var. sublobata), important breeding lines and standard ruling varieties were screened for drought stress tolerance at seedling stage. Eight genotypes e.g., C. No. 35, OUM 14-1, OUM 49-2, Pusa 9072, OM 99-3, Banapur local B, Nipania munga, Kalamunga 1-A) have been identified to possess drought tolerance. Globulin seed storage protein profiling was carried out in 19 selected mungbean genotypes comprising eight drought tolerant, seven drought sensitive, two wild forms of mungbean (TCR 20 and TCR 213) and two standard checks (LGG 460 and T 2-1 ) to explore differentially expressed polypeptides. Seed protein profiles revealed 15 scorable polypeptide bands with molecular weights ranging from 10.0 to 102.2kD. A specific 12.8kD polypeptide band was present in all above drought tolerant test genotypes including the wild accession TCR 20. Such a polypeptide band may serve as useful biochemical marker for identification of drought tolerant genotypes in mungbean. Key words: Genetic diversity, seed storage protein profile, wild and cultivated Vigna radiata.
对292份绿豆种质资源进行了苗期抗旱性筛选，其中包括62个地方广适陆地小种和2个野生品种(Vigna radiata var. sublobata)、重要育种系和标准优势品种。8个基因型(C. 35、OUM 14-1、OUM 49-2、Pusa 9072、om99 -3、Banapur local B、Nipania munga、Kalamunga 1-A)均具有耐旱性。对19个绿豆基因型(8个抗旱型、7个抗旱型)、2个野生型(TCR 20和TCR 213)和2个标准型(LGG 460和t1 -1)进行了球蛋白种子贮藏蛋白分析，以探究其差异表达多肽。种子蛋白谱显示15个可评分的多肽带，分子量在10.0 ~ 102.2kD之间。包括野生植株TCR 20在内的所有抗旱试验基因型均存在一个特异的12.8kD多肽带。该多肽带可作为绿豆耐旱基因型鉴定的有用生化标记。关键词:遗传多样性，种子贮藏蛋白谱，野生和栽培野豇豆
{"title":"Identification of seed storage protein markers for drought tolerance in mungbean","authors":"S. Tripathy, P. Mohanty, M. Jena, S. Dash, D. Lenka, D. Mishra, P. Nayak, D. Swain, R. Ranjan, K. Pradhan, N. Senapati, P. Mohapatra, G. Dash","doi":"10.19071/RIB.2016.V7.2895","DOIUrl":"https://doi.org/10.19071/RIB.2016.V7.2895","url":null,"abstract":"A set of 292 mungbean germplasm accessions including 62 popularly adapted local land races and two wild forms ( Vigna radiata var. sublobata), important breeding lines and standard ruling varieties were screened for drought stress tolerance at seedling stage. Eight genotypes e.g., C. No. 35, OUM 14-1, OUM 49-2, Pusa 9072, OM 99-3, Banapur local B, Nipania munga, Kalamunga 1-A) have been identified to possess drought tolerance. Globulin seed storage protein profiling was carried out in 19 selected mungbean genotypes comprising eight drought tolerant, seven drought sensitive, two wild forms of mungbean (TCR 20 and TCR 213) and two standard checks (LGG 460 and T 2-1 ) to explore differentially expressed polypeptides. Seed protein profiles revealed 15 scorable polypeptide bands with molecular weights ranging from 10.0 to 102.2kD. A specific 12.8kD polypeptide band was present in all above drought tolerant test genotypes including the wild accession TCR 20. Such a polypeptide band may serve as useful biochemical marker for identification of drought tolerant genotypes in mungbean. Key words: Genetic diversity, seed storage protein profile, wild and cultivated Vigna radiata.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"637 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77027035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-02-10DOI: 10.19071/rib.2016.v7.2930
D. Chitra, B. Chinthapalli, G. Padmaja
Micropropagated and stem cutting derived plants of Mulberry ( Morus indica L. cv. S-36) were transferred to the similar field conditions. A comparative study was conducted based on morphological parameters and growth characteristics for three consecutive years. The results demonstrated that micropropagation gave rise to superior clonal populations with respect to number of branches/plant and leaf yield/plant that will be suitable for the mass production of plants. Thus in vitro grown plants did not exhibit any significant quantitative variation as compared to the conventionally grown plants, indicating the varietal multiplication to be of true-to-type.
桑树(Morus indica L. cv.)的微繁及茎切源植物。S-36)被转移到类似的野外条件。根据形态参数和生长特性连续3年进行对比研究。结果表明，微繁繁殖产生的无性系群体在枝/株数和叶/株产量方面均具有优势，适合大规模生产。因此，与常规栽培植株相比，离体栽培植株没有表现出任何显著的数量变异，表明品种繁殖是真实的。
{"title":"A comparative study on field performance of micropropagated plants and stem cutting derived plants of S-36 cultivar of Mulberry (Morus indica L.)","authors":"D. Chitra, B. Chinthapalli, G. Padmaja","doi":"10.19071/rib.2016.v7.2930","DOIUrl":"https://doi.org/10.19071/rib.2016.v7.2930","url":null,"abstract":"Micropropagated and stem cutting derived plants of Mulberry ( Morus indica L. cv. S-36) were transferred to the similar field conditions. A comparative study was conducted based on morphological parameters and growth characteristics for three consecutive years. The results demonstrated that micropropagation gave rise to superior clonal populations with respect to number of branches/plant and leaf yield/plant that will be suitable for the mass production of plants. Thus in vitro grown plants did not exhibit any significant quantitative variation as compared to the conventionally grown plants, indicating the varietal multiplication to be of true-to-type.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"113 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78528841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-02-10DOI: 10.19071/RIB.2016.V7.2981
C. L. Djimeli, A. T. Arfao, V. Rossi, N. Nsulem, V. Raspal, G. Bricheux, M. Nola, T. Sime-Ngando
After cell adhesion processes in microcosm, the impact of sodium hypochlorite (NaOCl) and hydrogen peroxide (H 2 O 2 ) on the detachment of Enterococcus faecalis from polythene fragments immersed in water under stationary and dynamic conditions was assessed. The abundance of planktonic cells was also evaluated. The density of E. faecalis adhered in absence of disinfectant fluctuated between 2 and 4 units (Log CFU/cm 2 ). After living in disinfected water, the density of E. faecalis remained adhered to polythene sometimes reached 2 units (Log CFU/Cm 2 ) . This highest abundance of cells remained adhered was recorded with cells coming from the lag, exponential and stationary growth phases in water treated with 0.5‰ NaOCl. In H 2 O 2 disinfected water, the highest value was recorded at all cells growth phases with 5‰ H 2 O 2 concentration. Adhered E. faecalis cells have been sometimes completely or partially decimated respectively by NaOCl and H 2 O 2 treated water. Considering separately each experimental condition, it was noted that increasing the concentration of disinfectant caused a significant decrease (P≤0.01) in abundance of cells stay adhered after living in water disinfected by the two disinfectants. Changes in disinfectant concentrations in different experimental conditions had an impact on the detachment of E. faecalis cells from the substrates.
{"title":"Impact of two disinfectants on detachment of Enterococcus faecalis from polythene in aquatic microcosm","authors":"C. L. Djimeli, A. T. Arfao, V. Rossi, N. Nsulem, V. Raspal, G. Bricheux, M. Nola, T. Sime-Ngando","doi":"10.19071/RIB.2016.V7.2981","DOIUrl":"https://doi.org/10.19071/RIB.2016.V7.2981","url":null,"abstract":"After cell adhesion processes in microcosm, the impact of sodium hypochlorite (NaOCl) and hydrogen peroxide (H 2 O 2 ) on the detachment of Enterococcus faecalis from polythene fragments immersed in water under stationary and dynamic conditions was assessed. The abundance of planktonic cells was also evaluated. The density of E. faecalis adhered in absence of disinfectant fluctuated between 2 and 4 units (Log CFU/cm 2 ). After living in disinfected water, the density of E. faecalis remained adhered to polythene sometimes reached 2 units (Log CFU/Cm 2 ) . This highest abundance of cells remained adhered was recorded with cells coming from the lag, exponential and stationary growth phases in water treated with 0.5‰ NaOCl. In H 2 O 2 disinfected water, the highest value was recorded at all cells growth phases with 5‰ H 2 O 2 concentration. Adhered E. faecalis cells have been sometimes completely or partially decimated respectively by NaOCl and H 2 O 2 treated water. Considering separately each experimental condition, it was noted that increasing the concentration of disinfectant caused a significant decrease (P≤0.01) in abundance of cells stay adhered after living in water disinfected by the two disinfectants. Changes in disinfectant concentrations in different experimental conditions had an impact on the detachment of E. faecalis cells from the substrates.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72693008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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