Pub Date : 2023-10-17DOI: 10.25081/rib.2023.v14.8618
Divya Bharathi Hinduja, A. S. Dhanu, G. G. Swamy, Karthikeyan Murugesan, Boojhana Elango, Uthamalingam Murali, Mamunur Rashid, Maghimaa Mathanmohun, Kanthesh M. Basalingappa
Maintaining a healthy diet is crucial for preventing cancer, as it provides the essential nutrients needed for proper physiological functioning. It is predicted that simple lifestyle and dietary changes can lessen the risk of developing 30-40% of all malignancies. Obesity, the consumption of nutrient-deficient foods such as sugary and refined flour products, which can lead to impaired glucose metabolism and, eventually, diabetes, a lack of dietary fiber, an excess of red meat, and an imbalance in the consumption of omega-3 and omega-6 fats are all risk factors for cancer. To reduce your risk of cancer, include flax seeds, a variety of fruits and vegetables, and dietary fiber in your diet. Additionally, there is proof that nutritional supplements may help lower the risk of breast cancer recurrence. To prevent various types of cancer, it is important to include vegetables, fruits, whole grains, and specific fatty acids in your diet, alongside engaging in regular physical exercise. Furthermore, it is crucial to use advances in genetics and molecular biology to extend nutritional research from observational studies to demonstrating causative linkages. Cancer prevention strategies that involve dietary changes targeted at specific groups should be based on a thorough understanding of these fundamental principles. Such dietary methods can be effective as well as in cancer prevention but also cancer rehabilitation. This review investigates the relationship between cancer and diet, examines straightforward approaches to incorporating cancer-preventive foods into one’s diet, investigates the impact of dietary variables and lifestyle choices on the risk of cancer, and investigates clinical studies focused on nutrition and cancer prevention.
{"title":"Diet and nutrition strategies for cancer prevention: A comprehensive review","authors":"Divya Bharathi Hinduja, A. S. Dhanu, G. G. Swamy, Karthikeyan Murugesan, Boojhana Elango, Uthamalingam Murali, Mamunur Rashid, Maghimaa Mathanmohun, Kanthesh M. Basalingappa","doi":"10.25081/rib.2023.v14.8618","DOIUrl":"https://doi.org/10.25081/rib.2023.v14.8618","url":null,"abstract":"Maintaining a healthy diet is crucial for preventing cancer, as it provides the essential nutrients needed for proper physiological functioning. It is predicted that simple lifestyle and dietary changes can lessen the risk of developing 30-40% of all malignancies. Obesity, the consumption of nutrient-deficient foods such as sugary and refined flour products, which can lead to impaired glucose metabolism and, eventually, diabetes, a lack of dietary fiber, an excess of red meat, and an imbalance in the consumption of omega-3 and omega-6 fats are all risk factors for cancer. To reduce your risk of cancer, include flax seeds, a variety of fruits and vegetables, and dietary fiber in your diet. Additionally, there is proof that nutritional supplements may help lower the risk of breast cancer recurrence. To prevent various types of cancer, it is important to include vegetables, fruits, whole grains, and specific fatty acids in your diet, alongside engaging in regular physical exercise. Furthermore, it is crucial to use advances in genetics and molecular biology to extend nutritional research from observational studies to demonstrating causative linkages. Cancer prevention strategies that involve dietary changes targeted at specific groups should be based on a thorough understanding of these fundamental principles. Such dietary methods can be effective as well as in cancer prevention but also cancer rehabilitation. This review investigates the relationship between cancer and diet, examines straightforward approaches to incorporating cancer-preventive foods into one’s diet, investigates the impact of dietary variables and lifestyle choices on the risk of cancer, and investigates clinical studies focused on nutrition and cancer prevention.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136034527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-13DOI: 10.25081/rib.2023.v14.8214
Enow Albert, N. Bille, N. Leonard
Digitalization in agriculture requires critical research into applications of artificial intelligence to various specialization domains. This work aimed at investigating the application of image synthesis technology to the mitigation of the data volume constraint to digital plant disease phenotyping accuracy. We designed an experiment involving the use of a deep convolutional generative adversarial network (DC-GAN) to synthesize photorealistic data for healthy and bacterial spot disease-infected tomato leaves. The training dataset contained 1,272 instances per class. We further employed a 3-block visual geometry group (VGG) convolutional neural network (CNN) model with dropout regularization and 1 epoch to compare classification accuracies of the original dataset and various synthetic datasets. Our results showed that the third DC-GAN synthesized training dataset containing 3,816 synthetic examples of both healthy and bacterial spot infected tomato leaf classes outperformed the original training dataset containing 1,272 real examples of both tomato leaf classes (77.088% accuracy with the former dataset on a 3-block VGG CNN model with dropout regularization and 1 epoch, as compared to 76.447% accuracy with the latter dataset on the same classifier).
{"title":"Improvement of plant disease classification accuracy with generative model-synthesized training datasets","authors":"Enow Albert, N. Bille, N. Leonard","doi":"10.25081/rib.2023.v14.8214","DOIUrl":"https://doi.org/10.25081/rib.2023.v14.8214","url":null,"abstract":"Digitalization in agriculture requires critical research into applications of artificial intelligence to various specialization domains. This work aimed at investigating the application of image synthesis technology to the mitigation of the data volume constraint to digital plant disease phenotyping accuracy. We designed an experiment involving the use of a deep convolutional generative adversarial network (DC-GAN) to synthesize photorealistic data for healthy and bacterial spot disease-infected tomato leaves. The training dataset contained 1,272 instances per class. We further employed a 3-block visual geometry group (VGG) convolutional neural network (CNN) model with dropout regularization and 1 epoch to compare classification accuracies of the original dataset and various synthetic datasets. Our results showed that the third DC-GAN synthesized training dataset containing 3,816 synthetic examples of both healthy and bacterial spot infected tomato leaf classes outperformed the original training dataset containing 1,272 real examples of both tomato leaf classes (77.088% accuracy with the former dataset on a 3-block VGG CNN model with dropout regularization and 1 epoch, as compared to 76.447% accuracy with the latter dataset on the same classifier).","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73170446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-06DOI: 10.25081/rib.2022.v13.7490
J. Obara, R. Mulwa, M. Oyoo, Miriam Karwitha
Maize production and productivity is on a sharp decline due to abiotic and abiotic stresses, therefore, an efficient regeneration protocol is an important tool that can contribute to maize improvement and gene-function studies to improve food security for the ever-growing population. The objective of this study was to optimize a regeneration system for CML 444 inbred line with CML 442 maize inbred line used as a reference. Callus was generated by incubation of immature embryos in Murashige and Skoog (MS) medium with vitamins supplemented with 0 - 4 g L-1 of 2, 4-D hormones, 900 mg L-1 proline, 250 mg L-1casein hydrolysate and 10 mg L-1 of filter sterilized AgNO3, 30 g L-l of sucrose and 3 g L-1 gelrite. Somatic embryo maturation was achieved by transferring 6-week old callus to MS medium with vitamins prepared as previously in callus induction with 60 g L-1 of sucrose and zero plant growth regulators (PGR). Shoot initiation was conducted in MS medium with vitamins supplemented with BAP, NAA at varied concentrations and a 0 mg L-1 control. Plants at a 3-leaf stage that had not rooted were transferred to MS media with vitamins with IBA at a concentration of 0 - 0.3 mg L–l. The 2, 4-D rates were significantly (p≤0.001) different for callus onset and callus induction. The genotype × rate interaction effects showed that 0 and 2 g L-1 2, 4-D had the lowest and highest mean, respectively in both lines during onset and induction of callus. The lines had significant (p≤0.001) effects on shooting induction, however, their means were not significantly different. Similarly, the means for the hormones were not significantly different for shooting induction. The lines, IBA rate and their interaction were significantly (p≤0.05) different for rooting induction. The means for the lines were significantly different for rooting induction in different IBA rates. Conversely, the mean for the IBA rates was significantly different for rooting induction. This study found that plant growth regulators rates during the callus induction stage play a key role during regeneration. This protocol was a success and could provide a fundamental platform for future transformation in this line.
由于非生物和非生物胁迫，玉米产量和生产力急剧下降，因此，有效的再生方案是促进玉米改良和基因功能研究的重要工具，可以为不断增长的人口改善粮食安全。以玉米CML 442自交系为参照，对CML 444自交系的再生体系进行了优化。未成熟胚在Murashige和Skoog (MS)培养基中培养愈伤组织，培养基中添加0 ~ 4 g L-1 2,4 - d激素、900 mg L-1脯氨酸、250 mg L-1酪蛋白水解物和10 mg L-1过滤灭菌AgNO3、30 g L-1蔗糖和3 g L-1明胶。将6周大的愈伤组织转移到含有维生素的MS培养基上，培养基中添加60 g L-1的蔗糖和零植物生长调节剂(PGR)，使愈伤组织成熟。在添加不同浓度BAP、NAA和0 mg L-1对照的维生素MS培养基中进行芽化。将未生根的3叶期植株转移到含有维生素和IBA的MS培养基上，浓度为0 - 0.3 mg L-l。愈伤组织发生和愈伤组织诱导的2,4 - d率差异显著(p≤0.001)。基因型×率互作效应表明，0和2 g L-1 2,4 - d分别在愈伤组织发生和诱导过程中具有最低和最高的平均值。各品系在诱导上有显著(p≤0.001)的效应，但其均值差异不显著。同样，激素对射击诱导的平均值也没有显著差异。在诱导生根方面，品系、IBA率及其互作量差异显著(p≤0.05)。不同IBA浓度下，不同品系的生根诱导能力有显著差异。相反，生根诱导的IBA平均速率有显著差异。本研究发现，愈伤组织诱导阶段植物生长调节剂的含量在愈伤组织再生过程中起关键作用。该协议是成功的，可以为这方面的未来转型提供一个基本平台。
{"title":"Somatic embryogenesis and optimization of regeneration system from immature embryos in maize inbred lines","authors":"J. Obara, R. Mulwa, M. Oyoo, Miriam Karwitha","doi":"10.25081/rib.2022.v13.7490","DOIUrl":"https://doi.org/10.25081/rib.2022.v13.7490","url":null,"abstract":"Maize production and productivity is on a sharp decline due to abiotic and abiotic stresses, therefore, an efficient regeneration protocol is an important tool that can contribute to maize improvement and gene-function studies to improve food security for the ever-growing population. The objective of this study was to optimize a regeneration system for CML 444 inbred line with CML 442 maize inbred line used as a reference. Callus was generated by incubation of immature embryos in Murashige and Skoog (MS) medium with vitamins supplemented with 0 - 4 g L-1 of 2, 4-D hormones, 900 mg L-1 proline, 250 mg L-1casein hydrolysate and 10 mg L-1 of filter sterilized AgNO3, 30 g L-l of sucrose and 3 g L-1 gelrite. Somatic embryo maturation was achieved by transferring 6-week old callus to MS medium with vitamins prepared as previously in callus induction with 60 g L-1 of sucrose and zero plant growth regulators (PGR). Shoot initiation was conducted in MS medium with vitamins supplemented with BAP, NAA at varied concentrations and a 0 mg L-1 control. Plants at a 3-leaf stage that had not rooted were transferred to MS media with vitamins with IBA at a concentration of 0 - 0.3 mg L–l. The 2, 4-D rates were significantly (p≤0.001) different for callus onset and callus induction. The genotype × rate interaction effects showed that 0 and 2 g L-1 2, 4-D had the lowest and highest mean, respectively in both lines during onset and induction of callus. The lines had significant (p≤0.001) effects on shooting induction, however, their means were not significantly different. Similarly, the means for the hormones were not significantly different for shooting induction. The lines, IBA rate and their interaction were significantly (p≤0.05) different for rooting induction. The means for the lines were significantly different for rooting induction in different IBA rates. Conversely, the mean for the IBA rates was significantly different for rooting induction. This study found that plant growth regulators rates during the callus induction stage play a key role during regeneration. This protocol was a success and could provide a fundamental platform for future transformation in this line.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85136944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-02DOI: 10.25081/rib.2021.v12.6859
J. Obara, W. Abincha
The ubiquitin/26S proteasome pathway is key to protein degradation in plants. Its specificity often orchestrated by ubiquitin-protein ligases (or E3s), which facilitate the translocation of ubiquitin to appropriate targets. F-box protein is one of the subunit of E3 ligases SCF (Skp1-Cullin/CDC53-F-box). It has been reported that F-box protein is not only related to plant growth but also abiotic stress. In this study, the protein was found localised in the nucleus and its function was identified. It demonstrated that on salt treatment SDR is involved in salt and drought stress response in Arabidopsis. However, the function of most F-box proteins is unknown. In this paper, the full length of the F-box protein SDR gene was cloned by traditional reverse molecular biology methods, and related transgenic materials were constructed. Bioinformatics analysis of the cis-element of the promoter of F-box protein was used to screen F-box proteins that may be stressed by plants. We found a large number of abiotic stress response elements such as drought stress response elements, salt stress response elements, and heat shock response elements in the promoter sequence upstream of the SDR (At5g15710) gene. The results show that SDR can be induced by ABA, heat shock, and salt, but expression is suppressed under drought treatment.
{"title":"The regulation of salt and drought stress responses by SDR and its interacting proteins in arabidopsis","authors":"J. Obara, W. Abincha","doi":"10.25081/rib.2021.v12.6859","DOIUrl":"https://doi.org/10.25081/rib.2021.v12.6859","url":null,"abstract":"The ubiquitin/26S proteasome pathway is key to protein degradation in plants. Its specificity often orchestrated by ubiquitin-protein ligases (or E3s), which facilitate the translocation of ubiquitin to appropriate targets. F-box protein is one of the subunit of E3 ligases SCF (Skp1-Cullin/CDC53-F-box). It has been reported that F-box protein is not only related to plant growth but also abiotic stress. In this study, the protein was found localised in the nucleus and its function was identified. It demonstrated that on salt treatment SDR is involved in salt and drought stress response in Arabidopsis. However, the function of most F-box proteins is unknown. In this paper, the full length of the F-box protein SDR gene was cloned by traditional reverse molecular biology methods, and related transgenic materials were constructed. Bioinformatics analysis of the cis-element of the promoter of F-box protein was used to screen F-box proteins that may be stressed by plants. We found a large number of abiotic stress response elements such as drought stress response elements, salt stress response elements, and heat shock response elements in the promoter sequence upstream of the SDR (At5g15710) gene. The results show that SDR can be induced by ABA, heat shock, and salt, but expression is suppressed under drought treatment.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"492 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76387649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.25081/RIB.2019.V10.5513
Ratan Chandro Mohanto, S. Shahriar, A. Ahmmed, Farzana Afrin Nishi, Most. Nurjahan Khatun Eaty
The experiment was carried out to evaluate the prevalence of seed-borne fungi of ten selected vegetables seeds e.g. amaranth, Indian spinach, bottle gourd, sweet gourd, snake gourd, okra, bitter gourd, cucumber, brinjal and country bean. Untreated and unknown grower’s bulk vegetables loose seeds were collected from three different seed sources of Rangpur district, Bangladesh e.g. New Bangla Seeds; Islam Seeds and Rafiq Traders. The high level of infection was found in the seeds of Rafiq Traders and the lowest infection was observed in the seeds of New Bangla Seeds. Islam Seeds provided moderate infection of fungal pathogens. Fungal fruiting bodies, mycelium (white and cottony) and acervuli were found under microscopic observation. The observed physical abnormalities viz. deformed, shrinkage, swelling, spotted and undersized seeds were recorded from all seed sources. Aspergillus flavus, Aspergillus niger, Fusarium sp., Alternaria sp., Chaetomium sp., Rhizopus sp. and unidentified bacteria were observed by blotter method. Aspergillus flavus showed maximum incidence (9.0%) in okra and minimum incidence (5.50%) in brinjal. Aspergillus niger was the most predominant (9.0%) in sweet gourd and minor (5.0%) in bottle gourd. Incidence of Fusarium sp. (5.0%) and Rhizopus sp. (8.75%) were predominant in bottle gourd. The lowest incidence for Fusarium sp. (2.50%) and Rhizopus sp. (3.0%) were found in snake gourd and amaranth respectively. The highest incidence of Alternaria sp. (4.75%) and Chaetomium sp. (9.0%) were found both in sweet gourd and bitter gourd but minimum incidence of Alternaria sp. (2.0%) and Chaetomium (3.75%) were recorded in amaranth and okra respectively. In case of unidentified bacteria, maximum incidence (4.50%) was recorded in bottle gourd.
{"title":"Prevalence of seed-borne fungi of different vegetables seeds in Bangladesh","authors":"Ratan Chandro Mohanto, S. Shahriar, A. Ahmmed, Farzana Afrin Nishi, Most. Nurjahan Khatun Eaty","doi":"10.25081/RIB.2019.V10.5513","DOIUrl":"https://doi.org/10.25081/RIB.2019.V10.5513","url":null,"abstract":"The experiment was carried out to evaluate the prevalence of seed-borne fungi of ten selected vegetables seeds e.g. amaranth, Indian spinach, bottle gourd, sweet gourd, snake gourd, okra, bitter gourd, cucumber, brinjal and country bean. Untreated and unknown grower’s bulk vegetables loose seeds were collected from three different seed sources of Rangpur district, Bangladesh e.g. New Bangla Seeds; Islam Seeds and Rafiq Traders. The high level of infection was found in the seeds of Rafiq Traders and the lowest infection was observed in the seeds of New Bangla Seeds. Islam Seeds provided moderate infection of fungal pathogens. Fungal fruiting bodies, mycelium (white and cottony) and acervuli were found under microscopic observation. The observed physical abnormalities viz. deformed, shrinkage, swelling, spotted and undersized seeds were recorded from all seed sources. Aspergillus flavus, Aspergillus niger, Fusarium sp., Alternaria sp., Chaetomium sp., Rhizopus sp. and unidentified bacteria were observed by blotter method. Aspergillus flavus showed maximum incidence (9.0%) in okra and minimum incidence (5.50%) in brinjal. Aspergillus niger was the most predominant (9.0%) in sweet gourd and minor (5.0%) in bottle gourd. Incidence of Fusarium sp. (5.0%) and Rhizopus sp. (8.75%) were predominant in bottle gourd. The lowest incidence for Fusarium sp. (2.50%) and Rhizopus sp. (3.0%) were found in snake gourd and amaranth respectively. The highest incidence of Alternaria sp. (4.75%) and Chaetomium sp. (9.0%) were found both in sweet gourd and bitter gourd but minimum incidence of Alternaria sp. (2.0%) and Chaetomium (3.75%) were recorded in amaranth and okra respectively. In case of unidentified bacteria, maximum incidence (4.50%) was recorded in bottle gourd.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"310 10 1","pages":"01-05"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86610961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-13DOI: 10.25081/RIB.2018.V9.3686
Nor Hafishah Md Zohir, Zakry Fitri Ab Aziz, A. Rajaee, M. Malahubban
Present study demonstrated the antibacterial potential of three different body parts, abdomen, carapace and cheliped of mud lobster (Thalassina anomala) which were extracted with methanol and hexane. Three test bacteria were used in this study namely Bacillus cereus, Escherichia coli, and Salmonella enterica. Present study found that in general, methanolic and hexane extracts of different body parts had variable inhibitory responses on different test bacteria with methanolic extract had greater inhibitory response than hexane extract. In methanol extraction, B. cereus significantly had greatest negative impact (p<0.05) on growth after treated with cheliped (21 mm zone of inhibition) extract of mud lobster as compared to carapace (9.2 mm) and abdominal (8.8 mm) extracts of mud lobster. Overall, the growth of S. enterica was much affected with the presence of all methanolic body-part extracts of mud lobster. In hexane extraction, B. cereus growth was not affected with all body-part extracts of mud lobster. However, E. coli and S. enterica were affected with no significantly different (p>0.05) among body-part extracts and between them. Present study concludes that the extracts of mud lobster of all body-part in methanol possess greater inhibitory effect on bacterial growth than extracts in hexane thus the methanolic extract of mud lobster has the potential to be exploited as a natural source of antibacterial agent.
{"title":"Antibacterial potential of methanolic and hexanic extracts of mud lobster (Thalassina anomala) from Bintulu, Sarawak, Malaysia","authors":"Nor Hafishah Md Zohir, Zakry Fitri Ab Aziz, A. Rajaee, M. Malahubban","doi":"10.25081/RIB.2018.V9.3686","DOIUrl":"https://doi.org/10.25081/RIB.2018.V9.3686","url":null,"abstract":"Present study demonstrated the antibacterial potential of three different body parts, abdomen, carapace and cheliped of mud lobster (Thalassina anomala) which were extracted with methanol and hexane. Three test bacteria were used in this study namely Bacillus cereus, Escherichia coli, and Salmonella enterica. Present study found that in general, methanolic and hexane extracts of different body parts had variable inhibitory responses on different test bacteria with methanolic extract had greater inhibitory response than hexane extract. In methanol extraction, B. cereus significantly had greatest negative impact (p<0.05) on growth after treated with cheliped (21 mm zone of inhibition) extract of mud lobster as compared to carapace (9.2 mm) and abdominal (8.8 mm) extracts of mud lobster. Overall, the growth of S. enterica was much affected with the presence of all methanolic body-part extracts of mud lobster. In hexane extraction, B. cereus growth was not affected with all body-part extracts of mud lobster. However, E. coli and S. enterica were affected with no significantly different (p>0.05) among body-part extracts and between them. Present study concludes that the extracts of mud lobster of all body-part in methanol possess greater inhibitory effect on bacterial growth than extracts in hexane thus the methanolic extract of mud lobster has the potential to be exploited as a natural source of antibacterial agent.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88531714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-04DOI: 10.19071/RIB.2017.V8.3244
P. Santosh, S. B. Arakera
Couroupita guianensis Aubl. Commonly known as cannon ball tree, belongs to family Lecythidaceae and widely used in Chinese medicine. C. guianensis is being threatened due to habitat destruction in its native range for human settlement and other development related activities. Native populations of C. guianensis were collected from different locations of the India states and characterized through. To study the genetic diversity of C. guianensis populations we performed RAPD – PCR with several primers. The results are revealed in this study.
{"title":"Genetic Diversity Analysis of Couroupita guianensis Aubl.based on RAPD Marker","authors":"P. Santosh, S. B. Arakera","doi":"10.19071/RIB.2017.V8.3244","DOIUrl":"https://doi.org/10.19071/RIB.2017.V8.3244","url":null,"abstract":"Couroupita guianensis Aubl. Commonly known as cannon ball tree, belongs to family Lecythidaceae and widely used in Chinese medicine. C. guianensis is being threatened due to habitat destruction in its native range for human settlement and other development related activities. Native populations of C. guianensis were collected from different locations of the India states and characterized through. To study the genetic diversity of C. guianensis populations we performed RAPD – PCR with several primers. The results are revealed in this study.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"158 1","pages":"12-15"},"PeriodicalIF":0.0,"publicationDate":"2017-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76494989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-31DOI: 10.25081/RIB.2017.V8.3596
S. V. Pawar, Munneshkumar, V. Jambhale
Molecular diversity in thirty wheat genotypes was done. For this the genomic DNA isolation was carried out and which were then subjected to PCR amplification using twenty SSR primers. Out of these twenty SSR primers, eighteen yielded amplifications and showed polymorphism. Total 93 loci were generated by amplification with 18 polymorphic primers, all of which 93 loci were polymorphic i.e. 100%. Among the SSR primers, BARC-170, WMC-44, produced maximum number of 2 loci. The size of amplification products ranged from 102 bp to 805 bp. All SSR primers showed 100 % polymorphism and all primers had more than 0.50 PIC value except one primer. Maximum PIC value 0.17 was observed in WMC-468. The maximum number of bands were observed in NIAW-2721 (28 bands), whereas minimum number of bands were present in NIAW-301 and NIAW-2539 (19 bands). The dice similarity coefficient values ranged from 0.50 to 0.95. Maximum similarity value of 0.95 was noticed between NIAW-2891 and NIAW-2837, while minimum similarity value of 0.50 was observed among NIAW-2595, NIAW-2874, NIAW-2995 and NIAW-2725. The consensus tree software revealed two major clusters.
{"title":"MOLECULAR CHARACTERIZATION OF WHEAT GENOTYPES (TRITICUM AESTIVUM L.)","authors":"S. V. Pawar, Munneshkumar, V. Jambhale","doi":"10.25081/RIB.2017.V8.3596","DOIUrl":"https://doi.org/10.25081/RIB.2017.V8.3596","url":null,"abstract":"Molecular diversity in thirty wheat genotypes was done. For this the genomic DNA isolation was carried out and which were then subjected to PCR amplification using twenty SSR primers. Out of these twenty SSR primers, eighteen yielded amplifications and showed polymorphism. Total 93 loci were generated by amplification with 18 polymorphic primers, all of which 93 loci were polymorphic i.e. 100%. Among the SSR primers, BARC-170, WMC-44, produced maximum number of 2 loci. The size of amplification products ranged from 102 bp to 805 bp. All SSR primers showed 100 % polymorphism and all primers had more than 0.50 PIC value except one primer. Maximum PIC value 0.17 was observed in WMC-468. The maximum number of bands were observed in NIAW-2721 (28 bands), whereas minimum number of bands were present in NIAW-301 and NIAW-2539 (19 bands). The dice similarity coefficient values ranged from 0.50 to 0.95. Maximum similarity value of 0.95 was noticed between NIAW-2891 and NIAW-2837, while minimum similarity value of 0.50 was observed among NIAW-2595, NIAW-2874, NIAW-2995 and NIAW-2725. The consensus tree software revealed two major clusters.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"13 1","pages":"29-36"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83345670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-31DOI: 10.25081/RIB.2017.V8.3575
V. Jambhale, S. V. Pawar
Direct regeneration from mature embryo axes was achieved without intervening of callus phase in four chickpea varieties on the Media MS and B5 supplemented with combination of BAP, NAA and Kinetin. Hundred percent regeneration capacity was exhibited by commercially grown Vijay and Vishal varieties. There was considerable variation in umber of multiple shoot production by different varieties. Profuse rooting was obtained on the medium containing 0.5 and 1.0 mg/l IBA. This protocol is optimized for complete plant regeneration of chickpea for genetic transformation.
{"title":"DE NOVO REGENERATION IN CHICKPEA GENOTYPES (CICER ARIETINUM L.)","authors":"V. Jambhale, S. V. Pawar","doi":"10.25081/RIB.2017.V8.3575","DOIUrl":"https://doi.org/10.25081/RIB.2017.V8.3575","url":null,"abstract":"Direct regeneration from mature embryo axes was achieved without intervening of callus phase in four chickpea varieties on the Media MS and B5 supplemented with combination of BAP, NAA and Kinetin. Hundred percent regeneration capacity was exhibited by commercially grown Vijay and Vishal varieties. There was considerable variation in umber of multiple shoot production by different varieties. Profuse rooting was obtained on the medium containing 0.5 and 1.0 mg/l IBA. This protocol is optimized for complete plant regeneration of chickpea for genetic transformation.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"82 1","pages":"16-18"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75315445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-31DOI: 10.25081/RIB.2017.V8.3598
H. Najat, Hassan Benaouda Najat Arsalane
Morocco is one of the major exporters of small citrus fruits, such as mandarin and Clementine. Seedlessness is a major criterion for this horticultural group. The present study focused on the selection of the best triploid mandarin hybrids (2n=3x=27) characterized by seedless fruits. A series of crosses between ‘Sidi Aissa’ clementine (female parent) and seven mandarin varieties (‘Lee’, ‘Wilking’, ‘Osceola’, ‘Carvalhal’, ‘Satsuma Frost’, ‘Satsuma Owari’ and ‘Chienka’) was performed by the National Institute for Agricultural Research. Forty triploid mandarins were obtained and planted since 2002 in an experimental field at El Menzeh. Varietal evaluation was focusing on fruit quality traits during seven years. Statistical analyzes showed that there is a significant difference for all studied characters and between hybrids. The number of seeds per fruit is the main criterion which differentiates between triploids mandarin’s hybrids and their diploid parent “clementine Sidi Aissa”. The best hybrids selected were: HT11, HT13, HT27, HT43, HT44, and HT49. The best crosses are C1 (‘Sidi Aissa’ × ‘Wilking’) andC2 (‘Sidi Aissa’ בOsceola’). These promising triploid hybrids of mandarin have been multiplied on several rootstock trials and are in the process of quantitative evaluation and multi-site testing.
{"title":"SELECTION OF NEW PROMISING SEEDLESS MANDARINS TRIPLOID HYBRIDS FROM CROSSES BETWEEN MONOEMBRYONIC DIPLOID FEMALE AND DIPLOID MALE PARENTS","authors":"H. Najat, Hassan Benaouda Najat Arsalane","doi":"10.25081/RIB.2017.V8.3598","DOIUrl":"https://doi.org/10.25081/RIB.2017.V8.3598","url":null,"abstract":"Morocco is one of the major exporters of small citrus fruits, such as mandarin and Clementine. Seedlessness is a major criterion for this horticultural group. The present study focused on the selection of the best triploid mandarin hybrids (2n=3x=27) characterized by seedless fruits. A series of crosses between ‘Sidi Aissa’ clementine (female parent) and seven mandarin varieties (‘Lee’, ‘Wilking’, ‘Osceola’, ‘Carvalhal’, ‘Satsuma Frost’, ‘Satsuma Owari’ and ‘Chienka’) was performed by the National Institute for Agricultural Research. Forty triploid mandarins were obtained and planted since 2002 in an experimental field at El Menzeh. Varietal evaluation was focusing on fruit quality traits during seven years. Statistical analyzes showed that there is a significant difference for all studied characters and between hybrids. The number of seeds per fruit is the main criterion which differentiates between triploids mandarin’s hybrids and their diploid parent “clementine Sidi Aissa”. The best hybrids selected were: HT11, HT13, HT27, HT43, HT44, and HT49. The best crosses are C1 (‘Sidi Aissa’ × ‘Wilking’) andC2 (‘Sidi Aissa’ בOsceola’). These promising triploid hybrids of mandarin have been multiplied on several rootstock trials and are in the process of quantitative evaluation and multi-site testing.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"40 1","pages":"37-44"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84648134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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