Pub Date : 2016-02-06DOI: 10.19071/RIB.2016.V7.2818
Shahana Khan, R. Shah
Withania somnifera is commonly known as Indian Ginseng or Ashwagandha which is listed highly medicinal plant in Ayurveda for its wide range of medicinal use. Genetic diversity among 16 genotypes of Withania somnifera collected from four different regions viz. Lucknow, Nimuch, Karnataka and Mumbai was studied using RAPD and ISSR techniques. The ISSR and RAPD together produced 89 bands across 16 genotypes of W. somnifera of which 39 were polymorphic showing 47.89% polymorphism. Over 99% of the RAPD and ISSR fragments were reproducible in the present experiment. There was 90% uniformity within the population. The neighbor joining tree also confirmed a clear grouping and differentiation based on populations of origin i.e. samples were grouped on the basis of their regions of origin. The dendrogram reveals that outgroup of W. somnifera Karnataka origin are evolutionarily related to W. somnifera Nimuch group varieties and W. somnifera Lucknow and Mumbai group varieties are evolutionarily closely related. Within population analysis of Lucknow and Nimuch location showed two sub groups of two lines each showing slight diversity. Plants from Karnataka and Mumbai region show less diversity within population.
{"title":"Assessment of genetic diversity among India Ginseng, Withania somnifera (L) Dunal using RAPD and ISSR markers","authors":"Shahana Khan, R. Shah","doi":"10.19071/RIB.2016.V7.2818","DOIUrl":"https://doi.org/10.19071/RIB.2016.V7.2818","url":null,"abstract":"Withania somnifera is commonly known as Indian Ginseng or Ashwagandha which is listed highly medicinal plant in Ayurveda for its wide range of medicinal use. Genetic diversity among 16 genotypes of Withania somnifera collected from four different regions viz. Lucknow, Nimuch, Karnataka and Mumbai was studied using RAPD and ISSR techniques. The ISSR and RAPD together produced 89 bands across 16 genotypes of W. somnifera of which 39 were polymorphic showing 47.89% polymorphism. Over 99% of the RAPD and ISSR fragments were reproducible in the present experiment. There was 90% uniformity within the population. The neighbor joining tree also confirmed a clear grouping and differentiation based on populations of origin i.e. samples were grouped on the basis of their regions of origin. The dendrogram reveals that outgroup of W. somnifera Karnataka origin are evolutionarily related to W. somnifera Nimuch group varieties and W. somnifera Lucknow and Mumbai group varieties are evolutionarily closely related. Within population analysis of Lucknow and Nimuch location showed two sub groups of two lines each showing slight diversity. Plants from Karnataka and Mumbai region show less diversity within population.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80205942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-02-06DOI: 10.19071/RIB.2016.V7.2863
S. Tripathy, P. Mohanty, M. Jena, G. Dash, K. Pradhan, P. Nayak, S. Dash, D. Lenka, D. Mishra, P. Mohapatra, D. Swain, N. Senapati
Total seed storage protein profiles of 20 urdbean genotypes including the popular variety T9 were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 14 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into three protein types. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 78.5% phenon level. TU 95-1 with TU 12-25-4 revealed lowest similarity index value (0.33) followed by TU 95-1 with PU 30 and KU 96-3(SI=0.35). Clustering pattern revealed distinctly divergent group formed by TPU 95-1 and TPU 4. These may serve as a valuable source genotype in recombination breeding. Key words: Seed storage protein profiling, SDS-PAGE, Genetic variation, urdbean.
{"title":"Revealing contrasting genetic variation and study of genetic diversity in urdbean (Vigna mungo (L.) Hepper) using SDS-PAGE of seed storage proteins","authors":"S. Tripathy, P. Mohanty, M. Jena, G. Dash, K. Pradhan, P. Nayak, S. Dash, D. Lenka, D. Mishra, P. Mohapatra, D. Swain, N. Senapati","doi":"10.19071/RIB.2016.V7.2863","DOIUrl":"https://doi.org/10.19071/RIB.2016.V7.2863","url":null,"abstract":"Total seed storage protein profiles of 20 urdbean genotypes including the popular variety T9 were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 14 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into three protein types. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 78.5% phenon level. TU 95-1 with TU 12-25-4 revealed lowest similarity index value (0.33) followed by TU 95-1 with PU 30 and KU 96-3(SI=0.35). Clustering pattern revealed distinctly divergent group formed by TPU 95-1 and TPU 4. These may serve as a valuable source genotype in recombination breeding. Key words: Seed storage protein profiling, SDS-PAGE, Genetic variation, urdbean.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86701452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-10DOI: 10.4172/2375-4397.1000126
S. Noel, M. Rajan
Ground water from Chinnalapatti,TamilNadu, India, was collected from the habitations at the vicinity of dyeing industries to study the impact of dyeing industry effluent on ground water quality. Physico-chemical parameters, namely EC, pH, Total Hardness, Ca, Mg, Na, Cl, TDS, Potassium and Sulphate were analyzed and compared with drinking water standard. pH, Ca and Mg were within the desirable and permissible limit of drinking, whereas sulphate, Sodium and Potassium were within the desirable limit only. Water Quality Index was calculated to assess the level of pollution and it is 61, indicating the pollution rate of ground water between slight and moderate. Correlation of various physico-chemical parameters with EC, TDS, and Total hardness was also calculated to establish the nature of relationship between them. EC is positively correlated with sodium, similarly TDS and Total hardness are positively correlated with calcium.
{"title":"Impact of Dyeing Industry Effluent on Ground water Quality by Water Quality Index and Correlation Analysis","authors":"S. Noel, M. Rajan","doi":"10.4172/2375-4397.1000126","DOIUrl":"https://doi.org/10.4172/2375-4397.1000126","url":null,"abstract":"Ground water from Chinnalapatti,TamilNadu, India, was collected from the habitations at the vicinity of dyeing industries to study the impact of dyeing industry effluent on ground water quality. Physico-chemical parameters, namely EC, pH, Total Hardness, Ca, Mg, Na, Cl, TDS, Potassium and Sulphate were analyzed and compared with drinking water standard. pH, Ca and Mg were within the desirable and permissible limit of drinking, whereas sulphate, Sodium and Potassium were within the desirable limit only. Water Quality Index was calculated to assess the level of pollution and it is 61, indicating the pollution rate of ground water between slight and moderate. Correlation of various physico-chemical parameters with EC, TDS, and Total hardness was also calculated to establish the nature of relationship between them. EC is positively correlated with sodium, similarly TDS and Total hardness are positively correlated with calcium.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78983961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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