Reproductive biomedicine online最新文献

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IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online

Pub Date : 2024-09-12 DOI: 10.1016/S1472-6483(24)00616-3

引用次数: 0

Inside Front Cover - Affiliations and First page of TOC 封面内页 - 隶属机构和目录首页

IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online

Pub Date : 2024-09-12 DOI: 10.1016/S1472-6483(24)00606-0

引用次数: 0

Would surgical retrieval of product of conception for the purpose of genetic analysis increase the risk of intrauterine adhesions? 为进行基因分析而手术取出受孕产物是否会增加宫内粘连的风险？

IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online

Pub Date : 2024-09-11 DOI: 10.1016/j.rbmo.2024.104445

Lionel Reyftmann

引用次数: 0

Association of Vitamin D receptor gene polymorphisms (FokI, ApaI, TaqI), metabolic features and susceptibility to polycystic ovary syndrome: a preliminary single center case-control study 维生素 D 受体基因多态性（FokI、ApaI、TaqI）、代谢特征与多囊卵巢综合征易感性的关系：一项初步的单中心病例对照研究

IF 4 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online

Pub Date : 2024-09-08 DOI: 10.1016/j.rbmo.2024.104447

Talida Vulcan, Iancu Mihaela, Lucia Maria Procopciuc, Tudor Sergiu Suciu, Gabriela Adriana Filip

Are the combined genotypes and haplotypes of VDR gene polymorphisms () associated with PCOS susceptibility and metabolic features of the disease? We performed a case-control study, including 46 women with PCOS and 48 controls. Genotypes of VDR genes were determined using the PCR-RFLP (restriction fragment length polymorphism) method. In all women, the waist circumference, parameters of lipid and glucose metabolism were evaluated. PCOS group presented higher waist circumference and visceral adiposity index (VAI) levels compared to controls. Total cholesterol, LDL-C and triglycerides had higher values in PCOS women. VDR- C/C (F/F) genotype had significantly higher odds of PCOS (adjusted OR = 6.27, 95% CI: 1.53-25.65). VDR- was associated with susceptibility to PCOS in the dominant model, the variant genotype (A/C-A/A) (A/a- a/a) had higher odds of PCOS than the wild genotype C/C (A/A) (adjusted OR = 3.15, 95% CI: 1.07-9.32). Haplotype analysis revealed that T-C- T (f-A-T) haplotype was statistically associated with lower odds of PCOS (adjusted OR = 0.10, 95% CI: 0.01-0.95). PCOS women with VDR-Fokl T/T (f/f) genotype had lower fasting glucose and higher VAI levels compared to C/T (Ff) or C/C (F/F) genotype. The present findings suggest the association between and polymorphisms and PCOS susceptibility. Moreover, T/T (f/f) genotype of VDR- could be a marker of decreased fasting glucose in PCOS. polymorphism did not reveal a relationship with PCOS susceptibility in our study population.

VDR 基因多态性（）的组合基因型和单倍型是否与多囊卵巢综合征的易感性和该病的代谢特征有关？我们进行了一项病例对照研究，其中包括 46 名多囊卵巢综合征妇女和 48 名对照组妇女。采用 PCR-RFLP（限制性片段长度多态性）方法确定了 VDR 基因的基因型。对所有妇女的腰围、血脂和糖代谢参数进行了评估。与对照组相比，多囊卵巢综合征组的腰围和内脏脂肪指数（VAI）水平较高。多囊卵巢综合征妇女的总胆固醇、低密度脂蛋白胆固醇和甘油三酯的数值较高。VDR- C/C（F/F）基因型患多囊卵巢综合症的几率明显更高（调整后 OR = 6.27，95% CI：1.53-25.65）。在显性模型中，VDR- 与多囊卵巢综合征的易感性相关，变异基因型（A/C-A/A）（A/a- a/a）比野生基因型 C/C (A/A)患多囊卵巢综合征的几率更高（调整 OR = 3.15，95% CI：1.07-9.32）。单倍型分析表明，T-C- T（f-A-T）单倍型与多囊卵巢综合征的较低几率有统计学关联（调整后 OR = 0.10，95% CI：0.01-0.95）。与 C/T (Ff) 或 C/C (F/F) 基因型相比，具有 VDR-Fokl T/T (f/f) 基因型的多囊卵巢综合症女性空腹血糖较低，VAI 水平较高。本研究结果表明，和多态性与多囊卵巢综合征易感性之间存在关联。此外，在我们的研究人群中，VDR-的T/T（f/f）基因型可能是多囊卵巢综合征患者空腹血糖降低的标志物。

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引用次数: 0

Ovarian tissue cryopreservation in breast cancer patients: glass half empty or glass half full? 乳腺癌患者的卵巢组织冷冻：半杯空还是半杯满？

IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online

Pub Date : 2024-09-06 DOI: 10.1016/j.rbmo.2024.104442

Claus Y. Andersen , Jacques Donnez , Erik Ernst , Debbie Gook , Antonio Pellicer , Michael Von Wolff , Nao Suzuki , Christophe Roux , Marie-Madeleine Dolmans

This is a commentary to a paper recently published in RBMOnline by Macklon and De Vos, in which they argue for a discontinuation of ovarian tissue freezing for fertility preservation in women with breast cancer. Instead, they suggest the use of oocyte vitrification following ovarian stimulation as the preferred method of fertility preservation. This commentary presents nine separate arguments that should be considered in the context of ovarian tissue freezing and fertility preservation in girls and women. Collectively, the authors support ovarian tissue freezing going forward and suggest continuing this procedure for fertility preservation in women with breast cancer. Ovarian tissue freezing represents several advantages for patients and provides them with more options following treatment compared with oocyte vitrification.

本文是对 Macklon 和 De Vos 最近发表在《RBMOnline》上的一篇论文的评论，他们在文中主张停止将卵巢组织冷冻用于乳腺癌妇女的生育力保存。相反，他们建议将卵巢刺激后的卵母细胞玻璃化作为生育力保存的首选方法。本评论提出了九个不同的论点，这些论点应在女孩和妇女卵巢组织冷冻和生育力保存的背景下加以考虑。总之，作者支持继续进行卵巢组织冷冻，并建议乳腺癌妇女继续采用这种方法保留生育力。与卵母细胞玻璃化相比，卵巢组织冷冻为患者带来了多项优势，并为她们在治疗后提供了更多选择。

引用次数: 0

Donor sperm: only for the rich and straight. 捐献精子：只适合有钱人和正直的人。

IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online

Pub Date : 2024-09-03 DOI: 10.1016/j.rbmo.2024.104434

Guido Pennings

Although single women and lesbian couples are given access to donor spermatozoa, all kinds of restrictions limit their actual use of donor insemination. These restrictions are frequently justified by the increase in safety (lowering the genetic risk) or well-being of donor-conceived persons. However, these restrictions directly result in stratified reproduction in which only the rich (those with the necessary financial means) and the straight (those who conform to dominant views of parenthood) are allowed to reproduce.

虽然单身妇女和女同性恋夫妇可以获得捐精者的精子，但她们在实际使用捐精授精时受到各种限制。这些限制往往以提高安全性（降低遗传风险）或提高捐精受孕者的福利为理由。然而，这些限制直接导致了生育分层，只有富人（拥有必要经济能力的人）和异性恋者（符合主流生育观的人）才能生育。

引用次数: 0

Polar body-based PGT-A: not dead yet? A step forward back to the roots of PGT-A. 基于极地车身的 PGT-A：尚未消亡？回到 PGT-A 的根源，向前迈出一步。

IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online

Pub Date : 2024-08-30 DOI: 10.1016/j.rbmo.2024.104430

Anna Oberle, Michael Feichtinger

Trophectoderm-based preimplantation genetic testing for aneuploidies (PGT-A) is used worldwide as a means of selecting embryos with high potential for achieving a live birth. However, trophectoderm analysis may be impaired through embryonic mosaicism, leading to genetically healthy embryos being falsely discarded, and thus even reducing cumulative live birth rates. Polar body biopsy, a technique applied since the early days of preimplantation testing, has been abandoned by most IVF centres. In comparison to trophectoderm analysis, however, polar body biopsy might even have certain advantages over trophectoderm PGT-A. This Countercurrent contribution discusses the newest clinical evidence, as well as ethical and cost-efficiency considerations, and argue that polar body analysis should be reconsidered.

基于滋养层的胚胎植入前非整倍体基因检测（PGT-A）已在全球范围内得到广泛应用，作为一种选择极有可能实现活产的胚胎的手段。然而，滋养层分析可能会因胚胎嵌合而受到影响，导致基因健康的胚胎被错误地丢弃，从而甚至降低累积活产率。极体活检是植入前检测早期就开始应用的一项技术，目前已被大多数试管婴儿中心放弃。不过，与滋养层分析相比，极体活检甚至可能比滋养层 PGT-A 更有优势。这篇逆流投稿讨论了最新的临床证据以及伦理和成本效益方面的考虑，并认为应重新考虑极体分析。

引用次数: 0

Assessment of bisphenol accumulation from disposable devices used sequentially in IVF routine procedures 评估试管婴儿常规程序中连续使用的一次性设备中双酚的累积情况

IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online

Pub Date : 2024-08-29 DOI: 10.1016/j.rbmo.2024.104431

Lucie Delaroche , Lucile Besnard , Nino Guy Cassuto , Sébastien Bristeau , Anne Togola

Research question

Are bisphenols released from disposable devices used in assisted reproductive technology (ART) procedures, and do they accumulate when several disposable devices are used sequentially under routine conditions?

Design

A comprehensive assessment of 19 individual disposable devices (31 assessments) and nine combinations of disposable devices replicating the main steps in an ART procedure was undertaken. The extraction of bisphenols followed routine-use conditions (temperature and duration). The concentrations of 10 bisphenols were determined using online solid-phase extraction/liquid chromatography/mass spectrometry methodology.

Results

Bisphenol S (BPS) was quantified consistently from 100-mm culture dishes (32 ± 20 pg) and from high security sperm straws (3 ± 1 pg). Also, BPS and bisphenol A (BPA) were quantified consistently from spermicide-free condoms (95 ± 78 and 83 ± 49 pg, respectively). No other bisphenols were detected in disposable devices when tested individually. When disposable devices were used in combination, both BPA and BPS were detected consistently in combinations of 13 disposable devices mimicking sperm collection in a condom and its preparation (46 ± 16 and 43 ± 32 pg, respectively). BPS was quantified consistently in combinations of 14 disposable devices mimicking sperm collection, its preparation and freezing (10 ± 4 pg), and in combinations of 17 disposable devices mimicking oocyte retrieval (37 ± 22 pg).

Conclusions

BPA and BPS are released in small quantities from some disposable devices used in routine conditions during ART procedures, but do not appear to accumulate when these disposable devices are used in combination.

研究问题辅助生殖技术（ART）程序中使用的一次性设备是否会释放双酚，以及在常规条件下连续使用多个一次性设备时是否会累积双酚？双酚的提取遵循常规使用条件（温度和持续时间）。采用在线固相萃取/液相色谱/质谱方法测定了 10 种双酚的浓度。结果从 100 毫米培养皿（32 ± 20 pg）和高安全性精子吸管（3 ± 1 pg）中一致定量了双酚 S（BPS）。此外，从不含杀精剂的避孕套中也能稳定地定量检测到双酚 S 和双酚 A（BPA）（分别为 95 ± 78 和 83 ± 49 pg）。单独检测一次性装置时，没有检测到其他双酚。当组合使用一次性装置时，在 13 个一次性装置的组合中检测到双酚 A 和双酚 BPS（分别为 46 ± 16 pg 和 43 ± 32 pg）。在模拟精子收集、精子制备和冷冻的 14 种一次性装置组合中（10 ± 4 pg），以及模拟卵母细胞提取的 17 种一次性装置组合中（37 ± 22 pg），双酚 A 和双酚 B 的定量一致。

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引用次数: 0

Is there an association between the ‘October 7th’ events and IVF outcomes in Israel? 在以色列，"10 月 7 日 "事件与试管婴儿结果之间有关联吗？

IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online

Pub Date : 2024-08-23 DOI: 10.1016/j.rbmo.2024.104429

Einav Kadour-Peero , Chen Shkolnik , Janan Jadon , Rabea Bahous , Yael Gonen , Michal Silberman , Ilan Calderon , Hadar Avraham , Samer Khoury

Research question

Is there an association between the ‘October 7th’ 2023 events and IVF outcomes in Israel?

Design

in this retrospective cohort study the study group was women treated during the 4 weeks after ‘October 7th’, compared with a matched control group treated between 1 January 2021 and 6 October 2023. Each study group participant was matched with three separate control subjects of the same female age at oocyte retrieval, with a matching body mass index (BMI), IVF cycle number and ovarian reserve parameters.

Results

The study included 580 patients. The 145 women who underwent oocyte retrieval or frozen embryo transfer after 7 October 2023 were compared with 435 women treated before 7 October. The mean age of the study group was 35.6 ± 5.9 years, and the mean BMI 25.01 ± 5.60 kg/m². Pregnancy rates per embryo transfer were similar between the groups (22.4% versus 30.5%, P = 0.28 in fresh cycles; 16.9% versus 27.1%, P = 0.12 in frozen embryo transfers). Among women who underwent oocyte retrieval there were no differences in the ovarian stimulation protocols (P = 0.95), number of oocytes retrieved (P = 0.16) or number of fertilized oocytes (P = 0.34). There was no difference in the rate of good-quality embryos between groups (P = 0.053). Among women who underwent frozen embryo transfers, during the study period more patients had natural cycles (52.5% versus 24%, P < 0.001), and there was no difference in maximal endometrial thickness before embryo transfer (P = 0.33).

Conclusions

It appears that there is no association between the ‘October 7’ events and IVF outcomes.

研究问题：在以色列，2023 年 "10 月 7 日 "事件与试管婴儿结果之间是否存在关联？设计：在这项回顾性队列研究中，研究组是在 "10 月 7 日 "之后 4 周内接受治疗的女性，与在 2021 年 1 月 1 日至 2023 年 10 月 6 日期间接受治疗的匹配对照组进行比较。每个研究组的参与者都与三个独立的对照组受试者进行了配对，这些受试者在取卵时的女性年龄相同，体重指数（BMI）、试管婴儿周期数和卵巢储备参数也相匹配：研究包括 580 名患者。在 2023 年 10 月 7 日之后进行了取卵或冷冻胚胎移植的 145 名女性与 10 月 7 日之前接受治疗的 435 名女性进行了比较。研究组的平均年龄为 35.6 ± 5.9 岁，平均体重指数为 25.01 ± 5.60 kg/m2。两组每次胚胎移植的妊娠率相似（新鲜周期为 22.4% 对 30.5%，P = 0.28；冷冻胚胎移植为 16.9% 对 27.1%，P = 0.12）。在进行卵母细胞提取的妇女中，卵巢刺激方案（P = 0.95）、提取的卵母细胞数（P = 0.16）或受精卵母细胞数（P = 0.34）均无差异。各组之间的优质胚胎率没有差异（P = 0.053）。在接受冷冻胚胎移植的妇女中，研究期间自然周期的患者较多（52.5% 对 24%，P < 0.001），胚胎移植前最大子宫内膜厚度没有差异（P = 0.33）：结论："10 月 7 日 "事件与试管婴儿结果之间似乎没有关联。

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引用次数: 0

Inside Front Cover - Affiliations and First page of TOC 封面内页 - 隶属机构和目录首页

IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online

Pub Date : 2024-08-22 DOI: 10.1016/j.rbmo.2024.104387

引用次数: 0

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