Pub Date : 2024-02-20DOI: 10.52711/0974-360x.2024.00121
Hisham M. Elhalabi, Amr A. El- Waseif, Dina E El-Ghwas
In this study Chitosan nanoparticles was characterized usingUV spectrophotometry, FT-IR, Transmission electron microscopy, and X-Ray diffraction. The composition of Moringa oleifera of ethanolic extract was analyzed using GC-Mass.The antimicrobial, anti-inflammatory, and cytotoxicity of Chitosan-Moringa composite, H2CaO2 nanoparticles, Ca(OH)2, and Moringa oleifera of ethanol extract as an intra-canal medicament in vitro were also investigated. Results of our research summarized that; The UV of chitosan nanoparticles range from 280 to 300 nm. The FT-IR results confirm the presence of a broad and powerful band at 3442 cm1, 1636 cm1, and 1052 cm1 all band confirm the presence of the native chitosan. The X-Ray diffraction proved three strong characteristic peaks indicating crystallinity of chitosan nanoparticles chains. The TEM of Chitosan nanoparticles size was between 76.61 – 126.91nm and the shape was less spherical with slightly wrinkled surface. The major chemical compounds in Moringa ethanol extract by GC-Mass were detected. Also, the antimicrobial activity of Moringa extracts proved that ethanol extract had the highest zone of inhibition. The antimicrobial activity of Moringa-chitosan composite had the highest antimicrobial activity followed by H2CaO2 nanoparticles against dental pathogens. The anti-inflammatory effects on HRBC hemolysis at concentration 100μg/mL Ca (OH)2 gave the best lower production than the positive control Stander Indo meth followed by H2CaO2 nanoparticles, Moringa ethanol extract, and finally Chitosan-Moringa composite. The MTT assay against OEC have been showed that, Ca (OH)2 is the most cytotoxic with the lowest IC50 followed by Moringa ethanol extract, Chitosan-Moringa composite, and finally H2CaO2 nanoparticles.
{"title":"Assessment of Anti-inflammatory, Antimicrobial and Cytotoxicity of Chitosan-Moringa Composite and Calcium Hydroxide Nanoparticles as an intra-canal medicament in vitro","authors":"Hisham M. Elhalabi, Amr A. El- Waseif, Dina E El-Ghwas","doi":"10.52711/0974-360x.2024.00121","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00121","url":null,"abstract":"In this study Chitosan nanoparticles was characterized usingUV spectrophotometry, FT-IR, Transmission electron microscopy, and X-Ray diffraction. The composition of Moringa oleifera of ethanolic extract was analyzed using GC-Mass.The antimicrobial, anti-inflammatory, and cytotoxicity of Chitosan-Moringa composite, H2CaO2 nanoparticles, Ca(OH)2, and Moringa oleifera of ethanol extract as an intra-canal medicament in vitro were also investigated. Results of our research summarized that; The UV of chitosan nanoparticles range from 280 to 300 nm. The FT-IR results confirm the presence of a broad and powerful band at 3442 cm1, 1636 cm1, and 1052 cm1 all band confirm the presence of the native chitosan. The X-Ray diffraction proved three strong characteristic peaks indicating crystallinity of chitosan nanoparticles chains. The TEM of Chitosan nanoparticles size was between 76.61 – 126.91nm and the shape was less spherical with slightly wrinkled surface. The major chemical compounds in Moringa ethanol extract by GC-Mass were detected. Also, the antimicrobial activity of Moringa extracts proved that ethanol extract had the highest zone of inhibition. The antimicrobial activity of Moringa-chitosan composite had the highest antimicrobial activity followed by H2CaO2 nanoparticles against dental pathogens. The anti-inflammatory effects on HRBC hemolysis at concentration 100μg/mL Ca (OH)2 gave the best lower production than the positive control Stander Indo meth followed by H2CaO2 nanoparticles, Moringa ethanol extract, and finally Chitosan-Moringa composite. The MTT assay against OEC have been showed that, Ca (OH)2 is the most cytotoxic with the lowest IC50 followed by Moringa ethanol extract, Chitosan-Moringa composite, and finally H2CaO2 nanoparticles.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"989 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140446011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-20DOI: 10.52711/0974-360x.2024.00101
Herniyati Herniyati, Happy Harmono, Adiyatmoko Nur Rahman, Ratna Widyawati
Background: Orthodontic treatment is a treatment to correct malocclusion. The periodontal ligament plays an important role in preventing recurrence after orthodontic treatment. Fibroblast cells play a role in the regeneration of the periodontal ligament to maintain the stability of orthodontic treatment. Fibroblast cells play a role in the formation of collagen fibers that will connect teeth to bones. TGFß1 is a growth factor that plays a role in inducing the proliferation and transformation of fibroblasts into myofibroblasts and stimulates the accumulation of matrix proteins, including laminin, collagen 1 and 3, and fibronectin. Orthodontic treatment takes a long time so we need a method to speed it up. Belimbingwuluh leaf extract (Averrhoa bilimbi L.) contains antioxidants and anti-inflammatories which are expected to increase the number of fibroblasts and the density of collagen fibers. Objective : To analyze the potential of belimbingwuluh leaf extract in increasing the number of fibroblasts, TGFß1 expression and collagen fiber density during orthodontic tooth movement. Method: using 36 rats divided into 6 groups. The control group was fitted with orthodontic appliances without extracts, and the treatment group was fitted with orthodontic appliances and starfruit leaf extract for 7 days, 14 days and 21 days. An orthodontic appliance in the form of a closed coil spring Ni-Ti was installed to move the maxillary first molars mesially with orthodontic mechanical force. Histological preparations were made on M1 RA teeth with their periodontal ligament, then the tissue was stained with HE to observe fibroblasts, and Trichome Mallory staining for collagen fiber density and immunohistochemical staining to examine TGFß1 expression. Observation of fibroblasts and TGFß1 expression using a microscope and collagen density were observed using a microscope using the Adobe CS 6.0 application. Results: showed a significant increase in the number of fibroblasts, TGFß1 expression and density of collagen fibers in the 7, 14 and 21 day treatment group compared to the control group (p<0.05). Conclusion: BelimbingWuluh leaf extract increases fibroblasts, TGFß1 expression and collagen fiber density in the periodontal ligament during orthodontic tooth movement.
{"title":"Potential Analysis of Belimbing Wuluh (Averhoe bilimbi L.) leaf extract on increasing Fibroblasts, TGFß1 expression and density of Collagen fibers in the Periodontal ligament during Orthodontic Tooth Movement","authors":"Herniyati Herniyati, Happy Harmono, Adiyatmoko Nur Rahman, Ratna Widyawati","doi":"10.52711/0974-360x.2024.00101","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00101","url":null,"abstract":"Background: Orthodontic treatment is a treatment to correct malocclusion. The periodontal ligament plays an important role in preventing recurrence after orthodontic treatment. Fibroblast cells play a role in the regeneration of the periodontal ligament to maintain the stability of orthodontic treatment. Fibroblast cells play a role in the formation of collagen fibers that will connect teeth to bones. TGFß1 is a growth factor that plays a role in inducing the proliferation and transformation of fibroblasts into myofibroblasts and stimulates the accumulation of matrix proteins, including laminin, collagen 1 and 3, and fibronectin. Orthodontic treatment takes a long time so we need a method to speed it up. Belimbingwuluh leaf extract (Averrhoa bilimbi L.) contains antioxidants and anti-inflammatories which are expected to increase the number of fibroblasts and the density of collagen fibers. Objective : To analyze the potential of belimbingwuluh leaf extract in increasing the number of fibroblasts, TGFß1 expression and collagen fiber density during orthodontic tooth movement. Method: using 36 rats divided into 6 groups. The control group was fitted with orthodontic appliances without extracts, and the treatment group was fitted with orthodontic appliances and starfruit leaf extract for 7 days, 14 days and 21 days. An orthodontic appliance in the form of a closed coil spring Ni-Ti was installed to move the maxillary first molars mesially with orthodontic mechanical force. Histological preparations were made on M1 RA teeth with their periodontal ligament, then the tissue was stained with HE to observe fibroblasts, and Trichome Mallory staining for collagen fiber density and immunohistochemical staining to examine TGFß1 expression. Observation of fibroblasts and TGFß1 expression using a microscope and collagen density were observed using a microscope using the Adobe CS 6.0 application. Results: showed a significant increase in the number of fibroblasts, TGFß1 expression and density of collagen fibers in the 7, 14 and 21 day treatment group compared to the control group (p<0.05). Conclusion: BelimbingWuluh leaf extract increases fibroblasts, TGFß1 expression and collagen fiber density in the periodontal ligament during orthodontic tooth movement.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"590 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140446612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-20DOI: 10.52711/0974-360x.2024.00131
Ni Nyoman Yuliani, S. Siswandono, T. Erawati, Jefrin Sambara, Y. Korassa, Sandeep Poddar
A research has been conducted on the Sunscreen Activity Test of Bougainvillae spectabilis Willd (Bougainvillae spectabilis Willd) Flower Ethanol Extract. Bougainvillae spectabilis Willd is a shrubby and thorny plant. The flowers are collected into three, belonging to the Nytaginaceae family, which is efficacious as a sunscreen because they contain active compounds such as flavonoids and phenolics, which are polyphenolic compounds. The researchers wanted to know whether or not Bougainvillea spectabilis Willd's ethanolic extract had any anti-inflammatory or sun protection properties, so they measured things like the SPF value, the amount of erythema transmitted (Te%), and the amount of pigmentation transmitted (Tp%). Bougainvillae spectabilis Willd was extracted by maceration for 3 days and remaceration for 2 days using ethanol as the solvent, and the yield percentage was 12.70%. Furthermore, phytochemical screening and an in vitro Sunscreen Activity Test were carried out using the UV-Vis spectrophotometer. The results showed that the ethanolic extract of Bougainvillae spectabilis Willd contained flavonoids, alkaloids, saponins, tannins, and phenolics. Based on the sunscreen activity test, the ethanol extract made in 4 concentrations showed maximum sunscreen activity at SPF values with concentrations of 600 ppm (20,715), 800 ppm (64,367).
{"title":"Bougenville Flower (Bougainvillae spectabilis Willd) Extract (In Vitro) Activity Test as Sunscreen","authors":"Ni Nyoman Yuliani, S. Siswandono, T. Erawati, Jefrin Sambara, Y. Korassa, Sandeep Poddar","doi":"10.52711/0974-360x.2024.00131","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00131","url":null,"abstract":"A research has been conducted on the Sunscreen Activity Test of Bougainvillae spectabilis Willd (Bougainvillae spectabilis Willd) Flower Ethanol Extract. Bougainvillae spectabilis Willd is a shrubby and thorny plant. The flowers are collected into three, belonging to the Nytaginaceae family, which is efficacious as a sunscreen because they contain active compounds such as flavonoids and phenolics, which are polyphenolic compounds. The researchers wanted to know whether or not Bougainvillea spectabilis Willd's ethanolic extract had any anti-inflammatory or sun protection properties, so they measured things like the SPF value, the amount of erythema transmitted (Te%), and the amount of pigmentation transmitted (Tp%). Bougainvillae spectabilis Willd was extracted by maceration for 3 days and remaceration for 2 days using ethanol as the solvent, and the yield percentage was 12.70%. Furthermore, phytochemical screening and an in vitro Sunscreen Activity Test were carried out using the UV-Vis spectrophotometer. The results showed that the ethanolic extract of Bougainvillae spectabilis Willd contained flavonoids, alkaloids, saponins, tannins, and phenolics. Based on the sunscreen activity test, the ethanol extract made in 4 concentrations showed maximum sunscreen activity at SPF values with concentrations of 600 ppm (20,715), 800 ppm (64,367).","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"510 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140446758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-20DOI: 10.52711/0974-360x.2024.00127
Mohammad Ozair Sekandari, Amin Ghasem Begloo, Khalil Alimohammadzadeh
Objective: This study aims to investigate the role of pharmacists in delivering pharmaceutical services within selected government hospitals in Kabul, utilizing the SERVQUAL model. Methods: A quantitative research design was employed, utilizing a sample population of 338 patients who were hospitalized in the selected hospitals. A structured questionnaire was used to collect data on socio-demographic characteristics and perceptions of pharmaceutical services. The data were analyzed using the linear regression test to examine the relationships between the role of pharmacists and the combined indices. Results: he results indicated a significant positive relationship between the role of pharmacists and the combined indices of tangible factors, reliability and credibility, responsiveness, reassurance, and empathy (R = 0.642, R-squared = 0.412, Adjusted R-squared = 0.403, p < 0.001). Tangible factors, reliability and credibility, and empathy demonstrated the most substantial influence on the pharmacists' role. Conclusion: The results demonstrate that patients in selected hospitals within Kabul are content with the quality of services provided. Based on the SERVQUAL model, patient satisfaction with service quality is achieved across five dimensions: tangible factors, reliability, responsiveness, assurance, and empathy. Notably, the reliability variable garners the highest satisfaction level, while the empathy variable receives the lowest. These findings emphasize the need for targeted efforts to enhance empathy, communication, and the overall quality of pharmaceutical services delivered by pharmacists in selected hospitals within Kabul.
{"title":"The Role of Pharmacists in Providing Pharmaceutical Services in Selected Government Hospitals in Kabul","authors":"Mohammad Ozair Sekandari, Amin Ghasem Begloo, Khalil Alimohammadzadeh","doi":"10.52711/0974-360x.2024.00127","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00127","url":null,"abstract":"Objective: This study aims to investigate the role of pharmacists in delivering pharmaceutical services within selected government hospitals in Kabul, utilizing the SERVQUAL model. Methods: A quantitative research design was employed, utilizing a sample population of 338 patients who were hospitalized in the selected hospitals. A structured questionnaire was used to collect data on socio-demographic characteristics and perceptions of pharmaceutical services. The data were analyzed using the linear regression test to examine the relationships between the role of pharmacists and the combined indices. Results: he results indicated a significant positive relationship between the role of pharmacists and the combined indices of tangible factors, reliability and credibility, responsiveness, reassurance, and empathy (R = 0.642, R-squared = 0.412, Adjusted R-squared = 0.403, p < 0.001). Tangible factors, reliability and credibility, and empathy demonstrated the most substantial influence on the pharmacists' role. Conclusion: The results demonstrate that patients in selected hospitals within Kabul are content with the quality of services provided. Based on the SERVQUAL model, patient satisfaction with service quality is achieved across five dimensions: tangible factors, reliability, responsiveness, assurance, and empathy. Notably, the reliability variable garners the highest satisfaction level, while the empathy variable receives the lowest. These findings emphasize the need for targeted efforts to enhance empathy, communication, and the overall quality of pharmaceutical services delivered by pharmacists in selected hospitals within Kabul.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"333 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-20DOI: 10.52711/0974-360x.2024.00106
Prabhu Manickam Natarajan, Mohamed Abdullah Jaber, U. V. Mds, S. Bhuminathan, M. S. Nandini, Vijay Bhavrao Desai, Mohammad Kashif Shafiq Khot
Major regenerative therapies for periodontium are guided tissue regeneration (GTR) and bone grafting. Grafting the bone with hydroxyapatite is the current standard of care. Phytochemicals possess biological activity in animal physiological systems that offers affordable standard of care to the patients. In this context, osteogenic phytochemicals are reported in the literature. However, there is a lacuna in the literature in comparative efficacy of these phytochemicals. In this review three phytochemicals are compared – viz.β-sitosterol, Genistein and Emodin. Having proven by the previous investigators that β-sitosterol, Genitein and Emodin have effective osteogenic action when applied locally, it would be prudent to analyse the synergistic action for better osteogenesis. In principle, synergistic action is advantageous for both intensity and duration of action. As these molecules act at cellular level and have action on wide range of cells, it is necessary to deliver them locally with a proper scaffold or instrument. Effective combinations of these molecules can be incorporated with periodontal regenerative materials (GTR) to shift the balance towards regeneration of periodontium.
{"title":"Phytochemicals in Periodontal Bone Regeneration","authors":"Prabhu Manickam Natarajan, Mohamed Abdullah Jaber, U. V. Mds, S. Bhuminathan, M. S. Nandini, Vijay Bhavrao Desai, Mohammad Kashif Shafiq Khot","doi":"10.52711/0974-360x.2024.00106","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00106","url":null,"abstract":"Major regenerative therapies for periodontium are guided tissue regeneration (GTR) and bone grafting. Grafting the bone with hydroxyapatite is the current standard of care. Phytochemicals possess biological activity in animal physiological systems that offers affordable standard of care to the patients. In this context, osteogenic phytochemicals are reported in the literature. However, there is a lacuna in the literature in comparative efficacy of these phytochemicals. In this review three phytochemicals are compared – viz.β-sitosterol, Genistein and Emodin. Having proven by the previous investigators that β-sitosterol, Genitein and Emodin have effective osteogenic action when applied locally, it would be prudent to analyse the synergistic action for better osteogenesis. In principle, synergistic action is advantageous for both intensity and duration of action. As these molecules act at cellular level and have action on wide range of cells, it is necessary to deliver them locally with a proper scaffold or instrument. Effective combinations of these molecules can be incorporated with periodontal regenerative materials (GTR) to shift the balance towards regeneration of periodontium.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"41 36","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-20DOI: 10.52711/0974-360x.2024.00115
Saurav R. Dunbale, Deelip V. Derle, Ashlesha A. Wakchaure, Ashwini A. Amrutkar, Amol V. More
Rivaroxaban is andirect acting oralanticoagulant and factor Xa inhibitor. A simple, selective, precise and rapid RP-HPLC method for estimation of Rivaroxaban (RIVA) in human blood plasma was developed and validated. The sample spike in plasma was extracted using liquid liquid extraction were extracted with the organic solvent ethyl acetate as organic solvent. Apixaban as an internal standard. The compounds were analysed by Agilent HPLC was used with control panel software using UV detector on a Inertsil ODS (250mm x 4.6mm ID;5μ) column with an Flow rate of 1.2mL/min, an isocratic mobile phase consisting of 0.02M Ammonium acetate buffer: Acetonitrile (70:30%v/v). Different sample pre-treatment techniques were evaluated, but Liquid Liquid extraction was found to be satisfactory, with good recovery values of 93.70% for RIVA. The developed method is validated by ICHM10 and USFDA guidelines over the concentration range of 5.00 to 200.00 ng/ml in human blood plasma with R² =0.9993. Within-day precisions and accuracy for RIVA were found in 0.36% to 4.73% and 92.58% to101.82% respectively. The validated RP-HPLC method has been used successfully for both preliminary pharmacokinetic studies and therapeutic drug monitoring
{"title":"Development and Validation of Bioanalytical Method for Estimation of Rivaroxaban using RP-HPLC with Liquid liquid extraction in Human Blood Plasma and its application in Bioequivalence Study","authors":"Saurav R. Dunbale, Deelip V. Derle, Ashlesha A. Wakchaure, Ashwini A. Amrutkar, Amol V. More","doi":"10.52711/0974-360x.2024.00115","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00115","url":null,"abstract":"Rivaroxaban is andirect acting oralanticoagulant and factor Xa inhibitor. A simple, selective, precise and rapid RP-HPLC method for estimation of Rivaroxaban (RIVA) in human blood plasma was developed and validated. The sample spike in plasma was extracted using liquid liquid extraction were extracted with the organic solvent ethyl acetate as organic solvent. Apixaban as an internal standard. The compounds were analysed by Agilent HPLC was used with control panel software using UV detector on a Inertsil ODS (250mm x 4.6mm ID;5μ) column with an Flow rate of 1.2mL/min, an isocratic mobile phase consisting of 0.02M Ammonium acetate buffer: Acetonitrile (70:30%v/v). Different sample pre-treatment techniques were evaluated, but Liquid Liquid extraction was found to be satisfactory, with good recovery values of 93.70% for RIVA. The developed method is validated by ICHM10 and USFDA guidelines over the concentration range of 5.00 to 200.00 ng/ml in human blood plasma with R² =0.9993. Within-day precisions and accuracy for RIVA were found in 0.36% to 4.73% and 92.58% to101.82% respectively. The validated RP-HPLC method has been used successfully for both preliminary pharmacokinetic studies and therapeutic drug monitoring","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"1095 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140446076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hyperlipidemia is a risk factor of cardiovascular disorder in diabetes mellitus. Lack of insulin in diabetes mellitus can increase lipolysis which leads to an increased free fatty acid that also increases total cholesterol level and LDL (Low-Density Lipoprotein). This study aims to determine the effect of Muntingia calabura leaf extract on the level of total cholesterol (TC), triglycerides (TG), LDL, and High Density Lipoproteins (HDL) of alloxan-induced male white mice. Thirty male mice were separated into five groups: negative control (NC) without alloxan and extract, diabetes control (DC) with only alloxan, treatment 1 (T1) with alloxan and 100mg/kgBW of M. calabura leaf extract, treatment 2 (T2) with alloxan and 300 mg/kgBW of M. calabura leaves extract, and positive control (PC) with alloxan and 600 µg/kgBW of glibenclamide. One-Way Anova demonstrated that the administration of glibenclamide and M. calabura effectively reduce TC, TG, LDL and increase HDL compared to the diabetes control group (P<0.05) and the most effective dose M. calabura to decrease TC, TG, LDL, and to increase HDL were 300 mg/kgBW. Extraction using 96% ethanol of M. calabura leaf affects the reduction of TC, TG, LDL, and the increase of HDL in the blood and it can be developed as alternative of antihyperlipidemia drugs.
{"title":"Effect of Ethanol Extract from Kersen Leaves (Muntingia calabura L.) on Changes in Blood Factors Total Cholesterol, Triglycerides, LDL and HDL in Hyperlipidemia Mice","authors":"Tridiganita Intan Solikhah, Gahastanira Permata Solikhah","doi":"10.52711/0974-360x.2024.00112","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00112","url":null,"abstract":"Hyperlipidemia is a risk factor of cardiovascular disorder in diabetes mellitus. Lack of insulin in diabetes mellitus can increase lipolysis which leads to an increased free fatty acid that also increases total cholesterol level and LDL (Low-Density Lipoprotein). This study aims to determine the effect of Muntingia calabura leaf extract on the level of total cholesterol (TC), triglycerides (TG), LDL, and High Density Lipoproteins (HDL) of alloxan-induced male white mice. Thirty male mice were separated into five groups: negative control (NC) without alloxan and extract, diabetes control (DC) with only alloxan, treatment 1 (T1) with alloxan and 100mg/kgBW of M. calabura leaf extract, treatment 2 (T2) with alloxan and 300 mg/kgBW of M. calabura leaves extract, and positive control (PC) with alloxan and 600 µg/kgBW of glibenclamide. One-Way Anova demonstrated that the administration of glibenclamide and M. calabura effectively reduce TC, TG, LDL and increase HDL compared to the diabetes control group (P<0.05) and the most effective dose M. calabura to decrease TC, TG, LDL, and to increase HDL were 300 mg/kgBW. Extraction using 96% ethanol of M. calabura leaf affects the reduction of TC, TG, LDL, and the increase of HDL in the blood and it can be developed as alternative of antihyperlipidemia drugs.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"740 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140446415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-20DOI: 10.52711/0974-360x.2024.00132
Michael Ganda Wijaya, C. Prahasanti, Bambang Dwi Laksono, Westy Agrawanty, Banun Kusumawardhani, Maria Jessica Anggakusuma
Surgery is required to restore bone loss brought on by regenerative periodontal diseases while retaining the patient's aesthetics. The bone deficits caused by periodontal disease have been repaired using a variety of transplant materials. One of the graft materials used is dentin since it resembles bone in terms of both organic and inorganic components. In order to evaluate the viability of dental grafts, this study intended to count the osteoblast cells that were still alive after a specific therapy. Osteoblast cell cultures in 42 well plates were employed in this work. The 42 well plate cell cultures were separated into seven groups for 24hour examinations and seven groups for 48 hour examinations in order to examine the cells using the MTT assay. Each group contained control cells, control media devoid of cells, and the treatment group, which received tooth transplant at doses of 8, 4, 2, 1, and 0.5mg/mL. Using an ELISA reader with a 595nm wavelength, the optical density of these cells was used to determine the viability of the cells. There are more than 50% of osteoblast cells in all concentrations, which is indicated by the number of these cells. The Shapiro-Wilk, Levene, and Oneway Anova tests were performed to assess the normality, uniformity, and degree of group differences in the data. This study demonstrates the biocompatibility of the tooth graft and the osteoblast cells.
{"title":"Viability Test of Hydroxyapatite Tooth Graft on Osteoblast cell culture","authors":"Michael Ganda Wijaya, C. Prahasanti, Bambang Dwi Laksono, Westy Agrawanty, Banun Kusumawardhani, Maria Jessica Anggakusuma","doi":"10.52711/0974-360x.2024.00132","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00132","url":null,"abstract":"Surgery is required to restore bone loss brought on by regenerative periodontal diseases while retaining the patient's aesthetics. The bone deficits caused by periodontal disease have been repaired using a variety of transplant materials. One of the graft materials used is dentin since it resembles bone in terms of both organic and inorganic components. In order to evaluate the viability of dental grafts, this study intended to count the osteoblast cells that were still alive after a specific therapy. Osteoblast cell cultures in 42 well plates were employed in this work. The 42 well plate cell cultures were separated into seven groups for 24hour examinations and seven groups for 48 hour examinations in order to examine the cells using the MTT assay. Each group contained control cells, control media devoid of cells, and the treatment group, which received tooth transplant at doses of 8, 4, 2, 1, and 0.5mg/mL. Using an ELISA reader with a 595nm wavelength, the optical density of these cells was used to determine the viability of the cells. There are more than 50% of osteoblast cells in all concentrations, which is indicated by the number of these cells. The Shapiro-Wilk, Levene, and Oneway Anova tests were performed to assess the normality, uniformity, and degree of group differences in the data. This study demonstrates the biocompatibility of the tooth graft and the osteoblast cells.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"524 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140446920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-20DOI: 10.52711/0974-360x.2024.00134
Nilima Navnath Khakal, Nagesh Hanmantrao Aloorkar
A “drug delivery system” should be able to reduce toxicity and improve therapeutic benefits. The present investigation aimed to provide an approach for the solubility and bioavailability enhancement by a novel polymer platform Drug delivery system. Platform technology contains a polymeric system with a release modulator and can accommodate drugs with common physicochemical /therapeutic properties with minimal changes. Pioglitazone, BCS class II drug results in sub-therapeutic plasma drug levels which can cause failure in therapeutic response. When it comes to make PIO dissolved and soluble, microwave assisted ball milling technique was followed. Chitosan and neusiline US2 were used to prepare solid dispersion forming ternary complexation. Optimization of solid dispersion of ternary complexation, a “32 level full factorial design with Design Expert Software version 12” had been used. Pioglitazone–CH-neusiline systems helped in marked development of solubility of initial medicinal water, drug dissolution and drug stability. According to the “FTIR, DSC, and XRPD studies, PIO-CH-NS complexes could be prepared by microwave-assisted milling technology has formed stable crystalline in a ternary complex system. A novel polymer platform technique increases bioavailability, enhancing the therapeutic effect while reducing the toxicity of drug molecules with improving patient compliance.
一种 "给药系统 "应能减少毒性并提高治疗效果。本研究旨在提供一种通过新型聚合物平台给药系统提高溶解度和生物利用度的方法。该平台技术包含一个带有释放调节剂的聚合物系统，能以最小的变化容纳具有共同理化/治疗特性的药物。吡格列酮是 BCS II 类药物，其血浆药物浓度低于治疗水平，会导致治疗反应失败。为了使 PIO 溶解，我们采用了微波辅助球磨技术。壳聚糖和新西林 US2 被用来制备形成三元复合物的固体分散体。优化三元复合物的固体分散体时，采用了 "32 级全因子设计，设计专家软件版本 12"。Pioglitazone-CH-neusiline 系统有助于显著提高初始药水的溶解度、药物溶解度和药物稳定性。根据 "傅立叶变换红外光谱（FTIR）、电化学稳定性（DSC）和 XRPD 研究"，通过微波辅助研磨技术制备的 PIO-CH-NS 复合物在三元复合物体系中形成了稳定的晶体。新型聚合物平台技术提高了生物利用度，增强了治疗效果，同时降低了药物分子的毒性，改善了患者的依从性。
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Pub Date : 2024-02-20DOI: 10.52711/0974-360x.2024.00094
Elsa Fitria Apriani, S. Shiyan, Dwi Hardestyariki, Viva Starlista, Anggitia Laras Sari
Clindamycin HCl has anti-acne properties because it can inhibit the growth of Propionibacterium acnes (P. acnes) bacteria. However, the bioavailability of clindamycin HCl is less than 13% of the given dose, so it needs to be developed in the form of ethosomes to increase its bioavailability. This study aimed to create a clindamycin HCl ethosomal gel preparation with varying hydroxypropyl methylcellulose (HPMC) and carbopol-940 as a gelling agent using a factorial design to obtain 4 formulas. The concentrations of HPMC and carbopol 940 used were 0.5% and 1%. The optimum formula was determined based on the pH response, viscosity, spreadability, and adhesion of the clindamycin HCl ethosomal gel preparation. Based on the factorial design analysis, HPMC and Carbopol-940 have an influence on the pH response and adhesion, while the interaction between HPMC and Carbopol-940 influences the spreadability and viscosity responses. The optimum formula was obtained with a desirability value of 0.994 at a concentration of HPMC was 1%, and Carbopol 940 was 0.5%. The minimum inhibitory concentration of the optimum clindamycin HCl ethosomal gel was 60 µg/mL. So, the clindamycin HCl ethosomal gel had a strong antibacterial activity.
{"title":"Optimization of Hydroxypropyl Methylcellulose (HPMC) and Carbopol 940 in Clindamycin HCl Ethosomal Gel as Anti-acne","authors":"Elsa Fitria Apriani, S. Shiyan, Dwi Hardestyariki, Viva Starlista, Anggitia Laras Sari","doi":"10.52711/0974-360x.2024.00094","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00094","url":null,"abstract":"Clindamycin HCl has anti-acne properties because it can inhibit the growth of Propionibacterium acnes (P. acnes) bacteria. However, the bioavailability of clindamycin HCl is less than 13% of the given dose, so it needs to be developed in the form of ethosomes to increase its bioavailability. This study aimed to create a clindamycin HCl ethosomal gel preparation with varying hydroxypropyl methylcellulose (HPMC) and carbopol-940 as a gelling agent using a factorial design to obtain 4 formulas. The concentrations of HPMC and carbopol 940 used were 0.5% and 1%. The optimum formula was determined based on the pH response, viscosity, spreadability, and adhesion of the clindamycin HCl ethosomal gel preparation. Based on the factorial design analysis, HPMC and Carbopol-940 have an influence on the pH response and adhesion, while the interaction between HPMC and Carbopol-940 influences the spreadability and viscosity responses. The optimum formula was obtained with a desirability value of 0.994 at a concentration of HPMC was 1%, and Carbopol 940 was 0.5%. The minimum inhibitory concentration of the optimum clindamycin HCl ethosomal gel was 60 µg/mL. So, the clindamycin HCl ethosomal gel had a strong antibacterial activity.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"365 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140447952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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