The industrialization of agriculture has gradually taken place in Colombia, because of the need to optimize the time and effort required for the processes involved from growing fruits and vegetables to their distribution and commercialization, whether they are meant for exportation or commercialization within the country. Fruit classification is a very important process before they are being taken out as a final product, since predetermined guidelines issued by regulatory entities that define the quality of the harvested product must be followed. The authors propose a low-cost prototype for the automatic classification of bananas according to the NTC 1190 standard (Colombian normative), using a convolutional neural network (CNN) of MobileNetV2 architecture trained through transfer learning and implemented in a Raspberry Pi 3B+ with a camera to monitor the specimens and an easy interface for interaction with the user, as well as a case designed to contain the hardware and allow access to its ports in the most compact way possible. The datasets utilized in this work for training, validation, and testing, consists of images taken from two free access fruit database and others acquired by the researchers. The achieved precision is 87 %, enough to ensure reliability and low computational cost.
{"title":"Automatic Identification of banana quality with Deep Neural Network Classification (DNN)","authors":"Deyner Julian Navarro Ortiz, Silvia Alejandra Martinez Lopez","doi":"10.18682/cyt.vi22.4609","DOIUrl":"https://doi.org/10.18682/cyt.vi22.4609","url":null,"abstract":"The industrialization of agriculture has gradually taken place in Colombia, because of the need to optimize the time and effort required for the processes involved from growing fruits and vegetables to their distribution and commercialization, whether they are meant for exportation or commercialization within the country. Fruit classification is a very important process before they are being taken out as a final product, since predetermined guidelines issued by regulatory entities that define the quality of the harvested product must be followed. The authors propose a low-cost prototype for the automatic classification of bananas according to the NTC 1190 standard (Colombian normative), using a convolutional neural network (CNN) of MobileNetV2 architecture trained through transfer learning and implemented in a Raspberry Pi 3B+ with a camera to monitor the specimens and an easy interface for interaction with the user, as well as a case designed to contain the hardware and allow access to its ports in the most compact way possible. The datasets utilized in this work for training, validation, and testing, consists of images taken from two free access fruit database and others acquired by the researchers. The achieved precision is 87 %, enough to ensure reliability and low computational cost.","PeriodicalId":21243,"journal":{"name":"Revista de Ciencia y Tecnología","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84581429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Taccone, N. Oviedo, S. Montenegro, M. J. Ganiele, Melanie Melo, Luciana Quinteros, M. Artaza, G. Gonzalez, Luciana Abadie, S. Fabbro, Luciano Attorresi, Lucio M. E. Ponzoni
Este trabajo busca investigar la factibilidad de fabricación de placas de hormigón con plástico tipo polietileno tereftalato (PET) como sustituto parcial de la arena. Actualmente, el PET no es biodegradable y contribuye con la problemática ambiental de gestión de residuos, por su complejo reciclado y su extensa generación debida a su tendencia a utilizarse para contenedores de un solo uso. Para llevar a cabo la investigación, se evaluaron niveles de sustitución del 20% y 35%; y dos granulometrías de PET conseguidas a través de distintas cooperativas de reciclado. A estas variantes se les realizaron ensayos en fresco de densidad y trabajabilidad, y ensayos en seco de resistencia al fuego y aptitud para perforación. Los resultados experimentales demostraron que la sustitución óptima para utilizar en construcciones futuras corresponde a la del 20%, con una granulometría fina.
{"title":"Diseño y fabricación experimental de placas cemento-PET para ser utilizadas en la construcción de muros no portantes en ecoviviendas","authors":"V. Taccone, N. Oviedo, S. Montenegro, M. J. Ganiele, Melanie Melo, Luciana Quinteros, M. Artaza, G. Gonzalez, Luciana Abadie, S. Fabbro, Luciano Attorresi, Lucio M. E. Ponzoni","doi":"10.18682/cyt.vi22.7058","DOIUrl":"https://doi.org/10.18682/cyt.vi22.7058","url":null,"abstract":"Este trabajo busca investigar la factibilidad de fabricación de placas de hormigón con plástico tipo polietileno tereftalato (PET) como sustituto parcial de la arena. Actualmente, el PET no es biodegradable y contribuye con la problemática ambiental de gestión de residuos, por su complejo reciclado y su extensa generación debida a su tendencia a utilizarse para contenedores de un solo uso. Para llevar a cabo la investigación, se evaluaron niveles de sustitución del 20% y 35%; y dos granulometrías de PET conseguidas a través de distintas cooperativas de reciclado. A estas variantes se les realizaron ensayos en fresco de densidad y trabajabilidad, y ensayos en seco de resistencia al fuego y aptitud para perforación. Los resultados experimentales demostraron que la sustitución óptima para utilizar en construcciones futuras corresponde a la del 20%, con una granulometría fina.","PeriodicalId":21243,"journal":{"name":"Revista de Ciencia y Tecnología","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85764730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.36995/j.recyt.2022.38.003
Dalia M. Berbes Villalón, Laura Sánchez Jiménez, Manuel de la Iglesia Campos, María E. Díaz Aguirre, Tatiana Delgado Fernández
The Internet of Things (IoT) is a concept that has been gaining considerable popularity today. It attempts to represent everyday things that are connected to the internet, but in reality it is much more than that. The overall aim is to interconnect the physical with the digital world, as the physical world is measured by sensors which translates into actionable data, and also the data can be translated into commands to be executed by actuators. Currently, the number of designed IoT architectures has increased considerably as a result of different approaches, standards and use cases. This leads to difficulties in understanding, selecting and using these architectures. In this work, an IoT architecture based on the FIWARE platform is proposed with the aim of facilitating the development of smart cities. With the proposal made, it was possible to integrate the main elements to be considered in this technology, thus offering a basis that serves as a guide, both for developing IoT systems and for creating more specific architectures that respond to the particular characteristics of a given application.
{"title":"An IoT architecture for smart cities based on the FIWARE platform","authors":"Dalia M. Berbes Villalón, Laura Sánchez Jiménez, Manuel de la Iglesia Campos, María E. Díaz Aguirre, Tatiana Delgado Fernández","doi":"10.36995/j.recyt.2022.38.003","DOIUrl":"https://doi.org/10.36995/j.recyt.2022.38.003","url":null,"abstract":"The Internet of Things (IoT) is a concept that has been gaining considerable popularity today. It attempts to represent everyday things that are connected to the internet, but in reality it is much more than that. The overall aim is to interconnect the physical with the digital world, as the physical world is measured by sensors which translates into actionable data, and also the data can be translated into commands to be executed by actuators.\u0000Currently, the number of designed IoT architectures has increased considerably as a result of different approaches, standards and use cases. This leads to difficulties in understanding, selecting and using these architectures. In this work, an IoT architecture based on the FIWARE platform is proposed with the aim of facilitating the development of smart cities. With the proposal made, it was possible to integrate the main elements to be considered in this technology, thus offering a basis that serves as a guide, both for developing IoT systems and for creating more specific architectures that respond to the particular characteristics of a given application.","PeriodicalId":21243,"journal":{"name":"Revista de Ciencia y Tecnología","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89146240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.36995/j.recyt.2022.38.004
A. Cazzaniga, M. Brousse, R. A. Linares
Sorption characteristics are studied for their influence on the storage stability of dehydrated products and their effect on water vapour diffusion. In the present work, the adsorption curves of snacks with and without substitution of wheat flour by dehydrated cassava puree (PDM) were analysed using saturated salt solutions at 10, 30 and 50 ºC. Type III curves were obtained for the samples with PDM and type II for the control sample. Mathematical modelling was then performed using 8 different equations, obtaining the best fits for the Peleg (R2adjust =0.999-0.988), Ferro Fontan (R2adjust =0.998-0.970) and GAB (R2adjust =0.999-0.955) models. The values of the model constants in some cases turned out to be formulation and temperature dependent, and in general do not show a definite trend. This can be seen graphically as crossovers between the curves. This crossover of curves has been attributed to the increased exposure of hydrophilic groups due to increased molecular mobility at high humidity and occurrence of the glass transition in the system and/or endothermic dissolution of sugars.
{"title":"Evaluation of sorption isotherms in snacks with pregelatinized cassava","authors":"A. Cazzaniga, M. Brousse, R. A. Linares","doi":"10.36995/j.recyt.2022.38.004","DOIUrl":"https://doi.org/10.36995/j.recyt.2022.38.004","url":null,"abstract":"Sorption characteristics are studied for their influence on the storage stability of dehydrated products and their effect on water vapour diffusion. In the present work, the adsorption curves of snacks with and without substitution of wheat flour by dehydrated cassava puree (PDM) were analysed using saturated salt solutions at 10, 30 and 50 ºC.\u0000Type III curves were obtained for the samples with PDM and type II for the control sample. Mathematical modelling was then performed using 8 different equations, obtaining the best fits for the Peleg (R2adjust =0.999-0.988), Ferro Fontan (R2adjust =0.998-0.970) and GAB (R2adjust =0.999-0.955) models. The values of the model constants in some cases turned out to be formulation and temperature dependent, and in general do not show a definite trend.\u0000This can be seen graphically as crossovers between the curves. This crossover of curves has been attributed to the increased exposure of hydrophilic groups due to increased molecular mobility at high humidity and occurrence of the glass transition in the system and/or endothermic dissolution of sugars.","PeriodicalId":21243,"journal":{"name":"Revista de Ciencia y Tecnología","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76171104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.36995/j.recyt.2022.38.007
L. Celaya, D. Ferreyra, Eugenio Kolb, Nicolás Kolb Koslobsky
The aim of this study was to analyze the optimum temperature and percentage of ethanol to extract steviol glycosides (Stevioside and Rebaudioside A) from stevia leaves in a percolation extractor. The experiments were performed in broad ranges of binary solvent (0-100 % ethanol) and temperature (30–70 °C) in order to investigate their effects on steviol glycosides recovery and purity. The optimal extraction conditions, which gave a best steviol glycosides extraction kinetic were 70 °C and 35% of ethanol. In addition, the optimal extraction condition giving maximum purity was 70% of ethanol between 30 and 70°C.
{"title":"Extraction of Stevioside and Rebaudioside A from Stevia rebaudiana by percolation with ethanol-water solvents","authors":"L. Celaya, D. Ferreyra, Eugenio Kolb, Nicolás Kolb Koslobsky","doi":"10.36995/j.recyt.2022.38.007","DOIUrl":"https://doi.org/10.36995/j.recyt.2022.38.007","url":null,"abstract":"The aim of this study was to analyze the optimum temperature and percentage of ethanol to extract steviol glycosides (Stevioside and Rebaudioside A) from stevia leaves in a percolation extractor. The experiments were performed in broad ranges of binary solvent (0-100 % ethanol) and temperature (30–70 °C) in order to investigate their effects on steviol glycosides recovery and purity. The optimal extraction conditions, which gave a best steviol glycosides extraction kinetic were 70 °C and 35% of ethanol. In addition, the optimal extraction condition giving maximum purity was 70% of ethanol between 30 and 70°C.","PeriodicalId":21243,"journal":{"name":"Revista de Ciencia y Tecnología","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89908873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.36995/j.recyt.2022.38.005
C. G. Sambiasi, A. E. Pascual Barrera, M. Sambiasi
This paper reports the behaviour of solid urban waste generation in the Metropolitan Area of Misiones, during a period of seven days. The waste analysed came from collection routes by household sampling points determined according to the land use and socioeconomic level of the population in the study area. The methodology consisted of visualising the sample universe, selecting the household waste collection routes and then classifying and analysing their composition according to ASTM D5231-92 (2016). This strategy was considered valid, given that the habits and customs of the population are closely related to the socioeconomic levels that directly affect consumption and consequently the quality of waste. Organic matter (51.80%) was the most representative, followed by materials with recycling potential, such as plastic (13.90%), glass (7.90%), paper and cardboard (7.80%), metals/aluminium (2.20%) and tetrabrik (2.60%). The quality of MSW was not uniform between cities, with Garupá (61.50%) being the city that generated the most organic waste, followed by Posadas (57.50%) and ending with Candelaria (29.00%) with a marked decrease. As a limitation of the method, it was determined that the results are representative of each city and of the winter season.
{"title":"Urban Solid Waste Characterization of the Misiones Metropolitan Area","authors":"C. G. Sambiasi, A. E. Pascual Barrera, M. Sambiasi","doi":"10.36995/j.recyt.2022.38.005","DOIUrl":"https://doi.org/10.36995/j.recyt.2022.38.005","url":null,"abstract":"This paper reports the behaviour of solid urban waste generation in the Metropolitan Area of Misiones, during a period of seven days. The waste analysed came from collection routes by household sampling points determined according to the land use and socioeconomic level of the population in the study area. The methodology consisted of visualising the sample universe, selecting the household waste collection routes and then classifying and analysing their composition according to ASTM D5231-92 (2016). This strategy was considered valid, given that the habits and customs of the population are closely related to the socioeconomic levels that directly affect consumption and consequently the quality of waste. Organic matter (51.80%) was the most representative, followed by materials with recycling potential, such as plastic (13.90%), glass (7.90%), paper and cardboard (7.80%), metals/aluminium (2.20%) and tetrabrik (2.60%). The quality of MSW was not uniform between cities, with Garupá (61.50%) being the city that generated the most organic waste, followed by Posadas (57.50%) and ending with Candelaria (29.00%) with a marked decrease. As a limitation of the method, it was determined that the results are representative of each city and of the winter season.","PeriodicalId":21243,"journal":{"name":"Revista de Ciencia y Tecnología","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79278244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.36995/j.recyt.2022.38.006
D. A. Vigo, N. R. Siviero, C. Schvezov
Plinthite (Gr. Plinthos, brick) is a residual soil that occupies 60 million hectares on the planet. The Province of Misiones (Argentina) is the border zone of South America where this soil appears, locally called in its cemented state or petroplinthite as itácurú (stone toad in the Guarani language). Four centuries ago, it was used in the form of blocks cut from nearby deposits for the construction of the Jesuit reductions since 1601 in Loreto. Since then, there is a time jump until it is used in the construction of Coastal Route No. 2. In view of its potential and in the absence of systematic studies, this report aims to promote this mining resource for the restoration of reductions, constructions allegorical and road use. To this end, prospecting has been carried out in search of new deposits and others previously exploited that have been detected and evaluated; some used by the Jesuits. The location, size and approximate power of each of the explored deposits are presented, as well as the geotechnical values. The experimental results of the physical-mechanical properties and the effects on the properties of the different drying/wetting cycles carried out are presented and analyzed.
Plinthite (Gr. Plinthos，砖)是地球上占地6000万公顷的残余土壤。米西奥内斯省(阿根廷)是南美洲的边境地区，这种土壤出现在那里，当地称其为胶结状态或petroplinthite为itácurú(瓜拉尼语中的石蟾蜍)。四个世纪前，它被用作从附近沉积物中切割出来的石块，用于建造自1601年以来在洛雷托的耶稣会会堂。从那时起，有一个时间跳跃，直到它被用于建设沿海路线2号。鉴于其潜力，在缺乏系统研究的情况下，本报告旨在促进这种采矿资源的恢复、建筑和道路使用。为此目的，进行了勘探工作，寻找新的矿藏和以前开采过的、已被发现和评价过的其他矿藏;有些是耶稣会使用的。介绍了每个已勘探矿床的位置、大小和近似功率，以及岩土工程值。给出并分析了不同干湿循环对其物理力学性能的影响及实验结果。
{"title":"Location and characterization of the Misiones plinthite (Argentina)","authors":"D. A. Vigo, N. R. Siviero, C. Schvezov","doi":"10.36995/j.recyt.2022.38.006","DOIUrl":"https://doi.org/10.36995/j.recyt.2022.38.006","url":null,"abstract":"Plinthite (Gr. Plinthos, brick) is a residual soil that occupies 60 million hectares on the planet. The Province of Misiones (Argentina) is the border zone of South America where this soil appears, locally called in its cemented state or petroplinthite as itácurú (stone toad in the Guarani language). Four centuries ago, it was used in the form of blocks cut from nearby deposits for the construction of the Jesuit reductions since 1601 in Loreto. Since then, there is a time jump until it is used in the construction of Coastal Route No. 2. In view of its potential and in the absence of systematic studies, this report aims to promote this mining resource for the restoration of reductions, constructions allegorical and road use. To this end, prospecting has been carried out in search of new deposits and others previously exploited that have been detected and evaluated; some used by the Jesuits. The location, size and approximate power of each of the explored deposits are presented, as well as the geotechnical values.\u0000The experimental results of the physical-mechanical properties and the effects on the properties of the different drying/wetting cycles carried out are presented and analyzed.","PeriodicalId":21243,"journal":{"name":"Revista de Ciencia y Tecnología","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82667412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.36995/j.recyt.2022.38.002
Alejandro A. Vistorte Salgado, F. E. Hernández Montero, Gianna Arencibia Castellanos
In order to automate the determination of gait parameters, a system capable of acquiring data from inertial units, exploiting their maximum sampling frequency, was developed. The study of gait is one of the fundamental indicators for the evaluation of physical performance. It allows the estimation of the functional deterioration of the elderly in an objective way, so several tests have been designed to evaluate it. The system developed has two fundamental elements: an electronic device and a desktop application. The electronic device has the function of collecting data from the MPU-9255 sensor using an ESP32 to set the sampling rate, transmitting the data via WiFi to the computer and monitoring the system’s battery. The desktop application allows the electronic device to be configured and controlled, as well as receiving, displaying and storing the data. As a result, a prototype capable of operating at a sampling frequency of 1 kHz was built. Tests carried out on the system demonstrate its reliability and allow the limits of sampling frequency and working distance to be set.
{"title":"Electronic device for gait analysis","authors":"Alejandro A. Vistorte Salgado, F. E. Hernández Montero, Gianna Arencibia Castellanos","doi":"10.36995/j.recyt.2022.38.002","DOIUrl":"https://doi.org/10.36995/j.recyt.2022.38.002","url":null,"abstract":"In order to automate the determination of gait parameters, a system capable of acquiring data from inertial units, exploiting their maximum sampling frequency, was developed. The study of gait is one of the fundamental indicators for the evaluation of physical performance. It allows the estimation of the functional deterioration of the elderly in an objective way, so several tests have been designed to evaluate it. The system developed has two fundamental elements: an electronic device and a desktop application. The electronic device has the function of collecting data from the MPU-9255 sensor using an ESP32 to set the sampling rate, transmitting the data via WiFi to the computer and monitoring the system’s battery. The desktop application allows the electronic device to be configured and controlled, as well as receiving, displaying and storing the data. As a result, a prototype capable of operating at a sampling frequency of 1 kHz was built. Tests carried out on the system demonstrate its reliability and allow the limits of sampling frequency and working distance to be set.","PeriodicalId":21243,"journal":{"name":"Revista de Ciencia y Tecnología","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75289792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.36995/j.recyt.2022.38.009
S. Grenón, L. Leguizamón, A. del Valle, S. Pallarès, M. S. Salvi Grabulosa, M. V. von Specht
Our objective was to evaluate the presence and microbiological characteristics of Shigella in children with acute diarrhoeal disease (ADD), attended at the Paediatric Hospital of Posadas, Misiones between 2016 and 2019. All ADEs documented in the laboratory were included. Standardised microbiological techniques were used. We considered age groups: infants (L)≤ 2 years, preschool (PE): 3-5 years, school (E): 5- 14 years. We recorded 106/2128 (5%) Shigella spp. isolates, with a higher frequency in summer (54.5%). S. flexneri (64; 60.4% serotypes 02(54), 03(3), AA479(2) and 01(1)) and S. sonnei (37; 34.9%) predominated. Mean age: 4.2 years (1.2 m-11a). Those < 5 years old were the most affected (70 %). Hospitalisation was required in 7.7%. S. flexneri/S. sonnei were: in L (42.9% vs 27.7%) and in E (27 vs 42.9%). Overall resistance to AMP was 72.7% and 58.1% to TMS. 97% were sensitive to furazolidone (FUR), cefpodoxime and fluorinated quinolones. In S. flexneri the % resistance to AMP/TMS was (78.7/44.8) and in S. sonnei (61.8/80.6) p< 0.05). We detected 6 resistance phenotypes with low multi-resistance (2.6%). FUR remains an empirical therapeutic option in outpatients.
我们的目标是评估2016年至2019年在米西奥内斯波萨达斯儿科医院就诊的急性腹泻病(ADD)儿童中志贺氏菌的存在和微生物学特征。所有在实验室记录的ade都包括在内。采用标准化微生物学技术。我们考虑了年龄组:婴儿(L)≤2岁，学龄前儿童(PE): 3-5岁，学校(E): 5- 14岁。志贺氏菌分离株106/2128株(5%)，夏季发生率较高(54.5%)。flexneri (64;60.4%血清型02(54)、03(3)、AA479(2)、01(1)和sonnei沙门氏菌(37);34.9%)成为主流。平均年龄:4.2岁(1.2 m-11a)。5岁以下儿童受影响最大(70%)。需要住院治疗的占7.7%。美国flexneri / S。分别为:L组(42.9% vs 27.7%)和E组(27% vs 42.9%)。对AMP和TMS的总体耐药率分别为72.7%和58.1%。97%对呋喃唑酮、头孢多肟和氟喹诺酮类药物敏感。flexneri和sonnei对AMP/TMS的耐药率分别为(78.7/44.8)和(61.8/80.6)，p< 0.05。我们检测到6种低多重耐药表型(2.6%)。FUR仍然是门诊患者的经验性治疗选择。
{"title":"Prevalence and antimicrobial profile of Shigella causing acute diarrheal disease in pediatrics in Posadas, Misiones","authors":"S. Grenón, L. Leguizamón, A. del Valle, S. Pallarès, M. S. Salvi Grabulosa, M. V. von Specht","doi":"10.36995/j.recyt.2022.38.009","DOIUrl":"https://doi.org/10.36995/j.recyt.2022.38.009","url":null,"abstract":"Our objective was to evaluate the presence and microbiological characteristics of Shigella in children with acute diarrhoeal disease (ADD), attended at the Paediatric Hospital of Posadas, Misiones between 2016 and 2019. All ADEs documented in the laboratory were included. Standardised microbiological techniques were used. We considered age groups: infants (L)≤ 2 years, preschool (PE): 3-5 years, school (E): 5- 14 years. We recorded 106/2128 (5%) Shigella spp. isolates, with a higher frequency in summer (54.5%). S. flexneri (64; 60.4% serotypes 02(54), 03(3), AA479(2) and 01(1)) and S. sonnei (37; 34.9%) predominated. Mean age: 4.2 years (1.2 m-11a). Those < 5 years old were the most affected (70 %). Hospitalisation was required in 7.7%. S. flexneri/S. sonnei were: in L (42.9% vs 27.7%) and in E (27 vs 42.9%). Overall resistance to AMP was 72.7% and 58.1% to TMS. 97% were sensitive to furazolidone (FUR), cefpodoxime and fluorinated quinolones. In S. flexneri the % resistance to AMP/TMS was (78.7/44.8) and in S. sonnei (61.8/80.6) p< 0.05). We detected 6 resistance phenotypes with low multi-resistance (2.6%). FUR remains an empirical therapeutic option in outpatients.","PeriodicalId":21243,"journal":{"name":"Revista de Ciencia y Tecnología","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84246169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.36995/j.recyt.2022.38.008
Matheus Pedron Cassol, Alexandre Rafael Lenz, Rudinei Zacaria, Scheila de Avila e Silva
The current paper seeks to approach, using a workflow, basics subjects of the bioinformatic field and also useful informations to consider during the development of in silico researches. Installation and general usage of multiple softwares related to different sections of the genome annotation process were also presented. At last, an model organism, Staphylococcus aureus, was sequenced in two different softwares, SPAdes and IDBA-UD, seeking further comparison and evaluation of the process as a whole. The quality evaluation of the assemble was established by tests on QUAST, BUSCO and Augustus, supported by BLASTP. Results: QUAST evaluation returned genome coverage values above 98% in both test cases, pointing towards a trustworthy assemble for this organism. Via SPAdes were needed less computational resources, but, using IDBA-UD the sequences found were more contiguous. Results deriving from BUSCO showed only one expected gene difference. Some proteins and genes predicted by Augustus led to hits, sequences already studied in that organism, using the BLASTP program.
{"title":"Computational Environment for Genomic Sequencing and Annotation: a workflow for application in projects by life researchers","authors":"Matheus Pedron Cassol, Alexandre Rafael Lenz, Rudinei Zacaria, Scheila de Avila e Silva","doi":"10.36995/j.recyt.2022.38.008","DOIUrl":"https://doi.org/10.36995/j.recyt.2022.38.008","url":null,"abstract":"The current paper seeks to approach, using a workflow, basics subjects of the bioinformatic field and also useful informations to consider during the development of in silico researches. Installation and general usage of multiple softwares related to different sections of the genome annotation process were also presented. At last, an model organism, Staphylococcus aureus, was sequenced in two different softwares, SPAdes and IDBA-UD, seeking further comparison and evaluation of the process as a whole. The quality evaluation of the assemble was established by tests on QUAST, BUSCO and Augustus, supported by BLASTP. Results: QUAST evaluation returned genome coverage values above 98% in both test cases, pointing towards a trustworthy assemble for this organism. Via SPAdes were needed less computational resources, but, using IDBA-UD the sequences found were more contiguous. Results deriving from BUSCO showed only one expected gene difference. Some proteins and genes predicted by Augustus led to hits, sequences already studied in that organism, using the BLASTP program.","PeriodicalId":21243,"journal":{"name":"Revista de Ciencia y Tecnología","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88184486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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