Y. López-Ramírez, E. Cabañas-García, C. Areche, G. Trejo-Tapia, E. Pérez-Molphe-Balch, Y. A. Gómez-Aguirre
It has been demonstrated that some species of the Yucca genus are a source of metabolites with functional properties, as is Yucca carnerosana (Trel.) McKelvey with antifungal activity. This research aimed to induce the formation of callus tissue in Y. carnerosana , to know the growth kinetic, and to analyze the metabolite profile of the formed tissue and plants propagated under in vitro and ex vitro conditions. Callus induction was achieved using Murashige & Skoog (MS) medium supplemented with 4.4 µ M benzyladenine and 4.1 µ M 4-aminotrichloropicolinic acid (Picloram). The growth kinetics of callus tissue was characterized by a latency phase achieved at the second week of culture, followed by an exponential growth until the fourth week. The culture showed a specific growth rate of 0.0258 d − 1 ; the doubling time was 26.866 days, and the growth index was 5.9091. The metabolite profile was analyzed using Ultra-High-Performance Liquid Chromatography coupled to Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS / MS). The chromatographic and mass spectral analysis allowed the separation and identification of 22 compounds in callus tissue, 26 in in vitro plants, and 27 in ex vitro plants. Our results indicate that the callus tissue and the in vitro and ex vitro plants of Y. carnerosana may be a source of metabolites of interest.
{"title":"Callus induction and phytochemical profiling of Yucca carnerosana (Trel.) McKelvey obtained from in vitro cultures","authors":"Y. López-Ramírez, E. Cabañas-García, C. Areche, G. Trejo-Tapia, E. Pérez-Molphe-Balch, Y. A. Gómez-Aguirre","doi":"10.24275/RMIQ/BIO2209","DOIUrl":"https://doi.org/10.24275/RMIQ/BIO2209","url":null,"abstract":"It has been demonstrated that some species of the Yucca genus are a source of metabolites with functional properties, as is Yucca carnerosana (Trel.) McKelvey with antifungal activity. This research aimed to induce the formation of callus tissue in Y. carnerosana , to know the growth kinetic, and to analyze the metabolite profile of the formed tissue and plants propagated under in vitro and ex vitro conditions. Callus induction was achieved using Murashige & Skoog (MS) medium supplemented with 4.4 µ M benzyladenine and 4.1 µ M 4-aminotrichloropicolinic acid (Picloram). The growth kinetics of callus tissue was characterized by a latency phase achieved at the second week of culture, followed by an exponential growth until the fourth week. The culture showed a specific growth rate of 0.0258 d − 1 ; the doubling time was 26.866 days, and the growth index was 5.9091. The metabolite profile was analyzed using Ultra-High-Performance Liquid Chromatography coupled to Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS / MS). The chromatographic and mass spectral analysis allowed the separation and identification of 22 compounds in callus tissue, 26 in in vitro plants, and 27 in ex vitro plants. Our results indicate that the callus tissue and the in vitro and ex vitro plants of Y. carnerosana may be a source of metabolites of interest.","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"156 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77625998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Pessoa-e-Silva, L. Moura-Andrade, F. A. Silva-Mota, W. F. Alencar-Borges
{"title":"Multi alcohols continuous unit for biodiesel production: Design and automation","authors":"R. Pessoa-e-Silva, L. Moura-Andrade, F. A. Silva-Mota, W. F. Alencar-Borges","doi":"10.24275/rmiq/sim2141","DOIUrl":"https://doi.org/10.24275/rmiq/sim2141","url":null,"abstract":"","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84888087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. D. Herrera-Zuñiga, M. González-Palma, G. Díaz-Godínez, D. C. Martínez-Carrera, M. C. Sánchez-Hernández, R. Díaz-Godínez
Pleurotus ostreatus is a basidiomycete fungus capable of producing oxidases involved in the degradation of lignin, such as laccase (Lac), manganese peroxidase (MnP), versatile peroxidase (VP), veratryl alcohol oxidase (VAO) and dye-decolorizing peroxidase (DyP). In this research, the molecular docking showed that the interaction between Mn-ion, ABTS or DMP ligand with the respective oxidases studied were strongly supported by exposed GLU and ASP charged residues H-bonded or hydrophobic-bonded, in most of the complexes, mainly GLU and ASP played a very important role in the union, especially in the presence of the Mn-ion. On the other hand, the growth and activity of such enzymes of Pleurotus ostreatus ARS 3526 grown in both, submerged fermentation (SmF) and solid-state fermentation (SSF) were evaluated. The specific growth rate in SSF was 2.5 times higher than in SmF. The values of activity of Lac, VP and DyP were higher in the SSF, of the VAO activity was similar in both fermentation systems and SmF had the higher MnP activity value in comparison with SSF. This study provides evidence of the enzymatic potential of this fungus and shows the similarities in charged amino acids when used in their catalytic interactions, and the intimate relationship between the enzyme and its substrate.
{"title":"Molecular docking of oxidases from Pleurotus ostreatus and the activity of those produced by ARS 3526 strain grown in both, submerged and solid-state fermentations","authors":"L. D. Herrera-Zuñiga, M. González-Palma, G. Díaz-Godínez, D. C. Martínez-Carrera, M. C. Sánchez-Hernández, R. Díaz-Godínez","doi":"10.24275/rmiq/bio2076","DOIUrl":"https://doi.org/10.24275/rmiq/bio2076","url":null,"abstract":"Pleurotus ostreatus is a basidiomycete fungus capable of producing oxidases involved in the degradation of lignin, such as laccase (Lac), manganese peroxidase (MnP), versatile peroxidase (VP), veratryl alcohol oxidase (VAO) and dye-decolorizing peroxidase (DyP). In this research, the molecular docking showed that the interaction between Mn-ion, ABTS or DMP ligand with the respective oxidases studied were strongly supported by exposed GLU and ASP charged residues H-bonded or hydrophobic-bonded, in most of the complexes, mainly GLU and ASP played a very important role in the union, especially in the presence of the Mn-ion. On the other hand, the growth and activity of such enzymes of Pleurotus ostreatus ARS 3526 grown in both, submerged fermentation (SmF) and solid-state fermentation (SSF) were evaluated. The specific growth rate in SSF was 2.5 times higher than in SmF. The values of activity of Lac, VP and DyP were higher in the SSF, of the VAO activity was similar in both fermentation systems and SmF had the higher MnP activity value in comparison with SSF. This study provides evidence of the enzymatic potential of this fungus and shows the similarities in charged amino acids when used in their catalytic interactions, and the intimate relationship between the enzyme and its substrate.","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78254845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. N. Cárdenas-Medina, M.C. Fajardo-Ortiz, B. Schettino-Bermúdez, M. Meraz-Rodríguez, P. Castilla-Hernández
The cheese industry produces residues such as acid cheese whey. The latter has a high organic-matter concentration and other nutrients that are severe environmental contaminants. The aim of this study was to evaluate the acidogenesis / methanogenesis of acid cheese whey with short Hydraulic Retention Times (HRT) in Hybrid-UASB reactors, following the evolution of the organic matter, ammonium, and orthophosphate. The acidogenic reactor was operated at an HRT of 0.5 days, while for the methanogenic reactor, this ranged from 1-2 days; both reactors were operated in series. Results showed that the overall HRT of 1.5 days allowed optimal removal (89-96.8%) with organic loading rates of between 2.5 and 10.7 g COD / L-d, while at 14.2-24.9 g COD / L-d, best results were achieved at 2.5 days of overall HRT. Highest methane productivity ranged from 0.99-1.15 L CH 4 / L reactor -d (0.238-0.308 L CH 4 / g COD degraded ). Ammonium formation was only significant in methanogenesis (85 mg / L), while orthophosphates showed no change throughout the entire process. In conclusion, Hybrid-UASB reactors operated at short HRT were suitable for the acidogenesis / methanogenesis of this wastewater. Furthermore, methane can be utilized for energy generation, and ammonium and orthophosphate might be employed for the development of high value-added products. L CH 4 / g DQO degradado ). La formación de amonio únicamente fue significativa en metanogénesis (85 mg / L); los ortofosfatos no mostraron cambio a través de todo el proceso. En conclusión los reactores UASB-híbridos operados a cortos TRH fueron adecuados para la acidogénesis / metanogénesis del agua residual ensayada. Además, el metano puede ser usado para la generación de energía; el amonio y ortofosfatos podrían emplearse para el desarrollo de productos de alto valor agregado. Palabras clave : Lactosuero ácido, acidogénesis / metanogénesis, reactor UASB-híbrido, efluentes industriales, nutrientes.
奶酪工业产生诸如酸性奶酪乳清之类的残留物。后者的有机物和其他营养物质浓度高，是严重的环境污染物。本研究的目的是评估在Hybrid-UASB反应器中具有短水力停留时间(HRT)的酸性奶酪乳清的产酸/产甲烷，跟踪有机物、铵和正磷酸盐的演变。产酸反应器的HRT运行时间为0.5天，而产甲烷反应器的HRT运行时间为1-2天;两个反应堆是串联运行的。结果表明，总HRT为1.5 d时，有机负荷率为2.5 ~ 10.7 g / L-d，去除率为89 ~ 96.8%;总HRT为2.5 d时，COD负荷率为14.2 ~ 24.9 g / L-d，去除率最高。最高甲烷产率为0.99 ~ 1.15 L ch4 / L反应器d (0.238 ~ 0.308 L ch4 / g COD降解)。铵的形成仅在产甲烷(85 mg / L)时显著，而正磷酸盐在整个过程中没有变化。综上所述，在短HRT下运行的混合式uasb反应器适合该废水的产酸/产甲烷。此外，甲烷可用于发电，铵和正磷酸盐可用于开发高附加值产品。L ch4 / g DQO降解物)。La formación de amonio únicamente fue significant en metanogacnesis (85 mg / L);在此过程中，不存在最严重的环境污染问题。En conclusión los reactor UASB-híbridos operados a cortos TRH fueros adados para acidogsamis / metanogsamis del aguersayada。Además, el metano puede ser usado para generación de energía;El amonio y ortofofatos podrían empleas para El desrollo de productos de alto valor aggregado。Palabras clave:乳酸菌ácido，酸酐/酸酐，反应器UASB-híbrido，工业污水，营养品。
{"title":"Acidogenesis/methanogenesis from acid cheese whey in hybrid-UASB reactors","authors":"K. N. Cárdenas-Medina, M.C. Fajardo-Ortiz, B. Schettino-Bermúdez, M. Meraz-Rodríguez, P. Castilla-Hernández","doi":"10.24275/rmiq/ia1420","DOIUrl":"https://doi.org/10.24275/rmiq/ia1420","url":null,"abstract":"The cheese industry produces residues such as acid cheese whey. The latter has a high organic-matter concentration and other nutrients that are severe environmental contaminants. The aim of this study was to evaluate the acidogenesis / methanogenesis of acid cheese whey with short Hydraulic Retention Times (HRT) in Hybrid-UASB reactors, following the evolution of the organic matter, ammonium, and orthophosphate. The acidogenic reactor was operated at an HRT of 0.5 days, while for the methanogenic reactor, this ranged from 1-2 days; both reactors were operated in series. Results showed that the overall HRT of 1.5 days allowed optimal removal (89-96.8%) with organic loading rates of between 2.5 and 10.7 g COD / L-d, while at 14.2-24.9 g COD / L-d, best results were achieved at 2.5 days of overall HRT. Highest methane productivity ranged from 0.99-1.15 L CH 4 / L reactor -d (0.238-0.308 L CH 4 / g COD degraded ). Ammonium formation was only significant in methanogenesis (85 mg / L), while orthophosphates showed no change throughout the entire process. In conclusion, Hybrid-UASB reactors operated at short HRT were suitable for the acidogenesis / methanogenesis of this wastewater. Furthermore, methane can be utilized for energy generation, and ammonium and orthophosphate might be employed for the development of high value-added products. L CH 4 / g DQO degradado ). La formación de amonio únicamente fue significativa en metanogénesis (85 mg / L); los ortofosfatos no mostraron cambio a través de todo el proceso. En conclusión los reactores UASB-híbridos operados a cortos TRH fueron adecuados para la acidogénesis / metanogénesis del agua residual ensayada. Además, el metano puede ser usado para la generación de energía; el amonio y ortofosfatos podrían emplearse para el desarrollo de productos de alto valor agregado. Palabras clave : Lactosuero ácido, acidogénesis / metanogénesis, reactor UASB-híbrido, efluentes industriales, nutrientes.","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88805863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. D. Castilla-Marroquín, R. Hernández-Martínez, H. D. L. Vequia, M. Ríos-Corripio, J. R. Hernández-Rosas, M. Rojas-López, F. Hernández-Rosas, Colegio de Postgraduados
The sugarcane agri-food industry boosts the Mexican economy in producing regions. Basing its relevance in sugar production yields. However, by-products are not widely exploited leaving an opportunity for diversification. In this study, three microorganism isolates (A, B, and C) were obtained from sugarcane kefir; the morphology of isolates B and C corresponded to the lactic acid bacterial genus Leuconostoc . Thus, we examined the potential for these isolates to produce EPSs, like dextran, a molecule with applications in pharmaceuticals, industrials, and foods. The experiment was performed adjusting the active culture concentration to 1 × 10 6 colony-forming units (CFU) / ml, the culture was maintained at 37°C in agitation at 150 rpm. The obtained EPSs were purified by ethanol and cold acetone precipitation. The results showed that B and C bacterial isolates had the capacity to produce EPSs (14 g / L for isolate B and 32 g / L for isolate C) after 24 h. Fourier-transform infrared spectroscopy (FT-IR) characterization indicated that the EPS was dextran. Further, the produced biopolymer had high solubility in water, avoided freezing at -4°C, and boiled at 85°C.
甘蔗农业食品产业促进了墨西哥生产地区的经济。根据其与糖产量的相关性。然而，副产品并未得到广泛利用，这为多样化留下了机会。本研究从甘蔗开菲尔中分离得到三种微生物(A、B和C);分离株B和C的形态与乳酸菌Leuconostoc属一致。因此，我们研究了这些分离株产生eps的潜力，如右旋糖酐，一种在制药、工业和食品中应用的分子。调节活性培养浓度为1 × 10 6菌落形成单位(CFU) / ml, 37℃搅拌，转速150 rpm。所得eps用乙醇和冷丙酮沉淀法纯化。结果表明，菌株B和菌株C在24 h后均能产生EPS(菌株B为14 g / L，菌株C为32 g / L)，傅里叶变换红外光谱(FT-IR)表征表明EPS为葡聚糖。此外，所制备的生物聚合物在水中具有高溶解度，避免在-4°C下冻结，并在85°C下煮沸。
{"title":"Dextran synthesis by native sugarcane microorganisms","authors":"J. D. Castilla-Marroquín, R. Hernández-Martínez, H. D. L. Vequia, M. Ríos-Corripio, J. R. Hernández-Rosas, M. Rojas-López, F. Hernández-Rosas, Colegio de Postgraduados","doi":"10.24275/rmiq/bio1793","DOIUrl":"https://doi.org/10.24275/rmiq/bio1793","url":null,"abstract":"The sugarcane agri-food industry boosts the Mexican economy in producing regions. Basing its relevance in sugar production yields. However, by-products are not widely exploited leaving an opportunity for diversification. In this study, three microorganism isolates (A, B, and C) were obtained from sugarcane kefir; the morphology of isolates B and C corresponded to the lactic acid bacterial genus Leuconostoc . Thus, we examined the potential for these isolates to produce EPSs, like dextran, a molecule with applications in pharmaceuticals, industrials, and foods. The experiment was performed adjusting the active culture concentration to 1 × 10 6 colony-forming units (CFU) / ml, the culture was maintained at 37°C in agitation at 150 rpm. The obtained EPSs were purified by ethanol and cold acetone precipitation. The results showed that B and C bacterial isolates had the capacity to produce EPSs (14 g / L for isolate B and 32 g / L for isolate C) after 24 h. Fourier-transform infrared spectroscopy (FT-IR) characterization indicated that the EPS was dextran. Further, the produced biopolymer had high solubility in water, avoided freezing at -4°C, and boiled at 85°C.","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77951896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Divanery Rodriguez-Gomez, L. Garate-Velez, A. L. Morales-Piña, M. Gutiérrez-Rojas, N. Velasco-Alvarez
The e ff ects of an electric field on conidia production by Metarhizium anisopliae in solid state culture were determined. A 450-mL electrochemical cell (EC) with titanium ruthenium-oxide coated electrodes and packed with a mixture of rice: corn stubble, was inoculated with 1 × 10 6 conidia (g of initial dry support) − 1 and incubated for 8 days (30 ºC). Four days after starting the culture, a current density of 0.09 mA cm − 2 was applied for 24 h. The moisture kept constant (75%), with and without electric field. With electric field, conidiation (4.9 × 10 8 ± 3 . 7 × 10 7 conidia (g of initial dry support) − 1 ) did not show statistically significant di ff erences, but their viability and germination (67 and 45%, respectively) were lower than in the non-perturbated conidia. Total mortality of Tenebrio molitor larvae provoked by conidia produced in presence of the electric field was 40% higher compared with the control. The study showed that the application of electric field improving the conidial infectivity. This new approach is susceptible to be optimized to other fungi for biological control with the purpose of improving field performance and further investigations on the modification of cellular mechanisms by the electric field stimuli.
{"title":"Increase in the mortality of the entomopathogenic fungus Metarhizium anisopliae due to the application of an electric field during conidiation","authors":"Divanery Rodriguez-Gomez, L. Garate-Velez, A. L. Morales-Piña, M. Gutiérrez-Rojas, N. Velasco-Alvarez","doi":"10.24275/rmiq/bio1601","DOIUrl":"https://doi.org/10.24275/rmiq/bio1601","url":null,"abstract":"The e ff ects of an electric field on conidia production by Metarhizium anisopliae in solid state culture were determined. A 450-mL electrochemical cell (EC) with titanium ruthenium-oxide coated electrodes and packed with a mixture of rice: corn stubble, was inoculated with 1 × 10 6 conidia (g of initial dry support) − 1 and incubated for 8 days (30 ºC). Four days after starting the culture, a current density of 0.09 mA cm − 2 was applied for 24 h. The moisture kept constant (75%), with and without electric field. With electric field, conidiation (4.9 × 10 8 ± 3 . 7 × 10 7 conidia (g of initial dry support) − 1 ) did not show statistically significant di ff erences, but their viability and germination (67 and 45%, respectively) were lower than in the non-perturbated conidia. Total mortality of Tenebrio molitor larvae provoked by conidia produced in presence of the electric field was 40% higher compared with the control. The study showed that the application of electric field improving the conidial infectivity. This new approach is susceptible to be optimized to other fungi for biological control with the purpose of improving field performance and further investigations on the modification of cellular mechanisms by the electric field stimuli.","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78367204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Robles-Zamora, Daniela Enriquez-Ochoa, M. Ureña-Herrera, J. M. Aguilar-Yañez, Marion E. Brunck, Karla Mayolo-Deloisa
Major Royal Jelly Protein 1 (MRJP1) is the main protein component of the bee-produced complex mixture royal jelly, which is the only nutrient source for queen bees promoting increased lifespan, body size and fertility. Recombinant production of MRJP1 represents an alternative to direct extraction from royal jelly. Production in Pichia pastoris results in high density biomass, with a supernatant containing high amount of impurities. Various methods have been applied to recover and/or purify MRJP1. Here, exploiting the physicochemical properties of MRJP1, reverse phase chromatography (RPC), size exclusion chromatography (SEC) and ion-exchange chromatography (IEX) were investigated as alternative methods to recover MRJP1 directly from supernatant. All techniques showed a 57-kDa band in SDS-PAGE analysis, corresponding to the size of recombinant MRJP1, with contaminants attributed to culture media. However, SEC coupled to IEX evidenced a single peak in the chromatogram corresponding to MRJP1 which suggest it may be a good protocol to recover recombinant MRJP1 from P. pastoris supernatant. This approach serves as a procedure to identify MRJP1 in fermentation culture of P. pastoris. This is the first report about characterization of IEX-based recovery of recombinant Apis mellifera MRJP1 produced in Pichia pastoris without the use of histidine tags.
{"title":"Partial recovery of MRJP1 protein expressed in Pichia pastoris using chromatographic techniques","authors":"A. Robles-Zamora, Daniela Enriquez-Ochoa, M. Ureña-Herrera, J. M. Aguilar-Yañez, Marion E. Brunck, Karla Mayolo-Deloisa","doi":"10.24275/rmiq/bio1713","DOIUrl":"https://doi.org/10.24275/rmiq/bio1713","url":null,"abstract":"Major Royal Jelly Protein 1 (MRJP1) is the main protein component of the bee-produced complex mixture royal jelly, which is the only nutrient source for queen bees promoting increased lifespan, body size and fertility. Recombinant production of MRJP1 represents an alternative to direct extraction from royal jelly. Production in Pichia pastoris results in high density biomass, with a supernatant containing high amount of impurities. Various methods have been applied to recover and/or purify MRJP1. Here, exploiting the physicochemical properties of MRJP1, reverse phase chromatography (RPC), size exclusion chromatography (SEC) and ion-exchange chromatography (IEX) were investigated as alternative methods to recover MRJP1 directly from supernatant. All techniques showed a 57-kDa band in SDS-PAGE analysis, corresponding to the size of recombinant MRJP1, with contaminants attributed to culture media. However, SEC coupled to IEX evidenced a single peak in the chromatogram corresponding to MRJP1 which suggest it may be a good protocol to recover recombinant MRJP1 from P. pastoris supernatant. This approach serves as a procedure to identify MRJP1 in fermentation culture of P. pastoris. This is the first report about characterization of IEX-based recovery of recombinant Apis mellifera MRJP1 produced in Pichia pastoris without the use of histidine tags.","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78223318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nota aclaratoria respecto al licenciamiento de documentos publicados en Ingeniería: Revista de la Universidad de Costa Rica
关于发表在《工程:哥斯达黎加大学杂志》上的文件许可的说明
{"title":"Fe de errata","authors":"Marcela Adriana Quirós Garita","doi":"10.15517/ri.v30i2.43123","DOIUrl":"https://doi.org/10.15517/ri.v30i2.43123","url":null,"abstract":"Nota aclaratoria respecto al licenciamiento de documentos publicados en Ingeniería: Revista de la Universidad de Costa Rica","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74212808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. C. Briceño, N. Suarez, Camila Castro, C. Agudelo, L. González, S. Isaza
La llegada de la pandemia al pais trajo consigo la necesidad de ampliar la oferta de elementos de proteccion personal. Esto activo el desarrollo de nuevos dispositivos y materiales para cubrir los ...
由于这一流行病在该国的到来，需要扩大个人防护用品的供应。这促进了新设备和材料的开发，以覆盖……
{"title":"El boom de los tapabocas","authors":"J. C. Briceño, N. Suarez, Camila Castro, C. Agudelo, L. González, S. Isaza","doi":"10.16924/REVINGE.50.7","DOIUrl":"https://doi.org/10.16924/REVINGE.50.7","url":null,"abstract":"La llegada de la pandemia al pais trajo consigo la necesidad de ampliar la oferta de elementos de proteccion personal. Esto activo el desarrollo de nuevos dispositivos y materiales para cubrir los ...","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84350448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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