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Evaluation of irrigation requirement for the design of an irrigation system using a probabilistic approach for the estimation of evapotranspiration and rainfall 利用估算蒸发蒸腾和降雨量的概率方法评估灌溉系统设计的灌溉需求
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-10 DOI: 10.5935/1806-6690.20230048
ABST RACT - Reference evapotranspiration (ET 0 ) and rainfall are basic variables for estimating the net irrigation depth (NID). The objective of this study was to estimate the NID for designing irrigation systems in Piracicaba, SP, Brazil, using ET 0 and rainfall probability distributions. A 30-year ET 0 and rainfall dataset (1990–2019) was obtained from the ESALQ/USP weather station. The water balance between ET 0 and rainfall indicated July, August, and September as months of higher water de fi cit. Based on the fi rst-order Markov chain, August presented the highest water de fi cit. Rainfall and ET 0 were estimated on 19 probability levels, and four probability distributions such as normal, log-normal, beta, and mixed gamma were evaluated. The analysis of historical August series using accumulated values in periods of fi ve, ten, or 15 days is recommended for sizing irrigation designs in Piracicaba, SP, Brazil. The log-normal and mixed gamma probability distributions presented the best fi t for ET 0 and rainfall data, respectively. To reach a crop coe ffi cient K c = 1 in Piracicaba, SP, Brazil in August, the irrigation system should be designed for an NID of 4.1 mm day − 1 . The use of mean monthly rainfall and ET 0 values for designing irrigation systems underestimates the NID by a mean of 26.6% compared to estimates made at a probability of 75% at fi ve-, ten, and 15-day intervals because the mean rainfall values occurred with exceedance probabilities of < 36%, and mean ET 0 values occurred with non-exceedance
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引用次数: 0
Machinery traffi c and cover crop eff ects on water infi ltration rate in a Xanthic Hapludox 机械交通和覆盖作物对黄杨灌丛水分入渗速率的影响
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-10 DOI: 10.5935/1806-6690.20230049
- The e ff ects of machinery tra ffi c and cover crops on soil physical properties have been underexplored under no-till agricultural production in subtropical environments. The objective of this study was to quantify the soil water in fi ltration rate and related soil physical properties in response to tractor tra ffi c levels (0, 2, 5, and 20 passes) and the presence or absence of cover crops in a Xanthic Hapludox soil over two growing seasons under no-till conditions. The experiment was carried out in a randomized block design arranged in a factorial scheme with four replications. The tra ffi c factor was constituted by the number of passes with a 6 Mg weight tractor. The winter crop management practices factor consisted of the cover crops (i) black oat ( Avena strigosa ) in 2017 and turnip ( Raphanus sativus var. oleiferus ) in 2018 compared with (ii) fallow with spontaneous vegetation. The soil water in fi ltration rate increased with cover crops when compared to fallow. Regarding tractor tra ffi c levels, shortly after soil compaction, there was a lower water in fi ltration rate at 20 passes, 45 mm h -1 lower than the absence of tractor tra ffi c. The soil water in fi ltration rate was positively correlated with macroporosity and negatively correlated with soil bulk density. After 14 months of soil compaction caused by tractor tra ffi c, an improvement in the soil physical properties and the water in fi ltration rate was found, which were enhanced by the inclusion of cover crops.
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引用次数: 0
Physiological quality in seeds of the common bean is aff ected by the period of nutri-priming 普通豆种子的生理品质受营养化时间的影响
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-07-10 DOI: 10.5935/1806-6690.20230050
- Nutri-priming is a low-cost and highly e ff ective way of supplying seeds with micronutrients. The aim of this study was to evaluate the physiological quality of seeds of the Pérola cultivar of the common bean under nutrient solutions containing molybdenum, cobalt and zinc for di ff erent imbibition times. The experiment was carried out in the laboratory and greenhouse of the State University of Goiás UEG – UnU Ipameri, using a completely randomised experimental design, with eight micronutrient treatments (T1. Control; T2. Mo; T3. Zn; T4. Co; T5. Mo + Co; T6. Mo + Zn; T7. Zn + Co; T8. Mo + Co + Zn) and six imbibition times (6, 8, 12, 16, 20 and 24 hours). The variables under analysis were seed imbibition, germination test, fi rst germination count, accelerated ageing, seedling emergence, emergence speed index, shoot length, root length, total length, fresh weight, dry weight, and shoot and root biomass. The micronutrients a ff orded an increase in germination in the seed batches under evaluation; on the other hand, imbibition times of greater than four hours reduced the germination capacity of the seeds. It can be concluded that nutri-priming in the common bean is only bene fi cial for an imbibition time of up to four hours.
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引用次数: 0
Number of trials to estimate the condition number in rye traits 估计黑麦性状条件数的试验数
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-06-30 DOI: 10.5935/1806-6690.20230046
I. Neu, A. Cargnelutti Filho, D. L. Silveira, R. Pezzini, C. T. Bandeira
- Multicollinearity must be diagnosed in multivariate analyses. Among the indicators, the condition number can be used to quantify the degree of multicollinearity. Hence, this study sought to determine the number of measurements (trials) necessary to estimate the number of condition in linear correlation matrices between rye traits. Five uniformity trials were carried out with ‘BRS Progresso’ rye, and eight morphological traits and eight productive traits were evaluated, forming two groups. In each group of traits, six cases (combinations of traits) were planned and the multicollinearity diagnosis was performed. Repeatability analyses were performed using the following methods: analysis of variance, principal component analysis, and structural analysis, and the number of measurements (trials) was determined for di ff erent levels of precision. A higher condition number of repeatability coe ffi cients was obtained by the principal component methods (based on correlation and variance and covariance matrices) and structural analysis based on the variance and covariance matrix. A greater number of measurements (trials) is necessary to estimate the number of conditions in productive traits compared to morphological ones. One trial is enough to e ffi ciently estimate the condition number with a minimum accuracy of 80% in morphological and productive traits of rye, whereas at least three trials are required for 95% accuracy.
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引用次数: 0
Occurrence and spatial distribution of native acai groves in high-production areas of the Amazon region 亚马逊地区巴西莓高产地区原生巴西莓林的发生与空间分布
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-06-30 DOI: 10.5935/1806-6690.20230039
Daniele Cristina de Brito Lima Soares, H. Lima, S. R. Araújo, Robson José Carrera Ramos, A. J. S. Rocha
- The fl oodplain forests of the Amazon estuary have undergone constant change over recent years, where management techniques, especially intensive management, have had an impact on the dynamics of the vegetation and land use. These changes can be monitored using satellite data. With this in mind, the aim of this study was to evaluate the dynamics of ground vegetation on the islands of Jarimbu, Mamangal, Itaboca, Mutirão and Buçu in the district of Igarapé-Miri, Pará, using images from the RapidEye and Planet satellites. The unsupervised ISODATA classi fi cation method was used, generating distinct classes of vegetation between each island. To evaluate the e ffi ciency of the classi fi cation, an average of 200 random points were used, with another 30 points relating to the type of usage for each class. The Kappa index and overall precision were also analysed, in addition to calculating errors of omission and commission. Monitoring on a seven-year time scale using high-resolution satellites, a more than 50% increase in the Exposed Soil class was seen for the islands of Jarimbu, Mutirão and Itaboca, the latter responsible for an increase of more than 50% in the Urban Area class. On each of the fi ve islands, the Alluvial class, representing the areas of açaí groves, has emerged over the last seven years, increasing in area at the expense of a reduction in the Arboreal class. In this respect, the confusion matrix showed a mean accuracy for the islands of ‘very good’, with a mean overall precision of 77.74%, and a mean Kappa index of 0.73, indicating strong agreement with the reference data and the classi fi cation
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引用次数: 0
Use of B-mode ultrasonography for sex determination and maturation monitoring in Prochilodus brevis (Steindachner 1875) b型超声在短原蛾性别鉴定和成熟监测中的应用(Steindachner 1875)
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-06-30 DOI: 10.5935/1806-6690.20230047
C. Salmito‐Vanderley, Pedro Emidio Leite Moraes Ferreira, M. B. Souza, Guilherme Melo Madeira, B. F. Brito, Lívia Correia Magalhães, Carlos Henrique Sousa de Melo, A. R. Montenegro
- The Brazilian bocachico is a rheophilic fi sh from the northeast region of Brazil that holds great economic and scienti fi c interest. The absence of evident sexual dimorphism and the invasive character of sexing methods and evaluation of gonadal maturation are obstacles to its arti fi cial reproduction. In this scenario, ultrasonography emerges as a non-invasive method for sexing. The objectives of this study were to establish an ultrasound sexing method for Brazilian bocachico and select equations to estimate the gonadosomatic index (GSI), gonad weight (GW), and gonad volume (GV) of live bocachico. A total of 32 bocachico fi sh (18 females and 14 males) were analyzed ultrasonographically and morphologically. The association of morphometric and ultrasonographic parameters was investigated using Pearson Correlations. The following prediction equations were selected using multiple regression analysis: GSI = 16.22 × transvarea; GW = 30.3 × transvarea; and GV = 0.001260036 × pixels. A model capable of predicting the sex of the animals based on the ultrasonographic parameters was obtained using partial least-squares discriminant analysis with principal component analysis. The significance level adopted was P < 0.05. In conclusion, ultrasonography is a useful tool to perform sexing and evaluate the gonads during the breeding season, contributing to artificial reproduction in captivity.
巴西波卡西科是一种来自巴西东北部地区的嗜流变鱼类,具有巨大的经济和科学价值。缺乏明显的性别二态性和侵入性的性别方法和性腺成熟的评估是其人工繁殖的障碍。在这种情况下,超声检查成为一种非侵入性的性别鉴定方法。本研究的目的是建立巴西波卡西科的超声性别鉴定方法,并选择方程来估计活波卡西科的性腺指数(GSI)、性腺重量(GW)和性腺体积(GV)。对32条波卡西科鱼(雌鱼18条,雄鱼14条)进行了超声和形态学分析。利用Pearson相关性分析形态学参数与超声参数之间的关系。采用多元回归分析选择预测方程:GSI = 16.22 ×横截面积;GW = 30.3 ×横截面积;GV = 0.001260036 ×像素。采用偏最小二乘判别分析和主成分分析相结合的方法,建立了基于超声参数预测动物性别的模型。采用的显著性水平为P < 0.05。综上所述,在繁殖季节,超声检查是一种有用的工具来进行性别鉴定和评估性腺,有助于人工繁殖。
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引用次数: 0
Detection of nematodes in soybean crop by drone 大豆作物线虫的无人机检测
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-06-30 DOI: 10.5935/1806-6690.20230038
Bruno Henrique Tondato Arantes, V. Moraes, Alaerson Maia Geraldine, T. M. Alves, Alice Maria Albert, Gabriel Jesus da Silva, Gustavo Castoldi
- Global consumption of oilseeds has been growing progressively in the last fi ve growing seasons, in which soybean represents 60% of this sector. Thus, in order to maintain a high production in the region of Rio Verde, State of Goiás, against the phytopathological problems, this study aimed to de fi ne the best spectral range for the detection of H. glycines and P. brachyurus by linear regressions in soybean at R3 stage, as well as the elaboration of mathematical models through multiple linear regressions. For this, soil and root were sampled in the experimental area, as well as a fl ight was performed with the Sentera sensor. Data were used for the elaboration of regressions and for the validation of 2 mathematical models. Signi fi cant values were observed in simple linear regression only for cysts, in the visible range, with a good R² value for the Green, Red and 568 nm bands, to nonviable cysts. When working with the stepwise statistics, better results are found for H. glycines , which now has an R²(aj) of 0.7430 and P. brachyurus is then detected. From the mathematical model obtained with the multiple linear regression for non-viable cysts with an R²(aj) of 0.7430, it is possible to detect the spatial distribution of nematodes across the soybean fi eld, in order to perform a localized management, optimizing the applications. Good results are also possible using the mathematical model obtained by simple linear regression.
-全球油籽消费量在过去5个生长季节一直在逐步增长,其中大豆占该部门的60%。因此,为了解决Goiás州里约热内卢Verde地区的植物病理学问题,本研究旨在通过线性回归确定R3期大豆中H. glycines和P. brachyurus检测的最佳光谱范围,并通过多元线性回归建立数学模型。为此,在实验区取样土壤和根系,并使用Sentera传感器进行飞行。数据用于阐述回归和验证2个数学模型。在简单的线性回归中,仅在可见范围内,对于不活的囊肿,在绿色、红色和568 nm波段具有良好的R²值。当使用逐步统计时,发现H. glycines的结果更好,现在的R²(aj)为0.7430,然后检测到P. brachyurus。通过对非活包囊进行多元线性回归,得到R²(aj) = 0.7430的数学模型,可以检测线虫在大豆田间的空间分布,从而进行本地化管理,优化应用。用简单线性回归得到的数学模型也可以得到很好的结果。
{"title":"Detection of nematodes in soybean crop by drone","authors":"Bruno Henrique Tondato Arantes, V. Moraes, Alaerson Maia Geraldine, T. M. Alves, Alice Maria Albert, Gabriel Jesus da Silva, Gustavo Castoldi","doi":"10.5935/1806-6690.20230038","DOIUrl":"https://doi.org/10.5935/1806-6690.20230038","url":null,"abstract":"- Global consumption of oilseeds has been growing progressively in the last fi ve growing seasons, in which soybean represents 60% of this sector. Thus, in order to maintain a high production in the region of Rio Verde, State of Goiás, against the phytopathological problems, this study aimed to de fi ne the best spectral range for the detection of H. glycines and P. brachyurus by linear regressions in soybean at R3 stage, as well as the elaboration of mathematical models through multiple linear regressions. For this, soil and root were sampled in the experimental area, as well as a fl ight was performed with the Sentera sensor. Data were used for the elaboration of regressions and for the validation of 2 mathematical models. Signi fi cant values were observed in simple linear regression only for cysts, in the visible range, with a good R² value for the Green, Red and 568 nm bands, to nonviable cysts. When working with the stepwise statistics, better results are found for H. glycines , which now has an R²(aj) of 0.7430 and P. brachyurus is then detected. From the mathematical model obtained with the multiple linear regression for non-viable cysts with an R²(aj) of 0.7430, it is possible to detect the spatial distribution of nematodes across the soybean fi eld, in order to perform a localized management, optimizing the applications. Good results are also possible using the mathematical model obtained by simple linear regression.","PeriodicalId":21359,"journal":{"name":"Revista Ciencia Agronomica","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71157879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Productivity in the peanut under salt stress in soil with a cover of plant mulch 覆盖土壤盐胁迫下花生产量的研究
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-06-30 DOI: 10.5935/1806-6690.20230043
H. C. Sousa, T. V. D. A. Viana, G. G. Sousa, B. M. Azevedo, C. Lessa, M. Freire, Geovana F. Goes, Bubabar Baldé
- Peanut cultivation in the northeast of Brazil has been badly a ff ected by salts accumulating in the irrigation water; in this respect, the use of plant mulch is one way of mitigating salt stress. The aim of this study was to evaluate the e ff ect of irrigation using brackish water under di ff erent mulching strategies on productivity and water use e ffi ciency in the peanut. The experiment was carried out under fi eld conditions in Redenção, in the state of Ceará, in a randomised block design of split-plots with fi ve replications, in which the plots corresponded to two levels of electrical conductivity of the irrigation water (0.8 and 4.0 dS m -1 ), and the subplots to six mulching strategies based on the phenology of the crop (MS1: mulch throughout the cycle; MS2: fl owering stage; MS3: appearance of the gynophore; MS4: pod formation; MS5: fi nal fl owering stage; MS6: no mulch). The following were determined at the end of the experimental cycle: commercial, non-commercial and total number of pods, number of grains per pod, pod length and diameter, productivity and water use e ffi ciency. Irrigation with lower-salinity water, together with mulching during the phenological stages of the peanut, a ff ords better productive performance and greater water use e ffi ciency. Salt stress reduced the length and diameter of the pods and had a negative e ff ect on productivity and water use e ffi ciency, both with and without the use of plant mulch during the phenological stages.
{"title":"Productivity in the peanut under salt stress in soil with a cover of plant mulch","authors":"H. C. Sousa, T. V. D. A. Viana, G. G. Sousa, B. M. Azevedo, C. Lessa, M. Freire, Geovana F. Goes, Bubabar Baldé","doi":"10.5935/1806-6690.20230043","DOIUrl":"https://doi.org/10.5935/1806-6690.20230043","url":null,"abstract":"- Peanut cultivation in the northeast of Brazil has been badly a ff ected by salts accumulating in the irrigation water; in this respect, the use of plant mulch is one way of mitigating salt stress. The aim of this study was to evaluate the e ff ect of irrigation using brackish water under di ff erent mulching strategies on productivity and water use e ffi ciency in the peanut. The experiment was carried out under fi eld conditions in Redenção, in the state of Ceará, in a randomised block design of split-plots with fi ve replications, in which the plots corresponded to two levels of electrical conductivity of the irrigation water (0.8 and 4.0 dS m -1 ), and the subplots to six mulching strategies based on the phenology of the crop (MS1: mulch throughout the cycle; MS2: fl owering stage; MS3: appearance of the gynophore; MS4: pod formation; MS5: fi nal fl owering stage; MS6: no mulch). The following were determined at the end of the experimental cycle: commercial, non-commercial and total number of pods, number of grains per pod, pod length and diameter, productivity and water use e ffi ciency. Irrigation with lower-salinity water, together with mulching during the phenological stages of the peanut, a ff ords better productive performance and greater water use e ffi ciency. Salt stress reduced the length and diameter of the pods and had a negative e ff ect on productivity and water use e ffi ciency, both with and without the use of plant mulch during the phenological stages.","PeriodicalId":21359,"journal":{"name":"Revista Ciencia Agronomica","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71158693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Heavy metal fractionation in Fluvic Entisols under saline conditions in Northeastern Brazil 巴西东北部盐碱化条件下河流底泥中的重金属分馏
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-06-30 DOI: 10.5935/1806-6690.20230041
M. E. O. Escobar, Esdras Rocha da Silva, T. S. Oliveira, F. F. F. Hernandez
- The long-term use of agrichemicals might add some heavy metals to soils and, there is little information about the dynamics of these elements in soils under saline conditions in semiarid regions. This study aimed to investigate the dynamics of heavy metals in the di ff erent fractions of Fluvic Entisols with saline conditions under long term of coconut cultivation in a semiarid region. Composite soil samples were collected in fi ve agricultural areas and three areas under native vegetation. Physical, chemical, and heavy metal analyses were carried out in soil (semi total and fractionated contents). Quality of irrigation water and drainage water was also evaluated. Analytical results were normalized and subjected to analysis of variance, and means were compared by Scott-Knott test (P < 0.05). Pearson correlation analysis was also used to assure a minimum correlation of variables justifying its use in the matrix of a factorial analysis. Semi-total contents of Cr, Ni, Pb and Zn (except Cu in some areas) were lower than previous local assessment and national guiding values, indicating that they do not cause harmful alterations in soil quality. Analyses of waters showed high levels of HCO 3 , indicating moderate restriction to use (due to the high levels of EC, SAR, Na and Cl). Fractionation of heavy metals revealed low risk of environmental pollution since most metal contents were bound to the oxidic, organic and residual fractions. The water used in irrigation contributes to the contents of heavy metals and to the salinity condition of the evaluated soils.
{"title":"Heavy metal fractionation in Fluvic Entisols under saline conditions in Northeastern Brazil","authors":"M. E. O. Escobar, Esdras Rocha da Silva, T. S. Oliveira, F. F. F. Hernandez","doi":"10.5935/1806-6690.20230041","DOIUrl":"https://doi.org/10.5935/1806-6690.20230041","url":null,"abstract":"- The long-term use of agrichemicals might add some heavy metals to soils and, there is little information about the dynamics of these elements in soils under saline conditions in semiarid regions. This study aimed to investigate the dynamics of heavy metals in the di ff erent fractions of Fluvic Entisols with saline conditions under long term of coconut cultivation in a semiarid region. Composite soil samples were collected in fi ve agricultural areas and three areas under native vegetation. Physical, chemical, and heavy metal analyses were carried out in soil (semi total and fractionated contents). Quality of irrigation water and drainage water was also evaluated. Analytical results were normalized and subjected to analysis of variance, and means were compared by Scott-Knott test (P < 0.05). Pearson correlation analysis was also used to assure a minimum correlation of variables justifying its use in the matrix of a factorial analysis. Semi-total contents of Cr, Ni, Pb and Zn (except Cu in some areas) were lower than previous local assessment and national guiding values, indicating that they do not cause harmful alterations in soil quality. Analyses of waters showed high levels of HCO 3 , indicating moderate restriction to use (due to the high levels of EC, SAR, Na and Cl). Fractionation of heavy metals revealed low risk of environmental pollution since most metal contents were bound to the oxidic, organic and residual fractions. The water used in irrigation contributes to the contents of heavy metals and to the salinity condition of the evaluated soils.","PeriodicalId":21359,"journal":{"name":"Revista Ciencia Agronomica","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71158136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cryopreservation of Genipa americana seeds 美洲属植物种子的低温保存
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-06-30 DOI: 10.5935/1806-6690.20230045
Rafaela Ribeiro de Souza, P. Paiva, R. Freitas, Diogo Pedrosa Corrêa da Silva, Michele Valquíria dos Reis, F. Nery, R. Paiva
- Genipa americana L. is a forest species of high socioeconomic potential. However, predatory extractivism actions threaten its existence, making it necessary to adopt conservationist practices. G. americana seeds show sensitivity to desiccation and cooling, making it unfeasible for conservation by conventional methods. Thus, cryopreservation is an promising alternative for the long-term conservation of species that produce unorthodox seeds, such as the genipap. In this sense, the objective was to cryopreservation of seeds of G. americana and to evaluate the effects of desiccation and freezing on germination and establishment of seedlings. Initially, seeds were dehydrated in silica gel 0, 16, 18, 20, 22 and 24 h, and then were cryopreserved in liquid nitrogen (LN) (-196 °C) during 24 h. After thawing, the viability and germination were analyzed. Dehydrated and non-cryopreserved seeds were also analyzed. The silica gel desiccation caused a reduction in viability and germination of the seeds of G. americana . The initial seed water content was so high (47%) that storage in LN (+LN) without prior dehydration treatment resulted in seed mortality. It was veri fi ed that, the dehydration in silica gel for the minimum time 20 h (corresponding to 14% water content) provides greater freezing tolerance, allowing the successful cryopreservation. Silica gel dehydration followed by immersion in LN was shown to be highly efficient for cryopreservation of seeds of G. americana , besides germinatio after thawing, high survival rates (100%) were obtained, with growth and normal establishment of the seedlings after acclimatization.
{"title":"Cryopreservation of Genipa americana seeds","authors":"Rafaela Ribeiro de Souza, P. Paiva, R. Freitas, Diogo Pedrosa Corrêa da Silva, Michele Valquíria dos Reis, F. Nery, R. Paiva","doi":"10.5935/1806-6690.20230045","DOIUrl":"https://doi.org/10.5935/1806-6690.20230045","url":null,"abstract":"- Genipa americana L. is a forest species of high socioeconomic potential. However, predatory extractivism actions threaten its existence, making it necessary to adopt conservationist practices. G. americana seeds show sensitivity to desiccation and cooling, making it unfeasible for conservation by conventional methods. Thus, cryopreservation is an promising alternative for the long-term conservation of species that produce unorthodox seeds, such as the genipap. In this sense, the objective was to cryopreservation of seeds of G. americana and to evaluate the effects of desiccation and freezing on germination and establishment of seedlings. Initially, seeds were dehydrated in silica gel 0, 16, 18, 20, 22 and 24 h, and then were cryopreserved in liquid nitrogen (LN) (-196 °C) during 24 h. After thawing, the viability and germination were analyzed. Dehydrated and non-cryopreserved seeds were also analyzed. The silica gel desiccation caused a reduction in viability and germination of the seeds of G. americana . The initial seed water content was so high (47%) that storage in LN (+LN) without prior dehydration treatment resulted in seed mortality. It was veri fi ed that, the dehydration in silica gel for the minimum time 20 h (corresponding to 14% water content) provides greater freezing tolerance, allowing the successful cryopreservation. Silica gel dehydration followed by immersion in LN was shown to be highly efficient for cryopreservation of seeds of G. americana , besides germinatio after thawing, high survival rates (100%) were obtained, with growth and normal establishment of the seedlings after acclimatization.","PeriodicalId":21359,"journal":{"name":"Revista Ciencia Agronomica","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71158789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Revista Ciencia Agronomica
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