Pub Date : 2022-12-20DOI: 10.15275/rusomj.2022.0401
Y. Denisenko, T. Novgorodtseva, T. Vitkina, V. Knyshova, M. Antonyuk, N. Bocharova, O. Kytikova
Background — The development of systemic inflammation is a key pathogenetic mechanism in progression of chronic obstructive pulmonary disease (COPD). Fatty acids (FAs) and their oxidized derivatives serve as essential regulators of inflammation. The relationship between systemic inflammation and FA metabolism in COPD is poorly understood. In our research, we focused on examining the FA composition of the leukocyte membrane in COPD and the FA metabolism in association with systemic inflammation. Objective — We examined 137 patients with mild, moderate, or severe COPD. The control group comprised 32 healthy non-smokers. Methods — Blood cytokines and immune cell subpopulations were evaluated by flow cytometry. The FA composition of the leukocyte membranes was analyzed by gas chromatography. The concentrations of eicosanoids (thromboxane B2 (TXB2), leukotriene B4 (LTB4)) in plasma were measured by ELISA. Results — Our results implied systemic inflammation in all patients with COPD. The analysis of the FA composition of leukocyte membrane demonstrated increased level of saturated FAs and n-6 polyunsaturated fatty acids (PUFAs), along with reduced levels of monounsaturated FAs and n-3 PUFAs, in patients with COPD. The TXB2 and LTB4 content was increasing in COPD patients. We established a significant correlation with n-6 PUFAs, immune cells, and cytokines, which was indicative of an important role of FAs in the progress of systemic inflammation in COPD. Conclusion — Thus, FA modification of immune cells in patients with chronic pathologies of the bronchopulmonary system leads not only to disruption of the cell membrane structure, but also to the pathology of immune response regulation, and contributes to the development of the inflammatory process. The latter is a decisive factor in the pathogenesis of COPD.
{"title":"Associations Of Fatty Acid Composition In Leukocyte Membranes With Systemic Inflammation In Chronic Obstructive Pulmonary Disease Progression","authors":"Y. Denisenko, T. Novgorodtseva, T. Vitkina, V. Knyshova, M. Antonyuk, N. Bocharova, O. Kytikova","doi":"10.15275/rusomj.2022.0401","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0401","url":null,"abstract":"Background — The development of systemic inflammation is a key pathogenetic mechanism in progression of chronic obstructive pulmonary disease (COPD). Fatty acids (FAs) and their oxidized derivatives serve as essential regulators of inflammation. The relationship between systemic inflammation and FA metabolism in COPD is poorly understood. In our research, we focused on examining the FA composition of the leukocyte membrane in COPD and the FA metabolism in association with systemic inflammation. Objective — We examined 137 patients with mild, moderate, or severe COPD. The control group comprised 32 healthy non-smokers. Methods — Blood cytokines and immune cell subpopulations were evaluated by flow cytometry. The FA composition of the leukocyte membranes was analyzed by gas chromatography. The concentrations of eicosanoids (thromboxane B2 (TXB2), leukotriene B4 (LTB4)) in plasma were measured by ELISA. Results — Our results implied systemic inflammation in all patients with COPD. The analysis of the FA composition of leukocyte membrane demonstrated increased level of saturated FAs and n-6 polyunsaturated fatty acids (PUFAs), along with reduced levels of monounsaturated FAs and n-3 PUFAs, in patients with COPD. The TXB2 and LTB4 content was increasing in COPD patients. We established a significant correlation with n-6 PUFAs, immune cells, and cytokines, which was indicative of an important role of FAs in the progress of systemic inflammation in COPD. Conclusion — Thus, FA modification of immune cells in patients with chronic pathologies of the bronchopulmonary system leads not only to disruption of the cell membrane structure, but also to the pathology of immune response regulation, and contributes to the development of the inflammatory process. The latter is a decisive factor in the pathogenesis of COPD.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41646543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-20DOI: 10.15275/rusomj.2022.0409
A. Tonkoglaz, E. Averyanova, Y. Barmenkova, Maryam A. Yangurazova, M. Lukyanova, V. Oleynikov
Objective — This article aims to assess parameters of myocardial electrical instability and arrhythmic events in patients after myocardial infarction (MI), with and without ST-segment elevation, comorbid/noncomorbid with a novel coronavirus infection (COVID-19) using a long-term electrocardiographic (ECG) monitoring. Methods — The study included 64 subjects: 25 (39%) patients with MI comorbid with COVID-19 (MI+C group) and 39 (61%) patients with MI noncomorbid with a novel coronavirus infection (MI group). The mean age of patients was 54.3±6.8 years. A long-term ECG monitoring for 97.4 (95% CI 77.9-115.2) hours was performed with Astrocard®-Telemetry system (Meditek JSC, Russia), starting from the 4th day of MI. Rhythm and conduction disorders, along with ischemic episodes were recorded; an analysis of ventricular late potentials, heart rate turbulence, and QT dispersion was carried out. Results — There were no differences in the frequency of delayed afterdepolarizations in MI and MI+C groups: 15-28% and 18-33% of patients, respectively. An analysis of turbulence parameters did not reveal statistically significant differences between the groups. Such arrhythmic events as frequent supraventricular extrasystole and life-threatening arrhythmias (ventricular extrasystole of grade 4A and higher sensu B. Lown and M. Wolf) were recorded significantly more often in the MI+C group than in the MI group: 48% vs. 20.5% (p=0.021) and 24% vs. 5.1% (p=0.026), respectively. Conclusion — The novel coronavirus infection (COVID-19) exacerbates myocardial electrophysiological heterogeneity in the acute cardiovascular event and is associated with an increase in clinically significant arrhythmic events.
目的:本文旨在通过长期心电图(ECG)监测，评估心肌梗死(MI)患者心肌电不稳定和心律失常事件的参数，不论有无st段抬高，合并/非合并新型冠状病毒感染(COVID-19)。方法:本研究纳入64例受试者:25例(39%)心肌梗死合并COVID-19患者(心肌梗死+C组)和39例(61%)心肌梗死合并新型冠状病毒感染患者(心肌梗死组)。患者平均年龄54.3±6.8岁。使用Astrocard®-遥测系统(Meditek JSC，俄罗斯)从心肌梗死第4天开始进行97.4 (95% CI 77.9-115.2)小时的长期心电图监测。记录心律和传导障碍以及缺血性发作;分析心室晚电位、心率动荡和QT离散度。结果- MI组和MI+C组延迟去极化频率无差异:分别为15-28%和18-33%的患者。对湍流参数的分析并没有显示两组之间有统计学上的显著差异。心肌梗死+C组发生频繁室上性心动过速和危及生命的心律失常(4A级及更高感B. low和M. Wolf室性心动过速)的频率明显高于心肌梗死组:分别为48%比20.5% (p=0.021)和24%比5.1% (p=0.026)。结论:新型冠状病毒感染(COVID-19)加剧了急性心血管事件的心肌电生理异质性，并与临床显著性心律失常事件的增加有关。
{"title":"Parameters Of Myocardial Electrical Instability In Patients After Myocardial Infarction Comorbid With A Novel Coronavirus Infection (COVID-19)","authors":"A. Tonkoglaz, E. Averyanova, Y. Barmenkova, Maryam A. Yangurazova, M. Lukyanova, V. Oleynikov","doi":"10.15275/rusomj.2022.0409","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0409","url":null,"abstract":"Objective — This article aims to assess parameters of myocardial electrical instability and arrhythmic events in patients after myocardial infarction (MI), with and without ST-segment elevation, comorbid/noncomorbid with a novel coronavirus infection (COVID-19) using a long-term electrocardiographic (ECG) monitoring. Methods — The study included 64 subjects: 25 (39%) patients with MI comorbid with COVID-19 (MI+C group) and 39 (61%) patients with MI noncomorbid with a novel coronavirus infection (MI group). The mean age of patients was 54.3±6.8 years. A long-term ECG monitoring for 97.4 (95% CI 77.9-115.2) hours was performed with Astrocard®-Telemetry system (Meditek JSC, Russia), starting from the 4th day of MI. Rhythm and conduction disorders, along with ischemic episodes were recorded; an analysis of ventricular late potentials, heart rate turbulence, and QT dispersion was carried out. Results — There were no differences in the frequency of delayed afterdepolarizations in MI and MI+C groups: 15-28% and 18-33% of patients, respectively. An analysis of turbulence parameters did not reveal statistically significant differences between the groups. Such arrhythmic events as frequent supraventricular extrasystole and life-threatening arrhythmias (ventricular extrasystole of grade 4A and higher sensu B. Lown and M. Wolf) were recorded significantly more often in the MI+C group than in the MI group: 48% vs. 20.5% (p=0.021) and 24% vs. 5.1% (p=0.026), respectively. Conclusion — The novel coronavirus infection (COVID-19) exacerbates myocardial electrophysiological heterogeneity in the acute cardiovascular event and is associated with an increase in clinically significant arrhythmic events.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47604693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-20DOI: 10.15275/rusomj.2022.0405
N. Kolotyeva, L. Limareva, F. Gilmiyarova, V. V. Boltovskaya, P. Ilyasov, O. Gusyakova, Vasiliy V. Remizov
Rationale — The impact of metabolic pathway intermediates on biological processes and cell viability is a topic of fundamental research: functional potential of low-molecular weight biologically active compounds as regulators of metabolic processes, signal molecules, transmitters of intra- and intercellular signals are of special interest; however, this field remains poorly studied. Objective — To perform computer modeling of oxaloacetate biological activity and to evaluate its effect on human dermal fibroblast culture. Material and Methods — We predicted biological activity of oxaloacetate using in silico methods. The obtained data demonstrated that oxaloacetate could influence proliferative properties and cell culture viability of dermal fibroblasts. We studied metabolic parameters and enzymatic activity in supernatant and cell culture lysate. Cell viability was assessed via methyl tetrazolium test (MTT). Results — We established that oxaloacetate had a stimulating effect on human dermal fibroblast culture. Conclusion — Our results demonstrated the stimulating role of oxaloacetate in the form of changes in the level of metabolites and activity of enzymes, such as gamma-glutamyl transpeptidase (p=0.0019) and glycerol-3-phosphate dehydrogenase (p<0.0001). We also established that oxaloacetate increased the percentage of viable cells, compared with the control (p=0.028).
{"title":"Predicted Oxaloacetate Activity, Gene Expression And Viability Of Human Dermal Fibroblasts","authors":"N. Kolotyeva, L. Limareva, F. Gilmiyarova, V. V. Boltovskaya, P. Ilyasov, O. Gusyakova, Vasiliy V. Remizov","doi":"10.15275/rusomj.2022.0405","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0405","url":null,"abstract":"Rationale — The impact of metabolic pathway intermediates on biological processes and cell viability is a topic of fundamental research: functional potential of low-molecular weight biologically active compounds as regulators of metabolic processes, signal molecules, transmitters of intra- and intercellular signals are of special interest; however, this field remains poorly studied. Objective — To perform computer modeling of oxaloacetate biological activity and to evaluate its effect on human dermal fibroblast culture. Material and Methods — We predicted biological activity of oxaloacetate using in silico methods. The obtained data demonstrated that oxaloacetate could influence proliferative properties and cell culture viability of dermal fibroblasts. We studied metabolic parameters and enzymatic activity in supernatant and cell culture lysate. Cell viability was assessed via methyl tetrazolium test (MTT). Results — We established that oxaloacetate had a stimulating effect on human dermal fibroblast culture. Conclusion — Our results demonstrated the stimulating role of oxaloacetate in the form of changes in the level of metabolites and activity of enzymes, such as gamma-glutamyl transpeptidase (p=0.0019) and glycerol-3-phosphate dehydrogenase (p<0.0001). We also established that oxaloacetate increased the percentage of viable cells, compared with the control (p=0.028).","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41507558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-20DOI: 10.15275/rusomj.2022.0404
I. Averyanova
The objective of this study was to analyze the type and balance of the diet, examine the hormonal regulation of carbohydrate metabolism in order to identify the etiology of hyperglycemic manifestations in young people of two ethnic groups residing in the territory of Magadan Oblast. Methods — Blood serum carbohydrate parameters were measured using the ion-exchange chromatography, enzymatically enhanced chemiluminescence, and the hexokinase method. Results — We established that the homeostatic model assessment of insulin resistance (HOMA-IR) index did not exceed the reference limits, which implied that the examined young men of two ethnic groups had no insulin resistance. The indigenous subjects demonstrated statistically significantly higher values of glycosylated hemoglobin, closely approaching the upper limit of the reference range. The blood insulin level in examined young men was lowered towards the lower limit of the reference range. Against the background of detected hyperglycemic manifestations in young men of two studied groups in Magadan Oblast, their dietary intake was characterized by insufficiency and imbalance in both micronutrient and macronutrient compositions. It was established that the observed diet contained an excessive amount of mono- and disaccharides in 49% of Caucasians and 72% of natives, which was 52% and 108% higher, respectively, compared with the reference range against the pronounced deficiency of chromium, copper, and vitamin D. Conclusion — The results indicated an unbalanced diet with excessive intake of mono- and disaccharides, along with reduced insulin secretion and increased glycosylated hemoglobin, which was more pronounced in the indigenous group, probably causing hyperglycemic manifestations in the carbohydrate metabolism observed in young residents of Magadan Oblast.
{"title":"Carbohydrate Metabolism, Pancreas Functional Activity And Diet In Young Residents Of Two Ethnic Groups In The North","authors":"I. Averyanova","doi":"10.15275/rusomj.2022.0404","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0404","url":null,"abstract":"The objective of this study was to analyze the type and balance of the diet, examine the hormonal regulation of carbohydrate metabolism in order to identify the etiology of hyperglycemic manifestations in young people of two ethnic groups residing in the territory of Magadan Oblast. Methods — Blood serum carbohydrate parameters were measured using the ion-exchange chromatography, enzymatically enhanced chemiluminescence, and the hexokinase method. Results — We established that the homeostatic model assessment of insulin resistance (HOMA-IR) index did not exceed the reference limits, which implied that the examined young men of two ethnic groups had no insulin resistance. The indigenous subjects demonstrated statistically significantly higher values of glycosylated hemoglobin, closely approaching the upper limit of the reference range. The blood insulin level in examined young men was lowered towards the lower limit of the reference range. Against the background of detected hyperglycemic manifestations in young men of two studied groups in Magadan Oblast, their dietary intake was characterized by insufficiency and imbalance in both micronutrient and macronutrient compositions. It was established that the observed diet contained an excessive amount of mono- and disaccharides in 49% of Caucasians and 72% of natives, which was 52% and 108% higher, respectively, compared with the reference range against the pronounced deficiency of chromium, copper, and vitamin D. Conclusion — The results indicated an unbalanced diet with excessive intake of mono- and disaccharides, along with reduced insulin secretion and increased glycosylated hemoglobin, which was more pronounced in the indigenous group, probably causing hyperglycemic manifestations in the carbohydrate metabolism observed in young residents of Magadan Oblast.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44577657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-20DOI: 10.15275/rusomj.2022.0408
A. Sumin, Ingrid Yu. Prokashko, A. V. Shcheglova
Objective — To study the choice of coping strategies and coping intensity in healthy individuals with type D personality. Material and methods — The study included 98 students of Kemerovo State Medical University (KemSMU), 68 women and 30 men (their mean age was 19.1±2.0 years). All subjects filled out psychological questionnaires to identify type D personality (DS-14) and the choice of coping strategies (Ways of Coping Questionnaire (WCQ) and Coping Strategy Indicator (CSI)). Results — The participants were divided into a group with type D personality (n=44) and without it (n=54). Individuals with type D personality had higher scores on the Escape-Avoidance (p<0.001), Accepting Responsibility (p=0.009) and Distancing (p=0.05) scales of the WSQ questionnaire, and Avoidance strategy scale of the CSI questionnaire (p=0.007). Students with type D personality were characterized by a pronounced preference for the Escape-Avoidance strategy (p=0.000018). An increase of 1 point in the values on the Escape-Avoidance scale improved the chance of identifying type D personality by 1.15 times (p<0.001). An increase in scores on the Positive Reappraisal scale reduced the likelihood of identifying type D personality (OR 0.98; p=0.005). The Escape-Avoidance coping strategy was rather strongly associated with type D personality (AUC=0.779). Conclusion — In healthy young people with type D personality, inadequate coping strategies were notably prevalent: the Escape-Avoidance strategy identified by WCQ, and the Avoidance strategy marked by the CSI. The Escape-Avoidance strategy was independently associated with type D personality, and the Positive Reappraisal strategy was associated with the non-D type.
{"title":"Coping Strategies In Young Healthy Individuals With Type D Personality","authors":"A. Sumin, Ingrid Yu. Prokashko, A. V. Shcheglova","doi":"10.15275/rusomj.2022.0408","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0408","url":null,"abstract":"Objective — To study the choice of coping strategies and coping intensity in healthy individuals with type D personality. Material and methods — The study included 98 students of Kemerovo State Medical University (KemSMU), 68 women and 30 men (their mean age was 19.1±2.0 years). All subjects filled out psychological questionnaires to identify type D personality (DS-14) and the choice of coping strategies (Ways of Coping Questionnaire (WCQ) and Coping Strategy Indicator (CSI)). Results — The participants were divided into a group with type D personality (n=44) and without it (n=54). Individuals with type D personality had higher scores on the Escape-Avoidance (p<0.001), Accepting Responsibility (p=0.009) and Distancing (p=0.05) scales of the WSQ questionnaire, and Avoidance strategy scale of the CSI questionnaire (p=0.007). Students with type D personality were characterized by a pronounced preference for the Escape-Avoidance strategy (p=0.000018). An increase of 1 point in the values on the Escape-Avoidance scale improved the chance of identifying type D personality by 1.15 times (p<0.001). An increase in scores on the Positive Reappraisal scale reduced the likelihood of identifying type D personality (OR 0.98; p=0.005). The Escape-Avoidance coping strategy was rather strongly associated with type D personality (AUC=0.779). Conclusion — In healthy young people with type D personality, inadequate coping strategies were notably prevalent: the Escape-Avoidance strategy identified by WCQ, and the Avoidance strategy marked by the CSI. The Escape-Avoidance strategy was independently associated with type D personality, and the Positive Reappraisal strategy was associated with the non-D type.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44283655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-20DOI: 10.15275/rusomj.2022.0410
A. Sumin, A. V. Shcheglova, A. Kokov, E. N. Kachurina, O. Barbarash
Objective — To examine associations between cardio-ankle vascular index (CAVI) and coronary artery calcium (CAC) score a population sample of southwestern Siberia. Methods — From the sample of 1,620 people the final analysis included 1,316 participants 25 to 64 years of age who were enrolled in an observational cross-sectional study, Epidemiology of Cardiovascular Diseases and Their Risk Factors in the Russian Federation (ESSE-RF). Study participants were split among two groups: Group 1 with CAVI≥9.0 (n=128) and Group 2 with CAVI<9.0 (n=1,188). Prevalence of coronary artery calcification in both groups was analyzed via the Agatston method. We compared main demographic and clinical data between the groups, as well as CAC scores. Results — Elevated CAVI (≥9.0) was present in 9.7% of people included in a population sample from southwestern Siberia, and coronary artery calcification was found in 33.5% of the sample. While similar rates of minimum, mild, moderate and severe CAC score were observed in the participants with elevated and normal CAVI values, CAVI as a continuous variable was statistically significantly associated with moderate and severe CAC scores (OR 1.20, 95% CI 1.06-1.37, p=0.004). CAC score values were higher in individuals at the age of ≥50 years with pathological CAVI values (116±489 vs. 75±425 in normal CAVI, p=0.035), but not in patients under the age of 50 years (64±227 and 85±475, p=0.343). Conclusion — CAVI could possibly be used as a feasible marker before assessing the CAC score in some asymptomatic Caucasian subjects, but identifying the most appropriate methods and participants, whom it could be clearly applicable to, requires further studying.
{"title":"Relationship Between Cardio-Ankle Vascular Index And Coronary Artery Calcification In A Population Sample Of Southwestern Siberia","authors":"A. Sumin, A. V. Shcheglova, A. Kokov, E. N. Kachurina, O. Barbarash","doi":"10.15275/rusomj.2022.0410","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0410","url":null,"abstract":"Objective — To examine associations between cardio-ankle vascular index (CAVI) and coronary artery calcium (CAC) score a population sample of southwestern Siberia. Methods — From the sample of 1,620 people the final analysis included 1,316 participants 25 to 64 years of age who were enrolled in an observational cross-sectional study, Epidemiology of Cardiovascular Diseases and Their Risk Factors in the Russian Federation (ESSE-RF). Study participants were split among two groups: Group 1 with CAVI≥9.0 (n=128) and Group 2 with CAVI<9.0 (n=1,188). Prevalence of coronary artery calcification in both groups was analyzed via the Agatston method. We compared main demographic and clinical data between the groups, as well as CAC scores. Results — Elevated CAVI (≥9.0) was present in 9.7% of people included in a population sample from southwestern Siberia, and coronary artery calcification was found in 33.5% of the sample. While similar rates of minimum, mild, moderate and severe CAC score were observed in the participants with elevated and normal CAVI values, CAVI as a continuous variable was statistically significantly associated with moderate and severe CAC scores (OR 1.20, 95% CI 1.06-1.37, p=0.004). CAC score values were higher in individuals at the age of ≥50 years with pathological CAVI values (116±489 vs. 75±425 in normal CAVI, p=0.035), but not in patients under the age of 50 years (64±227 and 85±475, p=0.343). Conclusion — CAVI could possibly be used as a feasible marker before assessing the CAC score in some asymptomatic Caucasian subjects, but identifying the most appropriate methods and participants, whom it could be clearly applicable to, requires further studying.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44205183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-20DOI: 10.15275/rusomj.2022.0415
N. Polukonova, N. Navolokin, M. Baryshnikova, G. Maslyakova, A. Bucharskaya, A. Polukonova
Objective — To investigate the apoptotic activity of Gratiola officinalis L. extract on human tumor cell lines by flow cytofluorometry. Material and Methods — The extract of Gratiola officinalis L. was manufactured via our original methodology. Studies were performed on human tumor cell lines: HeLa – cervical carcinoma, Jurkat – T-cell lymphoblastic leukemia, MCF-7 – breast adenocarcinoma, A549 – lung carcinoma, PC-3 – prostate carcinoma, HCT-116 – colon carcinoma, A498 – renal carcinoma, and SK-BR-3 – human breast carcinoma. Induction of apoptosis was studied after incubating cell lines with Gratiola officinalis L. extract at a concentration of 0.9 mg/mL using the Annexin V-FITC Apoptosis Kit. Caspase-dependent apoptosis was examined on a flow cytometer using anti-caspase-3-FITC (BD) kit on the Jurkat cell line. Morphological studies of HeLa cervical carcinoma cells in the alive and dead test were performed using two stains, acridine orange and propidium iodide, at different concentrations of the extract. The statistical data processing was performed using Microsoft Office Excel software. Results — One day after their exposure to Gratiola officinalis L. extract at a concentration of 0.9 mg/mL, tumor cells were mostly in late apoptosis stage. Cytotoxic activity of Gratiola officinalis L. extract was established for all investigated tumor cell cultures but their sensitivities to the extract were different. Mechanisms of antitumor action of Gratiola officinalis L. extract were identified: we established that the extract induced caspase-dependent apoptosis in tumor cells. Conclusion — The identified mechanisms of apoptotic activity of Gratiola officinalis L. extract confirmed the prospects of bioflavonoids as new-generation antitumor agents.
{"title":"Examining Apoptotic Activity Of Gratiola Officinalis L. (Scrophulariaceae) Extract On Cultured Human Tumor Cell Lines","authors":"N. Polukonova, N. Navolokin, M. Baryshnikova, G. Maslyakova, A. Bucharskaya, A. Polukonova","doi":"10.15275/rusomj.2022.0415","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0415","url":null,"abstract":"Objective — To investigate the apoptotic activity of Gratiola officinalis L. extract on human tumor cell lines by flow cytofluorometry. Material and Methods — The extract of Gratiola officinalis L. was manufactured via our original methodology. Studies were performed on human tumor cell lines: HeLa – cervical carcinoma, Jurkat – T-cell lymphoblastic leukemia, MCF-7 – breast adenocarcinoma, A549 – lung carcinoma, PC-3 – prostate carcinoma, HCT-116 – colon carcinoma, A498 – renal carcinoma, and SK-BR-3 – human breast carcinoma. Induction of apoptosis was studied after incubating cell lines with Gratiola officinalis L. extract at a concentration of 0.9 mg/mL using the Annexin V-FITC Apoptosis Kit. Caspase-dependent apoptosis was examined on a flow cytometer using anti-caspase-3-FITC (BD) kit on the Jurkat cell line. Morphological studies of HeLa cervical carcinoma cells in the alive and dead test were performed using two stains, acridine orange and propidium iodide, at different concentrations of the extract. The statistical data processing was performed using Microsoft Office Excel software. Results — One day after their exposure to Gratiola officinalis L. extract at a concentration of 0.9 mg/mL, tumor cells were mostly in late apoptosis stage. Cytotoxic activity of Gratiola officinalis L. extract was established for all investigated tumor cell cultures but their sensitivities to the extract were different. Mechanisms of antitumor action of Gratiola officinalis L. extract were identified: we established that the extract induced caspase-dependent apoptosis in tumor cells. Conclusion — The identified mechanisms of apoptotic activity of Gratiola officinalis L. extract confirmed the prospects of bioflavonoids as new-generation antitumor agents.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48659958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-28DOI: 10.15275/rusomj.2022.0304
I. Averyanova, S. Vdovenko
Background — We studied blood microcirculation and features of capillary morphology in young men with various duration of adaptation to the environmental conditions of Northeast Russia (Magadan Oblast). Methods — We examined 211 young men 17-21 years of age who were permanent residents of the North. All study subjects were divided among four groups: Caucasian migrants from central Russia (generation 0) and those born and residing in the North, representing generations 1, 2, and 3. We investigated capillary structure and microcirculation in the eponychium of the nail bed, using a computer-based video capillaroscope, Capillaroscan-1. Results — We detected the diameter reduction in arterial and intermediate capillary segments with simultaneous absence of a similar pattern in the venous capillary segment along the gradient of generation 0 towards generation 3. We also observed a shorter mean capillary length against the background of a thicker capillary network. Conclusion — With a longer adaptation period to the environmental conditions of Northeast Russia, compensatory adaptive mechanisms in the capillary network structure and microcirculation are formed, aimed at optimizing capillary blood flow.
{"title":"Effect Of Adaptation Duration To Environmental Conditions Of The North On Morphofunctional Restructuring Of Capillary Blood Flow","authors":"I. Averyanova, S. Vdovenko","doi":"10.15275/rusomj.2022.0304","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0304","url":null,"abstract":"Background — We studied blood microcirculation and features of capillary morphology in young men with various duration of adaptation to the environmental conditions of Northeast Russia (Magadan Oblast). Methods — We examined 211 young men 17-21 years of age who were permanent residents of the North. All study subjects were divided among four groups: Caucasian migrants from central Russia (generation 0) and those born and residing in the North, representing generations 1, 2, and 3. We investigated capillary structure and microcirculation in the eponychium of the nail bed, using a computer-based video capillaroscope, Capillaroscan-1. Results — We detected the diameter reduction in arterial and intermediate capillary segments with simultaneous absence of a similar pattern in the venous capillary segment along the gradient of generation 0 towards generation 3. We also observed a shorter mean capillary length against the background of a thicker capillary network. Conclusion — With a longer adaptation period to the environmental conditions of Northeast Russia, compensatory adaptive mechanisms in the capillary network structure and microcirculation are formed, aimed at optimizing capillary blood flow.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46813738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-28DOI: 10.15275/rusomj.2022.0314
S. K. Al-Hadraawy, A. H. Abood, R. Zabibah, Ameer A. Imarah, A. Almulla, Ali Abbas Abo Algon
The most frequent intestinal parasites that cause severe disorders in humans are Giardia lamblia and Entamoeba histolytica, which alter serum concentrations of deferent markers due to virulence factors and pathogenicity. A large number of people with infection are asymptomatic, and they can go for up to a year without showing any signs or symptoms. Additionally, due to prolonged diarrhoea but not acute diarrhoea, these parasites can cause malnutrition, weight loss, growth delay, and possibly low cognitive development. The aim of this study is to look at how giardiasis and amoebiasis affect the levels of certain biomarkers in the blood.
{"title":"Role Of P-Selectin, Hemopexin, Lactoferrin, Iron And Ferritin In Patients With Giardiasis And Amoebiasis: A Narrative Review","authors":"S. K. Al-Hadraawy, A. H. Abood, R. Zabibah, Ameer A. Imarah, A. Almulla, Ali Abbas Abo Algon","doi":"10.15275/rusomj.2022.0314","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0314","url":null,"abstract":"The most frequent intestinal parasites that cause severe disorders in humans are Giardia lamblia and Entamoeba histolytica, which alter serum concentrations of deferent markers due to virulence factors and pathogenicity. A large number of people with infection are asymptomatic, and they can go for up to a year without showing any signs or symptoms. Additionally, due to prolonged diarrhoea but not acute diarrhoea, these parasites can cause malnutrition, weight loss, growth delay, and possibly low cognitive development. The aim of this study is to look at how giardiasis and amoebiasis affect the levels of certain biomarkers in the blood.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49341082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-28DOI: 10.15275/rusomj.2022.0315
A. Kubanov, A. Karamova, V. Chikin, E. Monchakovskaya, Mariya A. Nefedova
Objective — to assess the efficacy and safety of intradermal injections of allogeneic fibroblasts into non-healing wounds in a patient with junctional epidermolysis bullosa. Material and Methods — A 49-year-old patient with intermediate junctional epidermolysis bullosa was injected intradermally into the base of non-healing wounds with 1 mL suspension of allogeneic fibroblasts, which contained 5×106 cells/mL, 10×106 cells/mL, and 20×106 cells/mL. Immunofluorescence mapping exhibited reduced β3 chain of laminin 332 and collagen XVII expression in the basement membrane area. Paired erosions were injected with 2% albumin or saline solution. Results — At two weeks after treatment, wound areas reduced significantly, or 100% re-epithelialization occurred. Collagen XVII and β3 chain expression of laminin 332 increased at the dermal-epidermal junction. Conclusion — Our findings demonstrated that intradermal injections of allogeneic fibroblasts could be an effective therapeutic approach for treating small non-healing wounds in junctional epidermolysis bullosa.
{"title":"Efficacy Of Intradermal Allogeneic Fibroblast Injections In Junctional Epidermolysis Bullosa","authors":"A. Kubanov, A. Karamova, V. Chikin, E. Monchakovskaya, Mariya A. Nefedova","doi":"10.15275/rusomj.2022.0315","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0315","url":null,"abstract":"Objective — to assess the efficacy and safety of intradermal injections of allogeneic fibroblasts into non-healing wounds in a patient with junctional epidermolysis bullosa. Material and Methods — A 49-year-old patient with intermediate junctional epidermolysis bullosa was injected intradermally into the base of non-healing wounds with 1 mL suspension of allogeneic fibroblasts, which contained 5×106 cells/mL, 10×106 cells/mL, and 20×106 cells/mL. Immunofluorescence mapping exhibited reduced β3 chain of laminin 332 and collagen XVII expression in the basement membrane area. Paired erosions were injected with 2% albumin or saline solution. Results — At two weeks after treatment, wound areas reduced significantly, or 100% re-epithelialization occurred. Collagen XVII and β3 chain expression of laminin 332 increased at the dermal-epidermal junction. Conclusion — Our findings demonstrated that intradermal injections of allogeneic fibroblasts could be an effective therapeutic approach for treating small non-healing wounds in junctional epidermolysis bullosa.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":"27 4","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41288172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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