Pub Date : 2024-03-25DOI: 10.21516/2072-0076-2024-17-1-83-90
M. A. Khrabrova, L. Katargina, N. Chesnokova, E. Denisova, O. Beznos
Purpose: to determine the content of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinases — 1 (TIMP-1) in the tear and blood serum (BS) and to analyze how these parameters correlate with the clinical course of endogenous uveitis in children.Materials and methods. 131 eyes with uveitis of 74 patients aged 3 to 17 (mean age 10.57 ± 3.29 years) were examined. The content of MMP-9 was found in 281 samples of the tear and 48 samples of BS. The dynamics of MMP-9 in the tear was studied in 55 patients (100 eyes), in BS — in 9 children. The content of TIMP-1 was studied in 173 tear samples and 25 BS samples. The dynamics of TIMP-1 was studied in the tear of 31 patients (56 eyes). The concentration of MMP-9 and TIMP-1 was determined by enzyme immunoassay (ELISA) using the kits ELISA for MMP-9/ELISA for tissue metalloproteinase inhibitor 1 (Cloud clone corporation, USA).Results. The content of MMP-9 in the tear dropped compared to the control group (p = 0.09). The highest content of MMP-9 in panuveitis was found in the tear as compared to the anterior and intermediate uveitis (p = 0.01). The highest MMP-9 concentration was found in cases of 3rd degree of proliferation, in contrast to the 1st and 2nd degrees (p = 0.16). An increased content of TIMP-1 was found in the tear in subactive/intermediate uveitis in contrast to inactive uveitis (p=0.08). An imbalance of MMP-9 was revealed in relation to TIMP-1 in the tears. In the early postoperative period, an increase in the content of MMP-9 and a decrease in TIMP-1 in the tear returning to the initial level was noted, which corresponds to normal wound healing.Conclusion. An increase in the content of TIMP-1 in the tear is associated with subactive/intermediate uveitis. The content of MMP-9 in the tear correlates with the proliferative process stage. A higher MMP-9 content in the tear in panuveitis, in contrast to the anterior and intermediate uveitis is associated with the involvement of all sections of the choroid of the eye into the inflammatory process. The decrease in the content of MMP-9 in the tears is probably explained by the inhibitory effect of glucocorticosteroid (GCS) therapy.
{"title":"The role of matrix metalloproteinase-9 and the tissue inhibitor of metalloproteinase — 1 in endogenous uveitis in children","authors":"M. A. Khrabrova, L. Katargina, N. Chesnokova, E. Denisova, O. Beznos","doi":"10.21516/2072-0076-2024-17-1-83-90","DOIUrl":"https://doi.org/10.21516/2072-0076-2024-17-1-83-90","url":null,"abstract":"Purpose: to determine the content of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinases — 1 (TIMP-1) in the tear and blood serum (BS) and to analyze how these parameters correlate with the clinical course of endogenous uveitis in children.Materials and methods. 131 eyes with uveitis of 74 patients aged 3 to 17 (mean age 10.57 ± 3.29 years) were examined. The content of MMP-9 was found in 281 samples of the tear and 48 samples of BS. The dynamics of MMP-9 in the tear was studied in 55 patients (100 eyes), in BS — in 9 children. The content of TIMP-1 was studied in 173 tear samples and 25 BS samples. The dynamics of TIMP-1 was studied in the tear of 31 patients (56 eyes). The concentration of MMP-9 and TIMP-1 was determined by enzyme immunoassay (ELISA) using the kits ELISA for MMP-9/ELISA for tissue metalloproteinase inhibitor 1 (Cloud clone corporation, USA).Results. The content of MMP-9 in the tear dropped compared to the control group (p = 0.09). The highest content of MMP-9 in panuveitis was found in the tear as compared to the anterior and intermediate uveitis (p = 0.01). The highest MMP-9 concentration was found in cases of 3rd degree of proliferation, in contrast to the 1st and 2nd degrees (p = 0.16). An increased content of TIMP-1 was found in the tear in subactive/intermediate uveitis in contrast to inactive uveitis (p=0.08). An imbalance of MMP-9 was revealed in relation to TIMP-1 in the tears. In the early postoperative period, an increase in the content of MMP-9 and a decrease in TIMP-1 in the tear returning to the initial level was noted, which corresponds to normal wound healing.Conclusion. An increase in the content of TIMP-1 in the tear is associated with subactive/intermediate uveitis. The content of MMP-9 in the tear correlates with the proliferative process stage. A higher MMP-9 content in the tear in panuveitis, in contrast to the anterior and intermediate uveitis is associated with the involvement of all sections of the choroid of the eye into the inflammatory process. The decrease in the content of MMP-9 in the tears is probably explained by the inhibitory effect of glucocorticosteroid (GCS) therapy.","PeriodicalId":21436,"journal":{"name":"Russian Ophthalmological Journal","volume":" 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.21516/2072-0076-2024-17-1-62-67
D. A. Teuvazhukova, L. Arutyunyan, T. G. Tlupova
Purpose: to assess the functional state of the eyes in age-related macular degeneration depending on the AREDS classification stage.Material and methods. A clinical study of 60 patients (120 eyes) with the dry AMD form, aged 55.1 ± 4.2 years, involved 30 patients (60 eyes) aged 52.7 ± 3.9 years with AREDS 2 category (initial AMD stage, group1) and 30 patients (60 eyes) aged 57.9 ± 7.8 years with AREDS 3 category (intermediate AMD stage, group 2). The complex research involved visual acuity measurement, including the assessment of near vision in various illumination conditions according to the authors’ methodology, fluorescein angiography, refractometry, tonometry, optical coherence tomography (OCT), ultrasound duplex scanning of the eye; and an immunological study aimed at determining interleukins and interferons in blood serum.Results. Visual acuity was found to depend on test illumination in healthy subjects and to substantially drop at all illumination levels in AMD, especially in the 3rd stage. With the development of AMD, the activity of the immune response was recorded.The patients of groups 1 and 2 showed an increase in the inflammatory response and a depression of the anti-inflammatory response. In group 1, an increase was recorded in IL-I by 23.5% (p < 0.05), in IL5 by 25.4% (p < 0.05), and in IL8 by 19.9% (p < 0.05). In group 2, the value of these parameters exceeded the norm by 32.1, 39.5 and 25.4% (p < 0.05), respectively. The parameters of anti-inflammatory immunity (IL10 and TGF l) were lower than the reference level by 18.3 and 24.6% (p < 0.05) of patients of group 1 and by 24.3 and 30.75 (p < 0.05) of group 2. It was established that the degree of imbalance of the immune system is associated with the stage of AREDS. In group 2 patients with AREDS 3, the content of pro-inflammatory interleukins exceeded those of group 1: IL-I by 12.5% (p < 0.05), IL5 by 11.9% (p < 0.05), IL8 by 11, 2% (p < 0.05). IL10 and TGF l in group 2 were lower than in group 1 by 10.2 and 11.8% (p < 0.05).Conclusion. Near visual acuity measurement depending on test illumination may be used as a sensitive diagnostic test in AMD in patients. At an early stage of AMD development, a disorder in the immune defense is observed, taking the form of activation of the pro-inflammatory spectrum and depression of the anti-inflammatory component, as well as microcirculatory changes Homeostatic changes of the eye appear at an early stage of the disease and are progressing as the clinical picture is deteriorating. The changes are the greatest at patients with stage 3 of AREDS.
{"title":"Clinical and functional eye condition changes in the dry form of age macular degeneration depending on the AREDS stage","authors":"D. A. Teuvazhukova, L. Arutyunyan, T. G. Tlupova","doi":"10.21516/2072-0076-2024-17-1-62-67","DOIUrl":"https://doi.org/10.21516/2072-0076-2024-17-1-62-67","url":null,"abstract":"Purpose: to assess the functional state of the eyes in age-related macular degeneration depending on the AREDS classification stage.Material and methods. A clinical study of 60 patients (120 eyes) with the dry AMD form, aged 55.1 ± 4.2 years, involved 30 patients (60 eyes) aged 52.7 ± 3.9 years with AREDS 2 category (initial AMD stage, group1) and 30 patients (60 eyes) aged 57.9 ± 7.8 years with AREDS 3 category (intermediate AMD stage, group 2). The complex research involved visual acuity measurement, including the assessment of near vision in various illumination conditions according to the authors’ methodology, fluorescein angiography, refractometry, tonometry, optical coherence tomography (OCT), ultrasound duplex scanning of the eye; and an immunological study aimed at determining interleukins and interferons in blood serum.Results. Visual acuity was found to depend on test illumination in healthy subjects and to substantially drop at all illumination levels in AMD, especially in the 3rd stage. With the development of AMD, the activity of the immune response was recorded.The patients of groups 1 and 2 showed an increase in the inflammatory response and a depression of the anti-inflammatory response. In group 1, an increase was recorded in IL-I by 23.5% (p < 0.05), in IL5 by 25.4% (p < 0.05), and in IL8 by 19.9% (p < 0.05). In group 2, the value of these parameters exceeded the norm by 32.1, 39.5 and 25.4% (p < 0.05), respectively. The parameters of anti-inflammatory immunity (IL10 and TGF l) were lower than the reference level by 18.3 and 24.6% (p < 0.05) of patients of group 1 and by 24.3 and 30.75 (p < 0.05) of group 2. It was established that the degree of imbalance of the immune system is associated with the stage of AREDS. In group 2 patients with AREDS 3, the content of pro-inflammatory interleukins exceeded those of group 1: IL-I by 12.5% (p < 0.05), IL5 by 11.9% (p < 0.05), IL8 by 11, 2% (p < 0.05). IL10 and TGF l in group 2 were lower than in group 1 by 10.2 and 11.8% (p < 0.05).Conclusion. Near visual acuity measurement depending on test illumination may be used as a sensitive diagnostic test in AMD in patients. At an early stage of AMD development, a disorder in the immune defense is observed, taking the form of activation of the pro-inflammatory spectrum and depression of the anti-inflammatory component, as well as microcirculatory changes Homeostatic changes of the eye appear at an early stage of the disease and are progressing as the clinical picture is deteriorating. The changes are the greatest at patients with stage 3 of AREDS.","PeriodicalId":21436,"journal":{"name":"Russian Ophthalmological Journal","volume":" April","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140383411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.21516/2072-0076-2024-17-1-100-105
A. V. Ivanova, A. S. Sklyarova, A. T. Khandzhyana, N. Khodzhabekyan, I. V. Manukyan
Scleral rigid contact lenses efficiently correct the vision of patients with irregular corneas. Purpose. To study the features of contact correction and visual rehabilitation of patients with irregular corneal surface using individual scleral rigid contact lenses. Results. Three clinical cases of successfully selected rigid individual scleral lenses for patients with an irregular corneal surface that appeared after a penetrating keratoplasty, implantation of intrastromal corneal segments, and eye injury. The expected refractive result was achieved; high tolerance and good adaptation to lenses was revealed. Conclusion. Scleral RCL can be successfully used in patients with irregular cornea to improve visual acuity, reduce the number of aberrations, restore binocular vision and social adaptation.
{"title":"Applying individual rigid scleral contact lenses in visual rehabilitation of patients with an irregular corneal surface. Clinical cases","authors":"A. V. Ivanova, A. S. Sklyarova, A. T. Khandzhyana, N. Khodzhabekyan, I. V. Manukyan","doi":"10.21516/2072-0076-2024-17-1-100-105","DOIUrl":"https://doi.org/10.21516/2072-0076-2024-17-1-100-105","url":null,"abstract":"Scleral rigid contact lenses efficiently correct the vision of patients with irregular corneas. Purpose. To study the features of contact correction and visual rehabilitation of patients with irregular corneal surface using individual scleral rigid contact lenses. Results. Three clinical cases of successfully selected rigid individual scleral lenses for patients with an irregular corneal surface that appeared after a penetrating keratoplasty, implantation of intrastromal corneal segments, and eye injury. The expected refractive result was achieved; high tolerance and good adaptation to lenses was revealed. Conclusion. Scleral RCL can be successfully used in patients with irregular cornea to improve visual acuity, reduce the number of aberrations, restore binocular vision and social adaptation.","PeriodicalId":21436,"journal":{"name":"Russian Ophthalmological Journal","volume":" 46","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140383970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.21516/2072-0076-2024-17-1-91-94
O. D. Dzhumagulov
A previously unknown technique of observation one’s macula is presented. Depending on the illumination of the room where the observer is present, the macula is visible in whitish-gray, yellowish, blue or green.
{"title":"Observing one’s own macula","authors":"O. D. Dzhumagulov","doi":"10.21516/2072-0076-2024-17-1-91-94","DOIUrl":"https://doi.org/10.21516/2072-0076-2024-17-1-91-94","url":null,"abstract":"A previously unknown technique of observation one’s macula is presented. Depending on the illumination of the room where the observer is present, the macula is visible in whitish-gray, yellowish, blue or green.","PeriodicalId":21436,"journal":{"name":"Russian Ophthalmological Journal","volume":" 43","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.21516/2072-0076-2024-17-1-68-73
A. F. Teshev, A. V. Malyshev
Purpose: A comparative assessment of quality of life (QL) after the use of various vitrectomy (VE) technologies in patients with an advanced stage of proliferative diabetic retinopathy (ASPDR).Material and methods. We examined 132 patients aged 42 to 76 (average age 62.8 ± 2.4 years) who met the classification criteria of ASPDR: impossibility to estimate the area of neovascularization; the fundus of the eye in the posterior pole cannot be seen or can only partialy be seen using an ophthalmoscope; evidence of preretinal or vitreal hemorrhage in the posterior pole with an area of more than 4 optic discs and retinoschisis in the macular zone. All patients were divided into two groups, comparable in age, gender and visual status of the “healthy” eye: the main group (69 eyes), in which VE was performed according to the technique developed by the authors, and the control (63 eyes), in which the traditional VE technique was used. QL was assessed before surgery and 3 months after it using the VFQ-25 and QL-20 questionnaires.Results. QL was found to be higher when the new surgery technique was used: the data of VFA-25 showed a 12.9% increase (p < 0.05) and QL-20 showed a 29.2% increase (p < 0.01), which points to a higher clinical effectiveness of the newly developed surgical treatment technique than that of the traditional technique.Conclusion. The results obtained are related to the ophthalmological features of the developed surgical treatment of ASPDR: combined (phacoemulsification of cataracts + IOL + vitrectomy) one-stage intervention, lack of drainage of subretinal fluid, etc.), medication support (preliminary intravitreal administration of an angiogenesis inhibitor) and anesthesia (intervention under local anesthesia with intravenous sedation). The differences are most effectively identified using the special QL-20 questionnaire.
{"title":"Quality of life of patients with an advanced stage of proliferative diabetic retinopathy after application of various vitrectomy technologies","authors":"A. F. Teshev, A. V. Malyshev","doi":"10.21516/2072-0076-2024-17-1-68-73","DOIUrl":"https://doi.org/10.21516/2072-0076-2024-17-1-68-73","url":null,"abstract":"Purpose: A comparative assessment of quality of life (QL) after the use of various vitrectomy (VE) technologies in patients with an advanced stage of proliferative diabetic retinopathy (ASPDR).Material and methods. We examined 132 patients aged 42 to 76 (average age 62.8 ± 2.4 years) who met the classification criteria of ASPDR: impossibility to estimate the area of neovascularization; the fundus of the eye in the posterior pole cannot be seen or can only partialy be seen using an ophthalmoscope; evidence of preretinal or vitreal hemorrhage in the posterior pole with an area of more than 4 optic discs and retinoschisis in the macular zone. All patients were divided into two groups, comparable in age, gender and visual status of the “healthy” eye: the main group (69 eyes), in which VE was performed according to the technique developed by the authors, and the control (63 eyes), in which the traditional VE technique was used. QL was assessed before surgery and 3 months after it using the VFQ-25 and QL-20 questionnaires.Results. QL was found to be higher when the new surgery technique was used: the data of VFA-25 showed a 12.9% increase (p < 0.05) and QL-20 showed a 29.2% increase (p < 0.01), which points to a higher clinical effectiveness of the newly developed surgical treatment technique than that of the traditional technique.Conclusion. The results obtained are related to the ophthalmological features of the developed surgical treatment of ASPDR: combined (phacoemulsification of cataracts + IOL + vitrectomy) one-stage intervention, lack of drainage of subretinal fluid, etc.), medication support (preliminary intravitreal administration of an angiogenesis inhibitor) and anesthesia (intervention under local anesthesia with intravenous sedation). The differences are most effectively identified using the special QL-20 questionnaire.","PeriodicalId":21436,"journal":{"name":"Russian Ophthalmological Journal","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140383528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.21516/2072-0076-2024-17-1-74-82
V. Neroev, T. Pavlenko, N. Chesnokova, O. Beznos, T. D. Okhotsimskaya, A. Y. Panova
Purpose: to assess the possibility of using thioctic acid and indomethacin tin eyedrops for the therapy of early stages of retinal neurodegeneration.Material and methods. The study was performed on 20 Chinchilla rabbits, including 16 rabbits in whom the neurodegenerative process in the retina was produced by intravitreal installation of 6-hydroxydopamine (6-OHDA), 0.25 mg per eye, and 4 rabbits (8 eyes) served as control. Thioctic acid 0.5% and Indocollir 0.1% were instilled separately and in combination 5 times daily for 7 days after the injection of 6-OHDA. Pupillary light reaction was estimated and eye fundus condition analyzed. Alpha2-macroglobuline ( 2-MG) and SOD activity, dopamine and protein concentrations were measured in retinal homogenates.Results. Instillations of thioctic acid reduced the retinal damage area almost by half as compared to the untreated group (p d 0.005). Indomethacin was less effective, but the combination of the two drugs brought about a maximal reduction of the damage area (p d 0.05 against the untreated group). Both preparations normalized the disturbed pupillary light reaction. The injection of 6-OHDA caused a decrease of dopamine concentration in the retina (0.050 ± 0.009 pg/mg as against 0.095 ± 0.031 pg/mg in healthy controls, р d 0,05). After instillations of indomethacin and thioctic acid, the concentration tended to increase, and it increased significantly after treatment with their combination (0.141 ± 0.037 pg/mg, p d 0.01 as against untreated animals). The activity of 2-MG in retina decreased after a 6-OHDA injection (2.93 ± 0.42 pg/mg as against 5.5 ± 1.2 pg/mg in healthy controls, р < 0 005) just as they increased the decreased SOD (13.30 ± 5.68 U/mg in healthy controls, 5.41 ± 1.50 U/mg in untreated animals, р < 0.05). Both preparations applied separately or in combination caused a significant increase of 2-MG activity (thioctic acid — 4.19 ± 0.91 pg/mg, indomethacin — 6.95 ± 1.09 pg/mg, combination — 7.25 ± 0.63 pg/mg, р < 0.05 as against the untreated animals) and SOD activity (thioctic acid — 8.25 ± 1.27 U/mg, indomethacin — 29.84 ± 6.63 U/mg, combination — 23.99 ± 3.78 U/mg, р < 0.01 as against the untreated animals).Conclusion. The combination of indomethacin and thioctic acid in eyedrops is the most effective for the prevention of eye damage caused by 6-OHDA, which makes this method promising for the treatment of eye diseases accompanied by neurodegeneration.
{"title":"Experimental rationale for the use of thioctic acid and indomethacin in the therapy of the early stage of retinal neurodegeneration","authors":"V. Neroev, T. Pavlenko, N. Chesnokova, O. Beznos, T. D. Okhotsimskaya, A. Y. Panova","doi":"10.21516/2072-0076-2024-17-1-74-82","DOIUrl":"https://doi.org/10.21516/2072-0076-2024-17-1-74-82","url":null,"abstract":"Purpose: to assess the possibility of using thioctic acid and indomethacin tin eyedrops for the therapy of early stages of retinal neurodegeneration.Material and methods. The study was performed on 20 Chinchilla rabbits, including 16 rabbits in whom the neurodegenerative process in the retina was produced by intravitreal installation of 6-hydroxydopamine (6-OHDA), 0.25 mg per eye, and 4 rabbits (8 eyes) served as control. Thioctic acid 0.5% and Indocollir 0.1% were instilled separately and in combination 5 times daily for 7 days after the injection of 6-OHDA. Pupillary light reaction was estimated and eye fundus condition analyzed. Alpha2-macroglobuline ( 2-MG) and SOD activity, dopamine and protein concentrations were measured in retinal homogenates.Results. Instillations of thioctic acid reduced the retinal damage area almost by half as compared to the untreated group (p d 0.005). Indomethacin was less effective, but the combination of the two drugs brought about a maximal reduction of the damage area (p d 0.05 against the untreated group). Both preparations normalized the disturbed pupillary light reaction. The injection of 6-OHDA caused a decrease of dopamine concentration in the retina (0.050 ± 0.009 pg/mg as against 0.095 ± 0.031 pg/mg in healthy controls, р d 0,05). After instillations of indomethacin and thioctic acid, the concentration tended to increase, and it increased significantly after treatment with their combination (0.141 ± 0.037 pg/mg, p d 0.01 as against untreated animals). The activity of 2-MG in retina decreased after a 6-OHDA injection (2.93 ± 0.42 pg/mg as against 5.5 ± 1.2 pg/mg in healthy controls, р < 0 005) just as they increased the decreased SOD (13.30 ± 5.68 U/mg in healthy controls, 5.41 ± 1.50 U/mg in untreated animals, р < 0.05). Both preparations applied separately or in combination caused a significant increase of 2-MG activity (thioctic acid — 4.19 ± 0.91 pg/mg, indomethacin — 6.95 ± 1.09 pg/mg, combination — 7.25 ± 0.63 pg/mg, р < 0.05 as against the untreated animals) and SOD activity (thioctic acid — 8.25 ± 1.27 U/mg, indomethacin — 29.84 ± 6.63 U/mg, combination — 23.99 ± 3.78 U/mg, р < 0.01 as against the untreated animals).Conclusion. The combination of indomethacin and thioctic acid in eyedrops is the most effective for the prevention of eye damage caused by 6-OHDA, which makes this method promising for the treatment of eye diseases accompanied by neurodegeneration.","PeriodicalId":21436,"journal":{"name":"Russian Ophthalmological Journal","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-22DOI: 10.21516/2072-0076-2024-17-1-20-27
M. M. Bikbov, E. L. Usubov, G. M. Kazakbayeva, A. U. Galimov, N. I. Pokhilko, I. Valishin, A. M. Nizamutdinova
Purpose: To evaluate the efficacy and safety of nonsteroid anti-inflammatory drugs (NSAIDs) in monotherapy and as part of combination therapy with glucocorticosteroids for the prevention of postoperative inflammation after cataract phacoemulsification.Material and methods. The study included 75 patients who underwent phacoemulsification with implantation of a posterior chamber intraocular lens. Patients were divided into groups depending on anti-inflammatory treatment: A1 — nepafenac (Nepafenac-Optic), A2 — bromfenac (Broxinac), A3 — dexamethasone (Dexamethasone), B1 — Nepafenac-Optic + dexamethasone, B2 — bromfenac + Dexamethasone. Postoperative inflammation was assessed by the severity of conjunctival hyperemia, pain, Tyndall effect and retinal thickness in the macular region.Results. The severity of the pain symptom was lower in the nepafenac group (A1) as compared to groups A2 and A3 by 49 and 74 %, respectively. A strong direct correlation with the Tyndall effect was revealed, which was more pronounced in group A3 and amounted to 0.73 points. An increase in the thickness of the retina in the macular region in the early stages after surgery was observed in groups A2 and A3, on average by 30 microns. In the groups of Nonpafenac Optic and combined therapy with GCS, the thickness of the retina remained stable. Cystic macular edema in the early postoperative period was observed in groups A2 and A3 and amounted to 2.6 and 1.3 %, respectively. There was a tendency to higher IOP rates in the GCS therapy groups.Conclusion. After surgical treatment of cataracts, the Nepafenac Optic significantly relieves the pain syndrome, as compared with monotherapy with bromfenac or dexamethasone. Nepafenac-Optic helps to reduce the severity of inflammation in the anterior and posterior segments of the eye, minimizes the changes in the retinal thickness of the macular region in the postoperative period. The severity of these effects is comparable to the combination therapy of NSAIDs + GCS. Nepafenac-Optic does not increase IOP, has a more favorable safety profile than in combinations with glucocorticosteroids, and reduces the risk of postoperative complications.
{"title":"Assessment of anti-inflammatory therapy effectiveness in the early postoperative period of cataract surgery","authors":"M. M. Bikbov, E. L. Usubov, G. M. Kazakbayeva, A. U. Galimov, N. I. Pokhilko, I. Valishin, A. M. Nizamutdinova","doi":"10.21516/2072-0076-2024-17-1-20-27","DOIUrl":"https://doi.org/10.21516/2072-0076-2024-17-1-20-27","url":null,"abstract":"Purpose: To evaluate the efficacy and safety of nonsteroid anti-inflammatory drugs (NSAIDs) in monotherapy and as part of combination therapy with glucocorticosteroids for the prevention of postoperative inflammation after cataract phacoemulsification.Material and methods. The study included 75 patients who underwent phacoemulsification with implantation of a posterior chamber intraocular lens. Patients were divided into groups depending on anti-inflammatory treatment: A1 — nepafenac (Nepafenac-Optic), A2 — bromfenac (Broxinac), A3 — dexamethasone (Dexamethasone), B1 — Nepafenac-Optic + dexamethasone, B2 — bromfenac + Dexamethasone. Postoperative inflammation was assessed by the severity of conjunctival hyperemia, pain, Tyndall effect and retinal thickness in the macular region.Results. The severity of the pain symptom was lower in the nepafenac group (A1) as compared to groups A2 and A3 by 49 and 74 %, respectively. A strong direct correlation with the Tyndall effect was revealed, which was more pronounced in group A3 and amounted to 0.73 points. An increase in the thickness of the retina in the macular region in the early stages after surgery was observed in groups A2 and A3, on average by 30 microns. In the groups of Nonpafenac Optic and combined therapy with GCS, the thickness of the retina remained stable. Cystic macular edema in the early postoperative period was observed in groups A2 and A3 and amounted to 2.6 and 1.3 %, respectively. There was a tendency to higher IOP rates in the GCS therapy groups.Conclusion. After surgical treatment of cataracts, the Nepafenac Optic significantly relieves the pain syndrome, as compared with monotherapy with bromfenac or dexamethasone. Nepafenac-Optic helps to reduce the severity of inflammation in the anterior and posterior segments of the eye, minimizes the changes in the retinal thickness of the macular region in the postoperative period. The severity of these effects is comparable to the combination therapy of NSAIDs + GCS. Nepafenac-Optic does not increase IOP, has a more favorable safety profile than in combinations with glucocorticosteroids, and reduces the risk of postoperative complications.","PeriodicalId":21436,"journal":{"name":"Russian Ophthalmological Journal","volume":" 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-22DOI: 10.21516/2072-0076-2024-17-1-7-19
V. Neroev, T. N. Malishevskaya, M. P. Kharlampidi, L. A. Mikhaylova, S. Petrov, A. Zolotarev, A. Fursova, G. V. Simonenko
The high prevalence of primary open-angle glaucoma, its leading role in the structure of visual disability, and insufficient effectiveness of treatment, prevention and rehabilitation measures require new approaches to providing quality care of patients with glaucoma. It is important to analyze the epidemiological situation, human and technological resources in various regions of the Russian Federation. The paper evaluates the compliance of specialized medical care of patients with glaucoma with the approved clinical guidelines CR 96 “Primary open-angle glaucoma” in the regions of the Russian Federation monitored by the Helmholtz National Research Center for Eye Diseases, based on the material of on-site data collection visits. The epidemiological situation relating to glaucoma, the structure and performance indicators of ophthalmological service departments, preventive measures, human and material resources, and the correctness of statistical accounting and reporting were studied. In most regions, the times of examination and treatment correspond to the regional territorial programs covered by state guarantees. The cases when primary specialized care lags behind is associated with staff shortage at the outpatient level. Practically in all regions of the Russian Federation, diagnostic methods and conservative/surgical treatment techniques with proven high efficiency conform to CR 96/1 “Primary open-angle glaucoma”. Some of the regions, however, are insufficiently equipped for early diagnosis of glaucoma and monitoring its course.
{"title":"The compliance of specialized medical care of patients with glaucoma to clinical recommendations (CR 96/1 “Primary open-angle glaucoma”) in Russian Federation regions","authors":"V. Neroev, T. N. Malishevskaya, M. P. Kharlampidi, L. A. Mikhaylova, S. Petrov, A. Zolotarev, A. Fursova, G. V. Simonenko","doi":"10.21516/2072-0076-2024-17-1-7-19","DOIUrl":"https://doi.org/10.21516/2072-0076-2024-17-1-7-19","url":null,"abstract":"The high prevalence of primary open-angle glaucoma, its leading role in the structure of visual disability, and insufficient effectiveness of treatment, prevention and rehabilitation measures require new approaches to providing quality care of patients with glaucoma. It is important to analyze the epidemiological situation, human and technological resources in various regions of the Russian Federation. The paper evaluates the compliance of specialized medical care of patients with glaucoma with the approved clinical guidelines CR 96 “Primary open-angle glaucoma” in the regions of the Russian Federation monitored by the Helmholtz National Research Center for Eye Diseases, based on the material of on-site data collection visits. The epidemiological situation relating to glaucoma, the structure and performance indicators of ophthalmological service departments, preventive measures, human and material resources, and the correctness of statistical accounting and reporting were studied. In most regions, the times of examination and treatment correspond to the regional territorial programs covered by state guarantees. The cases when primary specialized care lags behind is associated with staff shortage at the outpatient level. Practically in all regions of the Russian Federation, diagnostic methods and conservative/surgical treatment techniques with proven high efficiency conform to CR 96/1 “Primary open-angle glaucoma”. Some of the regions, however, are insufficiently equipped for early diagnosis of glaucoma and monitoring its course.","PeriodicalId":21436,"journal":{"name":"Russian Ophthalmological Journal","volume":" 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-22DOI: 10.21516/2072-0076-2024-17-1-28-31
T. V. Gavrilova, A. R. Kinkulkina, A. S. Avagyan, O. A. Svitich
Purpose: to study the association of the polymorphic marker T786C of the eNOS gene with various stages of primary open-angle glaucoma (POAG).Materials and methods. Peripheral blood of 90 patients aged 56 to 89 yrs (ave. 71 yrs) with POAG stages I, II, III was tested. DNA was isolated using a set of ribosorbents (Synthol company); subsequently, a real-time PCR reaction was performed on a DT-96 amplifier using the set to determine the polymorphic marker T786C in the eNOS gene.Results. For all groups of patients, the frequency of occurrence of alleles and genotypes was calculated, and the relationship between the emergence of POAG and the presence of an unfavorable polymorphic marker was determined. The main changes were revealed at POAG stages II and III, whilst at stage I no effect of polymorphism T786C of the eNOS gene was observed.Conclusion. For the first time, a comparative assessment was made of the distribution of alleles and genotypes by the polymorphic marker T786C in the eNOS gene of patients with various POAG stages.
{"title":"The investigation of the association of alleles and genotypes of the polymorphic marker T786C of the eNOS gene at various stages of primary open-angle glaucoma","authors":"T. V. Gavrilova, A. R. Kinkulkina, A. S. Avagyan, O. A. Svitich","doi":"10.21516/2072-0076-2024-17-1-28-31","DOIUrl":"https://doi.org/10.21516/2072-0076-2024-17-1-28-31","url":null,"abstract":"Purpose: to study the association of the polymorphic marker T786C of the eNOS gene with various stages of primary open-angle glaucoma (POAG).Materials and methods. Peripheral blood of 90 patients aged 56 to 89 yrs (ave. 71 yrs) with POAG stages I, II, III was tested. DNA was isolated using a set of ribosorbents (Synthol company); subsequently, a real-time PCR reaction was performed on a DT-96 amplifier using the set to determine the polymorphic marker T786C in the eNOS gene.Results. For all groups of patients, the frequency of occurrence of alleles and genotypes was calculated, and the relationship between the emergence of POAG and the presence of an unfavorable polymorphic marker was determined. The main changes were revealed at POAG stages II and III, whilst at stage I no effect of polymorphism T786C of the eNOS gene was observed.Conclusion. For the first time, a comparative assessment was made of the distribution of alleles and genotypes by the polymorphic marker T786C in the eNOS gene of patients with various POAG stages.","PeriodicalId":21436,"journal":{"name":"Russian Ophthalmological Journal","volume":" 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-22DOI: 10.21516/2072-0076-2024-17-1-40-46
T. D. Okhotsimskaya, N. V. Neroeva, I. V. Zolnikova, N. E. Deryugina, O. I. Markelova
Retinitis pigmentosa (RP) ranks first in the structure of hereditary retinal diseases. Vasoconstriction is one of the main ophthalmoscopic indications of PR. Laser speckle flowgraphy (LSFG) is a new non-invasive method that allows quantitative assessment of blood flow in the posterior pole of the eye.Purpose: to evaluate changes in blood flow as determined by the LSFG method in the area of the optic nerve head (ONH) and the macular area in PR patients.Material and methods. The study included 16 patients with PR aged 25 to 70 years. The control group consisted of 40 healthy volunteers, comparable in gender and age. Blood flow was determined using an LSFG-RetFlow device (Nidek), the main indicator of blood flow determined was MBR (Mean Blur Rate), which was measured for the entire study area, as well as separately for large vessels, and for the microvasculature. A wide range of pulse wave parameters determined by the tool instrument was also analyzed.Results. This study revealed a significant decrease in blood flow in PR patients compared with healthy individuals in different age groups and at different stages of the disease (p d 0.05). In patients with low visual acuity, the deficit of blood flow in the macular area was found to be more pronounced.Conclusion. LSFG is an effective method for determining ocular blood flow in PR patients. The data may indicate that a decrease in blood flow is an independent pathogenetic factor in the development of PD. In the future, the method can be used to assess the effectiveness of therapy of patients with PR.
{"title":"Studying ocular blood flow in patients with retinitis pigmentosa using laser speckle flowgraphy","authors":"T. D. Okhotsimskaya, N. V. Neroeva, I. V. Zolnikova, N. E. Deryugina, O. I. Markelova","doi":"10.21516/2072-0076-2024-17-1-40-46","DOIUrl":"https://doi.org/10.21516/2072-0076-2024-17-1-40-46","url":null,"abstract":"Retinitis pigmentosa (RP) ranks first in the structure of hereditary retinal diseases. Vasoconstriction is one of the main ophthalmoscopic indications of PR. Laser speckle flowgraphy (LSFG) is a new non-invasive method that allows quantitative assessment of blood flow in the posterior pole of the eye.Purpose: to evaluate changes in blood flow as determined by the LSFG method in the area of the optic nerve head (ONH) and the macular area in PR patients.Material and methods. The study included 16 patients with PR aged 25 to 70 years. The control group consisted of 40 healthy volunteers, comparable in gender and age. Blood flow was determined using an LSFG-RetFlow device (Nidek), the main indicator of blood flow determined was MBR (Mean Blur Rate), which was measured for the entire study area, as well as separately for large vessels, and for the microvasculature. A wide range of pulse wave parameters determined by the tool instrument was also analyzed.Results. This study revealed a significant decrease in blood flow in PR patients compared with healthy individuals in different age groups and at different stages of the disease (p d 0.05). In patients with low visual acuity, the deficit of blood flow in the macular area was found to be more pronounced.Conclusion. LSFG is an effective method for determining ocular blood flow in PR patients. The data may indicate that a decrease in blood flow is an independent pathogenetic factor in the development of PD. In the future, the method can be used to assess the effectiveness of therapy of patients with PR.","PeriodicalId":21436,"journal":{"name":"Russian Ophthalmological Journal","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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