Russian Journal of Genetics最新文献

{"title":"The Methylation Level of a CpG Site in the Human Interleukin-1β Promoter Reflects Both Current and Past Inflammation","authors":"A. K. Gribkova, A. E. Bigildeev, A. K. Shaytan","doi":"10.1134/s1022795424700406","DOIUrl":"https://doi.org/10.1134/s1022795424700406","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Viral infections, including SARS-CoV-2, are accompanied by signs of systemic inflammation, which can cause long-term sequela for the patient. Time-stable changes in the organism may be caused by epigenetic shifts inherited in a series of cell divisions, in particular, by changes in the DNA methylation profile in cells of various organs and tissues in response to proinflammatory cytokines. IL1B is a key inflammatory factor, and it was shown that CpG methylation level in its promoter can change upon pro-inflammatory stimuli, and that it was associated with significant increase in IL1B expression. In particular, a specific CpG site in the promoter of the <i>IL1B</i> gene located 299 bp upstream from the transcription start site (CpG3) was previously shown to be an important player in these processes. In this study, we examined methylation/demethylation levels of this CpG3 in publicly available genome-wide methylation studies. A total of 15 dataset were analyzed that comprised data from stromal cells in normal and inflammation-associated states, immune cells of healthy young and aging donors, patients during COVID-19 and after recovery. The level of CpG3 demethylation was found to be higher in osteoarthritis samples of cartilage as compared to healthy donors in one dataset. In blood samples of patients with rheumatoid arthritis CpG3 demethylation was also found to be statistically higher than in healthy donors. In COVID-19 studies, blood samples obtained from patients with severe symptoms had higher CpG3 demethylation levels compared to samples obtained from patients with mild symptoms and controls. The level of CpG3 demethylation increased with age in healthy people as judged by whole blood samples. The same dependency was seen for in vitro cultures of mesenchymal cells obtained from healthy donors. Taken together we showed that demethylation level of a single CpG site in <i>IL1B</i> promoter increases in several cell types due to conditions associated with local and systemic inflammation, including SARS-CoV-2 infection, and in aging. These data suggest a possibility that a history of conditions associated with inflammation within an organism may be recorded, preserved, and encoded in its DNA methylation pattern. While the specificity of these “records of inflammation” is an open question, decoding the history of pathological events associated with inflammation that had been faced by the organism is an intriguing possibility.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"55 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141773861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Structure of Wolf Populations in North Eurasia: The Effect of Exclusion of Closely Related Individuals from Analysis","authors":"P. A. Kazimirov, Yu. S. Belokon, M. M. Belokon, A. Ya. Bondarev, A. V. Davydov, E. S. Zakharov, S. V. Leontyev, D. V. Politov","doi":"10.1134/s1022795424700327","DOIUrl":"https://doi.org/10.1134/s1022795424700327","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>—</h3><p>Based on 20 autosomal microsatellite markers, the results of analysis of the genetic structure and spatial autocorrelation in the populations of grey wolf (<i>Canis lupus</i> Linnaeus, 1758) on the territory of the Russian Federation and the Republic of Kazakhstan are described. Using molecular markers, hidden genealogical patterns are revealed, which have the most pronounced effect on distances up to 150 km but may be traced over much wider spaces, up to 700–1600 km. Our research has shown that identification and exclusion of closely related genotypes has limited effect on the results of the analysis of intrapopulation genetic diversity. Meanwhile, this procedure is recommended for a more adequate assessment of the population structure since it allows for streamlining some statistical approaches. Thus, the obtained data demonstrate the integrating effect of natal migration, which works against the differential effect of philopatry. Finally, it is also shown that the exclusion of closely related individuals can lead to lower values of genetic distances between populations.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"2 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141773852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of the β1-Adrenoreceptor Gene in Patients with Atrial Fibrillation before and after Surgical Treatment","authors":"V. O. Popova, E. F. Muslimova, T. Yu. Rebrova, E. A. Archakov, R. E. Batalov, S. A. Afanasiev","doi":"10.1134/s1022795424700388","DOIUrl":"https://doi.org/10.1134/s1022795424700388","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>—</h3><p>Atrial fibrillation (AF) is one of the most frequent cardiac arrhythmias and leads to heart failure. Excessive sympathetic activity aggravates arrhythmogenic processes at the cellular and tissue levels. Hypersympathicotonia leads to desensitization of β1-adrenoreceptors (β1-AR), and negative regulation develops until the synthesis of new receptors stops. Desensitization processes may be indirectly reflected in an increase or decrease in the expression of the <i>ADRB1</i> gene encoding β1-AR. The level of relative expression of the β1-AP <i>ADRB1</i> gene in leukocytes of 52 patients with different forms of AF was analyzed in dynamics: before treatment, 3 days, and 3 and 12 months after ablation. Before ablation, expression was reduced in patients with persistent AF compared to groups with other forms of AF (<i>р =</i> 0.026). The study of dynamics revealed a significant decrease in the level of expression in patients with paroxysmal AF at the point of 3 days compared with the level before ablation (<i>p =</i> 0.003), with a further increase in values to the baseline at the point of 12 months (<i>p =</i> 0.021) relative to the point of 3 days. In patients with persistent AF, the expression level significantly increased 3 months after ablation (<i>p =</i> 0.046) compared to the level before surgery. Patients with long-term persistent AF after ablation had a tendency toward a decrease in expression.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"17 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141773857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Various Subunits of the INO80 Remodeling Complex in Chromatin Repair Assembly in Yeast Saccharomyces cerevisiae","authors":"T. A. Evstyukhina, E. A. Alekseeva, I. I. Skobeleva, V. T. Peshekhonov, V. G. Korolev","doi":"10.1134/s1022795424700303","DOIUrl":"https://doi.org/10.1134/s1022795424700303","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Chromatin repair assembly is an important step in maintaining genome stability. The correct assembly of chromatin is provided by histone chaperones, whose dysfunction can lead to the development of various forms of cancer and a number of hereditary diseases in humans. The effect of remodeling factors completes chromatin repair. The yeast chromatin remodeling complex INO80 plays an important role in chromatin architecture. We used induced mutagenesis and real-time PCR to study the role of INO80 in chromatin repair assembly. In double mutants <i>ies5</i>Δ <i>hsm3</i>Δ (<i>hif1</i>Δ), defects in the structure of nucleosomes caused by mutations <i>hsm3</i>Δ and <i>hif1</i>Δ lead to hypersensitivity of cells to UV radiation and the disappearance of <i>hsm3</i>- and <i>hif1</i>-specific mutagenesis. Double mutants carrying the <i>nhp10</i>Δ mutation and <i>hsm3</i>Δ or <i>hif1</i>Δ mutations were indistinguishable from a single mutant in terms of the lethal effect of UV irradiation, but the high UV-induced mutagenesis characteristic of all mutations disappeared. Thus, it gas been found that mutations in the genes controlling the subunits of the INO80 complex can exhibit strong interactions with mutations in histone chaperone genes. The hypothesis that the Him1 protein performs a chaperone function in the process of chromatin repair assembly has been confirmed.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"94 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141774061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthropology and Genetics of the Tolyonsky Burial Ground No. 93. Udmurtia, Polomskaya Archaeological Culture","authors":"E. V. Veselovskaya, Yu. V. Rashkovskaya, A. S. Dyomin, Kh. Kh. Mustafin, I. E. Alborova","doi":"10.1134/s1022795424700248","DOIUrl":"https://doi.org/10.1134/s1022795424700248","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The problems of the relationship between the cultures of the Volga-Kama region in general and the formation of the Udmurt ethnos involve scientists of various specializations. The main task of this work is to make a comprehensive description of the burial from the Tolyonsky burial ground located on the territory of the Debessky district of Udmurtia on the right bank of the Cheptsy River. Excavations were carried out by V.A. Semenov in the 1980s. Archaeological and anthropological material is stored in the Ivanova Historical and Cultural Museum-Reserve Idnakar of the Udmurt Republic. The absolute radiocarbon dating of the sample was obtained: 1440 ± 69 years. An anthropological study of the remains revealed a predominant body type as thoracic or pectorally muscular; the living body length was 166 cm. The studied individual was distinguished by brachycephaly, large latitudinal dimensions of the face, and a protruding nose shape. A scientific restoration of the appearance by the method of M.M. Gerasimov was carried out; contour and graphic portraits (full face and profile) were presented. Taking into account the results of genetic analysis, a watercolor artistic portrait was made. For the first time, comprehensive paleogenetic studies have been carried out on a representative of the Polomskaya archaeological culture. The Y-chromosomal haplogroup N1a1a1a2b (B181) and the mitochondrial haplogroup U4 have been established with high reliability. The identified haplogroups in individual no. 93 from the Tolyonsky burial ground along the male and female lines are geographically confined to the Central Volga-Ural region, which is consistent with the results of anthropological and archaeological research. With a probability of more than 97%, the color of the iris of the eyes is predicted to be blue in this individual.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"28 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141514246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics Analysis of Non-Synonymous Single Nucleotide Polymorphisms in Human Adk Gene","authors":"P. Farrokh","doi":"10.1134/s1022795424700273","DOIUrl":"https://doi.org/10.1134/s1022795424700273","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Adenosine kinase (ADK) controls adenosine levels. Abnormal concentration of adenosine lead to multiple disorders in humans. In this study, the effect of non-synonymous single nucleotide polymorphisms (nsSNPs) in human <i>Adk</i> was evaluated on the structure and function of long and short ADK isoforms (ADK-L and ADK-S) using computational tools. Of the 244 coding nsSNPs retrived in <i>Adk</i>, 66 amino acid changes were deleterious by at least five tools: SIFT, PhD-SNP, SNPs&amp;GO, SuSPect, SNAP2, FATHMM, and PolyPhen-2. I-Mutant 2.0 and MUpro showed that among them, 26 substitutions had a strong destabilizing effect on both ADK isoforms. The conserved region and secondary structure of ADK isoforms were predicted by the ConSurf and NetSurfP-3.0 servers, respectively. The HOPE server displayed that 11 nsSNPs, due to the change in amino acid properties, had adverse effects on ADK isoforms. Docking analysis showed that L151 and F218 in ADK-L and their corresponding residues in ADK-S are located in the ligand-binding site and their mutations changed the cavity structure or ligand binding affinity. In conclusion, this study, by using computational methods, identified 11 harmful nsSNPs in human <i>Adk</i>. These predictive results facilitate the association of these nsSNPs with disease susceptibility in population studies.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"67 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141514349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Methylation in Aortic Aneurysms of Different Localizations","authors":"A. N. Kucher, S. A. Shipulina, I. A. Goncharova, M. S. Nazarenko","doi":"10.1134/s1022795424700145","DOIUrl":"https://doi.org/10.1134/s1022795424700145","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract—</h3><p>Aortic aneurysm (AA) is a life-threatening condition, and aortic rupture that is the complication of AA in the absence of emergency surgery leads to death. Genetic (more often in thoracic AA—TAA) and environmental factors (in TAA and abdominal AA—AAA) contribute to the development of AA. This review summarizes the data of scientific publications devoted to the study of DNA methylation under the influence of AA risk factors, as well as in the cells of different parts of the aorta (thoracic, abdominal) in normal and pathological conditions. Changes in DNA methylation are observed in aortic and/or blood cells in the presence of AA risk factors (arterial hypertension, smoking, age, and comorbidities). Studies of DNA methylation in TAA and AAA are few and have been conducted using different approaches to sample formation, cell sample selection, and experimental methods. However, they provide convincing evidence of the altered DNA methylation status of genes selected for study using a candidate approach (in the AAA study), as well as of different genomic regions in genome-wide DNA methylation analysis (mainly in TAA studies). Genes localized in differentially methylated regions are associated with the functioning of the cardiovascular system and are involved in cellular and metabolic processes pathogenetically significant for the development of AA. In a number of cases, the association of DNA methylation levels with clinical parameters in AA has been established. These results indicate the prospect of expanding the studies of DNA methylation in AA, including the identification of new pathogenetically significant links in AA development.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"37 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141549082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L31 Transposons of Hexacorallia: Distribution, Diversity, and Evolution","authors":"L. V. Puzakova, M. V. Puzakov, P. M. Puzakova","doi":"10.1134/s1022795424700157","DOIUrl":"https://doi.org/10.1134/s1022795424700157","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>—</h3><p>Transposable elements (TEs) of eukaryotes—retrotransposons and DNA transposons—are nucleotide sequences that can move from locus to locus of the genome, as well as between the genomes of different organisms. <i>L31</i> DNA transposons are an ancient and diverse group belonging to the large <i>IS630/Tc1/mariner</i> group. <i>L31</i> transposons are not widespread and are present in a limited number of taxa. In addition to the sequence encoding the DDE/D transposase, <i>L31</i> transposons carry another ORF (ORF2). Detailed analysis of <i>L31</i> elements in the genomes of six-rayed corals has provided detailed information on the distribution, diversity, and structure of the elements. Two large groups, <i>L31</i>-<i>duo</i> and <i>L31</i>-<i>uno</i>, were identified, differing in both catalytic domain pattern and structure. As a result of reconstruction of the evolution of <i>L31</i> transposons, it was suggested that six-rayed corals received <i>L31</i> transposons from bivalves. The split-off group <i>L31</i>-<i>uno</i> may have been obtained by mollusks as a result of horizontal transfer from corals. Studies of TE distribution and diversity in marine invertebrates will contribute to a better understanding of the evolutionary processes of TEs and their role in the evolutionary history of species.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"11 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141549083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Complete Mitochondrial Genome of the Mashen Pig (Sus scrofa)","authors":"H. Bai, L. Liu, H. Zhao, P. Li","doi":"10.1134/s1022795424700212","DOIUrl":"https://doi.org/10.1134/s1022795424700212","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Mashen pig (<i>Sus scrofa</i>) is one of the famous local breeds in China. In this study, we sequenced and reported the complete mitochondrial genome of the Mashen pig. The complete mitogenome sequence is 16 649 bp in length, containing those typical complement of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNAs, and a non-coding control region. Our new sequenced complete mitogenome of the Mashen pig will provide valuable information for the application to conservation genetics and evolutionary studies for Chinese local pig breeds.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"30 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141514243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Chronic Social Stress on the Expression of Neurotransmitter System–Associated Genes in the Hypothalamus of Male Mice","authors":"I. L. Kovalenko, A. G. Galyamina, D. A. Smagin, N. N. Kudryavtseva","doi":"10.1134/s1022795424700170","DOIUrl":"https://doi.org/10.1134/s1022795424700170","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Chronic social stress caused by repeated negative experiences in agonistic interactions induces depressive-like behavior in male mice. The aim of this study was to investigate expression changes of genes encoding proteins involved in the metabolism, receptor sensing, and transport of catecholamines, opioids, glutamate, and GABA under the influence of chronic social stress. Hypothalamus samples were analyzed using high-throughput RNA sequencing. It was shown that in the depressed animals, the expression of catecholaminergic genes <i>Adra1b</i>, <i>Adrbk1</i>, <i>Comtd1</i>, <i>Ppp1r1b</i>, <i>Sncb</i>, <i>Sncg</i>, and <i>Th</i> is increased, while the expression of genes <i>Maoa</i> and <i>Maob</i> is reduced. The expression of opioidergic and cannabinoidergic genes <i>Pdyn</i>, <i>Penk</i>, <i>Pomc</i>, <i>Pnoc</i>, <i>Ogfr</i>, and <i>Faah</i> was found to be upregulated, while that of genes <i>Oprk1</i>, <i>Opcml</i>, <i>Ogfrl1</i>, and <i>Cnr1</i> was downregulated. The expression of glutamatergic genes <i>Grik3</i>, <i>Grik4</i>, <i>Grik5</i>, <i>Grin1</i>, <i>Grm2</i>, and <i>Grm4</i> was increased, while the expression of <i>Gria3</i>, <i>Grik1</i>, <i>Grik2</i>, <i>Grin2a</i>, <i>Grin3a</i>, <i>Grm5</i>, <i>Grm8</i>, and <i>Gad2</i> was reduced. The expression of GABAergic genes <i>Gabre</i>, <i>Gabbr2</i>, and <i>Slc6a11</i> was higher, while the expression of <i>Gabra1</i>, <i>Gabra2</i>, <i>Gabra3</i>, <i>Gabrb2</i>, <i>Gabrb3</i>, <i>Gabrg1</i>, <i>Gabrg2</i>, and <i>Slc6a13</i> was lower in the depressed animals. The results suggest that the products of genes that interact with other neurotransmitter systems (<i>Th</i>, <i>Gad2</i>, <i>Gabra1</i>, <i>Gabrg2</i>, <i>Grin1</i>, and <i>Pdyn</i>) may be of interest as potential targets for the pharmacotherapy of consequences of chronic social stress.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"29 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141549100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}