Pub Date : 2024-03-10DOI: 10.1134/s1022795424010083
S. N. Kashtanov, E. S. Zakharov, M. T. Semina, N. V. Vinokurov, A. V. Vinokurov, A. A. Onokhov, P. A. Filimonov, E. A. Soloshenkova, A. A. Yuzhakov, O. K. Sergeeva, M. M. Somova, K. A. Layshev, Yu. A. Stolpovsky
Abstract
On the basis of 16 microsatellite markers, the genetic structure of the domesticated reindeer of two breeds, Evenk and Even, bred on the territory of the Central Siberian Plateau and adjacent territories, was studied. Genetic flows between modern domesticated reindeer populations of the two breeds were analyzed. Significant differences were found between the Evenk taiga and Even breeds of domestic deer. Using a historical sample from the middle of the last century, a high degree of stability in time of the gene pool of domesticated populations was revealed. It has been established that genetic flows between wild and domesticated forms in the study area are insignificant. Statistically significant genetic differences between wild and domesticated reindeer populations are shown.
摘要 以 16 个微卫星标记为基础,研究了中西伯利亚高原和邻近地区驯养的 Evenk 和 Even 两个品种驯鹿的遗传结构。分析了这两个品种的现代驯鹿种群之间的遗传流动。研究发现,Evenk 泰加鹿种和 Even 家养鹿种之间存在显著差异。利用上世纪中叶的历史样本,揭示了驯化种群基因库在时间上的高度稳定性。研究结果表明,研究地区野生鹿和驯化鹿之间的基因流动并不明显。野生驯鹿和驯化驯鹿种群之间的遗传差异在统计学上具有重要意义。
{"title":"Genetic Structure of Domesticated Reindeer (Rangifer tarandus) Populations in the Central Siberian Plateau and Adjacent Areas","authors":"S. N. Kashtanov, E. S. Zakharov, M. T. Semina, N. V. Vinokurov, A. V. Vinokurov, A. A. Onokhov, P. A. Filimonov, E. A. Soloshenkova, A. A. Yuzhakov, O. K. Sergeeva, M. M. Somova, K. A. Layshev, Yu. A. Stolpovsky","doi":"10.1134/s1022795424010083","DOIUrl":"https://doi.org/10.1134/s1022795424010083","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>On the basis of 16 microsatellite markers, the genetic structure of the domesticated reindeer of two breeds, Evenk and Even, bred on the territory of the Central Siberian Plateau and adjacent territories, was studied. Genetic flows between modern domesticated reindeer populations of the two breeds were analyzed. Significant differences were found between the Evenk taiga and Even breeds of domestic deer. Using a historical sample from the middle of the last century, a high degree of stability in time of the gene pool of domesticated populations was revealed. It has been established that genetic flows between wild and domesticated forms in the study area are insignificant. Statistically significant genetic differences between wild and domesticated reindeer populations are shown.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"3 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140098399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-10DOI: 10.1134/s1022795424010058
M. Yu. Gallyamova, K. N. Vagin, N. M. Vasilevsky, N. I. Hammadov
Abstract
The study of changes in the genetic apparatus of biological systems due to the influence of physical factors contributes to understanding the mechanisms of adaptation. The article is devoted to the analysis of the genome of the modified Escherichia coli PL-6 variant obtained as a result of repeated and gradually increasing exposure to 60Co γ rays at the Researcher facility. The integrity of the genetic material of the studied bacterial cells was checked by electrophoresis in 1.7% agarose gel. For the analysis of genome modifications, primers were designed to amplify several loci characterized by homology in a variety of E. coli strains. On the basis of the indicators of the number and size of amplified products using each of the presented primer combinations in E. coli before and after gamma irradiation, a significant change in the genome was established.
{"title":"Analysis of Genome Variability of Escherichia coli When Exposed to Ionizing Radiation","authors":"M. Yu. Gallyamova, K. N. Vagin, N. M. Vasilevsky, N. I. Hammadov","doi":"10.1134/s1022795424010058","DOIUrl":"https://doi.org/10.1134/s1022795424010058","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The study of changes in the genetic apparatus of biological systems due to the influence of physical factors contributes to understanding the mechanisms of adaptation. The article is devoted to the analysis of the genome of the modified <i>Escherichia coli</i> PL-6 variant obtained as a result of repeated and gradually increasing exposure to <sup>60</sup>Co γ rays at the Researcher facility. The integrity of the genetic material of the studied bacterial cells was checked by electrophoresis in 1.7% agarose gel. For the analysis of genome modifications, primers were designed to amplify several loci characterized by homology in a variety of <i>E. coli</i> strains. On the basis of the indicators of the number and size of amplified products using each of the presented primer combinations in <i>E. coli</i> before and after gamma irradiation, a significant change in the genome was established.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"36 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140098196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-10DOI: 10.1134/s1022795424010095
D. B. Khandhar, V. S. Thaker
Abstract
Many mutations and phenotypic variations, which often fail to be explained by nucleotide changes with hereditary consequences, are supported by epigenetic analysis. In this study, we aimed to evaluate epigenetic changes in Wild and Mutant maize samples. This study is divided into two main experiments. The first experiment is transcriptome analysis of Wild and Mutant ear and tassel and screening of the gene clusters for epigenetic functions. Followed by the second experiment, where confirmation of the up- and down-regulated selected genes was performed in the studied samples. In the second experiment, a few new mutant plants are also added for comparative analysis. The data revealed that many genes histone modification, DNA methylation, ABC transporter and genes for seed maturation and pollen physiology exhibited up or down regulation in comparison to Wild samples. The probable role of these genes in the mutation of tassel-ear and mutant tassel is discussed.
{"title":"The Itemization of Variations in the Tassel-Ear Mutant and Wild Maize (Zea mays L.) Plants: II. Changes in Epigenetic Gene Profile","authors":"D. B. Khandhar, V. S. Thaker","doi":"10.1134/s1022795424010095","DOIUrl":"https://doi.org/10.1134/s1022795424010095","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Many mutations and phenotypic variations, which often fail to be explained by nucleotide changes with hereditary consequences, are supported by epigenetic analysis. In this study, we aimed to evaluate epigenetic changes in Wild and Mutant maize samples. This study is divided into two main experiments. The first experiment is transcriptome analysis of Wild and Mutant ear and tassel and screening of the gene clusters for epigenetic functions. Followed by the second experiment, where confirmation of the up- and down-regulated selected genes was performed in the studied samples. In the second experiment, a few new mutant plants are also added for comparative analysis. The data revealed that many genes histone modification, DNA methylation, ABC transporter and genes for seed maturation and pollen physiology exhibited up or down regulation in comparison to Wild samples. The probable role of these genes in the mutation of tassel-ear and mutant tassel is discussed.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"57 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140098328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.1134/s1022795424020145
Abstract
Dairy cattle breeding is aimed at improving the productivity, mainly through the use of a limited number of breeding bulls. As a result, an increase in inbreeding is observed causing accumulation of heterozygotes-carriers of recessive lethal mutations. A rise in the number of carriers reduces the profitability of dairy farms, since the frequency of embryonic and post-embryonic mortality increases, and the fertility of cows decreases. This paper presents the results of the development of test systems for rapid and inexpensive diagnostics of genetically determined cattle diseases that are significant for animal husbandry, namely for Holstein haplotypes 3, 6 and 7. The diagnostic technology is real-time PCR using TaqMan probes. Carriers of the Holstein haplotype 3 were not found in any of the studied populations. The carrier frequencies for HH6 and HH7 were 0.95 and 1.92%, respectively. Carrier frequencies are consistent with the results of studies worldwide, however, it is worth noting that only few large-scale screening studies have been carried out, since causative loci have been mapped relatively recently.
{"title":"Development of Real-Time PCR Kits for Bovine Haplotypes HH3, HH6, HH7 Diagnosis","authors":"","doi":"10.1134/s1022795424020145","DOIUrl":"https://doi.org/10.1134/s1022795424020145","url":null,"abstract":"<span> <h3>Abstract</h3> <p>Dairy cattle breeding is aimed at improving the productivity, mainly through the use of a limited number of breeding bulls. As a result, an increase in inbreeding is observed causing accumulation of heterozygotes-carriers of recessive lethal mutations. A rise in the number of carriers reduces the profitability of dairy farms, since the frequency of embryonic and post-embryonic mortality increases, and the fertility of cows decreases. This paper presents the results of the development of test systems for rapid and inexpensive diagnostics of genetically determined cattle diseases that are significant for animal husbandry, namely for Holstein haplotypes 3, 6 and 7. The diagnostic technology is real-time PCR using TaqMan probes. Carriers of the Holstein haplotype 3 were not found in any of the studied populations. The carrier frequencies for HH6 and HH7 were 0.95 and 1.92%, respectively. Carrier frequencies are consistent with the results of studies worldwide, however, it is worth noting that only few large-scale screening studies have been carried out, since causative loci have been mapped relatively recently.</p> </span>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"22 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140098365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.1134/s1022795424020066
Abstract
The structure of genetic variability of the prickly juniper Juniperus deltoides R.P. Adams (family Cupressaceae), an important component of Mediterranean arid and semiarid ecosystems, was studied. We genotyped samples from five populations of J. deltoids located at the northeastern limit of the range in Eurasia (Western Crimea, Transcaucasia) and one Balkan population (Bulgaria) using five nuclear microsatellite loci developed for Juniperus cedrus Webb & Berthel. Average values of intrapopulation genetic diversity were found (He varies from 0.428 to 0.602) with the lowest values in the Crimean populations. Phylogenetic analyses revealed that the populations were assigned into three genetic groups: Western Crimea, Caucasus (Krasnodar krai), and Balkans (Bulgaria). Application of AMOVA to these groups showed statistically significant differentiation (9.9% of total variability, P < 0.001). The first two groups correspond to the previously identified Asian group of J. deltoides, and the third group corresponds to the Balkan group. The differentiation of the Crimean populations from geographically close Caucasian populations is shown for the first time.
{"title":"Differentiation of Juniperus deltoidеs R.P. Аdams in the Crimean-Caucasian Region according to the Variability of Microsatellite DNA Markers","authors":"","doi":"10.1134/s1022795424020066","DOIUrl":"https://doi.org/10.1134/s1022795424020066","url":null,"abstract":"<span> <h3>Abstract</h3> <p>The structure of genetic variability of the prickly juniper <em>Juniperus deltoides</em> R.P. Adams (family Cupressaceae), an important component of Mediterranean arid and semiarid ecosystems, was studied. We genotyped samples from five populations of <em>J. deltoids</em> located at the northeastern limit of the range in Eurasia (Western Crimea, Transcaucasia) and one Balkan population (Bulgaria) using five nuclear microsatellite loci developed for <em>Juniperus cedrus</em> Webb & Berthel. Average values of intrapopulation genetic diversity were found (<em>He</em> varies from 0.428 to 0.602) with the lowest values in the Crimean populations. Phylogenetic analyses revealed that the populations were assigned into three genetic groups: Western Crimea, Caucasus (Krasnodar krai), and Balkans (Bulgaria). Application of AMOVA to these groups showed statistically significant differentiation (9.9% of total variability, <em>P</em> < 0.001). The first two groups correspond to the previously identified Asian group of <em>J. deltoides</em>, and the third group corresponds to the Balkan group. The differentiation of the Crimean populations from geographically close Caucasian populations is shown for the first time.</p> </span>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"89 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140098366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-15DOI: 10.1134/s1022795423140077
Y. Y. Li, Y. N. Zhang
Abstract
Prostate cancer is a slowly progressive cancer that develops in the epithelial cells of the prostate, which is prone to bone metastasis. In recent years, with the improvement of living conditions and the extension of life expectancy, the incidence of prostate cancer has also increased year by year in China. This study mainly focuses on the regulatory mechanism of lncRNA LINC01133 (LINC01133) in the progression of prostate cancer, providing new possibilities for the development of prognostic pathways for patients. Prostate cancer patients were diagnosed according to the magnetic resonance imaging (MRI). LINC01133 level was determined by RT-qPCR assays. The clinical significance and prognostic value of LINC01133 in prostate cancer were assessed through the chi-square analysis, Kaplan-Meier curves, and multivariate Cox regression analysis. In vitro cell experiments were performed by CCK-8 and Transwell methods. Luciferase activity assay and Pearson analysis were used to explain the relationship between LINC01133 and miR-141-5p. LINC01133 was decreased in prostate cancer tissue and cell specimens, which was associated with shorter overall survival. The abnormally high expression of LINC01133 directly targeted miR-141-5p and affected the normal growth and behavior of prostate cancer cells, while miR-141-5p mimic alleviated the inhibitory ability of LINC01133 on prostate cancer cells. LINC01133 sponge miR-141-5p mediated the progression of prostate cancer, and LINC01133 may be a potential prognostic marker for prostate cancer patients.
{"title":"LncRNA LINC01133 Targeting miR-141-5p to Mediate the Progression and Ameliorate Poor Prognosis of Prostate Cancer","authors":"Y. Y. Li, Y. N. Zhang","doi":"10.1134/s1022795423140077","DOIUrl":"https://doi.org/10.1134/s1022795423140077","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Prostate cancer is a slowly progressive cancer that develops in the epithelial cells of the prostate, which is prone to bone metastasis. In recent years, with the improvement of living conditions and the extension of life expectancy, the incidence of prostate cancer has also increased year by year in China. This study mainly focuses on the regulatory mechanism of lncRNA LINC01133 (LINC01133) in the progression of prostate cancer, providing new possibilities for the development of prognostic pathways for patients. Prostate cancer patients were diagnosed according to the magnetic resonance imaging (MRI). LINC01133 level was determined by RT-qPCR assays. The clinical significance and prognostic value of LINC01133 in prostate cancer were assessed through the chi-square analysis, Kaplan-Meier curves, and multivariate Cox regression analysis. In vitro cell experiments were performed by CCK-8 and Transwell methods. Luciferase activity assay and Pearson analysis were used to explain the relationship between LINC01133 and miR-141-5p. LINC01133 was decreased in prostate cancer tissue and cell specimens, which was associated with shorter overall survival. The abnormally high expression of LINC01133 directly targeted miR-141-5p and affected the normal growth and behavior of prostate cancer cells, while miR-141-5p mimic alleviated the inhibitory ability of LINC01133 on prostate cancer cells. LINC01133 sponge miR-141-5p mediated the progression of prostate cancer, and LINC01133 may be a potential prognostic marker for prostate cancer patients.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"2 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139476958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-15DOI: 10.1134/s1022795423140119
Y. H. Si, D. H. Meng, Z. Z. Li, T. H. Hu, L. W. Teng, Z. S. Liu
Abstract
The Mongolian subspecies of Goitred Gazelle (Gazella subgutturosa hillieriana) belongs to Artiodactyla and Bovidae. It is widely distributed in the deserts of Inner Mongolia and Ningxia. At present, there are no studies on the genetic information characteristics of Mongolian subspecies. We determined the whole mtDNA genome of the Goitered Gazelle (G. s. hillieriana) from Ningxia Hui Autonomous Region, China. The complete mitochondrial genome is 16 435 bp in length and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region (D-loop). The nucleotide composition is 33.90% A, 27.30% T, 13.20% G, and 25.60% C. Phylogenetic analysis results showeda close genetic relationship between G. s. hillieriana and Gazella subgutturosa subgutturosa. This study provides basic data for the subsequent genetic analysis of bovid, and is expected to fill the gap in genetic information of G. s. hillieriana.
摘要鹅喉羚(Gazella subgutturosa hillieriana)蒙古亚种属于有蹄目、牛科。它广泛分布于内蒙古和宁夏的沙漠地区。目前,还没有关于蒙古亚种遗传信息特征的研究。我们测定了中国宁夏回族自治区鹅喉羚(G. s. hillieriana)的全mtDNA基因组。完整的线粒体基因组长度为16 435 bp,由13个蛋白编码基因、22个tRNA基因、2个rRNA基因和1个控制区(D环)组成。系统进化分析结果表明,G. s. hillieriana 与 Gazella subgutturosa subgutturosa 的遗传关系密切。这项研究为后续的牛科动物遗传分析提供了基础数据,有望填补 G. s. hillieriana 遗传信息的空白。
{"title":"The Complete Mitochondrial Genome of the Goitered Gazelle (Gazella subgutturosa hillieriana) from Ningxia Hui Autonomous Region, China","authors":"Y. H. Si, D. H. Meng, Z. Z. Li, T. H. Hu, L. W. Teng, Z. S. Liu","doi":"10.1134/s1022795423140119","DOIUrl":"https://doi.org/10.1134/s1022795423140119","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The Mongolian subspecies of Goitred Gazelle (<i>Gazella subgutturosa hillieriana</i>) belongs to Artiodactyla and Bovidae. It is widely distributed in the deserts of Inner Mongolia and Ningxia. At present, there are no studies on the genetic information characteristics of Mongolian subspecies. We determined the whole mtDNA genome of the Goitered Gazelle (<i>G. s. hillieriana</i>) from Ningxia Hui Autonomous Region, China. The complete mitochondrial genome is 16 435 bp in length and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region (D-loop). The nucleotide composition is 33.90% A, 27.30% T, 13.20% G, and 25.60% C. Phylogenetic analysis results showeda close genetic relationship between <i>G. s. hillieriana</i> and <i>Gazella subgutturosa subgutturosa</i>. This study provides basic data for the subsequent genetic analysis of bovid, and is expected to fill the gap in genetic information of <i>G. s. hillieriana</i>.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"3 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139477056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-15DOI: 10.1134/s1022795423140089
E. V. Mashkina, E. S. Muzlaeva, V. V. Volchik, A. A. Shulga, E. G. Derevyanchuk
Abstract
A high concentration of human papillomavirus (HPV) is one of the main etiological causes of cervical cancer. The formation of a clinically significant viral load involves changes in the control of the cell cycle of epithelial cells, a decrease in the effectiveness of human DNA repair and apoptosis. The aim of the work was to study the association of SIRT1 gene rs7069102, CDKN2A gene rs3088440, TP73 gene G4C14-A4T14 polymorphisms with the risk of clinically significant viral load in HPV infection. The material for the study was DNA samples isolated from epithelial cells of the urogenital tract of women (124 HPV—infected women and 121—HPV-negative (control group)). The analysis of TP73 gene G4C14-A4T14 polymorphism was performed by PCR-CTPP. The SIRT1 gene rs7069102 polymorphism was analyzed by Real-Time PCR. Identification of the CDKN2A gene allelic variants (rs3088440) was carried out by the allele-specific PCR method followed by electrophoretic detection of the results. It was found that homozygosity for the allele C of the SIRT1 gene rs7069102 polymorphism is associated with an increased risk of clinically significant viral load in HPV infection (OR = 2.59 95% CI 1.03–6.5). Two other polymorphisms (rs3088440 CDKN2A, G4C14-A4T14TP73) are registered with the same frequency among HPV-negative women and women with clinically significant HPV concentrations. The three-locus model of studied loci interaction is statistically significant (p = 0.035) and may contribute to an increase in the viral load level in HPV infection.
{"title":"Association of SIRT1, CDKN2A, TP73 Genes Polymorphisms with the Risk of Viral Load Increase in Women Infected with Human Papillomavirus","authors":"E. V. Mashkina, E. S. Muzlaeva, V. V. Volchik, A. A. Shulga, E. G. Derevyanchuk","doi":"10.1134/s1022795423140089","DOIUrl":"https://doi.org/10.1134/s1022795423140089","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>A high concentration of human papillomavirus (HPV) is one of the main etiological causes of cervical cancer. The formation of a clinically significant viral load involves changes in the control of the cell cycle of epithelial cells, a decrease in the effectiveness of human DNA repair and apoptosis. The aim of the work was to study the association of <i>SIRT1</i> gene rs7069102, <i>CDKN2A</i> gene rs3088440, <i>TP73</i> gene <i>G4C14-A4T14</i> polymorphisms with the risk of clinically significant viral load in HPV infection. The material for the study was DNA samples isolated from epithelial cells of the urogenital tract of women (124 HPV—infected women and 121—HPV-negative (control group)). The analysis of <i>TP73</i> gene G4C14-A4T14 polymorphism was performed by PCR-CTPP. The <i>SIRT1</i> gene rs7069102 polymorphism was analyzed by Real-Time PCR. Identification of the <i>CDKN2A</i> gene allelic variants (rs3088440) was carried out by the allele-specific PCR method followed by electrophoretic detection of the results. It was found that homozygosity for the allele <i>C</i> of the <i>SIRT1</i> gene rs7069102 polymorphism is associated with an increased risk of clinically significant viral load in HPV infection (OR = 2.59 95% CI 1.03–6.5). Two other polymorphisms (rs3088440 <i>CDKN2A</i>, <i>G4C14-A4T14</i> <i>TP73</i>) are registered with the same frequency among HPV-negative women and women with clinically significant HPV concentrations. The three-locus model of studied loci interaction is statistically significant (<i>p</i> = 0.035) and may contribute to an increase in the viral load level in HPV infection.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"136 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139477055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-15DOI: 10.1134/s1022795423140107
Q. Chen, J. Song, Z. Nie, G. R. Yu, L. Y. Xu, J. Zhang, Y. Jiang, W.-P. Du
Abstract
Maize is an important food and industrial material resource. We constructed a high-density genetic map using the whole-genome resequencing and specific-locus amplified fragments sequencing (SLAF-seq) techniques for the “HZS” and “X224M” elite maize inbred lines, and 196 F2 individuals from a cross between “HZS” and “X224M.” In our study, the average resequencing depth of the maternal and paternal parents was 13.30-fold and 11.94-fold, respectively. In the F2 population, the sequencing depth ranged from 9.64-fold to 45.16-fold with an average of 23.18-fold. In total, 12, 354, 021 SNPs were detected in the F2 population, and 87.78% of which were polymorphic. Among these SNPs, 2, 843, 917 (53.96%) were included in the segregation pattern aa × bb. Finally, 6, 186 SNPs were used to construct a high-density genetic map. The length of this map included all 10 chromosomes in maize and was 1, 667.36 cM, with an average distance of 0.318 cM between markers. The number of mapped SNPs in different linkage groups (LGs) ranged from 254 to 960, and the genetic distance of each LGs ranged from 157.22 to 192.72 cM. Based on the evaluation of haplotype and heat maps, the results indicated that our genetic map is of high quality, and we now have more detailed information for gene mapping and marker-assisted breeding.
{"title":"A High-Density Genetic Map Constructed for Maize (Zea mays L.) Based on Large-Scale SNP Discovery Using Whole-Genome Resequencing and Specific-Locus Amplified Fragments Sequencing (SLAF-Seq)","authors":"Q. Chen, J. Song, Z. Nie, G. R. Yu, L. Y. Xu, J. Zhang, Y. Jiang, W.-P. Du","doi":"10.1134/s1022795423140107","DOIUrl":"https://doi.org/10.1134/s1022795423140107","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Maize is an important food and industrial material resource. We constructed a high-density genetic map using the whole-genome resequencing and specific-locus amplified fragments sequencing (SLAF-seq) techniques for the “HZS” and “X224M” elite maize inbred lines, and 196 F<sub>2</sub> individuals from a cross between “HZS” and “X224M.” In our study, the average resequencing depth of the maternal and paternal parents was 13.30-fold and 11.94-fold, respectively. In the F<sub>2</sub> population, the sequencing depth ranged from 9.64-fold to 45.16-fold with an average of 23.18-fold. In total, 12, 354, 021 SNPs were detected in the F<sub>2</sub> population, and 87.78% of which were polymorphic. Among these SNPs, 2, 843, 917 (53.96%) were included in the segregation pattern aa × bb. Finally, 6, 186 SNPs were used to construct a high-density genetic map. The length of this map included all 10 chromosomes in maize and was 1, 667.36 cM, with an average distance of 0.318 cM between markers. The number of mapped SNPs in different linkage groups (LGs) ranged from 254 to 960, and the genetic distance of each LGs ranged from 157.22 to 192.72 cM. Based on the evaluation of haplotype and heat maps, the results indicated that our genetic map is of high quality, and we now have more detailed information for gene mapping and marker-assisted breeding.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"9 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139477058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-15DOI: 10.1134/s1022795423140041
A. Ghorbani, A. A. Sadeghi, P. Shawrang, M. Chamani, F. Foroudi
Abstract
We studied the effect of palmitic acid sources or n-3 and n-6 polyunsaturated fatty acids (PUFA) in the diet on the pro-inflammatory cytokine expression and the risk of ketosis in lambs received vaccination for foot and mouth disease (FMD) virus. We used 15 Sangsari male lambs with an aged 6 ± 1 month and an average weight of 42 ± 1 kg allocated randomly to three treatments: (1) PalOil group receiving a diet of calcium soap of palm oil fatty acid (FA) to receive palmitic acid (16:0), (2) SunOil group receiving a diet containing calcium soap of sunflower oil FAs to receive linoleic acid (n-6 18:2) and (3) LinOil group receiving a diet containing calcium soap of linseed oil FA to receive α-linolenic acid (n-3 18:3). The lambs received the iso-nitrogenous and iso-caloric diet for 28 days: an adaptation period of 21 days and a sampling period of 7 days. The lambs were separately housed and could drink water. The interleukin-1β (IL-1β) mRNA expression was lower in the LinOil groups than in the PalOil and SunOil groups (P< 0.05). Also, the tumor necrosis factor-α (TNFα) mRNA expression showed an increase in the PalOil group than in the SunOil and LinOil groups, and the lowest expression of TNFα mRNA was found in the LinOil group (P < 0.05). Higher glucose blood level (P < 0.05) was measured in the PalOil group than in lambs on SunOil and LinOil diets. No significant differences were observed between treatments in BHBA and cortisol blood levels (P > 0.05). Feeding an α-linolenic acid diet to lambs after vaccination against FMD improved glucose uptake, decreased the risk of ketosis, and inhibited the expression of pro-inflammatory cytokines (TNFα and IL-1).
{"title":"The Effect of Different Sources of Unsaturated Fatty Acids on the Expression of IL-1β and TNFα Genes and Blood Factors in Sangesari Lambs Vaccinated against Foot and Mouth Disease","authors":"A. Ghorbani, A. A. Sadeghi, P. Shawrang, M. Chamani, F. Foroudi","doi":"10.1134/s1022795423140041","DOIUrl":"https://doi.org/10.1134/s1022795423140041","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>We studied the effect of palmitic acid sources or n-3 and n-6 polyunsaturated fatty acids (PUFA) in the diet on the pro-inflammatory cytokine expression and the risk of ketosis in lambs received vaccination for foot and mouth disease (FMD) virus. We used 15 Sangsari male lambs with an aged 6 ± 1 month and an average weight of 42 ± 1 kg allocated randomly to three treatments: (1) PalOil group receiving a diet of calcium soap of palm oil fatty acid (FA) to receive palmitic acid (16:0), (2) SunOil group receiving a diet containing calcium soap of sunflower oil FAs to receive linoleic acid (n-6 18:2) and (3) LinOil group receiving a diet containing calcium soap of linseed oil FA to receive α-linolenic acid (n-3 18:3). The lambs received the iso-nitrogenous and iso-caloric diet for 28 days: an adaptation period of 21 days and a sampling period of 7 days. The lambs were separately housed and could drink water. The interleukin-1β (IL-1β) mRNA expression was lower in the LinOil groups than in the PalOil and SunOil groups (<i>P</i> <i><</i> 0.05). Also, the tumor necrosis factor-α (TNFα) mRNA expression showed an increase in the PalOil group than in the SunOil and LinOil groups, and the lowest expression of TNFα mRNA was found in the LinOil group (<i>P</i> < 0.05). Higher glucose blood level (<i>P</i> < 0.05) was measured in the PalOil group than in lambs on SunOil and LinOil diets. No significant differences were observed between treatments in BHBA and cortisol blood levels (<i>P</i> > 0.05). Feeding an α-linolenic acid diet to lambs after vaccination against FMD improved glucose uptake, decreased the risk of ketosis, and inhibited the expression of pro-inflammatory cytokines (TNFα and IL-1).</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"136 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139476957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}