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Genetic Structure of Domesticated Reindeer (Rangifer tarandus) Populations in the Central Siberian Plateau and Adjacent Areas 西伯利亚中部高原及邻近地区驯养驯鹿种群的遗传结构
IF 0.6 4区 生物学 Q4 GENETICS & HEREDITY Pub Date : 2024-03-10 DOI: 10.1134/s1022795424010083
S. N. Kashtanov, E. S. Zakharov, M. T. Semina, N. V. Vinokurov, A. V. Vinokurov, A. A. Onokhov, P. A. Filimonov, E. A. Soloshenkova, A. A. Yuzhakov, O. K. Sergeeva, M. M. Somova, K. A. Layshev, Yu. A. Stolpovsky

Abstract

On the basis of 16 microsatellite markers, the genetic structure of the domesticated reindeer of two breeds, Evenk and Even, bred on the territory of the Central Siberian Plateau and adjacent territories, was studied. Genetic flows between modern domesticated reindeer populations of the two breeds were analyzed. Significant differences were found between the Evenk taiga and Even breeds of domestic deer. Using a historical sample from the middle of the last century, a high degree of stability in time of the gene pool of domesticated populations was revealed. It has been established that genetic flows between wild and domesticated forms in the study area are insignificant. Statistically significant genetic differences between wild and domesticated reindeer populations are shown.

摘要 以 16 个微卫星标记为基础,研究了中西伯利亚高原和邻近地区驯养的 Evenk 和 Even 两个品种驯鹿的遗传结构。分析了这两个品种的现代驯鹿种群之间的遗传流动。研究发现,Evenk 泰加鹿种和 Even 家养鹿种之间存在显著差异。利用上世纪中叶的历史样本,揭示了驯化种群基因库在时间上的高度稳定性。研究结果表明,研究地区野生鹿和驯化鹿之间的基因流动并不明显。野生驯鹿和驯化驯鹿种群之间的遗传差异在统计学上具有重要意义。
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引用次数: 0
Analysis of Genome Variability of Escherichia coli When Exposed to Ionizing Radiation 暴露于电离辐射时大肠杆菌基因组变异性分析
IF 0.6 4区 生物学 Q4 GENETICS & HEREDITY Pub Date : 2024-03-10 DOI: 10.1134/s1022795424010058
M. Yu. Gallyamova, K. N. Vagin, N. M. Vasilevsky, N. I. Hammadov

Abstract

The study of changes in the genetic apparatus of biological systems due to the influence of physical factors contributes to understanding the mechanisms of adaptation. The article is devoted to the analysis of the genome of the modified Escherichia coli PL-6 variant obtained as a result of repeated and gradually increasing exposure to 60Co γ rays at the Researcher facility. The integrity of the genetic material of the studied bacterial cells was checked by electrophoresis in 1.7% agarose gel. For the analysis of genome modifications, primers were designed to amplify several loci characterized by homology in a variety of E. coli strains. On the basis of the indicators of the number and size of amplified products using each of the presented primer combinations in E. coli before and after gamma irradiation, a significant change in the genome was established.

摘要 研究生物系统遗传装置受物理因素影响而发生的变化有助于了解适应机制。文章专门分析了在研究者设施中反复、逐渐增加对 60Co γ 射线的暴露而获得的改良大肠杆菌 PL-6 变体的基因组。在 1.7% 琼脂糖凝胶中进行电泳,检查所研究细菌细胞遗传物质的完整性。为了分析基因组的改变,设计了引物来扩增多种大肠杆菌菌株中具有同源性特征的几个位点。根据伽马辐照前后大肠杆菌中每种引物组合扩增产物的数量和大小指标,确定了基因组的显著变化。
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引用次数: 0
The Itemization of Variations in the Tassel-Ear Mutant and Wild Maize (Zea mays L.) Plants: II. Changes in Epigenetic Gene Profile 穗耳突变体和野生玉米(Zea mays L.)植株变异的项目化:II.表观遗传基因谱的变化
IF 0.6 4区 生物学 Q4 GENETICS & HEREDITY Pub Date : 2024-03-10 DOI: 10.1134/s1022795424010095
D. B. Khandhar, V. S. Thaker

Abstract

Many mutations and phenotypic variations, which often fail to be explained by nucleotide changes with hereditary consequences, are supported by epigenetic analysis. In this study, we aimed to evaluate epigenetic changes in Wild and Mutant maize samples. This study is divided into two main experiments. The first experiment is transcriptome analysis of Wild and Mutant ear and tassel and screening of the gene clusters for epigenetic functions. Followed by the second experiment, where confirmation of the up- and down-regulated selected genes was performed in the studied samples. In the second experiment, a few new mutant plants are also added for comparative analysis. The data revealed that many genes histone modification, DNA methylation, ABC transporter and genes for seed maturation and pollen physiology exhibited up or down regulation in comparison to Wild samples. The probable role of these genes in the mutation of tassel-ear and mutant tassel is discussed.

摘要 许多突变和表型变异往往无法用具有遗传后果的核苷酸变化来解释,而表观遗传学分析则支持这些突变和表型变异。本研究旨在评估野生玉米和突变玉米样本的表观遗传学变化。本研究分为两个主要实验。第一个实验是分析野生和突变体穗和缨的转录组,筛选表观遗传功能基因簇。随后是第二项实验,对所研究样本中上调和下调的选定基因进行确认。在第二次实验中,还加入了一些新的突变植株进行比较分析。数据显示,与野生样本相比,许多基因组蛋白修饰、DNA 甲基化、ABC 转运体以及种子成熟和花粉生理基因都出现了上调或下调。本文讨论了这些基因在穗耳突变和突变穗中的可能作用。
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引用次数: 0
Development of Real-Time PCR Kits for Bovine Haplotypes HH3, HH6, HH7 Diagnosis 开发用于诊断牛单倍型 HH3、HH6 和 HH7 的实时 PCR 检测试剂盒
IF 0.6 4区 生物学 Q4 GENETICS & HEREDITY Pub Date : 2024-02-01 DOI: 10.1134/s1022795424020145

Abstract

Dairy cattle breeding is aimed at improving the productivity, mainly through the use of a limited number of breeding bulls. As a result, an increase in inbreeding is observed causing accumulation of heterozygotes-carriers of recessive lethal mutations. A rise in the number of carriers reduces the profitability of dairy farms, since the frequency of embryonic and post-embryonic mortality increases, and the fertility of cows decreases. This paper presents the results of the development of test systems for rapid and inexpensive diagnostics of genetically determined cattle diseases that are significant for animal husbandry, namely for Holstein haplotypes 3, 6 and 7. The diagnostic technology is real-time PCR using TaqMan probes. Carriers of the Holstein haplotype 3 were not found in any of the studied populations. The carrier frequencies for HH6 and HH7 were 0.95 and 1.92%, respectively. Carrier frequencies are consistent with the results of studies worldwide, however, it is worth noting that only few large-scale screening studies have been carried out, since causative loci have been mapped relatively recently.

摘要 奶牛育种的目的是提高生产力,主要是通过使用数量有限的种公牛。因此,近亲繁殖的增加导致杂合子--隐性致死突变的携带者--的积累。由于胚胎和胚胎后死亡率增加,奶牛的繁殖力下降,携带者数量的增加降低了奶牛场的盈利能力。本文介绍了针对荷斯坦单倍型 3、6 和 7 等对畜牧业具有重要意义的牛遗传病进行快速、廉价诊断的测试系统的开发成果。诊断技术是使用 TaqMan 探针的实时 PCR。在所研究的牛群中均未发现荷斯坦单倍型 3 的携带者。HH6 和 HH7 的携带者频率分别为 0.95% 和 1.92%。携带者频率与世界范围内的研究结果一致,但值得注意的是,由于致病基因位点的绘制相对较晚,因此只进行了很少的大规模筛查研究。
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引用次数: 0
Differentiation of Juniperus deltoidеs R.P. Аdams in the Crimean-Caucasian Region according to the Variability of Microsatellite DNA Markers 根据微卫星 DNA 标记的变异性区分克里米亚-高加索地区的三角桧 R.P. Аdams
IF 0.6 4区 生物学 Q4 GENETICS & HEREDITY Pub Date : 2024-02-01 DOI: 10.1134/s1022795424020066

Abstract

The structure of genetic variability of the prickly juniper Juniperus deltoides R.P. Adams (family Cupressaceae), an important component of Mediterranean arid and semiarid ecosystems, was studied. We genotyped samples from five populations of J. deltoids located at the northeastern limit of the range in Eurasia (Western Crimea, Transcaucasia) and one Balkan population (Bulgaria) using five nuclear microsatellite loci developed for Juniperus cedrus Webb & Berthel. Average values of intrapopulation genetic diversity were found (He varies from 0.428 to 0.602) with the lowest values in the Crimean populations. Phylogenetic analyses revealed that the populations were assigned into three genetic groups: Western Crimea, Caucasus (Krasnodar krai), and Balkans (Bulgaria). Application of AMOVA to these groups showed statistically significant differentiation (9.9% of total variability, P < 0.001). The first two groups correspond to the previously identified Asian group of J. deltoides, and the third group corresponds to the Balkan group. The differentiation of the Crimean populations from geographically close Caucasian populations is shown for the first time.

摘要 我们研究了地中海干旱和半干旱生态系统的重要组成部分--刺柏(Juniperus deltoides R.P. Adams)的遗传变异结构。我们使用为杜松 Webb & Berthel 开发的五个核微卫星位点,对位于欧亚大陆杜松分布区东北部(克里米亚西部、外高加索)的五个杜松种群和一个巴尔干种群(保加利亚)的样本进行了基因分型。发现种群内遗传多样性的平均值(He 从 0.428 到 0.602 不等),其中克里米亚种群的值最低。系统发育分析表明,这些种群被划分为三个遗传组:西克里米亚、高加索(克拉斯诺达尔边疆区)和巴尔干(保加利亚)。对这些组别进行AMOVA分析表明,它们之间存在显著的统计学差异(占总变异率的9.9%,P< 0.001)。前两组与之前确定的亚洲三角木群相对应,第三组与巴尔干群相对应。克里米亚种群与地理位置相近的高加索种群之间的分化首次显示出来。
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引用次数: 0
LncRNA LINC01133 Targeting miR-141-5p to Mediate the Progression and Ameliorate Poor Prognosis of Prostate Cancer LncRNA LINC01133 靶向 miR-141-5p 以介导前列腺癌的进展并改善其不良预后
IF 0.6 4区 生物学 Q4 GENETICS & HEREDITY Pub Date : 2024-01-15 DOI: 10.1134/s1022795423140077
Y. Y. Li, Y. N. Zhang

Abstract

Prostate cancer is a slowly progressive cancer that develops in the epithelial cells of the prostate, which is prone to bone metastasis. In recent years, with the improvement of living conditions and the extension of life expectancy, the incidence of prostate cancer has also increased year by year in China. This study mainly focuses on the regulatory mechanism of lncRNA LINC01133 (LINC01133) in the progression of prostate cancer, providing new possibilities for the development of prognostic pathways for patients. Prostate cancer patients were diagnosed according to the magnetic resonance imaging (MRI). LINC01133 level was determined by RT-qPCR assays. The clinical significance and prognostic value of LINC01133 in prostate cancer were assessed through the chi-square analysis, Kaplan-Meier curves, and multivariate Cox regression analysis. In vitro cell experiments were performed by CCK-8 and Transwell methods. Luciferase activity assay and Pearson analysis were used to explain the relationship between LINC01133 and miR-141-5p. LINC01133 was decreased in prostate cancer tissue and cell specimens, which was associated with shorter overall survival. The abnormally high expression of LINC01133 directly targeted miR-141-5p and affected the normal growth and behavior of prostate cancer cells, while miR-141-5p mimic alleviated the inhibitory ability of LINC01133 on prostate cancer cells. LINC01133 sponge miR-141-5p mediated the progression of prostate cancer, and LINC01133 may be a potential prognostic marker for prostate cancer patients.

摘要 前列腺癌是一种发生于前列腺上皮细胞的缓慢进展性癌症,易发生骨转移。近年来,随着生活条件的改善和人均寿命的延长,我国前列腺癌的发病率也逐年上升。本研究主要关注lncRNA LINC01133(LINC01133)在前列腺癌进展过程中的调控机制,为患者预后路径的开发提供新的可能。前列腺癌患者根据磁共振成像(MRI)确诊。通过 RT-qPCR 检测确定 LINC01133 的水平。通过秩方分析、Kaplan-Meier曲线和多变量Cox回归分析评估了LINC01133在前列腺癌中的临床意义和预后价值。体外细胞实验采用 CCK-8 和 Transwell 方法进行。荧光素酶活性测定和皮尔逊分析被用来解释LINC01133和miR-141-5p之间的关系。LINC01133在前列腺癌组织和细胞标本中的表达量下降,这与总生存期缩短有关。LINC01133的异常高表达直接靶向miR-141-5p,影响了前列腺癌细胞的正常生长和行为,而miR-141-5p模拟物则减轻了LINC01133对前列腺癌细胞的抑制能力。LINC01133海绵体miR-141-5p介导了前列腺癌的进展,LINC01133可能是前列腺癌患者的潜在预后标志物。
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引用次数: 0
The Complete Mitochondrial Genome of the Goitered Gazelle (Gazella subgutturosa hillieriana) from Ningxia Hui Autonomous Region, China 中国宁夏回族自治区鹅喉羚(Gazella subgutturosa hillieriana)的完整线粒体基因组
IF 0.6 4区 生物学 Q4 GENETICS & HEREDITY Pub Date : 2024-01-15 DOI: 10.1134/s1022795423140119
Y. H. Si, D. H. Meng, Z. Z. Li, T. H. Hu, L. W. Teng, Z. S. Liu

Abstract

The Mongolian subspecies of Goitred Gazelle (Gazella subgutturosa hillieriana) belongs to Artiodactyla and Bovidae. It is widely distributed in the deserts of Inner Mongolia and Ningxia. At present, there are no studies on the genetic information characteristics of Mongolian subspecies. We determined the whole mtDNA genome of the Goitered Gazelle (G. s. hillieriana) from Ningxia Hui Autonomous Region, China. The complete mitochondrial genome is 16 435 bp in length and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region (D-loop). The nucleotide composition is 33.90% A, 27.30% T, 13.20% G, and 25.60% C. Phylogenetic analysis results showeda close genetic relationship between G. s. hillieriana and Gazella subgutturosa subgutturosa. This study provides basic data for the subsequent genetic analysis of bovid, and is expected to fill the gap in genetic information of G. s. hillieriana.

摘要鹅喉羚(Gazella subgutturosa hillieriana)蒙古亚种属于有蹄目、牛科。它广泛分布于内蒙古和宁夏的沙漠地区。目前,还没有关于蒙古亚种遗传信息特征的研究。我们测定了中国宁夏回族自治区鹅喉羚(G. s. hillieriana)的全mtDNA基因组。完整的线粒体基因组长度为16 435 bp,由13个蛋白编码基因、22个tRNA基因、2个rRNA基因和1个控制区(D环)组成。系统进化分析结果表明,G. s. hillieriana 与 Gazella subgutturosa subgutturosa 的遗传关系密切。这项研究为后续的牛科动物遗传分析提供了基础数据,有望填补 G. s. hillieriana 遗传信息的空白。
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引用次数: 0
Association of SIRT1, CDKN2A, TP73 Genes Polymorphisms with the Risk of Viral Load Increase in Women Infected with Human Papillomavirus SIRT1、CDKN2A、TP73 基因多态性与感染人类乳头瘤病毒的妇女病毒载量增加风险的关系
IF 0.6 4区 生物学 Q4 GENETICS & HEREDITY Pub Date : 2024-01-15 DOI: 10.1134/s1022795423140089
E. V. Mashkina, E. S. Muzlaeva, V. V. Volchik, A. A. Shulga, E. G. Derevyanchuk

Abstract

A high concentration of human papillomavirus (HPV) is one of the main etiological causes of cervical cancer. The formation of a clinically significant viral load involves changes in the control of the cell cycle of epithelial cells, a decrease in the effectiveness of human DNA repair and apoptosis. The aim of the work was to study the association of SIRT1 gene rs7069102, CDKN2A gene rs3088440, TP73 gene G4C14-A4T14 polymorphisms with the risk of clinically significant viral load in HPV infection. The material for the study was DNA samples isolated from epithelial cells of the urogenital tract of women (124 HPV—infected women and 121—HPV-negative (control group)). The analysis of TP73 gene G4C14-A4T14 polymorphism was performed by PCR-CTPP. The SIRT1 gene rs7069102 polymorphism was analyzed by Real-Time PCR. Identification of the CDKN2A gene allelic variants (rs3088440) was carried out by the allele-specific PCR method followed by electrophoretic detection of the results. It was found that homozygosity for the allele C of the SIRT1 gene rs7069102 polymorphism is associated with an increased risk of clinically significant viral load in HPV infection (OR = 2.59 95% CI 1.03–6.5). Two other polymorphisms (rs3088440 CDKN2A, G4C14-A4T14 TP73) are registered with the same frequency among HPV-negative women and women with clinically significant HPV concentrations. The three-locus model of studied loci interaction is statistically significant (p = 0.035) and may contribute to an increase in the viral load level in HPV infection.

摘要 高浓度的人类乳头瘤病毒(HPV)是宫颈癌的主要病因之一。临床上显著的病毒载量的形成涉及上皮细胞细胞周期控制的变化、人类 DNA 修复有效性的降低和细胞凋亡。这项工作的目的是研究 SIRT1 基因 rs7069102、CDKN2A 基因 rs3088440、TP73 基因 G4C14-A4T14 多态性与 HPV 感染者临床显著病毒载量风险的关联。研究材料是从女性(124 名 HPV 感染女性和 121 名 HPV 阴性女性(对照组))的泌尿生殖道上皮细胞中分离出的 DNA 样本。TP73 基因 G4C14-A4T14 多态性分析采用 PCR-CTPP 方法进行。SIRT1 基因 rs7069102 多态性通过 Real-Time PCR 进行分析。CDKN2A 基因等位基因变异(rs3088440)的鉴定采用等位基因特异性 PCR 方法,然后对结果进行电泳检测。研究发现,SIRT1 基因 rs7069102 多态性等位基因 C 的同源性与 HPV 感染临床显著病毒载量风险的增加有关(OR = 2.59 95% CI 1.03-6.5)。另外两个多态性(rs3088440 CDKN2A、G4C14-A4T14 TP73)在HPV阴性妇女和HPV临床浓度显著的妇女中出现的频率相同。所研究位点相互作用的三焦点模型具有统计学意义(p = 0.035),可能有助于提高 HPV 感染的病毒载量水平。
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引用次数: 0
A High-Density Genetic Map Constructed for Maize (Zea mays L.) Based on Large-Scale SNP Discovery Using Whole-Genome Resequencing and Specific-Locus Amplified Fragments Sequencing (SLAF-Seq) 利用全基因组重测序和特定病灶扩增片段测序 (SLAF-Seq) 大规模发现 SNP,构建玉米 (Zea mays L.) 的高密度遗传图谱
IF 0.6 4区 生物学 Q4 GENETICS & HEREDITY Pub Date : 2024-01-15 DOI: 10.1134/s1022795423140107
Q. Chen, J. Song, Z. Nie, G. R. Yu, L. Y. Xu, J. Zhang, Y. Jiang, W.-P. Du

Abstract

Maize is an important food and industrial material resource. We constructed a high-density genetic map using the whole-genome resequencing and specific-locus amplified fragments sequencing (SLAF-seq) techniques for the “HZS” and “X224M” elite maize inbred lines, and 196 F2 individuals from a cross between “HZS” and “X224M.” In our study, the average resequencing depth of the maternal and paternal parents was 13.30-fold and 11.94-fold, respectively. In the F2 population, the sequencing depth ranged from 9.64-fold to 45.16-fold with an average of 23.18-fold. In total, 12, 354, 021 SNPs were detected in the F2 population, and 87.78% of which were polymorphic. Among these SNPs, 2, 843, 917 (53.96%) were included in the segregation pattern aa × bb. Finally, 6, 186 SNPs were used to construct a high-density genetic map. The length of this map included all 10 chromosomes in maize and was 1, 667.36 cM, with an average distance of 0.318 cM between markers. The number of mapped SNPs in different linkage groups (LGs) ranged from 254 to 960, and the genetic distance of each LGs ranged from 157.22 to 192.72 cM. Based on the evaluation of haplotype and heat maps, the results indicated that our genetic map is of high quality, and we now have more detailed information for gene mapping and marker-assisted breeding.

摘要 玉米是重要的粮食和工业原料资源。我们利用全基因组重测序和特定位点扩增片段测序(SLAF-seq)技术,对 "HZS "和 "X224M "玉米近交系以及 "HZS "和 "X224M "杂交的196个F2个体构建了高密度遗传图谱。在我们的研究中,母本和父本的平均重测序深度分别为 13.30 倍和 11.94 倍。在 F2 群体中,测序深度从 9.64 倍到 45.16 倍不等,平均为 23.18 倍。F2 群体中共检测到 12 354 021 个 SNPs,其中 87.78% 为多态性。在这些 SNPs 中,2, 843, 917 个(53.96%)包含在 aa × bb 的分离模式中。最后,6 186 个 SNPs 被用于构建高密度遗传图谱。该图谱包括玉米的所有 10 条染色体,长度为 1,667.36 cM,标记间的平均距离为 0.318 cM。不同连锁群(LGs)中的SNPs数量从254个到960个不等,每个LGs的遗传距离从157.22到192.72 cM不等。根据单体型图和热图的评估,结果表明我们的遗传图谱质量较高,为基因图谱绘制和标记辅助育种提供了更详细的信息。
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引用次数: 0
The Effect of Different Sources of Unsaturated Fatty Acids on the Expression of IL-1β and TNFα Genes and Blood Factors in Sangesari Lambs Vaccinated against Foot and Mouth Disease 不同来源的不饱和脂肪酸对接种口蹄疫疫苗的桑格萨里羔羊 IL-1β 和 TNFα 基因及血液因子表达的影响
IF 0.6 4区 生物学 Q4 GENETICS & HEREDITY Pub Date : 2024-01-15 DOI: 10.1134/s1022795423140041
A. Ghorbani, A. A. Sadeghi, P. Shawrang, M. Chamani, F. Foroudi

Abstract

We studied the effect of palmitic acid sources or n-3 and n-6 polyunsaturated fatty acids (PUFA) in the diet on the pro-inflammatory cytokine expression and the risk of ketosis in lambs received vaccination for foot and mouth disease (FMD) virus. We used 15 Sangsari male lambs with an aged 6 ± 1 month and an average weight of 42 ± 1 kg allocated randomly to three treatments: (1) PalOil group receiving a diet of calcium soap of palm oil fatty acid (FA) to receive palmitic acid (16:0), (2) SunOil group receiving a diet containing calcium soap of sunflower oil FAs to receive linoleic acid (n-6 18:2) and (3) LinOil group receiving a diet containing calcium soap of linseed oil FA to receive α-linolenic acid (n-3 18:3). The lambs received the iso-nitrogenous and iso-caloric diet for 28 days: an adaptation period of 21 days and a sampling period of 7 days. The lambs were separately housed and could drink water. The interleukin-1β (IL-1β) mRNA expression was lower in the LinOil groups than in the PalOil and SunOil groups (P < 0.05). Also, the tumor necrosis factor-α (TNFα) mRNA expression showed an increase in the PalOil group than in the SunOil and LinOil groups, and the lowest expression of TNFα mRNA was found in the LinOil group (P < 0.05). Higher glucose blood level (P < 0.05) was measured in the PalOil group than in lambs on SunOil and LinOil diets. No significant differences were observed between treatments in BHBA and cortisol blood levels (P > 0.05). Feeding an α-linolenic acid diet to lambs after vaccination against FMD improved glucose uptake, decreased the risk of ketosis, and inhibited the expression of pro-inflammatory cytokines (TNFα and IL-1).

摘要 我们研究了日粮中棕榈酸来源或 n-3 和 n-6 多不饱和脂肪酸 (PUFA) 对接种口蹄疫 (FMD) 病毒疫苗的羔羊促炎细胞因子表达和酮病风险的影响。我们使用了 15 只桑萨里雄性羔羊,它们的年龄为 6 ± 1 个月,平均体重为 42 ± 1 千克,随机分配到三种处理中:(1) PalOil 组接受棕榈油脂肪酸(FA)钙皂日粮,以获得棕榈酸(16:0);(2) SunOil 组接受葵花籽油脂肪酸钙皂日粮,以获得亚油酸(n-6 18:2);(3) LinOil 组接受亚麻籽油脂肪酸钙皂日粮,以获得α-亚麻酸(n-3 18:3)。羔羊接受等氮等热量日粮 28 天:适应期 21 天,采样期 7 天。羔羊分开饲养,可以饮水。LinOil 组的白细胞介素-1β(IL-1β)mRNA 表达量低于 PalOil 组和 SunOil 组(P < 0.05)。此外,PalOil 组的肿瘤坏死因子-α(TNFα)mRNA 表达量比 SunOil 组和 LinOil 组有所增加,而 LinOil 组的 TNFα mRNA 表达量最低(P < 0.05)。与食用 SunOil 和 LinOil 日粮的羔羊相比,食用 PalOil 日粮的羔羊血糖水平更高(P < 0.05)。不同处理之间的 BHBA 和皮质醇血液水平无明显差异(P > 0.05)。给接种 FMD 疫苗后的羔羊饲喂 α-亚麻酸日粮可改善葡萄糖吸收,降低酮症风险,并抑制促炎细胞因子(TNFα 和 IL-1)的表达。
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引用次数: 0
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Russian Journal of Genetics
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