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Effect of physical exercise in people with hypothyroidism: systematic review and meta-analysis. 体育锻炼对甲状腺功能减退患者的影响:系统回顾和荟萃分析。
IF 2.1 4区 医学 Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2024-01-24 DOI: 10.1080/00365513.2023.2286651
Iratxe Duñabeitia, Daniel González-Devesa, Silvia Varela-Martínez, Jose Carlos Diz-Gómez, Carlos Ayán-Pérez

This study aimed to systematically revise the available evidence on the effects of physical exercise training programmes on people with hypothyroidism. Comparative studies were searched in six electronic databases until April 2023. The Physiotherapy Evidence Database and the Methodological Index for Non-Randomized Studies were used to determine the methodological quality of the randomized controlled trials and comparative studies respectively. A total of 10 studies were found showing a low to moderate methodological quality. Most of them were performed in women with subclinical hypothyroidism. Exercise seemed to be safe, with aerobic and resistance training leading to improvements in outcomes related to physical and mental health. The performed meta-analysis with data from 120 participants indicated that exercise showed a non-significant trend towards reducing thyroid-stimulating hormone levels (Hedges'g -0.96; 95% CI -2.71; 0.79, p = 0.160; I2 = 92%). When the analysis was performed by comparing the experimental, and control groups with data from 180 participants the results remained non-significant (SMD -1.09; CI 95% -2.88; 0.70, p = 0.23; I2 = 95%). Similar findings were obtained when pooling data for FT3 and FT4 levels. Exercise does not have a significant impact on thyroid function, although its practice can lead to secondary outcomes related to physical and mental health.

本研究旨在系统地修订关于体育锻炼计划对甲状腺功能减退患者影响的现有证据。截至2023年4月,在6个电子数据库中检索了比较研究。使用物理治疗证据数据库和非随机研究方法学指数分别确定随机对照试验和比较研究的方法学质量。总共有10项研究显示出低到中等的方法学质量。大多数是在亚临床甲状腺功能减退的妇女中进行的。运动似乎是安全的,有氧和阻力训练可以改善与身心健康相关的结果。对来自120名参与者的数据进行的荟萃分析表明,运动对降低促甲状腺激素水平没有显著的趋势(Hedges'g -0.96;95% ci -2.71;0.79, p = 0.160;i2 = 92%)。当通过比较实验组和对照组180名参与者的数据进行分析时,结果仍然不显著(SMD -1.09;Ci 95% -2.88;0.70, p = 0.23;i2 = 95%)。当合并FT3和FT4水平的数据时,也得到了类似的发现。运动对甲状腺功能没有显著影响,尽管运动可能导致与身心健康相关的次要后果。
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引用次数: 0
Preanalytical impact on the accuracy of measurements of glucagon, GLP-1 and GIP in clinical trials. 分析前对临床试验中胰高血糖素、GLP-1 和 GIP 测量准确性的影响。
IF 2.1 4区 医学 Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2024-01-24 DOI: 10.1080/00365513.2023.2294470
Christine Rasmussen, Michael M Richter, Nicole J Jensen, Niklas Heinz, Bolette Hartmann, Jens J Holst, Sasha A S Kjeldsen, Nicolai J Wewer Albrechtsen

Background: Plasma concentrations of glucagon, GLP-1 and GIP are reported in numerous clinical trials as outcome measures but preanalytical guidelines are lacking. We addressed the impact of commonly used blood containers in metabolic research on measurements of glucagon, GLP-1 and GIP in humans.

Methods: Seventeen overweight individuals were subjected to an overnight fast followed by an intravenous infusion of amino acids to stimulate hormonal secretion. Blood was sampled into five containers: EDTA-coated tubes supplemented with DMSO (control), a neprilysin inhibitor, aprotinin (a kallikrein inhibitor) or a DPP-4 inhibitor, and P800 tubes. Plasma was kept on ice before and after centrifugation and stored at -80 Celsius until batch analysis using validated sandwich ELISAs or radioimmunoassays (RIA).

Results: Measures of fasting plasma glucagon did not depend on sampling containers, whether measured by ELISA or RIA. Amino acid-induced hyperglucagonemia was numerically higher when blood was collected into P800 tubes or tubes with aprotinin. The use of p800 tubes resulted in higher concentrations of GLP-1 by RIA compared to control tubes but not for measurements with sandwich ELISA. Plasma concentrations of GIP measured by ELISA were higher in control tubes and negatively affected by P800 and the addition of aprotinin.

Conclusions: The choice of blood containers impacts on measurements of plasma concentrations of glucagon, GLP-1 and GIP, and based on this study, we recommend using EDTA-coated tubes without protease inhibitors or P800 tubes for measurements of glucagon, GLP-1 and GIP in clinical trials.

背景:许多临床试验都将胰高血糖素、GLP-1 和 GIP 的血浆浓度作为结果指标进行报告,但缺乏分析前指南。我们研究了代谢研究中常用的血液容器对人体胰高血糖素、GLP-1 和 GIP 测量的影响:方法:对 17 名超重者进行一夜禁食,然后静脉注射氨基酸以刺激荷尔蒙分泌。在五个容器中采血:EDTA涂层试管中分别加入二甲基亚砜(对照组)、肾蛋白酶抑制剂、阿普罗宁(凯利克林抑制剂)或DPP-4抑制剂,以及P800试管。血浆在离心前后均置于冰上,并储存在零下 80 摄氏度的环境中,直到使用经过验证的夹心酶联免疫吸附试验或放射免疫分析法(RIA)进行批量分析:结果:无论是采用酶联免疫吸附法还是放射免疫分析法,空腹血浆胰高血糖素的测量值都与采样容器无关。用 P800 管或含阿普丁的管采血时,氨基酸诱发的高胰高血糖素血症在数量上更高。与对照试管相比,使用p800试管通过RIA测定的GLP-1浓度更高,但使用夹心ELISA测定的GLP-1浓度则不高。用酶联免疫吸附法测定的血浆中 GIP 浓度在对照试管中更高,而 P800 和添加阿普罗宁会对其产生负面影响:血液容器的选择会影响胰高血糖素、GLP-1 和 GIP 的血浆浓度测量,根据这项研究,我们建议在临床试验中使用不含蛋白酶抑制剂的 EDTA 涂层试管或 P800 试管来测量胰高血糖素、GLP-1 和 GIP。
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引用次数: 0
Evaluation of the Premier Hb9210 instrument for HbA1c determination. Premier Hb9210检测HbA1c仪器的评价
IF 2.1 4区 医学 Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2024-01-24 DOI: 10.1080/00365513.2023.2281400
David Núñez-Jurado, Jorge Montenegro-Martínez, Ricardo Rubio-Sánchez, Manuel Conde-Sánchez, Inmaculada Domínguez-Pascual

Background: Glycated hemoglobin measurements are a valuable tool for long-term blood glucose monitoring and the diagnosis of diabetes. Its widespread use has been made possible due to the development of new analytical methods with improved performances and standardization with reference materials. The aim of the present study was to evaluate the Trinity Biotech Premier Hb9210 analyzer for the measurement of HbA1c.Methods: The precision was assessed using the CLSI EP-15A3 and EP-10A3 protocols. The latter was also used to investigate linearity, carryover, and linear drift. The comparison study was performed between Premier Hb910 and Tosoh HLC-723 G8 through Passing-Bablok regression and the Bland-Altman plot. The Fleiss Kappa index was used to assess the degree of agreement. The interference of Hb variants was investigated using samples with Hb variants S, C, D, E, J, and Seville.Results: Within-run and between-run imprecision fell between 0.37% and 1.16%. No statistically significant nonlinearity, carry-over, and/or drift were observed. The resulting regression line of the Passing-Bablok analysis was y = 0.00 + 1.00x. The Pearson correlation coefficient was 0.997. In the Bland-Altman plot, the relative bias was 0.01%. The overall Fleiss Kappa index was 0.9. No interference from hemoglobin variants was observed.Conclusion: The Premier Hb9210 demonstrated a high degree of automation, reproducibility, good agreement, minimal carry-over effect, and excellent linearity across the wide range of HbA1c levels commonly found in diabetic patients and was not influenced by Hb variants.

背景:糖化血红蛋白测量是长期血糖监测和糖尿病诊断的重要工具。由于新的分析方法的发展,其性能得到了改善,并与参考物质进行了标准化,因此它的广泛使用成为可能。本研究的目的是评估Trinity Biotech Premier Hb9210分析仪测量HbA1c的效果。方法:采用CLSI EP-15A3和EP-10A3标准进行精密度评估。后者也用于调查线性,结转和线性漂移。通过pass - bablok回归和Bland-Altman图对Premier Hb910与Tosoh HLC-723 G8进行比较研究。采用Fleiss Kappa指数评价一致性程度。使用Hb变异S、C、D、E、J和Seville样本研究Hb变异的干扰。结果:运行内和运行间不精确度在0.37% ~ 1.16%之间。未观察到统计学上显著的非线性、携带和/或漂移。Passing-Bablok分析的回归线为y = 0.00 + 1.00x。Pearson相关系数为0.997。在Bland-Altman图中,相对偏差为0.01%。整体Fleiss Kappa指数为0.9。未观察到血红蛋白变异的干扰。结论:Premier Hb9210在糖尿病患者常见的广泛HbA1c水平范围内表现出高度的自动化、可重复性、良好的一致性、最小的携带效应和良好的线性,并且不受Hb变异的影响。
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引用次数: 0
Cystatin C and eGFRCKD-EPI-CysC: novel biomarkers for renal impairment diagnosis and two-year progression-free survival in multiple myeloma. 胱抑素 C 和 eGFRCKD-EPI-CysC:诊断多发性骨髓瘤肾功能损伤和两年无进展生存期的新型生物标记物。
IF 2.1 4区 医学 Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2024-01-24 DOI: 10.1080/00365513.2023.2297364
Jian Niu, Jiajia Yu, Huifang Huang, Jinfang Shi, Dong Zheng, Jun Qiu

To evaluate cystatin C (CysC) and estimation of glomerular filtration rate (GFR) calculated using the formula, CKD-EPI-CysC (eGFRCKD-EPI-CysC) for renal impairment diagnosis and predicting the prognosis of patients with multiple myeloma (MM). One hundred-fourteen patients with MM and 38 healthy individuals were recruited for the study. Data on clinical characteristics and renal function-related biochemical indicators were collected and analyzed. Patients with MM had increased levels of CysC (1.25 (0.97-2.31) vs. 0.84 (0.80-0.92), respectively, p < 0.001) and decreased levels of eGFRCKD-EPI-CysC (53.0 (24.4-81.1) vs. 97.2 (87.0-104.5), respectively, p < 0.001), compared with healthy individuals. There were significantly more patients with elevated CysC levels than with elevated sCr levels (64.9% vs. 41.2%, respectively, p < 0.001). The CKD-EPI-CysC formula detected more patients with eGFR < 60 ml/(min × 1.73 m2) than the CKD-EPI-sCr formula (52.63% vs. 37.72%, respectively, p < 0.001). Correlation analysis found that only CysC, eGFRCKD-EPI-CysC, and eGFRCKD-EPI-sCr-CysC strongly correlated with β2-microglobulin in group ISS-I. Logistic regression analysis was used to screen CysC (OR = 1.495, 95% CI = 1.097-2.038, p = 0.011) and eGFRCKD-EPI-CysC (OR = 0.980, 95% CI = 0.967-0.993, p = 0.003) as independent prognostic indicators for 2-year-progression-free survival (PFS) of patients with MM. Receiver operating characteristic curve analysis found that CysC values >1.70 mg/L had 67.6% sensitivity and 65.2% specificity and eGFRCKD-EPI-CysC values <38.62 ml/(min × 1.73 m2) had 65.2% sensitivity and 67.6% specificity for 2-year PFS of patients with MM. In summary, CysC and eGFRCKD-EPI-CysC were more sensitive than sCr and eGFRCKD-EPI-sCr for predicting renal impairment in patients newly diagnosed with MM. Increased CysC and decreased eGFRCKD-EPI-CysC levels were effective predictors of 2-year PFS of patients with MM.

目的:评估胱抑素C(CysC)和使用CKD-EPI-CysC(eGFRCKD-EPI-CysC)公式计算的肾小球滤过率(GFR)估算值,用于多发性骨髓瘤(MM)患者的肾功能损害诊断和预后预测。研究共招募了 14 名 MM 患者和 38 名健康人。研究收集并分析了临床特征和肾功能相关生化指标的数据。MM患者的CysC(1.25 (0.97-2.31) vs. 0.84 (0.80-0.92), p CKD-EPI-CysC(53.0 (24.4-81.1) vs. 97.2 (87.0-104.5),分别 p p 2)比 CKD-EPI-sCr 公式(52.63% vs. 37.72%,分别 p CKD-EPI-CysC,eGFRCKD-EPI-sCr-CysC 与 ISS-I 组的β2-微球蛋白密切相关。利用逻辑回归分析筛选出 CysC(OR = 1.495,95% CI = 1.097-2.038,p = 0.011)和 eGFRCKD-EPI-CysC(OR = 0.980,95% CI = 0.967-0.993,p = 0.003)作为 MM 患者 2 年无进展生存期(PFS)的独立预后指标。接收者操作特征曲线分析发现,CysC值>1.70 mg/L对MM患者2年无进展生存期的敏感性为67.6%,特异性为65.2%;eGFRCKD-EPI-CysC值2)对MM患者2年无进展生存期的敏感性为65.2%,特异性为67.6%。总之,CysC 和 eGFRCKD-EPI-CysC 比 sCr 和 eGFRCKD-EPI-sCr 对预测新诊断为 MM 患者的肾功能损害更敏感。CysC水平升高和eGFRCKD-EPI-CysC水平降低可有效预测MM患者的2年PFS。
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引用次数: 0
Dysmagnesemia as a predictor of developing diabetic levels of hemoglobin A1c. 作为糖尿病血红蛋白 A1c 发展水平预测因子的镁异常血症。
IF 2.1 4区 医学 Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2024-01-24 DOI: 10.1080/00365513.2024.2303707
Solav Aziz Ali, Anna Elise Engell, Bent Struer Lind, Henrik Løvendahl Jørgensen

The aim of this study was to assess the possible association between P-Mg and subsequent high levels of HbA1c. The study involves data from primary health care patients and data from patients treated in hospitals located in the capital region of Denmark. P-Mg and HbA1c levels were analyzed from 121,575 patients in the period 2010-2022. Patients were categorized in a diabetic and non-diabetic group. Out of 121,575 patients, 8,532 were categorized as diabetic. A reverse J-shaped association between P-Mg and HbA1c levels ≥ 48 mmol/mol was observed with nadir at P-Mg of 0.90 mmol/L. The unadjusted hazard ratio (HR) for having a first HbA1c measurement ≥ 48 mmol/mol is 1.54 (95% Cl 1.50; 1.57) per 0.1 mmol/L decrease in P-Mg when P-Mg is lower than 0.90 mmol/L. After adjusting for age and gender, the HR remained significant at 1.45 (95% Cl 1.41; 1.48).For P-Mg levels above 0.90 mmol/L, the unadjusted HR per 0.1 mmol/L increase in P-Mg was 1.04 (95% Cl 1.02; 1.06). After adjusting for age and gender the HR remained significant at 1.06 (95% Cl 1.05; 1.08). In conclusion, this study found that patients with dysmagnesemia have a higher risk of developing diabetes even after adjusting for age and gender. Hyper- or hypomagnesemia in patients could be a biomarker for predicting the risk of developing diabetes.

这项研究的目的是评估 P-Mg 与随后的高 HbA1c 之间可能存在的关联。研究涉及初级医疗保健患者的数据和在丹麦首都地区医院接受治疗的患者的数据。研究分析了 2010-2022 年间 121,575 名患者的 P-Mg 和 HbA1c 水平。患者被分为糖尿病组和非糖尿病组。在121,575名患者中,有8,532人被归类为糖尿病患者。P-Mg 与 HbA1c 水平≥ 48 mmol/mol 之间呈反向 J 型关系,P-Mg 为 0.90 mmol/L 时为最低点。当 P-Mg 低于 0.90 mmol/L 时,P-Mg 每下降 0.1 mmol/L,首次 HbA1c 测量值≥ 48 mmol/mol 的未调整危险比(HR)为 1.54(95% Cl 1.50;1.57)。当 P-Mg 水平高于 0.90 mmol/L 时,P-Mg 每增加 0.1 mmol/L 的未调整 HR 为 1.04 (95% Cl 1.02; 1.06)。根据年龄和性别进行调整后,HR 仍为 1.06(95% Cl 1.05;1.08)。总之,本研究发现,即使对年龄和性别进行了调整,镁血症患者患糖尿病的风险仍然较高。患者的高镁或低镁血症可能是预测糖尿病发病风险的生物标志物。
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引用次数: 0
Quantifying plasma dacarbazine levels in advanced melanoma patients: a liquid chromatography-tandem mass spectrometry performance analysis. 晚期黑色素瘤患者血浆达卡巴嗪水平的定量分析:液相色谱-串联质谱性能分析。
IF 2.1 4区 医学 Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2024-01-24 DOI: 10.1080/00365513.2023.2297356
Wenhan Cheng, John Liu, Bryan Jackson

The aim of this study was to develop a robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying dacarbazine levels in the plasma of advanced melanoma patients, followed by an assessment of its analytical capabilities. The research encompassed the design of a high-performance liquid chromatography (HPLC) system, with the quantitative analysis performed using the multiple reaction monitoring (MRM) techniques and specific ion transition: 181.0 > 152.5 for dacarbazine and 187.1 > 158.6 for the internal standard (IS), dacarbazine-D6. The validation of the method involved an evaluation of parameters including linearity, detection limit, precision, and accuracy. Notably, the linear range extended from 10 to 1,000 µg/L for dacarbazine, and the method exhibited a detection limit of 10 µg/L. The method's precision, indicated by within-run and between-run coefficients of variation (CV), both being ≤4.2% and ≤8.3%, respectively. Furthermore, the accuracy of measurements, ranging from 86.1% to 99.4%, underscored the method's reliability. In clinical application, the dacarbazine levels of healthy control (n = 20) were 0.6 ± 0.02 μg/L; 770.9 ± 203.2 μg/mL in early-stage-melanoma patients (n = 22), and 588.7 ± 153.2 μg/mL in advanced melanoma patients (n = 25). The results serve as clinical evidence showing that long-term dacarbazine treatment affects the metabolism of dacarbazine.

本研究旨在开发一种稳健的液相色谱-串联质谱(LC-MS/MS)方法,用于定量检测晚期黑色素瘤患者血浆中达卡巴嗪的水平,并对其分析能力进行评估。这项研究包括设计一个高效液相色谱(HPLC)系统,利用多反应监测(MRM)技术和特定离子转换进行定量分析:达卡巴嗪的离子转换率为 181.0 > 152.5,内标(IS)达卡巴嗪-D6 的离子转换率为 187.1 > 158.6。该方法的验证包括对线性、检测限、精密度和准确度等参数的评估。值得注意的是,达卡巴嗪的线性范围为 10 至 1,000 µg/L,方法的检测限为 10 µg/L。该方法的精密度(以运行内变异系数和运行间变异系数表示)分别为≤4.2%和≤8.3%。此外,测量的准确度从 86.1%到 99.4%不等,突出了该方法的可靠性。在临床应用中,健康对照组(20 人)的达卡巴嗪水平为 0.6 ± 0.02 μg/L;早期黑色素瘤患者(22 人)的达卡巴嗪水平为 770.9 ± 203.2 μg/mL;晚期黑色素瘤患者(25 人)的达卡巴嗪水平为 588.7 ± 153.2 μg/mL。这些结果作为临床证据表明,长期达卡巴嗪治疗会影响达卡巴嗪的代谢。
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引用次数: 0
Correction. 更正。
IF 2.1 4区 医学 Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2024-01-24 DOI: 10.1080/00365513.2023.2299904
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引用次数: 0
Vitamin B12 insufficiency and deficiency: a review of nondisease risk factors. 维生素 B12 不足和缺乏:非疾病风险因素综述。
IF 2.1 4区 医学 Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-12-01 Epub Date: 2024-01-24 DOI: 10.1080/00365513.2023.2297357
Omar Abuyaman, Ali Abdelfattah, Faten Shehadeh-Tout, Ahmad A Deeb, Ma'mon M Hatmal

Vitamin B12 deficiency and insufficiency can lead to both hematological and neurological impairments. This review examines nondisease causes and risk factors associated with dietary availability, such as eating habits, food processing, cooking techniques, and bioavailability, as well as increased physiological needs and iatrogenic factors linked to medication use or surgical procedures. As a result of these nondisease influences, groups at higher risk include vegans, vegetarians, older adults, individuals with limited diets, breastfed and preterm infants, and those who primarily consume foods prepared or cooked in ways that reduce vitamin B12 content, as well as individuals on certain medications or who have undergone specific surgeries. Recognizing these diverse risk factors helps develop strategies for prevention and intervention to minimize the adverse health effects related to B12 deficiency and insufficiency.

维生素 B12 缺乏和不足可导致血液和神经系统损伤。本综述探讨了与饮食相关的非疾病原因和风险因素,如饮食习惯、食品加工、烹饪技术和生物利用率,以及与用药或外科手术相关的生理需求增加和先天性因素。由于这些非疾病因素的影响,风险较高的人群包括素食主义者、素食者、老年人、膳食有限的人、母乳喂养的婴儿和早产儿、主要食用以降低维生素 B12 含量的方式烹饪的食物的人,以及服用某些药物或接受过特定手术的人。认识到这些不同的风险因素有助于制定预防和干预策略,最大限度地减少与 B12 缺乏和不足有关的不良健康影响。
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引用次数: 0
The free cortisol calculated: correlation with the free cortisol concentrations measured with liquid chromatography-tandem mass spectrometry after equilibrium dialysis and establishment of reference intervals. 计算游离皮质醇:平衡透析和建立参考区间后,用液相色谱-串联质谱法测定游离皮质醇浓度的相关性。
IF 2.1 4区 医学 Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-11-01 Epub Date: 2023-09-13 DOI: 10.1080/00365513.2023.2256671
Fleur Wolff, Ken Geivaerts, Elise Mathieu, Cécile Duterme, Guillaume Deprez, David Fage, Frédéric Cotton

Background: Changes in cortisol binding globulin (CBG) impact the total serum cortisol concentration and affect the accurate assessment of adrenal function. Free biologically cortisol can be calculated using different equations or directly measured after complicated procedures.

Methods: The free cortisol index (FCI) obtained using the Bonte formula as well as the free cortisol concentration calculated (Coolens equation) were first estimated for 45 healthy workers. The CBG level was determined by a competitive radioimmunoassay and the total cortisol concentration, was measured with an electrochemiluminescent assay. The correlations between FCI, the free cortisol concentrations calculated and the free cortisol levels measured with liquid chromatography-tandem mass spectrometry after equilibrium dialysis were studied for those 45 samples. Reference limits were established on 158 healthy hospital workers and patients with serum samples collected between 7:30 am and 10 am.

Results: The FCI as well as the free cortisol concentrations calculated obtained for the 45 samples correlated significantly with the free cortisol levels measured. Although the cortisol and CBG levels were statistically higher in women using contraceptives compared with women not taking them as well as men, the calculated FCI and free cortisol concentrations did not differ between these groups. The medians (P2.5-P97.5) obtained for the 158 healthy workers were respectively 26.4% (12.3-51.6%) and 10.6 nmol/L (4.3-26.7 nmol/L).

Conclusions: This study highlighted a significant correlation between the FCI, the free cortisol concentrations calculated and the free cortisol levels measured with LC-MS/MS, it has also allowed the establishment of reference intervals for calculated FCI and free cortisol.

背景:皮质醇结合球蛋白(CBG)的变化影响血清总皮质醇浓度,影响肾上腺功能的准确评估。游离的生物皮质醇可以用不同的方程式计算,也可以经过复杂的程序直接测量。方法:首先对45名健康工人进行Bonte公式计算的游离皮质醇指数(FCI)和Coolens方程计算的游离皮质醇浓度进行估计。CBG水平用竞争放射免疫法测定,总皮质醇浓度用电化学发光法测定。研究了平衡透析后45个样品的FCI、计算的游离皮质醇浓度和液相色谱-串联质谱法测定的游离皮质醇水平之间的相关性。对158名健康医院工作人员和患者在上午7:30至10点间采集血清样本建立参考限量。结果:45个样本的FCI以及计算得到的游离皮质醇浓度与测量的游离皮质醇水平显著相关。虽然使用避孕药的女性的皮质醇和CBG水平在统计学上高于不使用避孕药的女性和男性,但计算的FCI和游离皮质醇浓度在这两组之间没有差异。158名健康工人的中位数(p2.5 ~ p97.5)分别为26.4%(12.3 ~ 51.6%)和10.6 nmol/L (4.3 ~ 26.7 nmol/L)。结论:本研究强调了FCI、计算的游离皮质醇浓度和LC-MS/MS测量的游离皮质醇水平之间的显著相关性,并允许建立计算的FCI和游离皮质醇的参考区间。
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引用次数: 0
White matter hyperintensities combined with serum NLRP3 in diagnosis of cognitive impairment in patients with cerebral small vessel disease. 白质高信号联合血清NLRP3对脑血管病患者认知功能障碍的诊断价值
IF 2.1 4区 医学 Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-11-01 Epub Date: 2023-09-13 DOI: 10.1080/00365513.2023.2255974
Ronghui Huang, Lin Zhang, Limeng Deng, Can Chen

Background: White matter hyperintensities (WMH) are widely used for the diagnosis of cerebral small vessel disease (CSVD). However, whether NLRP3 is correlated with cognitive impairment after CSVD is still not clear.Objective: This study aimed to investigate the diagnostic value of WMHs combined with NLRP3 for cognitive impairment after CSVD.Methods: This prospective observational study enrolled a total of 188 CSVD patients from September 2019 to May 2022. All patients received brain MRI assessment and WMH Fazekas score, as well as WMH volume, was recorded. Serum NLRP3 level was measured by ELISA. Patients' cognitive function was measured by MoCA after 6 months of diagnosis of CSVD. The serum levels of C reactive protein (CRP), interleukin (IL)-6, total cholesterol (TC), triglyceride (TG), high-density leptin cholesterol (HDL) and low-density leptin cholesterol (LDL) were recordedResults: CSVD patients with cognitive impairment had significantly higher Fazekas scores, WMH volumes, serum NLRP3 and IL-6 levels compared to patients without cognitive impairment. A positive correlation was found among Fazekas scores, WMH volumes and NLRP3 levels. The combination of WMH volume and NLRP3 could achieve a better specificity for the diagnosis of cognitive impairment. Coronary syndrome history, WMH volume and NLRP3 were found as independent risk factors for cognitive impairment after CSVD.Conclusion: Fazekas scores, WMH volume and serum NLRP3 levels are associated with cognitive impairment after CSVD and have the potential to be used as diagnostic biomarkers.

背景:白质高信号(WMH)被广泛用于脑小血管疾病(CSVD)的诊断。然而,NLRP3是否与CSVD后认知障碍相关尚不清楚。目的:探讨WMHs联合NLRP3对CSVD后认知功能障碍的诊断价值。方法:这项前瞻性观察性研究于2019年9月至2022年5月共纳入188例CSVD患者。所有患者均接受脑MRI评估,并记录WMH Fazekas评分和WMH体积。ELISA法检测血清NLRP3水平。诊断CSVD 6个月后,用MoCA测定患者的认知功能。记录血清C反应蛋白(CRP)、白细胞介素(IL)-6、总胆固醇(TC)、甘油三酯(TG)、高密度瘦素胆固醇(HDL)和低密度瘦素胆固醇(LDL)水平。结果:认知功能障碍CSVD患者Fazekas评分、WMH体积、血清NLRP3和IL-6水平显著高于无认知功能障碍患者。Fazekas评分与WMH体积、NLRP3水平呈正相关。结合WMH体积和NLRP3对认知功能障碍的诊断具有更好的特异性。冠状动脉综合征史、WMH体积和NLRP3是CSVD后认知功能障碍的独立危险因素。结论:Fazekas评分、WMH体积和血清NLRP3水平与CSVD后认知功能障碍相关,具有作为诊断性生物标志物的潜力。
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Scandinavian Journal of Clinical & Laboratory Investigation
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