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Clinical and imaging characteristics of patients with cardiac amyloidosis- a single center observational study. 心脏淀粉样变性患者的临床和影像学特征--一项单中心观察性研究。
IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-05-01 Epub Date: 2024-05-06 DOI: 10.1080/00365513.2024.2346908
Andreas Ingebrigtsen, Sahrai Saeed, Terje Hjalmar Larsen, Håkon Reikvam

Amyloidosis is a disease characterized by the deposition of protein fibrils. Cardiac involvement is a significant factor in determining prognosis. This study aimed to examine the clinical profile, outcomes, and long-term mortality rates in patients with transthyretin (ATTR) and amyloid light-chain (AL) amyloidosis. The retrospective cohort study included 94 patients with amyloidosis (69 with AL and 25 with ATTR amyloidosis) diagnosed between 2010 and 2022. The study involved multimodality imaging (ECG, echocardiography and cardiac magnetic resonance (CMR) data and survival analyses. Patients with ATTR amyloidosis were older and had a higher proportion of males compared to those with AL amyloidosis. Cardiac involvement was more prevalent in the ATTR group, including atrial fibrillation (AF), while pleural and pericardial effusion were more frequent in the AL group. Biomarkers such as NT-proBNP and troponin T were significantly elevated in both groups and were associated with all-cause mortality only in univariate analyses. CMR data, especially typical late gadolinium enhancement (LGE) was not associated with increased mortality, while pleural effusion and left atrial dilatation on echocardiography were identified as powerful predictors of mortality. In conclusion, both AL and ATTR amyloidosis exhibited poor outcomes. Cardiac involvement, particularly dilated left atrium and pleural effusion on echocardiography were associated with an increased risk of mortality, while typical LGE on CMR was not.

淀粉样变性是一种以蛋白质纤维沉积为特征的疾病。心脏受累是决定预后的一个重要因素。本研究旨在探讨转甲状腺素(ATTR)和淀粉样轻链(AL)淀粉样变性患者的临床概况、预后和长期死亡率。这项回顾性队列研究纳入了2010年至2022年期间确诊的94名淀粉样变性患者(69名AL患者和25名ATTR淀粉样变性患者)。研究涉及多模态成像(心电图、超声心动图和心脏磁共振)数据和生存分析。与AL淀粉样变性患者相比,ATTR淀粉样变性患者年龄更大,男性比例更高。心脏受累在ATTR组中更为普遍,包括心房颤动(AF),而胸腔积液和心包积液在AL组中更为常见。两组患者的NT-proBNP和肌钙蛋白T等生物标志物均显著升高,仅在单变量分析中与全因死亡率相关。CMR数据,尤其是典型的晚期钆增强(LGE)与死亡率升高无关,而胸腔积液和超声心动图显示的左心房扩张则是预测死亡率的有力指标。总之,AL 和 ATTR 淀粉样变性的预后都很差。心脏受累,尤其是超声心动图显示的左心房扩张和胸腔积液与死亡风险增加有关,而CMR显示的典型LGE则与之无关。
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引用次数: 0
Correction. 更正。
IF 2.1 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-05-01 Epub Date: 2024-05-31 DOI: 10.1080/00365513.2024.2359217
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引用次数: 0
Measurement uncertainty in clinical chemistry: ISO 20914 versus nordtest or intermediate precision versus bias. 临床化学测量的不确定性:ISO 20914 与 nordtest 或中间精度与偏差。
IF 2.1 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-05-01 Epub Date: 2024-05-14 DOI: 10.1080/00365513.2024.2338738
Nilhan Nurlu, Abdulkadir Cat, Kamil Taha Ucar

Aim: Measuring uncertainty (MU) is crucial to ensure the accuracy and precision of laboratory results. This study compares the ISO 20914 and Nordtest guidelines to analyze the MU values for 20 clinical chemistry analytes over six months.

Methods: The researchers calculated MU components, including within-laboratory reproducibility (Rw), laboratory analytical performance bias (u(bias)), and combined standard uncertainty (uc), based on internal quality control and external quality assessment data. The final expanded uncertainty (U) values were determined by multiplying the combined uncertainty with a coverage factor (k = 2 for 95% Confidence Interval), following each guideline's respective procedures. Clinical chemistry analytes were analyzed on Roche Cobas 6000 c501 auto analyzer (Roche Diagnostics, Mannheim, Germany) and manufacturer's kits were used analysis.

Results: The results show that 11 out of 20 clinical chemistry analytes met the targeted maximum allowable measurement uncertainty (MAU) values when calculated according to ISO 20914 guideline. Also, 11 out of 20 clinical chemistry analytes' MU values met the MAU values with the Nordtest guideline's recommended calculations. However, some tests met the MAU in the ISO 20914 approach but not in the Nordtest guideline, and vice versa.

Conclusions: The study found that intermediate precision (uRw) in the ISO 20914 approach and performance bias (u(bias)) in the Nordtest approach significantly impacted MU values. The research highlights the importance of standardization in MU calculation approaches across clinical laboratories. These findings have implications for patient care and clinical decision-making, emphasizing the importance of selecting appropriate laboratory guidelines for routine use.

目的:测量不确定性(MU)对于确保实验室结果的准确性和精确性至关重要。本研究比较了 ISO 20914 和 Nordtest 准则,分析了 20 种临床化学分析物在六个月内的不确定度值:研究人员根据内部质量控制和外部质量评估数据计算了MU的组成部分,包括实验室内重现性(Rw)、实验室分析性能偏差(u(bias))和综合标准不确定度(uc)。最终的扩展不确定度 (U) 值是按照每份指南各自的程序,将综合不确定度乘以覆盖因子(k = 2,95% 置信区间)确定的。使用罗氏 Cobas 6000 c501 自动分析仪(罗氏诊断公司,德国曼海姆)分析临床化学分析物,并使用制造商提供的试剂盒进行分析:结果表明,根据 ISO 20914 准则计算,20 种临床化学分析物中有 11 种达到了最大允许测量不确定度 (MAU) 的目标值。此外,在 20 个临床化学分析项目中,有 11 个项目的 MU 值符合 Nordtest 指南推荐的 MAU 值。然而,有些检测项目在 ISO 20914 方法中符合 MAU 值,但在 Nordtest 指南中却不符合,反之亦然:研究发现,ISO 20914 方法中的中间精度(uRw)和 Nordtest 方法中的性能偏差(u(bias))对 MAU 值有很大影响。这项研究强调了临床实验室MU计算方法标准化的重要性。这些发现对患者护理和临床决策具有重要意义,强调了选择合适的实验室指南作为常规使用的重要性。
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引用次数: 0
Identification of autoantibodies against PsoP27 in synovial fluid derived from psoriatic arthritis and rheumatoid arthritis patients. 牛皮癣关节炎和类风湿性关节炎患者滑液中针对 PsoP27 的自身抗体的鉴定。
IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-05-01 Epub Date: 2024-05-20 DOI: 10.1080/00365513.2024.2352844
Marina Slobodkin, Ari Polachek, Victoria Furer, Ori Elkayam, Smadar Gertel

PsoP27 is an antigen expressed in psoriatic lesions. It plays an inflammatory role in psoriasis. This study objective was to characterize antibodies (Abs) against PsoP27 in patients with psoriatic arthritis (PsA) and rheumatoid arthritis (RA). Levels of Abs against native and citrullinated PsoP27 in PsA and RA patients' synovial fluid (SF) and sera were determined by ELISA. SF of osteoarthritis (OA) patients and sera of healthy donors were used as controls. Levels of Abs against PsoP27 were correlated with disease activity scores. Abs against native and citrullinated PsoP27 levels in SF of PsA (n = 48; 0.38 ± 0.03 and 0.44 ± 0.04, respectively) and RA (n = 22; 0.57 ± 0.1 and 0.62 ± 0.09, respectively) were significantly higher than in OA patients (n = 23; 0.14 ± 0.01 and 0.15 ± 0.01, respectively) (p < .0001). For both Abs, there were no significant differences between their level in PsA and RA patients. There was no difference in the level of Abs against citrullinated PsoP27 in SF of seronegative versus seropositive RA patients. Levels of Abs against both native and citrullinated PsoP27 in the SF and level of systemic C-reactive protein in PsA correlated positively, while in RA there were no significant correlations with disease activity scores. No differences in level of Abs against PsoP27 were found in the sera of all three study groups. Abs against native and citrullinated PsoP27 are present in PsA and RA SF but not in those of OA patients, suggesting a potential role of those Abs in inflammatory joint diseases.

PsoP27 是一种在银屑病皮损中表达的抗原。它在银屑病中起着炎症作用。本研究旨在确定银屑病关节炎(PsA)和类风湿性关节炎(RA)患者体内针对 PsoP27 的抗体(Abs)的特征。研究采用 ELISA 方法测定了 PsA 和 RA 患者滑液(SF)和血清中针对原生和瓜氨酸化 PsoP27 的抗体水平。骨关节炎(OA)患者的滑液和健康供体的血清作为对照。针对 PsoP27 的抗体水平与疾病活动度评分相关。PsA(n = 48;分别为 0.38 ± 0.03 和 0.44 ± 0.04)和 RA(n = 22;分别为 0.57 ± 0.1 和 0.62 ± 0.09)患者 SF 中针对原生和瓜氨酸化 PsoP27 的 Abs 水平明显高于 OA 患者(n = 23;分别为 0.14 ± 0.01 和 0.15 ± 0.01)(p<0.05)。
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引用次数: 0
Early prediction of sepsis-induced respiratory tract infection using a biomarker-based machine-learning algorithm. 利用基于生物标志物的机器学习算法早期预测败血症诱发的呼吸道感染。
IF 2.1 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-05-01 Epub Date: 2024-04-29 DOI: 10.1080/00365513.2024.2346914
Mingkuan Su, Haiying Wu, Hongbin Chen, Jianfeng Guo, Zongyun Chen, Jie Qiu, Jiancheng Huang

Early and differential diagnosis of sepsis is essential to avoid unnecessary antibiotic use and further reduce patient morbidity and mortality. Here, we aimed to identify predictors of sepsis and advance a machine-learning strategy to predict sepsis-induced respiratory tract infection (RTI). Patients with sepsis and RTI were selected via retrospective analysis, and essential population characteristics and laboratory parameters were recorded. To improve the performance of the primary model and avoid over-fitting, a recursive feature elimination with cross-validation (RFECV) strategy was used to screen the optimal subset of biomarkers and construct nine machine-learning models based on this subset; the average accuracy, precision, recall, and F1-score were used for evaluation of the models. We identified 430 patients with sepsis and 686 patients with RTI. A total of 39 features were collected, with 23 features identified for initial model construction. Using the RFECV algorithm, we found that the XGBoost classifier, which only needed to include seven biomarkers, demonstrated the best performance among all prediction models, with an average accuracy of 89.24 ± 2.28, while the Ridge classifier, which included 11 biomarkers, had an average accuracy of only 83.87 ± 4.69. The remaining models had prediction accuracies greater than 88%. We developed nine models for predicting sepsis using a strategy that combined RFECV with machine learning. Among these models, the XGBoost classifier, which included seven biomarkers, showed the best performance and highest accuracy for predicting sepsis and may be a promising tool for the timely identification of sepsis.

脓毒症的早期鉴别诊断对于避免不必要的抗生素使用和进一步降低患者发病率和死亡率至关重要。在此,我们旨在确定败血症的预测因素,并推进一种机器学习策略,以预测败血症诱发的呼吸道感染(RTI)。我们通过回顾性分析筛选出脓毒症和 RTI 患者,并记录了基本人群特征和实验室参数。为了提高主要模型的性能并避免过度拟合,我们采用了递归特征消除与交叉验证(RFECV)策略来筛选最佳生物标志物子集,并基于该子集构建了九个机器学习模型;模型的评估采用了平均准确度、精确度、召回率和 F1 分数。我们确定了 430 名败血症患者和 686 名 RTI 患者。共收集了 39 个特征,其中 23 个特征被确定用于构建初始模型。使用 RFECV 算法,我们发现在所有预测模型中,只需包含 7 个生物标记物的 XGBoost 分类器表现最佳,平均准确率为 89.24 ± 2.28,而包含 11 个生物标记物的 Ridge 分类器的平均准确率仅为 83.87 ± 4.69。其余模型的预测准确率均超过 88%。我们采用 RFECV 与机器学习相结合的策略开发了九种预测败血症的模型。在这些模型中,包含 7 个生物标记物的 XGBoost 分类器在预测败血症方面表现最佳,准确率最高,可能是及时识别败血症的一种有前途的工具。
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引用次数: 0
End-user verification results of two serum separator tubes for clinical chemistry analytes according to CLSI GP34-A and CLSI GP41-A6. 根据 CLSI GP34-A 和 CLSI GP41-A6 对两种血清分离管进行临床化学分析的最终用户验证结果。
IF 2.1 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-05-01 Epub Date: 2024-05-03 DOI: 10.1080/00365513.2024.2344681
Sedat Abusoglu, Busra Ecer, Halil Guven, Ahmet Emre Yorulmaz, Muhittin Abdulkadir Serdar, Ali Unlu, Fikret Akyurek, Bahadir Ozturk, Gulsum Abusoglu

Tube manufacturers use different composition of gels and blood clot activator formulations in serum tube production. Our aim was to investigate the within-tube (repeatability) and between-tube variation, concordance between comparison results of BD and VacuSEL tubes. Blood samples were collected from control subjects (n = 20) and patients (n = 30) in accordance with the CLSI GP41-A6 and CLSI GP34-A guidelines. Twenty-three clinical chemistry parameters were analysed via Roche Cobas C702 Chemistry Analyzer on T0 (0 hour) and T24 (24 hour). Mean differences % were compared with Wilcoxon matched pair test. Clinical significance was evaluated based on desirable bias according to total allowable error (TEa). VacuSEL tubes demonstrated acceptable performance for the results of 20 parameters with regards to desirable bias % limits. Lactate dehydrogenase (LD) [mean difference % (%95 confidence intervals (CI) values of BD and VacuSEL tubes at T0 [6.41% (4.80-8.01%)]; sodium (Na) and total protein (TP) at T24 [-0.27% (-0.46 to -0.07%) and -1.39% (-1.87 to -0.91), respectively] were over the desirable bias limits (LD: 4.3%, Na: 0.23% and TP: 1.36%, respectively) but not exceeding total biological variation CV % [Na: 0.5 (0.0-1.0) % and TP: 2.6 (2.3-2.7) %). %95 confidence intervals (CI) of T0 LD values overlap with within-subject biological variation % (CI) limits (LD: 5.2 (4.9-5.4) %). The differences between two tubes were not medically significant and necessarily conclusive. VacuSEL serum tubes presented comparable performance with BD serum tubes.

试管制造商在生产血清试管时使用不同的凝胶和血凝块活化剂配方。我们的目的是研究 BD 和 VacuSEL 血清试管的管内(重复性)和管间差异以及对比结果的一致性。根据 CLSI GP41-A6 和 CLSI GP34-A 指南,我们采集了对照组(20 人)和患者(30 人)的血样。在 T0(0 小时)和 T24(24 小时)期间,通过罗氏 Cobas C702 化学分析仪分析了 23 项临床化学参数。采用 Wilcoxon 配对检验比较平均差%。临床意义根据总允许误差(TEa)的理想偏差进行评估。VacuSEL 管在 20 个参数的结果中表现出了可接受的性能,达到了理想的偏差百分比限值。乳酸脱氢酶 (LD) [T0 时 BD 和 VacuSEL 管的平均差% (%95 置信区间 (CI) 值 [6.41% (4.80-8.01%)];T24 时钠 (Na) 和总蛋白 (TP) [-0.27% (-0.46 至 -0.07%) 和 -1.分别为-0.27%(-0.46 至-0.07%)和-1.39%(-1.87 至-0.91)]超过了理想偏差限(分别为 LD:4.3%、Na:0.23% 和 TP:1.36%),但未超过总生物变异 CV % [Na:0.5 (0.0-1.0) % 和 TP:2.6 (2.3-2.7) %]。T0 LD 值的 95%置信区间 (CI) 与受试者内生物变异% (CI) 限值重叠(LD:5.2 (4.9-5.4) %)。两种试管之间的差异不具有医学意义,也不一定是结论性的。VacuSEL 血清管与 BD 血清管的性能相当。
{"title":"End-user verification results of two serum separator tubes for clinical chemistry analytes according to CLSI GP34-A and CLSI GP41-A6.","authors":"Sedat Abusoglu, Busra Ecer, Halil Guven, Ahmet Emre Yorulmaz, Muhittin Abdulkadir Serdar, Ali Unlu, Fikret Akyurek, Bahadir Ozturk, Gulsum Abusoglu","doi":"10.1080/00365513.2024.2344681","DOIUrl":"10.1080/00365513.2024.2344681","url":null,"abstract":"<p><p>Tube manufacturers use different composition of gels and blood clot activator formulations in serum tube production. Our aim was to investigate the within-tube (repeatability) and between-tube variation, concordance between comparison results of BD and VacuSEL tubes. Blood samples were collected from control subjects (n = 20) and patients (n = 30) in accordance with the CLSI GP41-A6 and CLSI GP34-A guidelines. Twenty-three clinical chemistry parameters were analysed via Roche Cobas C702 Chemistry Analyzer on T0 (0 hour) and T24 (24 hour). Mean differences % were compared with Wilcoxon matched pair test. Clinical significance was evaluated based on desirable bias according to total allowable error (TEa). VacuSEL tubes demonstrated acceptable performance for the results of 20 parameters with regards to desirable bias % limits. Lactate dehydrogenase (LD) [mean difference % (%95 confidence intervals (CI) values of BD and VacuSEL tubes at T0 [6.41% (4.80-8.01%)]; sodium (Na) and total protein (TP) at T24 [-0.27% (-0.46 to -0.07%) and -1.39% (-1.87 to -0.91), respectively] were over the desirable bias limits (LD: 4.3%, Na: 0.23% and TP: 1.36%, respectively) but not exceeding total biological variation CV % [Na: 0.5 (0.0-1.0) % and TP: 2.6 (2.3-2.7) %). %95 confidence intervals (CI) of T0 LD values overlap with within-subject biological variation % (CI) limits (LD: 5.2 (4.9-5.4) %). The differences between two tubes were not medically significant and necessarily conclusive. VacuSEL serum tubes presented comparable performance with BD serum tubes.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"183-192"},"PeriodicalIF":2.1,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140863801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reproducibility and stability of the immature platelet fraction using Sysmex XN-10. 使用 Sysmex XN-10 检测未成熟血小板部分的可重复性和稳定性。
IF 2.1 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-04-01 Epub Date: 2024-02-29 DOI: 10.1080/00365513.2024.2321590
Usman Ali, Gavin Knight, Mridula Chopra, Dimitris A Tsitsikas

Background: The Immature Platelet Fraction (IPF) is an indicator of thrombopoiesis which is a useful parameter in thrombocytopenia. It demonstrates compensatory mechanisms in production of platelets, but currently not implemented in routine clinical practice. The aim of this study was to establish the reproducibility and stability of IPF, for both percentage (%-IPF) and absolute (A-IPF) measurements.Material/methods: A total of 71 samples, of which 45 for reproducibility and 26 for stability analysis, were assayed for full blood count using the Sysmex XN-10 analyser at room temperature (RT:19-25 °C). For reproducibility analysis, IPF measurements were analysed 11 times by different appraisers using the same sample, while for stability analysis, IPF was measured over fourteen hourly-intervals up to 24 h (n = 21) and then separately extended beyond the point of stability to 72 h (n = 5).

Results: Reproducibility analysis of %-IPF and A-IPF (n = 45) showed very reliable results, with the range of mean CV% values between 1.25-8.90% and 1.70-9.96%, respectively. On the other hand, overall, stability analysis of %-IPF and A-IPF (n = 21) at RT over 24 h showed reliable results, with pooled mean CV% values of 1.32% and 1.43%, respectively, with no significant difference between %-IPF and A-IPF (p = 0.767 and p = 0.821). All %-IPF and A-IPF values had exceeded the set acceptance criterion of stability (CV% ≥ 10.0%) before 72 h.

Conclusions: Overall, %-IPF and A-IPF reproducibility and storage at RT for 24 h predominantly demonstrates the suitability of their usage for testing on the Sysmex XN-series analysers.

背景:未成熟血小板比例(IPF)是血小板生成的一个指标,是血小板减少症的一个有用参数。它显示了血小板生成的代偿机制,但目前尚未在常规临床实践中应用。本研究旨在确定 IPF 百分比(%-IPF)和绝对值(A-IPF)测量的可重复性和稳定性:使用 Sysmex XN-10 分析仪在室温(RT:19-25 °C)下对 71 份样本进行了全血细胞计数分析,其中 45 份用于重现性分析,26 份用于稳定性分析。在重现性分析中,由不同的鉴定人员使用同一样本进行了 11 次 IPF 测量分析;而在稳定性分析中,IPF 的测量时间间隔为每小时 14 次,直至 24 小时(n = 21),然后在稳定点之后分别延长至 72 小时(n = 5):结果:%-IPF 和 A-IPF 的重现性分析(n = 45)显示结果非常可靠,平均 CV% 值范围分别为 1.25-8.90% 和 1.70-9.96%。另一方面,总体而言,RT 24 小时内 %-IPF 和 A-IPF(n = 21)的稳定性分析结果可靠,汇总平均 CV% 值分别为 1.32% 和 1.43%,%-IPF 和 A-IPF 之间无显著差异(p = 0.767 和 p = 0.821)。所有 %-IPF 和 A-IPF 值在 72 小时前都超过了设定的稳定性接受标准(CV% ≥ 10.0%):总体而言,%-IPF 和 A-IPF 的重现性和在 RT 条件下保存 24 小时的稳定性表明,它们适合在 Sysmex XN 系列分析仪上进行测试。
{"title":"Reproducibility and stability of the immature platelet fraction using Sysmex XN-10.","authors":"Usman Ali, Gavin Knight, Mridula Chopra, Dimitris A Tsitsikas","doi":"10.1080/00365513.2024.2321590","DOIUrl":"10.1080/00365513.2024.2321590","url":null,"abstract":"<p><strong>Background: </strong>The Immature Platelet Fraction (IPF) is an indicator of thrombopoiesis which is a useful parameter in thrombocytopenia. It demonstrates compensatory mechanisms in production of platelets, but currently not implemented in routine clinical practice. The aim of this study was to establish the reproducibility and stability of IPF, for both percentage (%-IPF) and absolute (A-IPF) measurements.<b>Material/methods:</b> A total of 71 samples, of which 45 for reproducibility and 26 for stability analysis, were assayed for full blood count using the Sysmex XN-10 analyser at room temperature (RT:19-25 °C). For reproducibility analysis, IPF measurements were analysed 11 times by different appraisers using the same sample, while for stability analysis, IPF was measured over fourteen hourly-intervals up to 24 h (<i>n</i> = 21) and then separately extended beyond the point of stability to 72 h (<i>n</i> = 5).</p><p><strong>Results: </strong>Reproducibility analysis of %-IPF and A-IPF (<i>n</i> = 45) showed very reliable results, with the range of mean CV% values between 1.25-8.90% and 1.70-9.96%, respectively. On the other hand, overall, stability analysis of %-IPF and A-IPF (<i>n</i> = 21) at RT over 24 h showed reliable results, with pooled mean CV% values of 1.32% and 1.43%, respectively, with no significant difference between %-IPF and A-IPF (<i>p</i> = 0.767 and <i>p</i> = 0.821). All %-IPF and A-IPF values had exceeded the set acceptance criterion of stability (CV% ≥ 10.0%) before 72 h.</p><p><strong>Conclusions: </strong>Overall, %-IPF and A-IPF reproducibility and storage at RT for 24 h predominantly demonstrates the suitability of their usage for testing on the Sysmex XN-series analysers.</p>","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"91-96"},"PeriodicalIF":2.1,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139991034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Red blood cell distribution width (RDW) reflects disease severity in patients with carbon monoxide poisoning: systematic literature review and meta-analysis. 红细胞分布宽度 (RDW) 反映一氧化碳中毒患者的疾病严重程度:系统文献综述和荟萃分析。
IF 2.1 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-04-01 Epub Date: 2024-03-28 DOI: 10.1080/00365513.2024.2332998
Giuseppe Lippi, Brandon M Henry, Camilla Mattiuzzi

No definitive prognostic biomarkers for carbon monoxide (CO) poisoning have been proposed. The aim of this study is to investigate, through a systematic literature review and pooled analysis, whether red blood cell distribution width (RDW) can predict disease severity in CO-poisoned patients. We performed an electronic search in Scopus and PubMed using the keywords: 'red blood cell distribution width' OR 'RDW' AND 'carbon monoxide' AND 'poisoning,' with no time or language restrictions (i.e. through August 2023) to find clinical studies that examined the value of RDW in patients with varying severity of CO poisoning. The analysis was performed according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) 2020 reporting checklist. We identified 29 articles, seven of which were included in our analysis, with a total of 1979 CO-poisoned patients, 25.9% of whom were severely ill. In all but one of the studies, the RWD mean or median value was higher in CO-poisoned patients with severe disease. The weighted mean difference (WMD) of RDW was 0.36 (95% confidence interval (CI), 0.26-0.47)%. In the three articles in which the severity of illness in CO-poisoned patients was defined as cardiac injury, the WMD of the RDW was 1.26 (95%CI, 1.02-1.50)%. These results suggest that monitoring RDW in CO-poisoned patients may help to determine the severity of disease, particularly cardiac injury.

目前尚未提出一氧化碳(CO)中毒的明确预后生物标志物。本研究旨在通过系统性文献综述和汇总分析,探讨红细胞分布宽度(RDW)能否预测一氧化碳中毒患者的疾病严重程度。我们使用关键字在 Scopus 和 PubMed 上进行了电子检索:红细胞分布宽度 "或 "RDW"、"一氧化碳 "和 "中毒",没有时间或语言限制(即截止到 2023 年 8 月),以查找在不同严重程度的一氧化碳中毒患者中检测 RDW 价值的临床研究。分析按照 PRISMA(系统综述和元分析首选报告项目)2020 报告清单进行。我们共发现了 29 篇文章,其中 7 篇纳入了我们的分析,共涉及 1979 名一氧化碳中毒患者,其中 25.9% 的患者病情严重。除一项研究外,其他所有研究中,病情严重的一氧化碳中毒患者的 RWD 平均值或中位值均较高。RDW 的加权平均差 (WMD) 为 0.36(95% 置信区间 (CI),0.26-0.47)%。在将一氧化碳中毒患者的病情严重程度定义为心脏损伤的三篇文章中,RDW 的加权平均差值为 1.26(95%CI,1.02-1.50)%。这些结果表明,监测一氧化碳中毒患者的 RDW 可能有助于确定疾病的严重程度,尤其是心脏损伤。
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引用次数: 0
LC-MS/MS analysis of 11-nor-9-carboxy-hexahydrocannabinol (HHC-COOH) and 11-hydroxy-hexahydrocannabinol (HHC-OH) for verification of hexahydrocannabinol (HHC) intake. 对 11-去甲-9-羧基六氢大麻酚(HHC-COOH)和 11-羟基六氢大麻酚(HHC-OH)进行 LC-MS/MS 分析,以核实六氢大麻酚(HHC)的摄入量。
IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-04-01 Epub Date: 2024-03-26 DOI: 10.1080/00365513.2024.2333023
Paul Pettersson-Pablo, Joakim Oxelbark

Natural and semi-synthetic cannabinoid analogs are getting increasing media attention for their recreative use as an alternative to traditional cannabis, in Sweden as well as internationally. To investigate an increasing number of urine samples incoming to our clinical laboratory that were screening positive, using a CEDIA THC-COOH immunoassay from ThermoFisher Scientific, but then testing negative using GC-MS based verification analysis, we developed an LC-MS/MS-method for verification of hexahydrocannabinol (HHC) and Δ8-tetrahydrocannabinol. Assessment of HHC intake was based on identification of the following four metabolites: 11-nor-9(R)-carboxy-hexahydrocannabinol (R-HHC-COOH), 11-nor-9(S)-carboxy-hexahydrocannabinol (S-HHC-COOH), 11-hydroxy-9(R)-hexahydrocannabinol (R-HHC-OH) and 11-hydroxy-9(S)-hexahydrocannabinol (S-HHC-OH). Out of 46 urine samples analysed in this study, 44 showed presence of HHC-metabolites, which indicate HHC as the main explanation for an increased number of negative verifications for THC-COOH. In these samples, the HHC-OH metabolites occurred at a higher concentration than R-HHC-COOH while S-HHC-COOH was only detected in few samples at low concentrations. R-HHC-COOH and S-HHC-COOH can easily be added to a pre-existing verification method for THC-COOH, and still show acceptable results, while HHC-OH requires an enzyme capable of hydrolysing the ether glucuronide bond.

在瑞典和国际上,天然和半合成大麻素类似物因其作为传统大麻替代品的娱乐用途而日益受到媒体关注。我们的临床实验室收到越来越多的尿样,使用 ThermoFisher Scientific 公司的 CEDIA THC-COOH 免疫测定法检测结果呈阳性,但使用基于 GC-MS 的验证分析法检测结果呈阴性。HHC 摄入量的评估基于以下四种代谢物的鉴定:11-去甲-9(R)-羧基六氢大麻酚(R-HHC-COOH)、11-去甲-9(S)-羧基六氢大麻酚(S-HHC-COOH)、11-羟基-9(R)-六氢大麻酚(R-HHC-OH)和 11-羟基-9(S)-六氢大麻酚(S-HHC-OH)。在本研究分析的 46 份尿样中,44 份显示存在 HHC 代谢物,这表明 HHC 是导致 THC-COOH 阴性验证数量增加的主要原因。在这些样本中,HHC-OH 代谢物的浓度高于 R-HHC-COOH,而 S-HHC-COOH 仅在少数样本中检测到,且浓度较低。R-HHC-COOH 和 S-HHC-COOH 可以很容易地添加到已有的 THC-COOH 检验方法中,并仍然显示出可接受的结果,而 HHC-OH 则需要一种能够水解醚葡萄糖醛酸键的酶。
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引用次数: 0
Pediatric reference intervals for serum folate and cobalamin based on a European population without exposure to folic acid fortification. 基于未接触叶酸强化剂的欧洲人口的儿科血清叶酸和钴胺素参考区间。
IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2024-04-01 Epub Date: 2024-03-21 DOI: 10.1080/00365513.2024.2330918
Peter Ridefelt, Johan Saldeen, Mandy Vogel, Uta Ceglarek, Wieland Kiess, Anders Larsson

The aim of the present study was to define pediatric reference intervals for serum cobalamin and folate utilizing data generated from a population not exposed to food fortified with folic acid. Folate and cobalamin results analyzed by electrochemiluminescence immunoassay (Roche Cobas) were obtained from 2375 children (2 months to 17.99 years of age). The serum samples were collected between 2011 and 2015 as part of the LIFE (Leipzig Research Centre for Civilization Diseases) Child cohort study in Germany, where folic acid fortification of food is not mandated. These results were used to generate age- and gender-specific reference intervals presented as non-parametric 2.5 and 97.5 percentiles. Because of a subsequent restandardisation of the Roche folate assay in 2016, folate values were recalculated accordingly for adaptation to results obtained using the present calibration. In both genders, folate concentrations decreased continuously with age, whereas cobalamin concentrations peaked at five years of age and then declined. Teenage females had higher concentrations of cobalamin in the age group 12-17.99 years.

本研究旨在利用未接触叶酸强化食品人群的数据,确定儿科血清钴胺素和叶酸的参考区间。通过电化学发光免疫分析法(罗氏 Cobas)对 2375 名儿童(2 个月至 17.99 岁)的叶酸和钴胺素结果进行了分析。这些血清样本是在 2011 年至 2015 年期间收集的,是德国 LIFE(莱比锡文明病研究中心)儿童队列研究的一部分。这些结果被用来生成特定年龄和性别的参考区间,以非参数的 2.5 和 97.5 百分位数表示。由于罗氏叶酸测定法随后于2016年重新标准化,因此叶酸值也相应地重新计算,以适应使用本校准法获得的结果。在男女两性中,叶酸浓度随着年龄的增长而持续下降,而钴胺素浓度则在五岁时达到峰值,然后下降。在 12-17.99 岁年龄组中,青少年女性的钴胺素浓度较高。
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Scandinavian Journal of Clinical & Laboratory Investigation
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